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  • 2
    Publication Date: 2021-05-19
    Description: Population genetic structure of Vimba vimba persa was investigated using microsatellite markers from 4 regions along the Iranian coastline of Southern the Caspian Sea (Anzali lagoon & havigh River in Gilan province, BabolRoud River in Maz&eran province & GorganRoud River in Golestan province). Genomic DNA from 121 specimens was extracted from fin tissue by phenol-Chlorophorm method & PCR reaction was accomplished with 17 microsatellite primers, out of 17 microsatellite primers 14 loci were amplified, in which 10 of them were amplified with reasonable polymorphism & 4 were monomorphism. Totally 302 alleles were identified on average 7.5 Observed & expected heterozygosity averages were 0.80 & 0.77 respectively. Most cases significantly deviated from Hardy-Weinberg equilibrium (p 0.01). The estimation of Fst (p 0.01) revealed significant population structuring & estimate four population of Vimba vimba persa is identified in the Caspian Sea. These studies were to apply & develop population genetic approaches to assist conservation, sustainable harvest & restocking of these populations.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Vimba vimba persa ; Population ; Genetic ; Microsatellite ; DNA ; Genomic ; Specimens ; PCR ; Equilibrium ; Restocking
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 61pp.
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  • 3
    Publication Date: 2021-05-19
    Description: Population genetic structure of Vimba vimba persa was investigated using microsatellite markers from 40 regions along the Iranian coastline of the Southern Caspian Sea (Anzali lagoon and Havigh River in Gilan province, BabolRoud River in Mazandaran province and GorganRoud River in Golestan province). Genomic DNA from 121 specimens was extracted from fin tissue by the Phenol-Chlorophorm method and PCR reaction was accomplished with 17 microsatellite primers, out of 17 microsatellite primers 13 loci were amplified, in which 10 of them were amplified with reasonable polymorphism and 3 were monomorphism. A total of 302 alleles were identified on average 7.55. Observed and expected heterozygosity averages were 0.80 and 0.77 respectively. Most cases significantly deviated from Hardy-Weinberg equilibrium (p≤0.01). The estimation of Fst (p≤0.01) revealed significant population structuring and an estimation of the four population of Vimba vimba persa was identified in the Caspian Sea in which restocking of these species should be considered.
    Description: Published
    Keywords: Vimba vimba persa ; Population genetic ; Microsatellite ; Genetic ; Structure ; Population ; DNA
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.347-357
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  • 4
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    In:  http://aquaticcommons.org/id/eprint/22518 | 18721 | 2018-04-16 21:57:58 | 22518 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-06
    Description: Population genetic structure of Vimba vimba persa was investigated using microsatellite markers from 40 regions along the Iranian coastline of the Southern Caspian Sea (Anzali lagoon and Havigh River in Gilan province, BabolRoud River in Mazandaran province and GorganRoud River in Golestan province). Genomic DNA from 121 specimens was extracted from fin tissue by the Phenol-Chlorophorm method and PCR reaction was accomplished with 17 microsatellite primers, out of 17 microsatellite primers 13 loci were amplified, in which 10 of them were amplified with reasonable polymorphism and 3 were monomorphism. A total of 302 alleles were identified on average 7.55. Observed and expected heterozygosity averages were 0.80 and 0.77 respectively. Most cases significantly deviated from Hardy-Weinberg equilibrium (p≤0.01). The estimation of Fst (p≤0.01) revealed significant population structuring and an estimation of the four population of Vimba vimba persa was identified in the Caspian Sea in which restocking of these species should be considered.
    Description: Pages 356 and 357 are misnumbered. Article includes abstract in Farsi on last page.
    Keywords: Biology ; Fisheries ; Vimba vimba persa ; Population genetic ; Microsatellite ; Caspian Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 347-357
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  • 5
    Publication Date: 2021-07-15
    Description: The Mendelian Inheritance of produced hybrids from Chalcalburnus chalcoides and Vimba vimba persa was investigated using Microsatellite Markers. Genomic DNA from the produced larva and also small piece of dorsal and pectoral fin of their parents were extracted by Phenol-Chlorophorm method and PCR reaction was accomplished. 10 loci were amplified, in which 7 of them were amplified with reasonable polymorphism and 3 were monomorphism. In order to study the segregation of the produced F1 hybrids from female Chalcalburnus chalcoides and male Vimba vimba persa, seven loci (LCO3, LCO1, Lid11, Rru2, Z1-2, Z7-8, and Z9-10) were used. According to the results it showed that parents and F1 larva‘s allelic distribution were almost segregated based on Mendelian inheritance in 7 loci. The present study showed that Microsatellite markers are useful marker in study of inheritance.
    Keywords: Biology ; Mendelian inheritance ; Micro satellite markers ; Chalcalburnus chalcoides ; Vimba vimba persa ; Microsatellite ; Females ; Males ; DNA ; Genomic ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 50-58
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  • 6
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25259 | 18721 | 2018-09-07 07:41:54 | 25259 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Population genetic structure of Vimba vimba persa was investigated using microsatellite markers from 4 regions along the Iranian coastline of Southern the Caspian Sea (Anzali lagoon & havigh River in Gilan province, BabolRoud River in Maz&eran province & GorganRoud River in Golestan province). Genomic DNA from 121 specimens was extracted from fin tissue by phenol-Chlorophorm method & PCR reaction was accomplished with 17 microsatellite primers, out of 17 microsatellite primers 14 loci were amplified, in which 10 of them were amplified with reasonable polymorphism & 4 were monomorphism. Totally 302 alleles were identified on average 7.5 Observed & expected heterozygosity averages were 0.80 & 0.77 respectively. Most cases significantly deviated from Hardy-Weinberg equilibrium (p 0.01). The estimation of Fst (p 0.01) revealed significant population structuring & estimate four population of Vimba vimba persa is identified in the Caspian Sea. These studies were to apply & develop population genetic approaches to assist conservation, sustainable harvest & restocking of these populations.
    Keywords: Biology ; Iran ; Caspian Sea ; Gilan province ; Mazandaran Province ; Golestan Province ; Anzali Lagoon ; Vimba vimba persa ; Population ; Genetic ; Microsatellite ; DNA ; Genomic ; Specimens ; PCR ; Equilibrium ; Restocking
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 61
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