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  • 101
    Publication Date: 2021-07-16
    Description: This project has done with the collaboration of Bnyad mostazafan (Ran agriculture and livestock Co.) with goal of increasing efficiency of warm water fishes (Chinese Carps) production. The fish were purchased from China (B. G.) in two parts in Jun 2014. The fish then transferred and quarantined to the ponds in Aghili’s farm In Azadshar using container trucks. The biological parameters of fish such as growth rate were measured during the experiment. The growth rate were obtained 9, 16, 16 and 14% for common carp, big head, silver carp and grass carp, respectively. The result shows that production of experimental ponds (ponds 2 and 4) were obtained 3473 kg/ha while the control ponds produced 2700kg/ha 1n 1393. In general the production were increased 31% when comparing with average of local production and raised 26% when comparing with average production of the entire country in 1393. In this study showed that by importing the F1 generation of Chinese carp, it can expected that production will be increased to 1.5 times of current carrying capacity as long as using aeration system and appropriate management.
    Keywords: Aquaculture ; Biology ; Iran ; Survey ; Carp ; Species ; Genetic ; Diversity ; Population
    Repository Name: AquaDocs
    Type: monograph
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  • 102
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25668 | 18721 | 2018-10-14 03:05:49 | 25668 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Aspects of the biological features such as age and growth, the reproductive cycle , food and feeding of the Longnose Trevally (Carangoides chrysophrys) were studied from a total 376 specimens collected by use of trawl fishing in Hormuzgan waters between February 2014 and February 2015. The minimum and maximum total length during different months were between 25.5 and 80 cm respectively. Weight-length relationship for Longnose Trevally was W= 0/0064L 2/9004. This fish had an isometric growth. Fishes aged using sections of their otoliths. The equation of growth for Longnose Trevally obtained Lt=85(1-e-0/266(t+1/443)). Total mortality rate for Longnose Trevally was 0.412. LM50 and TM50 for Longnose Trevally was 46 cm, 2 years. Sex ratio(femail : male) for Longnose Trevally was 1/42:1. Maximum absolute and relative fecundity for Longnose Trevally were 479992 and 354 respectively. The highest GSI in April (2.86) and the lowest was in June 2014 (0.43). It has a long spawning season from January to May and spawning peak was observed in May. Longnose Trevally was Relatively low feed (CV= 65.49). Main food for Longnose Trevally were bony fish (Fp= 91.67). Random diet of Longnose Trevally were crustaceans (Fp=4.17) (shrimp, crab and squilla) and mollusks (Fp=4.17) (cutlle fish, Squid), respectively.
    Keywords: Biology ; Fisheries ; Iran ; Hormuzgan ; Hormozgan waters ; Longnose Trevally ; Parameters ; Growth ; Spawning ; Season ; Length ; Mature ; Fecundity ; Feeding ; Sex raio ; Carangoides chrysophrys ; Biological
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  • 103
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25672 | 18721 | 2018-10-14 03:00:20 | 25672 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Gammarus (Crustacea, Amphipoda), widely distributed in various geographical areas in Iran, is one of the important candidates for artificial production for using in fish food. So far, the ecology of this crustacean, its distribution map in Iran, and the probability of its production in artificial conditions has been investigated. For finding a suitable method to make its production investments beneficial, it is needed to precisely investigate reproduction cycles and growth, to find ways for reaching their maximum production. Two sub-population of G. pseudosyriacus was monthly sampled in two different stations in Arjan spring, Arjan Protected Area, Fars Province in 2006. The two stations differed mainly in their distance to the spring source and to the roadside stores and restaurants. Sex ratios, number of ovigerous females, adult females, clutch size (eggs per brood pouch), egg volume, and the population composition were counted or measured. Fecundity index (FI) and reproductive effort (RE) were calculated using these basic data. Dissolved oxygen, air and water temperature, pH, and electrical conductivity were measured in the field; calcium, magnesium, potassium, and total hardness were measured in laboratory. Station 2 showed higher water temperature, dissolved oxygen, and ionic content. The number of populations was maximum in late winter and minimum in late summer. Both populations produced fewer eggs in autumn, and there were more non-ovigerous females in autumn-winter, while more ovigerous in spring-summer. Males and females of station 1showed a higher number, however, in both stations the sex ratio by weight favored males. Both sexes showed increased weights in spring and summer months and male samples had higher weights in station 1 compared to station 2. Females of station 1 produced eggs of the twice volume, while decreasing clutch size. Both populations showed higher fecundity index in late winter and early spring, which was higher in overall in station 2. As many other Gammarus species, the two subpopulation of G. pseudosyriacus had a continuous reproduction throughout the year. Seasonal patterns of population numbers suggest that the strategy of this population is to increase number in winter and weight in summer. It was suggested that both sub-populations evaluate late-winter to early spring as more suitable, therefore do better investment to produce more offspring in this time period. On the other hand, higher fecundity index in station 2 suggests lower stability of this habitat. Individuals in this habitat expend their energy in production of more eggs to increase the chance of their offspring survival to maintain the population size in a maximum possible level. Females of sub-population 1 produced fewer eggs, but they made more investment of resources for each egg. This strategy indicates higher stability in suitable conditions of the hosting environment.
    Keywords: Biology ; Ecology ; Iran ; Amphipod ; Gammarus ; Reproductive cycle ; Fecundity index ; Reproductive effort
    Repository Name: AquaDocs
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  • 104
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25688 | 18721 | 2018-10-08 09:12:04 | 25688 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Jinga shrimp Metapenaeus affinis is one of the most important penaeide shrimp species in Persian Gulf that has the highest amount of shrimp catch after banana shrimp in Hormozgan province. Regarding the importance of Jinga shrimp in fisheries of this province, genetic diversity and population structure of this species was assessed for the first time by mitochondrial 16SrRNA sequencing. A number of 18 shrimps were collected from the regions of Bandar Abbas, Bushehr and Khuzestan (six samples each region). DNA Extraction was performed using phenolchloroform and by optimizing the PCR for amplification of 16SrRNA, the most suitable temperature for primer binding was 48 °C. Analysis of 18 sequenced samples including 486 aligned base pairs of 16SrRNA yielded 480 monomorphic loci, 6 polymorphic loci and 2 transitions. No insertions and deletions were observed. 9 haplotypes were identified from the 18 samples. Mean haplotype diversity in each region was recorded from 0.0 ± 0.0 (Bandar Abbas) to 0.333 ± 0.215 (Bushehr) and 0.333 ± 0.215 (Khuzestan), and mean nucleotide diversity from 0.0 ± 0.0 (Bandar Abbas) to 0.003 ± 0.003 (Bushehr) and 0.001 ± 0.001 (Khuzestan). Haplotype and nucleotide diversity of all samples were 0.608 ± 0.007 and 0.002 ± 0.003, respectively. The maximum amount of F- statistic parameter was 0.750 between samples of Bandar Abbas and Khuzestan and the minimum amount between Bushehr and Khuzestan (-0.105). At probability level of 0.05, population differentiation was significant between Bandar Abbas and two other regions of Bushehr and Khuzestan but not significant between regions of Bushehr and Khuzestan. Test of exact p values within population confirmed the difference of Bandar Abbas population from the two other populations. Phylogenetic trees showed the differentiation of Bandar Abbas population from the two other regions. The results of this study using mitochondrial 16SrRNA sequencing revealed that the Jinga shrimp population of Bandar Abbas is a differentiated and separated gene pool from the two other regions, and although the populations of Bushehr and Khuzestan seem not genetically separated, molecular diversity of this species is acceptable in these two regions.
    Keywords: Biology ; Fisheries ; Iran ; Persian Gulf ; Sequencing ; Population structure ; 16SrRNA ; Jinga shrimp ; Metapenaeus affinis
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  • 105
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25687 | 18721 | 2018-10-08 10:51:13 | 25687 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The purpose of this study was to investigate the possibility of natural reproduction of indanger fish in the southern Caspian Sea in Tajan, Haraz,Sardabrood and Chalos rivers, and identify suitable habitats for this species in the study area as a genetic reserve.Sampling of physical and chemical parameters of water, macrobenthic invertebrates and fish fauna was conducted seasonaly in each rivers at selected stations from febrary 2010 during one year. Macrobenthic invertebrate sampling were used bySorber sampler and fish was collected by gill net and electroshocker set with a voltage of 1.7 A and 100-300 V. A total of 53 taxa of macrobenticinvertabrateshave been identified in the four mentioned rivers, in which Tajanriverconsisted of 47 taxa with highest richness.20 species of fish were determined in the studied rivers that 6 of them were non-native species.The results showed that the predominant identified fish species were native in the river. The fish species diversity increases as one moves from the headstream towards the estuaries of the two studied Sardabrud and Chalus rivers.The results of this study showed that the relative abundance of fish species resistant to pollution and hard environmental conditions were much more that sensitive species. physicochemical parameters of water such as; water temperature, DO, BOD5, pH, TSS, TDS, EC, turbidity, nutrients, biodiversity index such as Shanon index, EPT, HFBI, EPT/CHIR, nutrition functioning group and present or absent of fish species showed that there are significant decrease in water qualities from upstraem ratio to down stream (For example, in Tajan river water turbidity varied from 0 at station 1 to 333 NTU at station 6, Which is strongly influenced by sand workshops. As well as the activities of a significant impact of fish farming workshop on the Haraz river water quality). The minimum of HFBI index was 2.84 at station 1 and the maximum was recorded at station 5 with 6.01, and there is not any possibility to natural reproduction of migrated species that are dominant and indanger at downstream. Conditions for natural reproduction of this species in the rivers of srdabrood and Chalus there is to some. In Shahid- Rajaei dam the maximum relative abundance of fish species belonged to Capoeta capoeta, Luciobarbus capito and Squalius cephalus, respectively. These species have different age, length and weight stuctures at Shahid- Rajaei dam inhabitant, and the resourval of this dam and it,s upstream have been preparing auch a favorabile condition that seems there are potentioal of introductionand inhabitant of some of the Migratory and indanger fish species such as Barbels, Sturgeon, Alburnus chalcoides and etc. as genetic reserve for biodiversity preservation.
    Keywords: Biology ; Fisheries ; Iran ; Tajan ; Chalos rivers ; Haraz ; Sardabrood ; Inhabitant ; Pollution ; Rehabilitation ; Indanger fish species ; Alburnus chalcoides ; Squalius cephalus ; Luciobarbus capito ; Capoeta capoeta
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  • 106
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25693 | 18721 | 2018-10-08 09:36:10 | 25693 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Inthisstudy, Iranian and French male and female Oncorhynchus mykiss broodstocks were invided into two groups 50 and 24 respectivelyin Research center of genetic and breeding of coldwater fishers, Yasouj,Iran and the genetic structure of them was investigated using 6 microsatellite markers. Along with broodstock maturation, fertilization 1:1(female:male) were randomly assigned and occurred in25 of12Iranian andFrench treatment respectively. Reproductive parameters were recordedfor the whole family.Average number of observed alleles in Iranian and French stocks was 6.68 and 6.83, respectively.Average number of effective alleles in Iranian and French stocks was 3.13 and 3.45 respectively.Fixation index Fstwas calculated based on allelic frequency between two stocks was 0.058 with significant difference between 2 stocks. Eyed percentage for french broodstock calaulated zero and deleted. Fertilization rate(100-0), the eyed percentage (98-0), The hatch rate(98- 0),the averagefecundity4114.708, the average eggs size 4.88 mm, Survivalinthe first three months 19-73% calculated for Iranian broodstocks. Considering the quality of eggs and larvaeat different stages and selection between the different family and the within family remained 10 treatments and are keptas future broodstocks. Therelationship between fecundity-egg size, fecundity-weight, fecundity-length, egg sizeweightwas performed using regression. The results showed that Fecundity wasinfluenced more byweight and productivelength. The research is beginning to IDthe broodstockin our country.
    Keywords: Biology ; Iran ; Oncorhynchus mykiss ; Genetic ; Selection
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  • 107
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25713 | 18721 | 2018-10-10 10:11:15 | 25713 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Chitin nanofibers are prepared from the exoskeletons of shrimp by a simple mechanical treatment after a series of purification steps. The nanofibers have fine nanofibers networks with a uniform width of approximately 10 nm. Grinders and highpressure water jet systems are effective for disintegrating chitin into nanofibers. Acidic conditions are the key factor to facilitate mechanical fibrillation. Ultrafine fibers were successfully fabricated chitosan and fish skinextracted gelatin via electrospinning (ES). Important ES parameters, such as concentration of aqueous acid and fish gelatin solutions, and electric field intensity were examined to investigate the effects on the morphology of the gelatin nanofibers. Due to the poor mechanical properties of the fish gelatin membranes, composite nanofibers made of fish gelatin and poly(L-lactide)(PLLA) were produced with a novel solution. The introduction of PLLA remarkably improved the mechanical properties of the gelatin membranes. With a combination of good biocompatibility and mechanical properties, fish gelatin/PLGA blending non-woven mats are considered to be very promising in fish fillet coating application.. in this study, we fabricated a novel nanofibers composed of fish collagen (FC) and polycaprolactone (PCL) blends by using the electrospinning method. Nanofibers were characterized using a scanning electron microscope (SEM), and it was revealed that the diameter of nanofibers decreases as FC content was increased in the FC/PCL composite nanofibers. Several modifications to the chitin NF surface are achieved, including acetylation, deacetylation and maleylation. The results of this study revealed that: 1–It is possible to produce Nanofibers from chitosan and fish gelatin. 2– Covering and coating of processed fish by nanofibers are applicable and increasing the possibility of shell life for the processed fish. 3– Nanofibers which have been produced from chitosan and fish gelation not only is environmentally friendly but also it will be eatable while has been covered for fish fillets. biocompatible chitosan and gelatin made from fish, fresh fish fillets do not have the ability to cover and packaging, but is edible and used.
    Keywords: Biology ; Chemistry ; Fisheries ; Iran ; Chitin ; Chitosan ; Nanofiber ; Chemical modification ; Fish ; Aquatic ; Maintenance
    Repository Name: AquaDocs
    Type: monograph
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  • 108
    Publication Date: 2021-07-16
    Description: In this study genetic characterization of Barbus brachycephalus caspius, Lucioperca lucioperca, Rutilus rutilus caspius, Rutilus frisi kutum and Salmo trutta caspius were examined by 611 samples from regions in East (Guilan province), Middle (Mazandaran province) and west (Golestan province) of southen part of the Caspian Sea. DNA was extracted from fin tissue by phenol -chlorophorm method and then PCR was performed using special primers. Statistical analysis of data was performed by Gene Alex, MEGA and Arlequin softwares. -Rutilus frisi kutum: The results showed that nine of ten primers were polymorphic loci. The mean of effective and observed alleles were 7.26±0.49 and 4.37±0.35 respectively. Also, the mean of expected and observed heterozygosity were 0.55±0.03 and 0.69±0.02 respectively Of the analysed loci, all of the samples (except Tajan and samples in LOC4 and Gilan samples in MFW2) possible tests were found to deviate significantly from the Hardy–Weinberg equilibrium (P〈0.05). The genetic diversity was significantly different between samples of Golestan and Gilan, Golestan and sefidrood, Golestan and Tajan, Mazandaran and sefidrood and Gilan and Tajan (p〈0.05). -Rutilus rutilus caspius: Sevan variable microsatellite loci were used to investigate genetic diversity and population structure of R. rutilus caspius. The mean of effective and observed alleles were 5.75±0.30 and 4.76±0.25 respectively. Also, the mean of expected and observed heterozygosity were 0.58±0.18 and 0.73±0.01 respectively. All of the samples (except golestan samples in LOC3) possible tests were found to deviate significantly from the Hardy–Weinberg equilibrium (P〈0.05) Of the analysed loci, the genetic divergence was significantly different between samples of Golestan and Gilan, Gilan and Mazandaran and Gilan with Gorgan bay (p〈0.05). -Salmo trutta caspius: Genetic characterization of S. trutta caspius was comparatively analyzed with mitochondrial DNA sequencing that 45 haplotypes was observed. The average of expected and observed heterozygosity were 0.61±0.35 and 0.33±0.12 respectively. The maximum of haplotype diversity (0.089±0.04) was in sardabrood river and the minimum was in Astara river (0.81±0.02). Also, the maximum of nucleotid diversity was 0.13±0.07 in Sardabrood and Chalos rivers and the minimum was 0.11±0.06 in Tonekabon river. In addition, the maximum and minimum of FST was 0.08 and 0.01 respectively. Of the analysed loci, the genetic divergence was significantly different between samples of Astara and Chalos, Astara and Tonekabon, Chalos and Karganrood and Tonekabon with Kaganrood (p〈0.05). - Barbus brachycephalus caspius: The size of amplified fragment was 800 bp in all of the samples. There were 24 variable loci and 12 haplotype that the maximum of haplotype was in Gilan area (8 haplotype). The average of expected and observed heterozygosity were 0.003±0.35 and 0.42±0.12 respectively. The results showed that the haplotype diversity was significantly different between samples of Sefidrood whit other samples (p〈0.05). In addition The maximum of nocleotid diversity was 0.005±0.003 in Sefidrood and minimum was 0.001± 0.001 in Tajan river. Of the analysed loci, the genetic divergence was significantly different between samples of Gilan and Tajan, Mazandaran and Sefidrood (p〈0.05). -Lucioperca lucioperca: The genetic diversity of L. lucioperca was analyzed by using microsatellite markers. Seven primer sequences available for were tested to amplify microsatellite loci that all of loci were polymorphic. The mean of effective and observed alleles were 6.14±0.45 and 3.88±0.34 respectively. Also, the mean of expected and observed heterozygosity were 0.662±0.03 and 0.70±0.02 respectively. The most of samples in PflaL6, PflaL7and PflaL8 loci possible tests were found to deviate significantly from the Hardy–Weinberg equilibrium (P〈0.05). The maximum of FST was 0.30 between Gilan and Mazandaran samples that there were minimum gene flow (8.18). The genetic divergence was significantly different between samples of Gilan and Mazandaran and Golestan whit mazandaran (p〈0.05).
    Keywords: Biology ; Iran ; Caspian Sea ; Genetic variation ; Barbus brachycephalus caspius ; Lucioperca lucioperca ; Rutilus rutilus caspius ; Rutilus frisi kutum ; Salmo trutta caspius ; Identification ; Population ; Genetic ; DNA ; DNA bank ; Molecular ; Microsatellites
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  • 109
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25716 | 18721 | 2018-10-14 03:15:28 | 25716 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Genetic knowledge helps to protect biodiversity and optimal harvest resources by several ways. This knowledge can help to reduce the risk of extinction to those populations that have high genetic diversity or diminished by detected them. Also, studies of population genetics can present guidelines for improving the structure of the population and understanding the biology of species. One of the most important applications of DNA database is detecting species, fishing offense, diagnose of anemia and genetic classification of animals. DNA bank has helped to identify the species that are hunted and were discovered only parts of their meat and texture. Optimal Storage of aquatic genetic resources and the conservation of biological diversity, as the national capital by using biotechnology methods are the most important goals of gene bank and database reserves of the country creation. Collecting biological specimens of aquatic species, preparation and long-term maintenance of them, genetic registration of endangered and threatened species and the use of biotechnology techniques for the protection, conservation and management of aquatic genetic resources is one of the other functions of the gene bank. Gene bank of inland waters of Iran (Gorgan) is planned and implemented to identify, collect, maintain and protect species at the level of genes, cells, tissues and live fish research and commercial exploitation. Despite the predicted projected funding for this project, unfortunately funding sources have been considered, does not allocated. Therefore some parts of the aims of this project has been organized by Golestan province credits to build molecular laboratory and aquaculture pond in Alang farm and exploitation of potential Gharasou station and Sijoval center restocking and the conditions for the bulk of the project goals is provided. Some parts like tissue culture laboratory and bioinformatics as well as parts and equipment related to the freezing of sperm and embryo development are the items that should be considered in the development of the this bank.
    Keywords: Biology ; Iran ; Inland Water ; GenBank ; Gene Bank ; Fish ; Species ; Genetic ; Biotechnology
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  • 110
    Publication Date: 2021-07-16
    Description: Goals: Determine of barcode of DNA in green tiger prawn, Penaeus semisulcatus, in the Gen bank of the species. Material and methods: In these study 30 specimens of Penaeus semisulcatus from each region in the Persian Gulf and Sea of Oman were sampled and preserved in ethanol 96%. The total DNA was extracted, COI gene was first amplified and then sequenced for each species. Finally the collected data were analyzed with the specific phylogenetic software. Result and discussion: Molecular analysis revealed some degree of interpopulation differences within two areas. Also for population study molecular data of species Penaeus semisulcatus were analysed base on COI RFLP and 16SrRNA sequences respectively. The results indicated that COI gen is a good marker for shrimp species differentiation that would be helpful to protect shrimp species.
    Keywords: Biology ; Iran ; Persian Gulf ; Oman Sea ; Genetic ; Molecular ; Population ; Cytochrome oxidized COI ; Penaeus semisulcatus
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  • 111
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25728 | 18721 | 2018-10-10 10:17:05 | 25728 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was conducted to determine growth parameters, catch and fishing effort trends, stock assessment and Acceptable Biological Catch (ABC) of bony fishes in the Iranian coastal waters of Caspian Sea in the years 2013-2014 and 2014-2015. According to the result, the numbers of beach seines were 124 and 120 and their fishing efforts were observed 44688 and 46299 beach seining, respectively. The total catches (including illegal fishing) were also obtained 17144.3 mt and 16733.2 mt during 2013-2014 and 2014-2015, respectively. The highest proportion of catch was belonged to kutum and golden grey mullet (94.4% and 89.5%, respectively) in two fishing seasons mentioned above. Growth parameters of kutum were estimated as K=0.19/yr, L_∞ =61.3 cm, t0=-0.99/yr. The Growth parameters were K=0.18/yr, L_∞= 61.1 cm, t0= -0.14/yr for golden gray mullet and were K=0.14/yr, L_∞= 70.8 cm, t0= 0/yr for common carp. Based on catch-at-age data, in the years 2013-2014 and 2014-2015, the total biomass, from the biomass-based cohort analysis were estimated 46900 mt and 41000 mt for kutum and 11550 mt and 12670 mt for golden grey mullet, respectively. The reference points of F0.1 and F35% were 0.41/yr and 0.34/yr for kutum and 0.36/yr and 0.33/yr for golden grey mullet, respectively. According to catch of species/groups, three main groups can be divided easily. The one–way ANOSIM catch similarity test indicated that coverage rates of different years were homologous and similar, but the coverage rates of different months, regions and periods were not similar. Also, there were significant differences in community composition of fishes in catch of beach seines among three times; three durations; three regions and seasons (in all cases MRPP, p〈0.001). During 1996-2000 species Rutilus rutilus, Vimba vimba, Chalcalburnus chalcoides, Aspius aspius, Barbus sp, Salmo trutta caspius and Silurus glani in the years 2000-2005 species/groups shads, Abramis brama and Esox lucius and in the years 2005-2012 species Cyprinus carpio and Stizostedion lucioperca were indicator species. Also, in the west region of the Caspian Sea species/groups Vimba vimba, Chalcalburnu schalcoides, Aspius aspius, Barbus sp, Abramis brama, Stizostedion lucioperca, Esox lucius and Silurus glani; in middle Rutilus frisii kutum, shads and Salmo trutta caspius and in east species/groups mullets, Rutilus rutilus and Cyprinus carpio were identified as indicator species. Based on available models, the ABCs were estimated as 8250-9750 mt for kutum and 1650-1950 mt for golden grey mullet (with precautionary approach 8250 mt and 1650 mt for kutum and mullet, respectively). For two species, the ABC with a lower and more accurate value based on more information, should be selected for the implementation of a precautionary management approach.
