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  • 1
    Publication Date: 2021-05-19
    Description: Red tide event a product of micro algae abundance has elevated in last few years. Loss of aquatic life due to HAB, and its effect on human health, has caused tremendous damage in the world. In this research the effect of (1, 2, 3, 9, and 30) ×10^3 Cells ml^-1 densities of Cochlodinium polykrikoides on survival and histopathological effect on hepatopancreas and gills of Litopenaeus vannamei postlarvae (PLs) and juvenile stages has been investigated. Results showed 100% survival with (1, 2, and 3) ×10^3 Cells ml-1 densities but accumulative mortality in 9×10^3 Cells ml^-1 density were 1.33% and 2.22%, in PLs and juvenile stages respectively, and there is no significant difference between control and treatment groups. accumulative mortality in 3×10^4 Cells ml^-1 density was 4.44% and 17.8% in PLs and juveniles respectively which have significant different with control groups. According to histopathological investigation in this density the infiltration of blood cells in connective tissue of hepatopancreatic tubules and gills lamellae of PLs and juvenile stages were low but high in 3×10^4 Cells ml^-1. The intensity of infiltration in juvenile stages was higher than PLs, which indicate incensement of hepatopancreatic and gills blood cells in higher densities of this phytoplankton. The end result of the study indicates that in high densities of C. polykrikoides survival rate in PLs and juveniles decreased and according to histopathological study the most important possible factor in shrimp mortality when exposure to C. polykrikoides was settlement of phytoplankton on gills lamellae and preventing oxygen exchange of shrimp.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Cochlodinium polykrikoides ; Litopenaeus vannamei ; Bloom ; Histopathological ; Survival ; Investigation
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 78pp.
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  • 2
    Publication Date: 2021-05-19
    Description: White Spot Disease (WSD) is a important disease due to economic impacts in shrimp industries. Spreading of this disease in shrimp farms can caused a 100% mortality during 3-10 days. Therefore control of this disease is a strategy in shrimp industry. Vaccination is a way to control of WSD. In several years ago during a project several type vaccine of this virus by association of Atomic Energy Organization is produced that among of them the virus inactivated by GAMA radiation had better results in laboratory. To test of this vaccine in field, a research pilot was carried out. Initially 20000 shrimp napliies were obtained from one of commercial hatchery in Bushehr province, then devided to two groups vaccinated and unvaccinated. The vaccinated group also devided . 26and PL12 , group B vaccinated at PL15and PL 5to two groups, group A that vaccinated at postlarva e (PL) Rrsults showed that difference of growth performance and survival rate between vaccinated and unvaccinated without challenge with WSV after 80 days is not significantly (P〈0.05). but survival rate in vaccinated groups after challenge with WSV was significantly (P〈0.05) further the unvaccinated group. Also the results showed survival rate of group B after challenge with WSV is better than group A and this difference was significantly (P〈0.05). this study showed vaccination of shrimp postlarvae with GAMA radiation vaccine can control of shrimp mortality in incidence of WSD in farms.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Litopenaeus vannamei ; WSSV ; Gamma radiation ; Vaccination ; Y-ray ; White spot disease ; Shrimp ; Nauplii ; Growth ; Survival rate ; Mortality
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 65pp.
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  • 3
    Publication Date: 2021-05-19
    Description: After the outbreak of various viral diseases, specifically white spot syndrome (WSS) in farmed Litopenaeus vannamei in the past few years, this non-native shrimp species in Iran requires a generator-builder with specific a pathogen-free approach. This Research was done by the Iran Shrimp Research Center (ISRC) to access accurate information regarding specific pathogen-free shrimp production and avoid using shrimp broodstock production through foreign investors. This research was part of the molecular viral study. For this trial, selected shrimps in farms were sampled and screened for main viruses (OIE list). Shrimps without infection were brought to the quarantine system for one month and at the end of the quarantine they were screened again. In addition, in winter spending, before and after spawning, it’s offspring in F1 and F2 were also screened for viral pathogens by IQ2000 kit at the Iran Shrimp Research Center lab and Iranian veterinary organization lab. All shrimp products such as fresh and consumed food were tested for OIE list at all stages during project performance and the result of tests were negative. Sequenced and molecular tests with specific primers were used to determine the presence of infected samples carrying the virus in any of the products, such as shrimp and consumed food, the infected samples. The result of all the tests during last three years, shows that all the shrimp and their food products were pathogen-free and safe to use.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Shrim ; SPF ; Virus ; White Spot Syndrome Virus ; WSS ; Spawning ; Molecular
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 48pp.
