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  • 1
  • 2
    Publication Date: 2015-11-01
    Print ISSN: 1050-4648
    Electronic ISSN: 1095-9947
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Published by Elsevier
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  • 3
    Publication Date: 2014-01-01
    Print ISSN: 1050-4648
    Electronic ISSN: 1095-9947
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Published by Elsevier
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  • 4
    Publication Date: 2016-08-01
    Print ISSN: 1050-4648
    Electronic ISSN: 1095-9947
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Published by Elsevier
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  • 5
    Publication Date: 2021-05-19
    Description: S. iniae is an important pathogen in both marin animals and humans, causing systemic infections in these hosts. The symptoms of infections are very similar to symptoms of infections caused by human¬specific streptococcal pathogens. The enzyme phosphoglucomutase (PGM) has recently been discovered to play an important role in polysaccharide capsule production and virulence in s. iniae. We aim to isolate S. iniae and cloning phosphoglucomutase gene. S. iniae grew on sheep blood agar as mucoid and beta- hemolytic colonies after 24 h of incubation at 37C. Then confirmed it by sensitive and rapid method of PCR. The pgm gene was amplified successfully and cloned in pTZ57R cloning vector.The recombinant plasmid was sub cloned into pETDuet-l expression vector by rectriction enzymes and confirmed by PCR . Although S. iniae is a pathogen of economic importance, relatively little is known regarding mechanisms of its pathogenesis and few specific virulence determinants have been established. These include the enzyme phosphoglucomutase which contributes to cell wall integrity and resistance to antimicrobial peptides. Thus, this enzyme is the best choice to product the vaccine and more investigations.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Streptococcusis ; Gene ; Streptococcus iniae ; Gene Bank ; S. iniae ; Pathogen ; Enzyme ; Phosphoglucomutase ; PCR
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 33pp.
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  • 6
    Publication Date: 2021-05-19
    Description: At the present study, the water quality parameters monthly, environmental pollutants (pesticides, heavy metals and surfactants) seasonally, macrobenthos and bacteria (monthly) were done 2009 t0 2010 that located in Mangol Dam with longitude and latitude 52° 22' 49" N and 43° 15' 43" E, respectively. All water samples were collected from under-groundwater, surface water and wastewater in 12 stations and the results are as follow: The mean concentrations of DO, BOD5, COD, HCO3-, CO32-, TA, Ca2+, Mg2+, TH, TSS, TDS, Cl-, PO43-, NH4+, NO2- and NO3- were determined about 11.86±0.49, 5.95±0.11, 4.96±0.59, 28.1±6.1, 14.34±1.64, 36.30±2.33, 64.66±2.34, 28.84±1.52, 271.62±8.41, 0.37±0.03, 0.45±0.02, 28.02±1.96, 0.06±0.01, 0.010±0.001, 0.01±0.001 and 0.96±0.03 mg/l, respectively and also temperature, pH and EC were 12.7±0.61°C, 8.00±0.02 unit, 0.57±0.02 ms/cm in all samples. The surface water quality of Haraz River and its tributaries were suitable for usual applications such as aquatic matters, but based on BOD5 need to more attention for human usages. The water quality index (WQI) of surface water was classified in group II at all stations that shows serious water quality changes due to domestic and agriculture sewage. The groundwater was affected by different matters that caused change of water quality parameters. The wastewater was classified from good to moderate based on BOD5. The quality of wastewater was declined at down-stream with decreasing DO and pH and increasing BOD5 pollutants. The mean concentrations of aldrin and endrin were about 0.66 ± 0.59 and 0.71 ± 1.07 µg/l, respectively. The maximum seasonal concentration of organochlorin pesticides (OCPs) were detected in fall, winter and spring for endosulfan sulfate, γ-BHC, heptachlor, endrin aldehyde compounds about 2.85, 0.34, 0.14 and 0.14µg/l, respectively. The maximum seasonal percentage range of OCPs in fall, winter and spring for endosulfan sulfate, DDD and β-BHC were 42, 25 and 25 %, respectively. The maximum seasonal concentrations during fall, winter and spring were detected in Sorkhroud and Polechelave stations for only Fe about 1.48, 3.3 and 8.22 µg/l, respectively. Also for Hg in spring was determined about 17.2 mg/l. The mean concentration of Zn, Fe and Hg in water sampled were 0.23 ± 1.01 and 1.21 ± 2.00 µg/l, respectively and 4.65± 6.38 mg/l. The maximum concentration of linear alkyl benzene sulfonate (LAS) in summer was 971µg/l. The mean concentration of surfactant during fall, winter, spring and summer was 418.62 ± 52.16 µg/l. The maximum seasonal percentage ranges of surfactant during fall, winter, spring and summer in all stations of sampling were 76, 76, 100 and 100 %, respectively. Tottaly 15 families of macrobenthos were identified in Haraz River. The maximum mean of abundance and biomass in Sorkhroud and Nour-roud stations were 1177±256 ind/m2 and 3609 ± 935mg/m2, respectively. During Seasons, the maximum mean abundance and biomass were detected in fall and winter with 1185±444 ind/m2 and 3891±849 mg/m2, respectively. Water quality were classified by Hilsenhoff Biotic Index such as Sorkhrood station as "Very Poor", Isar town station as "Fairly Poor" and Karesang and Nor-roud stations as "good" qualification. The maximum of total coli forms and fecal coli forms in surface water were observed in Sorkhrood about 4.9 and 2.3CFU/100ml, respectively. The maximum of total coli forms and fecal coli forms in groundwater were observed in Halomsar and Absak stations about 4 and 1.7CFU/100ml, respectively. Maximum of total coli forms and fecal coli forms in wastewater were observed in Nor-roud station about 3.1 and 1.6CFU/100ml, respectively. The Ovary of nematode in wastewater were observed in Nor-roud and Isar Town stations about 30 through 124, respectively.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Physico-chemical characteristics ; Surface water ; Ground Water ; Wastewater ; Pesticides ; Heavy Metals ; Surfactants ; Macrobenthos ; Coliforms ; Nematodes ; Environmental pollutants ; Bacterial ; Temperature ; pH ; Biomass
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 225pp.
