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  • 1
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    Induction of population triploid-interploid in rainbow trout (Oncorhynchus mikiss) using by indirect (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Induced polyploidy is a suitable tool for producing sterile fish which made commercial benefits in the aquaculture industry. This study carried out in order to produce triploid-interploid population via mating tetraploid female with diploid male rainbow trout. Heat shock was used for making tetraploid population and the best temperature and induction time were examined. Result showed that highest mortality from 1 day after fertilization to emerging were in groups 6 of embryos (40.1%) and the lowest were in groups 3 (33 %). Flowcytometry results showed that some fish were polyploidy. Comparative analyze of genome levels in tetraploid fish to control fish (diploid) and hen as standard indicator, confirmed tetraploid fishes in this study. In conclusion we can state that heat shock induction for 7 minutes at 65 hour –degree after fertilization in 28 ºC is optimum temperature for inducting tetraploid rainbow trout.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Sexual development ; Triploid interploid ; Tetraploidy ; Heat shock ; Flow cytometry ; Rainbow trout
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 43pp.
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  • 2
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    Production of monosex female population of Rainbow Trout by use of 17-B estradiol as direct method (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: In many species of finfish, females exhibit higher growth rates than males and achieve larger sizes. In addition, in some species, males mature before reaching marketable size. Therefore, there is great interest from the fish farmers to produce all-female stocks. In this project tried to reversing the sex of rainbow trout larvae by 17 estradiol and direct method, further more finding the optimum dose of this natural estrogen for endocrine sex reversion of Rainbow trout (O. mykiss). Four experimental treatments were designed with doses of 10, 20, 30 and 40 mg/kgf. Trout which treated with 40 mg/kgf yielded 96% female and greatest growth.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Rainbow trout ; Sex control ; Gonad ; 17-B Estradiol ; Species ; Finfish ; Females ; Males ; Growth ; Mature ; Larvae ; O.mykiss
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 32pp.
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  • 3
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    Estimation of Hormone Residuals(B- Estradiole) in Rainbow Trout (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Residual level of 17- Beta Stradiol and progesterone hormones in rainbow trout fish plasma were measured during different period using RIA method. Blood sampling from abdominal aorta were taken from 70 individual of female fishes (100±11 g) which had been exposed to hormone at 0.5, 1,2,4,8,12,24 and 168 h (7 groups) compared with control group which was not exposed to this hormone .Results showed that plasma hormones measurement in different fish groups after exposing had significant differences (P〈0.01) and the highest and lowest 17-Beta Stradiol hormone residue were observed in fishes that exposed 0.5h and 168h to hormone respectively (121±9 ng. ml^-1 and 3±0.9 ng. ml^-1 ) but there is no any differences between fishes exposed 168h to hormone and control group. Also the highest progesterone hormone level were measured in fishes 0.5 an 1h exposed and the lowest one was in fishes 168 h exposed. The range of progesterone hormone were between 0.3 to 1.1 ng .ml^-1 and significant increasing of this hormone levels were obtained in fishes exposed to hormone 4 to 24h (P〈0.01). As consequence these hormone can not residue in fishes for a long time and maximum after one week the levels back to the normal.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: 17- Beta Stradiol ; Rainbow trout ; Hormone residue ; Estimation ; B- Estradiole
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 35pp.
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  • 4
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    Investigation of chromosome and ploidy levels of Rainbow trout produced from imported eyed eggs. (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Rainbow trout is the main cultural species of coldwater fishes in iran. Often, aquaculturists intend to breeding in order to production of lines with higher growth rate potential and disease resistant. Nevertheless in the country, no trout breeding programs, has been performed yet and most of the farms focused on the cultivation of the first(unbred) race. While European countries progressed in trout breeding techniques and production lines with higher growth through genetic manipulation (chromosomal number and type changes of fish) and/or selection and their fish products derived from this technology, including eyed eggs and so on have sold to other regions of the world(eg: Iran). In this study, some biological parameters including survival, growth, feed conversion ratio (FCR.) and chromosomal number of two juvenile groups from imported( group 1) and native(group 2) eyed fish eggs were compared. For chromosomal investigation, blood smear test and flow cytometry were performed. sults showed a significant difference (P≤5%) in growth rate of native fishes and French group Native fish feed conversion ratio (0.9) was significantly difference (P≤5%) from that of French fishes (1.15). Chromosomal analysis showed no difference in chromosome number in treatments and two fish groups were 2n chromosome. Based on the results,the fishes of group 1 had faster grow potential and gain weight in less time than that of group 2 and this has been achieved to go through the process of selection and femenizatiom without any change in number of ploidy. Whereas the ability of native fishes in food efficiency( lower FCR.) was better. However, the reduction of rearing period is the benefit and preference of cultivation of imported or origionally foreign.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Imported eyed egg ; Native fish ; Ploidy level ; Growth ; Rainbow trout ; Chromosome ; Species ; Breeding ; FCR
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 30pp.
