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  • Biology  (200)
  • Temperature  (173)
  • Chemistry
  • LUNAR AND PLANETARY EXPLORATION
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  • Iranian Fisheries Science Research Institute  (371)
  • 1
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    Chrysiptera unimaculata Cuvier, 1830 (2021)
    Iranian Fisheries Science Research Institute
    In:  http://aquaticcommons.org/id/eprint/21041 | 17408 | 2017-04-27 05:58:40 | 21041 | Islamic Azad University, Science and Research Branch, Tehran
    Details
    Publication Date: 2021-07-13
    Description: Chrysiptera unimaculata, an algivorous species also living on the coral reef flat and being territorial but not considered as a strict farmer in this location. Maximum length is 8 cm in Persian Gulf. It is living in close association with macrophytes. Adults are found solitarily or in small groups among coastal algal reefs, rubble or over open beach-rock of reef flats exposed to moderate surge and feeds mainly on benthic algae. C. unimaculata is oviparous, distinct pairing during breeding. Eggs are demersal and adhere to the substrate. Males guard and aerate the eggs.
    Keywords: Biology ; Fisheries ; Chrysiptera unimaculata
    Repository Name: AquaDocs
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  • 2
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    Torpedo sinuspersici  Olfers, 1831 (2021)
    Iranian Fisheries Science Research Institute | Iran
    In:  http://aquaticcommons.org/id/eprint/21038 | 17408 | 2017-05-09 10:38:24 | 21038 | Islamic Azad University, Science and Research Branch, Tehran
    Details
    Publication Date: 2021-07-13
    Description: Electric rays, thought to be the most primitive of the skates and rays, have stout tails but have rather expansive disc. This group is distinguished by the presence of powerful electric organs, derived from branchial muscles in head region. Torpedo sinuspersici found inshore in sandy bottoms, and well offshore from the surf zone down to 200 m. Also on or near coral reefs (like Kish Island in Persian Gulf). Common in shallow sandy areas. Occasionally hooked by anglers, more often seen by divers; can deliver a strong shock. Flesh is edible. T. sinuspersici can survive for hours after being stranded on the beach. Little is known of the life history of the Gulf torpedo. It is a sluggish predator of bony fishes. At night it actively hunts for food, sculling slowly through the water about a meter above the bottom; during the day it usually rests on the bottom and opportunistically ambushes unwary prey. It uses its broad pectoral fins to envelop the target fish before delivering an electric shock to stun it. Usually solitary, they may form groups during the mating season. Reproduction is a placental viviparous, with the developing embryos initially surviving on their yolk sacs, and then on enriched uterine fluid produced by the mother. Litters of 9-22 young are birthed in the summer. Newborns measure about 10 cm wide; males mature at a disc width of 39 cm and females at 45 cm.
    Keywords: Biology ; Fisheries ; Torpedo sinuspersici
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  • 3
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    Boloceroides spp. (2021)
    Iranian Fisheries Science Research Institute
    In:  http://aquaticcommons.org/id/eprint/21044 | 17408 | 2017-04-27 06:00:37 | 21044 | Islamic Azad University, Science and Research Branch, Tehran
    Details
    Publication Date: 2021-07-13
    Description: Boloceroides spp. are looking like an untidy mop, this anemone is sometimes seen in sea grass areas on many of our shores. It is possibly seasonal. Sometimes, large numbers are seen (up to 10-20 animals in a trip) and then none at all.  Tiny swimming anemones may sometimes be confused with Sea grass anemones which have translucent tentacles with tiny spots. The swimming anemone harbors symbiotic single-celled algae (zooxanthellae). The algae undergo photosynthesis to produce food from sunlight. The food produced is shared with the sea anemone, which in return provides the algae with shelter and minerals. The oral disk and tentacle muscles are used to obtain, retain, and ingest prey; in Boloceroides spp. tentacles can autotomize if it is needed to evade a predator. Tentacles can control body form by use of their endodermal muscles. Retractors are longitudinal muscles that will aid in withdrawing tentacles and the oral disk if they are exposed to the open air.  This hypothesis is furthered because in comparison to other sea anemones, Boloceroides is loosely attached to its respective substrate, thus allowing the pedal disk to detach quickly resulting in a rapid swimming response. Boloceroides can reproduce both sexually and asexually. As Anthozoans, Boloceroides produce sexually by bypassing the medusa life cycle stage; this allows Boloceroides (and all Anthozoans) to release their egg and sperm creating planula a bilaterally symmetrical, flattened, ciliated, motile larva.
    Keywords: Biology ; Fisheries ; Boloceroides
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  • 4
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    Koumansetta hectori  Smith, 1957 (2021)
    Iranian Fisheries Science Research Institute
    In:  http://aquaticcommons.org/id/eprint/21042 | 17408 | 2017-04-27 03:56:54 | 21042 | Islamic Azad University, Science and Research Branch, Tehran
    Details
    Publication Date: 2021-07-13
    Description: Many aquarists know this fish as Ambylgobius hectori but accepted name is actually Koumansetta hectori. K. hectori, Hector's goby, is a species of goby native to the Indian Ocean (including the Red Sea), first record from Persian Gulf (Kish Island, Iran) to the islands of Micronesia in the western Pacific Ocean. It can be found on sheltered coral reefs at depths of from 3 to 30 meters (though usually between 5 to 20 meters). This species reaches a length of 8.5 centimeters (3.3 in) SL. This attractive little fish measures only 2” at maturity and spends its time hovering over rocks and substrates out in the open water column. Known for their bold yellow stripes, peaceful disposition, the diminutive Hector’s Goby is a nano reef favorite. It can also be found in the aquarium trade.
    Keywords: Biology ; Fisheries ; Koumansetta hectori
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  • 5
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    Pempheris vanicolensis (Cuvier, 1831) (2021)
    Iranian Fisheries Science Research Institute
    In:  http://aquaticcommons.org/id/eprint/21051 | 17408 | 2017-04-27 03:54:11 | 21051 | Islamic Azad University, Science and Research Branch, Tehran
    Details
    Publication Date: 2021-07-13
    Description: Pempheris vanicolensis has reported for the first time from Persian Gulf (Kish Island). Various aspects of the biology of P. vanicolensis Cuvier & Valenciennes, a recent Lessepsian (Suez Canal) immigrant into the Mediterranean, are given. Red Sea and Mediterranean populations were compared, and the results indicate that the spawning season is shortened in the Mediterranean, continuing from April to September, as opposed to year-round in the Red Sea. Descriptions of a ripe ovary and post-larva from the Mediterranean are given. Individuals reach a mean size of 10.8 cm in their first year, and 14.4 cm in the second year. Little change has occurred in the diet of the immigrant population, and both populations feed nocturnally, chiefly on larval and adult stages of planktonic crustaceans. Direct underwater observations on the diurnal behavior of the species show that the fish leave their daytime cave shelter at sunset, congregate at a nearby site and then migrate inshore and disperse into small groups to feed. Before dawn, they reassemble at the cave's entrance, and finally enter it at sunrise, after the school has built up. Observations on Persian Gulf sweepers show that the fish are segregated into size groups, ranging 15–18 cm adults at depths of 3 m.
    Keywords: Biology ; Fisheries ; Pempheris vanicolensis
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  • 6
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    Population genetic structure of silver pomfret, Pampus argenteus, in Persian Gulf and Oman Sea in Iran and Kuwait waters by using10 Microsatellite Markers (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25257 | 18721 | 2018-09-07 06:57:02 | 25257 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The silver pomfret, Pampus argenteus, is distributed in Indo-western pacific ocean, especially in Persian Gulf and Oman sea, and although is widely commercially exploited, the genetics structure of this species is not clear completely. In the present study, the genetic diversity and population structure of Pampus argentus in the Persian Gulf and Oman Sea were investigated using microsatellite DNA markers. Genomic DNA from 125 specimens was extracted and PCR amplification performed. Seven loci with reasonable polymorphism were amplified. The results showed that average of observed heterozygosity was ranged from 0.54 while expected heterozygosity was 0.67. After applying the Hardy-Weinberg Equilibrium (HWE) test, some loci were found to be significantly deviated from HWE in some populations in which deficiency of heterozygotes was apparent. Population differentiation observed among all populations (P〈0.01). The population differentiation (Fst) value between Kuwait and bushehr was the highest and significant (0.087) and between Chabahar and Bushehr (0.021) was the lowest and significant. Genetic distance analysis showed that the largest distance (0.269) was between Khuzestan and Chabahar and the smallest distance (0.075) was between Bushehr and Chabahar. The reported results could be of interest for management and conservation programmes of this species in the Persian Gulf and Oman Sea.
    Keywords: Biology ; Iran ; Persian Gulf ; Oman Sea ; Kuwait waters ; Bushehr Province ; Khuzestan province ; Chabahar ; Population ; Genetic ; Structure ; Silver pomfret ; Pampus argenteus ; Microsatellite markers ; Species ; Diversity ; DNA ; PCR ; Heterozygosity ; Equilibrium
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  • 7
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    An investigation of some biological aspects of three species: tiger toothed croaker, Otolithes ruber, Javelin grunter, Pomadasys kaakan and black pomfret, Parastromateus niger in the Oman Sea for optimizing fishing season (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25105 | 18721 | 2018-08-23 05:49:02 | 25105 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: During the years 1382-83, some biological aspects of three species Tiger-toothed Croaker, Otolithes ruber, Javelin grunter, Pomadasys kaakan, Black Pomfret, Parastromateus niger, have been investigated in the Oman Sea along Sistan-O-Baluchistan province. Based on the frequency of developed maturity stages (IV, V), a prologed spawning activity extending from August to March was determined for O.ruber, and the Gonadosomatic Index (GSI) suggests two peaks:the minor one in October and the major one in March-April. Comparison of Hepatosomatic Index (HI) and Condition factor (Kn) with the peak spawning indicated a reverse relationship. According to length at first maturity (Lm50%= 40cm) and exploitation of fish at length 42cm above, the stretched optimum mesh size of nets was calculated to be 100mm. On analysis of stomach food item, fish group dominated (80%), which of them Anchovy accounted for 40% of the total frequency. Comparison of mean length between the years 1374-75 and 1382-83 showed 4cm in decrees. Population dynamics parameters were determined through length based modals and the exploitation rate was estimated to be E=0.6, suggesting high level exploitation. The usal streched mesh size of nets(constructed from multifilament webbing) for capturing O.ruber were less than the proposed optimum mesh size of nets, indicating 78% of the total landings were smaller than Lm50%. Total landings in 1381 have increased up to 15% when compared to those of the year 1376. Despite the decrease of total efforts (Panel.day) by 37%, the mean value CPUE has increased up to 82%. Regarding Pomadasys kaakan species, a year-round spawning activity has been observed, with two peaks: A major one in April and an aminor one in September. As those of O.ruber species, there was an inverse relationship between HI and Kn with the peak spawning activity. Based on the length at first maturity (Lm50% = 44cm) and capturing fish of the length at 46cm and above, the stretched optimum mesh size of nets was calculated to be 149.6mm. According to analysis of stomach contents, fish group accounted for 35% of the groups studied, which of them , Largehead hairtail, Trichiurus lepturus linnaeus, constituted 52% of the total frequency. In addition to estimation of population dynamics, the exploitation rate was estimated to be E= 0.49. Majority of the species catch was made by mesh size of nets used for O.ruber of which 133mm mesh size accounted for the level (30%). Information on length distribution showed that 60% of the total fish landed was below Lm50%. Total landings in 1381 decreased to 10% in comparison to 1376.Total fishing efforts and CPUE also decreased to 33% and 24% respectively. For parastromateus Niger, There was a long period spawning activity from March to December, with two peaks: A major one in August and a minor one in March. According to Lm50% (39cm) and catching of fish 39cm above, the stretched optimum mesh size of nets was calculated as 167.7mm. Based on stomach contents, ring-shaped worms and larval shrimp equally made 29% of the total frequency showing the highest contribution. Comparison of mean length indicated 3cm in decrease in 1382-83 when compared to 1374-75. Population dynamics parameters were estimated and the exploitation rate was estimated to be E = 0.54. The usual stretched mesh size of nets for P. niger was 146mm and 81% of the total fish landed were smaller than Lm50%.In 1381,total catch of the species increased four and a half-fold in comparison to 1376, while total efforts decreased up to 28% and CPUE increased two and a half-fold.
    Keywords: Biology ; Fisheries ; Iran ; Oman Sea ; Investigation ; Biological ; Tiger toothed Croaker ; Otolithes ruber ; Javelin grunter ; Pomadasys kaakan ; Black pomfret ; Parastromateus niger ; Season ; Fishing
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  • 8
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    Survey on parasitic infections of Barbus spp. from south-west of Iran (Khouzestan Province) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25108 | 18721 | 2018-08-24 06:17:44 | 25108 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: A survey on parasitic infections on Barboid fishes of South- west of Iran (Khouzestan province) were done during Spring 2002 to Autumn 2004. Altogether 296 specimens from 5 different fish species were examined. The fishes were collected from five stations of Karoun River (Ahwaz-Goulestan, Molasani, Dez Dam), Karkheh River (Hamidia Dam) and Shadgan Lagoon. The fishes transported alive to the laboratory where they measured and weighted. Then they were killed by cutting the spinal cord. According to the results of this study 22 different parasites were revealed, 15 of then detected to the species level and 7 of them to the genus level. The metazoans are as follows: The monogenea, are Dactylogyrus onchoratus, D.persicus. Two digeneans: Diplostomum spathaceum, Asymphilodora sp. Five nematods: Rhabdochona denudata, Philometra karuensis, Philometra sp., Psudocapilaria tomentosa Contracecum larvae sp. and only one acanthocephalus, Neoechinorhynchus. Three protozoans are: Ichtyophthirius multifiliis, Trichodina sp., Goussia sp., Balantidium sp. And Myxozoans: Myxobolus persicus M. karuni M. nodulointestinalis, M iranicus, M.mesopotamia, M. pfief eri, Myxobolus sp., Myxidium rhodei, Myxidium pfiefferi. Histopathological investigation of some parasites was done as well. Pathologacal potential of M. nodulointestinalis and M. pfiefferi were revealed. According the results of this study three new parasites and eleven new hosts for Iranian freshwater fishes and three new host for three Myzoxoans were introduced.
