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  • 1
    Publication Date: 2021-05-19
    Description: The effects dietary supplementation with Aloe vera extract (AE) on the immunity responses and hematological parameters of rainbow trout (Oncorhynchus mykiss) fry were evaluated in eight weeks trial. 600 rainbow trout (O. mykiss) fry with an average initial body weight of 13±0.05g were randomly allocated into two treatment groups including placebo-treated group (control) and A. vera extract-treated group, each with three replicates. The fish were hand-fed once a day with diet medicated AE or placebo (70 % lactose, 10 % starch and 20 % talc) at a rate of 1% of feed weight in the first feeding for 8 weeks. At the end of the identical every two weeks (2, 4, 6, and 8 weeks) 24 h after feeding, some of immunological and hematological parameters were analyzed. The results showed that serum total protein, albumin and globulin, respiratory burst activity, phagocytic activity and serum lysozyme activity vary among the two treatment groups which were found to be higher in the AE-treated group (p〈0.05). However, there were no significant differences in none of hematological parameters between two groups. It was concluded that supplementation of AE at a rate of 1% in feed registered higher immunological responses in compared to placebo group. Therefore, supplementation of AE in fish diet would enhance the immunity responses in fish. It may use in fish diets particularly at time of outbreaks.
    Description: Published
    Keywords: Immune responses ; Aloe vera ; Oncorhynchus mykiss ; Herbal medicine
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.884-896
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  • 2
    Publication Date: 2021-05-19
    Description: This study aimed to investigate the effects of 5- parameter dissolved oxygen, PH, nitrite, nitrate and temperature of the water on Streptococcosis incidence in two groups of fry and grower fish. Research was performed at west Mazandaran province -Tonkabon region in 12 rainbow trout selected farms. Research conducted in 3 farms with well source water , 8 farms with water source of the river of Dohezar and one farm with water source of the river of Azarood, during 12 consecutive months.From 1390/04/01 to 1391/04/01, once time each month , and in each time 10 fish randomly sampled, inlet water were sampled simultaneously. Of 1350 sampled fish 607 fish with an average weight 22.04 gr , average length 12.59 cm were in fry category and 743 fish with an average weight 156.25 gr, average length 23.32 cm in were grower category. Streptococcosis observed only in grower category. Of 72 fish with clinical signs of the disease, 14 numbers were positive Streptococcosis (19.44%) and 58 numbers were negative Streptococcosis(80.55%). Three fish from grower category has not any clinical signs of disease and seemed to be healthy but were positive Streptococcosis in examinations (0.22% of total fish sampled. Fish with clinical signs of the disease but negative Streptococcosis were of at least 55.1 gr and at most weight 417 gr respectively. The results showed that 47.07% cases of Streptococcosis happened at 15.6 °C water temperature, 35.29% at 16.98 °C and 17.64% cases happened at 18.04 °C so that 100% of Streptococcosis cases was observed at the average temperature of 16.99 °C. In addition, the survey results show that despite relatively high levels of nitrite in source water of farms from wells, nitrite does not effect on the disease incidence. It seems that an optimal level of dissolved oxygen in water is effective in reducing the effectiveness of nitrite in this disease. According to equation coefficients logit model is as follows: According to Logit model, it seems that for every degree change in temperature and pH of water, morbidity change will diminish 0.37 % and 1.74 % respectively. 80.56% of fish sampled that had the clinical symptoms, was ranging from an average weight of 5.1 gr (fry) and 417 gr (grower fish), that despite having clinical signs of the disease were negative Streptococcosis. Isolation of Staphylococcus bacteria as well as Gram-negative bacilli from fish with clinical symptoms similar to the symptoms of Streptococcosis, may indicate the involvement of other pathogens in fish clinical signs.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Streptococcusis ; Risk factors ; Cold water fish ; Survey ; Rainbow trout ; Dissolved oxygen ; pH ; Nitrite ; Clinical signs ; Temperature ; Pathogen
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 60pp.