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  • 5
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    Broodstock Management, Reproduction, Cryopreservation in salmoind, Genetic Management and Selective Breeding in Responsible Aquaculture (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This study was conducted to codify the guideline and indexes of broodstock management in salmonid fish and cryopreservation, selective breeding and sex reversal in hatchery to attain responsible aquaculture development. Cryopreservation includes of sperm and egg in term of economic is important in aquaculture. In this report mentioned the type and name of material. All female and sex reversal fish are produced by steroid hormone which mentioned in this report. Selective breeding include individual selection and family selection which could be used for genetic improvement of fish.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Economic ; Broodstock ; Reproduction ; Cryopreservation ; Genetic ; Breeding ; Aquaculture ; Salmonid fish ; Sex reversal ; Hatchery ; Sperm ; Egg ; Female ; Steroid hormones
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 46pp.
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  • 6
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    The production of all-female in rainbow trout (Oncorhynchus mykiss) using indirect feminization (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The sex reversal technique in fish is widespread in many countries. The development of these techniques is desirable because rainbow trout males reach their gonad maturity earlier compared to the females. Rainbow trout alevins were treated with 17α-methyltestosterone incorporated in the diet (0.5. 1.5, 3, 6 and 10 mg/kg) for 60 days from the beginning of first feeding. Sex was determined at 180 and 680 dpf by sampling fish (n = 20) from each group and examining gross gonadal morphology under a dissecting microscope. Also sex reversal ratio and growth performance were determined in hormone-treated groups. These sex reversed functional males were reared for brood stock until they attained sexual maturity. At the end of experiment, normal rainbow trout eggs were fertilized with the sperms taken from sex reversed males for producing all-female populations. Examination of the results showed that 17α- methyltestosterone was effective in all treatment. The highest sex reversal ratio with 100% was observed in group treated with 0.5, 1.5 and 3 mg/kg 17α- methyltestosterone. The highest live weight ratios were observed in groups fed with 6, 0.5 17α-methyltestosterone and control group. Female progeny produced from the sex reversed males were 100 % in all males that sired offspring. All female trout stocks produced by this method have advantage in rainbow trout culture since the fish is not meant for direct human consumption and is used to generate brood stock, therefore, difference of growth parameters do not influence the success.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Sex differentiation ; Oncorhynchus mykiss ; Sex reversal ; Hormone treatment ; 17-αmethyltestosterone ; Female ; Population ; Steroids ; Gonad ; Maturity ; Rainbow trout ; Sexual
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 38pp.
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  • 7
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    Genetic improvement of rainbow trout in Iran (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Inthisstudy, Iranian and French male and femaleOncorhynchus mykiss broodstocks were invided into two groups 50 and 24 respectivelyin Research center of genetic and breeding of coldwater fishers, Yasouj,Iran and the genetic structure of them was investigated using 6 microsatellite markers. Along withbroodstockmaturation, fertilization 1:1(female:male) were randomly assigned and occurred in25 of12Iranian andFrenchtreatment respectively.Reproductiveparameterswere recordedfor the whole family.Average number of observed alleles in Iranian and French stocks was 6.68 and 6.83, respectively.Average number of effective alleles in Iranian and French stocks was 3.13 and 3.45 respectively.Fixation index Fstwas calculated based on allelic frequency between two stocks was 0.058 with significant difference between 2 stocks. Eyed percentage for french broodstock calaulated zero and deleted.Fertilization rate(100-0), the eyed percentage (98-0), The hatch rate(98- 0),the averagefecundity4114.708, the average eggs size 4.88 mm,Survivalinthe first three months 19-73% calculated for Iranian broodstocks.Considering thequality ofeggs and larvaeat different stagesandselectionbetween the differentfamilyandthe within family remained 10 treatments and are keptasfuturebroodstocks.Therelationship betweenfecundity-egg size, fecundity-weight,fecundity-length,egg sizeweightwas performedusingregression.The resultsshowed thatFecundity wasinfluenced more byweightandproductivelength.The research isbeginning toIDthebroodstockin our country.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Oncorhynchus mykiss ; Genetic ; Selection ; Rainbow trout
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 60pp.