    Keywords: Biology ; Ecology ; Fisheries ; Iran ; Khouzestan Province ; Barbus fishes ; Parasites ; Protozoa ; Metazoa ; Dactylogyrus onchoratus ; Rhabdochona denudata ; Philometra karuensis ; Species ; Fish
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  • 9
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    The effect of attractant on the growth factor and survival rate of Penaeus semisicatus (green Tiger) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25109 | 18721 | 2018-08-23 05:41:58 | 25109 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This project was carried out in 2000 to determine the effect of attractant diet on growth and survival rate of Penaeus semisulcatus in fifteen treatment each with three replication were applied for 45 days. The results indicate that 40I B, 40II and T foods had highest effect on mean weight which were 0.1556, 0.1492 and 0.1164 grams respectively. The yield data were compared with regression which showed us the positive correlation coefficient between yield and food consumption was 0.75.
    Keywords: Aquaculture ; Biology ; Iran ; Penaeus semisulcatus ; Food consumption ; Mean Weight and growth ; Survival rate ; Growth factors ; Green tiger
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  • 10
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    Determining nutritional requirements in Beluga (H.huso) from larval stage to marketable size (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25107 | 18721 | 2018-08-24 06:13:27 | 25107 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Growth experiments were designed and conducted in several phases in order to determine the most suitable starter diet, to determine the effects of different levels of protein and energy and also to determine the effects of different ratios of carbohydrate to fat on growth and body composition in farmed Huso huso from the larval stage up to the marketing stage. Phase one Growth experiments were conducted in a random statistical design to compare growth trends in H. huso larvae (mean weight 105.02 ± 0,02 mg) without being adapted to formulated diets. Experiments were conducted in fibreglass tanks (500 litre capacity) under similar culture conditions. Four replicates were run for each experiment. Larvae were fed four types of formulated diets (Diet A=100 % formulated diet produced in the International sturgeon Research Institute, Diet B=formulated diet imported from Holland, Diet C= formulated diet imported from France, Diet D= a mixture of Diet A + 10% gammarus) 8 times a day to satiation for a period of 49 days. The results obtained were compared with those obtained for the control group that was fed live food (Daphnia and Artemia). Mean water temperature during the experimental period was 18.52 ± 0.48 °C and mean dissolved oxygen was 7.35 ± 0.18 mg/l. The first signs of food granules were observed in the guts of the larvae after 12 h of the first feeding. All experimental groups were adapted to granulated diets on day 3 to day 5. In the beginning of the experimental period the larvae showed a no normal distribution (P 0.05) in terms of weight and length. However significant differences were observed in the end of the experimental period among the different groups studied in terms of growth, specific growth rate and food efficiency (P 0.05). Growth in group B and D were significantly higher than that in other groups except for the control group (P 0.05). It may thus be concluded that the olfactory organs of larvae took to Diet B (may be due to its composition) and to the diet D (because of its odour, texture and suitable flavour) more readily than other diets. At the end of the experimental period, maximum survival (98%) belonged to the control group. Survival rate in Group B and Group D was 90 % and 86.5%, respectively (P 0.05). Minimum survival belonged to fish fed Diet A (29.7%) and Diet C (45.5%). With regard to the adaptability of larvae to formulated diets, survival rates and growth and development in larvae we may conclude that the effects of different formulated diets on growth in larvae can be conducted on larvae with a mean body weight between 100 to 120 mg. In this weight class the digestive system and olfactory organ are fully developed and play a vital role in searching for food particles.
    Keywords: Aquaculture ; Biology ; Iran ; Huso huso ; Protein to energy ratio ; Carbohydrate to fat ratio ; Body composition ; SGR
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  • 11
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    The survey of feeding and reproduction of cetenophora Mnemiopsis leidyi in the southern of Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25112 | 18721 | 2018-08-23 05:48:23 | 25112 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Menmiopsis leidyi was entered into Caspian Sea from Black Sea and mass population constitutes in the southern Caspian Sea. In this surveys, the relationship between length and wet weight measured and length and weight followed by this equation: n=384, R^2=0.87 w=0.003L^9005 . The relationship of dry and wet weight calculated, in this surveys, the ratio of dry weight to wet weight was equal to 1.615% in 12 salinity. In vitro, the clearance rate at 12 and 27 C were 52.48 and 107.33 ml/h, respectively, daily ration at above temperatures were 1.98 and 16.65 respectively. Mnemiopsis leidyi can feed Acartia in accordance to 16.5 mg/1gbw (body weight) at 27 C in one day. The average digestive time in 12 C and 27 C were 77.50 min and 36 min respectively. The digestive value in 27 C was 2.5 fold than 12 C. The result indicated that clearance rate, daily ration and digest were increased in high temperature. Stomach content of Mnemiopsis leidyi was shown that this organism utilized from Acartia and Balanus, in addition, stomach content of kilka including two species of zooplankton from the southern Caspian Sea. Stomach contents of clupeonella cultiventris contain more than 54 percent of Acartia and its nauplii. This frequency observed in cluponella engrauliformis and clupeonella grimmi. On basis of zooplankton sampling in the sea, over 90 percentage of zooplankton populations contain Acartia.
    Keywords: Biology ; Iran ; Caspian Sea ; Menmiopsis leidyi ; Zooplankton ; Population ; Survey ; Feeding ; Cetenophora
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  • 12
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    Identification of native fishes of western Azarbaijan province - phase 1: northern water source (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25116 | 18721 | 2018-08-24 06:19:54 | 25116 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The aim of this research was to identify fish species distributed in the water resources located at West- Azarbaijan province. Sampling was done seasonally, from 14 sampling sites in main branches of different rivers including Aras, Zangmar, and Ghator. Fish samples caught with different tools, fixed in 10 % formalin, and finally transferred to the laboratory for biological investigations. The identified fish were 25 species belonging to 8 fish family comprising of Cyprinidae, Balitoridae, Cobitidae, Siluridae, percidae, poecilliidae, Gobiidae, Salmonidae. Among them, cyprinidae family had the highest percentage of diversity. There were 2 species from Balitoridae and 1 species from the rest of other family.
    Keywords: Biology ; Fisheries ; Iran ; Western Azarbaijan province ; Identification ; Native fishes ; Water
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  • 13
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    Study on propagation and breeding of Hamoun fish (shizothoraxzarudnyi) to one gram weight in earthen pond (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25322 | 18721 | 2018-09-12 09:50:04 | 25322 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Hamoun fish, Schizothorax zarudnyi, is an indigenous species of the eastern waters of Iran, which is exclusively found in this region. However recently drought occurrence in the Chahnimeh reservoirs (a semi natural water body) making them vulnerable to extinction. As an appropriate action to address this problem and according to the 3 side contract between Italian Government – UNDP - Islamic republic of Iran Government and the Italian government financial support to reduce poverty in the Province of Sistan-Baluchestan through the reinforcement and dissemination of aquaculture activities the project was developed at the Hamoun Research Unit by the Chahbahar Fisheries Research Center to record the breeding normative of 1 g weight larvae for restocking and other researching purposes. 331 broodstocks of the indigenous species Shizothorax zarudnyi weighing 800-2450 g were collected from the Chahnimeh reservoirs in early autumn, 2006. From 5/3/2007 (the project is supported and communicated on 2010) , Ovulation was stimulated with three stimulators; pituitary extract (3-6 mg kg-1 body weight), GnRH-A (20-30 mg kg-1 body weight) and anti dopamine (10-15 mg kg-1 body weight) that was given in 2-3 doses to breeders. Of 169 injected breeders , some were injected On mid March of 2007 (12-13 ºC water temperature) responded to the injection 25% ,while the rest were injected On April of 2008 (14-16 ºC water temperature) responded to the injection 65%. In the present project of 167 breeders 82 were female and 87 male. Totally 30 female breeders released their eggs in different stages. 20 female breeders released their eggs completely, 3 breeders released half of their eggs and 7 released 1/3 of their eggs. The male breeders just injected in the final dose of hormontrapy and all were ready for releasing sperms however the ovulation in female breeders occurs between 353-428 h ºC and after the final dose of injection. Ripe eggs were stripped from the females and fertilization was done by the dry method. Fertilized eggs were transferred to veis incubators and troughs. Incubation period for eggs differs and larvae hatch out after about 910 days at an average water temperature of 12.5 °C. Maintained at 13-14 °C, complete absorption of yolk sac in Shizothorax zarudnyi larvae occurred after 5-8 days. Larvae were fed with a mixture of powdered milk and egg yolk in this stage followed by decapsulated Artemia cysts and nauplii of Artemia and then on formulated starter diets used for carps. Because the ponds were not ready, larvae were maintained in troughs for about ten days before they were transferred to two 1200 m2 earthen ponds where they reached a body weight of about 1 g. They were then handed over to the Iranian Fisheries department in the region. Larvae were fed with the starter feed SFCO in the earthen ponds. About 350 thousand larvae were stocked in two earthen ponds. Based on the results of present study and other studies we may conclude that artificial breeding in Schizothorax can be successfully achieved at 14-16 ºC in flow through systems using hormone therapy (combination of GnRha and anti dopamine) and larvae could be easily cultured in earthen ponds. However this species exhibits lower growth rates as compared to carps its high expenses could have an important role in economical feasible.
    Keywords: Aquaculture ; Iran ; Sistan and Baluchestan province ; Chahnimeh reservoirs ; Chahbahar ; Hamoun fish ; Schizothorax zarudnyi ; Breeding ; Species ; Aquaculture ; Temperature ; Female ; Eggs ; Hormontrapy ; Sperm ; Ovulation ; Larvae ; Fisheries ; Earthen ponds
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  • 14
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    Investigation on feasibility polyculture of Litopenaeus vannamei with Mugil cephalus in earthen shrimp ponds (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25324 | 18721 | 2018-09-12 09:56:53 | 25324 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This study was conducted in order to optimizing of the biological condition and enhancing of the harvest efficiency for cultured shrimps of Guater site. Experimental design was consisted of three treatments and each treatment with three replications (totally 9 ponds with 600 m^2 area for each pond). After preparing and watering of ponds, the ponds was stocked with shrimp post larves in a density of 0.007±0.001 g (or 20 numbers per m^2) per m^2. After 35 days, the fingerlings of gray mullet were released to shrimp ponds in densities of 0/100 m^2 (T1/), 2/100 m^2 (T2) and 4/100 m^2 (T3). During experiment, the physicochemical parameters of water (temperature, O_2, pH, water transparency and salinity) were measured daily for two times (morning and afternoon). Also, the nitrate, nitrite, ammonia, phosphate and silicate concentrations and BOD5 as well as chlorophyll (a), phytoplanktons and zooplanktons were measured every 15 days one time. To determine the organic values of the bottom sediment of the ponds, monthly sampling was conducted. Health status of shrimps was investigated monthly too. Growth rate, mean weight, survival value, Food conversion ratios (FCR) and total shrimp harvest (Kg) were measured after 107 days rearing in ponds. ANOVA analysis showed significant differences between treatments. The highest (212 Kg, 3533 Kg; weight mean was18.4 g for each shrimp) and lowest (187 Kg, 3116 Kg; weight mean was16.23 g for each shrimp) of harvested shrimps were yielded in T3 and T1 respectively. There was not significant differences between treatments in terms of survival rate (P〉0.05). After 107 days rearing, the highest (1.27 0.2) and lowest (1.2 0.1) of FCR were observed in T_3 and T_1 respectively. Although, there were no significant differences between T_2 and T_3 in ammonia, O_2, Total organic material, pH and BOD5, but, T_2 and T_3 had significant differences with T_1 in these parameters. In this study, 27 genus of phytoplanktons belonged to Diatoms (Bacillariophyceae), blue-green algal (Cyanophyceae) and dinoflagellates (Dinophyceae) were identified. Diatoms with 20 genus had more abundance than dinoflagellates (6 genus) and blue-green algal (1 genus). Also, 7 orders of zooplanktons including copepod, mollusks, nauplius of crustacean and rhizopoda were identified. Health investigation of T1 (without shrimp) showed more pathogenic pollution (parasites and bacteria) than in treatments with shrimp. In this regard, among bacteria, the Vibrio genus (V.Alginoliticus and V.Fluvialis) had more abundance and among parasites zoothamnium sp and Epistylis sp were identified which more abundance was for zoothamnium sp. In conclusion, our results concluded that mixed culturing of white shrimp and grey mullet in optimum densities is possible and is caused more production of shrimp.
    Keywords: Aquaculture ; Iran ; Chabahar ; Guater ; Litopenaeus vannamei ; Mugil cephalus ; Mixed culturing ; Polyculture ; Earthen ponds ; Shrimp ; Fingerlings ; Gray mullet ; Temperature ; O2 ; pH ; Water transparency ; Salinity ; Chlorophyll a ; Phytoplanktons ; Zooplanktons ; ANOVA ; Diatoms ; Bacillariophyceae ; Blue-green algae ; Cyanophyceae ; Copepod ; Investigation
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  • 15
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    A survey on environmental effects of shrimp pond culture effluents in Gwatar area (Sistan & Blouchestan) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25327 | 18721 | 2018-09-12 09:54:28 | 25327 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Present project was conducted in shrimp farm located in east of Chabahar in Sistan and Balouchestan province.Twentieth sampling stations at three locations (i.e. 2 stations in supply channel,13 stations in drainage channel and 5 stations in Gwatar Gulf) were selected to determine physical and chemical factors such as; temperature, salinity, dissolved oxygen, pH, nitrate, nitrite, ammonia, phosphate and biological parameters for example Chlorophyll a, phytoplankton and zooplankton. Sampling was conducted once a month from March 2003 until January 2004. The results revealed different bioenvironmental area in the three locations (i.e. supply channel, drainage channel and Gwatar Gulf) over the study period. Concentration of salinity ,pH , nitatr ,nitrite ,ammonia and chlorophyll a in drainage channel were more than supply channel and Gawtar Gulf. While concentation dissolved oxygen and phosphate were in drainage channel less than Gawtar Gulf.Also, sediment with small grain size (clay and silt) caused uptake phosphate and amount phosphate decreased in drainage channel. Considering with lack of environment standards in Iran shrimp effluent, data compared with others countries standard. Results showed that with considerable farm area, value of temperature, salinity, dissolved oxygen and pH were in permit range in Gwatar Gulf while concentation nitrate, phosphate and chlorophyll a were sometimes more than permit range that could cause plankton bloom. Also, Gwatar Gulf were influence monsoon. Nitate, phosphate, chlorophyll a and phytoplankton increased in gwatar Gulf.Regards with that postmonsoon was simultaneus with shrimp harvest.Therefore, increase some parameters could be caused from shrimp effluent or postmonsoon. Thirty four phytoplankton genus were identified including diatoma, dinoflagellate, cyanophyta and chlorophyta. In each area maxiumum percentage of phytoplankton were belonged to diatoma (63%) in supply channel, cyanophyta (51.7%) in drainage channel and dinoflagellate (94.5%) in Gwatar Gulf.Amount and range chemical and physical factors and plankton composition were different in drainage channel, supply channel and Gwatar Gulf. Cyanophyta could grow more in drainage channel.Percentage of aboundance and diversity of phytoplankton changed in 2002 inrelation to years ago( before shrimp culture, 2000 year and 2001 year) specially in Gwatar Gulf. Mainly percentage of zooplankton were copepoda and thintinida respectively.Diversity zooplankton decreased ratio to years ago. In drainage channel, abundance total of phytoplankton and zooplankton were more than Gwatar Gulf and supply channel.
