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A novel method of DNA transfection by laser microbeam cell surgery (1984)

Tsukakoshi, M. ; Kurata, S. ; Nomiya, Y. ; [et al.]

Springer

Applied physics 35 (1984), S. 135-140

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ISSN: 1432-0649

Keywords: 07.60 ; 42.80 ; 87

Source: Springer Online Journal Archives 1860-2000

Topics: Physics

Notes: Abstract A new technique is presented to incorporate exogeneous gene materials (DNA) into cells with a microbeam irradiation from an uv pulsed laser. A frequency-multiplied Nd:YAG laser, 355 m wavelength, 5 ns pulse duration, punches a self-healing hole of submicrometer aperture in cell membrane under selected irradiation conditions. It takes a fraction of a second for the aperture to close, long enough to allow the foreign DNA, contained in the medium, to slip into the cell. The method offers a clear advantage over existing methods: increases the success rate of DNA transfection as well as the efficiency of cell modification by orders of magnitude.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00697702

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