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  • 8
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    Broodstock management, reproduction, cryopreservation in salmonid, genetic management and selective breeding in responsible aquaculture (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25651 | 18721 | 2018-10-14 02:48:20 | 25651 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This study was conducted to codify the guideline and indexes of broodstock management in salmonid fish and cryopreservation, selective breeding and sex reversal in hatchery to attain responsible aquaculture development. Cryopreservation includes of sperm and egg in term of economic is important in aquaculture. In this report mentioned the type and name of material. All female and sex reversal fish are produced by steroid hormone which mentioned in this report. Selective breeding include individual selection and family selection which could be used for genetic improvement of fish.
    Keywords: Aquaculture ; Biology ; Management ; Iran ; Broodstock ; Reproduction ; Cryopreservation ; Genetic ; Breeding ; Aquaculture ; Salmonid fish ; Sex reversal ; Hatchery ; Sperm ; Egg ; Female ; Steroid hormones
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 46
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  • 9
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    Investigation of chromosome and ploidy levels of rainbow trout produced from imported eyed eggs (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25825 | 18721 | 2018-10-13 10:43:05 | 25825 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Rainbow trout is the main cultural species of coldwater fishes in iran. Often, aquaculturists intend to breeding in order to production of lines with higher growth rate potential and disease resistant. Nevertheless in the country, no trout breeding programs, has been performed yet and most of the farms focused on the cultivation of the first (unbred) race. While European countries progressed in trout breeding techniques and production lines with higher growth through genetic manipulation (chromosomal number and type changes of fish) and/or selection and their fish products derived from this technology, including eyed eggs and so on have sold to other regions of the world (eg: Iran). In this study, some biological parameters including survival, growth, feed conversion ratio (FCR.) and chromosomal number of two juvenile groups from imported( group 1) and native(group 2) eyed fish eggs were compared. For chromosomal investigation, blood smear test and flow cytometry were performed. sults showed a significant difference (P≤5%) in growth rate of native fishes and French group Native fish feed conversion ratio (0.9) was significantly difference (P≤5%) from that of French fishes (1.15). Chromosomal analysis showed no difference in chromosome number in treatments and two fish groups were 2n chromosome. Based on the results, the fishes of group 1 had faster grow potential and gain weight in less time than that of group 2 and this has been achieved to go through the process of selection and feminization without any change in number of ploidy. Whereas the ability of native fishes in food efficiency ( lower FCR.) was better. However, the reduction of rearing period is the benefit and preference of cultivation of imported or origionally foreign.
    Keywords: Aquaculture ; Biology ; Iran ; Imported eyed egg ; Native fish ; Ploidy level ; Growth ; Rainbow trout ; Chromosome ; Species ; Breeding ; FCR
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 30
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  • 10
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    Induction of population triploid-interploid in rainbow trout (Oncorhynchus mikiss) using by indirect (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25827 | 18721 | 2018-10-13 10:47:16 | 25827 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Induced polyploidy is a suitable tool for producing sterile fish which made commercial benefits in the aquaculture industry. This study carried out in order to produce triploid-interploid population via mating tetraploid female with diploid male rainbow trout. Heat shock was used for making tetraploid population and the best temperature and induction time were examined. Result showed that highest mortality from 1 day after fertilization to emerging were in groups 6 of embryos (40.1%) and the lowest were in groups 3 (33 %). Flowcytometry results showed that some fish were polyploidy. Comparative analyze of genome levels in tetraploid fish to control fish (diploid) and hen as standard indicator, confirmed tetraploid fishes in this study. In conclusion we can state that heat shock induction for 7 minutes at 65 hour –degree after fertilization in 28ºC is optimum temperature for inducting tetraploid rainbow trout.
    Keywords: Aquaculture ; Biology ; Iran ; Sexual development ; Triploid interploid ; Tetraploidy ; Heat shock ; Flow cytometry ; Rainbow trout
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 43
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