    Keywords: Biology ; Fisheries ; Iran ; Caspian Sea ; Bony fishes ; Growth ; Mortality ; Parameters ; Biomass ; Clustring ; Acceptable biological catch ; Cyprinus carpio ; Rutilus rutilus ; Salmo trutta caspius ; Rutilus frisii kutum ; Stizostedion lucioperca ; Abramis brama ; Esox lucius ; Aspius aspius ; Chalcalburnu schalcoides
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  • 112
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25726 | 18721 | 2018-10-10 10:00:38 | 25726 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Today, due to population increase and anthropogenic activities together with sewage and agricultural waste water entrance, aquatic ecosystems have been exposed to high pollutions. Phytoplankton is a group of water floating algae that have crucial roles in providing nutrients and oxygen for other organisms, nitrogen and CO_2 fixation. These organisms are considered as primary producers in aquatic ecosystems. They are found in various water habitats all over the world, affected by environmental variables such as pH, light and temperature and used for determination of water pollution degree and quality. Phytoplankton composition and density may be used as a complementary indicator of water trophy state. Phytoplankton communities indicate short and long term variations of aquatic systems. One of the most obvious problems in freshwater ecosystems is algal bloom or over growth of some blue- green algae which can decrease oxygen, and in some cases bring about toxin excretion and fish and human kills. In addition, many of bloom forming cyanobacteria produce secondary metabolites which can create sever poisoning in mammals including human. The genus Microcystis is a key bloom forming cyanobacteria in aquatic ecosystems. Populations of this genus form intense blooms in water bodies that has attracted more attentions in recent years. Various species of this alga have been distributed in stagnant and eutroph freshwater around the world. Microcystis has been defined by genetic criteria such as 16S rRNA molecular sequencing, but its classification in levels lower than genus is unclear and the presence of its classical morphospecies is doubtful. However, this genus creates sever blooms in eutrophic waters all over the world and many species produce toxins. Therefore, identification of its natural diversity in the levels lower than genus has high importance. However, several characteristics of Microcystis morphotypes which are classified a traditional species, actually are present and observed in different regions of the world. At present, they can be considered as morphospecies that belong to one genotype and have similar ecology. These traditional species with definite phenotypic and ecophysiological characteristics cannot be eliminated completely. Their identification is essential for ecological and ecotoxicological studies. Interistic transcribed separator (ITS) gene is a section of genome which is located between 16s rRNA and 23s genes. This gene has more heterogeneity than 16s rRNA; so it is used to identify many genera of cyanobacteria. The Aras reservoir located in the north-west of Iran plays important roles as fisheries, drinking and agricultural water supply and recreational activities in the region. The present thesis was undertaken to: analyze the Microcystis sp. populations by molecular methods such as ITS in Aras Reservoir. Samples for molecular analysis were collected from 10 sampling sites on 18 August 2013. Samples for molecular study of Microcystis were collected from two different depths (surface and 1 m depth) and transferred to laboratory without any treatments. Microscopic images of Microcystis were sent to professor Komárek and was confirmed.
    Keywords: Biology ; Iran ; Aras dam ; Molecular ; Barcoding ; Microcystis aeruginosa ; ITS-2 ; Gene ; Population ; Aquatic ; 16S rRNA
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25730 | 18721 | 2018-10-10 10:17:07 | 25730 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The main purpose of this study was survey on achieving recombinant DNA vaccine (live delivery) against streptococcusis for Rainbow trout (Onchorhynchus mykiss) Immunization. Initially, a total of 515 samples were collected from the head kidney of diseased fish (weigh 50_ 200g) in 72 farms of 8 provinces. Approximately, 40% (206 samples) of specimens were infected to Streptococcus species. Then were isolated 172 DNA samples and consequently, five pathogenic species have been identified, including S. iniae, S. faecium, S. agalactiae, S. dysgalatiae, and S. uberis. The enzyme phosphoglucomutase (PGM) has recently been discovered to play an important role in polysaccharide capsule production and virulence in S. iniae. Therefore, was initially isolated S. iniae and cloning phosphoglucomutase gene. Then, the PGM gene was amplified successfully and cloned in pTZ57R cloning vector. The recombinant plasmid was sub cloned into pETD uet-l expression vector by restriction enzymes and confirmed by PCR. Meanwhile, for amplifying simA and cpsD genes were used universal primers pNZ8148 and special for simA and cpsD genes.The recombinant bacteria Lactococcus lactis (NZ9000) was used for transformation the plasmid into Lactococcus lactis. Vaccination was performed by oral, bath and injection (peritoneal) methods. The efficiency of g2 was better than g1in these three methods and all of groups. The determination of IgM level, or detection of anti S. iniae antibody was carried out by using ELISA. The results revealed that there was a significant (p〈0.05) difference between the level of IgM in all three methods and experiment groups compare to control group. The results of challenge of vaccinated fish with S. iniae showed that fish RPS in all of groups except group 3 (42.63 percent) were more than 50 percent while, in control group was 21.43 percent. The highest fish RPS was belong to group 11(61.25 percent) and statistical analyses revealed that significant (p〈0.05) difference between fish vaccinated RPS, compare to control group.
    Keywords: Biology ; Health ; Iran ; Survey ; DNA ; Live delivery ; Rainbow trout ; Onchorhynchus mykiss ; Immunization
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25736 | 18721 | 2018-10-13 06:14:26 | 25736 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This project prepared and implemented during 2013-16 with emphasis on to provide the results of the fish larva projects (identification, abundance and distribution) in the Iranian waters of the Persian Gulf and Gulf of Oman which as well as had already been done by Iranian Fisheries Science Research Institute and affiliated research institutes and centers . Since each marine fish stocks management requires increased knowledge of the life cycle and be complete biological information, obviously implementation and increased field research is essential, on the other hand the results and the availability of data and information to operation and coordination with the scientific community at national and international levels is of particular importance. This is on condition that currently, due to the increasing trend researchers and students Aquatic Sciences and Fisheries, the availability of data resulting from studies and research projects and initiatives will be necessary. The site is currently only in view of identification and distribution of marine fish larva has been prepared in Farsi with photo gallery, and English language development and preparation of the site for the international operation there. It is worth mentioning in the same areas and the same site adjacent seas of fish larvae has not been prepared and is not available. So this site can also be considered as reference area. This site is available for researchers, scientist and students as address: http://ifl.ifro.ir/
    Keywords: Biology ; Information Management ; Iran ; Database ; Larvae ; Fish ; Net work
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25746 | 18721 | 2018-10-13 06:11:23 | 25746 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: At present, the aquaculture industry to provide proper instructions in the field of health management, including production of Specific Pathogen Free shrimp (SPF), require sensitive and reliable methods for the detection and identification of pathogenic microorganisms. Molecular methods which used in the detection of microorganisms have a high discriminatory power in the taxonomy and in relation to libraries in the world. On the other hand, the accurate identification of microorganisms, providing the genetic data bank of shrimp pathogens and maintenance of these strains is the step to promote further research on the mechanisms of pathogenesis of pathogens, diagnosis, treatment, prevention of disease, identify indigenous production kits, diagnosis re emerging and emerging diseases and their origin. Therefore, in this project, by using ribotyping technique, native isolated pathogenic bacteria and fungi were identified and recorded in the gene bank database center. During sampling of shrimp and water of Specific Pathogen Free shrimp center, 40 bacterial strains were isolated, which 8 of them had the most frequency and identification based on 16S rDNA sequencing was performed. Bacteria identified are: Vibrio nigripulchritudo strain IS013(GenBank:KP843725), Vibrio brasiliensis strain IS014 (GenBank:KR186076), Vibrio rotiferianus strain IS015 (GenBank:KR186077), Vibrio azureus strain IS012 (GenBank:KJ018724.1), Vibrio owensii strain IS016 (GenBank:KR186078), Agarivorans gilvus strain IS017 (GenBank:KR186079), Vibrio brasiliensis IS018 (GenBank:KR186080) and Vibrio alginolyticus strain IS019 (GenBank:1817854), which were recorded in The World Bank genes. In this study fungal isolates were not detected.
    Keywords: Biology ; Iran ; Bacteria ; Fungi ; Ribotyping ; 16S rDNA ; Shrimp ; Specific pathogen free ; Vibrio rotiferianus ; Vibrio owensii ; Vibrio brasiliensis ; Vibrio azureus ; Agarivorans gilvus ; Vibrio alginolyticus ; GenBank
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25749 | 18721 | 2018-10-13 07:31:35 | 25749 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: After the outbreak of various viral diseases, specifically white spot syndrome (WSS) in farmed Litopenaeus vannamei in the past few years, this non-native shrimp species in Iran requires a generator-builder with specific a pathogen-free approach. This Research was done by the Iran Shrimp Research Center (ISRC) to access accurate information regarding specific pathogen-free shrimp production and avoid using shrimp broodstock production through foreign investors. This research was part of the molecular viral study. For this trial, selected shrimps in farms were sampled and screened for main viruses (OIE list). Shrimps without infection were brought to the quarantine system for one month and at the end of the quarantine they were screened again. In addition, in winter spending, before and after spawning, it’s offspring in F1 and F2 were also screened for viral pathogens by IQ2000 kit at the Iran Shrimp Research Center lab and Iranian veterinary organization lab. All shrimp products such as fresh and consumed food were tested for OIE list at all stages during project performance and the result of tests were negative. Sequenced and molecular tests with specific primers were used to determine the presence of infected samples carrying the virus in any of the products, such as shrimp and consumed food, the infected samples. The result of all the tests during last three years, shows that all the shrimp and their food products were pathogen-free and safe to use.
    Keywords: Biology ; Iran ; Shrim ; SPF ; Virus ; White Spot Syndrome Virus ; WSS ; Spawning ; Molecular
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25747 | 18721 | 2018-10-13 07:20:06 | 25747 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Protection authorities of plant and animal genetic resources and conservation of biodiversity as national assets by using biotechnology techniques is the most important objectives of genetic data information bank. Establishment of shrimp species genetic data bank, makes possibility of study multiple genes such as economic genes and since knowledge of genetic variation within and among populations and barcoding species of shrimp are very important for species conservation and with so little researches of Asia in the global studies of DNA barcoding and animal taxonomy in Iran, In this study, Iran shrimp genetic diversity and genetic data bank was done. DNA barcode is a short, standard well known sequence of cytochrome oxidase І gene. By using this DNA sequence can be realized that each animal, plant or fungus belongs to which species. Also prepare a bank of genetic data shrimp pathogens and maintenance of these strains are steps to advance future researches in the fields of pathogenesis mechanism of pathogens, diagnosis, treatment, Disease prevention, production diagnosis native kits and detection of emerging and reemerging diseases its origins. So in this plan samples were collected from imported cultured shrimp Litopenaeus vannamei and and 6 Persian Gulf and Oman Sea shrimp species which classified based on traditional systematically as: Penaeus semisulcatus, Fenneropenaeus merguiensis, Metapenaeus affinis, Parapenaeopsis Stylifera and Fenneropenaeus indicus. After examination of DNA barcode sequence, molecular and bioinformatics operations of each sequence in the Consortium for the Barcode of Life (CBOL) and National Center for Biotechnology Information (NCBI), phylogenetic analysis of each sample was determined and similarity of each sample with NCBI and CBOL database was checked and the closest species to each sample were specified. According to the results different samples of L. vannamei, . banded P.semisulcatus, F. merguiensis and F. indicus have more than 97% similarity to the same species of other countries. non banded P.semisulcatus had 80.07% similarity to banded P.semisulcatus, M. affinis samples had 90.3% similarity to Metapenaeus ensis and Parap. Stylifera had 93.44% similarity to Parapenaeopsis coromandelica in the CBOL. This funding confirmed the need for further investigation and possible announcement of new species. By using ribotyping technique, native isolated pathogenic bacteria and fungi were identified and recorded in the gene bank database center. During sampling of shrimp and water of Specific Pathogen Free shrimp center, 40 bacterial strains were isolated, which 8 of them had the most frequency and identification based on 16S rDNA sequencing was performed. Bacteria identified are: Vibrio nigripulchritudo strain IS013(GenBank:KP843725) ،Vibrio brasiliensis strain IS014 (GenBank:KR186076) ،Vibrio rotiferianus strain IS015 (GenBank:KR186077) ،Vibrio azureus strain IS012 (GenBank:KJ018724.1) ،Vibrio owensii strain IS016 (GenBank:KR186078) ،Agarivorans gilvus strain IS017 (GenBank:KR186079) ،Vibrio brasiliensis IS018 (GenBank:KR186080) and Vibrio alginolyticus strain IS019 (GenBank:1817854), which were recorded in The World Bank genes. In this study fungal isolates were not detected.
    Keywords: Biology ; Iran ; Persian Gulf ; Oman Sea ; Bacteria ; Ribotyping ; 16S rDNA ; Shrimp ; DNA barcode ; GenBank ; Vibrio nigripulchritudo ; Vibrio rotiferianus ; Vibrio owensii ; Vibrio brasiliensis ; Vibrio azureus ; Agarivorans gilvus ; Vibrio alginolyticus ; Parapenaeopsis coromandelica ; Metapenaeus ensis ; Litopenaeus vannamei
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25750 | 18721 | 2018-10-13 07:33:30 | 25750 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: DNA barcode is a short, standard well known sequence of cytochrome oxidase І gene. By using this DNA sequence can be realized that each animal, plant or fungus belongs to which species. in this research, samples were collected from imported cultured shrimp Litopenaeus vannamei and and 6 Persian Gulf and Oman Sea shrimp species which classified based on traditional systematically as: Penaeus semisulcatus, Fenneropenaeus merguiensis, Metapenaeus affinis, Parapenaeopsis Stylifera and Fenneropenaeus indicus. After examination of DNA barcode sequence, molecular and bioinformatics operations of each sequence in the Consortium for the Barcode of Life (CBOL) and National Center for Biotechnology Information (NCBI), phylogenetic analysis of each sample was determined and similarity of each sample with NCBI and CBOL database was checked and the closest species to each sample were specified. According to the results different samples of L. vannamei, . banded P.semisulcatus, F. merguiensis and F. indicus have more than 97% similarity to the same species of other countries. non banded P.semisulcatus had 80.07% similarity to banded P.semisulcatus, M. affinis samples had 90.3% similarity to Metapenaeus ensis and Parap. Stylifera had 93.44% similarity to Parapenaeopsis coromandelica in the CBOL. This funding confirmed the need for further investigation and possible announcement of new species.
    Keywords: Biology ; Information Management ; Iran ; DNA barcode ; Shrimp ; Litopenaeus vannamei ; Penaeus semisulcatus ; Fenneropenaeus merguiensis ; Metapenaeus affinis ; Parapenaeopsis stylifera ; Fenneropenaeus indicus
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    Publication Date: 2021-07-16
    Description: This study was conducted to determine phosphorous and nitrogen species at the surface sediment in the southern Caspian Sea (Mazandaran Coast, Kelarabad). Surface sediments samples were collected at three stations from autumn of 2011 to winter of 2012. All samples were prepared by digesting and extracting processes and then phosphorous and nitrogen species concentrations determined using spectroscopy instrument. Results of this study showed that annual mean of Loosely-P, Fe-P, Al-P, Bioava.-P, Ca-P, TIP, Rese.-P and TP was obtained as 5.06±0.33, 55.11±2.52, 42.38±3.74, 102.52±5.68, 172.91±7.12, 275±9.12, 333.30±28.52 and 608±52 µg/g.dw, respectively. The results also showed that inorganic phophorous was less than organic phophorous during different seasons, however, mean percentage of residue-P containing organic compounds and non-degradable compounds was more than 50 percent. Percentage of Ca-P was higher than 60, whereas Bioava.-P was less than 40 percent. In addition, Fe-P and Loosely-P attained the maximum and minimum values, respectively, among the bioavailable phophorous. The order of different forms of phosphorous were recorded as Org-P〉Ca-P〉FeP〉Al-P〉Loosely-P. Annual mean of NH4/N, NO2/N, NO3/N, TIN, TON and TN were observed as 4.23±0.50, 0.06±0.01, 0.74±0.12, 5.02±0.53, 2.48±0.63 and 7.53±0.51 µg/g.dw, respectively. Annual percentage of TIN was two folds than TON and concentration of NH4/N was also four times than NO3/N. As a conclusion, the results revealed that main causes of Bioava.-P adsorption and desorption were temperature, Eh and pH. Also, the form of NH4+/N was of a high percentage because of anaerobic condition in the sediments. According to the high ratios of nitrogen/phosphorous of sediments to nitrogen and phosphorous of bottom water, it finds that released of those from the sediments to water will be happened with high rates. Therefore, it is expected that the establishment of fish farming cages should be carried out with more precautionary approaches which not leads to increased algae bloom.
    Keywords: Biology ; Chemistry ; Ecology ; Iran ; Caspian Sea ; Kelarabad ; Mazandaran coast ; Phophorous ; Nitrogen ; Surface sediment ; Determination ; Breeding
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25763 | 18721 | 2018-10-13 07:32:19 | 25763 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The aim of this study was to determine the genetic parameters, genetic differentiation and relative relationship between different generations of specific pathogenic free of L.vannamei via molecular methods. Tissue samples of shrimp were randomly get caught from the different area. Genetic parameters were evaluated by 12 special primers of L.vannamei via microsatellite assay. In this study, two population of Molokaei and High health were identified as shrimp broodstocks of specific disease free (SPF) zero generation. Breeding among shrimp (males and females) were through inbreeding and crossbreeding. Three various stocks were produced: H♂ × M♀, M♂ × H♀ and H♀ × H♂. Genetic differences and relative relationship between different generations of specific pathogenic free shrimp were to evaluate by evaluation of 16S rRNA and d-loop region mitochondrial of different generations, respectively. The results showed that significant differences were observed among genetic distance of different populations of L.vannamei farmed (0.615). There were o.o78 Fst value of M.H and H.M stokes that it was a significant difference (P 〈0.001). The analysis of d-loop region of mitochondrial genomes of different generations shrimp was observed 997 positions with 3-6 and 766-799 of haplotype and monomorphic, respectively. Despite haplotype diversity and nucleotide diversity of all generations SPF shrimp were 0.877 and 0.12, respectively. While, Base on result of 16S rRNA indicated that of 486 sites have been identified 484 sites were conserved. Also, monomorphic sites ranged between 482-486 sites and was consists 2 polymorphic sites and 2 transitional sites. The number haplotypes, haplotypes diversity, nucleotide diversity revealed in this region were 2, 0.356±0.159 and 0.00147, respectively. Regarding the results of in this study can be concluded that lack of arriving new broodstocks from outside the country and was mating between adult males and females relative (full-sib) addition to small effective population size there was both genetic drift and number of haplotypes was gradually reduced from zero generation to second generation. Hence, due to the high close relationship between different generations shrimp evaluation of d-loop and 16S rRNA regions of the mitochondrial genome were little genetic difference between them.
    Keywords: Biology ; Iran ; Litopenaeus vannamei ; Genetic parameters ; Microsatellite ; Mitochondrial genome ; 16S rRNA ; 16S rRNA region ; Evaluation ; Detection
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25767 | 18721 | 2018-10-13 08:03:37 | 25767 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The sex reversal technique in fish is widespread in many countries. The development of these techniques is desirable because rainbow trout males reach their gonad maturity earlier compared to the females. Rainbow trout alevins were treated with 17α-methyltestosterone incorporated in the diet (0.5. 1.5, 3, 6 and 10 mg/kg) for 60 days from the beginning of first feeding. Sex was determined at 180 and 680 dpf by sampling fish (n = 20) from each group and examining gross gonadal morphology under a dissecting microscope. Also sex reversal ratio and growth performance were determined in hormone-treated groups. These sex reversed functional males were reared for brood stock until they attained sexual maturity. At the end of experiment, normal rainbow trout eggs were fertilized with the sperms taken from sex reversed males for producing all-female populations. Examination of the results showed that 17α- methyltestosterone was effective in all treatment. The highest sex reversal ratio with 100% was observed in group treated with 0.5, 1.5 and 3 mg/kg 17α- methyltestosterone. The highest live weight ratios were observed in groups fed with 6, 0.5 17α-methyltestosterone and control group. Female progeny produced from the sex reversed males were 100 % in all males that sired offspring. All female trout stocks produced by this method have advantage in rainbow trout culture since the fish is not meant for direct human consumption and is used to generate brood stock, therefore, difference of growth parameters do not influence the success.