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  • 7
    Publication Date: 2021-05-19
    Description: The main purpose of this study was survey on achieving recombinant DNA vaccine (live delivery) against streptococcusis for Rainbow trout (Onchorhynchus mykiss) Immunization. Initially, a total of 515 samples were collected from the head kidney of diseased fish (weigh 50_ 200g) in 72 farms of 8 provinces. Approximately, 40% (206 samples) of specimens were infected to Streptococcus species. Then were isolated 172 DNA samples and consequently, five pathogenic species have been identified, including S. iniae, S. faecium, S. agalactiae, S. dysgalatiae, and S. uberis. The enzyme phosphoglucomutase (PGM) has recently been discovered to play an important role in polysaccharide capsule production and virulence in S. iniae. Therefore, was initially isolated S. iniae and cloning phosphoglucomutase gene. Then, the PGM gene was amplified successfully and cloned in pTZ57R cloning vector. The recombinant plasmid was sub cloned into pETD uet-l expression vector by restriction enzymes and confirmed by PCR. Meanwhile, for amplifying simA and cpsD genes were used universal primers pNZ8148 and special for simA and cpsD genes.The recombinant bacteria Lactococcus lactis (NZ9000) was used for transformation the plasmid into Lactococcus lactis. Vaccination was performed by oral, bath and injection (peritoneal) methods. The efficiency of g2 was better than g1in these three methods and all of groups. The determination of IgM level, or detection of anti S. iniae antibody was carried out by using ELISA. The results revealed that there was a significant (p〈0.05) difference between the level of IgM in all three methods and experiment groups compare to control group. The results of challenge of vaccinated fish with S. iniae showed that fish RPS in all of groups except group 3 (42.63 percent) were more than 50 percent while, in control group was 21.43 percent. The highest fish RPS was belong to group 11(61.25 percent) and statistical analyses revealed that significant (p〈0.05) difference between fish vaccinated RPS, compare to control group.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Survey ; DNA ; Live delivery ; Rainbow trout ; Onchorhynchus mykiss ; Immunization
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 82pp.
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  • 8
    Publication Date: 2021-05-19
    Description: Due to various Physic and chemical factors of rivers leading to the sea, the situation seems different topography and throughput rate initial productions in the different seasons and due to various circumstances physical and chemical rivers leading to the sea, seabed topography in different situation appears to be of primary production in the eastern and western between the Caspian Sea in the season, may be altered.Identifying species and determining the distribution and biomass of the changes and how they are affected by environmental changes and we are environmentally conscious. We also compare the current situation with previous studies, we find that the number and types of plankton biomass have been what it is. During 1392 in spring, summer, autumn and winter, in a study of 8 transects of 40 stations. In each transect from Astara to the Turkmen. 5 stations at depths of 5. 10 and 20 m were selected for sampling. The total number of 182 species from seven branches Bacillariophyta, Pyrrophyta, Cyanophyta, Chlorophyta, Euglenophyta, Xantophyta and Chrysophyta phytoplankton were identified. Including 81 species of Bacillariophyta, 33 Cyanophyta, 25 Pyrrophyta, 31Chlorophyta , 9 Euglenophyta, 1 Xantophyta and Chrysophyta had 1 specie. Most of Bacillariophyta (61 species) was in Autumn and then in winter (48 species). Dominant species of Bacillariophyta were Pseudonitzschia seriata, Rhizosolenia fragilissima, Stephanodiscos sp. , Melosira varians, Nitzschia acicularis and Cyclotella menenghiniana Pyrrophyta was greatest diversity of branches in summer, autumn and winter (19 species), which includes Exuviaella cordata, Exuviaella marina, Prorocentrum praximum and Prorocentrum scutllum. In the autumn density of Cyanophyta was 285/7(± 137/1) cubic meters × 106 and biomass was 95( ± 54) mg per cubic meter) and 18 species were observed. The dominant species in this category were Oscillatoria sp., Nodularia spumigena and Oscillatoria agardhii. Most species of Chlorophyta branche in autumn and winter and summer median region with the highest density at the density of 26/2% and most of it is Binuclearia lauterbornii. Identified as the branches Euglenophyta were Trachelomonas, Euglena and Phacus that were observed in all seasons. In winter, the highest mean biomass was 9( ± 0/818) mg per cubic meter and the highest density of in summer was 0/5 ( ± 0/5) in cubic meters × 106. In winter the depth of 10 meters and surface of Babolsar, Amir Abad and Anzali, a kind of Chrysophyta and in surface of Tonekabon and Anzali a species of Xantophyta were observed that had negligible density and biomass. Studies have shown that density and biomass of Bacillariophyta were 228 (± 471) per cubic meter × 106) and 6157 ± ( 290) mg per cubic meter) respectivity and Pyrrophyta were 28/17( ± 27/14) cubic meter × 106in cubic meters) and 3349 ( ± 336) mg per cubic meter) and Cyanophyta 120/40 (± 123/87) per cubic meter × 106 per cubic meter), biomass (55 ± 57mg per cubic meter) were the branches of the dominant phytoplankton. Abundance and biomass in different seasons have been significant differences (p 〈0.05).