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  • 4
    Publication Date: 2021-05-19
    Description: At present, the aquaculture industry to provide proper instructions in the field of health management, including production of Specific Pathogen Free shrimp (SPF), require sensitive and reliable methods for the detection and identification of pathogenic microorganisms. Molecular methods which used in the detection of microorganisms have a high discriminatory power in the taxonomy and in relation to libraries in the world. On the other hand, the accurate identification of microorganisms, providing the genetic data bank of shrimp pathogens and maintenance of these strains is the step to promote further research on the mechanisms of pathogenesis of pathogens, diagnosis, treatment, prevention of disease, identify indigenous production kits, diagnosis re emerging and emerging diseases and their origin. Therefore, in this project, by using ribotyping technique, native isolated pathogenic bacteria and fungi were identified and recorded in the gene bank database center. During sampling of shrimp and water of Specific Pathogen Free shrimp center, 40 bacterial strains were isolated, which 8 of them had the most frequency and identification based on 16S rDNA sequencing was performed. Bacteria identified are: Vibrio nigripulchritudo strain IS013(GenBank:KP843725), Vibrio brasiliensis strain IS014 (GenBank:KR186076), Vibrio rotiferianus strain IS015 (GenBank:KR186077), Vibrio azureus strain IS012 (GenBank:KJ018724.1), Vibrio owensii strain IS016 (GenBank:KR186078), Agarivorans gilvus strain IS017 (GenBank:KR186079), Vibrio brasiliensis IS018 (GenBank:KR186080) and Vibrio alginolyticus strain IS019 (GenBank:1817854), which were recorded in The World Bank genes. In this study fungal isolates were not detected.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Bacteria ; Fungi ; Ribotyping ; 16S rDNA ; Shrimp ; Specific pathogen free ; Vibrio rotiferianus ; Vibrio owensii ; Vibrio brasiliensis ; Vibrio azureus ; Agarivorans gilvus ; Vibrio alginolyticus ; GenBank
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 64pp.
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  • 5
    Publication Date: 2021-05-19
    Description: DNA barcode is a short, standard well known sequence of cytochrome oxidase І gene. By using this DNA sequence can be realized that each animal, plant or fungus belongs to which species. in this research, samples were collected from imported cultured shrimp Litopenaeus vannamei and and 6 Persian Gulf and Oman Sea shrimp species which classified based on traditional systematically as: Penaeus semisulcatus, Fenneropenaeus merguiensis, Metapenaeus affinis, Parapenaeopsis Stylifera and Fenneropenaeus indicus. After examination of DNA barcode sequence, molecular and bioinformatics operations of each sequence in the Consortium for the Barcode of Life (CBOL) and National Center for Biotechnology Information (NCBI), phylogenetic analysis of each sample was determined and similarity of each sample with NCBI and CBOL database was checked and the closest species to each sample were specified. According to the results different samples of L. vannamei, . banded P.semisulcatus, F. merguiensis and F. indicus have more than 97% similarity to the same species of other countries. non banded P.semisulcatus had 80.07% similarity to banded P.semisulcatus, M. affinis samples had 90.3% similarity to Metapenaeus ensis and Parap. Stylifera had 93.44% similarity to Parapenaeopsis coromandelica in the CBOL. This funding confirmed the need for further investigation and possible announcement of new species.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: DNA barcode ; Shrimp ; Litopenaeus vannamei ; Penaeus semisulcatus ; Fenneropenaeus merguiensis ; Metapenaeus affinis ; Parapenaeopsis stylifera ; Fenneropenaeus indicus
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 72pp.