    Keywords: Aquaculture ; Biology ; Iran ; Sex differentiation ; Oncorhynchus mykiss ; Sex reversal ; Hormone treatment ; 17-αmethyltestosterone ; Female ; Population ; Steroids ; Gonad ; Maturity ; Rainbow trout ; Sexual
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25764 | 18721 | 2018-10-13 08:00:04 | 25764 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The report provides comprehensive information and exclusive native species and their status in terms of protection, browse and view Resorts® reserve forest vegetation and coastal areas of the Persian Gulf and Oman Sea and Caspian Sea is mentioned. In a series of studies including field trips, collect and identify plants in Iran, the list has been presented since 1362 to date in the form of plans and programs identify plants is planned. It should be noted that all identified studies and knowledge of plants and notes as well as an identification key based on the information contained in Flora Iranica valuable book written K.H. Rechinger plays a key role and is essential to identify the plants. along with scientific names, synonyms and names mentioned Farsi and English with the geographic distribution is presented according to two area Shmnaly and south. Based on existing resources, conservation status of species under national legislation and international and native status (Endemic) and biological values and biodiversity of geographically referenced.
    Keywords: Biology ; Ecology ; Iran ; Fauna ; Flora ; Coastal Zone ; Community
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25769 | 18721 | 2018-10-13 08:05:39 | 25769 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study, after determining history of origin entering white leg shrimp of broodstocks at hatcheries of country, finally, two population of Molokaei and High health were considered as shrimp broodstocks of specific disease free (SPF) zero generation. After inbreeding and cross breeding among adult males and females of two population above, three storage different were produced in the first generation: H♂ × M♀, M♂ × H♀ and H♀ × H♂. The second generation were produced from selective breeding of M♂ × H♀. The aim of this study was to determine genetic differences between different generations of specific pathogenic free shrimp by evaluation of 16S rRNA region mitochondrial of different generations. Result of this study showed that of 486 sites have been identified 484 sites were conserved. Also, monomorphic sites ranged between 482 - 486 sites and was consists 2 polymorphic sites and 2 transitional sites. The number haplotypes, haplotypes diversity, nucleotide diversity revealed in this region were 2, 0.356±0.159 and 0.00147, respectively. Due to the high genetic identify and reduce of genetic distance of shrimp between different generations of SPF shrimp, genetic differentiation and gene flow (Nm) between them were -.142 and -2.00, respectively. This rates clearly shows a low level of polymorphism in this region of mitochondrial of different generation of SPF. This may be occurred because of small effective populations and genetic drift. Thus, offspring had inherited mitochondrial genome from your mother, therefore, it is expected that rate of variation in this genome is influenced by several factors, including genetic drift, due to unavailability of new-broodstock from different areas. It was observed that due to high conservation sites in 16S rRNA region of mitochondrial genome, nothing genetic differentiation was observed among different generations of SPF.
    Keywords: Aquaculture ; Biology ; Iran ; Litopenaeus vannamei ; Mitochondrial genome ; 16S rRNA ; Genetic differentiation
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25770 | 18721 | 2018-10-13 07:51:12 | 25770 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study focused on population structure, biodiversity, and abundance of the Chitgar Lake fishes in October 2014. Based on the lake location fishes samples were collected at 34 stations in water body. In this study identified 8 family belong to 18 species comprised of Cyprinidae (11 species), Cichlidae (one species), Loricariidae (one species), Pangasiidae (one species), Serrasalmidae (one species), Poeciliidea (one species), Salmonidae (one species), and Scaridae (one species) were Identified. Among the fishes just one native fish from Capoeta bohsei species observed during the study. The dominant lake fishes were invasive species Hemiculter leucisculus, Alburnus hohenackeri, Carassius auratus, Carassius gibelio and Pseudorasbora parva. The highest abundance of fishes were H. leucisculus 62 % and A. hohenackeri 35 % by Seine net. Furthermore, those were dominant abundance (69 and 12 % respectively) in Gill net method. Finally in Cast net method A. hohenackeri 38 %, Pseudorasbora parva 21 % and H. leucisculus 17 % were dominated in the shore region. The lowest fishes abundance were P. hypophthalmus and Parrotfish. Overall more than 90 % of the fishes population were invasive and alien species. Thus, the present of invasive fishes and predator fishes luck in the Chitgar lake might be reason for ecosystem ruin, algae bloom, feed competitive, fish disease and trophy level rises.
    Keywords: Biology ; Ecology ; Iran ; Fish ; Biodiversity ; Species ; Abundance ; Cyprinidae ; Cichlidae ; Loricariidae ; Pangasiidae ; Serrasalmidae ; Poeciliidea ; Salmonidae ; Scaridae ; Capoeta bohsei ; Hemiculter leucisculus ; Alburnus hohenackeri ; Carassius auratus ; Carassius gibelio ; Pseudorasbora parva
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25772 | 18721 | 2018-10-13 08:08:05 | 25772 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Seaweed belonged to baseborn marine plants with cell wall containing of valued polysaccharides such as Agar, Alginate, Limen sulfate and Carmine, used as food additives and in different industries. Base on previous studies, more than 1000 MT. of Sargassum seaweed withdraw from Oman Sea in Sistan and Baluchistan coastal line which can be collect, dried and powdered for using as supplement in shrimp feed. In this project, Sargassum ilicifolium collected from 6 coastal areas, rinsed, dried, powdered and measured the nutritional values in laboratory for surveying statistically. According to the high nutritional value of Tis coastal seaweed, this variety seaweed powder, replaced with protein resources (fish meal and Soy and Wheat) of white-leg shrimp feed which was formulated by Havorash feed factory of Boshehr in four treatments (A: as control without any replacement) B: with 5%, C: 10 % and D: 15% seaweed replacement, each with three replicates in order to obtain isonitrogenus 33% CP., and iso-caloric (13% fat and 15% carbohydrate) feed using winfeed software. The weighed milled ingredients were carefully mixed using a laboratory food mixer. The mixtures were primed with 30% hot water to yield a suitable pulp. Wet diets were made into 2 mm pellet size and dried at 40 °C in a drying cabinet and maintained in standard condition which was used for water stability and absorption capacity test of the pellets in sea water, statistically one way- ANOVA. The Tis coastal seaweed with 9.8% CP, 2% lipid and 23% carbohydrate had higher nutritional value compared to the other gathered seaweed. Also amino acid and fatty acid profiles, vitamins and minerals were measured in all seaweed samples each, with three replications. As result, for using Sargassum ilicifolium as vitamins and mineral premixes in white- leg shrimp feed, Zinc, Cobalt and Phenylalanine with dose of 1.1, 06.0 and 4.0 ml. 100% dried seaweed must be added respectively. The water stability of D feed treatment in seawater (98%) and C (97%) had statistical differences with A and B (95% stability) (P〈0.05). Water absorption capacity of feeds after one hour immersion in seawater showed significance difference between D (110%) and three others, C(100%), B(85% and control(80%) (P〈0.05). As final aim of this project, enriched product of this seaweed as minerals and vitamins supplements were handsel joinery in the Second Medicine Plant Festival, 2016 and registered in recording organization to give the final certificate.
    Keywords: Aquaculture ; Chemistry ; Iran ; Sargassum illicifolium ; Sargassum illicifolium ; Mineral and vitamins supplements ; Natural binder ; Litopenaeus vannamei ; Seaweed ; Vitamin ; White leg shrimp
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25775 | 18721 | 2018-10-13 08:12:07 | 25775 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Present study carried out between September 2011 and October 2012 in 19 sampling sites in order to investigate the trophy level, productivity, and natural dominant living conditions in the Gorgan Bay. According to the provided maps from the shoreline, depth, and sediment, the total area and volume of Gorgan Bay is 466 square meters and 905.33 million cubic meters respectively. Also the physic-chemical parameters including: water temperature, EC, salinity, transparency, DO, BOD_5, pH, Ammonia, Nitrate, Total hardness, Total alkalinity, and phosphate are determined and studies. 3 main phylum, 12 orders, 6 classes, and 12 families from bottom living organisms (macro-benthos) were identified in this region. Standard deviation in TSI was from minimum 37 in March up to maximum 65 in September and the annual average was 53. According to the average TSI in different months, for five months namely as March, April, June, August, and February the dominant situation was eutrophy. For the rest of months namely as May, September, October, November, December, and January the dominant situation was Meso-trophic in the Bay. Based on calculation, minimum and maximum productivity in the Bay were 92.26 and 700.66 Kg per hectare in October and July respectively. The annual average of productivity was 195.10 Kg per hectare.
    Keywords: Biology ; Ecology ; Iran ; Gorgan Bay ; Trophy level ; Productivity ; Salinity ; Physic-chemical parameters
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25777 | 18721 | 2018-10-13 08:20:28 | 25777 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The aim of this study was stablish of gene bank on The Caspian Sea bony fish, identification genetic characterization and population of Barbus brachycephalus caspius, Lucioperca lucioperca, Rutilus rutilus caspius, Rutilus frisi kutum and Salmo trutta caspius in southern part of the Caspian Sea by molecular method (Microsatellites) and formation DNA bank, create of cryopreservation bank of bony fish and the feasibility of creating a natural habitat for spawning of bony fish in the Caspian Sea in order to preserve biodiversity and genetic risk. The results showed that The genetic diversity was significantly different between samples of Golestan (Goegan Gulf), Gilan (Sefidrood, Karganrood and Astara rivers) and Mazandaran (Tajan, Tonekabon, Sardabrood and Chaloos rivers) (p〈0.05) and there were different genetic groups. In addition, DNA bank of these species was carried out. After sperm sampling of Rutilus frisi kutum and Salmo trutta caspius, their quality were tested and some parameters such as motility, duration of mobility, density, pH and osmolality were measured. The results showed that, percentage of motile sperm, timing motility and sperm density of Caspian trout were higher than those of Caspian kutum but osmolality and pH of Caspian trout were lower than those of Caspian kutum. Over time, the percentage of sperm motility and mobility for both species declined compared with fresh samples. After thawing, percentage of motile sperm and timing motility of Caspian kutum were lower than those factors Caspian trout. The results showed that the sample of Caspian kutum sperm that were diluted by ethylene glycol after thawing and were immotile all of them. However, the samples were diluted by glycerol, after thawing, were alive and motile. According to the results, it seems very important species differences that must be fully considered in the process of freezing sperm. The use of a single protocol would not be successful in cryopreservation because the reaction of sperm against to chemical agents is variable. Sampling of physical and chemical parameters of water, macrobenthic invertebrates and fish fauna was conducted seasonally in each rivers (Tajan, Haraz, Sardabrood and Chaloos rivers) at selected stations during one year. A total of 53 taxa of macrobentic invertabrates were identified in the four mentioned rivers. 15 species of fish were determined in the studied rivers that 6 of them were non-native species. All identified fish species were native and the anadermous fish was not catch. The results showed that, the relative abundance of fish species resistant to pollution and hard environmental conditions were much more that sensitive species. Physicochemical parameters of water, turbidity, nutrients, biodiversity, nutrition functioning group and present or absent of fish species showed that there are significant decrease in water qualities from upstream ratio to downstream and there is not any possibility to natural reproduction of migrated species that are dominant and in danger at downstream. In Shahid- Rajaei dam the maximum relative abundance of fish species belonged to Capoeta capoeta, Luciobarbus capito and Squalius cephalus, respectively. The resurvey of this dam and its upstream have been preparing such a favorable condition that seems there are potential of introduction and inhabitant of some of the in danger fish species as a genetic reserve for biodiversity preservation.
    Keywords: Biology ; Iran ; Caspian Sea ; Shahid-Rajaei dam ; Genetic variation ; Gene bank ; Freezing sperm ; Natural reproduction ; Squalius cephalus ; Luciobarbus capito ; Capoeta capoeta ; Barbus brachycephalus caspius ; Lucioperca lucioperca ; Rutilus rutilus caspius ; Rutilus frisi kutum ; Salmo trutta caspius
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25778 | 18721 | 2018-10-13 08:00:35 | 25778 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study aimed to identify of helminth parasites in some native and economic fish of Anzali wetland was performed. 314 fish specimens, including: Alburnus hohenackeri (60 Specimen), Blicca bjoerkna (75 Specimen), Hemiculter leucisculus (75 Specimen), Pseudorasbora parva (52 pcs) Rhodeus amarus (52 Specimen), from Cyprinidae (Cyprinidae), during 1 year (2012-2013) and seasonally, from three different stations (Shijan, Pirbazar and Pasikhan) via gill net (50-70 mm) and fyke net were sampled. Samples were transported to the Inlandwater Aquaculture Institute in Bandar Anzali. In the laboratory, After biometry and determine the age and sex of fish, different body parts for searching of parasites were checked and parasites by using identification keys were identified. As a result of this study, 9 species of helminth parasites of fishes were identified include: Gyrodactylus sp., Dactylogyrus sphyrna , Dactylogyrus sp., Ligula intestinalis, Diplostomum paraspathaceum, Diplostomum spathaceum, Diplostomum sp., Posthodiplostomum cuticola and Rhabdochona denudate. The highest frequency of parasites in trematodes (in 5 fish species studied) and the minimum frequency in cestodes (each at 1 fish species studied) were seen. According to statistical analysis using Chi-Square test and KruskalWallis, the pollution of the sex, weight, length and sampling stations, there is no statistically significant difference (P〉 0.05).
    Keywords: Biology ; Health ; Iran ; Anzali wetland ; Fish ; Helminth parasites ; Blicca bjoerkna ; Hemiculter leucisculus ; Pseudorasbora parva ; Rhodeus amarus ; Dactylogyrus sphyrna ; Dactylogyrus sp ; Ligula intestinalis ; Diplostomum paraspathaceum ; Diplostomum spathaceum ; Diplostomum sp. ; Posthodiplostomum cuticola ; Rhabdochona denudate
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25779 | 18721 | 2018-10-13 08:34:12 | 25779 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Genetic studies and gene banks preparation can identify guidelines for improving diversity and population structure and estimation, poaching and also the amount of cross breeding and provide genetic classification. In this study, sampling was performed from the important studied species habitats areas for the species such as: Dasyatis bennetti، Netuma thalassina, Netuma bilineata Carcharhinus leucas، Choerodon robustus، Pseudorhombus pentophthalmus ،Pseudorhombus arsius Thunnus tonggol ،Euthynnus affinis and Trichiurus lepturus. Total DNA extraction was performed using phenol - chloroform method which is the most common method for DNA extraction in order to achieve high quality of DNA was performed in the preparation of gene bank in this study. After relevant studies on this gene primers were designed and in use. After editing the sequences, nucleotide BLAST (Basic Local Alignment Search Tool) was performed using NCBI blast main page. The sequences obtained from each sample were aligned and corrected from any ambiguities and assembled using Bio edit program .Trees were generated using maximum parsimony (MP), a character-based algorithm and neighbor joining (NJ) a distance-based algorithm for phenetic analysis. The distance matrix option of MEGA4 was used to calculate genetic distance according to the Kimura 2-parameter model of sequence evolution. Based on the results obtained, the optical density of 260 to 280 nm in the samples was recorded between 1/8 - 2, indicating good quality DNA samples. Optimized PCR reaction to 16SrRNA gene amplification using the gradient between 48 - 60° C showed that the most suitable criteria for binding primers, 54 to 58 Celsius degrees respectively. The project objectives including the identification of the genetic structure of the species, and draw the phylogenetic trees using two genes 16SrRNA, making identification and registration of specified computer storage and regulate the structure and management of mentioned species by focus on genetic resources 10 species of majour commercial and noncommercial fishes in the Persia Gulf and Oman Sea through the creation of an integrated network of aquatic genetic resources in the region to try to identify genetic resources and aquatic gene bank. This type of analysis could be considered as an important tool to be used in broodstock selection in breeding programs. Also identify the species of fish named as Pseudorhombus pentophthalmus with a new haplotype and genetic differences 7% in compare to the samples recorded in the World gene Bank can considered as a major achievements of this research.
    Keywords: Biology ; Iran ; Persian Gulf ; Gene Bank ; mtDNA ; Fish ; Marine ; 16S rRNA ; Dasyatis bennetti ; Netuma thalassina ; Netuma bilineata ; Carcharhinus leucas ; Choerodon robustus ; Pseudorhombus pentophthalmus ; Pseudorhombus arsius ; Thunnus tonggol ; Euthynnus affinis ; Trichiurus lepturus ; Species
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25785 | 18721 | 2018-10-13 08:05:51 | 25785 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Genetic knowledge and Gene bank preparation can help to protect biodiversity and detect , species identify , fishing offenses , genetic classification and also identification the faliure cross hybridizations of marine animals. In this study, sampling was performed from Jask, guatr and Hormuz areas, which is the most important habitats for the species studied using bottom trawl. Total DNA extraction was performed using phenol- chloroform method. After relevant studies on this gene primers were designed and in use. After editing the sequences, nucleotide BLAST (Basic Local Alignment Search Tool) was performed using NCBI blast main page. The sequences obtained from each sample were aligned and corrected from any ambiguities and assembled using Bio edit program. Trees were generated using maximum parsimony (MP), a character-based algorithm and neighbor joining (NJ) a distance-based algorithm for phenetic analysis. The distance matrix option of MEGA4 was used to calculate genetic distance according to the Kimura 2-parameter model of sequence evolution. Based on the results obtained, the optical density of 260 to 280 nm in the samples was recorded between 1/8 - 2, indicating good quality DNA samples. Optimized PCR reaction to 16SrRNA and COI gene amplification using the gradient between 48 - 60° C showed that the most suitable criteria for binding primers, 48 and 54 Celsius degrees respectively. The project objectives including the identification of the genetic structure of the species, and draw the phylogenetic trees using two genes 16SrRNA and COI, making identification and registration of specified computer storage and regulate the structure and management of mentioned species by focus on genetic resources 5 species of shrimp (P. semisulcatus، P. indicus، P. merguiensis ، P. monodon، M. affinis) in the Persia Gulf and Oman Sea through the creation of an integrated network of aquatic genetic resources in the region to try to identify genetic resources and aquatic gene bank. Molecular investigation of mitochondria DNA (mtDNA) using partial sequences of 16S rRNA gene showed relatively low genetic differences between the P. semisulcatus morphotypes. These sequences were able to distinguish between the two morphotypes, and separated them into two distinct clades. Also genetic divergence detected by COI gene analysis was consistently higher. High genetic divergence for COI was observed between the two morphotypes of P.semeisulcatus which emphesise that the gene bank preparation should be perform for this morphotype of this species. This type of analysis could be considered as an important tool to be used in broodstock selection in breeding programs. In this case, different management in broodstocking programs should be performed for two morphotypes of P. semisulcatus which were detected in Persian Gulf. The results of this study show that two Morphotype of P.semisulcatus can be considered as two separate species from genetic aspects. In this regard, it can be assumed that the genetic composition of the studied species is very close together and we've no seen a huge difference in the species except in the green tiger species.
    Keywords: Aquaculture ; Biology ; Iran ; Persian Gulf ; Oman Sea ; Gene Bank ; mtDNA ; Genetic Resourcess ; 16S rRNA ; Cytochrom Oxidase I ; P. semisulcatus ; P. indicus ; P. merguiensis ; P. monodon ; M. affinis ; Genetic
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    Publication Date: 2021-07-16
    Description: Concentration of chlorophyll-a and quantitative feature of phytoplankton are major concern in primary production estimation and prediction of probably algal blooms in aquatic ecosystems. The subject has important role in development and sustainable exploitation of marine culture. The goals of the project are study of chlorophyll-a concentration changes and its relations to variations of phytoplankton community structure parameters and abiotic factors (environmental and nutrients matters) in the costal waters of the Caspian Sea- Goharbaran region during 2013-2014. Monthly water samples were collected from different layers (surface, 5 and 10m) and depths (5, 10 and 15 m). The minimum mean (±SE) of abundance and biomass reported in spring (39± 9 million cells/m^3) and summer (94± 40 mg/m^3) respectively. The results showed maximum abundance (553± 58 million cells/m^3) and biomass (1209± 106 mg/m^3) in winter season. The minimum and maximum mean (±SE) values of chlorophyll-a recorded in spring (0.60± 0.05) and autumn (4.56± 0.23) mg/m^3, respectively. The changes trend of field chlorophyll-a concentration was confirmed by satelit data. Bacillariophyta showed the highest percent abundance in all seasons except in summer which it was for chlorophyta phylum. Pyrrophyta was the second dominant phylum in winter as well as spring; however its contribution in phytoplankton abundance of winter was low. The first dominant abundance species in spring, summer, fall and winter were Prorocentrum cordatum, Binuclearia lauterbornii, Thalassionema nitzschioides and Pseudonitzschia seriata respectively. Based on the results the species of Prorocentrum (scutellum+ proximum+obtusum) in spring and fall seasons, Cyclotella menenghiniana in summer and Pseudonitzschia seriata in winter showed the highest role in phytoplankton biomass forming. chlorophyll-a concentration showed significant Pearson correlations with biomass of total phytoplankton, bacillariophyta, pyrrophyta and chlorophyta phyla, dominant species, size cells of dominant species, water temperature, clearancy, nutrients matters. The study showed that chlorophyll-a cells content of winter dominant species was lower than fall dominant species. The Change of seasonal taxonomic phytoplankton pattern showed important role in relationship between chlorophyll-a cells content with biotic and abiotic factors. Meanwhile the values of temperature, nutrient matters, pH, pattern of dominant phytoplankton species showed significant roles on decoupling between chlorophyll-a and biomass changes pattern. The critical time of algal bloom recorded from September to January and March based on chlorophyll-a concentration. Spatial critical algal bloom was more obvious on surface water from October to December based on chlorophyll-a concentration. Pseudonitzschia seriata and Binuclearia lauterbornii species classified in medium bloom threshold (in winter and summer respectively) in all sampling depths. However Thalassionema nitzschioides (in fall) was in medium bloom threshold in 10 and 15m depths. As conclusion, in order to estimate logic primary production and predict algal blooms in the cage and pen culture sites it is necessary that all phytoplankton parameters such as chlorophyll-a concentration, biomass, abundance, shape, size, biological and ecological chracterstics of dominant species are considered. Because changes in the chlorophyll-biomass relationship could lead to obviouse errors interpretation of results and as well as unexpected field observations.