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  • 3
    Publication Date: 2021-05-19
    Description: Cheshmehkileh River and adjacent mountainous streams, play a strategic role as a historical axis for anthropogenic civilization, human welfare also habitat and migration pathway of commercial – biologic valuable fishes e.g. Caspian trout, Caspian kuttum, members of Cyprinidae family in south Caspian Sea drainage. Treats such as overfishing of Caspian trout and Red spotted trout stocks in mountainous headwaters, barriers construction and manipulations those are out of river carrying capacity developed by human activities, affected normal function of river as well. Sand mining big factories establishment next to the river, legal and illegal trade of river sediments, direct entry of Tonekabon landfill leakage into the river, development of Rainbow trout farms since 3 decades and huge effluents into the river containing dead fish and types of solids, escapement of cultured Rainbow trouts into the river, … are major minimum factors which needs basic information for integrating inclusively drainage management system. Cheshmehkileh River contains Headwaters of Dohezar (Daryasar & Nusha), Sehezar and Valamroud rivers during 13 monthly sampling phases between September 2009 and October 2010 based on macrozoobenthoses investigations by EPT, EPT/C EPA protocols, measurements of nominated physic-chemical and microbiologic parameters. Probability of Rainbow trouts escapement and invasion, existence, nutrition in Cheshmehkileh environment indeed investigated. Data analysis explained significant differences (P〈0.05) between groups of measured parameters in different sampling stations. Dendogram of clustered analysis based on consolidation of major biologic/ physic-chemical and microbiologic parameters, separated stations No. 1, 3, 2, 4 in one group and remained classified in different groups. Station 8 and 9 similarly separated which expressed general similarities according to Sehezar river environment which were differs in comparison with other stations. Station 11 separated according to its natural quality of water and environment. Similarities between station 10 to Sehezar river stations 8 and 9 expressed general influence of Sehezar River more than Dohezar River in Cheshmehkileh condition especially in station No. 10. High scores of EPT and EPT/C indices in upstream stations 1, 3 and 8 also low score of indices in stations 7, 13 and 6 expressed levels of environment quality between these groups of stations. Maximum average biomass of macroinvertebrates belongs to Trichoptera order in Cheshmehkileh River. Significant decrease of biomass in stations 11, 12 and 13 in comparison with other stations stated environment degradation in mentioned stations relevant to excessive sand mining as well. Pollution resistant groups of invertebrates significantly increased in downstreams against upstream stations. Also disappearing of Plecoptera order in station No. 7, 9, 10 and 13 stated low quality of environment in comparison with upstream stations. Confirmation of effects quality and quantity for point and non-point sources of imported pollutants require specific management considerations in order to present exploitations, pollutants control and emergencies for river monitoring in forthcoming years.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: River ; Pollution ; Aquaculture ; EPTC ; Assessment ; Macroinvertebrates ; Chemistry ; Microbiology
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 138pp.
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  • 4
    Publication Date: 2021-05-19
    Description: Silkworm pupae meal is a non-conventional animal protein feedstuff. It is the by-product after the silk thread has been wound off from the cocoon. To investigate the effects of animal protein on growth and survival of rainbow trout (Oncorhynchus mykiss), a sixty-day feeding experiment was conducted. Four replacement levels (0, 5, 10 and 15 percent) of silkworm pupae meal were compared using a completely random design. We used 360 juvenile rainbow trout (average weight 55 plus or minus 3.42g) divided into 4 groups and 3 replications, each containing 30 trout for 60 days. Sampling for nutritional effects was carried out every 10 days and at the end of the experiment, weight gain, feed conversion ratio, specific growth rate, protein and efficiency ratio were compared which showed no significant differences (P〉0.05) among the treatments. Total length and survival rate were not significantly affected in the treatment groups. The highest percentage of carcass protein and the lowest percentage of carcass fat belonged to the control treatment. Our findings showed that silkworm pupae meal could replace 15% of fish meal diet in rainbow trout culture.