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  • 6
    Publication Date: 2021-05-19
    Description: This study is a part of the national and technology master plan entitled "Attain to technical knowledge of specific pathogen free shrimp production and cut off to dependence on foreign products". The goals of this work were to assess and analysis the risk factors of production of specific pathogen free Litopenaeus vannamei shrimp in its all stages (broodstocking, laravl production, shrimp farming, feeding and water quality), determining the pattern of outcomes monitoring, management and control of outcomes from April 2012 to September 2015 in Bandargah research station and Persian Gulf SPF Shrimp research station and their environment, belonging to Shrimp Research Center, located in Bushehr. Identification and screening of risk factors have been done based on Environment Aspect and Effect Analysis method, (EA)2, using a fine statement questionnaire and quantification of risks. The number of identified risk factors in Bandargah research station was 15 while it was 13 in Persian Gulf SPF Shrimp research station. The more number of risk factors in Bandargah station in respect to its number in Persian Gulf station is might due to lower level of biosecurity, physical structure and aging of Bandargah station and existence of two possible source of contamination, the Bushehr nuclear power plant pollutants and Bandargah fishing harbor, near to the station. On the basis of results, the maximum values of APN in Bandargah station was 42 while it was 30 in Persian Gulf station. These results revealed that the level of risk in Bandargah station is lower than that in Persian Gulf station. The most probable risks in both studied research stations are related to transmission of pathogens in shrimp feeding and water intake pollution.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Environmental risk assessment ; Shrimp ; Litopenaeus vannamei ; Specific pathogen free
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 80pp.
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  • 7
    Publication Date: 2021-05-19
    Description: Nowadays, white spot virus disease is serious threat for breeding and culture industry of shrimp. In this study was increasing resistance of shrimp against white spot virus by using modern methods such as shrimp vaccination with inactivated viruses and recombinant proteins. The aim of this study were determine the growth and survival rates vaccinated and non-vaccinated of Litopenaeus vannamei (5 to 15 and 12 to 26 day), that were challenged with white spot virus in 40 and 60 day rearing. This study consisted two separate groups were vaccinated and non-vaccinated with 11 treatments experimental and each of replicate was stocked 1000 pieces by post larva of 5 to15 and 12 to 26 day. After vaccination, two groups of post larvae exposed to the white spot virus at 40 and 60 day, one groups no exposure to the virus. Samplings were randomly of shrimp in 40, 60 and 80 days 10 pieces each of treatment experimental and measured mean of weight and length. Also, number of deaths was recorded at morning and evening daily and calculated survival rate at the end of study. The results showed growth rate of post larvae vaccinated (5 to15 day) which exposed to white spot virus at 40 and 60 was significantly lower than non-vaccinated of post larval, while the growth rate of post larval exposed to virus in 60 day was significantly increased. On the other hand, growth rate of post larval vaccination (12 to 26 day) exposed to virus in 60 day compared with post larval vaccination (5 to 15 and 12 to 26) exposed to virus in 40 and 60 days was significantly increased. Hence, growth rate was significantly increased in post larval vaccinated (5 to 15 and 12 to 26) which non-exposed to virus. Although the survival rate was post larval vaccinated (12 to 26 days) exposed to virus Post larvae in 60 day higher than post larval were exposed to virus in 40 and 60, but no significant differences were observed. However, relative mortality of post larval vaccination in 12 to 26 day compared with post larval vaccination in 5 to 15 days exposed to virus were significantly lower. Considering growth and survival index was improved of post larval vaccination can be concluded that the optimum age for vaccination against white spot virus of L.vannamei was 12 to 26 day.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: White spot virus ; Litopenaeus vannamei ; Vaccination ; Growth ; Survival rate
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 45pp.
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  • 8
    Publication Date: 2021-05-19
    Description: In order to continue improving the quality of Iranian fisheries science research institute's laboratories activities, For the first time in the years 2014-2015, a project with the subject of ―risk assessment of laboratories‖ conducted in Iran Shrimp Research Center, to determine the risk factors, potential risks and provide action plans to prevent the occurrence of any risks and increasing awareness of staff to safety issues. This project was done in eight laboratories which were named: Plankton, Pathology, Molecular genetics (extraction and elechtrophoresis), Pollutants, Marine physical chemistry, Sediment and benthos, Aquatic animal biometry and Microbiology with Failure Mode and Effects Analysis (FMEA) methods. The assessment process was designed and implemented in two phases: at the first phase, the current situation of safety indexes, risks identification and distance to laboratory safety parameters included: working environment, physical factors, test methods and the human factors were done, then risk assessment and risk management of identified risks were followed by check list forms. At the second phase, based on the findings ,control measures and safety guidelines were done and provided. According to the findings, the highest risk score related to Microbiology laboratory and pollutant laboratory with RPN 210, at the next level Marine physical chemistry laboratory with risk number 150 and respectively Molecular genetics laboratory (extraction), Sediment and benthos, Pathology, Molecular genetics (electrophoresis), Plankton and Aquatic animal biometry laboratories were existed. After control measures, the risk number of laboratories decreased as follows: Microbiology to 150, Pollutants to 120, Marine physical chemistry laboratory to 80, Molecular genetics (extraction) and Sediment and Benthos Laboratories to 48, Pathology decrease to 18, Molecular genetics (electrophoresis), Plankton and Aquatic animal biometry laboratories decreased to 8. It is hoped that by promoting safety knowledge of laboratory staff and providing financial can be able to reduce risk assessment number of all laboratories to very low rank.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Assessment ; Risk ; FMEA ; Laboratory ; Marine physical chemistry laboratory ; Molecular genetics ; Sediment and Benthos Laboratories
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 84pp.