    Keywords: Biology ; Ecology ; Iran ; Persian Gulf ; Chlorophyll-a ; Algal bloom ; Abiotic Factors ; Marine Cultur ; Goharbaran
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25837 | 18721 | 2018-10-13 10:15:56 | 25837 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Shrimp is one of the most important aquatic animals,that have decreased their stocks in Hormozgan province, especially the eastern province . Evalution of food interaction in shrimp habitats (Estuaries of Tyab, Yek shabeh, Laft and Khouran) was studied with Ecopaht with Ecosim software (ver. 6.5) in Hormuzgan province. After entering the input components (Biomass,P/B and Q/B), model was balanced and outputs were extracted. Results of trophic levels, was close to previous research in these areas for all species in estuaries. Maximum of ecotrophic efficiency was belonged to phytoplankton and other preys that were observed in predator stomachs. Biological future of food web estimated to be threatened based on omnivore index of planktonivore groups. Maximum food competition between the four food models was estimated in detritivores communities in Laft estuary, that may be related to the low density of mangrove in this region. Khamir estuary ecosystem could be more stable, because each impacting group had its own separate ecological niche and special predation territory. key indices in each of four food models were related to phytoplankton communities, this key role has been demonstrated much better in Yek Shovy estuary than others. Future of commercial shrimp stocks will be expected to be in better condition in Khoran estuary compared to other three ones, due to selection of separate ecological niche by predators and High biomass of detritus in this estuary.
    Keywords: Biology ; Ecology ; Iran ; Persian Gulf ; Hormuzgan Province ; Ecopath ; Shrimp ; Habitats ; Estuary ; Assessment ; Dominant Species ; Food
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25081 | 18721 | 2018-08-22 07:08:58 | 25081 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: For the first time in Iran artificial propagation and larval rearing of one native species marine fish, Grouper (Epinephelus coioides) orange spont was done during - 1397. For estabishing marine fish farm site selection in Ghazaleh estuary in Mahshar was done. Estabishing netcages and wild spawner were caught and transfered there. Also marine hatchery farm containing spawning tank, trough, rearing larvae tank and live food unit were built. Because Grouper is hermaphrodite protogenos, it was difficult to provide enough male wild spawner naturally, so implantation technique was used for sex reversal. Successfully in supplying needed male spawner for propagation. In spawning season (April May) at 21-25 °C temperature two methods was used. 1. Induce spawning by injection Hormone HCG (1395) 2. Induce spawning without Hormone using by concrete spawning tank in natural condition (1397) all stages of propagation containing (ovation, fertilization, larval production and final food larvae and fingerling production, sex reversal) was successful. In sex reversal the best method was 17α metyltestostorn capsule Hormone implantation abdomen s fish whose suitable size was 5-6 kg .In artificial propagation, at 24-hour interval two Injection with HCG Hormone by 750 IU per kg fish was done. Spawner was started 24-hour after 2nd injection in large amount with bad quality and poor fertilization rate. In semi artificial spawning (without hormone) in spawning tank was gradually and from April at 2-25°C temperature to May 28-30oc temperature. Suitable water temperature for growth of larvae was (23-25°C) and survival rate was (% 29-1/2). The larvae were fed with small size rotifer (80-150 µ) on first week, with 10 rotifer /ml, and large size of rotifer (150-280 µ) on 20th day, artemia saline on (20-25)the day and after 30th day with minced meat. Finally fingerling reached to 9 gr in 80th day.
    Keywords: Aquaculture ; Biology ; Iran ; Khozestan ; Artificial prapagation ; Marine ; Grouper ; Artemia ; Sex reversal ; Epinephelus coioides ; Larval rearing ; Orange spont ; HCG ; Rotifer ; 17α - metyltestostorn ; Implantation
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25084 | 18721 | 2018-08-22 07:18:57 | 25084 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Through most of the last century, three endemic kilka species supported major commercial species in the Caspian Sea. It is clear that catches and abundance of all species have changed. Recent changes in the Caspian Sea ecosystem have occurred as a consequence of ecological change caused by the invasive ctenophore (Mnemiopsis leidyi). In this investigation which had been done in commercial catch regions during 2002-2004, in addition of biological characteristics survey such as species composition, length, weight, age, sex ratios and maturity stages; catch and catch per unit effort also were analyzed. During the years 2002-2004 the annual catches of kilka varied between 15000-25000 mt and CPUE varied between 1.077-1.474 mt (vessel×night). The frequency of anchovy kilka declined from 69.5% in 2002 to 26.9% in 2004. During the years 2002, 2003 and 2004 the frequency of common kilka were 30.4%, 48.9% and 71.9%, respectively. During this period the frequency of bigeye kilka was negligible. The average fork length of anchovy kilka increased from 100.4 mm in 2002 to 105.0 mm in 2004 and the average weight were 6.4 g and 8.4 g, respectively. The average fork length of common kilka increased from 87.0 mm in 2002 to 93.8 mm in 2004 and the average weight were 5.2 g and 7.4 g, respectively. Anchovy kilka spawn in spring and autumn but mass spawning anchovy occurred in autumn. Spawning of common kilka occurred in spring and early summer. In the age compositions of anchovy kilka, age 3 was the largest age group during 2002 and 2003 (55.3% and 52.1%, respectively). In 2004, age 4 predominated (42.5%). For common kilka, ages 3 and 4 predominated (representing 67.2, 62.0 and 69.1% of catches, respectively). According to CPUE and biological characteristics, after the appearance of ctenophore in the Caspian Sea, the abundance and biomass of bigeye and anchovy kilka collapsed.
    Keywords: Biology ; Fisheries ; Iran ; Caspian Sea ; kilka ; Species ; Ecosystem ; Mnemiopsis leidyi ; Survey ; Length ; Age ; Sex ratios ; Weight ; Maturity ; CPUE ; Anchovy Kilka ; Spawning ; Bigeye kilka
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25090 | 18721 | 2018-08-23 05:48:30 | 25090 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Mnemiopsis leidyi is one the species of comb-jelly. It belongs to the Ctenophora phylum and Lobata order. Mnemiopsis leidyi first appear in the Black Sea in 1980s and reached to the biomass levels up to 1.5-2 kg m^2 in the summer of 1989. This species is food rival of pelagic fish. It has been reported that the ctenophora caused the dramatic decrease of zooplankton and pelagic fish stock in the Black Sea. In this study, sampling on Mnemiopsis leidyi, zooplankton and phytoplankton started from July to December 2002 from a total of 2 stations located Anzali and Khazarabad by METU net in the Iranian Coasts of the Caspian Sea. Experimental studies on Mnemiopsis have indicated that Mnemiopsis begins to produce eggs in the Caspian Sea, when it reaches length about 15 mm. Although eggs were obtained even from specimen with 12 mm length and weight of 0.5 g. The most abundant size of reproducing Mnemiopsis was 20-30 mm in the Caspian Sea. Average fecundity of M.leidyi in the Caspian Sea was 1174±741 eggs/day with maximum 2824 eggs/day for specimens with length group of 30-39 mm and the weight about 2.0-2.7 g. Percentage of hatched eggs was not high in experiments with M.leidyi the range from 9 to 92 % after 24 hours. The maximum produce egg of M.leidyi measured with mean 845±733 eggs/day in Salinity of 11 ppt and the minimum egg recorded with mean 2 eggs/day in salinity of 4 ppt. The results showed that the biomass of M.leidyi had some fluctuation in different months and its maximum biomass was recorded in August with a figure of 384±156 g.m2 and the minimum biomass was observed in December with a figure of 87±46 g.m^2 . The gut contents of Mnemiopsis leidyi showed a wide variety of prey including 84 % of zooplanktons and 16% of Phytoplanktons.The most abundance of in taked foods was Acartia (belong to Copepoda) with 34 % and the least was podon polyphemoides (belongs to Cladocera) with 0.74 %. Also 3.0 % of fish eggs and 4.2 % of Lamllibrachia were found among stomach contents. The young specimen (5〈 mm) consumed more zooplanktons and phytoplanktons comparing to adult ones. Copepoda was dominant zooplankton in the Iranian coasts of the Caspian Sea. Fecundity of Mnemiopsis showed correlation to the length and wet weight in the Caspian Sea and reproduction of M.leidyi was done small size in comparing with other area in the Caspian Sea. Salinity, water temperature and food availability is the most important factor in producing eggs in M.leidyi. It seems, that Mnemiopsis leidyi feeding has been one of the most important factor in declining zooplankton populations and Clupeonella stocks.
    Keywords: Biology ; Ecology ; Iran ; Caspian Sea ; Anzali ; Khazarabad ; Mnemiopsis leidyi ; Diet ; Reproduction ; Species ; Ctenophora phylum ; Lobata ; Biomass ; Pelagic ; Ctenophora ; Zooplonkton ; Acartia ; Copepoda ; Podon polyphemoides ; Cladocera ; Salinity ; Temperature ; Clupeonella
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25095 | 18721 | 2018-08-22 07:17:48 | 25095 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The object of this survey is to enhance maturation of Astacus leptodactylus by temperature modification in order to have juveniles in the starting period of crayfish culture and to reach an acceptable market size at the end of one period culture as well. The research initiated from 27 November 2000 and lasted on 21 November 2001 at safidrood shilati research station. Crayfish brooders were catched by funnel trap from arass reservoir on late November 2000 and transferred to safidrood shilati research station , hold on concret pond as long as to observe eggs. Enhancing of the time of maturation conducted on one treatment and four replicate with raising temperature of the medium to 18-20°C. Four replicate used for control under natural temperature which ranged between 13.3 to 20.4°C with average 16.8°C. The dissolve oxygen concentration varied from 5.12 to 9.46 mg/l while Phof water were between 7.25 to 8.10 on mid April 2001. Egg development were observed on treatment which were one month earlier than control. Each crayfish produced 5 miniature with average individual weight of 50mg. The jovial of both treatment and control reared for seven days. Fed by dafni, chapped kilka and rice brain. To evaluate the relative growth between control and treatment, two soil pond with surface area of 100 m^2 were used for the culture. The duration of culture concerning to treatment was 210 days while for the control was 180 days.
    Keywords: Biology ; Iran ; Sefidrood ; Arass Reservoir ; Maturation ; Astacus leptodactylus ; Temperature ; Survey ; Crayfish ; Culture ; Brooders ; Eggs ; Dissolved oxygen ; Growth
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  • 137
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25101 | 18721 | 2018-08-23 05:22:05 | 25101 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study genetic variation of clupeonidea using restriction fragment length polymorphism (RFLP) of the mtDNA control region. Samples were collected from Amirabad port (50 individuals for each species) and Anzaly port (50 individuals for each species) regions in East and west of southern part of Caspian Sea. DNA was extracted from fin tissue by phenol - chlorophorm method. PCR was performed using D- Loop region, as result PCR product was about 1015 bp in all samples. The PCR products of samples ware digested by 19 restriction endonuclease enzymes. Ten out of nineteen restriction enzyme were polymorphic for D- Loop region. 12 different haplotypes were detected in C. engrauliformis and 9 haplotypes in C. grimmi and C. cultriventris. The mean value of haplotypes diversity among 71 Populations Was 0/786, 0/734, 0/426 and nucleotide diversity was 0/0092, 0/0099 and 0/0079, respectively. The nucleotide divergence among populations in C. engrauliformis, C. caltriventreis and C.grimmi was 0/01%, 0/01% and 0/003%, respectively. The results showed that the hoplotype differences in C. engrauliformis and C. cultriventris were significant (p〈0.01) and in C.grimmi was not significant (p〉0.01).
    Keywords: Biology ; Iran ; Caspian Sea ; Anzali ; Clupeonidae ; C. engrauliformis ; C. caltriventris ; C. grimmi ; mtDNA ; RFLP ; Genetic variation ; Clupeonella caltriventris ; Length ; Enzymes
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  • 138
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25099 | 18721 | 2018-08-22 07:24:00 | 25099 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Salinity effects on brooders spawning, growth and survival rate of yellow fin sea bream (Acanthopagrus latus) fingerlings was studied in Khouzestan Marine Fishes Research Station (Bandar-e Imam) in 2002- 2003. More than 200 brooders were caught in Mahshar creeks using hook, and 6 males and 3 females of brooders were introduced to each 4 tons tank. The experiments were carried out using 3 salinity treatments (30±1, 35±1 and 40±1 ppt) in 3 replications. Survival rate of brooders in 4 tons tanks during late January to 4^th April was estimated more than %90 in all salinities and the maximum rate was observed in 30 ppt treatment. Spawned brooders percentage in 40 ppt was more than the two other salinities, and spawning occurred in all three replications of this treatment. In all treatment, spawning started from early march (late lunar month) at 19~'C and continued at 23°C. Duration and replication of spawnings in 40 ppt were more than other salinities, and last about 20 days. Released eggs (2461046), amount of eggs production per day (66413), average number of eggs per brooder (312914), and average eggs per kg body weight (649460) were found in 40 ppt more than other salinities. Floating eggs ratio was more than %90 in 40 ppt and it was significantly different to the others. Fertilization and hatching rates in 40 ppt treatment (86.7, 67 respectively) were more than 30 and 35 ppt but there was no significant difference. All spawnings cases , released eggs hatched.
    Keywords: Biology ; Iran ; Khouzestan Province ; Bandar-e-Imam ; Mahshar creeks ; Salinity ; Spawning ; Yellow fin ; Bream ; Acanthopagrus latus ; Propagation ; Density ; Survival rate ; Fingerling ; Growth ; Acanthopagrus latus ; Yellow fin sea bream
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  • 139
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25103 | 18721 | 2018-08-23 06:08:50 | 25103 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study, circumstances of osmoregulation system of the Persian sturgeon released in the Gorgan River in order to stock restoration have been investigated. Ten days old larvae sampled during 45 days from hatchery to the estuary. Samples taken form blood serum, gills and kidney tissues were collected from 12 May to 26 June, 2003. In the present study, Sampling sites were Shahid Marjani sturgeon hatchery, earthen pond, river water (2 sub samples ) and estuary. The blood serum osmotic pressure ( mOsm/L) , K^ + , Na^+, Mg^++ ( mEqu/l) and Ca^++ (mg/dl) concentration were measured. In order to investigate the condition of osmoregulatory cells development, histological experiments of the specific tissues have been taken place accordingly.Regarding to figs. (1,2,3,4,5,6,7,8,9,10) , osmotic pressure of blood plasma was higher than the water ( the difference range is 14- 196 mOsmol/l). Statistical results (test of one way t student) show significant differences between the medium osmotic pressures (P=0.99). In cases of ions, the concentration of Na^+ and K^+ were higher than those of the water. There is also significant difference between these ions concentration (P = 0.01).In contrast, the content of Mg^ ++ and Ca^++ were lower than the water. Regarding to these last two ions, there is also significant difference (P= 0.99). Histological experiments confirm the results and show a relative development of the osmoregulatory cells in gills and kidney in accordance with fish ages.
    Keywords: Biology ; Iran ; Gorgan River ; Osmoregulation ; Persian sturgeon ; Plasma ione
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  • 140
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25097 | 18721 | 2018-08-22 07:19:46 | 25097 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Considering decrease in total catch of sturgeon & threat of extinction in their stocks, special measures might be adopted. Sperm cryopreservation is one of the suitable methods to prepare bank of frozen gamete for future use in artificial breeding in order to prohibit extinction of sturgeon stocks. This study carried out on 27 male sturgeon during 2001-2004. The investigated sturgeon include 12 male Persian sturgeon (Acipenser persicus), 7 male Acipenser stellatus, 5 male Acipenser nudiventris and 3 male Huso huso. Sperm Collected from spawners in Shahid Beheshti & Shahid Marjani sturgeon rearing & propagation Complex in Rasht & Gorgan. The Sperm which was collected from Shahid Marjani propagation complex placed in the sealed Vessels & transferred by coleman in near zero temperature to cryopreservation laboratory of international sturgeon research institute for further investigation. In this study, the sperm was diluted in ratio 1:1 in two culture media containing dimethyl sulfoxide & glycerol (BC) and the samples stored in 1 ml insulin syringe & 0.5 ml Piot. The diluted sperm froze in a special temperature by automated freezer model 5300 (France IMV). Three phases applied to freeze the samples which are as follow: 1- Begin to freeze from +50c to -100c (30c /min) 2- From 10 0c to 70 0c (20 0c /min) 3- From 70 0c to 130 0c (25 0c /min) after freezing, the samples placed in liquid nitrogen containers with 196 0c temperature. For thawing, the sperm samples took out of liquid nitrogen & placed in water 40 0c. Then motility percent & the sperm quality investigated under 400x microscope. According to the results the mean motility percentage of fresh sperm in Persian sturgeon, Acipenser Stellatus, Acipenser nudiventris and Huso huso was 84, 73.75, 67.5 and 76.66, respectively. The mean percentage of motility in frozen sperm which placed in media containing dimethyl sulfoxide was 32, 37.5, 40 and 20%, respectively. Also, the frozen samples that preserved in BC media (Biociphus) showed 5.2, 75.25, 4.11 and 2.66% motility, respectively. In blank group, the mean fertilization percent of eggs was 90, 72, 71.25 and 90%, respectively. In the treatment group applying frozen sperm in culture media containing dimetlylsulfoxide, the mean fertilization rate was 30, 6.5, 25.39 and 4.75%. Furthermore, no fertilization (0.0%) observed using frozen sperm stored in Biociphus culture media containing glycerol. There was no significant difference in fertilization percent comparing the two storage places (syringe & Piot). According to investigations the culture media containing dimethyl sulfoxide, is a suitable diluter for sturgeon sperm. So, the Cryopreservation technique can be used to preserve the sturgeon sperm for future fertilization & through this way we can prevent extinction of sturgeon stocks.
    Keywords: Biology ; Fisheries ; Iran ; Rasht ; Gorgan ; Cryopreservation ; Sperm ; Acipenser nudiventris ; Huso huso ; Acipenser stellatus ; Acipenser persicus ; Breeding ; Sturgeon ; Temperature ; Motility ; Fertilization ; Spermatozoa ; Freezing
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  • 141
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25098 | 18721 | 2018-08-23 06:04:47 | 25098 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In order to investigate some ecological condition of the waters of Bushehr province in the Persian gulf between 50~'-01 E to 52~'-47 E and 26~'-39 N to 29~'-03 N, 18 major and 16 minor stations have been studied from Nayband bay to Kharg Island, using R.V. Ferdows 1. There have been a preliminary survey in winter 2001, 4 seasonal surveys in 2001-2 and a complementary one in summer 2002. During this period the seasonal environmental conditions, physico-chemical parameters, zoo and phytoplankton concentrations in water layers, macrobenthoses and sediments were studied. Sea bed swelling in the Motaf region has resulted in division of the area in two parts and in a such away that the climatic and chemico-physical conditions in the southeast area are different from the northwest area. In the southeast area the termocline, halocline, picnocline and oxycline are generated in spring, increased in summer, moved down to the deeper layers in fall and disappeared in the winter. In the Northwest area the clines are only formed in spring and summer but disappear in fall and winter due to almost perfect water column mixing resulted from climatological conditions. During survey period the water temperature ranged from 18 34.1~'C, salinity 37.9 41.3 ppt, conductivity 51.3 70.4 ms/cm, sigma〈sub〉T〈/sub〉 23.1 30.2 g/cm〈super〉3〈/super〉, dissolved oxygen 0.2 8 ppm, and chlorophyll a 0.1 3.6 mg/m〈super〉3〈/super〉. It is observed that salinity, conductivity and sigma〈sub〉T〈/sub〉 have increased from surface to depeer layer, while oxygen and chlorophyll a had a pick in mean layers but temperature have decreased from surface to the depth. Temperature and conductivity decreased from southeast to the northwest and from costal to offshore. Salinity and sigma〈sub〉T〈/sub〉 increased from southeast to the northwest. Dissolved oxygen and chlorophyll-a did not show any trend. The concentration of nutrients including ortho silicate (Si-SiO〈sub〉4〈/sub〉〈super〉- 4〈/super〉), ortho phosphosphate (P PO〈sub〉4〈/sub〉〈super〉-4〈/super〉), nitrate (N NO〈sub〉3〈/sub〉〈super〉-〈/super〉 ) and nitrite (N NO〈sub〉2〈/sub〉〈super〉-〈/super〉 ) varied between 0.4 13.3, 0.05 1.6, 0.1 12.3 and 0.1 1.4 ~kmol/l, respectively. The overall nutrient concentrations increased from surface to depth. 61 genuses of 5 phytoplankton groups and 8 branches, 12 classes of zooplankton were identified. The identified phytoplanktons included 42 Diatom, 16 Dinophyceae, 3 Cyanophyceae, one Chrysophyceae and one Euglenaphyceae geneses. The highest intensity of phytoplanktons was recorded in summer, while in the case of zooplankton, was recorded in spring. Diatoms had the highest variety in all seasons and the maximum abundance in fall and winter. Cyanophyceaes were predominant group in the spring and summer. On the contrary to zooplanktons, the intensity of phytoplanktons decreased from coastal to the offshore waters. The highest intensity of all planktons (zoo and phyto) was in 10-20 m layer. The intensity of phyto plankton increased from southeast towards northwest. The zooplankton concentration didn t follow any particular trend. The identified macrobenthoses were 69 families including 34 polychaets, 22 gastropods, 10 of bivalvs, 1 crustacean, 1 scaphopoda, 1 foraminifera, 5 classes and 6 orders of crustacea, 1 order of echinodermata, 1 fish larva and 6 branches of other benthoses. Intensity and biomass of all groups were determined. The polychaets and malacostraca had the most abundance in contrast to the other groups. Macrobenthosis intensity decreased from coast to the offshore in southeast of Motaf region, while the middle stations of northwest of Motaf had a better intensity. The mean abundance and biomass of total area decreased from coast to the offshore, and the slope of decrease in biomass was more than abundance. Seasonal minimum and maximum abundance ranged from 1457 to 1903 per square meter in spring and winter, and the biomass ranged between 7.8-10.8 g/m〈super〉2〈/super〉 in summer and spring, respectively. In spite of high abundance of macrobenthose in the transect no: 13, the estimated biomass was lower than other transects. This might be due to prolonged exposition to more pollutant. The grain size of sediments was classified as gravel, sand, silt and clay. The organic matter of sediments ranged from 1.12 3.3% with annual average of 1.6%. The grain size of sediments in southeastern point was coarser than other areas. The middle stations of northwestern area had fine grain and more organic matter in contrast to the offshore and coastal stations.
