Publication Date:
2021-05-19
Description:
This investigation was carried out in order to study of Morphological and genetical difference of various
cysts, identification of different ganetical populations and possibility of strains among populations. After
assessing the density of cysts at certain interval from different layers of Urmia Lake, isolation of cysts from
superficial and depth surfaces with salinity of 170ppt were done. Then, cysts from three stations isolated with
sensitive devices. For studying of quality and slightly quantity of cysts, hatching percentage and biometry of
cysts were carried out. DNA extraction with using phenol chloroform method from 210 samples of Artemia hatched were done. Population differences of floating cysts of Artemia urmiana were done by analyzing the results of RFLP of
Ribosomal RNA (rRNA) gene, which is on mtDNA, was amplified by PCR techniques. In order to enzymatic
digestion of PCR products by eleven enzymes Alu I, EcoR I, ECO47 I, Hae III, Hind III, Hinf I, Mbo I, Msp I, Rsa I, Taq I, Tru I were used. Two haplotype (AAAA, ABAA) showed similarity between cysts in those regions. To investigate the results Reap software and X2 test were used. The average of nucleotide diversity and
haplotype diversity with in population were 0.006547±0.000003 and 0.5419±0.00292 and nucleotide divergence
is estimated to be %0.0228. Based on the results, distribution of haplotypes in different geographical regions of Urmia Lake showed a
notable difference and indicating genetical diversity in the Artemia stocks. Finally, investigation in the produced genotypes showed that PCR-RFLP technique with using Hinf I as a
molecular marker can be use to separate the Artemia orumiana cysts in Urmia lake.
Description:
Iranian Fisheries Science Research Institute
Description:
Published
Keywords:
Morphological
;
Genetical
;
Variation
;
Artemia
;
Artemia urmiana
;
Identification
Repository Name:
AquaDocs
Type:
Report
,
Refereed
Format:
45pp.
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