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  • Immunocytochemistry  (70)
  • Drosophila  (47)
  • 42.80  (46)
  • Coleoptera  (44)
  • Springer  (207)
  • Annual Reviews
  • Blackwell Publishing Ltd
  • Massachusetts Institute of Technology and Woods Hole Oceanographic Institution
  • 2020-2024
  • 2005-2009
  • 1990-1994
  • 1980-1984  (207)
  • 2009
  • 1984  (102)
  • 1982  (105)
Collection
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  • 2020-2024
  • 2005-2009
  • 1990-1994
  • 1980-1984  (207)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Pitfall-trap catches of the carabid beetle Pterostichus melanarius, in relation to gut contents and prey densities, in insecticide treated and untreated spring barley (1984)
    Springer
    Entomologia experimentalis et applicata 36 (1984), S. 23-30 
    Details
    ISSN: 1570-7458
    Keywords: Pterostichus melanarius ; Coleoptera ; Carabidae ; pitfall traps ; insecticides ; prey ; hunger ; activity ; Rhopalosiphum padi ; Fenitrothion ; Sumicidin (Fenvalerate) ; spring barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Au cours d'essais à grande échelle, effectués en 1981 et 1982, sur orge de printemps, pour évaleur l'effet des insecticides Fenitrothion et Sumicidine (fenvalerate) sur les arthropodes utiles, un accroissement significatif des captures, dans des trappes pièges de Barber, de Pterostichus melanarius (Illiger) a été observé plusierus semaines après le traitement dans les lots traités par rapport aux lots témoins. Significativement plus de femelles de P. melanarius ont été capturées dans les parcelles traitées pendant cette période d'accroissement après traitement. Une diminution correspondante dans les populations de proies a été observée à la suite des traitements. Chaque femelle de P. melanarius des parcelles traitées avait une fraction significativement moins importance de son intestin remplie d'aliments solides (arthropodes) que celles des parcelles non traitées. Des auteurs précédents avaient observé des taux de capture plus rapides dans les trappes et ne plus grande mobilité des carabes affamés. La réduction des populations proies par l'application d'insecticides affamerait les carabes dont l'activité serait par suite plus élevée. Comme le taux de captures dans les trappes est dû à la fois à la taille de la population et à son activité, on en déduit que la capture par trappes seule ne peut donner une image exacte de l'effet des insecticides sur les populations de carabes dans les champs.
    Notes: Abstract During large-scale field experiments in 1981 and 1982, designed to assess the effects of the insecticides Fenitrothion and Sumicidin (Fenvalerate) on beneficial arthropods in spring barley, significant increases in pitfall-trap catches of Pterostichus melanarius (Illiger) were observed in treated plots compared to untreated controls, several weeks after treatment. Significantly more female P. melanarius were caught in treated plots during these post-treatment increases. Corresponding decreases were observed in prey populations following treatments. Individual female P. melanarius from treated plots had significantly fewer of their gut areas full of solid (arthropod) food when compared to those from untreated control plots. Earlier workers observed both faster recapture rates in pitfalls and higher mobility in hungry carabid beetles. The following hypothesis is suggested: reducing prey populations by the application of insecticides results in hungrier carabid beetles with consequently higher activity. Since pitfall-trap catch is determined not only by population size but also activity, it is argued that pitfall-trap catches alone cannot give a true measure of the effects of insecticides on carabid populations in the field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00345200
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  • 2
    Electronic Resource
    Electronic Resource
    Distribution of proteinases and carbohydrases in the midgut of larvae of the sweetpotato weevilCylas formicarius elegantulus and response of proteinases to inhibitors from sweet potato (1984)
    Springer
    Entomologia experimentalis et applicata 36 (1984), S. 97-105 
    Details
    ISSN: 1570-7458
    Keywords: Coleoptera ; Curculionidae ; Cylas formicarius elegantulus ; sweetpotato weevil ; sweet potato ; digestion ; enzyme distribution ; trypsin-inhibitors ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé L'activité endoprotéinase était limitée au liquide de la lumière des régions ventriculaires antérieure et postérieure de l'intestin moyen des larves deCylas formicarius elegantulus Summers. Les aminopeptidases ont été trouvées dans le liquide de la lumière (18%), mais étaient principalement associées avec les fractions insolubles des cellules du ventricule postérieur (82%). Suivant le substrat, l'activité carboxypeptidase était à peu près également répartie entre le liquide de la lumière et les fractions insolubles des cellules du ventricule postérieur. L'amylase, enzyme secrétée, a été observée à la fois dans la lumière antérieure et postérieure. Cinq bandes d'activité amylase ont été révélées sur zymogrammes d'amidon après électrophorèse d'intestins moyens entiers. Trois des bandes d'amylase pouvaient provenir de la plante. Il y avait au moins 2 α-glucosidases, une secrétée et une liée aux cellules du ventricule antérieur. L'activite β-glucosidase était liée aux cellules du ventricule antérieur, fandis que l'activité α-galactosidase était limitée au fluide de la lumière. Les activités β-galactosidase et cellulase ont été trouvées à la fois dans le liquide de la lumière et dans la fraction cellulaire insoluble. Ainsi, la digestion initiale des grosses protéines et des polymères de carbohydrates se produit dans le liquide de la lumière dans les régions ventriculaires antérieure et postérieure. Cependant, l'achèvement de la digestion des oligopeptides se produit principalement dans le ventricule postérieur, tandis que la fin de la digestion des oligosaccharides se produit dans le ventricule antérieur. Les concentrations en inhibiteurs de trypsine de 5 cultivars d'I. batatas L. diffèrent de 1 à 20. Les endoprotéinases deC. formicarius elegantulus ont été inhibées par des extraits de cultivars, mais les cultivars avec des concentrations relativement élevées d'inhibiteur avainent antérieurement montré être susceptibles d'être attaqués par le coléoptère dans des essais en champs.
    Notes: Abstract Endoproteinase activity was confined to luminal fluid from anterior and posterior ventricular regions of midguts of larvae of the sweetpotato weevil,Cylas formicarius elegantulus (Summers). Aminopeptidase was found in luminal fluid (18%) but was primarily associated with insoluble fractions from cells of the posterior ventriculus (82%). Depending on substrate, carboxypeptidase activity was about equally distributed between luminal fluid and insoluble fractions from posterior ventriculus cells. Amylase was found in luminal fluid in both the anterior and posterior ventriculus. Five bands of amylase activity were detected on starch zymograms following electrophoresis of whole midgut samples. Three of the amylase bands may be plant-derived. At least two α-glucosidases were present, one secreted and one bound to anterior ventriculus cells. β-Glucosidase activity was bound to anterior ventriculus cells whereas α-galactosidase activity was confined to luminal fluid. β-Galactosidase and cellulase activities were found in both luminal fluid and the insoluble cell fraction. Thus, initial digestion of large protein and carbohydrate polymers occurs in luminal fluid in both anterior and posterior ventriculus regions. However, terminal digestion of oligopeptides occurs primarily in the posterior ventriculus while terminal digestion of oligosaccharides occurs in the anterior ventriculus. A 20-fold difference in trypsin-inhibitor concentration was found among five sweetpotato cultivars. Endoproteinases from sweetpotato weevil larvae were inhibited by extracts from the cultivars but cultivars with relatively high concentrations of inhibitor were previously shown to be susceptible to weevil attack in field trials.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00569949
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  • 3
    Electronic Resource
    Electronic Resource
    Characteristics of the S/N ratio of the beat note obtained from the frequency-mixing experiments between a CO2 and an H2O laser (1984)
    Springer
    Applied physics 34 (1984), S. 49-53 
    Details
    ISSN: 1432-0649
    Keywords: 62.65 ; 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The S/N ratio of the beat note obtained from the frequency mixing between a 32 THz CO2 laser, a 10.7 THz H2O laser and a 22 GHz klystron is measured as a function of incident power of both lasers and the klystron on the W-Ni point contact diode. A maximum S/N ratio of 36 dB is obtained. It is found that the S/N ratio arises from the product of the contributions due to the incident power and the fifth coefficient of the current-voltage characteristic of the W-Ni diode. These characteristics are discussed qualitatively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00697947
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  • 4
    Electronic Resource
    Electronic Resource
    Ninth-order nonlinear polarization and vuv generation in Hg vapor (1984)
    Springer
    Applied physics 34 (1984), S. 171-173 
    Details
    ISSN: 1432-0649
    Keywords: 42.65 ; 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Interference of resonant ninth-order and nonresonant seventh-order nonlinear polarizations in Hg atoms has been studied, and direct conversion of fundamental ir radiation into the vuv has been obtained. The interference has been observed in the form of a Fano-Boitler contour. The efficiency of generation by ninth-order processes appears to be higher than that by seventh-order.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00697511
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  • 5
    Electronic Resource
    Electronic Resource
    Optical-gyroscope application of efficient crossed-channel acoustooptic devices (1984)
    Springer
    Applied physics 35 (1984), S. 113-118 
    Details
    ISSN: 1432-0649
    Keywords: 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract An acoustooptic (AO) deflector/modulator using a single-mode crossed-channel waveguide of 2Δn type and an interdigital transducer is fabricated in aY-Z LiNbO3 substrate. This module has shown a high diffraction efficiency. A 50% diffraction efficiency and a bandwidth of 13.4 MHz were obtained with 0.13 W of surface-acoustic wave power centered at 320MHz. Since the cross section of the channel waveguide and that of the optical fiber are comparable, the interfacting of the resultant acoustooptic devices with fiber optical systems would greatly simplified. In addition, the frequency-shifted optical beam can be conveniently used as a reference signal or local oscillator in heterodyne detection. Consequently, this cross-channel acoustooptic device should find a variety of applications in realizing an integrated-optics module with a 50–50% power slit, optical communication, and an optical fiber system. One of those applications, optical gyroscopes, is proposed by using this kind of crossed-channel AO device.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00697430
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  • 6
    Electronic Resource
    Electronic Resource
    Novel spectroscopy (1982)
    Springer
    Applied physics 28 (1982), S. 297-305 
    Details
    ISSN: 1432-0649
    Keywords: 32 ; 42.80 ; 33 ; 82 ; 34
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00697859
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  • 7
    Electronic Resource
    Electronic Resource
    Origin of laser-output noise of cataphoretic HeSe+ lasers (1982)
    Springer
    Applied physics 28 (1982), S. 373-381 
    Details
    ISSN: 1432-0649
    Keywords: 42.55 ; 42.80 ; 52
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We investigated noise and fluctuations of the output power of cataphoretic HeSe+ lasers in positive column plasma. Direct coupling of laser output power noise and fluctuations of the local population inversion was found. An investigation of the positive column plasma showed moving striations being responsible for the gain fluctuations. Whereas the local plasma properties are dominated by high frequency striations in the 100 kHz range, integrated quantities such as laser gain per pass are most strongly influenced by low-frequency waves with a continuous noise spectrum below 200 kHz. External modulation of the discharge voltage or current at a frequency near thehf striations reduces laser noise and increases laser output power.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00686369
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  • 8
    Electronic Resource
    Electronic Resource
    Bragg diffraction of gaussian beams by thick gratings: Numerical evaluations by plane-wave decomposition (1982)
    Springer
    Applied physics 28 (1982), S. 383-390 
    Details
    ISSN: 1432-0649
    Keywords: 42.10 ; 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The validity of 2-D coupled-wave theory is investigated for the case of Bragg diffraction of a Gaussian beam by a thick unslanted phase grating in transmission mode. Fourier plane wave decomposition theory, as presented in a companion article, is used to test 2-D coupled-wave theory under circumstances when its validity is in question, namely when the incident distribution is very narrow or fast-varying, or when the parameterv o, due to Kogelnik (1969), which is proportional to the product of coupling rate and grating thickness, is very large. Numerical evaluations of the field patterns at output from the grating, obtained using plane-wave decomposition, are used to explore the effect of the gradual violation of the conditions (from the previous article) under which 2-D coupled-wave theory is valid.