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Chemical ; Physical ; Environmental ; Photosynthetic product ; Phytoplankton ; Species ; Biomass ; Plankton ; Sampling ; Bacillariophyta ; Pyrrophyta ; Cyanophyta ; Chlorophyta ; Euglenophyta ; Xantophyta ; Abundance
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 87pp.
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  • 9
    Publication Date: 2021-05-19
    Description: Streptococcosis is an acute infectious disease that causes mortality in marine and freshwater aquacultures. One of the most important hosts is susceptible to the disease of rainbow trout and during the past decade its industrial production has been growing in Iran. According to FAO, Iran is among the 10 countries producing fish in the world. The most important bacteria causing Strptococcosis includes Strptococcus, Vagococcus and Enterococcus genera. But in all cases, the clinical sings are the same. More than a decade has passed since the first report of this disease in Iran and unfortunately, this disease has become the most important problems of trout production in Iran. Therefor, 72 farms were selected in 8 provinces including Mazandaran, Gilan, Lorestan, CharmahalBakhtiyari, Fars, Kermanshah, Tehran and Kokiloye-Boyer Ahmad and a total 520 moribund and apparently healthy rainbow trout (weight 50 – 200gr) were collected during summer 2008 to 2009. Fish kidney, spleen and liver samples were culture aseptically and finally 206 isolates were identified as gram positive cocci. Using conventional biochemical test, S.uberis, S.agalactiae, S.dysgalactiae, S.faecium and S.inia. Additionally, 172 from 206 isolates were confirmed as S.uberis ، S.agalactiae ، S.dysgalactiae ، S.faecium and S.inia using a PCR assay. Based on the results, the most prevalent is belong to S.uberis (isolated from 5 of 7 province), S.dysgalactiae (isolated from 4 of 7 province), S.agalactiae (isolated from 2 of 7 province) and S.faecium and S.inia (from 1 of 7 province) respectively. It is necessary to mention, S.inia was identified just to Fars and any gram positive cocci was isolated from Tehran.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Molecular ; Streptococcosis ; Rainbow trout ; Oncorhynchus mykiss ; Walbaum ; Disease ; Mortality ; Marine ; Freshwater ; Aquaculture ; S.uberis ; S.agalactiae ; S.dysgalactiae ; S.faecium ; S.inia
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 43pp.
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  • 10
    Publication Date: 2021-05-19
    Description: This paper described hybrid between two species of Salmonidae Rainbow trout (Oncorhynchus mykiss) & Caspian salmon (Salmo trutta caspius). Caspian salmon is a native fish in the Caspian Sea which migrate for spawning to the Mazandaran Rivers in the northern part of Iran during the autumn. Annually some broodstock of this type of fish are caught from the rivers and carried to fish farm in order to produce smolts. Then released them to the rivers to preserve stock Rainbow trout is also used for intensive culture in Iran since we would like to have inherit intermediate of these species. After selection of broodstock and fertilization we assayed many factors such as: Feeding, growth, systematic, cytogenetic, internal organs, tolerance to deficiency of oxygen and high temperature. In addition, we also compared with control. The result showed that the hybrid is intermediate between parental, and suitable for intensive culture.
    Description: Published
    Keywords: Species ; Salmonidae ; Rainbow trout ; Oncorhynchus mykiss ; Caspian salmon ; Salmo trutta caspius ; Smolt ; Spawning ; Migrate ; Broodstock ; Fertilization ; Feeding ; Growth ; Systematic ; Cytogenetic ; Hybridization
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.46-54
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