    Keywords: Aquaculture ; Iran ; Sistan and Blouchestan ; Gwatar ; Chabahar ; Environmental effect ; Shrimp culture ; Survey ; Sampling ; Temperature ; Salinity ; Dissolved oxygen ; pH ; Chlorophyll a ; Phytoplankton ; Zooplankton ; Diatoma ; Cyanophyta ; Chlorophyta ; Copepoda
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  • 16
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    A survey of some biological aspects of cobia (Rachycentron canadum) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25125 | 18721 | 2018-08-26 13:28:59 | 25125 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Biology aspects of cobia (Rachycentron canadum) were investigated from October 2005 to December 2006 in Northern waters of Persian Gulf (Hormozgan province).The reproduction cycle, sexual maturity, sex ratio, Fecundity, Lm50, feeding, length frequency, growth parameters and mortality of cobia were studied from total of 509 specimens ranged from 19 to 159 fork length. Gonadosomatic index peaked during spring and summer with main peak in June. Spontaneous spawning occurs around the year with peak in June. The overall female to male ratio was significantly 1 : 1.49 (P〈0.05). Batch fecundity were estimated 1684954±118990 in 15 females. Relationship between total length and fecundity were calculated F=1.3717TL 2.9567 (r^2= 0.82). Feeding studies indicated that the bony fishes were main food of cobia (76%) and followed by crustaceans (25%) and mollusks (11%). Rays were least food items (22%). Maximum and Minimum GaSI were observed in March (33%) and August (0.07%) respectively. Females reached 50% sexual maturity at 81.25 cm TL. The total length -weight relationship was W=0.0042L3.1162 (r^2=0.9852). Thetotal length-fork length relationship was TL= 1.1561FL-2.533 (r^2= 0.9933). Growth parameters K, L, and t_0 were calculated by von bertalanfy growth equation: 0.11 (year1), 168.65 cm, 3.49 and -0.97 respectively. The instantons rate of total mortality (z), natural mortality (M) and fishing mortality estimated by catch curve analysis were 0.30, 0.25, 0.14 (year1). Exploitation rate and Tmax were 0.36 and 28 year respectively.
    Keywords: Biology ; Iran ; Persian Gulf ; Hormozgan province ; Rachycentron canadum ; Cobia ; Sex ratio ; Fecundity ; Lm50 ; Feeding ; Mortality ; Growth ; Parameters ; Biology ; Maturity ; Spawning ; Fecundity
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  • 17
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    The monitoring of hydrology and hydrobiology stydies of the Persian Gulf in the Bushehr region (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25333 | 18721 | 2018-09-12 09:59:44 | 25333 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This study has been carried out in waters of Bushehr province in the Persian Gulf covering Nayband bay to Bahregansar between 26° 45 N to 30° 00 N and 49° 45 E to 52° 45 E, 15 main and 5 sub- stations, by R/V Ferdows 1 in winter 2004, summer 2004 and winter 2005. The physico - chemical parameters, nutrients (silicate, phosphate, nitrate and nitrite), phyto, zooplanktons, sediments and macrobenthoses were collected from water layers and sea bottom, and then analysed. The parameters were compared and evaluated with the results of the previous work, conducted in the same area. Termocline, halocline, oxycline and picnocline layers were formed in summer. These clins disappeared due to water column mixing in winter. The values of temperature, conductivity and pH in summer were higher than those of in winter, while the salinity and density values of surface and subsurface layers in winter were higher than those of in summer. The temperature and conductivity have been decreased from surface to depth, from coastal rigions to offshore and from southern waters to northwern waters, while salinity, density and turbidity have been increased. The dissolved oxygen is decreased by increasing the values of temperature, salinity and depth and it is observed that D.O. has increased in correlation with chlorophyl a in mean and subsurface layers. The pH was rather decreased from surface to depth while turbidity was increased. Orthosilicate (Si_SiO44), nitrate (N_NO3), and nitrite (N_NO2 ) concentration have increased from surface to depth while orthophostate (P_PO43) decreased. Among the phytoplanktons, Dinophycea had most abundance in winter 2004 and Diatoms had most abundance, variety and distribution in summer 2004 and winter 2005. Among the zooplanktons, Copeopda had most abundance, variety and distribution. The intensity of phytoplanktons in summer was more than those in winter; it decreased from surface to depth and from coastal rigions to offshore, at first increased and then gradually decreases. Intensity of zooplanktons in summer was more than those in winter and decreased from surface layers to depth and from coastal waters to offshore. The abundance of benthoses in summer was more than that in winter while their biomass in winter was more than that in summer. The abundance decreased from southern to northwern waters, but the rate of decrease in biomass was more than that of abundance. The mainly texture of sediments was loamy or muddy and the grain size in southeastern and northwestern areas were coarser than those in middle area. Compared with the previous study, the trend of physico chemical, silicate, nitrate and nitrite were the same but in spite of the past, the values of pH in summer were more than those in winter and also phosphate concentration had been decreased frome surface layer to depth. The overall trends of phyto and zooplanktons densities are almost similar to previous observation, but cyanophyceae significantly decreased, in comparison with spring and summer abundance. It is also observed that the zooplanktons average density has been in reverse order compared to previous observations. Average concentration of phosphate in the entire region was seven times more than that of the former study and its maximum value has rised more than 26 times in the petroleum and gas industries areas. On the other hand, the abundance and diversity of phyto and zooplanktons and also the ratio of biomass to abundance of benthoses were decreased.
    Keywords: Ecology ; Iran ; Persian Gulf ; Bushehr Region ; Nayband ; Bahregansar ; Hydrology ; Hydrobiology ; Nutrients ; Phytoplankton ; Zooplankton ; Sediments ; Macrobenthose ; Temperature ; Dissolved oxygen ; Chlorophyll a ; pH ; Diatoms ; Copeopda ; Depth ; Monitoring
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    Comparative survey of native species culture (Penaeus semisulcatus and Fenneropenaeus indicus) with non-native species (Litopenaeus vannamei) in Hele site of Bushehr province (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25339 | 18721 | 2018-09-14 06:59:46 | 25339 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The culture of three species green tiger prown (Penaeus semisulcatus) ,white leg shrimp (Litopenaeus vannamei) and Indian white shrimp (Fenneropenaeus indicus) were grown in 7 ponds (0.4 and 0.25 hectare) for 110 days at a stocking density of 25 shrimp/m2 on Hele Research Station. The average of temperature and salinity was 30 degrees centigrade and 46-48 ppt .At the end of the culture mean weights of L.vannamei 18.68±2.6 was higer than the P.semisucatus 7.77±1.49 and F.indicus 14.66 ±2.1 also results demonstrated that there was a significant relation (P〈0.05) between mean weight. Final yield were 3114, 1983 and 848 Kg for L.vannamei, F.indicus and P.semisucatus.The FCR was better for L.vannamei (1.28) than F.indicus (1.48) and P.semisucatus (2.15).
    Keywords: Aquaculture ; Iran ; L.vanname ; F.indicus ; P.semisucatus ; Mean Weight ; Culture ; Native species ; Green tiger prown ; Penaeus semisulcatus ; Shrimp ; Indian white shrimp ; Temperature ; Salinity ; FCR
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  • 19
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    Feasibility study on pomferet (Pampus argentus) breeding technique in Imam marine research station (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25342 | 18721 | 2018-09-13 12:35:36 | 25342 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This study was conducted during April to June 2007 .Pomferet brooders were captured by gill net (5 m Height, 300 m length, 7 cm size) during tidal time with 20-40 minutes interval. Khor Mermis, Khor Patil and Khor Mavi were subjected for broodstock captures. Totally 237 brooders were captured (126 male and 111 female) and were kept in 300 L. tanks. Semi dried method were used for breeding with 0.6 % fertility solution. Hatching were placed during 16-18 hoers. Fish larvae were kept in 4 T. tanks and were fed by fresh rotifer .temperature and salinity was kept 31-28 degree centigrade and 45-47 ppt. respectively .results shows successful breeding of pomferet in 2007 .egg diameter was 1.1 mm and hatching were completed during 16-18 hours at 31-29 degree centigrade. After 31 days length of larvae was 40 mm. while for 7 and 15 days length of larvae was 4.5 and 8.4 mm respectively.
    Keywords: Aquaculture ; Iran ; Feasibility ; Pomferet ; Pampus argentus ; Breeding ; Marine ; Gill net ; Height ; Length ; Size ; Broodstock ; Fertility ; Larvae ; Temperature ; Salinity
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  • 20
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    Feasibility study on semiintensive polyculture of chainess and Indian carps in I.R.IRAN (Guilan province) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25352 | 18721 | 2018-09-14 06:57:48 | 25352 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: 15000 juvenile Indian majour carps (Labeo Rohita,Cirhinus merigala, Catla catla) imported (200 ) and transferred to the earthen ponds in Astaneh Fisheries Research Station , In order to assess the viability of rearing under the climatic condition of north part of Iran and also achieve some growth parameters from the larval stage to market size.The fry with 300 mg weight, were released in to 12 ponds. Specimens were separated to tree experimental categories: 1= (30%indian carp and 70% chinees carp) , 2= (50%indian carp and 50% chinees carp) , 3= (70%indian carp and 30% chinees carp) .single chinees carp production were used as control . fishes were fed with artificial food and also with the natural products of the ponds which were enriched with organic fertilizers, after being equally stocked in the ponds .During the experiment, oxygen level ,PH, turbidity, and temperature were measured. Monthly fry samples were taken, for weight and length biometrical analysis and also determination of the weight gain percentage, average daily growth rate, specific growth rate. Results have indicated adaptations and considerable growth in this species, with an increase from the initial weight of 300 mg to an average of 13.5±1.6 mg in 50 days period until the end of November for fingerlings. Also the average weight of growing phase (second year of production ) was 647g for Labeo Rohita ,420 g. for Cirhinus merigala and 734g for Catla catla. The special growth rate (SGR)in Roho was 4.51 %.and 3.9 % for merigal and6.2 % for Catla while for Chinees carps SGR was 4.9, 6.7, 7.6 and 5.8 for silver carp,grass carp, common carp and big head respectively. Results revealed that the Indian majour carps compatibility with the condition in Gilan province was succesfull and the combination of species and optimization of their compatibility were remind for futhre studies.
    Keywords: Aquaculture ; Iran ; Guilan Province ; Astaneh ; Indian carp ; Growth ; Labeo Rohita ; Cirhinus merigala ; Catla catla ; Polyculture ; Juvenile ; Earthen ponds ; Fisheries ; Rearing ; Larval stage ; Fry ; Specimens ; Artificial food ; Fertilizers ; pH ; Turbidity ; Temperature
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  • 21
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    Assessment of different methods of water treatment on decreasing pathogenic bacteria in shrimp hatcheries (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25373 | 18721 | 2018-09-14 07:27:49 | 25373 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The project of "Assessment of different methods of water treatment on decreasing pathogenic bacteria in shrimp hatcheries" achieved by mean of reviewing the effectiveness of various methods on the water treatment to reduce bacterial load of sea water. The project performed in Bandargah region of Bushehr province in order to checking the effect of different methods on the sea water and bacterial population. This project has been done over a year. In this study a total of 138 samples was collected during seven times and was evaluated as well as 7 treatments. In this study, the comparison of the effect of each factors (sand filter, chlorine, ultra violet ray, chlorine combined with ultra violet ray) was analyzed on the sea water by means of disinfection. During this study, other environmental factors such as water temperature, dissolved oxygen, pH and salinity was measured till we can ensure that they don’t have any negative impact on the treatments. Results showed that the density of total bacteria in sea water (Control) was averagely 5187 CFU/ml, this was done while the total density of bacteria in the sea water, reservation pool sample, sand filter water, water that irradiated with ultraviolet rays, chlorinated water and the water that was influenced by both chlorine and ultra violet ray was respectively 5187 2137, 4449 2042, 2782 1335, 1164 692, 143 104, 87 76 CFU/ml. Therefore, reservation pool, sand filter, ultra violet ray, chlorine, chlorine-ultra violet ray each of them was reduce respectively 14, 46, 77, 97, 98 percent of the density of total bacteria or in other words they reduced 0.06, 0.27, 0.65, 1.56, 1.77 log of the total population of bacteria in water. Moreover, the results from the total count of vibrios showed that reservation pool, sand filter, ultra violet ray, chlorine, chlorine-ultra violet ray each of them was reduce respectively 17, 47, 74, 98, 99 percent of the density of total vibrios or in other words they reduced respectively 0.08, 0.27, 0.59, 1.66, and 1.95 log of the total population of vibrios in sea water. Also the bacteria that isolated from each treatment were identified with the use of biochemical methods that totally were includes a wide range of bacteria, the identified vibrios were: Vibrio alginolyticus, V. costicola, V. fischeri, V. fluvialis I, V. fluvialis II, V. harveyi, V. natriegens, V. nigripulchritudo, V. parahaemolyticus, V. plagius I, V. vulnificus, V. anguilarum I, V. campbellii, V. nereis, V. proteolyticus, V. splendidus, and Unknown V. spp. Other identified bacteria belonging to the following genus: Acinetobacter, Aeromonas, Alcaligenes, Bacillus, Borkholderia, Eschricia, Enteroacter, Flavobacterium, Micococcus, Proteus, Pseudomonas, Serratia, Staphaphylococcus, Streptococcus, and Unknown Bacteria spp. By comparing the results, we concluded that the best result was related to treatment that the sea water had been disinfected after the combined effects of chlorine and ultraviolet ray. It was determined that this process in compared with other treatments, had been reduced the total number of bacteria, the total number of Vibrios, the number of sucrose fermentative vibrios, and the number of non-sucrose fermentative vibrios respectively 98, 99, 98, 99 percent. In other words, the number of bacteria was reduced respectively 1.77, 1.94, 1.82, 2.23 log. So in this study, the applied method was introduced as the most effective way of disinfecting sea water. Meanwhile, none of pathogenic bacteria for shrimp including Vibrio harveyi was isolated by using this treatment. Comparing the results showed that the combined effects of chlorine and ultraviolet radiation is the most effective applied method for disinfecting sea water.