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    URL: http://dx.doi.org/10.1007/BF00686370
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  • 9
    Electronic Resource
    Electronic Resource
    Temporal analysis of a mode-locked Nd-glass laser by four-wave mixing (1982)
    Springer
    Applied physics 29 (1982), S. 263-267 
    Details
    ISSN: 1432-0649
    Keywords: 42.55 ; 42.65 ; 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The duration, shape, and background intensity level of pulses of a passively modelocked Nd-glass laser are determined by noncollinear phase-matched four-photon frequency mixing of time-separated fundamental and second-harmonic pulses in a KI crystal. The pulse shape is found to be slightly asymmetric with slower trailing than rising parts. The background intensity level before and behind the main pulse is about 5¢10−6 the peak pulse intensity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00689185
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  • 10
    Electronic Resource
    Electronic Resource
    A laser optical method for the determination of tropospheric OH concentrations (1984)
    Springer
    Applied physics 33 (1984), S. 103-113 
    Details
    ISSN: 1432-0649
    Keywords: 42.68 ; 42.80 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A long-path absorption procedure for the determination of tropospheric OH concentrations is described in detail. Initial measurements using this method were carried out in Frankfurt a. M. By including in the evaluation the visible radiation of a frequencydoubled dye laser used in the measurements, it was possible to mathematically reduce the signal fluctuations caused by the scattering and density fluctuations of tropospheric air. The resulting detection limit was 3×106 OH per cm3. SO2 concentrations which had to be simultaneously determined in order to eliminate an interference effect, could be detected in the range of 1–40 ppb on the absorption path.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688355
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  • 11
    Electronic Resource
    Electronic Resource
    Guided waves and IR lasers (1982)
    Springer
    Applied physics 28 (1982), S. 283-289 
    Details
    ISSN: 1432-0649
    Keywords: 85.60 ; 42.80 ; 42.55 ; 33
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00697857
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  • 12
    Electronic Resource
    Electronic Resource
    Light-polarization modifications in a multipass cavity (1982)
    Springer
    Applied physics 29 (1982), S. 43-54 
    Details
    ISSN: 1432-0649
    Keywords: 42.80 ; 32
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract This paper analyses the slight change undergone by the polarization of a laser beam performing a multipass between two spherical mirrors facing each other. We replace the general 16-parameter Mueller formalism by an approximate 7-parameter one, and we give a quantitative experimental criterion for the validity of this approximation. Then we apply this formalism to the analysis of our experimental data. The results evidence:i) intrinsic birefringence in the mirror coatings (typical value per reflexion ∼2 to 4×10−4 rad);ii) birefringence due to off-normal incidence (typically 3.6×10−5 rad per reflexion for an incidence of 2×10−2 rad);iii) optical rotation, of purely geometrical origin (typically 14° for a multipass with revolution symmetry and 100 forward-backward passes under constant incidencei=2°);iv) no observable circular dichroism (≲10−6). Finally these results are discussed in connection with possible associated systematics in a parity violation experiment which motivated this work.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00694368
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  • 13
    Electronic Resource
    Electronic Resource
    Coherent transient phenomena (1982)
    Springer
    Applied physics 29 (1982), S. 179-182 
    Details
    ISSN: 1432-0649
    Keywords: 33 ; 34 ; 36 ; 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688662
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  • 14
    Electronic Resource
    Electronic Resource
    Simultaneous phase-lock of five CO2 lasers to a primary Cs frequency standard (1984)
    Springer
    Applied physics 35 (1984), S. 119-122 
    Details
    ISSN: 1432-0649
    Keywords: 42.72 ; 42.80 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The output of a CO2 laser, operating on theP I(18) transition of13C16O2 at 26941 GHz (11.128 μm) was phase-locked to a 5 MHz signal from a primary Cs frequency standard by means of a frequency chain having only CO2 lasers as infrared sources. Simultaneously, four other CO2 lasers in the chain were phase-locked to the 26941 GHz output. This provided CO2 laser frequencies at 26 450 305, 26 940 815, 28 694 625, 29 442 480, and 33 185 715 MHz having zero long-term-average frequency error relative to the Cs standard, and the ±10−13 (≃3 Hz) long-term absolute uncertainty of the standard.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00697698
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  • 15
    Electronic Resource
    Electronic Resource
    Ellipsometry on magnetooptic thin film multilayer systems (1984)
    Springer
    Applied physics 34 (1984), S. 139-143 
    Details
    ISSN: 1432-0649
    Keywords: 42.80 ; 78.20L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The optical and magnetooptical properties of thin film multilayer systems including a ferromagnetic absorbing layer were measured by ellipsometry. These were compared to calculations, using the ellipsometric data of each individual layer and measuring their thicknesses by FECO to determine the refractive indices and magnetooptical parameters of each layer for the calculation of the whole stack. Agreement between calculated and measured properties of the stack showed to be strongly dependent on the precision in measuring film thickness.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00697506
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  • 16
    Electronic Resource
    Electronic Resource
    Sub-Doppler excited molecule energy transfer spectroscopy (1982)
    Springer
    Applied physics 27 (1982), S. 153-156 
    Details
    ISSN: 1432-0649
    Keywords: 33 ; 42.80 ; 07.65
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A sub-Doppler spectroscopic technique involving the detection of vibrational energy transfer in excited molecules with selected velocity components at a microphone diaphragm is demonstrated. The technique is applied to the investigation of thev 3 R(16) transition in HCN and the results compared with a previously derived theoretical model.
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    URL: http://dx.doi.org/10.1007/BF00694641
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  • 17
    Electronic Resource
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    F-center-laser. Superconductivity (1982)
    Springer
    Applied physics 28 (1982), S. 306-310 
    Details
    ISSN: 1432-0649
    Keywords: 68 ; 42.70 ; 42.60 ; 61.70 ; 61.50 ; 42.80 ; 29
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00697860
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  • 18
    Electronic Resource
    Electronic Resource
    Formation of blazed holographic gratings (1982)
    Springer
    Applied physics 28 (1982), S. 349-353 
    Details
    ISSN: 1432-0649
    Keywords: 42.80 ; 42.82
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Various methods for the recording of blazed holographic diffraction gratings are presented and the regions in the wavelength-groove density plane for which they can be used are shown. The gratings are made in the positive photoresist Shipley AZ-1350. The diffraction efficiency for s and p polarization was measured as a function of wavelength and angle of incidence. An absolute efficiency of 80% was achieved.
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    URL: http://dx.doi.org/10.1007/BF00686365
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  • 19
    Electronic Resource
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    Conditions for the harmonic-like and efficient amplitude modulation of the cw gaussian laser beam by means of a mechanical chopper (1984)
    Springer
    Applied physics 34 (1984), S. 1-4 
    Details
    ISSN: 1432-0649
    Keywords: 42.80 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The values of the chopping disc slot and mark space widths relative to the radius of the Gaussian beam are found, which achieve optimum harmonic-like and efficient amplitude modulation of the cw laser beam. The simple approximation for the waveform of the modulated laser power valid for these optimum or near optimum values of the chopping disc parameters is presented.
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    URL: http://dx.doi.org/10.1007/BF00697456
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  • 20
    Electronic Resource
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    Apparent transfer function for partially coherent optical information processing (1982)
    Springer
    Applied physics 28 (1982), S. 359-366 
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    ISSN: 1432-0649
    Keywords: 42.30 ; 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract In this paper, the general formulations of the apparent transfer function for the partially coherent optical processor will be derived. Although these formulas show that the apparent transfer function is dependent upon the degree of spatial and temporal coherence, there is actually more variability in the spatial coherence. We note that the obtained formulas may also be used as a criterion in the selection of source size and spectral bandwidth of an incoherent light source. Thus a specific optical information processing operation can be carried out with an incoherent source.
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    URL: http://dx.doi.org/10.1007/BF00686367
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  • 21
    Electronic Resource
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    Development of an optical time scale (1982)
    Springer
    Applied physics 29 (1982), S. 63-65 
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    ISSN: 1432-0649
    Keywords: 42.60 ; 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A time standard based on the use of an optical oscillation period of a frequencystable He−Ne laser as a time scale is first described. We obtained highly frequency-stable oscillations in the SHF range that were locked to the oscillations of a He−Ne laser stabilized to an absorption resonance in methane at 3.39 μm. A direct comparison of frequency stabilities of a rubidium standard and He−Ne/CH4 laser has been made. The absolute measurement of the frequency of the He−Ne/CH4 laser we performed gave a new value of frequency.
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    URL: http://dx.doi.org/10.1007/BF00694370
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  • 22
    Electronic Resource
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    Energy transfer processes in two different bifluorophoric laser dyes (1982)
    Springer
    Applied physics 29 (1982), S. 73-77 
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    ISSN: 1432-0649
    Keywords: 82 ; 78.60 ; 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The mechanism of intramolecular energy transfer in two bifluorophoric laser dyes has been investigated by measurements of laser parameters, excited state absorption and phosphorescence spectra. The new value found for the dimethyl-POPOP triplet energy (48.0 kcal/mole) accounts for the different behaviour of the two classes of bifluorophoric dyes used in this investigation.
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    URL: http://dx.doi.org/10.1007/BF00692766
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  • 23
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    Novel quantum-electronics phenomena and devices (1982)
    Springer
    Applied physics 29 (1982), S. 152-156 
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    ISSN: 1432-0649
    Keywords: 42.80 ; 42.55
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688656
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  • 24
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    Dynamical nonlinear optics and bistability (1982)
    Springer
    Applied physics 29 (1982), S. 169-175 
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    ISSN: 1432-0649
    Keywords: 42.80 ; 42.65
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688660
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  • 25
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    Ethylene monitoring by differential absorption with a CO2 waveguide laser (1982)
    Springer
    Applied physics 29 (1982), S. 227-233 
    Details
    ISSN: 1432-0649
    Keywords: 86 ; 70L ; 07.60 ; 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract In this paper we report on a differential absorption technique using a CO2 waveguide laser. The method is based on the measurement of the differential absorption between two different frequencies of the same selected CO2 laser line. The sensitivity of the technique depends strongly on the total pressure and has been estimated to be about a few tens of ppb or better over a 1 km path length in C2H4 diluted with air to a total pressure of a few tens of torrs. Its relative simplicity and high sensitivity at low total pressure enable this technique to be applied to gas detection either at high altitude or to local monitoring of a low pressure sample.
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    URL: http://dx.doi.org/10.1007/BF00688673
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    Harmonic detection with tunable diode lasers —two-tone modulation (1982)
    Springer
    Applied physics 29 (1982), S. 279-285 
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    ISSN: 1432-0649
    Keywords: 07.65 ; 42.80
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    Topics: Physics
    Notes: Abstract The minimum absorption detectable by a tunable diode laser spectrometer utilising harmonic techniques is often limited by interference fringes generated by scattered light. The sensitivity of the spectrometer to absorption can be increased by applying a jitter modulation. In this paper, the theory of harmonic response for single- and two-tone modulation over optical fringes and Lorentzian absorption lines is developed and compared to experimental measurements. A simple analytic expression for the two-tone harmonic line shape is derived. This expression provides a physical understanding of the effects of the second modulation, and a means to unravel the effects of the second modulation on the linewidth and line shape. For a specific choice of the jitter frequency and phase, it is possible to simultaneously minimise the fringe signal and increase the harmonic absorption signal. The results of this investigation are applicable to trace gas detection using tunable diode lasers, and to other areas of spectroscopy and magnetic resonance where harmonic techniques are used.
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    URL: http://dx.doi.org/10.1007/BF00689188
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    Effects of a lossy thin plasma-film on metal-diffused LiNbO3 optical waveguides (1984)
    Springer
    Applied physics 35 (1984), S. 23-30 
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    ISSN: 1432-0649
    Keywords: 42.80 ; 41
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    Topics: Physics
    Notes: Abstract I intend to investigate the effects of different kinds of partially reflecting metal film (plasma layer) on the wave characteristics of a stratified titanium-diffused LiNbO3 optical waveguide. The mode number, the equivalent indices, the extinction coefficients, the field distributions and the powers carried in each layer are given as a function of the free-space wavelength, of the diffusion parameters, and of the opticc-axis orientation. Of course, the power of TM-like hybrid waves flows in opposite directions in the plasma and the other regions. For ac-axis varying in the transverse plane, the ith-order hybrid mode is rather complicated in the guide with an aluminum layer. There, in fact, the field mapping can be seen as an overlap of a TE i with a TM i+1, mode because of a sudden transformation of the TM0 mode into a superficial plasma wave (SPW). On the contrary, with a silver layer, the hybrid field is a simple combination of a TE i with a TM i mode with no SPW growing. The guide losses assume the lowest values (1 dB/cm) for an Ag layer which is the prime candidate for making electro-optical or acoustic-optical devices. On the other hand, the nickel film causes the highest losses (≧66 dB/cm).