    Keywords: Biology ; Ecology ; Iran ; Persian gulf ; Bushehr Province ; Hydrology ; Sturgeon ; Survey ; Phytoplanktons ; Macrobenthose ; Sediments
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  • 142
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25104 | 18721 | 2018-08-23 05:31:21 | 25104 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In order to evaluate the possible production of hybrids using two species of sturgeon; beluga (Huso huso) and Persian sturgeon (Acipenser persicus) a reciprocal crosses with three treatments and three replicates for each treatment was conducted. Reproduction normative including number of eggs per gram, fertilization rate, survival rate, and also 32 mophmetric and merestic parameters of parents, hybrids and control groups were compared. Genetic analysis of hybrid was conducted using two methods of cytogenetic (chromosome preparation) and molecular (microsatellite) techniques. Histological analysis was performed for sexual gonad development. The growth comparison between hybrids and control fish was conducted in fiberglass tanks for 18 months. Fish were fed using pellets and biometric measurements were carried out 17 times during the study period. Means, analysis of variance, standard deviation, Duncan s test and percentage of hetrosis were calculated using Quatro Pro and SPSS programs. Significant differences were detected between beluga controls and hybrids (male beluga x female A. persicus) and between A. persicus controls and hybrids (male beluga x female A. persicus) regarding number of eggs per gram (P 0.003). However no significant differences were detected between the control groups and hybrids regarding fertilization rate at the four celled and 35 celled stages, number of larvae produced, mortality rate up to the onset of exogenous feeding and the number of larvae surviving (P〈0.01). Growth rates differed in hybrid fish and fish in the control groups and highest weight increase at the end of the rearing period belonged to beluga control (975±10 g) followed by hybrids produced by crossing female beluga with male A. persicus (840±143 g), hybrids produced by crossing female A. persicus with male beluga (681.85±281 g) and lowest growth increases belonged to the A. persicus control group (535.15±131 g). Specific growth rate in the second and third six months of rearing in hybrids produced by crossing female beluga with male A. persicus was higher than those recorded in the beluga control group. Percentage of hetrosis was negative during the early rearing period (-18.93), however at the end of the rearing period offspring were superior to parents and percentage of hetrosis was 0.79. Comparison of 32 morphologic and merestic parameters showed significant differences between 23 parameters between beluga controls and hybrids and between 31 parameters between A. persicus controls and hybrids (P,0.05). The hybrids production was proved using the cytogenetic (chromosomal count) as well as microsatellite techniques. The number of chromosomes in hybrids was intermediate to the parents (2n=190 ± 9) and like all other sturgeon species, microchromosomes comprised more than 50% of the chromosomes. The chromosome number in hybrids was half the number of chromosomes in the parents (A. persicus 2n=258±4 and beluga 2n=118±3). With regard to the fact that the number of chromosomes in A. persicus is 4N and that in beluga is 2N the number of chromosomes in hybrids is 3N or triploid. DNA bands produced by PCR in parents and offspring showed genetic inheritance. Histological analysis of control fish and hybrids after 18 months of rearing showed that male and female cells were observed in hybrids that is a characteristic feature of impotent or sterile fish. However only one type of sexual cells were observed in fishes in the control groups (A. persicus and beluga). Results obtained from the present study show that the hybrids produced are triploid (3N) and histologically sterile. Also hybrids produced showed good growth. With regard to the scarcity of female beluga and the limitation in the production of beluga fingerlings, it is suggested that sturgeon hatcheries produce hybrids and thus meet the fingerling demands of sturgeon farms. Also considering that the hybrids produced are sterile they can be considered as a candidate for export for aquarium fish. With regard to the fact that the hybrids produced are a new species it is suggested that this species is named Belupars which is a taken from the names of the two parents Beluga and Persicus.
    Keywords: Biology ; Iran ; Caspian Sea ; Hybridization ; Acipenser persicus ; Huso huso ; Growth
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25269 | 18721 | 2018-09-07 07:39:31 | 25269 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In the changing environmental of the Caspian Sea specifically pelagic zone following the introduction of the comb jelly Mnemiopsis leidyi has been continued ,particularly kilka catch ,biological characteristic and feeding . In this investigation which had been done in commercial catch regions (where discharged theirs catch) in three ports Babolsar ,Amirabad (in Mazandaran) and Anzali (in Guilan), estimated catch and catch per unit effort, the age structure of catch ,length- weight relationship, von Bertalanffy growth parameters, condition factor, sex ratios ,maturity stags ,age at first capture and spawning and feeding .The result shown that catch of kilka in Iranian coastal in 2006 decreased from 22300 ton to 16700 ton in 2008.But CPUE increased from1.7 ton (Vessel ×Night) to 2.5 ton at that time due to dropped effort .The length frequency of kilka is close and the juvenile fish did not see in catch as increased the length mean (P〉0.000).The age frequency had the same situation as fish low than 2 years did not see for anchovy and bigeye ,so collapse juvenile stock cause poor recruitment in the species .The parameters of the von Bertalanffy growth curve were For common kilka L_∞= 136.5mm k= 0.249y t0= 1.890y Q= 3.883; For bigeye kilka L_∞= 131.7mm k= 0.346 t0= 1.123y Q= 3.688; And for anchovy kilka L_∞= 148mm k= 0.375 t0= 1.243y Q= 3.814 The exploitation rate of anchovy, bigeye and common kilka were 0.51, 0.58 and 0.6 respectively. The highest of GSI recorded in May and March for common and bigeye and in Jul, Nov for anchovy kilka .Main prey was Acartia tonsa by anchovy and common kilka and Cypris balanus by bigeye kilka. We concluded that the ecological problem causes by Mnemiopsis leidyi as well as overfishing has been collapsed kilka stocks, particularly anchvy.so sustainable fisheries management and conservation biodiversity in the Caspian Sea are real challenges now.
    Keywords: Biology ; Iran ; Caspian Sea ; Guilan Province ; Mazandaran Province ; Babolsar ; Anzali ; Kilka fishes ; Age ; Growth ; Feeding ; Overfishing ; Pelagic zone ; Mnemiopsis leidyi ; Bertalanffy ; Sex ratios ; Maturity ; Spawning ; CPUE ; Acartia tonsa ; Cypris balanus
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  • 144
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25303 | 18721 | 2018-09-07 08:28:00 | 25303 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The two groups of grass carp, have been propagated in North and South fish farms and then the larvae were transferring to Abziroshd and culture fish farm of Mazandaran. The fingerling at eight weight classes of 2 to 500 gram were investigated morphologically and some morphometric and meristic were compared. The South grass carp was significant difference (P〈0.05) with the North, in case of weight with the same length, number of vertebral and gill raker. The coefficient of variance (CV) of meristic characters of South Grass carp was in the range of 1.2 to 9.8 while in Mazandaran grass carp 2.8 to 8.6. This CV illustrate the low variance in both population. This research study showed that the two stocks can be regard as difference races from one ancestor.
    Keywords: Biology ; Iran ; Meristic ; Morphometric ; Grass Carp ; Population ; Survival rate ; Larvae ; Ctenopharyngodon idella
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  • 145
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25295 | 18721 | 2018-09-07 08:27:01 | 25295 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Location of family Satellite DNA (HindIII SatDNA) on chromosomes of Acipenser persicus and Acipenser gueldenstaedtii was analyzed using Fluorescent in Situ Hybridization (FISH) technique to determine the genetic differences between these two species. After obtaining suitable metaphase plates using leukocyte culture and HindIII SatDNA extraction from genome of the fish under study, extracted SatDNA from genome of A. gueldenstaedtii was used as a FISH probe. The probe was labeled with spectrum orange (Orange-dUTP) and hybridized with chromosomes of the fish under study. Analysis of SatDNA sequences isolated from A. persicus and A. gueldenstaedtii showed the presence of 163bp for A. persicus and 168bp for A. gueldenstaedtii. In the study of metaphase plates 66±4 signals were detected from the hybridization of the probe used with chromosomes of A. persicus and 68±3 signals were detected from the hybridization of the probe with chromosomes of A. gueldenstaedtii. Due to presence of numerous microchromosomes and heterogenous and large hybridization signals, it was impossible to identify the precise position of signals on chromosomes. The following results are worth mentioning: 1. developing leukocyte culture method for A. gueldenstaedtii and completing it for all of the Caspian Sea sturgeons. 2. Developing a suitable culture medium for the Caspian Sea sturgeons. 3. Isolating sturgeons HindIII Satellite DNA family from A. persicus and A. gueldenstaedtii, determining sequences and registering the sequences in the NCBI gene bank (FJ429174; FJ 94465). 4. Developing a protocol for FISH technique in A. persicus and A. gueldenstaedtii which has opened a new era in aquaculture genetic studies entitled Molecular cytogenetics in the country. Considering its potential applications, this technique can be applied to other aquatic species.
    Keywords: Biology ; Iran ; Acipenser persicus ; Acipenser gueldenstaedtii ; Fluorescent In Situ Hybridization ; HindIII SatDNA ; Molecular cytogenetics technique ; Chromosome ; Genetic ; Species ; Aquatic
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25308 | 18721 | 2018-09-08 16:30:57 | 25308 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In many species of finfish, females exhibit higher growth rates than males and achieve larger sizes. In addition, in some species, males mature before reaching marketable size. Therefore, there is great interest from the fish farmers to produce all-female stocks. In this project tried to reversing the sex of rainbow trout larvae by 17 estradiol and direct method, further more finding the optimum dose of this natural estrogen for endocrine sex reversion of Rainbow trout (O. mykiss). Four experimental treatments were designed with doses of 10, 20, 30 and 40 mg/kgf. Trout which treated with 40 mg/kgf yielded 96% female and greatest growth.
    Keywords: Biology ; Iran ; Rainbow trout ; Sex control ; Gonad ; 17-B Estradiol ; Species ; Finfish ; Females ; Males ; Growth ; Mature ; Larvae ; O.mykiss
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25117 | 18721 | 2018-08-26 16:47:59 | 25117 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Shrimp fisheries in the Persian Gulf have a long history. Since 1960 trawl fishery for Penaeid shrimp developed in the middle part of the northern coasts of the Persian Gulf (Bushehr waters). From 1998 to 2004 landing of shrimp have fluctuated between 480t and 2700t per year. The major species of penaeid shrimp (more than 80 percent) in the Persian Gulf and Bushehr waters is Penaeus semisulcatus. More than 2000 vessels and 33000 fishermen in Bushehr area were involving of shrimp catch (August and September) in study area in the recent decade. This species has an economical important role in the region. The present study was carried out in Bushehr coastal waters from 2003-2005. This study are described the bio-dynamic and life history of Penaeus semisulcatus in Bushehr area. Monthly samples of shrimp were collected by R/V LavarII from January 2003 to March 2004 for 15 months in 50 fixed stations. The carapace length frequency data were used to estimate the growth, mortality and exploitation rates by FiSAT program for males and females. The Von Bertalanffy growth parameters were estimated as K = 1.6 Year-1 and 2.2 Year-1 and L = 38mm and 50.4mm for male and female respectively. The results of mortality rates for males are Z = 6.4 per year F=4.3 per year and M = 2.11 per year. These results for female are Z = 8.2 per year, F=5.8 per year and M 2.41 per year. The exploitation rates for male and female are E=0.67 and E = 0.70 respectively. Relatively high values of total mortalities and exploitation rates were obtained which indicate that this species is overexploited. The maximum age was calculated 15 months for females and 18 months for males. Gonad maturity results were shown, the major spawning periods of green tiger prawn in the study area is in winter and early of spring and also there is a minor peak in autumn. Therefore two peaks of spawning were happened during this study. These periods are the main rainy seasons in the Bushehr area. The size at 50 percent mature (L50%) is 40.6mm and less than 5% of females were smaller than 24mm CL. Mature shrimps were aggregated in 20-30m depth of middle and southern part of study area. Imam Hassan, Genaveh and Helleh areas were the most important spawning grounds in Bushehr waters. The sex ration (male/female) of 0.84 was found during study period. This ratio was calculated in different months and the number of females were increased in spawning months. The number of 2571 shrimp were tagged and released by a green color polyethylene steamer tag during 15 months of survey and also before shrimping season (June and July) in 2005. The recaptures were done in two shrimp seasons in August and September 2004 and 2005 and 68 tagged shrimp recaptured that 4 recaptures were omitted due to inconsistencies information. Maximum recapture depths were 16-20m and 26-30m for males and females respectively. The majority of shrimp had a speeds of less than 2 Km/day, but maximum speed was recorded 3-4 Km/day in males and 2 Km/day in females. Only 10 percent of shrimp moved more than 50Km in study area and majority of shrimps, about 70 percent, were recaptured within 20Km of released area. The maximum recorded distance was 127Km after 95 days liberty in a female shrimp, but another female shrimp had only 20Km movement after 381 days and it was the maximum time between release and recaptured date. Tagged shrimp mostly moved to the northern part (Bahrekan area) of study area and recaptured shrimps pattern described stronger movement to the deeper waters. In the shallow waters of study area, sampling of juveniles of P. semisulcatus was carried out from July 2003 to March 2005. The survey was done after spawning months of this species and sampling were carried out in July, November and December 2003 and January, February, April, June, July 2004 and February and March 2005. Some stations in less than 10m depths were trawled by a small boat, equipped with a small beam trawl and the prawns (P. semisulcatus) less than 15mm carapace length were collected as juvenile. In this study 748 individuals shrimp were collected during study period. These juveniles were observed in July and November 2003 and April and June 2004. The carapace length of juveniles ranged from 3mm to 15mm and minimum of mean of carapace length were 4.5mm in November and maximum 9.6mm in July. The juveniles were observed around southern part (Mottaf) and middle part (Helaileh) of vegetated sites. A few juveniles were observed in non-vegetated areas. In the nursery grounds 47% of marine organisms were included other species that Sparidae, Platycephalidae, crabs, shells and other penaeid shrimp species such as Metapenaeus af inis and M. stebbingi were observed in nursery ground of green tiger prawn. Very few juveniles were caught during the colder months and seasonal abundance of juveniles was observed in the warmer months. Present study was shown that mature shrimp are dominant from December till March and juvenile stages were mostly observed in April to July. Spawning was occurred through the year, with two peaks minor in autumn and major in winter and spring. Fishing activity should be controlled in spawning and nursery ground of shrimp.
    Keywords: Biology ; Iran ; Bushehr waters ; Persian Gulf ; Green tiger prawn ; Migration ; Spawning ; Nursery grounds ; Platycephalidae ; Sparidae ; Penaeus semisulcatus
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25115 | 18721 | 2018-08-23 05:55:48 | 25115 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Amphipod crustaceans were collected from 251 spots in aquatic habitats of Fars province during July 2000 and May 2002. Different means such as aquatic nets or strainers, with strong handles and rings, were used to collect amphipods among macrophytes, leaves, roots, sands, and detritus. All collections were in daytime. To perform a systematic work, a "type‟ habitat was selected in each of the 9 station points in 7 water basins and 2 sub-basins in the province, and their populations became subjected to morphological studies. Precise drawings were made from different parts of the body (e.g. head capsule, eyes, mouth parts, antennae, pleosomes, epimeral plates, first coxal plate, key moving appendages like seventh pereopod, third uropod, and telson). These drawings were used to compare with existing identification keys. Extensive studies on each of these populations showed that they all belong to the family GAMMARIDAE, genus Gammarus and are members of the Gammarus pulex-group, an artificial group. It was impossible to identify these populations using existing keys; hence more subtle studies are needed. Regarding the so much diversity among amphipod populations in Fars, it seems that a specific identification key for this region must be produced. Some of important environmental factors were measured monthly during April 2001 and March 2002 in each of the selected stations, while others measured once in this year. These factors include some chemical factors including dissolved oxygen, pH, electrical conductivity, calcium and magnesium hardness, total hardness, chlorine, potassium and sulfate concentrations, and some physical factors including water temperature, depth, water current velocity and distance from the source, width, and discharge. Mean body lengths and wet weights of the 9 population were also measured in each of the habitats. All resulted data were analyzed using ANOVA, LSD, and Duncan statistical tests. Results showed great differences in average of temperature, electrical conductivity, dissolved oxygen, and depth. Some other factors like pH, and current velocity did not show any significant differences among these habitats. Body lengths and wet weights of the 9 population also showed clear and statistically significant differences amongst the populations. Putting together these results and morphological and morphometrical studies can help identifying the taxonomic situation of these populations. Collections showed that these animals are distributed in nearly-all, if not in all, fresh water habitats including springs, brooks, streams, and rivers in Fars province, with the exception of ponds, pools, or lakes _salty or fresh water_ and salty springs and rivers with more than 5 percent of salt (sodium chloride). Thus, these animals are distributed mainly in northern lotic habitats of the province, being extended southward to mid-south, and became sparse in eastern, western, and south fresh water warm springs and rivers. It seems that the southward dispersal of amphipods in this parts of the Province is limited primarily by salinity rather than temperature, or perhaps by a combination of the two. They also decrease in occurrence and number from west to east (away from the mountain regions). Also no populations of amphipods in southeast regions of the Kor water basin, including springs and brooks of Bamoo National Park were found, while their existence was reported from the here before. This could be due to the sever change in their habitats, probably as a result of agricultural fertilizers and toxins‟ pollution
    Keywords: Biology ; Ecology ; Iran ; Fars ; Amphipod ; Gammarus ; Geographical distribution ; Freshwater ; Ecology ; Taxonomy
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  • 149
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25114 | 18721 | 2018-08-23 06:10:56 | 25114 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Some specific and non-specific immune variables of grass carp against Aeromonas hydropila and in the present of organophosphate, diazinon was studied at 18-20?C. Also, the toxicity of this toxicant was assessed in this species. Isolation and characterization of the fish IgM showed that fish possesses IgM with molecular weight of 480-640 KD having 15-20% of total serum protein. The LC50 96 hours of the toxicant was 15.13 mg/L and the survival fish showed erythropenia, leucopenia and a decrease in levels of blood enzymes, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase and total protein, while level of glucose increased. When fish were exposed to sublethal concentrations of diazinon (1, 2 and 4 mg/L) for 12 hours large change were then observed in hematological and biochemical variables of blood feaures plus a suppression of chemiluminuscence (CL) response by blood leucocytes during 45 days post-expposure. When fish were intraperitoneally immunized with Aeromonas hydrophila whole cell antigens and exposed to 2 mg/L of diazinon, the immunological responses consisting of lysozyme content, CL response, antibody titre were suppressed during one moth post-exposure. Also, light and electron microscopic examinations of tissues of kidney, liver, gill, spleen and nostril of fish exposed to acute and sublethal concentration of the toxicant revealded remarkable changes including hyperemia, heamorrhage, necrosis, damaging of reticulum endoplasmic of epithelial cells and vacuolation of club cell of nostril, and degeneration of mithochondria in kidney cells, reduction in excretion of amorphous proteinous materials and vesicle numbers on the cell surface and also blocking of cell surface cannals of nostril cells.
    Keywords: Biology ; Health ; Pollution ; Iran ; Aeromonas hydrophila ; Diazinon ; Grass Carp ; Immune responses ; Organophosphate
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  • 150
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25127 | 18721 | 2018-08-26 13:33:35 | 25127 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The present study was firstly conducted to study the rate of sexual maturity in Nereis diversicolor under suitable conditions of temperature and photoperiod. The second objective was to determine the potential of artificial breeding in these worms for mass culture. Nereis diversicolor worms were collected from the Anzali lagoon in 4000 sampling operations during the year’s 2004 to 2006 using Ekman grab with a surface area of 400 cm2. The water salinity, temperature and total organic matter (TOM) of sediments in the sampling region was recorded. The worms were maintained in 0.5 tons (1 x 1 m^2) tanks containing clayey-muddy sediment to a height of 20 cm covered with 10 cm water (5 ‰) until they reached a weight of 200-300 mg. Sexual maturity in this species was attained at 4-6 ºC and spawning occurred at approximately 16 ºC. The first gametes were observed five weeks after the temperature increased from 6 to 16 ºC. Sexual maturity was studied at various salinities (0.5, 5, 12, and 15 ‰). Results indicate that these worms attained earlier sexual maturity at salinity of 15 ‰, compared to other salinities studied. No significant differences (P〉0.05) were observed between sexual maturity attained at 12 ‰ and 15 ‰. Stocking density (20, 50, 100, 150 worms) was studied in terms of sex and showed that number of females were higher than males and the ratio was 11:1 (female: male). No significant differences (P〉0.05) were observed between the various stocking densities and their replicates. The effect of light and photoperiod in synchronizing reproduction in male and female N. diversicolor was studied. It was evident that reproduction behavior in adult worms increased for a period of one week at the end of each month after they are exposed to a prolonged photoperiod (L:D=16:8) followed by a period of dim light (simulated using 1 W lamps). Feeding trials were carried out with Saccharomyces cerevisiae, formulated fish diets and humus. Results showed that this diet was effective in speeding up sexual maturity in worms and significant effect of treatment was observed (P〈0.05) in worms fed a diet of humus alone. Eggs and sperms were fertilized and worms developed from the young monotrochophore with jelly layer to the trochophore larvae.
    Keywords: Biology ; Iran ; Anzali Lagoon ; Reproduction ; Sexual Maturity ; Fertilization ; Nereis Diversicolor ; Culture ; Salinity ; Temperature ; Spawning ; Female ; Male ; Larvae ; Sacchromyces cerevisiae ; Density
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  • 151
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25139 | 18721 | 2018-08-26 16:57:20 | 25139 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Coregonus lavaretus is one the important species of coregonidae family. It has adipose fin and the tail fin has a deep notch. The fish is special for fresh and cold north waters and are mainly found in north hemisphere and north of Europe, Asia and America. It feeds aquatic invertebrate and zooplanktons. The mentioned species has tasteful meat and supplies the major part of fish consumption. The big fishes meat is tasteful, low fat and has a cucumber like odorous characteristics and is mostly consumed in dried and smoked forms. Since this fish is important in inland fisheries and is releasable and developing in water sources of lakes behind dams has been considered and respect to purity of water resources behind dams. In the lake of Karaj dam has been considered as the only habitat and considered to have to low biological information from October to late march 2001 for 6 months totally 82 samples were caught and studied and through this way lack of fishing in the region by determining 11 stations, the samples were collected and grown, relation between length and weight, age and length, propagation, nutrition and fertility parameters were evaluated. Most of caught fishes are 4 to 5 year olds and 3 and 4 year olds. The 4 year olds males were majority and 5 and 6 year olds females formed the dominant group. Correlation relation between age and total length in both sexes (male and female) was very significant at 1% level, of course in females the correlation was around 100%. The weight and total length in males and females that was caught both had high correlation at 1%level; of course this correlation in females had been very stronger than males. % level related to gonad weight and sexual evolution stages of females. Gonad had correlation at 5 the most absolute fecundity had belonged to 5 year old female with 19120 ovules and the lowest to 4 year old breeder. With 11496 ovules the most relative fecundity was 62.13% and belonged to 5 year old breeder and the lowest was 36.06% belonged to 4 year old breeder by increasing the quantity of ovules in breeders the sixth stage of sexual evolution the fecundity rate has been directly increased as well. % of Gonad weight was the most effective factor on absolute fecundity and according to (R2) more than 90 variations of breeders; absolute fecundity is claimable by their gonad weight. Among all traits, affecting gonad weight fecundity the breeders sexual evolution stage has been recognized the most effective factor on fish ovules diameter. Generally in oligotrophic aquatic resources they become mature before the age of 3 and in utroph aquatic resources and full of edible zooplanktons they become mature with 2 years of age. In respect to nutrition this fish eats a broad range of zooplanktons separated from surface to bed of water of dam lake and no kind of selection was seen there so that in different ecological condition it can differ appropriately to kind of foods available in different seasons so that in cold months (December to March) the food diet of this fish varies toward eating water bed living things such as tobifex.