    Keywords: Aquaculture ; Iran ; Bushehr province ; Bandargah region ; Shrimp ; Water treatment ; Sea Dissolved oxygenwater ; Bacteria ; Hatchery ; Assessment ; Population ; Temperature
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  • 22
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    The study on possibility of fish soup powder production from different fish species in Sistan O blochestan Province, Chabahar (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25361 | 18721 | 2018-09-14 07:10:40 | 25361 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Fish soup powder is produced from three kinds of fish in Chabahar catched in Oman sea white flesh of many low value fish like threadfin bream, perches etc. can be used to prepare instant fish soup powder. This product has a high consumer acceptability in many countries in this research project we used 4 low value fishes with local name of Sarm, Chaman, Aroos and Talal belonging to families Carangidea, Lethrinidea, Drepanidae and Scombroidea. soup powder packed in laminated pouches and the shelf life of it is one year in ambient temperatures. The quality used to be good up to 6 month but after this time the quality decreases gradually. The experiment on fish soup showed that the percentage of protein was 32 percent, fat 11 percent and humidity 12 percent and ash 4 percent. The experiment conducted on fish soup powder was peroxide, total microbial count, organoleptic test for 6 month.
    Keywords: Fisheries ; Iran ; Oman sea ; Sistanblochestan province ; Chabahar ; Fish Soup Powder ; Species ; Fish ; Threadfin bream ; Carangidea ; Lethrinidea ; Drepanidae ; Scombroidea ; Temperature ; Protein ; Microbial count
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    Recognition of fishes in rivers and basin of Qom (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25143 | 18721 | 2018-08-26 16:50:02 | 25143 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This study was carried out on rivers, basins & qanat of Qom region during the 1376-1378and Fish samples were taken from 2 permanent rivers (Qomrood ,Ghara_chag)& from some seasonal ones (Tagharood, Zavarian, Vesva, Biraghan). Also local fishes was followed in 100 qanats in the region and Random fish sampling was done in permanent river extension out of province. This study aimed to recognize different fish species in the province water reservoir and estimating the fisheries potential in the province. For fish sampling nets such as mashk, salik, hook and hand tailored were used. Electroshoker was not used at all. Results of fish recognition showed that fish samples belong to 12 species & 4 family of "cyprinidae", "Balitoridae", "cyprinodontidae" and "poecilidae".The qanat fishes of the montain (kahak, Khagestan) and pastora area (Ghanavat,jafarabad) included by two families of "cyprinid", "Balitoridea".In some qanat which were connected to rivers more species of fish and anotherAquatic animal were observed & sampled.At the and map of geographical distribution of local fishes was planned.
    Keywords: Biology ; Iran ; Qom province ; Qomrood ; Ghara_chag ; Tagharood ; Fish ; Samples ; Species ; Fisheries ; Electroshoker ; Cyprinidae ; Balitoridae ; Cyprinodontidae ; Poecilidae ; Aquatic ; Recognation
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  • 24
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    Investigation of production possibility of monosex female and sterile fish in rainbow trout Oncorhynchus mykiss (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25147 | 18721 | 2018-08-26 16:54:41 | 25147 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This investigation carried out for the first time in Iran inorder to prodcution of monosex female and also sterilization in Rainbow trout. In this study, the eggs of general females were fertilized with the sperm of sex reversed male and so monosex female population was produced in second generation and sterilization carried out with oral administration of 17α methy 1 testosterone and immenrsion and oral administiration methods were used in embryonic stage and from commencing of acitve feeding of larvae, respectiverly. For sex reversal , 13 treatments were considered totally, that the most percentage of male (100%) was observedc in a treatment including of orally administration of 0.5 ppm hormone for 60 days after commencing active feeding (P〈0.001). In the other treamtnet, different percentages of sex ratio including male, female, intersex and sterility were observed. The offspring of genral eggs fertilization with the sperm of masculinized fish were 100% female, chisquare test was shown the treatment of orally administration of 30 ppm hormone for 120 days after commencing active feeding that had been considered for sterilization, was produced 90% sterile fish (P〈0.001) and was changed the sex ratio significancthy. Morphological changes of the gonads and sperm ducts in matured fish and also histological changes in the gonads of fish in the treamtints were considerable.
    Keywords: Biology ; Iran ; Monosex ; Female ; Male ; Sterilization ; Rainbow trout ; Eggs ; Fertilized ; Sperm ; Population ; Sex ; Fish ; Oncorhynchus mykiss ; Rainbow trout
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  • 25
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    Potential estimation and exploitation of large pelagics in the Persian Gulf and Oman Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25137 | 18721 | 2018-08-26 16:54:02 | 25137 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: In order to come up with the responsible fishing pattern, there was a need to identify some of the biological characteristics and population dynamic parameters of yellowfin tuna, skipjack and longtail tuna in the Persian Gulf and Oman Sea. Occurrence of empty stomach was high in all the specimens obtained from the gill net fisheries. Purple back flying squid was the most dominant prey species observed in the yellowfin tuna study (61% in males and 57% in females). The length of maturity (Lm50%) for yellowfin tuna was estimated 76 cm, 62.5 cm skipjack and 62.5 cm longtail tuna. Length range of skipjack was from 31-100 cm and bony fishes were the most dominant prey species observed in the skipjack. The monthly gonad somatic index was driven higher values after June which could be indicated as spawning period. The catch per unit of effort of three purse seiners (Azadegan 1, 2, 3) was compared in 1998- 2000. The species composition was 47% yellowfin tuna, 51% long tail tuna and 2% skipjack.
    Keywords: Biology ; Iran ; Persian Gulf ; Oman Sea ; Pelagics ; Biological characteristics ; Population ; Yellowfin tuna ; Skipjack ; Longtail tuna ; Specimens ; Gill Net ; Fisheries ; Purple back flying squid ; Species ; Maturity ; Length ; Gonad Somatic Index ; Spawning ; Estimation ; Potential
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    Surveying the biology of Capoeta capoeta gracilis and it s distribution, the goal aquaculturize the species (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25150 | 18721 | 2018-08-26 16:58:21 | 25150 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This Research has been done monthly in Sefidroud River and its tributaries from Agust 2003 to July 2004. From the total number of 2027 specimens of caught fish, 800 and 629 were males and females respectively, and 598 specimens were undistinguished. Samples were caught by cast nets. Fork length range of all specimen was 33-255 mm (119.56 ±46) and their weight rang was 0.42-257.5 gr (34.4 ±39.06) gr and their age range was 0-5 (1.76 ±0.959) year. The average of relative frequency in C.c.gracilis was 65.1 % . The average of condition factor was 1.07±0.13. Between Fork length and weight of C.c.gracilis. Was exponential relation and coefficient of correlation was estimated 99.11%. Growth cofficient (k) was 0.114 ; L_∞ was estimated 373.5 mm. C.c.g consumed of Chrysophyta phylum more than other Phytoplankton phyla in Sefidroud river and from Bentic point of view, consumed of clay, Filamentous algae detritus, insects, seeds. Absolute fecundity range was 1823-9274 (4553.8 ±2169.1) eggs. Considering the GSI and frequency percent of maturity stages, spawning period of the fish begins from April and peak of spawning is in June and terminates in July.
    Keywords: Biology ; Iran ; Sefidroud River ; Survey ; Capoeta capoeta gracilis ; Aquaculturize ; Species ; Specimens ; Males ; Females ; Fish ; Samples ; Weight ; C.c.gracilis ; Growth ; Chrysophyta phylum ; Phytoplankton phyla ; Filamentous algae detritus ; GSI ; Maturity ; Spawning
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    Study of diversity & abundance of ichthyoplankton in Sistan & Baluchestan Province (phase: Guatr Creek & Bay) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25151 | 18721 | 2018-08-26 16:53:58 | 25151 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Identification & determination of abundance Ichthyoplankton in Gouatr (Creek & Estuary) in Chahdahar region was carried out from Octber 2005 to September 2006. Sampling period was seasonal and 6 stations were selected. Sampling stations no. 1, 2 ,4 were located in the sea waters of Creek , no.5 & 6 in Gouatr Estuary and no.3 was near to Bahokalat Estuary. The results of environmental factors measurement were as follaws: Main depth of area: 4m, Salinity: 37.08 (g/l), pH: 8.19, air temperature: 29.8? and water temperature: 27.3?, Clarity: 0.75m Sampling was conducted using single ring Bongo-net with 333µ of mesh size. Totally, 585 specimens of diverse fish larvae stages were collected in 20 sampling. The dominant families of fish larvae were Gobiidae, Clupeidae and Engraulidae with 86% of total abundance and 11 families of fish larvae were identified. Most of the fish larvae were observed in winter season and some of them such as Clupeidae, Gobiidae, Blennidae Engraulididae and Sillaginidae were found in most season. The stage of the most samples was Preflexion (87%) therefore , the area is a nursery ground. Commercial fish larvae were carangidea, Sparidae, Clupeidae, Sillaginidae, Hemiramphidae, Engraulidae and Nemeptridae but they were only 37% from total abundance. The main diversity was 0. 6236. There is no correlation between abundance, temperature and salinity. The results showed a significant different between abundance from pre monsoon and post monsoon but no significant different between diversity in pre monsoon and post monsoon.
    Keywords: Biology ; Iran ; Guatr ; Sistan & Baluchestan ; Larvae ; Fish ; Identification ; Abundance ; Diversity ; Ichthyoplankton
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    Monitoring of Cochlodinium sp for shrimp farms in Hormozgan Province (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25423 | 18721 | 2018-09-23 16:29:10 | 25423 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The Blooming due to the some species of phytoplanktons especially Dynoflagellates has made some problems for water ecosystems and aquaculture. In this study, the density of phytoplanktons specially Cochlodinium sp. and also environmental factors such as temperature, pH, dissolved Oxygen, and transparency were recorded two weekly in 18 stations of Hormozgan province, Iran in order to monitoring of the possibility of phytoplankton blooming. During six months monitoring, the target phytoplankton, Cochlodinium sp was not observed in shrimp farms. But, other phytoplanktons and zooplanktons were observed as follow: 13 genus of phytoplankton and six genuses of zooplanktons has found in ponds, main water channel and sea. The diatoms with 10 genuses had the highest abundance and Dynoflagellates with 3 genuses had the lowest abundance and blue-green phytoplankton with one genus was in lowest group. Totally, diatoms with 77%, Dynoflagellate with 15% and blue-green alga with 8% abundance were the main populations of planktons in the studied area.
    Keywords: Aquaculture ; Iran ; Hormozgan Province ; Tiab ; Algae Cochlodinium sp ; Shrimp farms ; Species ; Phytoplanktons ; Dynoflagellates ; Aquaculture ; Temperature ; pH ; Dissolved oxygen ; Transparency ; Zooplanktons ; Blue-green algae
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    Studying native fishes in Hamadan province (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25437 | 18721 | 2018-09-23 16:57:02 | 25437 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Studying native fishes of Hamadan province have been done in 159 stations from 51 important water resources (wetland, reservoir, spring, river and qanat) using with electric tool, cast-net, seine and gill-net gears from July 2010 to Oct. 2011 and the main aims were species identifying and determining their distribution and abundance in the studied area. In the study, 33411 fish specimens are caught in 257 times of sampling and selected randomly about 8500 individual and laboratory works showed the fish belong to 37 species from 7 families. Cyprinidae with 25, Nemacheilidae with 6 and Sisoridae with 2 species had the most diversity and Cobitidae, Poeciliidae, Salmonidae and Mastacembelidae had only a representative. 31 fish species were native or endemic and 6 species were alien. Fish species existed in all rivers of Ghezelozan and Sirvan sub-basins but there were not any fish in 10 rivers of Ghara-Chai sub-basin and in 6 rivers of Gamasiab sub-basin, too. Also, it was observe 1-3 fish species in 15 rivers, 4-6 fish species in 10 rivers, 7-10 fish species in 5 rivers and more than 10 fish species in 4 main water resources and Gamasiab sub-basin with 32 fish species was the most diversified and Ghezelozan sub-basin with 2 fish species was the least diversified. There were any species to 4 (mostly 1 or 2) fish species in studied qanats in Ghara-Chai and Gamasiab rivers sub-basins. 23 fish species existed in a subbasin, 12 species in 2 sub-basin, Capoeta capoeta in 3 sub-basin and Squalius cephalus in all sub-basins of studied area. Alburnoides nicolausi,Capoeta aculeata and Alburnus mossulensis have had the most frequency. Studying fish abundance showed Oxynoemacheilus argyrogramma with 17.8%, Garra rufa with 12.3%, A. mossulensis with 12.1% and C. aculeata with 10.2 % of total number of caught fish specimens are dominant. S. cephalus, Capoeta damascina, C. aculeata, C. trutta, Chondrostoma regium and A. mossulensis have had sport fishing value but Acanthobrama marmid, Oxynoemacheilus kiabii, Oxynoemacheilus kermanshahensis, Turcinoemacheilus kosswigi, Alburnus caeruleus and Mastacembelus mastacembelus have biodiversity value for being endemic or having limited habitats in Iran.
    Keywords: Biology ; Iran ; Hamadan Province ; Gamasiab River ; Ghara-Chai ; Gaveh Rud ; Ghezelozan ; Fish diversity ; Abundance ; Distribution ; Specimens ; Sampling ; Cyprinidae ; Nemacheilidae ; Sisoridae ; Cobitidae ; Poeciliidae ; Salmonidae ; Mastacembelidae ; Capoeta capoeta
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    Determination of effective parameters on growth and bloom forming of Cochlodinium polykrikoides (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25436 | 18721 | 2018-09-23 16:56:01 | 25436 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Harmful algal blooms resulting in red discoloration of coastal waters in the Persian Gulf, Iran were first observed in January 2007. The species responsible for the bloom, which was identified as Cochlodinium polykrikoides, coincided with massive aquatic organisms’ mortalities in the Persian Gulf. In order to provide optimum growth and bloom forming, C. polykrikoides cells were sampled during the bloom conditions in the coastal waters of Persian Gulf. After adaptation in filtered seawater, they isolated by positive phototropism characteristic of this species to light. They were grown in modified media culture at different salinity (30, 32 and 35ppt), temperature (20, 23, 26 and 28ºC) and intensity (35, 70 and 90 µmol m^-2 s^-1) with an initial cell density of 50 cell mL^−1. The results of the present study clearly showed that the highest alga biomass was obtained following culture by using A^2 medium under the 32ppt salinity, 26°C temperature, and under a 11h light:13h dark photoperiod regime at a light intensity of 90 µmol m^-2 s^-1 provided by cool white fluorescent tubes. Mean cell density of C. polykrikoides in a 60 liter tank for ten days reached to 32 × 10^6 cell L^−1. Moreover, individual C. polykrikoides chain with 18 cells was observed for the first time in cultures. Based on the results from the present study, as mentioned above, providing suitable media culture and physical condition (light intensity and temperature), bloom forming of C. polykrikoides start from day 8 to 10 and will be continued until day 24 t0 28. In the other hand, C. polykrikoide cells immediately crashed and destroyed.