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    URL: http://dx.doi.org/10.1007/BF00697517
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    A diffraction grating autocorrelator for measurement of single ultrashort light pulses (1984)
    Springer
    Applied physics 35 (1984), S. 45-48 
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    ISSN: 1432-0649
    Keywords: 42.80 ; 06.60Jn
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    Notes: Abstract A simple autocorrelation scheme for measurement of single ultrashort light pulses in the 1–500 ps range is proposed. Only a diffraction grating and a mirror are utilized to obtain a variable time delay between two collinear beams. The advantages of the proposed method and the effect of the grating dispersion on the autocorrelator performance are discussed.
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    URL: http://dx.doi.org/10.1007/BF00697520
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    Low threshold optical bistability with optical pumping (1982)
    Springer
    Applied physics 29 (1982), S. 79-87 
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    ISSN: 1432-0649
    Keywords: 42.50 ; 42.65 ; 42.80
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    Topics: Physics
    Notes: Abstract By use of circularly polarized light we have exploited optical pumping within the Zeeman sublevels of the ground state to obtain optical bistability in sodium vapours with low threshold power (around 1 mW) and a wide tuning range (〉12 GHz). Experimental results are presented for different conditions of operation and compared to computer calculations, that include propagation effects and inhomogeneous broadening, based on a three-level atomic model that accounts for optical pumping.
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    URL: http://dx.doi.org/10.1007/BF00692767
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    The analysis of excite and probe measurements of relaxation times with fluctuating pulse duration (1982)
    Springer
    Applied physics 29 (1982), S. 135-138 
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    ISSN: 1432-0649
    Keywords: 06 ; 42.80
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    Topics: Physics
    Notes: Abstract The effect of fluctuations in the pulse duration of synchronously pumped modelocked pulse trains on excite and probe measurements is discussed. Relaxation times comparable with the pulse durations can be measured even when large pulse-to-pulse fluctuations in duration exist. The pump and probe pulse durations are assumed to be correlated. When the probe pulses are the second harmonic of the pump, or vice versa, the third harmonic must also be generated to permit deconvolution of experimental excite and probe data. When the pump and probe pulses have the same time dependence, the excite and probe curves consist of the desired response function convolved with the time-averaged second harmonic autocorrelation function which is easily measured. Deconvolution yields the relaxation time but fluctuations in pulse duration increase the root-mean-square voltage fluctuation at the output of the detector system and limit the accuracy with which the relaxation time can be calculated.
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    Surface spectroscopy (1982)
    Springer
    Applied physics 29 (1982), S. 182-185 
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    ISSN: 1432-0649
    Keywords: 36 ; 42.80 ; 52
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    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688663
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    Anisotropic selfdiffraction in BaTiO3 (1984)
    Springer
    Applied physics 35 (1984), S. 17-21 
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    ISSN: 1432-0649
    Keywords: 42.70 ; 42.65 ; 42.80
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    Topics: Physics
    Notes: Abstract Anisotropic selfdiffraction in BaTiO3 crystals utilizing the large electrooptic coefficientr 42 is described. In the absence of an external electric field the generated space charge field is determined by diffusion processes. The theory is in agreement with the experimental results. The angles between extraordinary and ordinary beams, the intensity and time dependence of transmitted and diffracted beams, and phase conjugation effects are measured and evaluated.
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    Three-dimensional surface measurement using the confocal scanning microscope (1982)
    Springer
    Applied physics 27 (1982), S. 211-213 
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    Keywords: 06 ; 42.80
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    Topics: Physics
    Notes: Abstract The use of the depth discriminination property of the confocal scanning microscope for surface profiling has been adapted to provide a method of high-resolution three-dimensional surface profilometry. Measurements on a semiconductor specimen demonstrate the technique; depth variations of the order of 0.1 μm are clearly resolved.
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    On the relation between genetic and environmental variability in animals (1982)
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    Journal of molecular evolution 18 (1982), S. 310-314 
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    ISSN: 1432-1432
    Keywords: Neutral mutation theory ; Natural selection ; Protein evolution ; Levene model ; Environmental variability ; Genetic variability ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary If a phenotypic character is under stabilizing selection, the selective disadvantage of a nonoptimal genotype will decrease exponentially to zero as the proportion of phenotypic variation that is environmental in origin -V e /V p - increases. Under the modified mutation-drift hypothesis of genetic polymorphism, the proportion of mutations that are effectively neutral and average heterozygosity should increase with this ratio. Invertebrates, because of their small size, fast development, and low degree of homeostasis (relative to vertebrates), are expected to show a larger environmental component of phenotypic variation than vertebrates. This may help explain why invertebrates are in general more genetically variable than vertebrates and why, when laboratory populations ofDrosophila are maintained in heterogeneous environments, genetic variability is lost less rapidly than when they are kept in constant conditions.
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    Adjacent chromosomal regions can evolve at very different rates: Evolution of theDrosophila 68C glue gene cluster (1984)
    Springer
    Journal of molecular evolution 20 (1984), S. 251-264 
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    ISSN: 1432-1432
    Keywords: Drosophila ; Genome evolution ; 68C Glue gene cluster ; Drosophila melanogaster species subgroup
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The 68C puff is a highly transcribed region of theDrosophila melanogaster salivary gland polytene chromosomes. Three different classes of messenger RNA originate in a 5000-bp region in the puff; each class is translated to one of the salivary gland glue proteins sgs-3, sgs-7, or sgs-8. These messenger RNA classes are coordinately controlled, with each RNA appearing in the third larval instar and disappearing at the time of puparium formation. Their disappearance is initiated by the action of the steroid hormone ecdysterone. In the work reported here, we studied evolution of this hormone-regulated gene cluster in themelanogaster species subgroup ofDrosophila. Genome blot hybridization experiments showed that five other species of this subgroup have DNA sequences that hybridize toD. melanogaster 68C sequences, and that these sequences are divided into a highly conserved region, which does not contain the glue genes, and an extraordinarily diverged region, which does. Molecular cloning of this DNA fromD. simulans, D. erecta, D. yakuba, andD. teissieri confirmed the division of the region into a slowly and a rapidly evolving protion, and also showed that the rapidly evolving region of each species codes for third instar larval salivary gland RNAs homologous to theD. melanogaster glue mRNAs. The highly conserved region is at least 13,000 bp long, and is not known to code for any RNAs.
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    Source encoding for partially coherent optical processing (1982)
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    Applied physics 27 (1982), S. 99-104 
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    ISSN: 1432-0649
    Keywords: 42.30 ; 42.80
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    Topics: Physics
    Notes: Abstract A relation between spatial coherence function and source encoding intensity transmittance function is presented. Since the spatial coherence is depending upon the information processing operation, a strictly broad spatial coherence function may not be required for the processing. The advantage of the source encoding is to relax the constraints of strict coherence requirement, so that the processing operation can be carried out with an extended incoherent source. Emphasis of the source encodings and experimental demonstrations are given. The constraint of temperal coherence requirement is also discussed.
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    Bragg diffraction of finite beams by thick gratings: Two rival theories (1982)
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    Applied physics 28 (1982), S. 63-72 
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    ISSN: 1432-0649
    Keywords: 42.10 ; 42.80
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    Topics: Physics
    Notes: Abstract Two rival techniques used in the analysis of Bragg diffraction of two-dimensional light beams by thick unslanted phase gratings, namely Fourier plane wave decomposition and 2-D coupled-wave theory, are compared. The advantages and regions of applicability of each are discussed, and the conditions found under which they yield identical results. In particular, the results of plane-wave decomposition are used to provide quantitative conditions for validity of 2-D coupled-wave theory. These conditions are not easily derived using any other technique, and set quantitative limits to how narrow or fast-varying the incident distribution may be before 2-D coupled-wave theory fails. It is also shown that 2-D coupled-wave theory is inadequate when the parameterv 0, due to Kogelnik (1969), which is proportional to the product of coupling rate and grating thickness, is very large.
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    Applications of ps-pulses (1982)
    Springer
    Applied physics 28 (1982), S. 246-254 
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    Keywords: 42.80 ; 42.65 ; 34 ; 42.55 ; 42.70 ; 78
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    Topics: Physics
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    Theory II (1982)
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    Applied physics 28 (1982), S. 255-261 
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    Keywords: 42.55 ; 36.20 ; 36.10 ; 02 ; 36 ; 42.80
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    Coherent transient phenomena (1982)
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    Applied physics 28 (1982), S. 274-282 
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    Keywords: 42.80 ; 33 ; 36 ; 36.10 ; 14 ; 32
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    Topics: Physics
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    A novel method of DNA transfection by laser microbeam cell surgery (1984)
    Springer
    Applied physics 35 (1984), S. 135-140 
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    ISSN: 1432-0649
    Keywords: 07.60 ; 42.80 ; 87
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    Topics: Physics
    Notes: Abstract A new technique is presented to incorporate exogeneous gene materials (DNA) into cells with a microbeam irradiation from an uv pulsed laser. A frequency-multiplied Nd:YAG laser, 355 m wavelength, 5 ns pulse duration, punches a self-healing hole of submicrometer aperture in cell membrane under selected irradiation conditions. It takes a fraction of a second for the aperture to close, long enough to allow the foreign DNA, contained in the medium, to slip into the cell. The method offers a clear advantage over existing methods: increases the success rate of DNA transfection as well as the efficiency of cell modification by orders of magnitude.
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    Faraday rotation in a single-mode fiber with controlled birefringence (1984)
    Springer
    Applied physics 35 (1984), S. 145-148 
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    Keywords: 42.80 ; 42.10
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    Topics: Physics
    Notes: Abstract Faraday rotation within a polarization non-conserving single-mode optical fiber is used for ac magnetic field measurement. For that purpose the intrinsic fiber birefringence on the path to and from the Faraday rotation section is controlled. As control devices externally applied stress birefringence and phase retarder plates were used. With respect to magnetic field measurements on high electric potentials all control devices are placed at fiber input and output only.
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    New lasers II (1982)
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    Applied physics 28 (1982), S. 233-239 
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    ISSN: 1432-0649
    Keywords: 42.70 ; 42.55 ; 42.80
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    Topics: Physics
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    Laser fusion (1982)
    Springer
    Applied physics 28 (1982), S. 269-273 
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    ISSN: 1432-0649
    Keywords: 52 ; 42.55 ; 42.80 ; 51
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    Topics: Physics
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    Time delays in lead-salt semiconductor diode lasers (1984)
    Springer
    Applied physics 33 (1984), S. 181-182 
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    ISSN: 1432-0630
    Keywords: 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Time delays of typically 15–17μ have been measured directly for PbS1−xSex, Pb1−xSnxSe and Pb1−xSnxTe diode lasers at injection levels just above threshold in each case. The corresponding minority carrier lifetimes, as determined using the one-carrier injection model, were typically 2–4μ.
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    The dielectric constant of PbTe at 4.2 K and $$\tilde v$$ =84.15 cm-1, 96.97cm−1, 103.60cm−1 (1982)
    Springer
    Applied physics 28 (1982), S. 229-233 
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    ISSN: 1432-0630
    Keywords: 77 ; 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The dielectric constant of a PbTe epitaxial layer has been measured by surface wave spectroscopy using an optically pumped far-infrared laser and the technique of attenuated total reflection.
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    Photoconducting tellurium for submillimeterwave detectors (1982)
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    Applied physics 27 (1982), S. 35-38 
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    ISSN: 1432-0630
    Keywords: 42.80 ; 72.40 ; 78.20
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Undoped tellurium single crystals show at low temperatures (2.2 K) strong photoeffect under submillimeter irradiation. Therefore, tellurium was used as a FIR-detector. For a comparison with other detectors signal-to-noise ratio, response time, and noise equivalent power were determined. Tests were carried out with HCN-laser radiation (λ = 337 μm,≈ν = 30 cm- t). The sensitivity of the Tc-photoconductor is comparable to that of a TGS-bolometer but lower than that of a Ge-bolometer. The Te-detector is much faster than the bolometers.
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    Optical method for measuring the coupling constant of surface acoustic waves (1982)
    Springer
    Applied physics 27 (1982), S. 95-97 
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    ISSN: 1432-0630
    Keywords: 42.80 ; 62
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The coupling constant of common or thin-layered-piezoelectric interdigital transducers for surface acoustic waves up to the highest frequencies can be determined in an optical way with reproducibility better than 3%. Being a nondestructive and noncontact method one can apply it in the technological process for fabricating uniform piezoelectric layered transducers.
