Summary
EMS induced temperature-sensitivelethal (2) giant larva, 1(2)gl, alleles were isolated by screening against a knownl(2)gl allele. Analysis of the lethal phase of thel(2)gl ts-deficiency heterozygotes demonstrated: (1) the majority of thel(2)gl tslarvae survive to late third instar, (2) at 29°C the majority of thel(2)gl tslarvae failed to pupate and only rarely did they differentiate adult cuticular structures, (3) at 15°C the majority of the larvae pupated and frequently differentiated adult cuticular structures. Examination of the imaginal discs ofl(2)gl tslarvae reared at 29°C revealed the presence of morphologically abnormal wing, haltere and leg imaginal discs. No morphologically abnormal discs were found in thel(2)gl tslarvae reared at 15°C. Studies on both the histology and the developmental capacity of the morphologically normal and abnormall(2)gl tsdiscs were performed. The morphologically normal discs are histologically normal and produce a full complement of adult cuticular structures. However, the morphologically abnormal discs contained both regions that maintained the normal monolayer epithelium and regions that had lost the normal tissue architecture. The implants obtained when the morphologically abnormal discs are injected into metamorphosing larvae contained only a limited number of the normal complement of adult structures and usually only structures found in the ventral wing hinge region were recovered. In addition, the “metamorphosed” morphologically abnormal discs contained undifferentiated tissue that gave rise to transplantable neoplasms when cultured in adults. The results of the studies on the pathology of thel(2)gl tslarvae are discussed with respect to the role of thel(2)gl tsfunction during normal development, the autonomy of the neoplastic development of thel(2)gl tstissues, and similarities between neoplastic development inDrosophila and mammals.
Similar content being viewed by others
References
Arking R (1975) Temperature-sensitive cell lethal mutants of Drosophila: Isolation and characterization. Genetics 80:519–537
Auerbach R, Auerbach W (1982) Regional differences in the growth of normal and neoplastic cells. Science 215:127–134
Bryant PJ (1975) Pattern formation in the imaginal wing disc ofDrosophila melanogaster: Fate map, regeneration and duplication. J Exp Zool 193:49–78
Ephrussi B, Beadle GW (1936) A technique of transplantation forDrosophila. Am Natur 70:218–225
Gateff E (1978a) Malignant neoplasms of genetic origin inDrosophila melanogaster. Science 200:1448–1459
Geteff E (1978b) Malignant and benign neoplasms ofDrosophila melanogaster. In: Ashburner M, Wright TRF (eds). The genetics and biology ofDrosophila, vol 2b. Academic Press, New York, pp 152–275
Gateff E, Schneiderman HA (1967) Developmental studies of a new mutation ofDrosophila melanogaster: lethal malignant brain tumor,l(2)gl 4,Am Zool 7:760
Gateff E, Schneiderman HA (1969) Neoplasms in mutant and cultured wild-type tissues ofDrosophila. Natl Cancer Inst Monogr 31:365–397
Gateff E, Schneiderman HA (1974) Developmental capacities of benign and malignant neoplasms ofDrosophila. Wilhelm Roux's Arch 176:23–65
Girton JR (1981) Patern triplications produced by a cell-lethal mutant inDrosophila melanogaster. Dev Biol 84:164–172
Girton JR, Russel MA (1980) A clonal analysis of pattern duplications in a temperature-sensitive cell-lethal mutation ofDrosophila melanogeaster. Dev Biol 77:1–21
Girton JR, Berns MW (1982) Pattern formation abnormalities induced inDrosophila by ultraviolet laser microbeams. Dev Biol 91:73–77
Hadorn E (1965) Problems of determination and transdetermination. Brookhaven Symp Biol 18:148–161
Hadorn E (1978) Transdetermination. In: Ashburner M, Wright TRF (eds). The genetics and biology of Drosophila, vol 2c. Academic Press, New York, pp 555–617
James AA, Bryant PJ (1982) Mutations causing pattern deficiencies and duplications in the imaginal wing disc ofDrosophila melanogaster. Dev Biol 85:39–54
Jürgens G, Gateff E (1979) Pattern specification in imaginal discs ofDrosophila. Developmental analysis of a temperature-sensitive mutant producing duplicated legs. Wilhelm Roux's Arch 186:1–25
Lewis EB, Bacher F (1968) A method for feeding ethylmethane sulfonate (EMS) toDrosophila males. Dros Info Serv 43:193
Lindsley DL, Grell EH (1968) Genetic variations ofDrosophila melanogaster. Carnegie Institute Publication No. 627
Russel MM (1974) Pattern formation in the imaginal discs of a temperature-sensitive cell-lethal mutant ofDrosophila melanogaster. Dev Biol 40:24–39
Schubiger G (1971) Regeneration, duplication and transdetermination in fragments of the leg discs ofDrosophila melanogaster. Dev Biol 26:277–295
Shearn A, Davis KT, Hersperger E (1978) Transdetermination ofDrosophila imaginal discs culturedin vitro. Dev Biol 65:536–540
Sonnenblick BP (1950) The early embryology ofDrosophila melanogaster. In: Demereced RM (ed). Biology ofDrosophila. Hafmer, New York, pp 62–274
Suzuki DT (1970) Temperature sensitive mutations inDrosophila melanogoster. Science 170:695–706
Williams JM (1981) Tumorigenesis inDrosophila melanogaster bearing the temperature-sensitive mutationshibere tsl.Dros Info Serv 56:158–161
Wright TRF, Steward R, Bentley DW, Adler PN (1981) The genetics of Dopa Decarboxylase inDrosophila melanogaster. III Temperature-sensitive DDC deficient mutation on female stemity. Dev Genet 2:233–235
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hanratty, W.P. Isolation and characterization of temperature-sensitivelethal (2) giant larva alleles. Wilhelm Roux' Archiv 193, 90–97 (1984). https://doi.org/10.1007/BF00848636
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00848636