    Keywords: Ecology ; Iran ; Persian Gulf ; Harmful algal bloom ; Cochlodinium polykrikoides ; Isolation ; Optimum growth ; Environmental parameters ; Phototropism characteristic ; Species ; Density ; C. polykrikoides ; Temperature ; Growth ; Biomass ; Bloom
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    The study on fishing and resource management of bony fisheries within southern Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25444 | 18721 | 2018-09-23 17:01:05 | 25444 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The project of the Study on fishing and resource management of fisheries within Southern Caspian Sea was conducted on the base of an agreement made between fisheries organization (Shilat) and the Iranian fishery research organization (IFRO) signed in 2010. In this library-based study the current situation of bony fish fisheries and biology of these species has been surveyed in Iranian waters of the Caspian Sea . In this survey internal and external environmental factors of bony fishes activities were considered. Using SWOT analysis method, current situation of fisheries was investigated..One of the most important strategies for conserving anadromous species such as Caspian kutum , common carp, roach, bream and …. is rehabilitation of the natural spawning ground in rivers, coastal lagoon specially Anzali lagoon .By conducting this strategy not only restocks of bony fishes is done ,but also reduced the cost of artificial restocking programs and releasing fingerlings .In short term , it is nessesary to enhance the stocks of the species that have low catch is done by huge releasing of the fingerlings and study the qualification of the program.
    Keywords: Ecology ; Management ; Iran ; Caspian Sea ; Anzali lagoon ; Bony fishes ; Distribution ; Stocks ; Biology ; Fishing ; Species ; Caspian kutum ; Common carp ; Roach ; Bream ; Spawning ; Fingerlings
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    Identification and extraction of streptococcusis gene from streptococcus iniae and recording in Gene Bank (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25458 | 18721 | 2018-09-26 09:47:40 | 25458 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: S. iniae is an important pathogen in both marin animals and humans, causing systemic infections in these hosts. The symptoms of infections are very similar to symptoms of infections caused by human¬specific streptococcal pathogens. The enzyme phosphoglucomutase (PGM) has recently been discovered to play an important role in polysaccharide capsule production and virulence in s. iniae. We aim to isolate S. iniae and cloning phosphoglucomutase gene. S. iniae grew on sheep blood agar as mucoid and beta- hemolytic colonies after 24 h of incubation at 37C. Then confirmed it by sensitive and rapid method of PCR. The pgm gene was amplified successfully and cloned in pTZ57R cloning vector.The recombinant plasmid was sub cloned into pETDuet-l expression vector by rectriction enzymes and confirmed by PCR. Although S. iniae is a pathogen of economic importance, relatively little is known regarding mechanisms of its pathogenesis and few specific virulence determinants have been established. These include the enzyme phosphoglucomutase which contributes to cell wall integrity and resistance to antimicrobial peptides. Thus, this enzyme is the best choice to product the vaccine and more investigations.
    Keywords: Biology ; Iran ; Streptococcusis ; Gene ; Streptococcus iniae ; Gene Bank ; S. iniae ; Pathogen ; Enzyme ; Phosphoglucomutase ; PCR
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    Study of some biological characteristics (Tiger tooth croacher, Silver pomfert, Yellow fin seabream, Hilsa shad, Indo-pacific king mackerel, Narrow-bared Spanish mackerel) in the coastal waters of the Persian Gulf (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25170 | 18721 | 2018-09-03 14:16:38 | 25170 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The present study was carried out in the fishing landing center in khozestan using the commercial catch data. The data of total cutch of fishes and effort were prepared from the deputy of statistic office. Based on the pre data, the effort of gill-net fishery was being decreased from 1996 to 2000 and then had a trend to increase from 2001. The maximum and minimum of trap fishery of dhows ware observed 2005 and 1996 respectively. The maximum and minimum of total catch were observed in 2005 in Hilsha shad and Spanish mackerel with 4174.95 tones and 73.33 tons respectively. In this year the total catch of silvery pomfret and tiger tooth croaher species were recorded 323.6 tones and 1700.44 tons respectively. The growth parameters of Hilsha shad were estimated from 10071 specimens in Abadan and 4032 specimens of length frequencies from Hendijan, statistic program of t-test was used for the mean total length and the results showed the significantly different (p 〈 0.05) in the two areas during 12 months. The Von Bertalanffy growth parameters were estimated from the FISAT II and ELEFAN.The asymptotic length (L) was estimated using the Powell – Vetheral method in Abadan and Hendijan, 42.74 cm and 37.02 cm respectively. Growth coefficient or K for both areas were 0.77 and 0.71 per year respectively. The instantaneous rotes of total mortalities (Z) were estimated from length-converted catch curves and the values were at 2.55 year^-1 for Abadan and 2.81 year^-1 for Hendijan. The instantaneous rate of natural mortalities (M) and fishing mortalities (F) for both areas, Abadan and Hendijan were 0.75 year^-1, 0.732 year^-1 and 1.8 year^-1, 2.078 year^-1 respectively. The exploitation rates (E) for both populations were 0.7 year^-1 in Abadan and 0.73 year^-1 in Hendijan. However the optimum level of E should be 0.5 and the estimated valves of E is more than 0.5 and shows the pressure of fishing effort and overexploited on the population of this stock. The instantaneous rate of total mortalities of Tiger tooth croacker was 1.95 year^-1 the instantaneous rate of natural mortality using Pauly empirical formula was estimated 0.7 year^-1 and fishing mortality were 1.52 year^-1. The exploitation rate for this species was calculated 0.62 year^-1. The values of M and F for silvery pumfret were obtained 0.91 year^-1 and 1.47 year^-1 respectively. The exploitation rate was estimated 0.61 year^-1.
    Description: ;
    Keywords: Biology ; Iran ; Persian Gulf ; Khozestan Province ; Abadan ; Hendijan ; Biological characteristic ; Tiger tooth croacher ; Silver pomfert ; Yelow fin seabream ; Hilsa shad ; Indo-pacific king mackerel ; Narrow-bared Spanish mackerel ; Fishing ; Gill net ; Fishery ; Specimens ; Mortalities ; Coastal waters
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    Comprehensive study on assessment of sturgeons population genetic structure in the Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25174 | 18721 | 2018-09-03 17:15:43 | 25174 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The population genetic structure of five Caspian Sea sturgeon species was investigated. Totally 1121 samples of caudal and unault's fin tissue of the sturgeons (Acipenser persicus, A. gueldenstaedtii, A. stellatus, A. nudiventris and Huso huso) were collected from the Volga River (Russia), Ural River (Kazakhstan), Kura River (Azerbaijan), Sepidrud River and the coastline of the south Caspian in the Iranian waters as well as from the sampling stations selected for the marine survey for sturgeon stock assessment in the Caspian Sea. All samples were stored in 96% ethyl alcohol and transferred to the genetic laboratory of the International Sturgeon Research Institute. Genomic DNA was extracted using phenol-chloroform method. The quality and quantity of DNA was assessed by Agarose gel (1%) electrophoresis and spectrophotometry. The population genetic structure of Ship and Persian sturgeon was studied using both PCR-RFLP (D-loop and ND5/6 gene) and microsatellite technique and that of H. huso, A. stellatus and A. persicus were studied using microsatellite technique. After amplification of genes using PCR, the RFLP technique was used to digest mtDNA using restriction enzyme. The PCR products were electrophoresed on 6% sequencing polyacrylamide gels followed by silver nitrate staining. Data for PCR-RFLP were analyzed using REAP program and those from microsatellite technique were analyzed using Gene Alex. Population genetic parameters including allele frequency, expected and observed heterozygosity, effective allele, Shannon's index were determined. Genetic identity and distance were calculated following Nei criteria and Hardy Weinberg equilibrium was tested based on X2 and analysis of molecular variance (AMOVA) using Reap and Gen Alex at 99% confidence limit. Phylogenetic relationship was determined and drawn using TFPGA program. The population genetic structure and genetic diversity of the 1121 sturgeon specimens were determined. Three independent populations were identified for Acipenser persicus (two populations in the south Caspian in the Iranian waters and one in the north Caspian). Three independent populations were identified for A. gueldenstaedtii (Volga, Ural and South Caspian populations) using the microsatellite technique. Population genetic structure using PCR-RFLP revealed no genetic differentiation among the A. gueldenstaedtii specimens studied from the different regions using ND5/6 gene, while two populations (Ural and south Caspian populations) were detected for this species with the same technique using D-loop genes. Four independent populations (Volga, Ural, Kura and Sepidrud populations) were reported for A. stellatus using the microsatellite technique and four more populations which most probably belong to the autumn and spring races of the above mentioned independent populations were identified for this species. The present study also identified two populations for H. huso; The North Caspian population (in Volga and Ural Rivers) and The South Caspian population (in Golestan and Guilan regions) which were significantly different from each other (P〈0.01). The genetic population structure of A. nudiventris was studied using the microsatellite and PCR-RFLP techniques which revealed two populations for this species one in the Ural River and the other in the Sepidrud River (South Caspian). Comparison of the ND5/6 and D-loop genes studies in Russian sturgeon revealed that the D-loop gene is better than the ND5/6 genes in population's differentiation and is therefore strongly recommended for population genetic studies on sturgeons in the Caspian Sea. Genetic diversity studied using microsatellite technique was higher and more accurate as compared to that using RFLP. Nevertheless the RFLP technique was able to introduce molecular markers for the population’s species pacific identification. On developing suitable primers these studies can be speeded up and the cost of such studies can be cut down. However the drawback in using microsatellite technique for population genetic studies is that it cannot introduce a molecular marker for the identification of populations. The present study was able to introduce molecular markers to differentiate the ship sturgeon population in the south Caspian from that in the Ural River using the PCR-RFLP technique Based on the results obtained it is strongly recommended that all activities related to restocking and rehabilitation of sturgeon stocks in Iran be conducted on the basis of genetic principles. Also serious and immediate measures should be taken for the restoration and conservation of rare population of native species of Iran particularly in the Sepidrud region using genetic markers before they are become extinct.
    Keywords: Biology ; Iran ; Caspian Sea ; Guilan Province ; Golestan province ; Sepidrud River ; Assessment ; Sturgeons ; Population ; Genetic ; PCR-RFLP ; Microsatellite ; Species ; Samples ; Tissue ; Acipenser persicus ; A. gueldenstaedtii ; A. stellatus ; A. nudiventris ; Huso huso ; Survey
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    Study on effects of Beta-glucan & extraction of Sargassum & Padina algae in prevention and treatment of WSD in Fenneropenaeus indicus (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25179 | 18721 | 2018-09-03 17:23:17 | 25179 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: In this study two species of algae, Sargassum glaucescens and Padina boergeseni that found plenteously in Persian Gulf and Bushehr coast, were collected and hot water extracts of them were lyophilized. F. indicus (11.32±1.20 g), after two weeks adaptation in Shoghab research station were immersed in seawater (39 ppt and 25±1 ○C) containing hot-water extract of each brown algae, S.glaucescens and P.boergeseni, at 100, 300 and 500 mg/l concentration, Survival rate and immunological parameters (total haemocyte count (THC), total plasma protein (TPP), Phagocytic activity, bacterial clearance efficiency and bactericidal activity) were examined. In addition effect of dietary administration of beta 1, 3 1, 6 glucan on prevention of White Spot Disease and immunological parameters of shrimp were investigated. According to results, immersion in seawater containing 300 and 500 mg/l concentration of algal hot-water extract after 2 and 3 hours or oral administration of beta 1,3 1,6 glucan at level of 10 g/kg diet for 14 days significantly enhanced THC, TPP, Phagocytic activity, bacterial clearance efficiency and bactericidal activity. Immersion in seawater containing 100, 300 and 500 mg/l hot-water extract of S.glaucescens after 3 hours, improved the survival rate of WSSV-infected F. indicus.
    Keywords: Biology ; Chemistry ; Iran ; Persian Gulf ; Bushehr Province ; Beta-glucan ; Sargassum ; Padina algae ; WSD ; Fenneropenaeus indicus ; Species ; Algae ; Brown Algae ; S.glaucescens ; P.boergeseni ; Survival rate ; White Spot Disease ; Shrimp
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    Preliminary cultivation of silver bream (Sparidentexhasta) in earthen pond using available artificial fish feed in Chowibdeh site-Abadan southern Iran (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25183 | 18721 | 2018-09-03 17:12:18 | 25183 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Possibility of rearing silver bream (Sparidentex hasta) in the earthen pond was experimented using 2 types of available artificial trout feed (FFT and GFT1). The experiment was carried out in 2 phases in Shahid-Kiani Extension Shrimp Farm in Chowibdeh Shrimp Site-Abadan Southern Iran, and earthen ponds with 0.25 ha. Area were used for both phases of the study. Juvenile fish of 3.2 g initial weight with the density of 9324 ind./ha. Were stocked for 196 days in the first phase. The initial weight, stocking density and rearing period of the fish in the second phase were 108.60 g, 9800 ind./ha. And 237 days respectively. Maximum amount of WG, FCR, PER and SVR of the fish in the first phase of the study were 100.12 g, more than 6, 0.38 and 55.32% respectively. WG and SR of the fish in the second phase respectively were 417 g and 20.74%. The Salinity of pond water was in the range between 9 and 22 ppt, and the water temperature was between 8.3 ֯C (mid December morning) and 34.8 ֯C (early Sep.....