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    Space-time resolved reflectivity measurements of picosecond laser-pulse induced phase transitions in (111) silicon surface layers (1982)
    Springer
    Applied physics 27 (1982), S. 153-160 
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    ISSN: 1432-0630
    Keywords: 78.20 ; 81 ; 42.80
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The changes in reflectivity of a silicon surface, irradiated by a green picosecond pulse, are probed during and following that pulse with a spatial resolution of 10 μm. The data indicate the development of a liquid phase, and a resolidification either into a single crystal or an amorphous phase. The latter has a characteristic ring-type pattern, and occurs only at locations where the incident picosecond laser fluence lies between 0.2 and 0.26 J/cm2. The reflectivity data appear to be in good quantitative agreement with a “simple heating” model, in which the electrons and phonons maintain a local thermodynamic equilibrium on a picosecond time scale.
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    Radiation sensitivity of thick core all-glass fibers in reactor radiation fields (1982)
    Springer
    Applied physics 28 (1982), S. 45-51 
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    ISSN: 1432-0630
    Keywords: 42.10 ; 42.80 ; 78.20
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Radiation induced losses and the recovery behavior of several optical fibers have been determined by in situ steady state and by pulse measurements in a nuclear reactor. Agreement with the literature was found for Suprasil and Fibropsil core fibers in ionizing radiation, but our results on Suprasil-W core fibers show an appreciably higher radiation hardness of this material than reported elsewhere. Additionally, increased long term losses were observed, when the mixed radiation flux contained a noticeable content of neutrons.
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    Mutations affecting the pattern of the larval cuticle inDrosophila melanogaster (1984)
    Springer
    Development genes and evolution 193 (1984), S. 267-282 
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    ISSN: 1432-041X
    Keywords: Drosophila ; Larval cuticle ; Pattern formation ; Embryonic lethal mutations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In a search for embryonic lethal mutants on the second chromosome ofDrosophila melanogaster, 5764 balanced lines isogenic for an ethyl methane sulfonate (EMS)-treatedcn bw sp chromosome were established. Of these lines, 4217 carried one or more newly induced lethal mutations corresponding to a total of 7600 lethal hits. Eggs were collected from lethal-bearing lines and unhatched embryos from the lines in which 25% or more of the embryos did not hatch (2843 lines) were dechorionated, fixed, cleared and scored under the compound microscope for abnormalities of the larval cuticle. A total of 272 mutants were isolated with phenotypes unequivocally distinguishable from wild-type embryos on the basis of the cuticular pattern. In complementation tests performed between mutants with similar phenotype, 48 loci were identified by more than one allele, the average being 5.4 alleles per locus. Complementation of all other mutants was shown by 13 mutants. Members of the complementation groups were mapped by recombination analysis. No clustering of loci with similar phenotypes was apparent. From the distribution of the allele frequencies and the rate of discovery of new loci, it was estimated that the 61 loci represent the majority of embryonic lethal loci on the second chromosome yielding phenotypes recognizable in the larval cuticle.
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    Isolation and characterization of temperature-sensitivelethal (2) giant larva alleles (1984)
    Springer
    Development genes and evolution 193 (1984), S. 90-97 
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    ISSN: 1432-041X
    Keywords: Drosophila ; Temperature-sensitive ; Neoplasms ; Differentiation ; Imaginal discs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary EMS induced temperature-sensitivelethal (2) giant larva, 1(2)gl, alleles were isolated by screening against a knownl(2)gl allele. Analysis of the lethal phase of thel(2)gl ts-deficiency heterozygotes demonstrated: (1) the majority of thel(2)gl tslarvae survive to late third instar, (2) at 29°C the majority of thel(2)gl tslarvae failed to pupate and only rarely did they differentiate adult cuticular structures, (3) at 15°C the majority of the larvae pupated and frequently differentiated adult cuticular structures. Examination of the imaginal discs ofl(2)gl tslarvae reared at 29°C revealed the presence of morphologically abnormal wing, haltere and leg imaginal discs. No morphologically abnormal discs were found in thel(2)gl tslarvae reared at 15°C. Studies on both the histology and the developmental capacity of the morphologically normal and abnormall(2)gl tsdiscs were performed. The morphologically normal discs are histologically normal and produce a full complement of adult cuticular structures. However, the morphologically abnormal discs contained both regions that maintained the normal monolayer epithelium and regions that had lost the normal tissue architecture. The implants obtained when the morphologically abnormal discs are injected into metamorphosing larvae contained only a limited number of the normal complement of adult structures and usually only structures found in the ventral wing hinge region were recovered. In addition, the “metamorphosed” morphologically abnormal discs contained undifferentiated tissue that gave rise to transplantable neoplasms when cultured in adults. The results of the studies on the pathology of thel(2)gl tslarvae are discussed with respect to the role of thel(2)gl tsfunction during normal development, the autonomy of the neoplastic development of thel(2)gl tstissues, and similarities between neoplastic development inDrosophila and mammals.
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    Heterospecific combinations of germ cells and gonadal soma betweenDrosophila melanogaster, D. mauritiana andD. ananassae (1984)
    Springer
    Development genes and evolution 194 (1984), S. 99-106 
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    ISSN: 1432-041X
    Keywords: Drosophila ; Pole cell transplantation ; Heterospecific combinations ; Gametogenesis ; Chorion morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We transplanted pole cells betweenDrosophila melanogaster, D. mauritiana andD. ananassae to investigate the ability of germ cells to develop in the gonad of a heterospecific host, and to study the interaction between somatic follicle cells and the cells of the germ line in producing the species-specific chorion. FemaleD. mauritiana germ cells in aD. melanogaster ovary produced functional eggs with normal development potential. The same is true for the reciprocal combination. FemaleD. ananassae pole cells in aD. melanogaster host only developed to a very early stage and degenerated afterwards. None of the interspecific combinations of male pole cells led to functional sperm. We could not determine at what stage the transplanted male pole cells were arrested. The cooperation of follicle cells and the oocyte-nurse cell complex in producing the chorion was studied using the germ-line-dependent mutationfs(1) K10 ofD. melanogaster, which causes fused respiratory appendages and an abnormal chorion morphology. Wild-type femaleD. mauritiana germ cells in a mutantfs(1) K10 D. melanogaster ovary led to the production of wild-type eggs withD. melanogaster-specific, short respiratory appendages. On the other hand,D. melanogaster fs(1) K10 germ cells in aD. mauritiana ovary induced the formation of eggs with mutant fused appendages which were, however, typicallyD. mauritiana in length. When.D. mauritiana pole cells developed in aD. melanogaster ovary, the chorion exhibited a new imprint pattern that differs from both species-specific patterns.
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    Correlation between adult transformation and aldehyde oxidase staining pattern in wing discs ofApterous genotypes inDrosophila melanogaster (1982)
    Springer
    Development genes and evolution 191 (1982), S. 285-288 
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    ISSN: 1432-041X
    Keywords: Drosophila ; Imaginal discs ; homoeosis ; Compartments ; Aldehyde oxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The aldehyde oxidase staining pattern in wing discs ofDrosophila melanogaster bearing the genotypesap blt /ap blt andap blt andap blt /ap 73n showns changes from the wild-type pattern. Extensive areas of the presumptive dorsal posterior wing blade, which are normally unstained, have enzyme activity in these mutants. In wings of these genotypes, dorsal posterior structures are replaced by dorsal anterior wing structures. A strong correlation has been found between the frequencies of various staining patterns in the discs and the extent of transformation in the cuticular structures of the wing, which is consistent with the idea that aldehyde oxidase activity can be used as an indicator in the wing disc of this transformation. Unlike the homoeotic mutationengrailed, apterous has not been interpreted as a selector gene yet the work reported here shows thatapterous alleles can cause changes resembling those of theengrailed phenotype both in aldehyde oxidase staining behaviour and in the cuticular transformation.
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    Effects of the “sexcombless” chromosome (In(1)sx) on males and females ofDrosophila melanogaster (1982)
    Springer
    Development genes and evolution 191 (1982), S. 264-269 
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    ISSN: 1432-041X
    Keywords: Drosophila ; Sexcombless ; Foreleg basitarsus ; Genital disc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The chromosome which carries the mutationsexcombless (In(1)sx) affects males and females ofD. melanogaster. In the male foreleg basitarsi the number of sexcomb teeth is dramatically reduced from 10 to 0.7 and the number of transverse rows of bristles is increased from 6 to 8. Females homozygous forIn(1)sx show a normal bristle pattern in the foreleg basitarsus. The genital disc derivatives of both male and femaleIn(1)sx flies are strongly affected. While the external genitalia show a duplicated or a reduced bristle pattern, the internal genitalia are mostly absent. However, the sexually dimorphic tergites and sternites of the abdomen remain unaffected. The male-specific effect on the basitarsus and the general effects on the genital disc derivatives are proposed to represent two different phenotypic effects ofIn(1)sx which may derive from mutations at different gene loci in the inverted chromosome.
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    URL: http://dx.doi.org/10.1007/BF00848414
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    Growth is required for cell competition in the imaginal discs ofDrosophila melanogaster (1982)
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    Development genes and evolution 191 (1982), S. 289-291 
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    ISSN: 1432-041X
    Keywords: Drosophila ; Imaginal discs ; Cell competition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Imaginal wing discs from late third-instar larvae were gammairradiated to induce clones of rapidly growingMinute − cells in a background of slowly growingMinute cells and culturedin vivo for periods up to 18 days. Clones in discs cultured for 16 to 18 days did not grow significantly larger than clones in uncultured controls, indicating that competition between populations of cells having potentially different mitotic rates does not occur in imaginal discs after their growth is completed.
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    Isolation and characterization of temperature-sensitivelethal(2)giant larva alleles (1984)
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    Development genes and evolution 193 (1984), S. 98-107 
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    ISSN: 1432-041X
    Keywords: Drosophila ; Neoplasms ; Promotion ; Regeneration ; Temperature-sensitive ; Imaginal discs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In this paper we present an analysis of the behavior ofl(2)gl tsimaginal wing discs during culture in adult hosts. Thel(2)gl tslarvae reared at 29° C contain two types of wing discs, those that are morphologically normal and those that are abnormal. When discs of both types are cultured in adult hosts at 29° C, the restrictive temperature, they give rise to transplantable neoplastic tissue. However, when the 29° C reared discs are cultured at 15° C, the permissive temperature, the morphologically normal discs maintain their morphology, but the morphologically abnormal discs give rise to neoplasms. Thel(2)gl tslarvae reared at 15° C contain only morphologically normal discs. When these discs are cultured in adult hosts at 29° C they give rise to neoplasms, however if the discs are cultured at 15° C they maintain their normal morphology. These results demonstrate: (1) that all wing imaginal discs obtained from 29° C rearedl(2)gl tslarvae are competent to undergo neoplastic development, (2) the morphologically abnormal discs obtained from the 29° C rearedl(2)gl tslarvae are committed to neoplastic development, (3) the neoplastic development of the morphologically normal discs is temperature dependent, (4) once the neoplastic development of thel(2)gl tsdiscs has been initiated the process is not readily reversible. In addition, the ability ofl(2)gl tswing discs to perform epimorphic regulation was tested by amputating morphologically normal permissively rearedl(2)gl tswing discs and culturing both fragiments at the permissive temperature. Fragments of control wild-type discs maintained their morphology during culture at the permissive temperature. However, both fragments of txel(2)gl tsdiscs became neoplastic. This result is discussed with respect to a possible role for thel(2)gl +function in epimorphic regulation and with respect to the phenomena of tumor promotion in vertebrates.
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    Sequential gene activation by ecdysteroids in polytene chromosomes ofDrosophila melanogaster (1982)
    Springer
    Development genes and evolution 191 (1982), S. 103-111 
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    ISSN: 1432-041X
    Keywords: Drosophila ; Polytene Chromosomes ; Ecdysteroids ; Fat Body
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Changes in polytene chromosome 3 L puffing patterns in the fat body ofDrosophila melanogaster larvae and prepupae are compared to those in the salivary gland. While some general features are common to the two tissues, there are differences which reflect their different developmental roles. In vitro experiments with fat body chromosomes show that they have a distinct response to ecdysteroids which is different from that of salivary gland chromosomes, and which does not,in this culture system, reproduce the changes observed in normal development. In short term culture experiments, the fat body chromosomes appear more sensitive to ecdysteroids than the salivary gland chromosomes and, although 20-OH ecdysone is more active than ecdysone in these assays, the possibility is not excluded that ecdysone has a role in normal development as it appears to alter gene activity at physiological levels in these cells.