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  • 9
    Publication Date: 2021-05-19
    Description: Aquaculture is the fastest growing food industry in the world. Shrimp culture industry is also part of it, unfortunately, like other marine animal culture economic losses caused by the disease has been one of the major challenges of this industry. The major cause of mortality in shrimp hatcheries and rearing centers is related to water quality and the presence of pathogenic bacteria and parasites. These are common opportunistic microorganisms in the hatchery, rearing centers, flora and living food but poor conditions of culture are caused diseases. Since the development of aquaculture in the countries need health management, one of the important additional rings in the shrimp strategic plan is specific pathogen free shrimp production, which has been addressed in this plan. Specific pathogen free shrimp define as the shrimps which are free of the specific pathogens listed in world organization for animal health (OIE). These factors should be conclusively diagnosed and can be isolated from shrimp hatcheries and culture system. Therefore in this project screening and surveillance of shrimp in several generations according to the list of OIE were done and they were monitored for of bacterial pathogens (Necrosis Hepatopancreas Bacteria) and parasites (Microsporidian and Gregarins). At total 756 pieces of shrimp, 6 sample of dry food and 97 samples of live foods were controlled and tested. 1.35 percent of live foods were positive for NHPB and 5.6 percent of pre broodstocks have eppicommensal and microsporidia which were disposed in quarantine phase. Because of biosecurity and surveillance system establishment, there were no bacterial or parasitic isolation or diagnosis during SPF shrimp production.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Litopenaeus vannamei ; SPF ; Bacteria ; Parasite ; Aquaculture
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 60pp.
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  • 10
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25553 | 18721 | 2018-10-05 16:16:38 | 25553 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Nowadays, white spot virus disease is serious threat for breeding and culture industry of shrimp. In this study was increasing resistance of shrimp against white spot virus by using modern methods such as shrimp vaccination with inactivated viruses and recombinant proteins. The aim of this study were determine the growth and survival rates vaccinated and non-vaccinated of Litopenaeus vannamei (5 to 15 and 12 to 26 day), that were challenged with white spot virus in 40 and 60 day rearing. This study consisted two separate groups were vaccinated and non-vaccinated with 11 treatments experimental and each of replicate was stocked 1000 pieces by post larva of 5 to15 and 12 to 26 day. After vaccination, two groups of post larvae exposed to the white spot virus at 40 and 60 day, one groups no exposure to the virus. Samplings were randomly of shrimp in 40, 60 and 80 days 10 pieces each of treatment experimental and measured mean of weight and length. Also, number of deaths was recorded at morning and evening daily and calculated survival rate at the end of study. The results showed growth rate of post larvae vaccinated (5 to15 day) which exposed to white spot virus at 40 and 60 was significantly lower than non-vaccinated of post larval, while the growth rate of post larval exposed to virus in 60 day was significantly increased. On the other hand, growth rate of post larval vaccination (12 to 26 day) exposed to virus in 60 day compared with post larval vaccination (5 to 15 and 12 to 26) exposed to virus in 40 and 60 days was significantly increased. Hence, growth rate was significantly increased in post larval vaccinated (5 to 15 and 12 to 26) which non-exposed to virus. Although the survival rate was post larval vaccinated (12 to 26 days) exposed to virus Post larvae in 60 day higher than post larval were exposed to virus in 40 and 60, but no significant differences were observed. However, relative mortality of post larval vaccination in 12 to 26 day compared with post larval vaccination in 5 to 15 days exposed to virus were significantly lower. Considering growth and survival index was improved of post larval vaccination can be concluded that the optimum age for vaccination against white spot virus of L.vannamei was 12 to 26 day.
    Keywords: Aquaculture ; Iran ; White spot virus ; Litopenaeus vannamei ; Vaccination ; Growth ; Survival rate
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 45
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