    Keywords: Aquaculture ; Iran ; Abadan ; Chowibdeh ; Cultuation ; Silver Bream ; Sparidentexhasta ; Earthen Pond ; Artificial feed ; Rearing ; Shrimp ; Juvenile ; Weight ; Density ; Temperature
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    Primary fish cell culture phase 1: primary cell culture of the interior pituitary gland of the Acipenser gueldenstaedtii (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25177 | 18721 | 2018-09-03 17:18:50 | 25177 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Fish cell lines have been used to support many areas of research, beginning with fish viruses and extending into immunology, genetic studies, toxicology, environmental effects, aquaculture and seafood quality and it is the first step in the gene banking, to preserve gene materials. The present study we cultured cell of interior pituitary of the Acipenser gueldenstaedtii gland, as a first attempts in IRAN. FISH cell culture has widespread applications in virology, toxicology and as in vitro models in cytogenetic, biomedical, physiological researches. A cell line has been established from the Acipenser gueldenstaedtii interior pituitary and scales have been used to develop primary cell cultures. Recently, successful primary culture of the interior pituitary gland of Acipenser gueldenstaedtii has been developed by explants method. The present study evaluated the potential of several interior pituitary gland from different developmental stages for development of cell cultures using explants method. Pituitary gland from various stages of the adult Acipenser gueldenstaedtii was collected under standard aseptic conditions. Developing gonads from 15-20-year-old male and female sturgeon were collected during late April and early May2007. In all the cases the tissues were pooled in cold PBS antibiotic antimycotic solution (Sigma Chemicals, USA). The tissues were evaluated for attachment, growth and ability to undergo to produce suspension of cells. Primary cultures were initiated from the above tissues according to our earlier procedures, with certain modifications in the sub cultivation procedure. Briefly, tissues were cut into 1 mm3 size fragments which were seeded into 25 cm2 tissue culture flasks. After appropriate semidrying and addition of minimum essential medium (MEM) (Sigma, USA) supplemented with 15% fetal bovine serum (FBS) (Sigma, USA), cell growth was monitored. A seeding density of 1.5 105 cells as determined by a hemocytometer. The results have been showed the Acipenser gueldenstaedtii interior pituitary cells growth in incubator Co2 in 370 C, the cells adapted in this temperature. They were in Lag phase for 10 days, in log phase on 10- 22 days, and in stationary phase on 23- 28 days, after that they died. So we could produce sturgeon growth hormone from fish pituitary cells culture. By this study we can passage the cells on 21th day, for every week. In this way we can produce continued cell culture and store them for gene banking.
    Keywords: Biology ; Iran ; Acipenser gueldenstaedtii ; Pituitary gland ; Primary cell culture ; Fish
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    Identification of genetic marker for differentiation of Persian sturgeon (Acipenser persicus) from Russian sturgeon (A. gueldeustadtti) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25519 | 18721 | 2018-10-05 15:40:50 | 25519 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The Persian sturgeon (Acipenser persicus) is more abundant sturgeon species in the South Caspian Sea and consist the highest proportion of Iranian Caviar, meat as well as bringing maximum foreign currency income, however from systematic point of view and differentiation of this species from Russian sturgeon (Acipenser gueldenstadttii) a serious challenging issues remain, where some Russian scientist are believe that the Persian sturgeon is not as an valid species and consider it as a subspecies of Russian sturgeon. This research conducted with the objective of identification and introducing a molecular marker based on specific DNA for differentiation of two species of Persian sturgeon and Russian sturgeon via a proved molecular marker method. For this purposes 8 different molecular approaches such: Microsatellite, AFLP, RAPD, sequencing of Cytb, 16sDNA, ND5, Growth Hormone gene and finally Single Nucleotide Polymorphism (SNP) were investigated. Based on applied methodology, between 5 to 16 caudal fin tissues were sampled for each species from different region of the Caspian Sea, Sefiedrud River, Ural and Volga rivers. Following DNA extraction, its quality and quantity were determined and the PCR experiment has been conducted using 5-110 primers according to various methods and type of gene. The PCR products were electrophoresed on Polyacrilamid or agarose gels and followed by silver and Ethidium Bromide staining. In RAPD method, polymorphic DNA band was cut on the gel followed by purification and then the segments were cloned in vector in Top10 strain of E.coli, and then sequenced. Meanwhile for Growth Hormone gene in Persian and Russian sturgeon the MEGA 4, Gene runner software were used to design the appropriate primers for PCR amplification. The PCR products were cloned in PTZ57R/T vector and transformed in Top10 E.coli strain and sequenced finally. For all other genes, similar methods were applied for PCR amplification and its products were sequenced and statistical analysis as well as phylogenetical tree was performed. In Single Nucleotide Polymorphism (SNP) method, after genomic library construction, in total 14.4 billion nucleotides were sequenced and similarity/ differentiation analysis of two species were investigated using specific bioinformatic software. Results indicated that Microsatellite and AFLP methods showed high level of genetic variation both within and between species. The Cytb gene, when 4 sample sequences from each species were compared two species were differentiated, however when analysis repeated over 15 samples, the sequence comparison couldn't differentiate two above mentioned species. Full sequence comparison of 16sDNA and mtDNA-ND5 gene showed variation in some nucleotide in both species of Persian and Russian sturgeon but no significant. Results of sequences obtained from cloned segment with RAPD method and also specific primer design based on produced sequences could succeed to discover a variable DNA band that able to differentiate two species from each other. Results of the present study also showed that the growth hormone gene (GH) of Persian and Russian sturgeon consists of 645 nucleotide that translate to 214 Amino Acids. The sequence comparison indicated that the gene coding growth hormone in Persian and Russian sturgeon had the highest similarity with GH of Mammals (71%), Anguilaformes (63%) and less similarity with bony fish (37%). Phylogenetic analysis indicates that Persian and Russian sturgeon in compare to other organism are ancient species and this gene is originated from a common ancestor. At present study the most appropriate results obtained from Single Nucleotide Polymorphism (SNP) method by sequencing 14.4 billion nucleotide from genome of two species of Persian and Russian sturgeon from North and the South Caspian Sea could prove that the Persian sturgeon is a valid and independent specie. This excellent results is the biggest scientific achievement for differentiation of two highly commercial important sturgeon species in the Caspian Sea in last two decades.
    Keywords: Biology ; Iran ; Caspian Sea ; 16S rDNA ; Acipenser persicus ; Persian sturgeon ; Acipenser gueldenstadttii ; DNA ; Russian sturgeon ; Species differentiation ; Molecular Marker ; SNP
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    A survey on health status of marine fish in Bandar-e-Imam station (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25189 | 18721 | 2018-09-03 17:16:20 | 25189 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Mariculture is one of the most important sub sector fisheries industry in Asia-pacific region. There are over 40 marine fish species commonly cultured, such as groupers (Epinephlus spp.), snappers (Lutjanus spp.) and Asian sea bass (Lattes calcarifer). But this industry in Southeast Asia experienced serious disease problem since the late 1980s. Khouzestan province has a coastal line about 200 km with many Creek and suitable area for Mariculture. Marine fish culture in Khouzestan province in floating net cages was successfully initiated in Ghazaleh Creek following development of Epinephlus coioides artificial seed production in the 1372. This study has been conducted since 1384 to 1387 in Bandar-e-Imam station. The aim of this research project was to determine the health management status in cage and hatchery, identification of marine fish pathogens (Bacteria, Fungi and parasite), examination of heavy metal in cages sediment and test the physico chemical factors of water in cages and hatchery. Different parts of broodstocks body and fingerlings including intestine, gills and body surface were examined. In this study, 18 Genus and species of bacteria such as Vibrio alginolyticus, Vibrio anguillarum, Vibrio splendidus, Vibrio vulnificus, Plesiomonas shigelloides, Aeromonas hydrophila, Aeromonas caviae, Pseudomonas sp. were diagnosed. Also 7 Genus and species of fungi such as: Aspergillus Niger, Aspergillus flavus, Aspergillus fumigatus, Penicillium sp. And Fusarium sp. Were isolated. In this survey following of disease appearance parasites such as; protozoans Amyloodinium sp. And Trichodina sp. In E.coioides and Acanthopagrus latus and Sparidentex hasta, monogean such as Benedenia sp. In E.coioides and S. hasta, isopoda probably Nerocila sp. In nasal cavity of E.coioides and copepods such as: Caligus sp. And Lernanthropus sp. In A.latus and S.hasta were identified. In this study, microorganisms (bacteria, fungi and parasites) isolated from three different marine fishes species, were reported for the first time in Iran. The range of the physico-chemical parameters of water in cages were: DO (5.42-10.24 ppm), BOD5 (1.51-10.24 ppm), No_2 (0.0197-0.89 ppm), No_3 (3.53-11.93 ppm), Po_4 (0.965-6.05 ppm), turbidity (4-58 NTU) and ammonia (0.0008-0.03 ppm). According to the results parameters such as nitrate, nitrite and turbidity were found more than standard levels. Heavy metals: Ni and Pb relatively high observed.
    Keywords: Biology ; Iran ; Khouzestan province ; Bandar-e-imam ; Mariculture ; Fisheries ; Species ; Epinephlus spp. ; Lutjanus spp. ; Lattes calcarifer ; Bacteria ; Fungi ; Parasite ; Aspergillus niger ; Aspergillus flavus ; Aspergillus fumigatus ; Penicillium sp. ; Fusarium sp. ; Vibrio alginolyticus ; Vibrio anguillarum ; Vibrio splendidus ; Vibrio vulnificus ; Plesiomonas shigelloides ; Aeromonas hydrophila ; Aeromonas caviae ; Pseudomonas sp. ; Grouper ; Snapper ; Sea bass
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  • 40
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    Study on Mahshahr creeks (north of Persian Gulf) as protected area (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25190 | 18721 | 2018-09-04 08:42:55 | 25190 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: In recent years MPAs (Marine Protected Areas) have been known as a flexible tool to improve fishery management as well as to preserve biodiversity in valuable coastal waters habitats. This study was conducted to selecting the sensitive and high potential area that are impacted by several environmental stress and associated habitats are being damaged in Mahshahr creeks in Northwest of Persian Gulf. Study was done in eight creeks from October 2005 to September 2006. Different biotic parameters such as phytoplanktons, zooplankton, macro zooplankton, benthic animals and fishes (trawled species) communities and also physico-chemical parameters and sediments heavy metals were investigated. Different parameters were sampled monthly or seasonal from each creek. Water samples with bottle sampler and sediment were collected by Peterson grab. Several ecological and biological indices based on heavy metals and benthic communities were used. Concentrations of heavy metals were measured by using voltammetric plarography method (Methrom 797). The range and mean concentrations obtained in mg/kg were 35.16-15.03 (27.01) for Cu, 171.41-65.57 (102.672) for Ni, 20.06-4.63 (13.22) for Co, 0.78-0.093 (0.22) for Hg, 379-65.07 (113.7) for Zn, 1.00-0.27 (0.559) for Cd and 29.72-7.09 (14.66) for Pb. To evaluate the levels of sediment contaminations, the background values of the different heavy metals were calculated and contamination factor for each metals and degree of contamination for each creek determined as well. Measured concentrations were compared with International standards. Ultimately the heavy metals contamination factors (Cf) can be arranged as follows: Hg〉 Zn〉 Cu≥ Ni〉 Pb≥ Co 〉 Cd and according to contamination degree (Cd), different creeks can be arranged as follows too: Ghannam〉 Ahmady≥ Zangy〉 Doragh≥ Darvish〉 ghazaleh〉 Patil〉 Bihad Regarding the results some elements such as Hg, Zn and Ni, are at risk level and all of the studied creeks are classified in moderate degree of pollution except Ghannam showing considerable degree of pollution. The benthic invertebrates are a well-established target in evaluations of environmental quality status. The AMBI (AZTI,s Marine Biotic Index) was developed to determine the impacts and the quality status in softbottom marine benthic communities. All creeks are characterized by muddy bottom. Macrobenthic animals, according to their sensitivity to an increasing stress gradient, classified in five ecological groups. In present study due to appearance of dominant species such as Capitella sp and nematodes (as opportunist species), diversity values was reduced. Two way ANOVAs showed only seasonal significant differences in mean abundance (P〈0.05, f=5.712, df: 3,21) and Richness index values (p〈0.05, f= 4.975, df=3,21), while all of creeks showed similar biological characters based on benthic communities. According to annual, mean of AMBI (BC) all of creeks classified in ecological group III with slightly pollution, except Darvish that was placed in unpolluted category. In general, according to AMBI and BI values, The most creeks are classified in unpolluted (34%) and slightly pollution (34%) categories except Zangy, Doragh and Patil in summer and also Zangy and Bihad in winter that showed moderate to heavily pollution (32%). The results of chemical quality of sediments, Cf values, Cd values, AMBI Index and water quality and risk Indices have confirmed each other. In general, the Mahshahr creeks are classified in moderate to heavy pollution status. In addition, biological parameters of benthic communities and other biotic parameters have showndescending trend in ecological quality in all of the studied creeks. Communities. According to annual, mean of AMBI (BC) all of creeks classified in ecological group III with slightly pollution, except Darvish that was placed in unpolluted category. In general, according to AMBI and BI values, the most creeks are classified in unpolluted (34%) and slightly pollution (34%) categories except Zangy, Doragh and Patil in summer and also Zangy and Bihad in winter that showed moderate to heavily pollution (32%). The results of chemical quality of sediments, Cf values, Cd values, AMBI Index and water quality and risk Indices have confirmed each other. In general, the Mahshahr creeks are classified in moderate to heavy pollution status. In addition, biological parameters of benthic communities and other biotic parameters have showndescending trend in ecological quality in all of the studied creeks. We used of some criteria for evaluation of biotic potential or sensitivity and also to explain the ecological health level of studied creeks. Positive and negative criteria are classified in three level 1, 3 and 5 for low, moderate and high degree of conservation value respectively. According to gained points the creeks Darvish, Doragh and Ghazaleh in high level and creeks Ghannam , Zangy and ahmady in low level of conservation value were classified.
    Keywords: Biology ; Ecology ; Iran ; Persian Gulf ; Khuzestan Province ; Mahshahr creeks ; Bottom sediments ; Heavy metals ; Contamination factor ; AMBI Index ; Risk Index ; Biodiversity ; Species ; Samples ; Benthic ; ANOVA ; Pollution
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    The Study of biology (age, feeding and, reproduction of Rutilus rutilus caspicus in south coast of the Caspian Sea (Iranian waters) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25569 | 18721 | 2018-10-06 17:07:25 | 25569 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The Caspian Sea is an important source of water in terms of having valuable resources of sturgeon and bony fish is taken into consideration roach(Rutilus rutilus caspicus (L.)) is one of the most important commercial species in the southern coast of the Caspian Sea in Iran , the catch of this species has declined substantially in the last 10 years, The management and exploitation need of comprehensive review of its biology .This study as part of a comprehensive study of feeding , spawning and growth of this species.In this study, using samples caught in beach seine along the southern coast of the Caspian Sea coastal ( Iranian waters ) took place. The fork length and total weight ranged between 12.5 to29.5(cm) and 29 to293( grm),10.5 to23(cm) and17.2 to21(grm) in Golestan and Gilan Province respectively. The b value of the length-weight relationship ranged 3.02 to 3.25 and 3.28 to 3.75 for female and male, in Golestan and Gilan Province respectively. The age composition of the catch was from 1 to 4 year in both Province, there was one spawning peak and Fecundity variations were high and ranged 7260 to 231965 eggs. Average growth in length was described with the Von Bertalanffy growth model: L (t) = 30.94(1-exp (0.42(t-0.18) and L (t) = 20.49(1-exp (0.53(t-1). The percent of empty stomach and prey dominant evaluated during different seasons by specific formula. Result showed that gastropod, Polychaete worms and molluscs were dominated and specific food items respectively. Shrimp, fish, insects, zooplankton and clams were scare of prey.