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    Homologies of positional information in thoracic imaginal discs ofDrosophila melanogaster (1982)
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    Development genes and evolution 191 (1982), S. 293-300 
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    ISSN: 1432-041X
    Keywords: Drosophila ; Imaginal discs ; Positional information ; Homology ; Intercalary regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The regulative behavior of fragments of the imaginal discs of the wing and first leg was studied when these fragments were combined with fragments of other thoracic imaginal discs. A fragment of the wing disc which does not normally regenerate when cultured could be stimulated to regenerate by combination with certain fragments of the haltere disc. When combined with a haltere disc fragment thought to be homologous by the criteria of morphology and the pattern of homoeotic transformation, such stimulated intercalary regeneration was not observed. Combinations of first and second leg disc fragments showed that a lateral first leg fragment could be stimulated to regenerate medial structures when combined with a medial second leg disc fragment but not when combined with a lateral second leg disc fragment. Combinations of wing and second leg disc fragments showed that one fragment of the second leg disc is capable of stimulating regeneration from a wing disc fragment while another second leg disc fragment fails to stimulate such regeneration. It is suggested that absence of intercalary regeneration in combinations of fragments of different thoracic imaginal discs is a result of homology or identity of the positional information residing in the cells of the fragments. The pattern of correspondence of positional information revealed by this analysis is consistant with the pattern of homology determined by morphological observation and by analysis of the positional specificity of homoeotic transformation among serially homologous appendages. The implications of the existence of homologous positional information in wing and second leg discs which share a common cell lineage early in development are discussed.
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    Gap junctions are non-randomly distributed inDrosophila wing discs (1982)
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    Development genes and evolution 191 (1982), S. 335-339 
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    ISSN: 1432-041X
    Keywords: Drosophila ; Gap junction ; Wing disc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The distribution of gap junctions in mature larvalDrosophila melanogaster wing discs was analyzed by means of quantitative electron microscopy. Gap junctions are non-randomly distributed in the proximal-distal disc axis and in the apical-basal cell axis of the epithelium. In the epithelial cells, the surface density, number and length of gap junctions are greatest in the apical cell region and distal disc region. The average gap junction surface density is 0.0572 μm−1 and 2.77% of the lateral cell surface is composed of gap junctions. In the adepithelial cells, the gap junction surface density is 0.0005 μm−1 and 0.06% of the cell surface is composed of gap junctions. No gap junctions were observed between epithelial cells and adepithelial cells. The absolute area of gap junctions was estimated in a proximal-distal strip of cells in the disc and is considerably less in the folded regions of the epithelium compared to the flat notum and wing pouch regions. The results are discussed with respect to pattern formation and growth control in imaginal discs.
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    Dicephalic — ADrosophila mutant affecting polarity in follicle organization and embryonic patterning (1982)
    Springer
    Development genes and evolution 191 (1982), S. 28-36 
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    ISSN: 1432-041X
    Keywords: Drosophila ; Polarity ; Maternal effect ; Nurse cells ; Embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mutationdicephalic (dic) affects follicle development and thereby alters the antero-posterior polarity of embryonic patterning. It maps at a single locus (3–46.0±1.0) and can be characterized as a semi-dominant maternal effect mutation with low penetrance. Indic follicles, the 15 nurse cells form two clusters located at opposite poles of the oocyte; the numerical distribution of the nurse cells among the clusters varies from 7:8 to 1:14. Thedic egg shell carries a micropyle (anterior marker) at either pole, but the misshapen respiratory appendages are restricted to one of the two poles in most eggs. The malformed eggs rarely yield larvae and these are always abnormal anteriorly and/or posteriorly. The segment pattern expressed in their cuticle may represent two anterior parts of opposite polarities (double head type), two posterior parts of opposite polarities (double abdomen type, rare) or show uniform polarity. Lability of organization at the cystocyte stage appears as the primary developmental defect of the mutant.
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    Changes in the distribution of gap junctions inDrosophila melanogaster wing discs during the third larval and early pupal stages of development (1984)
    Springer
    Development genes and evolution 193 (1984), S. 187-196 
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    ISSN: 1432-041X
    Keywords: Drosophila ; Gap junction ; Imaginal disc ; Pattern formation ; EM Stereology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Developmental changes in the distribution of gap junctions in early, mid and late third larval stage wing discs and in pupariation+6 h and pupariation+24 h stage wing discs fromDrosophila melanogaster were analyzed by quantitative electron microscopy. Gap junctions occur in all 12 intradisc regions examined in each of the five developmental stages. Their distribution is non-random and changes during development which suggests that they are developmentally regulated. The gap junctions are not static structures, rather they grow and regress during development. The changes tend to be gradual ones without sudden increases or decreases. Gap junctions continuously form and grow in size throughout the third larval stage and during the first 6 h following pupariation. Their surface density, number, percent of the lateral plasma membrane area, and absolute area as well as the lateral plasma membrane surface density all increase during this time. Between pupariation+ 6 h and pupariation+24 h all but one of these parameters decrease indicative of gap junctional breakdown. Gap junctions are most numerous and change least during development in the apical cell regions where intercellular contacts are close and stable. They change most in the basal cell regions where intercellular contacts tend to be looser and change during development. The most dramatic change is in the absolute area which increases by a factor of 23 between the early third larval stage and pupariation+24 h. At pupariation the rate of gap junction growth undergoes a transient increase before the phase of disassembly begins. Developmental changes in gap junction surface density are closely coupled with changes in the lateral plasma membrane surface density which suggests that these may be coregulated. Evidence from mutants suggests that when the number and density of gap junctions fail to increase in proportion to lateral plasma membrane growth, wing disc development will be abnormal. Our results support the idea that some minimum gap junction density is required for normal development and that this must increase as development proceeds. The results are consistent with the notion that gap junctions are involved in pattern formation and growth control and are discussed with respect to the acquisition of competence for metamorphosis, disc growth, disc morphogenesis and changes in the hormonal environment.
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    Immunocytochemical and electrophoretic studies on the localization of the major haemolymph proteins (ceratitins) inCeratitis capitata during development (1984)
    Springer
    Development genes and evolution 194 (1984), S. 37-43 
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    ISSN: 1432-041X
    Keywords: Major haemolymph proteins ; Development ; Cuticle ; Immunocytochemistry ; Ceratitis capitata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The developmental profile of the major haemolymph proteins (ceratitins) inCeratitis capitata was studied. Ceratitin concentration in the haemolymph decreases dramatically during the last days of pupal life, while the amounts of ceratitins in whole organism extracts remain unchanged. By electrophoretic, immunological and immunofluorescence techniques it was revealed that ceratitins are reabsorbed by the fat body and a fraction of them is deposited in the cuticle. The possible role of ceratitins is discussed.
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    A clonal analysis of cell lineage and growth in the male and female genital disc ofDrosophila melanogaster (1982)
    Springer
    Development genes and evolution 191 (1982), S. 42-55 
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    ISSN: 1432-041X
    Keywords: Clonal analysis ; Growth ; Cell lineage ; Genital disc ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary InDrosophila, the terminalia (i.e. internal and external analia and genitalia, except the gonads) are formed by the genital disc. Comparative studies suggested that this disc may have evolved through fusion of the imaginal primordia of the last 3 or 4 abdominal segments. The present report describes the clonal relationships within the complex genital disc. Genetically marked cell clones were induced in male and female embryos and larvae heterozygous for cell marker mutations. 1) Frequencies and sizes of clones suggest that the embryonic disc anlage consists of 14–17 precursor cells: 4–6 for the analia, some 7 for the male genitalia, and 3–4 for the female genitalia. These cells grow exponentially during larval development. 2) In both sexes, the clones were confined to either analia or genitalia, suggesting two separate cell lineages already established at blastoderm. 3) Internal and external genitalia remain in the same compartment at least up to 60 h (end of first instar). 4) A clonal restriction appeared around 84 h (mid second instar), separating a dorsal from a ventral part in the male genitalia. The ventral compartment comprises the ventral part of the lateral plate and clasper, hypandrium, and all internal genitalia. No such boundary was detected in the female. 5) In the female, analia and parovaria originate from the same precursors; another cell lineage forms eighth tergites, vaginal plates, oviduct, receptacle, and spermathecae. 6) In female analia, dorsal and ventral plate share common precursors at least up to 84 h. A medio-lateral boundary may appear at 84 h in the ventral anal plate. No clonal restriction was found in the male analia. 7) At all times, clones could cross between left and right sides of the symmetrical terminalia; they consistently did so via ventral structures. 8) The results are discussed in a phylogenetic context, and we propose that the clonal relations reflect the evolution of the complex genital disc.
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    Maternal effects of zygotic mutants affecting early neurogenesis inDrosophila (1982)
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    Development genes and evolution 191 (1982), S. 191-201 
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    ISSN: 1432-041X
    Keywords: Neurogenic mutants ; Maternal effects ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The size of the neurogenic region ofDrosophila melanogaster is under the control of several genes of zygotic expression. Lack of function from any of those genes produces an increase of the size of the neurogenic region at the expense of the epidermal anlage. However, differences exist in the extent of neuralisation achieved by each of the genetic loci upon mutation. The present results show that in the case ofN andmam phenotype differences are due to different contributions of maternal gene expression. This could be shown by studying the phenotype which appeared in mutant embryos when the oocytes developed from homozygous mutant precursor cells. Clones of mutant cells were induced in the germ line of females heterozygous for the neurogenic mutationin trans over germ line dependent, dominant female sterile mutations. After removing maternal information the phenotype ofN andmam mutants became identical in both cases. Furthermore maternal information fromN + was found to be necessary for viability of the wildtype.
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    Clonal analysis ofsex-lethal, a gene needed for female sexual development inDrosophila melanogaster (1982)
    Springer
    Development genes and evolution 191 (1982), S. 211-214 
    Details
    ISSN: 1432-041X
    Keywords: Drosophila ; Clonal analysis ; Sex determination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mutationSxl f , located on the X-chromosome, is a sex-limited recessive lethal that specifically kills 2X; 2A flies while it does not affect X; 2A flies (Cline 1978). We have analyzed the role ofSxl f on sex determination by a clonal analysis of a new spontaneous allele,Sxl fLS . Female embryos and larvae heterozygous forSxl fLS were irradiated at different times of development to generate homozygousSxl fLS clones which were recognized by linked marker mutations. We have studied the phenotype of such clones on sexually dimorphic regions of the fly (foreleg basitarsus, 5th, 6th and 7th tergites, analia and external genitalia). Despite their female (2X; 2A) chromosomal constitution, clones homozygous forSxl fLS differentiated male structures. These results confirm and extend the preliminary report of Cline (1979). They show that the wildtype product ofSxl f is required for female development.
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    Mutations affecting the pattern of the larval cuticle inDrosophila melanogaster (1984)
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    Development genes and evolution 193 (1984), S. 296-307 
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    ISSN: 1432-041X
    Keywords: Drosophila ; Larval cuticle ; Pattern formation ; Embryonic lethal mutations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In order to identify X-chromosomal genes required inDrosophila for early patterning and morphogenesis, we examined embryos hemizygous for EMS-induced lethal mutations to determine which of those mutations cause gross morphological defects. Embryos from 2711 lethal lines, corresponding to 3255 lethal point mutations were studied. Only 21% caused death during embryogenesis and of these, only one-sixth, or 3% of the total lethals, were associated with defects visible in the final cuticle pattern. Of the 114 point mutants causing visible cuticle defects, 76 could be assigned to 14 complementation groups. An additional 25 mutations mapping to regions of the X-chromosome not covered by male fertile duplications were assigned to six complementation groups based on similarities of map position and phenotype. Thirteen mutations could not be assigned to complementation groups. All mutations allowed normal development through the cellular blastoderm stage, the first defects associated with the earliest acting loci being observed shortly after the onset of gastrulation. The phenotypes of the various loci range from alterations in segment pattern or early morphogenetic movements to defects in final pigmentation and denticle morphology. Cuticle preparations were also examined for 63 deletions spanning in total 74% of the X-chromosome, as well as for 8 deletions and point mutations derived in saturation mutagenesis screens of the fourth chromosome (Hochman 1976). With the exception of defects in head morphology and defects in cuticle differentiation, none of the hemizygous deletions showed phenotypes other than those predicted by point mutations known to lie in those regions. No deletion caused new or unknown alterations in gastrulation, segmentation or cuticle pattern.These results suggest that the number of genes required zygotically for normal embryonic patterning is small and that most, if not all such loci, are represented by point mutations in our collection.