    Keywords: Biology ; Iran ; Karaj Dam ; Coregonus lavaretus ; Species ; Coregonidae ; Aquatic ; Zooplanktons ; Fishes ; Fisheries ; Samples ; Gonad ; Evolution ; Fecundity ; Breeders ; Zooplanktons
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  • 152
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25142 | 18721 | 2018-08-26 17:01:32 | 25142 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was carried out to determine the appropriate size of Caspian trout (Salmo trutta caspius Kessler, 1877) juveniles for releasing to South Caspian Sea or possibility of cage culture in Caspian Sea water. 1611 specimens were exposed in 4 weight groups of 5, 10, 15 and 20 g, in 3 salinity trials: Caspian Water (11- 11.5), inshore water (7) and fresh water (control). Each trial was done in 3 replicates. The blood samples and tissue fixations carried out from juveniles of control group (in fresh water) and 3, 6, 12, 24, 72, 168, 240 hours after exposure of fish in different treatments. Plasma osmolal ity, Na^+ and Cl^- concentrations, were measured by osmometer, flame photometer, RA1000 respectively. Plasma cortisol level was determined by using RIA (radio immunochemical assay). Na^+, K^+-ATPase activity in homogenates of gills was estimated by phosphate released from ATP. Histological indicators including chloride cell diameter and nephron morphometric parameters were assessed using classic preparation and optic microscope with digital camera. Results of osmolality and ions measuring concurrently show that all weight groups can live in salinity of 7 and they maintain the osmolality and ion concentrations. In the Caspian water, weight groups excluding 5 g juveniles show same result. Mean plasma osmolality of 20, 15, 10 and 5 gr juveniles in control group (time of 0) were calculated 331.3±8.7, 307.7±6, 334.7±14.6 and 301±8.7 mosml/l. This parameter in the above weight groups after 240 hours exposure in the Caspian Sea water were measured 329±0.53, 321±9, 325.3±6.7 and 346.5±13.6 respectively. The observation of kidney glomeruli in histological sections shows that the diameter of glomeruli in 5, 10, 15 g weight groups in 7 and all groups in Caspian water decreased after 72 h adaptation period (p〈0.05). The cortisol level in 5g juveniles increased (p〈0.05) after 168 hour of the exposure (106±11.3 ng/ml) by comparison with control group (79.5±38 ng/ml). The changes of cortisol level in other groups were not significant. The number of gill chloride cells after 7 days of fish exposure in Caspian Sea and in water of 7 salinity was not changed significantly (p〉0.05) for 5g juveniles, whereas within weight groups of 10, 15 and 20 g in Caspian Sea water and groups of 15, 20 g in water of 7 salinity, the increase (p〈0.05) of chloride cells was observed. The amount of chloride cells on gill secondary lamellas in mentioned trials varied up 3 to 7 (between two lamellas on the histological sections of 5 thickness). The size of chloride cells in 5g juveniles in different salinities has not changed (p〉0.05), although in other groups, a significant increase of this parameter was detected during experiments. Na^+ ,K^+ -ATPase activity in juveniles of 5g weight group in 7 salinity and Caspian water was low (3.2 6.1 mol Pi /mg protein/ h). The enzyme activities in all weight groups were higher under the exposure in Caspian Sea water than that in water of 7 salinity. In group of 10 g juveniles at start time (control in freshwater) the activity of Na^+, K^+ -ATPase was significantly higher (p〈0.05) than that in 20g group. It is may be related to some metabolic changes and transforming to parr-smolt.
    Keywords: Biology ; Iran ; Salmo trutta caspius ; Osmoregulation ; Cortisol ; Kidney ; Chloride cell ; Age ; Releazing ; Evaluation
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  • 153
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25154 | 18721 | 2018-08-26 17:06:43 | 25154 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study spawning season, brood stocks live place and biological behaviour of Eleutheronema tetradactylum (rashgoo) was identified in the coastal waters of Bushehr province, Persian Gulf from July. 2005 to Sep. 2006. During this time fish samples were collected by the gillnet or set net. With the mesh size of 9 and 25 Cm. A total of 93 fishes were examined in the fishery Research Centre laboratory, Standard length, total weight, gonad and hepatic weight were measured and histological studies on gonads were conducted in this period. Gonadosomatic index was calculated in the different seasons we showed significant differences between research seasons (P〈0.05) the Maximum GSI was in winter and spring with (1-1/2 %) and minimum was in summer and autumn (/15-/22 %). In histological study of ovary had observed five stages in sections: IMMATURE, INITIAL MATURATION, ADVANCED MATURATION, MATURED and SPAWNED. And testicular tissues were showed three stages of the sperm development: Spermatogonia, Spermatocytes and Spermatides or sperm. This study had showed that Higher quantities of brood stock were caught in DAYER region of Bushehr province. The stomach contents observed that small fishes, shrimp and crab were feed by the caught fishes, it means that Eleutheronema tetradactylum (rashgoo).is a carnivorous fish. The results were obtained from this research showed that reproductive season in Four finger thread fine (Eleutheronema tetradactylum) (rashgoo) in the coastal waters of Bushehr was in the could seasons.
    Keywords: Biology ; Iran ; Persian Gulf ; Bushehr Province ; Behaviour ; Eleutheronema tetradactylum ; Spawning ; Ovary histology ; Broodstocks ; Samples ; Gillnet ; Gonadosomatic Index ; Immature ; Seasonal spawning
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  • 154
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25159 | 18721 | 2018-08-26 17:13:41 | 25159 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The alarming rate of population growth has increased the demand for food production in third-world countries leading to a yawning gap in demand and supply. This has led to an increase in the number of hungry and chronically malnourished people. This situation has created a demand for the formulation of innovative and alternative proteinaceous food sources. Single cell protein production is a major step in this direction. SCP is the protein extracted from cultivated microbial biomass. Algae, fungi and bacteria are the chief sources of microbial protein that can be utilized as SCP. Produced proteins from these microbes have various nutrition values. SCP is the manufacture of cell mass using microorganisms by culturing on available agriculture, industrial wastes and fisheries by products. Fish wastes due to high protein are the most important substrates for SCP production. In this study, SCP production was done from Silver carp and tuna fish wastes (head, tail and vise versa) and cooked water of canned tuna factories. The used microbes were six genus and species of yeasts include Candida utilis, Saccharomyces cereviceae, Rhodotorula, Khyveromyces marxians, Zygosaccharomyces rouxii and Bacillus subtilis and B.licheniformis. The examination was done in bench scale and CSTR bioreactor (Continuous Stirred Tank Reactor). The effects of various parameters such as pH, temperature, time, supplemented substrates, method of inoculation of microbes, rpm were evaluated. Changes of microbial growth and protein contents were tested by using Optical Density (OD) and Makrokjeldal methods respectively. In end of examination, produced protein were extracted and lyophilized. The results showed that protein percentage in bacterial protein was than yeast protein but wet percentage in bacterial protein was low. Production value produced from tuna fish wastes was higher than (30-45 g/l) to Silver carp wastes (25-29 g/l) and cooked water (10-15 g/l). By adding supplemented substrates, production value has been increased. Candida utilis, in comparison other yeasts, has high activation. B.licheniformis has also had more activation than Bacillus subtilis. The results of the effect some parameters on fermentation showed that yeasts and Bacillus in pH= 5.4 and 32oC and pH=6.9 and 35oC were better than growth pH=6 and 25oC and pH=6.5 and 30oC respectively. Time of fermentation in batch and bioreactor was 54 and 21 hours respectively. High rpm has been caused increasing of microbial growth in bioreactor. The conclusion showed that with optimizing of the growth condition such as some parameters (pH, temperature, substrates and so on) produced SCP with high efficiency. However, produced SCP should be exanimated with other specific tests such as amino acid and fatty acid profiles, minerals, nucleic acids and so on. After full examination, this SCP as probiotic could be used in fish and poultry feed.
    Keywords: Biology ; Iran ; Single cell protein ; Marine ; Culture ; Fish wastes ; Yeast ; Bacterial ; Population ; Growth ; Biomass ; Algae ; Fungi ; Nutrition ; Candida utilis ; Silver carp ; Bacillus subtilis ; B.licheniformis ; pH ; Protein
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  • 155
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25161 | 18721 | 2018-08-26 17:08:18 | 25161 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: For the first time in research program and species diversity the white legged shrimp (Litopenaeus vannamei) was introduced in Iran in 2005 by Iranian Fisheries Research Organization. In two recent years two projects was carried out successfully in Hele Research Station and according of these projects study of growth, survival rate and final harvesting of Litopenaeus vannamei impact of 25, 35 and 50 (per/m^2) stocking densities was done .Nine 0.4 ha earthen ponds were stocked from 26 June 2006 to 11 November 2006 and the rate of stocking was 100000,140000 and 200000 Pl in 25, 35 and 50 per /m^2 respectively. We also carried out daily test of pH, temperature and dissolve oxygen two times per day but transparency, depth and salinity one time per day. The mean weight in 120 days was 17/84±2/6, 16/03±1/9 and14/09±2/3 grams in 25, 35 and 50 per /m^2 respectively and highest weight was seen in 25 density. Results demonstrated that there was a significant relation (P〈0.05) between mean weight. The yield were 1745, 2055 and 2425 kg/pond although 4/3, 5/1 and 6 ton/ha in 25, 35 and 50 per /m^3 respectively. The maximum and minimum survival rate 97/96 and 88/46 was seen in 25 and 50 per/m^2. The minimum FCR 1.43 was seen in 25 per /m^2. For economical calculating just by using the cost of larva and feeding indicates that the highest benefit percentage was seen in 25 density. The average of salinity, pH, temperature and dissolve oxygen were 36.1-37 ppt, 8.1, 30.2-30.6 degrees centigrade and 5.9- 6.4 mg/l.
    Keywords: Biology ; Iran ; Bushehr Province ; Litopenaeus vannamei ; Density ; Mean Weight ; Species ; Diversity ; Shrimp ; Growth ; Survival rate ; pH
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  • 156
    Publication Date: 2021-07-16
    Description: Shrimp culture and rearing industry in particular white leg shrimp species (Litopenaeus vannamei) is one of the major activities in the world, including Iran. The outbreaks of diseases are the main factors limiting production. Due to the adverse effects of abusing antibiotics and chemical disinfectants, disease control and prevention requires novel methods that are affordable, effective and safe for the environment and humans. For this purpose during the five months, sampling was done from three main sites of shrimp culture in Bushehr province and 150 pieces cultured white leg shrimp, 135 water and sediment samples were collected from the ponds, input and output channels by standard methods. Physicochemical parameters of water and biometry of shrimps were done and recorded. Probiotic isolation was performed by culturing samples in Tryptic soy agar and TCBS after incubation in 30º C for 24-48 h. The antimicrobial effects of culture extract of isolates were evaluated against V. harveyi with the well diffusion method and the best isolates were selected. The molecular identification of selected bacteria was performed by 16S ribosomal DNA gene sequence analysis technique. The growth kinetics of selected bacteria and effect of environmental factors on antimicrobial compound production and stability of them in salinity (1.5-5.5%) and (0-50ppt), pH (5-9) and temperature (3040ºC) and (35-100ºC) were studied respectively. After the determination of antibiotic resistance profiles of them, the In vivo test on the L. vannamei post larvae was performed and effect of these bacteria on growth performance, prevention of vibriosis and water quality include (ammonia, nitrate nitrite, turbidity, total bacteria and Vibrionaceae load were examined. For extraction and identification of bioactive material with anti-bacterial properties produced by selected bacteria, dialysis, SDS-PAGE electrophoresis, ultraviolet spectroscopy and gas chromatography mass was performed. According to the results of this dissertation the most frequency of facultative aerobic and anaerobic heterotrophic bacteria was seen in shrimp digestion tract (3.04±0.75) ×105 CFU/g in September .Genus Vibrio spp. (37.88%) and Bacillus spp. (27.27%) had the most frequency respectively. Of the 198 bacterial isolates, two bacterial strains from the digestive tract and sediment which had the highest inhibition and a stability antagonistic effect on V. harveyi were selected. Based on 16S ribosomal DNA gene sequence analysis, they were identified as Bacillus subtilis subsp. inaquosorum strain IS02 (GenBank: JN856456.1) and Bacillus vallismotis IS03 (GenBank: JQ085958.1) and recorded in the National Center for Biotechnology Information (NCBI). According to results of identifying bioactive material produced by selected bacteria, in SDS-PAGE electrophoresis Bacillus subtilis subsp. inaquosorum strain IS02 did not have any band but Bacillus vallismotis IS03 had two band in 25 kDa and 34-45 kDa molecular weight which probably bacteriocin like substances and group III Bacillus bacteriocin respectively. UV spectra of selected bacteria were in the range (260-265nm) which related to peptide groups. In GC-MS the highest percent of chromatogram was belonged to pyrrolopyrazines in both of selected bacteria, which have antibacterial properties. Overall, according to results both of the selected bacteria were adapted to the ecological conditions of shrimp culture and could be used as appropriate probiotics in this industry. It is hoped that the results of this research is to produce a step towards the realization of national production of indigenous probiotic in Iran.
    Keywords: Aquaculture ; Biology ; Iran ; Bushehr province ; Isolation ; Molecular ; Probiotic Bacteria ; White leg Shrimps ; Litopanaeus vannamei ; Shrimp ; Culture ; Species ; Sediment ; L. vannamei ; Genus Vibrio spp. ; Bacillus spp. ; V. harveyi
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  • 157
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25184 | 18721 | 2018-09-03 17:16:24 | 25184 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: During last 65 years the catch of mullets had increasing trends with some fluctuations in the Iranian coastal water of the Caspian Sea .In this period about 138 thousand tons of mullets have been caught. Mullet’s account for 35% of total catch annually .In recent years species composition of mullets has chanched in the Iranian coastal water of the Caspian Sea and catch composition of golden grey mullet increased from 76% in 1995 to 98% in 2006. In this survey some biological characteristics of golden grey mullet have been studied in Iranian coastal water of the Caspian Sea .Fish samples have been gathered from commercial catch of beach seine cooperatives monthly in Iranian coastal water of the Caspian Sea over 2006 and 2007. In the laboratory fishes have been measured biometrically and biological parameters have been calculated .Also catch statistics of mullets during 2006-2007 have been obtained and discussed. Results showed that the catch of mullets in beach seine cooperatives during 2006 and 2007 was 4181 and 3685 tons respectively that golden grey mullet contribute 99% and 98% of the catch composition of mullets respectively. Length range of golden grey mullet caught by beach seine cooperatives was 19-50.2 cm with mean length of 32.7 ± 6.4 (± SD) and weight range was 67-1475 gr with mean weight of 411 ± 255 gr. The age structure of this species was comprised 2-10 years old fish with mean age of 4.42 years old. In this survey totally the sex ratio of male: female of golden grey mullet was 356: 434 that was significant variation from equal sex ratio. Pick of the spawning in Guilan province was in October and in Mazandaran and Golestan provinces was in November. In October the proportion of spawning females declined from western area (Guilan province) towards eastern parts (Golestan province).The highest proportion of spawning females was in December in Golestan province. The highest GSI index value was observed in September and October and it was decreased in November and December and it was consistent during January till April. The mean absolute fecundity was 700881±429987 eggs with minimum and maximum fecundity of 200112 and 2282862 eggs respectively. The Lm 50% for female golden grey mullet was calculated as 33.6 cm.
    Keywords: Biology ; Iran ; Caspian Sea ; Golestan province ; Guilan province ; Golden grey mullet ; Liza aurata ; Species ; Survey ; Samples ; Male ; Female ; Sex ratio ; Spawning ; GSI ; Fecundity ; Coastal waters
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  • 158
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25185 | 18721 | 2018-09-04 07:05:34 | 25185 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The dynamics of Artemia population of Urmia Lake was investigated to provide the technical information, which is necessary for sustainable management of Artemia in Urmia Lake. The study covers different aspects comprising of monthly estimation of Artemia biomass and cysts, annual evaporation of Lake Water, average precipitation in catchment areas and also water turbidity in Urmia Lake, during the 2004-2007. Results showed dramatic decline in the Artemia population compared to the previous years, Reduction of average annual precipitation and increased water usage in Urmia Lake catchment area during last decade, has reduced the annual quantities of flowing waters to the Lake. Although the estimated trophic state index (TSI) which indicated the mesotrophic condition during the studied period, therefore, exceeding salinity to higher than the saturated level had adverse effects on phytoplankton growth. Furthermore, nutrient transfer by the rivers to the lake caused to produce of halophilic bacteria and other obtrusive protozoas which has created very unsuitable condition for Artemia grazing. These events confirm the role of salinity as a major environmental factor that affects Artemia resources in their natural habitats. It can be proposed that a method to manage the lake ecosystem and its Artemia population through construction dividers, such as dikes between the north and south parts of the lake to reduce the salinity at some parts of Urmia Lake will be applicable.
    Keywords: Biology ; Ecology ; Iran ; Urmia Lake ; Artemia ; Population ; Biomass ; Cyst ; Turbidity ; TSI ; Phytoplankton ; Growth ; Nutrients ; Halophilic bacteria ; Protozoa ; Salinity ; Ecosystem
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  • 159
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25531 | 18721 | 2018-10-05 15:27:32 | 25531 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Blackmouth croackers (Atrobucca nibe) resources are the most important commercial stocks in the north-west of Oman Sea, exploited as lantern fisheries by catch with trawlers vessel. At least 50 specimens were sampled monthly from September 2012 to July 2014. Results showed blackmouth croacker have several small peak in gonado-somatic index, but the most frequency of adults occurred in May. The female: male ratio was calculated 1:0.8 and the LM_50 was estimated at 35 cm. The mean of absolute and relative fecundity were estimated 92988 and 223 respectively. LM_50 and LC_50 were calculated and the results showed this species mainly caught before length of maturity, and the mass populations have not sufficient opportunity to reproduction. The mean of egg diameter for black mouth croaker were calculated at 533±103. Linear model of Fecundity= 9597.6TL-233880 was calculated for length-fecundity relationship . Results of feeding showed lantern fishes (65%), shrimps (11%), Japanese threadfin bream (13%) and the other fishes (11%) were the main feeding regime of black mouth croaker. This species was carnivore, the food prefect mainly consisted of lantern fishes (57.7%) and shrimps (28.2%). The vacuity index (87.7 %) indicated this spices was low appetite fish. Total length range was 18 to 47.5 cm with mean length of 28.7 cm, and the class of 23-24 cm was the most frequent length in fishing ground. The length-weight relationships in A. nibe were calculated for female and male W= 0.0121L2.9385 and W= 0.0075L3.0737 respectively, both have isometric growth. Growth parameters of L_∞ and K were estimated 50 cm and 0.2 y^-1 respectively. Parameters of t0, Tmax and Ǿ were calculated -0.7 year, 14 year and 2.69 respectively. This species have two recruitment period, and the peak of recruitment was occurred in May with 23.14 percent. Total mortality , natural mortality and fishing mortality rates were calculated 0.53 y^-1, 0.46 y^-1 and 0.07 y^-1 respectively. Due to the calculated exploitation rate (0.13), it is appreciated the improvement of black-mouth croaker in the Oman Sea. Base on seasonal and monthly CPUE it was resulted the peak of fishing density were occurred in the winter. Results showed the main distribution of black-mouth croaker concentrated on the continental slope in 200 m depth, but these stocks move to deeper waters (more than 200m) in warm season and extend to shallow waters (less than 200m) in winter.
    Keywords: Biology ; Fisheries ; Iran ; Oman Sea ; Atrobucca nibe ; Reproduction ; Feeding ; Growth Parameters ; Mortality rates ; Exploitation rate ; Distribution ; Survey ; Black mouth Croaker
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  • 160
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25580 | 18721 | 2018-10-07 10:35:48 | 25580 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: By-products consist of more than half of the total weight of fish. Most part of these wastes made from connective tissue proteins that provide good potential to produce gelatin. Nowadays, consumers are more health conscious and have responded to the call for a diet that contains low calorie and low fat. This work involved the characterization of a low-fat spread made of fish gelatin as gelling agent, emulsifier and fat replacer. Six treatments with Fish gelatin (FG) to pectin ratio 1:1 and 2:1, with pectin concentration of 1, 2 and 3%, were produced and evaluated for quality changes and storage-life at refrigerated storage. The results showed that prepared emulsions were physically stable and no phase separation observed during 8 wks. The pH of treatments was 4.5 to 4.9. The moisture content of treatments varied from 68.9% for T1 (FG/pectin ratio 1:1) to 65.4% for T6 (FG/pectin ratio 6:3). The Max. lipid of samples was measured 28.4%. Max. and min. protein of samples were 3.8% for T6 and 1.6% for T2 (FG/pectin ratio 2:1), respectively. No significant differences (p≥0.05) were observed between ash content of all treatments and control (commercial margarin). The acidity of treatments increased during storage and were significantly different from production day (p≤0.05). Peroxide value (PV) of treatments increased significantly at refrigerated storage. No significant differences were observed between PV of treatments during different wks of sampling. The results of texture profile analysis showed that firmness, compressibility and adhesiveness properties were significantly increase with higher substitution of FG with pectin. Color, Aroma, Taste and texture properties of prepared samples were found to significantly lower than control, according to sensory evaluation. Significant increases were observed in mold/yeast and psychrophilic count of treatments during storage. The macroscopic growth of molds was observed on all samples in 8th wks. No coliform growth was observed in all treatments at any time. Results suggest that low-fat spread properties were significantly influenced by different ratios of fish gelatin and pectin incorporated. It seems that these results can provide new opportunities to develop market with introducing novel products to response consumer demands. Some quality defects observed in this study can be improved by using suitable machinery at industrial scale.