    Keywords: Biology ; Iran ; Caspian Sea ; Rutilus rutilus caspicus ; Spawning ; Feeding ; Growth ; Length
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    Biological survey on small pelagic fishes (sardine & anchovy) stocks in coastal waters of Hormuzgan province (Qeshm Island & Bandar Lengeh) with emphasis on sea surface temperature, plankton distribution and chlorophyll-a (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25198 | 18721 | 2018-09-05 16:25:43 | 25198 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Investigation on small pelagic fishes in the north of the Persian Gulf (Qeshm Island and Bandar Lengeh) coastal waters during October 2006 to September 2008, illustrated that 6 sardine and 2 anchovy species were locally existed. Sind sardinella (Sardinella sindensis) and Buccaneer anchovy (Encrasicholina punctifer) were dominated in this area. The peak of sexual maturity for Sind sardinella and Buccaneer anchovy occurred in spring and summer respectively. Reproduction period for Sind sardinella was from the middle of winter to late spring, and Buccaneer anchovy encompassed reproduction capacity all the year. The female: male ratio was calculated 1:0.7 and 1:0.9 for Sind sardinella, 1:1.27 and 1:1 for Buccaneer anchovy in Qeshm Island and Bandar lengeh sequentially. The mean of absolute fecundity were estimated 16234 and 16168 for Sind sardinella, 1277 and 1141 for Buccaneer anchovy in Qeshm Island and Bandar Lengeh respectively. L_M50 and L_C50 were calculated and the results showed both species mainly caught before length of maturity, and the mass populations have not sufficient opportunity to reproduction. Both species were planktonivore, theirs food prefect mainly consisted of copepods, naplius and bivalves. Vacuity index indicated both spices were semi-voracious appetite fishes. Based on calculated length-weight relationships in S. sindensis and E. punctifer, they have isometric growth. Growth parameters were estimated, the results illustrated that they were growth fast and short live species, which suggested S. sardinella and E. punctifer have 3 and 2 years maximum life span respectively. The recruitment pattern indicated double recruitment peaks per year in both species. Annually, four cohorts were distinguished in Sind sardinella and 3 cohorts in Buccaneer anchovy. Total mortality (Z), natural mortality (M) and fishing mortality (F) were estimated in both species, and the values of exploitation ratio (0.78 & 0.74) in Sind sardinella and (0.72 & 0.41) in Buccaneer anchovy were reasonable for current fishing effort due their short life span. Analysis of the results showed that CPUE in sardine and anchovy affected by reproduction and feeding processes. Results showed significant correlation between sardine CPUE and Sardine GaSI (P〈0.05, r= 0.499), similarly between anchovy CPUE and anchovy GSI (P〈0.05, r=-0.635). Further, significant correlation between sea surface temperature with sardine CPUE (P〈0.05, r=0.493), phytoplankton distribution (P〈0.01, r=0.560), anchovy GSI (P〈0.05, r=0.526), anchovy GaSI (P〈0.05, r=-0.500) and chlorophyll a (P〈0.01, r=0.780). Phytoplankton distribution with sardine GSI (P〈0.05, r=0.453), zooplankton distribution with anchovy GSI (P〈0.05, r=0.671), and chlorophyll with sardine GSI (P〈0.01, r=0.761) have significant correlation.
    Keywords: Fisheries ; Iran ; Persian Gulf ; Qeshm Island ; Bandar Lengeh ; Sardinella sindensis ; Encrasicholina punctifer ; Reproduction ; Feeding ; Growth and mortality ; Catch per unit effort ; Sea surface temperature ; Chlorophyll a ; Sexual maturity ; Plankton ; Naplius ; CPUE ; GSI ; Sardine ; Anchovy ; Coastal waters ; Surface ; Temperature ; Distribution
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    Study on physico-chemical characteristics of water in the southern of Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25563 | 18721 | 2018-10-05 16:51:28 | 25563 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: In this study, the characteristics of physico-chemical parameters of water done in coastal part of the southern Caspian Sea during 2010-2011 (four seasons) in 8 transects (Astara, Anzali, Sefidrour, Tonkabon, Noshahr, Babolsar, Amirabad and Torkman). For measurement of water quality parameters, 480 samples were collected in different water layers by using of APHA standards methods. According to modeling and statistical methods was used multivariate for evaluation of factor analysis (MFA) in general groups and discriminant analysis (MDA) for spatial and temporal with water quality parameters. The statistical procedure of MFA was used for data reduction and finally six factors selected with about 80.48 percent of total variances related to them. The statistical procedures of MDA were used for the role of spatial and temporal of water quality parameters in different water layer. The function one gave eight parameters (pH, salinity, EC, DO, NO^3-, water temperature, NH^4+ and N^-total) affording more than 80, 67, 80, 77 and 71 % correct assignations (return to the same transects) in spatial analysis. In all transects during four seasons, function one gave five parameters (water temperature, salinity, EC, NO^2- and NH^4+) to afford 100, 100, 100, 97 and 97 % correct assignations in temporal analysis. The results showed that, the average amounts in light penetration layers during four seasons were for EC(15.04±0.18ms/cm), pH(8.38±0.01unit), salinity (10.31±0.16g/l), DO(5.67±0.09ml/l) and water temperature (18.28 ±0.72°C). The average amount of nutrients for parameters such as organic and inorganic phosphorus, total phosphorus, NO^2-, NO^3-, NH^4+, N^-Total and SiO_2 were 0.50±0.02, 0.32±0.01, 0.82±0.02, 1.76±0.10, 0.10±0.01, 1.51±0.10, 46.82±1.80 and 8.60±0.29µM, respectively. The average amounts in dark layers during four seasons were for EC (15.46±0.38ms/cm), pH (8.34±0.03unit), salinity (10.70±0.35g/l), DO (4.55±0.13ml/l) and water temperature (9.62 ±0.16°C). The average amount of nutrients for parameters such as organic and inorganic phosphorus, total phosphorus, NO^2-, NO^3-, NH^4+, N^-Total and SiO_2 were 0.54 ±0.05, 0.33 ±0.02, 0.87±0.06, 1.92±0.21 , 0.08± 0.01, 1.55±0.25, 45.10±3.25 and 10.05±0.62 µM, respectively. The average amounts of light penetration in all transects during four seasons 4.49±0.38 meters. The maximum light penetrations during spring, summer, fall and winter seasons were 9, 8, 8.20 and 7 meters, respectively. The maximum water temperatures in light and dark layers were 32.36 and 11.50°C. Also, the maximum differences about thermocline temperature during summer and fall seasons were 19.6°C and 10°C, respectively in southern part of Caspian Sea. Data in spatial analysis were not significant (p〉0.05), it means if add or removed any transect did not change occurred. But in temporal analysis were significant (p〈0.01), and it means no way remove any seasons for a research work on Caspian Sea in southern part. In those transects with 100m depth. Water temperature is only highly effective parameter between other parameters. In function 1 between 3 functions in transects with 100m depth water temperature with 60% variance was the main role and effective between different seasons in depth part were very high.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Astara ; Anzali ; Sefidrour ; Tonkabon ; Noshahr ; Babolsar ; Physico-chemical parameters ; Nutrient maters ; Sea water ; APHA ; pH ; Salinity ; Temperature ; Nutrients
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  • 44
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    Geographical distribution of commercial bony fishes in Iranian waters of the Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25568 | 18721 | 2018-10-06 17:03:55 | 25568 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The beach seines catch and effort data from 1996 to 2011 categorized in four periods and used in this research. Geographical coordinates of beach seines determined by portable GPS and distribution maps of important and commercial species prepared by catch per unit effort (CPUE). The concentration of beach seines in the western coast of Golestan province, Kiashahr area and eastern part of Anzali was more than another part. The daily effort in Kiashahr and Anzali area was very high, thus the fishing stress in these areas was more than another part of Iranian coastal waters of the Caspian Sea. The number of species in catch composition increased from east to west and the beach seines of Guilan province had total of commercial species, but the number of commercial species rarely was five species in Golestan province and especially in eastern coast. Relative frequency and distribution of Kuttum in the east Babolsar area and then between Sepidroud and Anzali lagoon were more than another part. The ISI index of Kuttum was high in all of the area except east coast of Golestan province. The main distribution of Mullets determined in the western coast of Golestan province and the Amirabad fishing area and in these areas, the ISI index of Mullets was high. The main distribution area of common carp determined in east coast of Golestan and the ISI index of common carp was very low in other parts. Density and distribution of Kolmeh were high in eastern and western coasts of Iranian waters of the Caspian Sea and the density of this species was very low in Mazandaran province. The density of Zander was very high in surrounding of Sepidroud River and Anzali lagoon. The ISI index of commercial species in Guilan province was more than other provinces except Kuttum, mullets and common carp.
    Keywords: Biology ; Ecology ; Iran ; Caspian Sea ; Golestan province ; Guilan province ; Mazandaran province ; Babolsar ; Anzali ; Kiashahr ; Bony fishes ; CPUE ; ISI index ; Distribution map ; Species ; Fishing ; Kuttum ; Mullets ; Common carp ; Density
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    An ecological investigating on controlling Mnemiopsis leidyi population activity 6: molecular test in identification of Mnemiopsis leidyi (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25214 | 18721 | 2018-09-06 05:58:33 | 25214 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: In this study population genetic structure of Mnemiopsis leidyi were examined by 200 samples from the Caspian Sea. Samples were collected from Guilan province (Astara region), Mazandaran province (Amir Abad region), Golestan province (Gomishan region) and north part of the Caspian Sea. 50 samples were collected from each region. DNA was extracted by phenol - chlorophorm method and its concentration was 50 to 100 nanogram. PCR was performed using 18s rRNA gene. The PCR products of samples were digested by 12 restriction endonuclease enzymes. The digested products accompanied with standard marker (50 bp lader ). In order to measure fragment size samples were run on a 6% vertical polyacrylamide gel. The fragments were visualized by silver staining of the polyacrylamide gel. In this study, Dra I and Alu I enzymes showed different digestion pattern, as each of these enzymes had two genotypes. Other enzymes had similar digestion pattern. RAPD method used by 19 random primers. Ten of nineteen primers showed polymorphism patterns. Statistical analysis of data was performed by Popgene software. In this study, the maximum of genetic variation was in north of the Caspian Sea. Also, the maximum of genetic distance was between north area and Golestan coasts (0.089) and the minimum of genetic distance was between Mazandaran and Guilan coasts (0.001). The UOGMA dendogram showed two clusters. The samples of Mazandaran , Guilan and Golestan coasts placed in one cluster and samples of north area in other cluster The genetic diversity was significantly different between samples of north area and Golestan coasts (p≤0.05). As result, there is a significant genetic divergence between some of samples. Therefore, two genetic group of Mnemiopsis leidyi were identified in the Caspian Sea.
    Keywords: Biology ; Iran ; Caspian Sea ; Guilan province ; Mazandaran province ; Golestan province ; RFLP ; RAPD ; Mnemiopsis leidyi ; Genetic variation ; Population ; Molecular Test ; Samples ; Enzymes ; Genotype
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    Using biotechnology and chemical approach extraction of chitin and conversion to chitosan from the cyst shells of Artemia urmiana (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25618 | 18721 | 2018-10-07 16:34:53 | 25618 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Chitin and chitosan are 2 very important products of biopolymer that enjoy high consumption in industry, but their production sources are very limited. In this study, Artemia urmiana cyst shells were obtained from previously collected and stored ones in Iranian Artemia Research Center. 20 kg of Artemia urmiana cyst shells were sampled, cleaned, separated, dried and transferred to Iranian Artemia Research Center Laboratory to extract their Chitin and chitosan. Their chitin and chitosan initially were extracted using optimized common chemical methods. Their properties were compared to 2 other types of Chitin and chitosan obtained from crab and shrimp manufactured by Vietnam and China, respectively. To determine their quality, elemental analysis device, infrared spectrophotometry, x –ray radiography, determination of viscosity , molecular weight, crystallinity percent, color, de stylization measure, empirical and molecular formulas were made. The results showed that the percentage of chitin obtained from Artemia cyst Shells in Chemical method was 28 ± 3 % by weight and efficiency into chitosan (grade steel relief) in this method was 50± 5%. To optimize the extraction procedure and the removal of proteins of chitin by biological practices that were done by sodium hydroxide in the chemical method, it was replaced by the bacterium Bacillus subtilis. And in the bio- phase of chitosan de steelation fungus Aspergillus niger enzyme was replaced instead of sodium hydroxide at high temperatures. The results showed that chitin and chitosan can be extracted from Artemia cyst shell using biological method and their characteristics included as in chitin 49.6% C, 8.2 % N, 7.5 % H, and 34.5 %O. Also the same levels for chitosan were 44.4 %, 8.9, 7.2 and 39.5 %, respectively. Their other quality characteristics were included chitin average molecular weight 4.9×10^6 Dalton, crystallinity percentage of 36.4, viscosity at 20°C 31 centipoise and its color was gray to brown. In the biologic method, the average molecular weight of chitosan, crystallinity percentage, viscosity at 20°C, were 5.1×105 Dalton, 94.5, and 18 centipoises, respectively. Also, its color was pale brown. Chemical structure of extracted chitin and chitosan from the shell of Artemia urmiana cysts were C_7H_12NO_4 and C_6H_11 NO_4c, respectively. The comparison of chitin and chitosan obtained from each chemical and biological method revealed that replacing biological methods instead of chemical methods is possible in achieving these products at suitable condition and better quality. This can eliminate the use of chemicals damaging the environment such as sodium hydroxide and decrease environmental pollution.