    Description: Published
    Keywords: Oncorhynchus mykiss ; Rainbow trout ; Growth rate ; Growth ; Fish meal ; Carcasses ; Pupae ; Proteins ; Aquaculture ; Freshwater
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.77-84
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  • 5
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25617 | 18721 | 2018-10-07 16:24:05 | 25617 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The project is funded by the CASPECO Project which is the Third Phase of the GEF supported project in the Caspian Sea region titled “The Caspian Sea: Restoring Depleted Fisheries and Consolidation of a Permanent Regional Environmental Governance Framework”, and implemented by the Iranian Fisheries Research Organization (IFRO) in partnership with four Coastal Research Centers affiliated to IFRO, Coldwater Fishes Research Centre (CFRC).The project's objective is to improve the conservation and restoration of Caspian Salmon (Salmo trutta caspius) stocks. In accordance with IUCN criteria Caspian Salmon is placed in the red list of endangered fish. In the recent decades the destruction of habitats for juveniles, reduction of genetic diversity and fish immune system were main causes of the Caspian salmon decline. Numbers of public meetings were held with participation of fishing communities’ representatives, experts, relevant local authorities and experts of the IFRO and Iranian Fisheries Organization (IFO), Iran Environment Protection Organization (IEPO), Water Resource Management (Ministry of Energy) and University senior professors, in order to create interaction and understanding of the project objectives. Educational awareness and extension program were conducted with the involvement of fisheries communities, private sector and NGO associations. The results of the project suggests that cross-sectoral coordination for sustainable fisheries can be achieved through building partnerships and enhancing communication between the environment, private, fisheries sectors and academia. Cross-sectoral cooperation through this project improved public awareness on growing risks facing Caspian Salmon from different sources including local sources (illegal fishing and over-fishing, urban wastewater, environment destruction, industrial pollutants, pollution of air and water resources), new emerging and invasive organisms, climate change, implications of the Caspian salmon population decline on economic and social affairs of the people who depend on them for their livelihood and food security and their role in fish stock protection. The project, through its capacity building, extension and educational programs and efforts to link science to management, could translate scientific findings into an action agenda for managers and policy makers that can bring an improvement in the future of the Caspian Sea salmon stock.
    Keywords: Management ; Iran ; Caspian Sea ; Conservation ; Salmo trutta caspius ; Caspian salmon ; Fisheries ; IFRO ; Juvenile ; Genetic diversity ; Immune system ; Illegal fishing ; Pollution
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 194
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  • 6
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25792 | 18721 | 2018-10-13 08:54:26 | 25792 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Cheshmehkileh River and adjacent mountainous streams, play a strategic role as a historical axis for anthropogenic civilization, human welfare also habitat and migration pathway of commercial – biologic valuable fishes e.g. Caspian trout, Caspian kuttum, members of Cyprinidae family in south Caspian Sea drainage. Treats such as overfishing of Caspian trout and Red spotted trout stocks in mountainous headwaters, barriers construction and manipulations those are out of river carrying capacity developed by human activities, affected normal function of river as well. Sand mining big factories establishment next to the river, legal and illegal trade of river sediments, direct entry of Tonekabon landfill leakage into the river, development of Rainbow trout farms since 3 decades and huge effluents into the river containing dead fish and types of solids, escapement of cultured Rainbow trouts into the river, … are major minimum factors which needs basic information for integrating inclusively drainage management system. Cheshmehkileh River contains Headwaters of Dohezar (Daryasar & Nusha), Sehezar and Valamroud rivers during 13 monthly sampling phases between September 2009 and October 2010 based on macrozoobenthoses investigations by EPT, EPT/C EPA protocols, measurements of nominated physic-chemical and microbiologic parameters. Probability of Rainbow trouts escapement and invasion, existence, nutrition in Cheshmehkileh environment indeed investigated. Data analysis explained significant differences (P〈0.05) between groups of measured parameters in different sampling stations. Dendogram of clustered analysis based on consolidation of major biologic/ physic-chemical and microbiologic parameters, separated stations No. 1, 3, 2, 4 in one group and remained classified in different groups. Station 8 and 9 similarly separated which expressed general similarities according to Sehezar river environment which were differs in comparison with other stations. Station 11 separated according to its natural quality of water and environment. Similarities between station 10 to Sehezar river stations 8 and 9 expressed general influence of Sehezar River more than Dohezar River in Cheshmehkileh condition especially in station No. 10. High scores of EPT and EPT/C indices in upstream stations 1, 3 and 8 also low score of indices in stations 7, 13 and 6 expressed levels of environment quality between these groups of stations. Maximum average biomass of macroinvertebrates belongs to Trichoptera order in Cheshmehkileh River. Significant decrease of biomass in stations 11, 12 and 13 in comparison with other stations stated environment degradation in mentioned stations relevant to excessive sand mining as well. Pollution resistant groups of invertebrates significantly increased in downstreams against upstream stations. Also disappearing of Plecoptera order in station No. 7, 9, 10 and 13 stated low quality of environment in comparison with upstream stations. Confirmation of effects quality and quantity for point and non-point sources of imported pollutants require specific management considerations in order to present exploitations, pollutants control and emergencies for river monitoring in forthcoming years.