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    The role of the peripodial membrane of leg and wing imaginal discs ofDrosophila melanogaster during evagination and differentiation in vitro (1984)
    Springer
    Development genes and evolution 193 (1984), S. 180-186 
    Details
    ISSN: 1432-041X
    Keywords: Drosophila ; Imaginal disc ; Morphogenesis ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The thin region of the peripodial membrane is confined to the area overlying the distal anlagen in thoracic discs. During the early stages of evagination the peripodial membrane is greatly stretched, but does not rupture. The appendage then evaginates through the stalk, probably by means of a contraction of the peripodial membrane. The cells of the peripodial membrane of leg and wing discs persist and differentiate sheets of trichomes characteristic of the ventral and lateral thorax. This is discussed in relation to imaginal disc fate maps.
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    TheUbx syndrome ofDrosophila: the prothoracic transformation (ppx) is independent ofbx, bxd andpbx (1984)
    Springer
    Development genes and evolution 193 (1984), S. 263-265 
    Details
    ISSN: 1432-041X
    Keywords: Drosophila ; Biothorax complex ; Prothoracic transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary If, early in development, theUbx + gene is removed by mitotic recombination from cells of the meso-and metathoracic leg primordia, theseUbx − cells develop as in the posterior prothoracic leg. We show that this transformation, termedpostprothorax, is a discrete genetic function that is independent of other homeotic transformations such asbx, pbx orbxd, which also result from the inactivation of theUbx gene.
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    Mutations affecting the pattern of the larval cuticle inDrosophila melanogaster (1984)
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    Development genes and evolution 193 (1984), S. 283-295 
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    ISSN: 1432-041X
    Keywords: Drosophila ; Larval cuticle ; Pattern formation ; Embryonic lethal mutations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The present report describes the recovery and genetic characterization of mutant alleles at zygotic loci on the third chromosome ofDrosophila melanogaster which alter the morphology of the larval cuticle. We derived 12600 single lines from ethyl methane sulfonate (EMS)-treatedst e orrucuca chromosomes and assayed them for embryonic lethal mutations by estimating hatch rates of egg collections. About 7100 of these lines yielded at least a quarter of unhatched eggs and were then scored for embryonic phenotypes. Through microscopic examination of unhatched eggs 1772 lines corresponding to 24% of all lethal hits were classified as embryonic lethal. In 198 lines (2.7% of all lethal hits), mutant embryos showed distinct abnormalities of the larval cuticle. These embryonic visible mutants define 45 loci by complementation analysis. For 32 loci, more than one mutant allele was recovered, with an average of 5.8 alleles per locus. Complementation of all other mutants was shown by 13 mutants. The genes were localized on the genetic map by recombination analysis, as well as cytologically by complementation analysis with deficiencies. They appear to be randomly distributed along the chromosome. Allele frequencies and comparisons with deficiency phenotypes indicate that the 45 loci represent most, if not all, zygotic loci on the third chromosome, where lack of function recognizably affects the morphology of the larval cuticle.
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    Early neurogenesis in wild-typeDrosophila melanogaster (1984)
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    Development genes and evolution 193 (1984), S. 308-325 
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    ISSN: 1432-041X
    Keywords: Neurogenesis ; Pattern of neuroblasts ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary This paper deals with morphological aspects of early neurogenesis inDrosophila, in particular with the segregation of neuroblasts from the neurogenic region of the ectoderm and the pattern formed by those wells within both the germ band and the procephalic lobe. The neurogenic ectoderm was found to contain neural precursors intermingled with epidermal precursors, extending from the midline up to the primordia of the tracheal tree along the germ band and laterodorsally in the procephalic lobe. Germ band neuroblasts segregate from the neurogenic ectoderm during a period of several hours according to characteristic spatial and temporal patterns. During the first half of the segregation process the pattern of germ band neuroblasts was found to be the same in different animals in both spatial arrangement and number of cells; this permitted the identification of individual neuroblasts from different embryos. Later in development several difficulties were encountered which precluded an exact description of the neuroblast pattern. The constitution of the neurogenic region is discussed in relation to the phenotype of mutants affecting neurogenesis.
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    The spatial arrangement of germ line cells in ovarian follicles of the mutantdicephalic inDrosophila melanogaster (1984)
    Springer
    Development genes and evolution 193 (1984), S. 388-393 
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    ISSN: 1432-041X
    Keywords: Drosophila ; Oogenesis ; Ring canals ; Oocyte determination ; Polarity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The pattern of intercellular connections between germ line cells has been studied in follicles of the mutantdicephalic (dic), which possess nurse cell clusters at both poles. Staining of follicles with a fluorescent rhodamine conjugate of phalloidin reveals ring canals and cell membranes and thus allows us to reconstruct the spatial organization of the follicle. Each germ line cell can be identified by the pattern of cell-cell connections which reflect the mitotic history of individual cells in the 16-cell cluster. The results indicate that in both wild-type anddicephalic cystocyte clusters one of the two cells with four ring canals normally becomes the pro-oocyte. However, in some follicles (dicephalic and wild-type) oocytes were found with fewer or more than four ring canals. Indic follicles, one or several nurse cells may become disconnected from the other cells during oocyte growth at stage 9–10. Such disconnected cells cannot later on empty their cytoplasm into the oocyte. This, in turn, might be of consequence for the determination of axial polarity of the embryo.
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    Cell interactions during the fusionin vitro ofDrosophila eye-antennal imaginal discs (1984)
    Springer
    Development genes and evolution 193 (1984), S. 406-413 
    Details
    ISSN: 1432-041X
    Keywords: Drosophila ; Imaginal disc ; Morphogenesis ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The fusion of the eye-antennal discs during culturein vitro has been investigated, and the complex morphogenetic movements which occur during the formation of the head capsule of the insect are described. The initial contact between the eye anlagen is by means of cell processes spanning the gap between the two discs. Subsequently the two epithelia become firmly apposed, and then the integrity of the epithelium in the region of fusion breaks down, cells appearing to move to new positions in order to form an epithelium which unites the two discs. The epithelium eventually secretes a pattern of cuticular structures which is continuous between the derivatives of the two discs. Bristles on either side of the line of fusion are perfectly aligned, and structures such as the median ocellus, which are formed jointly by the cells of the two discs, differentiate normally. This is also found when left and right eye-antennal discs of different genotypes are placed side-by-side, indicating that processes of pattern regulation can occur in culture.
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    Localization of phytohemagglutinin in the embryonic axis of Phaseolus vulgaris with ultra-thin cryosections embedded in plastic after indirect immunolabeling (1984)
    Springer
    Planta 162 (1984), S. 548-555 
    Details
    ISSN: 1432-2048
    Keywords: Immunocytochemistry ; Lectin (localization) ; Phaseolus (lectin) ; Phytohemagglutinin ; Seed (lectin)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have examined the properties and subcellular localization of phytohemagglutinin (PHA), the major lectin of the common bean (Phaseolus vulgaris.), in the axis cells of nearly mature and imbibed mature seeds. On a protein basis the axis contained about 15% as much PHA as the cotyledons. Localization of PHA was done with an indirect immunolabeling method (rabbit antibodies against PHA, followed by colloidal gold particles coated with goat antibodies against rabbit immunoglobulins) on ultra-thin cryosections which were embedded in plastic on the grids after the immunolabeling procedure. The embedding greatly improved the visualization of the subcellular structures. The small (4 nm) collodial gold particles, localized with the electron microscope, were found exclusively over small vacuoles or protein bodies in all the cell types examined (cortical parenchyma cells, vascular-bundle cells, epidermal cells). The matrix of these vacuoles-protein bodies appears considerably less dense than that of the protein bodies in the cotyledons, but the results confirm that in all parts of the embryo PHA is localized in similar structures.
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    Tissue-specific and substrate-specific detection of aldehyde and pyridoxal oxidase in larval and imaginal tissues of Drosophila melanogaster (1982)
    Springer
    Biochemical genetics 20 (1982), S. 315-332 
    Details
    ISSN: 1573-4927
    Keywords: aldehyde oxidase ; pyridoxal oxidase ; tissue specificity ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The substrate specificities of aldehyde and pyridoxal oxidases in Drosophila melanogaster have been determined with a variety of aliphatic and aromatic aldehydes. This analysis has led to the discovery that 2,4,5-trimethoxy-benzaldehyde is a specific substrate for pyridoxal oxidase, as based on the histochemical distribution of oxidase activity, the absence of enzymatic activity in the lpo 1strains, and the dosage dependence on the number of lpo +genes present. The tissue-specific localization of aldehyde oxidase (AO) and pyridoxal oxidase (PO) in the larval and adult structures showed that AO was present in all the major internal organs of the larvae and adults, including brain, imaginal discs, Malpighian tubules, digestive system, and reproductive structures. Pyridoxal oxidase is present in many of the same structures which possess AO, but is missing from the cardia, crop, imaginal discs, ovarian follicle cells, paragonia, pericardial cells, and wreath cells. The only structure which possesses PO but lacks AO is the larval salivary gland. These histochemical differences in AO and PO distribution were also confirmed by enzymatic analysis of the activities present in homogenates of ovaries, paragonia, and salivary glands. The general pattern of enzyme expression appears to be established during embryogenesis and maintained throughout the life of the individual.
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    Selection at the α-Gpdh locus in experimental populations of Drosophila melanogaster (1982)
    Springer
    Biochemical genetics 20 (1982), S. 461-474 
    Details
    ISSN: 1573-4927
    Keywords: Drosophila ; allozymes ; α-Gpdh ; selection ; genetic background
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Three sets of experiments have been conducted in order to evaluate the role of natural selection at the α-Gpdh locus in Drosophila melanogaster. (1) The evolution of the F-allele frequency has been followed for many generations in 13 experimental populations having different genetic backgrounds. (2) Egg-to-adult viability has been measured in synthetic populations derived from one locality (Brouilly) and the results have been compared with those of a previous experiment involving a different local population (Tostes). (3) The effects of sodium octanoate on egg-to-adult viability have been measured on the genotypes FF, FS, SF, and SS. The results demonstrate that selection operates on a small block of genes which includes the α-Gpdh locus.
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    An esterase duplication in Drosophila: Differences in expression of duplicate loci within and among related species (1982)
    Springer
    Biochemical genetics 20 (1982), S. 929-942 
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    ISSN: 1573-4927
    Keywords: esterase ; duplication ; gene expression ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract An esterase duplication is described in the sibling species pair Drosophila mojavensis and Drosophila arizonensis. We present evidence for two separate structural loci mapping at a distance of less than 0.16 recombination units from each other. Alleles at the two loci have the same substrate specificities and form small amounts of interlocus heterodimers. One locus (Est-5) is functioning throughout the insect's life cycle and appears at high concentrations in the hemolymph and the fat body. Its duplicate (Est-4) functions only during the late larval stage and is concentrated mainly in the carcass. No null alleles at either locus were observed in population surveys. An examination of 12 other species from the repleta group, to which D. mojavensis and D. arizonesis belong, suggests that Est-5 is universally present, but the activity levels of Est-4 vary among species and may be totally absent in some species. Variation in the level of Est-4 activity does not closely follow the phylogenetic relationship.
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    The association between malathion resistance and acetylcholinesterase in Drosophila melanogaster (1982)
    Springer
    Biochemical genetics 20 (1982), S. 179-198 
    Details
    ISSN: 1573-4927
    Keywords: acetylcholinesterase ; Drosophila ; malathion ; insecticide resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The relationship between the 50% survival time for flies feeding on a malathion-containing medium and the activity of acetylcholinesterase (AChE) was determined for 15 isofemale lines of Drosophila melanogaster. A significant correlation was found (r=0.28, P〈0.05), with more resistant lines tending to have a lower level of AChE activity. An association between AChE and malathion resistance was also observed in a selection experiment. The AChE activity decreased in two of two populations selected for malathion resistance. AChE from these populations was altered in kinetic parameters (measured in crude head extracts) and electrophoretic mobility. Although the “resistant” AChE had a lower activity (V m) on either a per milligram protein or a per individual basis, its apparent K m for acetylthiocholine was lower than that of “susceptible” AChE. Recombination mapping of both low activity and fast electrophoretic mobility localized these traits to the region of the structural locus (Ace) on the third chromosome. The AChE activity of flies heterozygous for a variety of Ace lesions (kindly provided by Dr. W. M. Gelbart) was consistent with this location. The changes in AChE were suggested to have been caused by selection of alleles at the Ace locus.