    Keywords: Biology ; Chemistry ; Iran ; Fish gelatin ; Low-fat spread ; Fish wastes ; Quality characterictics ; Texture profile ; Shelf-life ; Refrigerated temperature ; Cultured Carp ; Diet ; Lipid
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  • 161
    Publication Date: 2021-07-16
    Description: Caspian brown trout (Salmo trutta caspius) is an important from the point of economy. In this study genetic diversity of (Salmo trutta caspius) was investigated using microsatellite markers from two regions of the Iranian coastline of Southern Caspian Sea (Cheshmeh Kileh River in Mazandaran Province and Karganrud River in Gilan Province). The purpose of this research was the study of Salmo trutta caspius possible populations related to genetic diversity and population structure in the Caspian Sea and introducing the useful genetic markers. For this purpose the number 225 fishes from two Rivers were collected. DNA was extracted and amplified with 16 pairs of microsatellite primers using Polymerase Chain Reaction (PCR). The amplified products were loaded on polyacrylamide gels and bands were analyzed using Gene Alex and Pop Gene soft wares. Observed and expected heterozygosity averages were 0.46 ± 0.09 and 0.60 ± 0.10, respectively. According to the results from this study, Genetic polymorphism has been seen in this population.
    Keywords: Biology ; Iran ; Caspian Sea ; Mazandaran Province ; Gilan province ; Cheshmeh Kileh River ; Karganrud River ; Salmo trutta caspius ; Genetic diversity ; Microsatellite ; Molecular genetics ; Population ; Microsatellite ; Gene Bank ; Diversity ; DNA ; PCR
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  • 162
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25606 | 18721 | 2018-10-07 16:03:50 | 25606 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Abstract: Identification and classification of aquatic parasites are more important by the day. Aquaculture development are faced with parasites previously "not pathogenic in normal conditions, but under certain conditions bacame diseases and have caused heavy losses to the aquaculture farms. The results of these studies recognize the importance of parasites in fish production has clear economic value. Before any action for economic aquatic breeding the parasites that can infect the fish have been identified and methods of control will be drawn. The current project is a part (the fishes) of the project Parasitology Museum in the Faculty of Veterinary medicine of Tehran University. In the first phase of the project, it was planned the fish parasites from different regions of the country were collected and the Iranian Fish Parasites Data Base has also set up. Required experts and various specialties were organized for implementation an recognation of the collected parasitese. The province executive and coordination necessary and the methods of sampling were discussed after a day of training workshop was held at the Faculty of Veterinary medicine of Tehran University. Due to the lack of funds needed to coordinate the implementation, it was conducted that the parasite specimens collected from other projects. In the first phase of projects 261 parasites various have been sent. Collected samples received a temporary code and announced. In the laboratory the specimens categoried and the shape designed and finally the consultants send the final identification of the parasites. Posted parasites according to the latest international standards are maintained. Each parasite has a unique code that represents the name of the sender, verification of the final consultant, host and fishing region. Simultaneously, reported fish parasites from 1327 AD (1949 AD) were collected to create a database, they classified to be placed on the site inPersian and English. Fish parasites that have been reported are available in four types: final report of the research projects conducted at research centers and universities, student theses, abstracts published in scientific conferences and finally "published in national and international research journals. “Iranian fish parasitesd database” included: Founders and Pioneers Monument, Iranian fish parasite fauna, History, Search, Resources, Executive Committee, links, News. Researchers can search through the five “key words”: the name of the parasite, the parasite class branch, region or provincial fishing, infected of host organ. After achieving the desired list of parasites, descriptions, and specifications can be observe. The resource section lists some of the articles published and will be visible as a “pdf”.
    Keywords: Biology ; Iran ; Iranian parasitology Museum ; Fish ; Identification ; Classification ; Aquatic parasites ; Collection
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  • 163
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25228 | 18721 | 2018-09-06 05:04:27 | 25228 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Due to high maturation age in sturgeons and lack of morphologic differences between male and female even in brood stocks, sex determination is difficult in these species. In this research with using of AFLP approach and 100 primer combinations, male and female genomic DNA of 20 individuals in Persian sturgeon (Acipenser persicus) and beluga (Huso huso) were investigated. Ligation was carried out with using of MseI and EcoRI, and then adapters were ligated with using of T4 DNA Ligase. Fragments amplification was done through two steps PCR and electrophoresis on denature poly acrylamid and stained by silver staining. Data derived from banding patterns were scored as o (absence) and 1 (Presence). A set of 100 (Eco+3 and Mse+4) primer combinations in A. persicus and H. huso yielded approximately a total of 3771 and 3779 scorable bands, respectively of which 30% in A. persicus and 29.6% in H. huso were polymorphic. The fragments ranged from 50 to 600 bp without revealing any sex specific markers. So we used cDNA-AFLP approach in order to analysis of gene expression in 8 female and 8 male Persian sturgeon gonads. Results revealed two cDNA markers in female gonad (TDF1, TDF2) and they verified with RT-PCR in male and female gonads cDNA. But unfortunately they didn’t verify in genomic DNA. According to this research results and previous researches, it seems that sturgeons may have not sex chromosomes or the methods were used couldn’t determine them.
    Keywords: Biology ; Iran ; Persian sturgeon ; Beluga ; Sex marker ; AFLP ; cDNA-AFLP ; Investigation ; Identification ; Huso huso ; Acipenser persicus ; Molecular
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  • 164
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25799 | 18721 | 2018-10-13 08:36:21 | 25799 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this research was used sodium chloride in feed supplement to stimulate ion- osmotic regulation system of Rutilus Kutum juveniles (about one gram) in fresh water environment.The purpose of this experiment was to improve survival and growth ability of juveniles’ fish in brackish water during times of transition. The initial average weightes of Juveniles’ fish were 0.91±0.01 g (mean±SE). Experiments carried out in two phases. In step one, the Juveniles’ fish were kept for 15 days in fresh water and fed with commercial diet plus NaCl supplement (control without NaCl supplement, 5, 7 and 10 percent) in four treatments, each treatment inclusive three replication. In the second step, the fish transferred to brackish water (12.5 psu) and fed for 28 days with a commercial diet (without NaCl supplement). The results showed that no change compared to control treatment in renal tissue in step one. The difference between the kidneys were not in fish fed with NaCl supplement and without it. Nevertheless, a significant difference was observed between the size of the glomeruli and renal tubules between fresh and brackish water environments (between stage one and two), (P〈0.05). In step one, were observed in the gill accessory cells in treatments fed salt supplements. At the end of the second step, the number of gill chloride cells in fish fed with salt supplementation was more than control (P〈0.05). In addition, the highest and lowest weight gain was obtained in treatment two and four respectively (P〈0.05), and no significant difference was observed between control and treatment 3 (P〉0.05). The results showed that the fish fed dietary supplements of sodium chloride 10% versus the control treatment had less weight gain. Therefore, the was obtained the highest weight gain and growth performance (condition factor, specific growth rate) and the highest feed efficiency and protein efficiency ratio and the lowest feed conversion ratio in treatment 2 with the feeding of 5% NaCl in dietary supplements.
    Keywords: Aquaculture ; Biology ; Iran ; Rutilus Kutum ; Juvenile ; Salinity ; Turbidity ; Growth ; Gill ; Kidney
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  • 165
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25805 | 18721 | 2018-10-13 10:12:12 | 25805 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The limnological study of the Golabar dam showed that in the Glabar dam inspite of bing its early establishment due to high nutrient and organic matter is located in eutrophic stage. the pH as well as bicarbonate levels shows that buffering capacity of the lake is high and the value of inorganic and organic matter measured are not considered as limiting factor for warme and cold water aquaculture. In the plankton survey 44 species of phytoplankton and 25 species of zooplankton were identified. Cyclotella , Nitzschia, Synedra and Trachelomona from phytoplankton and Polyarthera , Keratella , Filinia , Pompholyx from zooplankton were the dominant spicies.the Bacillariophyta from phytoplankton with 76.5 percent and Rotatoria zooplankton to with 76.2 percent considered the highest abundant . The average frequency of phytoplankton and zooplankton were 5*106 and 723 individual per litter. The Shironomide and Tobificide were the only two bentic group were identified in reservoir wehre their mean frequced were 293.75 and 224.30 respectively. The average biomass of bentic organism were 1.44±0.97 gr/m2.In the survey 12 species of fishes were identified. The potential natural production have been estimated to be 2.8 to 15.5 kg for bentivorous fish and varied from 53 to 175 kg /hec for plankton consumer fishes .The low temperature in several months as well as ice covered of the lake surface in particular in the months of duty and Bahman are the limiting factor of either warm or cold water fish production.
    Keywords: Biology ; Ecology ; Iran ; Zanjan province ; Nutrients ; Phytoplankton ; Zooplankton ; Fish ; Benthic
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  • 166
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25813 | 18721 | 2018-10-13 10:17:34 | 25813 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In order to study feeding and reproductive biology of Southern meager (A.hololepidotus) in North West of Persian Gulf. 143 (74 males and 69 females) species were collected between October of 2008 to October of 2009 in coast of Khouzestan. Maximum and minimum length of fish were 143 cm and 81 cm respectively Maximum mean length (128.4±7.5) and Minimum mean length (104.86±12.47) were detected in October and May respectively. Maximum weight (27.5Kg) and Minimum weight (5.7Kg) and also mean weight to males (15222±1371) and Min mean weight of females (16942/02±2095) were detected. Absolute fecundity (4111847.66) and relative fecundity (268.33) were analyzed. GSI Analysis showed that these species (A.hololepidotus) spawn during June to September with highest spawning rate in September. Results showed that Southern meager is a batch spawner species relationship between length and weight of male Y=0.042L^2.70 (n=74,R2 =0.86) and female species Y=0.061L^2.61(n=69, R2=0.85) and also total species Y=0.059L^2.62 (n=143, R2=0.86) were measured. Due to not finding Immature species during sampling LM50 wasn’t analyzed. Analysis HSI and K index showed highest value in April and May and lowest value in August and September respectively. Prey occurrence index showed that fish is main food item and Crustacean specially Shrimp and Mollusca are secondary food item of this species. Analysis of vacuity index showed that females of Southern meager are more gluttonous than males and this species feed during spawning season. In general we can classify Southern meager as a moderate feeding rate species.
    Keywords: Biology ; Iran ; Persian Gulf ; Khouzestan Province ; Argyrosomus hololepidotus ; Feeding ; Reproductive ; Meager ; Species ; GSI ; Sampling
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  • 167
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    Publication Date: 2021-05-19
    Description: Kilka fish has been catch in Iranian coastal waters in Mazandaran and Giulan provinces. During in 1996 to 2000 Kilka fishing ships discharged theirs catch in three ports, Babolsar, Amirabad (in Mazandaran) and Anzali (in Giulan). In this investigation which had been done in commercial catch regions, in addition of biological characteristics survey such as species composition, length, weight, age, sexual and stages of sexual maturity, catch amount and catch per unit effort also were analyzed .The results shown that catch of kilka in Mazandaran and Gilan in 1999decreased from 28 and 57 thousand tons to 19.6 and 42.6 thousand tons, respectively. CPUE also decreased from 3900 kg in 1999 to 2500 kg in 2000.Between three species of kilka, anchovy dominated but the frequency of this fish decreased from 85-90% to 76% and the frequency of common kilka sharply increased. Common kilka had been caught in spring and summer but in 2000 this fish caught all months. Anchovy spawn in spring and autumn but mass spawning anchovy occurred in autumn. Spawning of common kilka occurred in spring and early summer. The length frequency of anchovy is close and the bigger fish did not see in catch. The average length of anchovy decreased from 96.3 mm in 1997 to 87.3 mm in 2000.Theage frequency had the same situation, as fish with 5+ and 6+ were not or had very low frequency. According to the reports mass group of Mnemiopsis leydei saw in 2000 in south of the Caspian. The animal damaged the pelagic stocks in Black Sea. Probably Mnemiopsis caused this mentioned characteristics in the Caspian Sea.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biology ; Catch ; Kilka ; CPUE ; Spawning ; Anchovy ; Common Kilka ; Mnemiopsis leidyi
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    Type: Report , Refereed
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  • 168
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    Publication Date: 2021-05-19
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Investigation ; Biology ; Tiger shrimp ; Shrimp ; Depth ; Penaeus monodon
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    Type: Report , Refereed
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  • 169
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    Publication Date: 2021-05-19
    Description: Among 13 fish species observed in Hamoon Lagoon, Chah-Nimeh of Hamoon basin and open water sources, Hamoon Mahi (Schizothorax zarudnyi) is one of the most valuable economic species in Sistan basin. This study performed during October 2001 to September 2002. Totally 697 Schizo/horax zarudnyi were examined, including 403 females and 294 males. Length and weight variation of samples were 24.5-62. I cm and 137-2204 g, respectively. The most weight and length frequency in samples were 500-600g and 35-40 cm. The GSI value has significant difference in males and females (p 〈%l). During June and February the minimum and maximum mean of GSI in female fish was 0 77% and 9.298%, respectively. The minimum and maximum mean of GSI value in males was 0.82% and 662%, which observed during July and February, respectively. During March and April, these items gradually reduced and has .no significant difference. The HSI increased before increasing of GSI. In females and males, HSI, increased during October to March and October to February, respectively. During significant increase in GSI (after September), the CF value was less than 1%. It was increased during March to June. There was significant difference in GI value between male and female fishes. The minimum of GI mean in males and females was 4.7% and 4.615% respectively, in February. In June, when exogenous feeding began, the maximum of GI mean observed which was 857% and 9.16% in males and females, respectively. The mean absolute and relative fecundity in fish weighing 460g to 1380g was 26256 and 34418 eggs, respectively. Fish weight has the most correlation coefficient with absolute fecundity(r = 0.64). The Lm50 in male and female fishes was 29-31 cm and 38-40 cm, respectively the correlation between lengths and weight in males and females were 0946 and 0.965, respectively. In probability level of 0.99, the sex ratio of male / female was not near to expected rate (l : l), Sexual maturation was divided into 6 separated phases. In March, female fishes show the most prevalence of phase IV of sexual maturation. In April, the prevalence of phase IV reduced and phase VI of sexual maturation increased, In female fishes which caught in Chah-Nimeh of sistan, phase V of sexual maturation was not observed. From May, phase [V of sexual maturation had not been observed and phase VI . had the most frequency (66%), after that phase II had the most frequency (about 33%). Ln male fishes, the most frequency of IV was observed in February (850/0), it was reduced to 70% in March and phase V of sexual maturation revealed (30%). In May, the ovaries of matured fishes examined and indicated one group of oocyte with less than 400g diameter. This trend continued till August (temperature was 22.31 Oc — 23.24Oc and length of day was 12.5-13.5 h ). In October, another group of oocyte having more than 500g diameter and new characteristics, appeared in ovary. The trend of oocyte growth continued that in March and April reached to its maximum rate (1600-2200g). Regarding many investigated indices, Hamoon Mahi is an Iteroparous species showing a synchronous ovary development and single spawner. It reveals the highest sexual maturity during March and April at 14-18 Oc. It is migratory Potamodromous species. That in March and April, during no flow water periods in the river and absence of migration of fishes to flowing waters: gonad in females are damage and resorbed
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Schizothorax zarudnyi ; Hamoon ; Reproduction ; Biology
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 113pp.
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  • 170
    Publication Date: 2021-05-19
    Description: Biology aspects of cobia (Rachycentron canadum) were investigated from October 2005 to December 2006 in Northern waters of Persian Gulf (Hormozgan province).The reproduction cycle, sexual maturity, sex ratio, Fecundity, Lm50, feeding, length frequency, growth parameters and mortality of cobia were studied from total of 509 specimens ranged from 19 to 159 fork length. Gonadosomatic index peaked during spring and summer with main peak in June. Spontaneous spawning occurs around the year with peak in June. The overall female to male ratio was significantly 1 : 1.49 (P〈0.05). Batch fecundity were estimated 1684954±118990 in 15 females. Relationship between total length and fecundity were calculated F=1.3717TL 2.9567 (r2= 0.82). Feeding studies indicated that the bony fishes were main food of cobia (76%) and followed by crustaceans (25%) and mollusks (11%). Rays were least food items (22%). Maximum and Minimum GaSI were observed in March (33%) and August (0.07%) respectively. Females reached 50% sexual maturity at 81.25 cm TL. The total length -weight relationship was W=0.0042L3.1162 ( r2=0.9852). Thetotal length-fork length relationship was TL= 1.1561FL-2.533 (r2= 0.9933). Growth parameters K, L ,and t0 were calculated by von bertalanfy growth equation : 0.11 (year1), 168.65 cm, 3.49 and -0.97 respectively. The instaneous rate of total mortality (z), natural mortality (M) and fishing mortality estimated by catch curve analysis were 0.30, 0.25, 0.14 (year1). Exploitation rate and Tmax were 0.36 and 28 yearrespectively.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Rachycentron canadum ; Cobia ; Sex ratio ; Fecundity ; Lm50 ; Feeding ; Mortality ; Growth ; Parameters ; Biology ; Maturity ; Spawning ; Fecundity
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 85pp.
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  • 171
    Publication Date: 2021-05-19
    Description: This study have been conducted entitle biological and statistical sturgeon in the southern Caspian Sea (Mazandaran waters)” from 2009 to 2013. Sampling was manthly. Overal, 1859 specimens of different species of sturgeon was caught including Acipenserpersicus (69%), A. stellatus (19%), Husohuso (6%), A. nudiventris (4%) and A. guldenstaedtii (2%). Totally, the meat and caviar of five species were found 53263.6 kg and 5633 kg respectively and caviar to meat ratio was 10.6%. The results show that ration of meat and caviar whole species reduced from 2009 to 2012 in which the meat and caviar of A. persicus was declined from 12146 to 4465 kg in meat and caviar from 1358 to 487 kg were extremely reduced respectively. The CPUE (catch per unit effort) of sturgeon has fluctuated from 2009 to 2012. Totally, the CPUE of A. persicus was decreased from 0.150 (2009) to 0.130 (2012) kg boat per day, A. stellatue from 0.009 (2009) to 0.021 (2012) kg boat/day, A. guldenstaedtii from 0.002 (2009) to 0.003 (2012) kg boat/day, A. nudiventris from 0.027 (2009) to 0.001 (2012) kg boat/day and H. huso from 0.123 (2009) to 0.018 (2012) kg boat /day. 1061 specimens of fish has been caviar including A. persicus with 69%, A. stellatus (18.1%), H. huso (5.5%), A. guldenstaedtii (2.7%). The frequency of caviar categorize was included 1 , 2, 3 and massive with 49.6%, 38%), 10.6% and 1.9% , respectively. The highest of meat and caviar belong to A. persicus with 31414 kg and 3515.7 kg, respectively were found and the lowest was 841kg and 105.9 kg pertain to A. guldenstaedtii. The results show that 65.9% of sturgeon was caught by legal catch (landing) and 34.1% by beach seine remained. 391 individuals was age determined that the minimum and maximum age was 9 and 41 year comprised to H.huso and A. stellatus, respectively. A. persicus and A. stellatus were majority caught compare to other species. A. persicus has the highest frequency in 14-15 year age groups with 50% and 57% in 2009 and 2011, respectively and 15-16 year age group with 41% and 56% in 2010 and 2012, respectively. The results show that mean of fork length, body weight, caviar weight of A. persicus was declined because the FL and weight average (±SD) obtained from 152.4±16.7cm and 25.8±9.2 kg in 2009 to 146.5± 18.7cm and 22.1±9.2kg in 2012, respectively. In contrast, the average (±SD) FL and weight of H. huso was increased from 218.8±41.1cm and 137.3±96.6kg in 2009 to 231.3±45.2cm in FL and 146.2±78.5kg body weight in 2012, respectively. Consequently, the results show that stocks of sturgeon in the Caspian Sea has a trend declined because of some species in IUCN categorize list was Endangered in 2006 but at this moment lie in Critically Endangered list of IUCN categorize were down which could be a serious alarm for the valuable sturgeon fish in the Caspian Sea. Therefore, recommendation that trade catch of sturgeon in the Caspian Sea must be forbidden and continue legal catch for spawner fish that needede to artificial propagation only.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biological ; Biology ; Sturgeons ; A. persicus ; A. stellatus ; A. guldenstaedtii ; A. nudiventris ; H. huso ; CPUE ; Survey ; Sampling ; Species ; Caviar
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 136pp.