    Keywords: Aquaculture ; Management ; Iran ; Tonekabon ; Cheshmehkileh ; River ; Pollution ; Aquaculture ; EPTC ; Assessment ; Macroinvertebrates ; Chemistry ; Microbiology
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 138
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  • 7
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    In:  http://aquaticcommons.org/id/eprint/23856 | 18721 | 2018-08-01 04:42:02 | 23856 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-15
    Description: Silkworm pupae meal is a non-conventional animal protein feedstuff. It is the by-product after the silk thread has been wound off from the cocoon. To investigate the effects of animal protein on growth and survival of rainbow trout (Oncorhynchus mykiss), a sixty-day feeding experiment was conducted. Four replacement levels (0, 5, 10 and 15 percent) of silkworm pupae meal were compared using a completely random design. We used 360 juvenile rainbow trout (average weight 55±3.42g) divided into 4 groups and 3 replications, each containing 30 trout for 60 days. Sampling for nutritional effects was carried out every 10 days and at the end of the experiment, weight gain, feed conversion ratio, specific growth rate, protein and efficiency ratio were compared which showed no significant differences (P〉0.05) among the treatments. Total length and survival rate were not significantly affected in the treatment groups. The highest percentage of carcass protein and the lowest percentage of carcass fat belonged to the control treatment. Our findings showed that silkworm pupae meal could replace 15% of fish meal diet in rainbow trout culture.
    Keywords: Chemistry ; Fisheries ; Nutrition ; Feed conversion ratio ; Protein ; Growth ; Rainbow trout ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 77-84
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  • 8
    Publication Date: 2021-07-16
    Description: This study aimed to investigate the effects of 5- parameter dissolved oxygen, PH, nitrite, nitrate and temperature of the water on Streptococcosis incidence in two groups of fry and grower fish. Research was performed at west Mazandaran province -Tonkabon region in 12 rainbow trout selected farms. Research conducted in 3 farms with well source water, 8 farms with water source of the river of Dohezar and one farm with water source of the river of Azarood, during 12 consecutive months. From 1390.04.01 to 1391.04.01, once time each month, and in each time 10 fish randomly sampled, inlet water were sampled simultaneously Of 1350 sampled fish 607 fish with an average weight 22.04 gr, average length 12.59 cm were in fry category and 743 fish with an average weight 156.25 gr, average length 23.32 cm in were grower category. Streptococcosis observed only in grower category Of 72 fish with clinical signs of the disease, 14 numbers were positive Streptococcosis (19.44%) and 58 numbers were negative Streptococcosis (80.55%). Three fish from grower category has not any clinical signs of disease and seemed to be healthy but were positive Streptococcosis in examinations (0.22% of total fish sampled. Fish with clinical signs of the disease but negative Streptococcosis were of at least 55.1 gr and at most weight 417 gr respectively. The results showed that 47.07% cases of Streptococcosis happened at 15.6°C water temperature, 35.29% at 16.98°C and 17.64% cases happened at 18.04°C so that 100% of Streptococcosis cases was observed at the average temperature of 16.99°C. In addition, the survey results show that despite relatively high levels of nitrite in source water of farms from wells, nitrite does not effect on the disease incidence. It seems that an optimal level of dissolved oxygen in water is effective in reducing the effectiveness of nitrite in this disease. According to equation coefficients logit model is as follows: According to Logit model, it seems that for every degree change in temperature and pH of water, morbidity change will diminish 0.37 % and 1.74 % respectively. 80.56% of fish sampled that had the clinical symptoms, was ranging from an average weight of 5.1 gr (fry) and 417 gr (grower fish), that despite having clinical signs of the disease were negative Streptococcosis. Isolation of Staphylococcus bacteria as well as Gram-negative bacilli from fish with clinical symptoms similar to the symptoms of Streptococcosis, may indicate the involvement of other pathogens in fish clinical signs.