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    Variation in the biochemical properties of the Drosophila alcohol dehydrogenase allozymes (1984)
    Springer
    Biochemical genetics 22 (1984), S. 153-168 
    Details
    ISSN: 1573-4927
    Keywords: alcohol dehydrogenase ; Drosophila ; activity ratio ; specific activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Thirteen Drosophila Adh variants have been characterized with respect to gene expression, substrate preference, thermostability, and specific activity. The results suggest that the variants may be grouped into two biochemical classes, typified by the properties of the two most common enzyme forms, ADH-F and ADH-S. Membership of these classes cannot be predicted from electrophoretic mobility, nor is any simple classification possible with regard to the characteristics of level of gene expression (in terms of ADH activity or ADH protein) or thermostability of the gene product.
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    Acetaldehyde utilization and toxicity in Drosophila adults lacking alcohol dehydrogenase or aldehyde oxidase (1984)
    Springer
    Biochemical genetics 22 (1984), S. 1015-1029 
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    ISSN: 1573-4927
    Keywords: acetaldehyde ; alcohol dehydrogenase ; aldehyde oxidase ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Metabolic utilization and toxicity of acetaldehyde were studied in flies lacking alcohol dehydrogenase (ADH), aldehyde oxidase (AO), or both functions. Prior to the experiments, mutant alleles Adh n4 and mal were transferred to the same genetic background by 10 successive backcrosses. By comparison with wild-type flies, various deleterious, pleiotropic effects could be attributed to the mal allele but not to Adh n4 . Of the four genotypes studied (mal, Adh n4 , mal Adh n4 , and wild), all were able to use acetaldehyde as a resource in a similar way. In spite of its high toxicity, acetaldehyde appeared a better resource than ethanol. Flies treated with intermediate acetaldehyde concentrations (around 0.5%) exhibited a very high interindividual heterogeneity which could reflect a physiological adaptation occurring as a consequence of the aldehyde treatment. Toxicity tests showed that ADH-negative flies were more sensitive to acetaldehyde than wild type, but this is most likely explained by the transformation of the aldehyde into alcohol. Our results show that the aldehyde metabolizing enzyme (AME) system in Drosophila is neither ADH nor AO. The existence of an aldehyde dehydrogenase is plausible.
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    Genetic, ontogenetic, and tissue-specific variation of dipeptidases in Drosophila melanogaster (1982)
    Springer
    Biochemical genetics 20 (1982), S. 407-424 
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    ISSN: 1573-4927
    Keywords: dipeptidases ; Drosophila ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Three dipeptidases in Drosophila melanogaster are under independent genetic control and their structural genes have been localized, Dip-A to 2R and Dip-B and Dip-C to 3R (Voelker and Langley, 1978; Ohnishi and Voelker, 1981). These enzymes were characterized with respect to their substrate specificities, genetic variability (electrophoretic mobility and quantitative activity level), ontogeny (activity and isozyme pattern), and tissue localization. The dipeptide substrate specificities of DIP-A and DIP-B overlap each other considerably, but do not overlap with DIP-C. In natural populations, DIP-B and DIP-C are essentially monomorphic electrophoretically whereas DIP-A is polymorphic for three allozymes. Both DIP-A and DIP-B show quantitative genetic variation of activity level within an allozyme class. All three enzymes are expressed at all stages in the life cycle, but DIP-A and DIP-B activities vary considerably according to developmental stage and sex of adult. The tissue localizations of DIP-A and DIP-B activities show similar patterns and a nearly ubiquitous occurrence of both enzymes, but with particularly high values in larval and adult midguts and in the adult female reproductive system. These results suggest a general metabolic role for the enzymes, such as regulation of the concentrated pools of amino acids and oligopeptides found in Drosophila tissues.
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    Role of glandular scales of lepidote rhododendrons in insect resistance (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1787-1798 
    Details
    ISSN: 1573-1561
    Keywords: Coleoptera ; Curculionidae ; Otiorhynchus sulcatus (F.) ; black vine weevil ; Ericaceae ; Rhododendron ; trichomes ; glandular scales ; essential oils ; volatiles ; plant resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Glandular scales on selected lepidote rhododendron species varied in density from 109 ± 13 to 4180 ± 60/cm2 of leaf surface. Globules contained within the scales stained with Sudan IV, a lipophilic dye. Essential oil contents of the scales varied with species from 24 ± 8 to 151 ± 35 ng/scale. Black vine weevil [(Otiorhynchus sulcatus (F.)] feeding on leaves from a sample of rhododendron species was inversely related to leaf essential oil content, and weevil feeding on membrane filters was inhibited by application of essential oil extracts from leaves of most lepidote rhododendrons tested. Results suggest that the glandular scales of the lepidote rhododendrons function, at least in part, in plant defense against insects.
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    Attraction of bark beetles (Coleoptera: Scolytidae) to a pheromone trap (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 723-752 
    Details
    ISSN: 1573-1561
    Keywords: Ips typographus ; pheromone ; release ; recapture ; diffusion ; model ; Coleoptera ; Scolytidae ; trap ; marking ; dispersal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The movement of bark beetles near an attractive pheromone source is described in terms of mathematical models of the diffusion type. To test the models, two release experiments involving 47,000 marked spruce bark beetles [Ips typographus (L.)] were performed. The attractive source was a pheromone trap, surrounded by eight concentric rings with eight passive trap stations on each ring. Captures were recorded every 2–10 minutes for the pheromone trap and once for the passive traps. The models were fitted to the distribution in time of the central pheromone trap catch and to the spatial distribution of catch among the passive traps. The first model that gives a reasonable fit consists of two phases: Phase one—After release the beetles move according to a diffusion process with drift towards the pheromone trap. The strength of the drift is inversely proportional to the distance from the traps. Phase two—those beetles attracted to, but not caught by, the pheromone trap are no longer influenced by the pheromone, and their movement is described by a diffusion process without drift. In phase two we work with a loss of beetles, whereas the experiment seems to indicate that the loss of beetles in phase one is negligible. As a second model, the following modification of phase one is considered: After release the beetles move according to a diffusion process without drift, until they start responding to the pheromone (with constant probability per unit time), whereafter they start moving according to a diffusion process with drift. This study, like other release experiments, shows that the efficiency of the pheromone trap is rather low. What is specific for the present investigation is that we try to explain this low efficiency in terms of dynamic models for insect movement. Two factors seem to contribute: Some beetles do not respond to pheromone at all, and some beetles disappear again after having been close to the pheromone trap. It also seems that the motility of the beetles decreased after they ceased responding to the pheromone. Furthermore, the data lend some support to the hypothesis that flight exercise increases the response of the beetles to pheromone.
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    Receptor cells inIps typographus andDendroctonus micans specific to pheromones of the reciprocal genus (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 759-769 
    Details
    ISSN: 1573-1561
    Keywords: Ips typographus ; Dendroctonus micans ; Coleoptera ; Scolytidae ; exo-brevicomin ; (+)-ipsdienol ; single-cell recordings ; interspecific attraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Olfactory receptor cells were studied electrophysiologically inIps typographus andDendroctonus micans. The investigation revealed cells which were keyed to pheromone compounds characteristic of the reciprocal genus. Thus, cells keyed toexo-brevicomin were found inI. typographus, whereas cells keyed to (+)-ipsdienol were present inD. micans. Laboratory behavioral tests indicated an attractive effect of the two compounds on beetles of the reciprocal genus. InI. typographus the effect ofexo-brevicomin predominantly concerned males and enhanced their response to the pheromone “ipslure.” It is suggested thatexo-brevicomin serves as an interspecific attractant forI. typographus, which may be guided by pheromone compounds of the reciprocal genus in finding suitable breeding material. The function of (+)-ipsdienol inD. micans is more uncertain. It may be either a pheromone or an interspecific messenger.
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    Olfaction in the boll weevil,Anthonomus grandis Boh. (Coleoptera: Curculionidae): Electroantennogram studies (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1759-1785 
    Details
    ISSN: 1573-1561
    Keywords: Cotton boll weevil ; Anthonomus grandis ; Coleoptera ; Curculionidae ; pheromone ; kairomone ; plant odor ; olfaction ; electroantennogram ; attractant ; host plant ; green leaf volatiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Electroantennogram (EAG) techniques were utilized to measure the antennal olfactory responsiveness of adult boll weevils,Anthonomus grandis Boh. (Coleoptera: Curculionidae), to 38 odorants, including both insect and host plant (Gossypium hirsutum L.) volatiles. EAGs of both sexes were indicative of at least two receptor populations: one receptor population primarily responsive to pheromone components and related compounds, the other receptor population primarily responsive to plant odors. Similar responses to male aggregation pheromone components (i.e., compounds I, II, and III + IV) were obtained from both sexes, but females were slightly more sensitive to I. Both sexes were highly responsive to components of the “green leaf volatile complex,” especially the six-carbon saturated and monounsaturated primary alcohols. Heptanal was the most active aldehyde tested. More acceptors responded to oxygenated monoterpenes than to monoterpene hydrocarbons. β-Bisabolol, the major volatile of cotton, was the most active sesquiterpene. In general, males, which are responsible for host selection and pheromone production, were more sensitive to plant odors than were females. In fact, males were as sensitive to β-bisabolol and heptanal as to aggregation pheromone components. Electrophysiological data are discussed with regard to the role of insect and host plant volatiles in host selection and aggregation behavior of the boll weevil.
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    Quantitative variation of pheromone components in the spruce bark beetleIps typographus from different attack phases (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1029-1055 
    Details
    ISSN: 1573-1561
    Keywords: Ips typographus ; spruce bark beetle ; Coleoptera ; Scolytidae ; 2-methyl-3-buten-2-ol ; ipsenol ; cis-verbenol ; ipsdienol ; trans-verbenol ; verbenone ; myrtenol ; trans-myrtanol ; 2-phenylethanol ; ß-isophorone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Ips typographus beetles were collected in the field, separated into eight attack phases (from beetles walking on the trunk of a tree under attack to those excavating gallery systems with a mother gallery longer than 4 cm), and immediately frozen in liquid nitrogen. 2-Methyl-3-buten-2-ol,cis- andtrans-verbenol, verbenone, myrtenol, trans-myrtanol, ipsenol, ipsdienol, and 2-phenylethanol were quantified from excised hindguts against an internal standard, heptyl acetate, in the extraction solvent. Methylbutenol, the pinene alcohols, and 2-phenylethanol showed the same pattern of variation between attack phases in males, with the largest amounts present before accepting females and then a fast decline. Ipsenol and ipsdienol were not detected in males before the females were accepted, and the amounts increased when the females start their egg laying. Verbenone occurred only in trace amounts. The beetles were sampled from five Norway spruce trees (Picea abies) of differing resin flow. The correlations between the nine pheromone components and five major host monoterpenes in the gut showed that the variation in the amount of methyl-butenol, ipsenol, and ipsdienol could not be explained by the variation in the amounts of host monoterpenes. In contrast over 80% of the quantitative variation ofcis-verbenol,trans-verbenol, and myrtenol was explained by the amount of α-pinene. The nine pheromone components from 36 individual males were also quantified. Both methylbutenol andcis-verbenol showed a large variation in both amounts and proportions. Females containedtrans-verbenol and traces of most other components found in males. When accepted by the male, they also contained a female-specific compound, β-isophorone. Behavioral and biosynthetic implications of the results are discussed.