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  • 172
    Publication Date: 2021-05-19
    Description: The Caspian goby, Neogobius caspius, is an endemic species of Gobiidae family in the Caspian Sea and it has ecological importance (as food for Caspian commercial fish such as sturgeons). The main aims of this study are, studying distribution, length, weight and age structure, sex ratio, diet, reproduction biology and morphological characters of this species in Guilan coast at waters of the Caspian sea and the sampling has been done monthly from Oct. 2005 to Sep. 2006 in 4 station from Astara to Chaboksar, with bottom trawl which had 12.5 meter length, 4.7 meter gape width and 2 mm mesh size in code end. The results showed N. caspius abundance is 9.82±11.93% of Gobiids and 7.92±10.10% of total fish numbers sampled in the study area (13824 specimens belong to 16 fish species) and there were significant differences among stations and seasons. CPUA of N.Caspius was estimated 52.5±105.9 ind/hec and 412.7±770.7 g/hec and there were significant differences among stations and seasons. Maximum body weight, total length and age were measured 65.10 g, 176 mm and 6 years old with an average 8.74±9.9 g, 84.83±28.4 mm and 1.83 years old, respectively and maximum age of males and females was 6 and 5 years old respectively and there were significant differences among stations and seasons and between sexes. It was estimated algometric growth model from length-weight regression, coefficient of K Von Bertalanffy growth model was estimated 0.42 and 0.68 in male and female, respectively. Sex ratio was 1.27 female to 1 male. Coefficient vacuity, relative gut length and intensity of 169 fullness were 8.72%, 0.76±0.17 and 267.7±263.3 for, respectively and Caspian goby fed on 30 different prey consist Zooplanktons (8 types), Benthic animals (15 types) and fishes (7 types) and young and yearlings have fed mainly mixed food (zooplankton and benthic animals) and adults on zoobenthose. Gammarids, Bivalvia, Cumaceae and Vormes constituted 44.7, 38.9, 24.8 and 19.7 % of prescence in full-gutted samples respectively, they were mainly preys (number and weight), hence this species is considered as euryphagus, carnivorous, benthphagus species. It was determined, Caspian goby spawns from March until last June, males and females take part in spawning in 2.8g and 65 mm and 1.3 g and 52 mm, respectively. Length at 50% maturity (LM50) was 80.2 and 77.5 mm in males and females, respectively. Maturity took place at 2 years old for both sexes, too. The absolute fecundity was estimated 109-1350 (451.6±216.3) eggs and relative fecundity 18-80 (36.8±9.2) eggs per one grams of body weight and diameter of ripe eggs was measured 1.31-2.60 (2.18±0.19) mm. Morphologically, there were 15 morphologically significant statistical differences between males and females and 15 differences among stations, sexual dimorphism was observed, too.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Ecological ; Commercial ; Biology ; Morphological ; Neogobius caspius ; Caspian goby ; Distribution ; Population structure ; Diet ; Reproduction ; Morphology ; Gobiidae ; CPUA ; Algometric ; Zooplankton ; Benthic
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 169pp.
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  • 173
    Publication Date: 2021-05-19
    Description: 0Genetic polymorphism by means of biochemical genetic markers using polyacrylamide gel electrophoresis system in four barbus fish species has been investigated. Species scientific name were Barbus sharpeyi ; Gunther, 1847 , Barbus grypus ; Heckel, 1843, Barbus xanthopterus ; Heckel, 1843 and Barbus esocinus ; Heckel, 1843.Sampling site and location were mainly in the rivers located in Khoozestan province such as Karoon and Karkheh rivers and the Dez dam. Different organs such as blood, muscle, kidney, eye and heart were sampled and analysed for the presence of tf, es, sod and pgm alleles. High polymorphism and presence of different alleles scored, but populations were not in the H-W equilibrium. Considring results, using current and avaiable genetic markers such as microsattelits is recommended for future works.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Chemistry ; Genetics ; Genetics Markers ; Distinguish ; Barbus ; Species ; Barbus sharpeyi ; Barbus xanthopterus ; Barbus esocinus
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 62pp.
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  • 174
    Publication Date: 2021-05-19
    Description: This survay has been done from Januray 2000 till May 2002 in Khouzestan costal waters. Four species of grouper were identified from which orange spotted grouper (Epinephelus coioides) was the dominant species. For studing environmental parameters and reproductive biology, age, growth parameters and mortality rate samples were collected by fishing ship. Samples were taken montly in 4 days by fishing traps and trawls. In addition, some samples were obtained from Khozestan fish landing centres. Environmental factors such as PH, 02, salinity, water temperature and depth of traping areas, were measured. To identify species, morphometric characteries of 452 individal fishes were measured. Stomach contents of 394 fish were has survaid, from which stomach of 226 fish, and 168 fish had empty stomachs. Percentage of empty stomachs (cv) in males was more than females. Food items found in 73 percent of stomach were crab (11%), shrimps (8.8%) , squids (3.9%), gastropods (17%) and bivalves (0.4%). Feeding intensity in year classes did not obay logic trends The importance relatively indicator (I.R.I) were 81, 9.9, 4, 1.5 and 0.3 percent for fish, crab, shrimp, squid, gastropod and bivalve respectively. For age determination, sagita otoliths of 450 fish were taken and countable sections were obtained from 425 specimens. Relative frequency distribution of opaque and transparent rings showed that each opaque growth ring generates once a year from November to September. It seemed that generation of opaque rings is affected by temperature and photoperiod changes. Correlation between length and age was calculated using Von Bertalanffy's least square method. Following equasion was obtaind: L(t) : 122.27 (1 e 0.146 (t+0.482)) Growth parameters were determined through by Ford Walford equasion and Response Surface and Shepherd subcommands in Elefan program and L00 and K amounts were have determined. Correlation between length and age of 635 fish was determined by gender . Length and age correlation was calculated by exponential model and between total length and standard length by straghit line model. Correlation between age and weight of sagita was calculated by total length and age. The most Correlation was between sagita weight and fish age (r=0.876). Total mortality rate (z) was estimated by Length Converthed Method , Jones and Vanzaling and Powel Wetherall. Total mortality rate was z=0.39. Natural mortality rate, using Pauly method was calculated M=0.32. Fishing mortality (F) was 0.08. Gonads of 425 fishes were surveid within 18 month, from which 363 were female, 46 were male and 16 were sex reversing individuals .Total length of females varied from 26 to 95.5 centimeters while males length varied from 56.5 to 107 centimeters. Sex reversing individuals had a length of 47.5 centimeters, when two years old and 62.5 centimeters at age of 3 years. From the mentioned 425 fish, 401 individuals were matured, containing 339 females and 62 males, 5.47 females against each male. Montly changes of Gonadosomatic Index (GSI) by total body weight and standard length and total body length showed that this index increases from march to May and maximum increase was in May . This experiment was adapted in spawning season. Potential, relative, and absoulate fecundity was estimated by counting eggs in three samples. Total amount of traped fish using special traps was 16182.18 kg from which Epinephelus coioides provided catching 15353.43 kg of it (91.27 %) and By catch was 141.18 kg (8.24 %). Total average CPUE for whole catch was 123.33 kg/day/vessel. Total amount of catch was estimated 232.04 tons, considering CPUE of total catch and total Khuzestan trap ships effort.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Fish ; Biology ; Distribution ; Growth parameters
    Repository Name: AquaDocs
    Type: Report , Not Known
    Format: 265pp.
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  • 175
    Publication Date: 2021-05-19
    Description: In order to study feeding and reproductive biology of Southern meager (A.hololepidotus) in North west of Persian Gulf. 143 (74 males and 69 females) species were collected between October of 2008 to October of 2009 in coast of Khouzestan. Maximum and minimum length of fish were 143 cm and 81 cm respectively Maximum mean length (128.4±7.5) and Minimum mean length (104.86±12.47) were detected in October and May respectively. Maximum weight (27.5Kg) and Minimum weight (5.7Kg) and also mean weight to males (15222±1371) and Min mean weight of females (16942/02±2095) were detected. Absolute fecundity (4111847.66) and relative fecundity (268.33) were analyzed. GSI Analysis showed that these species (A.hololepidotus) spawn during June to September with highest spawning rate in September. Results showed that Southern meager is a batch spawnerspecies relationship between length and weight of male Y=0.042L^2.70(n=74,R2 =0.86) and female species Y=0.061L^2.61(n=69, R2=0.85) and also total species Y=0.059L^2.62 (n=143, R2=0.86) were measured. Due to not finding Immature species during sampling LM50 wasn’t analyzed. Analysis HSI and K index showed highest value in April and May and lowest value in August and September respectively. Prey occurrence index showed that fish is main food item and Crustacean specially Shrimp and Mollusca are secondary food item of this species. Analysis of vacuity index showed that females of Southern meager are more gluttonous than males and this species feed during spawning season .In general we can classify Southern meager as a moderate feeding rate species.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biology ; Argyrosomus hololepidotus ; Feeding ; Reproductive ; Meager ; Species ; GSI ; Sampling
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 58pp.
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  • 176
    Publication Date: 2021-05-19
    Description: This Research has been done monthly in Sefidroud river and its tributaries from Agust 2003 to July 2004 . From the total number of 2027 specimens of caught fish , 800 and 629 were males and females respectively , and 598 specimens were undistinguished. Samples were caught by cast nets. Fork length range of all specimen was 33-255 mm (119.56 ±46) and their weight rang was 0.42-257.5 gr (34.4 ±39.06) gr and their age range was 0-5 (1.76 ±0.959) year. The avrege of relative frequency in C.c.gracilis was 65.1 % . The average of condition factor was 1.07±0.13 . Between Fork length and weight of C.c.gracilis . Was exponential relation and coefficient of correlation was estimated 99.11% . Growth cofficient (k) was 0.114 ; L∞ was estimated 373.5 mm. C.c.g consumed of Chrysophyta phylum more than other Phytoplankton phyla in Sefidroud river and from Bentic point of view, consumed of clay, Filamentous algae detritus, insects, seeds. Absolute fecundity range was 1823-9274 (4553.8 ±2169.1) eggs. Considering the GSI and frequency percent of maturity stages, spawning period of the fish begins from April and peak of spawning is in June and terminates in July.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biology ; Survey ; Capoeta capoeta gracilis ; Aquaculturize ; Species ; Specimens ; Males ; Females ; Fish ; Samples ; Weight ; C.c.gracilis ; Growth ; Chrysophyta phylum ; Phytoplankton phyla ; Filamentous algae detritus ; GSI ; Maturity ; Spawning
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 96pp.
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  • 177
    Publication Date: 2021-05-19
    Description: The project of the Study on fishing and resource management of fisheries within Southern Caspian Sea was conducted on the base of an agreement made between fisheries organization (Shilat) and the Iranian fishery research organization (IFRO) signed in 2010. In this library-based study the current situation of bony fish fisheries and biology of these species has been surveyed in Iranian waters of the Caspian Sea . In this survey internal and external environmental factors of bony fishes activities were considered. Using SWOT analysis method, current situation of fisheries was investigated..One of the most important strategies for conserving anadromous species such as Caspian kutum , common carp, roach , bream and …. is rehabilitation of the natural spawning ground in rivers, coastal lagoon specially Anzali lagoon .By conducting this strategy not only restocks of bony fishes is done ,but also reduced the cost of artificial restocking programs and releasing fingerlings .In short term , it is nessesary to enhance the stocks of the species that have low catch is done by huge releasing of the fingerlings and study the qualification of the program.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Environmental ; Bony fishes ; Distribution ; Stocks ; Biology ; Fishing ; Species ; Caspian kutum ; Common carp ; Roach ; Bream ; Spawning ; Fingerlings
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 117pp.
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  • 178
    Publication Date: 2021-05-19
    Description: Cheshmehkileh River and adjacent mountainous streams, play a strategic role as a historical axis for anthropogenic civilization, human welfare also habitat and migration pathway of commercial – biologic valuable fishes e.g. Caspian trout, Caspian kuttum, members of Cyprinidae family in south Caspian Sea drainage. Treats such as overfishing of Caspian trout and Red spotted trout stocks in mountainous headwaters, barriers construction and manipulations those are out of river carrying capacity developed by human activities, affected normal function of river as well. Sand mining big factories establishment next to the river, legal and illegal trade of river sediments, direct entry of Tonekabon landfill leakage into the river, development of Rainbow trout farms since 3 decades and huge effluents into the river containing dead fish and types of solids, escapement of cultured Rainbow trouts into the river, … are major minimum factors which needs basic information for integrating inclusively drainage management system. Cheshmehkileh River contains Headwaters of Dohezar (Daryasar & Nusha), Sehezar and Valamroud rivers during 13 monthly sampling phases between September 2009 and October 2010 based on macrozoobenthoses investigations by EPT, EPT/C EPA protocols, measurements of nominated physic-chemical and microbiologic parameters. Probability of Rainbow trouts escapement and invasion, existence, nutrition in Cheshmehkileh environment indeed investigated. Data analysis explained significant differences (P〈0.05) between groups of measured parameters in different sampling stations. Dendogram of clustered analysis based on consolidation of major biologic/ physic-chemical and microbiologic parameters, separated stations No. 1, 3, 2, 4 in one group and remained classified in different groups. Station 8 and 9 similarly separated which expressed general similarities according to Sehezar river environment which were differs in comparison with other stations. Station 11 separated according to its natural quality of water and environment. Similarities between station 10 to Sehezar river stations 8 and 9 expressed general influence of Sehezar River more than Dohezar River in Cheshmehkileh condition especially in station No. 10. High scores of EPT and EPT/C indices in upstream stations 1, 3 and 8 also low score of indices in stations 7, 13 and 6 expressed levels of environment quality between these groups of stations. Maximum average biomass of macroinvertebrates belongs to Trichoptera order in Cheshmehkileh River. Significant decrease of biomass in stations 11, 12 and 13 in comparison with other stations stated environment degradation in mentioned stations relevant to excessive sand mining as well. Pollution resistant groups of invertebrates significantly increased in downstreams against upstream stations. Also disappearing of Plecoptera order in station No. 7, 9, 10 and 13 stated low quality of environment in comparison with upstream stations. Confirmation of effects quality and quantity for point and non-point sources of imported pollutants require specific management considerations in order to present exploitations, pollutants control and emergencies for river monitoring in forthcoming years.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: River ; Pollution ; Aquaculture ; EPTC ; Assessment ; Macroinvertebrates ; Chemistry ; Microbiology
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 138pp.
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  • 179
    Publication Date: 2021-05-19
    Description: The Caspian Sea is the biggest lacustrine water source. Common carp is very important commercially in south coastal line of Iranian Caspian Sea waters, especially in east coastal line, because eighty percent annual catch of this species has been caught in this area in particular. Despite the common carp importance and value in Iran, but there is a little information about its biology in natural system. This study is comprehensive research on reproduction, growth and feeding. The specimens were caught by beach seine net in coastal line of Iranian waters. The fork length ranged between 5.6 and 2866.2 g. The b value of the length-weight relationship ranged from 2.843 to 2.924 for female and male, respectively. The age composition of the specimens was from 1 to 11 year. The gonadosomatic ratio (GSR) changed from 2 to 12. There were two peaks of GSR in April and December, so that the latter peak was much shorter than the first that. Fecundity variations were high and ranged 77448 to 430745 eggs. It observed linear significant correlation in fecundity- weight (r=0.98) and fecundity-length (r=0.88) relationships. Average growth in length was described with the Von Bertalanffy growth model: L (t) = 60.5(1-exp (0.19(t-0.65). The percent of empty stomach and prey dominant evaluated during different seasons by specific formula result showed that in Cyprinus carpio prey Molluscs was dominated and specific food items. Empty stomachs in winter and spring were higher and lower, respectively.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Cyprinus carpio ; Spawning ; Feeding ; Growth ; Common carp ; Biology ; Age ; Coastal line
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 51pp.
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  • 180
    Publication Date: 2021-05-19
    Description: Sampling of Astacus leptodactylusc were conducted in order to determination of biometrical and biological parameters suchas length , weight , sex ratio , fecundity and natural reproduction time . Two transect were selected at 49 34 and 49 36 geographical position on east Caspian Sea near to Anzali city . Metalic foulding trap with Silurus glanis as attractive diet were used to catch Astacus leptodactylusc At each line the traps were set on depth of 35,45 ,55 and 65 (5 trap at each depth) . Random sampling from each depth on tow lin for one year were conducted and the biometry performed on catched Astacus leptodactylusc where their sext uality and their ration were detemined for eacd month , season and whole year. absolute fecundity determined by cooking Astacus leptodactylusc , taking out the ovary weighing and counting them .Working fecundity assesed by separating eggs from their swiming leges while enomerate all egg . Complete randomized test of ANOVA for analysing the data were employed. The results showed average length and welght were calculated 122/07±1/74mm and 57/96±1/81gr respectively. Average absolute fecundity was 310/22 ±10/72 eggs , average working fecundity was 251/84±8/84 eggs , Average ovary weight was determined 4/31 ±619 gr and average number of eggs in one gr was 74/52±1/53 eggs .The sextual ratio in all year long was 1:1.32 . The reproduction season is about seven month from Febrary to July and the moulting of males occures two times each year. One of time is at may and the other is in September . The female molt thtina as the male start for second time.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biometrical ; Biological ; Biology ; Astacus leptodactylus eichwaldi ; Sex ratio ; Fecundity ; Silurus glanis ; ANOVA ; Female ; Male ; Coastal water
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 78pp.
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  • 181
    Publication Date: 2021-05-19
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Tenualosa ilisha ; Biology ; Fish ; Sex ratio ; Life history ; Ford Walford
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 244pp.
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  • 182
    Publication Date: 2021-05-19
    Description: The project of the Study on fishing and resource management of fisheries within Southern Caspian Sea was conducted on the base of an agreementmade between fisheries organization (Shilat) and the Iranian fishery research organization (IFRO) signed in 2010. The study has several phases. The final report of the fishery on fishing and resource management of fisheries within Southern Caspian Sea (Gilan, Mazandaran & Golestan provinces) is consisted of several sections such as: 1- The Survey status of biology and catch of Kilka fishes , Bony fishes and Estrogen fishes and also the status of aquatic resource conservation in the Southern part of Caspian Sea 2- Fishing analysis in the Southern part of Caspian Sea 3- Fisheries development Strategies, policies and plans in the Southern part of Caspian Sea In this survey and library-based study, internal and external environmental factors of shilat activities are considered. Using SWOT analysis method, current situation of fisheries is investigated. In this regard, all fisheries activities were divided into four groups, then, the strengths, weaknesses, opportunities and treats of internal and external environment will be analyzed. A considerable number of tables and matrixes were prepared and policies and strategies as seen of final report were carried out.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Resource management ; IFRO ; Shilat ; Fishing ; Kilka ; Fisheries ; Survey ; Biology ; Bony fishes ; Estrogen ; Environmental factors
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 84pp.
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  • 183
    Publication Date: 2021-05-19
    Description: Distribution of freshwater shrimp (Macrobrachium nipponense) in the freshwater ecosystems such as Alagol, Almagol, Ajigol lagoons, Gomishan lagoon and Gorganrud River. Sampling were done from may 2009 to my 2010 parameters such as, length frequency, growth rate, sampling, feeding, environmental factors and sedimentation were investigated. 3689 females and 8551 males were collected and biometrical parameters were recorded. Total length ranged between 3.2 to 98.7 mm. The parameters b of length-weight relationship in Alagol, Almagol, Ajigol were 3.49, 3.15, 3.45, 3.22 and 3.22, 3.28 for male and female, respectively. It shows in all lagoons for male and female growth is positive Allometric. Spawning period was from May to June. The length first at maturity (total and/or carapace) for three lagoons was 50.5 mm and 133 mm, respectively. The minimum and maximum of egg volume were 0.12 mm3 in Ajigol and 0.07 mm3 Alagol, respectively. The relationship between fecundity and length was found to be linear regression models for Alagol, Ajigol and nonlinear regression models for Almagol lagoon. Result showed that this species is omnivorous. The minimum and maximum temperature was 6.5ْ C in fall and 33ْ C in summer. The lowest and highest fluctuations of pH level in Ajigol and alagol lagoons were 8.2-9.2 and 7.2-9.8. Among three lagoons, the most EC was 5.9 Ms/c in Alagol lagoon. The Gorganrud River shows the maximum EC, 16.7 Ms/c.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Environmental ; Freshwater shrimp ; Macrobrachium nipponense ; Wetland ; Biology ; Ecosystems ; Sampling ; Growth ; Eggs ; Identification ; Distribution
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 111pp.
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  • 184
    Publication Date: 2021-05-19
    Description: This study have been conducted entitle biological and statistical sturgeon in the southern Caspian Sea (Mazandaran waters)” from 2009 to 2013. Sampling was manthly. Overal, 1859 specimens of different species of sturgeon was caught including Acipenserpersicus (69%), A. stellatus (19%), Husohuso (6%), A. nudiventris (4%) and A. guldenstaedtii (2%). Totally, the meat and caviar of five species were found 53263.6 kg and 5633 kg respectively and caviar to meat ratio was 10.6%. The results show that ration of meat and caviar whole species reduced from 2009 to 2012 in which the meat and caviar of A. persicus was declined from 12146 to 4465 kg in meat and caviar from 1358 to 487 kg were extremely reduced respectively. The CPUE (catch per unit effort) of sturgeon has fluctuated from 2009 to 2012. Totally, the CPUE of A. persicus was decreased from 0.150 (2009) to 0.130 (2012) kg boat per day, A. stellatue from 0.009 (2009) to 0.021 (2012) kg boat/day, A. guldenstaedtii from 0.002 (2009) to 0.003 (2012) kg boat/day, A. nudiventris from 0.027 (2009) to 0.001 (2012) kg boat/day and H. huso from 0.123 (2009) to 0.018 (2012) kg boat /day. 1061 specimens of fish has been caviar including A. persicus with 69%, A. stellatus (18.1%), H. huso (5.5%), A. guldenstaedtii (2.7%). The frequency of caviar categorize was included 1 , 2, 3 and massive with 49.6%, 38%), 10.6% and 1.9% , respectively. The highest of meat and caviar belong to A. persicus with 31414 kg and 3515.7 kg, respectively were found and the lowest was 841kg and 105.9 kg pertain to A. guldenstaedtii. The results show that 65.9% of sturgeon was caught by legal catch (landing) and 34.1% by beach seine remained. 391 individuals was age determined that the minimum and maximum age was 9 and 41 year comprised to H.huso and A. stellatus, respectively. A. persicus and A. stellatus were majority caught compare to other species. A. persicus has the highest frequency in 14-15 year age groups with 50% and 57% in 2009 and 2011, respectively and 15-16 year age group with 41% and 56% in 2010 and 2012, respectively. The results show that mean of fork length, body weight, caviar weight of A. persicus was declined because the FL and weight average (±SD) obtained from 152.4±16.7cm and 25.8±9.2 kg in 2009 to 146.5± 18.7cm and 22.1±9.2kg in 2012, respectively. In contrast, the average (±SD) FL and weight of H. huso was increased from 218.8±41.1cm and 137.3±96.6kg in 2009 to 231.3±45.2cm in FL and 146.2±78.5kg body weight in 2012, respectively. Consequently, the results show that stocks of sturgeon in the Caspian Sea has a trend declined because of some species in IUCN categorize list was Endangered in 2006 but at this moment lie in Critically Endangered list of IUCN categorize were down which could be a serious alarm for the valuable sturgeon fish in the Caspian Sea. Therefore, recommendation that trade catch of sturgeon in the Caspian Sea must be forbidden and continue legal catch for spawner fish that needede to artificial propagation only.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biology ; Sturgeons ; A. persicus ; A. stellatus ; A. guldenstaedtii ; A. nudiventris ; H. huso ; CPUE
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 98pp.
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