    Keywords: Ecology ; Iran ; Mazandaran Province ; Tonkabon ; Streptococcusis ; Risk factors ; Cold water fish ; Survey ; Rainbow trout ; Dissolved oxygen ; pH ; Nitrite ; Clinical signs ; Temperature ; Pathogen
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 60
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  • 9
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    In:  http://aquaticcommons.org/id/eprint/21816 | 18721 | 2018-01-08 10:04:44 | 21816 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-02
    Description: The changes in density and biomass of macrobenthos were seasonally investigated from winter 2005 till fall 2006 in southern Caspian Sea. Samples were taken with a 225cm 2 Van Veen grab from depths of 7 and 20 meters along two transects: Line 8 area (western part) and Radio Darya area (eastern part). Totally, 5 classes of macrobenthos were identified of which the most abundant populations were Polychaeta 68.7%, Oligochaeta 14.6%, Bivalvia 8.5%, Crustacea 8.2% and Gastropoda 0.08%. The maximum density (4251.8 1419 (ME SE) ind/m2) of macrobenthos was observed during summer in the depth of 20m of Radio Darya area, while the minimum density (725.9 64.5 (ME SE) ind/m2) was observed during spring in the depth of 7m in the same area. Between the two studied depths, the mean annual density in 7m depth (2266.7 350.3 (ME SE)ind/m2), was higher from 20m depth (1977.8 290.1(ME SE)ind/m2). The maximum biomass (475 243.8 (ME SE)g/m2), of macrobenthos was observed during autumn in the depth of 20 meter of Radio Darya area, while the minimum biomass (0.3 0.1 (ME SE)g/m2), was observed during winter in the depth of 7m in the same area. Between the two studied depths, the mean annual biomass in 20m depth (184.8 24.6 (ME SE)g/m2), was higher from 7m depth (73.7 24.6 (ME SE)g/m2). Generally speaking, the eastern part of Chalus coast had more density and biomass compared with the western part.
    Keywords: Biology ; Ecology ; Biomass ; Macrobenthos ; Ecology ; Caspian Sea ; Iran ; Chalus Shore ; density ; biomass
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 147-152
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  • 10
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    In:  http://aquaticcommons.org/id/eprint/23126 | 18721 | 2018-06-18 17:08:26 | 23126 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-13
    Description: The effects dietary supplementation with Aloe vera extract (AE) on the immunity responses and hematological parameters of rainbow trout (Oncorhynchus mykiss) fry were evaluated in eight weeks trial. 600 rainbow trout (O. mykiss) fry with an average initial body weight of 13±0.05g were randomly allocated into two treatment groups including placebo-treated group (control) and A. vera extract-treated group, each with three replicates. The fish were hand-fed once a day with diet medicated AE or placebo (70% lactose, 10% starch and 20% talc) at a rate of 1% of feed weight in the first feeding for 8 weeks. At the end of the identical every two weeks (2, 4, 6, and 8 weeks) 24 h after feeding, some of immunological and hematological parameters were analyzed. The results showed that serum total protein, albumin and globulin, respiratory burst activity, phagocytic activity and serum lysozyme activity vary among the two treatment groups which were found to be higher in the AE-treated group (p〈0.05). However, there were no significant differences in none of hematological parameters between two groups. It was concluded that supplementation of AE at a rate of 1% in feed registered higher immunological responses in compared to placebo group. Therefore, supplementation of AE in fish diet would enhance the immunity responses in fish. It may use in fish diets particularly at time of outbreaks.
    Keywords: Aquaculture ; Biology ; Fisheries ; Immune responses ; Aloe vera ; Oncorhynchus mykiss ; Herbal medicine ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 884-896
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