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    Electroantennogram responses ofScolytus multistriatus (Coleoptera: Scolytidae) to its pheromone components and to associated compounds (1982)
    Springer
    Journal of chemical ecology 8 (1982), S. 1333-1344 
    Details
    ISSN: 1573-1561
    Keywords: Coleoptera ; Scolytidae ; Scolytus multistriatus ; pheromone ; insect olfaction ; electroantennogram ; electrophysiology ; differential adaptation ; multistriatin ; 4-methyl-3-heptanol ; α-cubebene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Electroantennograms were recorded fromScolytus multistriatus in response to 4-methyl-3-heptanol, the four geometric isomers of multistriatin, and cubeb oil. Charateristic dose-response curves for response amplitude and the time required for the voltage trace to return to 1/2 baseline (recovery rate) were established. Recovery rates were significantly more rapid following stimulation with 4-methyl-3-heptanol or cubeb oil than with the multistriatin isomers. At most intensities, α-multistriatin, the isomer that evokes behavioral response, gave significantly larger EAGs with significantly longer recovery rates than the other isomers. Results of differential adaptation experiments suggested that 4-methyl-3-heptanol interacted with the processes involving multistriatin and cubeb oil activity. However, cross-activity of acceptors for these compounds seems unlikely; single sensillum recordings are needed to ascertain the response spectra for individual receptor neurons.
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    Frontalin in the male mountain pine beetle (1982)
    Springer
    Journal of chemical ecology 8 (1982), S. 1399-1409 
    Details
    ISSN: 1573-1561
    Keywords: Coleoptera ; Scolytidae ; Dendroctonus ponderosae ; pheromone ; frontalin ; exo-brevicomin ; Pinns contorta ; Pinus ponderosa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Frontalin andexo-brevicomin were identified by GC-MS in air drawn over maleDendroclonus ponderosae Hopk. (MPB) from Oregon that had joined females for 1–2 days in the bark of lodgepole and ponderosa pine logs. Unfed males releasedexo- andendo-brevicomin but not frontalin. These three compounds were not detected in either unfed or fed females. Arrestment of males bytrans-verbenol and terpenes in an olfactory walkway was reduced by the addition of racemic frontalin; production of attractant chirps also diminished. Racemic frontalin also strongly reduced the aggregation of MPB in lodgepole and ponderosa pine stands to sticky traps baited with the aggregation pheromonetrans-verbenol and host terpenes; however, the function of the natural enantiomer of frontalin in MPB is unknown.
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    Defensive secretions of three oxytelinae rove beetles (Coleoptera: Staphylinidae) (1982)
    Springer
    Journal of chemical ecology 8 (1982), S. 1411-1420 
    Details
    ISSN: 1573-1561
    Keywords: Coleoptera ; Staphylinidae ; Oxytelinae ; defensive secretion ; glands ; acetates ; lactones ; alkenes ; quinones ; citral
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The secretions of the abdominal glands ofBledius spectabilis Kraatz,Platystethus arenarius Fourcr., andOxytelus piceus L. have been shown to contain four 1-alkenes; toluquinone; toluhydroquinone; C10 −-,C11 −, C12 −-, and C14 − γ-lactones; C12-δ-lactone; citral; and decyl-, undecyl-, and dodecyl acetates. Quantitative results indicate that 1-alkenes are formed probably from present lactones by decarboxylation. According to the known life histories of the beetles, it is suggested that the gland material is not used as an algal growth regulator but represents a unique defensive blend characteristic for the whole subfamily.
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    Olfactory responses of adultTribolium castaneum (Herbst), to volatiles of wheat and millet kernels, milled fractions, and extracts (1982)
    Springer
    Journal of chemical ecology 8 (1982), S. 1463-1472 
    Details
    ISSN: 1573-1561
    Keywords: Red flour beetle ; Tribolium castaneum ; Coleoptera ; Tenebrionidae ; food volatiles ; olfactory responses ; olfactometer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Olfactory responses of 72- to 96-hr-old, 24-hr starved adult male, female, or mixed-sex groups of the red flour beetle,Tribolium castaneum (Herbst), to volatiles from wheat and millet kernels, certain milled fractions, and solvent extracts were recorded by using a lightsensitive apparatus. Wheat-germ volatiles from intact germ or solvent extracts were generally more attractive than volatiles of wheat endosperm or wheat bran. Volatiles of whole wheat kernels were the least attractive among test materials of wheat origin, whereas there were no significant differences among whole wheat flour, germ, and endosperm. Wheat-germ extracts, however, were more attractive than were extracts of the other fractions. Whole millet flour or fermented millet flour volatiles were more attractive than those from whole millet kernels or millet starch. Beetles reached maximum responsiveness to grain volatiles by 72–96 hr after adult ecdysis. Groups of virgin female beetles generally were more responsive than male or mixed-sex groups to volatiles of substances tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00989103
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  • 91
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    Influence of opposite sex on attraction produced by pioneer sex of four bark beetle species cohabiting pine in the Southern United States (1982)
    Springer
    Journal of chemical ecology 8 (1982), S. 373-378 
    Details
    ISSN: 1573-1561
    Keywords: Dendroctonus frontalis ; Ips calligraphus ; I. grandicollis ; I. avulsus ; Coleoptera ; Scolytidae ; mating ; attraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Catches ofDendroctonus frontalis andIps avulsus on traps surrounding bolts infested with both sexes of each species in the gallery were not significantly different from catches at bolts infested only with the sex which normally pioneers the host colonization process. In contrast, the presence of the second sex in the gallery significantly reduced catches ofI. grandicollis andI. calligraphus and, additionally, the presence of females in the male galleries ofI. calligraphus significantly reduced the catch ofI. avulsus females as compared with their response to bolts infested with the maleI. calligraphus only.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00987785
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  • 92
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    Defensive adaptations of eggs and adults ofGastrophysa cyanea (Coleoptera: Chrysomelidae) (1982)
    Springer
    Journal of chemical ecology 8 (1982), S. 453-462 
    Details
    ISSN: 1573-1561
    Keywords: Defensive secretions ; oleic acid ; hydrocarbons ; terpenoids ; eggs ; deterrents ; ants ; predation ; Chrysomelidae ; Coleoptera ; Gastrophysa cyanea ; reflex bleeding ; elytral glands
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Egg clusters and adults ofGastrophysa cyanea are conspicuous and, like their larvae, are chemically protected. The eggs owe their bright yellow color primarily to β-carotene and, in addition, contain substantial quantities of oleic acid. At natural concentrations oleic acid effectively deters many species of ants from feeding. The use of fatty acids as deterrents against ants is discussed as a possible widespread phenomenon among insects. During defensive confrontations, adults ofG. cyanea exhibit avoidance behavior and may also feign death. In addition, the adults may autohemmorhage or secrete a fluid from elytral or pronotal pores in response to traumatic stimuli. The secretions are effective against ants and contain a mixture of hydrocarbons as well as terpenoid components. The pattern of ontogenetic modification in the defensive chemical repertoire ofG. cyanea is discussed.
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    URL: http://dx.doi.org/10.1007/BF00987793
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  • 93
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    Responses of the olfactory receptors ofScolytus scolytus (F.) (Coleoptera: Scolytidae) to the stereoisomers of 4-methyl-3-heptanol (1982)
    Springer
    Journal of chemical ecology 8 (1982), S. 477-492 
    Details
    ISSN: 1573-1561
    Keywords: Scolytus scolytus ; Coleoptera ; Scolytidae ; pheromone ; 4-methyl-3-heptanol stereoisomer ; olfaction ; electrophysiology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Electroantennogram (EAG) and single-cell recording techniques have been used to demonstrate the presence of separate receptors for (−)-threo- and (−)-erythro-4-methyl-3-heptanol on the antenna ofS. scolytus. The majority of single-cell recordings showed spikes of two different amplitudes. The cell giving spikes of larger amplitude responded to the (−)-threo stereoisomer while the cell with the small-amplitude spikes responded to (−)-erythro-4-methyl-3-heptanol. It is suggested that in most recordings the two cells are associated with a single sensillum basiconicum.
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    URL: http://dx.doi.org/10.1007/BF00987796
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  • 94
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    Seasonal variation in laboratory response to behavioral chemicals of the southern pine beetle (1982)
    Springer
    Journal of chemical ecology 8 (1982), S. 641-652 
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    ISSN: 1573-1561
    Keywords: Dendroctonus frontalis ; Coleoptera ; Scolytidae ; seasonal variation ; pheromone content ; environmental parameters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The response ofDendroctonus frontalis to an attractant mixture (frontalin,trans-verbenol, and loblolly pine turpentine) was measured in the laboratory over a four-year period. Beetle response was highest in late winter and early spring, and lowest in midsummer and early fall. Males consistently responded higher than females. Female beetles displayed significantly higher responses in early morning and late afternoon than in the middle of the day. Analysis of beetle pronotal width and fat content revealed a high degree of correlation between these two parameters in female beetles, but there was no correlation of response with either fat content or pronotal width for either sex. There was no evident relationship between mean monthly beetle response and total amounts of frontalin andtrans-veibenol found in hindgut extracts. Daily temperature in months both during which beetles were bioassayed and immediately prior to bioassay was highly correlated to response to the attractant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00989633
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  • 95
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    Oscillatoria Sp. (Cyanophyta) mat metabolites implicated in habitat selection inBembidion obtusidens (Coleoptera: Carabidae) (1982)
    Springer
    Journal of chemical ecology 8 (1982), S. 671-678 
    Details
    ISSN: 1573-1561
    Keywords: Bembidion obtusidens ; Coleoptera ; Carabidae ; Oscillatoria animalis ; Oscillatoria subbrevis ; Cyanophyta ; metabolites ; volatiles ; ecophenes ; methylhexadecanoate ; methyloctadecenoate ; attractant ; habitat selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In laboratory bioassaysBembidion obtusidens Fall adults were attracted to volatile metabolites (methyl esters of hexadecanoic and 9-octadecenoic acid) of mat-forming, filamentous blue-green algae (Oscilalatoria sp.; Cyanophyta) growing in the microhabitat of these beetles on the shores of saline lakes. Commercial preparations of these metabolites also were attractive, suggesting thatOscillatoria metabolites are token stimuli which serve as habitat cues forBembidion adults.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00989636
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  • 96
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    Field bioassay ofexo- andendo-brevicomin withDendroctonus ponderosae in lodgepole pine (1982)
    Springer
    Journal of chemical ecology 8 (1982), S. 701-707 
    Details
    ISSN: 1573-1561
    Keywords: Coleoptera ; Scolytidae ; Dendroctonus ponderosae ; pheromone ; exo-brevicomin ; endo-brevicomin ; Pinus contorta
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Bothexo- andendo-brevicomin reduced the response of flyingDendroctonus ponderosae (Coleoptera: Scolytidae) to an attractant composed oftrans-verbenol and terpenes or oftrans- andcis-verbenol and terpenes in a stand ofPinus contorta var.murrayana. These data suggest that racemicexo- andendo-brevicomin may interrupt aggregation in populations of mountain pine beetle colonizing lodgepole pine; functions of the natural chiral compounds are unknown.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00988311
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  • 97
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    Correlated response to phototactic selection (1984)
    Springer
    Behavior genetics 14 (1984), S. 279-293 
    Details
    ISSN: 1573-3297
    Keywords: phototaxis ; Drosophila ; correlated response ; selection ; sepia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract Artificial selection for positive and negative phototaxis was conducted in populations ofDrosophila melanogaster that were polymorphic at thesepia locus. Photoselection response was accompanied by a correlated response in the frequency of thesepia allele. Changes insepia frequency were shown to be significantly different from those predicted by several neutral models. Implications of this correlated response are briefly discussed in terms of the neurogenetic basis of phototaxis.
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    URL: http://dx.doi.org/10.1007/BF01065547
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  • 98
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    A note on barometric pressure and behavior inDrosophila pseudoobscura (1984)
    Springer
    Behavior genetics 14 (1984), S. 315-317 
    Details
    ISSN: 1573-3297
    Keywords: Drosophila ; mating and barometric pressure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract During a study ofDrosophila mating behavior we observed unexpected changes in performance under ostensibly identical experimental conditions. We related the behavior during the 17 days of the experiment to changes in ambient humidity and barometric pressure. Humidity had no significant effect, but reduced barometric pressure was found to be associated with reduced mating activity (R 2=0.29,P〈0.025), accounting for close to 30% of the variation in total number of matings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01065549
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  • 99
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    Genetic elements of courtship inDrosophila: Mosaics and learning mutants (1984)
    Springer
    Behavior genetics 14 (1984), S. 383-410 
    Details
    ISSN: 1573-3297
    Keywords: Drosophila ; courtship ; learning ; circadian
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01065442
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  • 100
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    Genetic analysis of sex appeal inDrosophila (1984)
    Springer
    Behavior genetics 14 (1984), S. 411-440 
    Details
    ISSN: 1573-3297
    Keywords: Drosophila ; courtship ; pheromones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01065443
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