    Keywords: Biology ; Chemistry ; Iran ; Artemia urmiana cyst shell ; Chitin ; Chitosan ; Chemical ; Biological methods ; Crab shell ; Shrimp shell ; Biotechnology
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    Determining of DNA-barcoding of 6 – 8 species by partial sequencing of mtDNA (CO1) using molecular method (PCR-Sequencing) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25619 | 18721 | 2018-10-07 17:03:03 | 25619 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Barcodes are short segments of DNA that can be used to uniquely identify an unknown specimen to species, particularly when diagnostic morphological features are absent. These sequences could offer a new forensic tool in plant and animal Conservation-especially for endangered species. It was proved that a small fragment of mitochondrial DNA from the 5′-end of cytochrome c oxidase subunit 1 (COI) gene as a reliable, quick and costeffective identification system for most Crustacea like shrimp. Take a look in DNA barcoding website show that there is a little data about Iranian shrimps which live in Persian Gulf and Oman Sea. In this Study six species of shrimp: Fenoro penaeus indicus, Fenoro penaeus merguensis, penaeus semisulcatus, Metapenaeus affinis, Marsupenaeus japonicas, Fenoro penaeus penicillatus were collected from different stations in Persian Gulf and Oman Sea. All materials were preserved in 70% ethanol and were shipped to the laboratory for taxonomic studies. After identification, the total DNA was extracted; COI gene was first amplified and then sequenced for each species. Finally the collected data were analyzed with the specific phylogenetic software. The results were amazing and the interesting part was that analytical methods for showing species relationship suggested that Fenoro penaeus penicillatus is closer to Marsupenaeus gnus than penaeus gnus. This finding needs more investigation to be proved. We suggest a workflow for DNA barcoding, including database generation and management, which will ultimately be necessary if we are to succeed to join universal DNA barcode for Crustacea.
    Keywords: Biology ; Iran ; Persian Gulf ; Oman Sea ; DNA ; DNA barcoding ; Species ; mtDNA ; CO1 ; Molecular method ; PCR ; Specimens ; Morphological ; Cytochrome ; Mitochondrial DNA ; Shrimp ; Fenoro penaeus indicus ; Fenoro penaeus merguensis ; Penaeus Semisulcatus ; Metapenaeus affinis ; Marsupenaeus japonicas ; Fenoro penaeus penicillatus
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    The study of feeding, reproduction and biological parameters of kilka fishes in the Iranian waters of the Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25622 | 18721 | 2018-10-08 05:50:41 | 25622 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The overfishing and changing environmental following the introduction of the comb jelly Mnemiopsis leidyi in the Caspian Sea, the population structure has a strong influence on kilka fishes in the Caspian Sea and therefore to in this investigation which had been done in commercial catch regions (where discharged theirs catch) in three ports Babolsar, Amirabad (in mazandaran) and Anzali (in Guilan), stimated catch and catch per unit effort, the age structur of catch, length- weight relationship, von Bertalanffy growth parameters, condition factor, sex ratios, maturity stags, spawning and feeding. The result shown that catch of kilka in Iranian coastal in 2012 decreased from 24080 ton to 22696 ton in 2013. But CPUE increased was 2.7 ton (Vessel ×Night) and there was no significant changes. Common kilka was the predominant all months of the year, 98.1 and 98.9 percent in 2012 and 2013 respectively. The mean length was 104.4±10.5 and 101.6±11.2 at the same time and range length 92/ 5 – 117/5 mm were the dominant population(about 75%). Relative abundance of fish less than 77.5 mm and more than 127.5 mm is very low. Age abundance during this period have not been substantially changed and always fishes with 3 and 4 year olds had the highest frequency73.6 and 73.5 percent respectively. The parameters of the Von Bertalanffy growth curve were (8.141) 048.1 for common kilka. The instantaneous coefficient of natural mortality (M) was 0.506 yr-1. The instantaneous coefficient of fihing mortality and total mortality were 0.694 yr-1 and 1.2 yr^-1 , respectively. The exploitation rate of common kilka varied during 2012-2013 between 0.41-0.50. The mean condition factor 1.41±0.14 (n=5801) and 1.38±0.16 (n=6754) at the same time respectively. According to statistical analysis Anova oneway were a significant difference between condition factor at the same time . Main prey common kilka was Acartia tonsa accounting for over 80%. It seems Common kilka due to a decrease in the frequency of other zooplankton species depend more than ever on Acartia tonsa and this species is probably a concentration of prey for Common kilka.
    Keywords: Biology ; Fisheries ; Iran ; Caspian Sea ; Mazandaran Province ; Guilan Province ; Anzali ; Babolsar ; Kilka fishes ; Age ; Growth parameters ; Condition factor ; Exploitation rate ; Feeding ; Reproduction ; Overfishing ; Comb jelly ; Mnemiopsis leidyi ; Population ; Sex ratios ; Maturity ; Spawning ; Mortality ; ANOVA ; Acartia tonsa
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    Identification of qanats in Rafsanjan, Sirjan and Ravar cities for fish culture (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25229 | 18721 | 2018-09-06 06:14:35 | 25229 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Study and recognition of the Quanat water of Rafsanjan, Sirjan and Ravar cities has been beginning since the November of 2004. Among 228 Quanat chains were studied in these cities, 165 quanat chains had a discharge more than 10 liter per second (L/Se) which were sampled. In this research oxygen, pH, discharge, water temperature and air temperature were measured in two times and in the local place. Height from the sea level, longitude and latitude were measured (by GPS Machine) in one time and in the local place and other physical and chemical factors including No_2, No_3, Co_2, total hardness, NH_3 and So_4 were measured one time in the laboratory. In this manner, the condition of connection ways of considered Quanat with city was studied. With examination on the Quanat water in above said cities, all of Quant was within desired extents for aquaculture activities. Moreover in some of them, water temperature was higher than 20 degree centigrade that with some changes and in specific months can be used in aquaculture.
    Keywords: Biology ; Iran ; Rafsanjan ; Sirjan ; Ravar ; Fish ; Aquaculture ; Quant ; Water ; Oxygen ; pH ; Temperature ; No2 ; No3 ; Co2 ; NH3
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    The survey statistical and biological of sturgeon in the southern Caspian Sea (Mazandaran waters) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25627 | 18721 | 2018-10-07 16:48:29 | 25627 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This study have been conducted entitle biological and statistical sturgeon in the southern Caspian Sea (Mazandaran waters)” from 2009 to 2013. Sampling was manthly. Overal, 1859 specimens of different species of sturgeon was caught including Acipenserpersicus (69%), A. stellatus (19%), Husohuso (6%), A. nudiventris (4%) and A. guldenstaedtii (2%). Totally, the meat and caviar of five species were found 53263.6 kg and 5633 kg respectively and caviar to meat ratio was 10.6%. The results show that ration of meat and caviar whole species reduced from 2009 to 2012 in which the meat and caviar of A. persicus was declined from 12146 to 4465 kg in meat and caviar from 1358 to 487 kg were extremely reduced respectively. The CPUE (catch per unit effort) of sturgeon has fluctuated from 2009 to 2012. Totally, the CPUE of A. persicus was decreased from 0.150 (2009) to 0.130 (2012) kg boat per day, A. stellatue from 0.009 (2009) to 0.021 (2012) kg boat/day, A. guldenstaedtii from 0.002 (2009) to 0.003 (2012) kg boat/day, A. nudiventris from 0.027 (2009) to 0.001 (2012) kg boat/day and H. huso from 0.123 (2009) to 0.018 (2012) kg boat /day. 1061 specimens of fish has been caviar including A. persicus with 69%, A. stellatus (18.1%), H. huso (5.5%), A. guldenstaedtii (2.7%). The frequency of caviar categorize was included 1 , 2, 3 and massive with 49.6%, 38%), 10.6% and 1.9%, respectively. The highest of meat and caviar belong to A. persicus with 31414 kg and 3515.7 kg, respectively were found and the lowest was 841kg and 105.9 kg pertain to A. guldenstaedtii. The results show that 65.9% of sturgeon was caught by legal catch (landing) and 34.1% by beach seine remained. 391 individuals was age determined that the minimum and maximum age was 9 and 41 year comprised to H.huso and A. stellatus, respectively. A. persicus and A. stellatus were majority caught compare to other species. A. persicus has the highest frequency in 14-15 year age groups with 50% and 57% in 2009 and 2011, respectively and 15-16 year age group with 41% and 56% in 2010 and 2012, respectively. The results show that mean of fork length, body weight, caviar weight of A. persicus was declined because the FL and weight average (±SD) obtained from 152.4±16.7cm and 25.8±9.2 kg in 2009 to 146.5± 18.7cm and 22.1±9.2kg in 2012, respectively. In contrast, the average (±SD) FL and weight of H. huso was increased from 218.8±41.1cm and 137.3±96.6kg in 2009 to 231.3±45.2cm in FL and 146.2±78.5kg body weight in 2012, respectively. Consequently, the results show that stocks of sturgeon in the Caspian Sea has a trend declined because of some species in IUCN categorize list was Endangered in 2006 but at this moment lie in Critically Endangered list of IUCN categorize were down which could be a serious alarm for the valuable sturgeon fish in the Caspian Sea. Therefore, recommendation that trade catch of sturgeon in the Caspian Sea must be forbidden and continue legal catch for spawner fish that needede to artificial propagation only.
    Keywords: Biology ; Iran ; Caspian Sea ; Biology ; Sturgeons ; A. persicus ; A. stellatus ; A. guldenstaedtii ; A. nudiventris ; H. huso ; CPUE
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    Genetic national plan study in order to shrimp breeding and developing of shrimp culture technology (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25256 | 18721 | 2018-09-07 07:21:26 | 25256 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Sampling was done using 90 post larvae which were produced by reproduction of some broodstock of Penaeus indicus in one day and reared in the same situation for 4 month. Samples were classified in 3 group high growth, medium and low (according to their weight and length). Genomic DNA was extracted from a 1cm^2 piece of muscle using the phenol-chloroform method. The polymerase chain reaction (PCR) was done using 21 RAPD loci. And PCR products were separated on 3% Agarose gels. From 21 studied loci, 12 produced polymorphic bands. The most polymorphic band produced using OPAQ 9 and the least by OPAQ 7. According to Nei 1972, the highest distance (0.457) was between low growth group and medium and the lowest (0.091) between high growth group and medium, therefore the highest identity (0.912) was between high growth group and medium and the lowest (0.633) between low growth group and medium. Consensus neighbour-joining tree using Nei (1972 and 1978) resulted in two clades, the first including high and medium growth groups and the second low growth group, it appears that low growth group are depended on separated population of the two others. With considering of mean weight of F1(16.25±1.5), mean weight of 15 ±1.2 and mean weight of parent 31.6, response to selection (R) and heritability for growth in this species were estimated 1.2±0.2 and 0.07±0.01 respectively. In another part of this study Sequencing of specific bands and primer design were done and examining of them on the same age specimens is necessary in following.
    Keywords: Biology ; Iran ; Penaeus indicus ; High growth ; RAPD marker ; Genetic ; Shrimp ; Breeding ; Culture ; Sampling ; Broodstock ; Phenol-chloroform methods ; Population ; Species ; Specimens
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    Broodstock management, reproduction, cryopreservation in salmonid, genetic management and selective breeding in responsible aquaculture (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25651 | 18721 | 2018-10-14 02:48:20 | 25651 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This study was conducted to codify the guideline and indexes of broodstock management in salmonid fish and cryopreservation, selective breeding and sex reversal in hatchery to attain responsible aquaculture development. Cryopreservation includes of sperm and egg in term of economic is important in aquaculture. In this report mentioned the type and name of material. All female and sex reversal fish are produced by steroid hormone which mentioned in this report. Selective breeding include individual selection and family selection which could be used for genetic improvement of fish.
    Keywords: Aquaculture ; Biology ; Management ; Iran ; Broodstock ; Reproduction ; Cryopreservation ; Genetic ; Breeding ; Aquaculture ; Salmonid fish ; Sex reversal ; Hatchery ; Sperm ; Egg ; Female ; Steroid hormones
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    Extraction‚ purification, identification and amount verification of steroids in Sargassum glaucescens and Padina boergesni algae in Oman Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25642 | 18721 | 2018-10-14 02:40:17 | 25642 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Padina boergesenii is one of the most abundant brown algae distributed in the north of Persian Gulf and Oman Sea. In this study after sampling and preparation of Padina boergesenii by Chroform-Etanol (3-1) solvent and by Methanol has been extract. Separation and purification of the compounds was carried out using thin layer, general and inverse column chromatography, Cephadex and high-performance liquid chromatography (HPLC). Structural elucidation of the constituents was based on the data obtained from H-NMR, 13C-NMR, HSQC, HMBC, DEPT and Cephadex LH-20. The steroids compounds separated from above alga were identified as 22dehydrocholesterol (1), cholesterol (2), fucosterol (3), β-sitosterol (4), stigmasterol (5), ostreasterol (6) and two epimer of hyroxyestrol(7), based on their spectral data and from comparison with those previously reported in the literature.
    Keywords: Biology ; Chemistry ; Iran ; Oman Sea ; Persian Gulf ; Brown Algae ; Padina boergesenii ; Steroids compounds ; Extraction ; Purification ; Identification ; Amount verification ; Sargassum glaucescens ; Algae ; Abundant ; Sampling ; Chromatography ; Cephadex ; Dehydrocholesterol ; Cholesterol ; Fucosterol ; β-sitosterol ; Stigmasterol ; Ostreasterol
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    Trout farms and other human activities effects on Cheshmehkileh river ecosystem in Tonekabon (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25792 | 18721 | 2018-10-13 08:54:26 | 25792 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Cheshmehkileh River and adjacent mountainous streams, play a strategic role as a historical axis for anthropogenic civilization, human welfare also habitat and migration pathway of commercial – biologic valuable fishes e.g. Caspian trout, Caspian kuttum, members of Cyprinidae family in south Caspian Sea drainage. Treats such as overfishing of Caspian trout and Red spotted trout stocks in mountainous headwaters, barriers construction and manipulations those are out of river carrying capacity developed by human activities, affected normal function of river as well. Sand mining big factories establishment next to the river, legal and illegal trade of river sediments, direct entry of Tonekabon landfill leakage into the river, development of Rainbow trout farms since 3 decades and huge effluents into the river containing dead fish and types of solids, escapement of cultured Rainbow trouts into the river, … are major minimum factors which needs basic information for integrating inclusively drainage management system. Cheshmehkileh River contains Headwaters of Dohezar (Daryasar & Nusha), Sehezar and Valamroud rivers during 13 monthly sampling phases between September 2009 and October 2010 based on macrozoobenthoses investigations by EPT, EPT/C EPA protocols, measurements of nominated physic-chemical and microbiologic parameters. Probability of Rainbow trouts escapement and invasion, existence, nutrition in Cheshmehkileh environment indeed investigated. Data analysis explained significant differences (P〈0.05) between groups of measured parameters in different sampling stations. Dendogram of clustered analysis based on consolidation of major biologic/ physic-chemical and microbiologic parameters, separated stations No. 1, 3, 2, 4 in one group and remained classified in different groups. Station 8 and 9 similarly separated which expressed general similarities according to Sehezar river environment which were differs in comparison with other stations. Station 11 separated according to its natural quality of water and environment. Similarities between station 10 to Sehezar river stations 8 and 9 expressed general influence of Sehezar River more than Dohezar River in Cheshmehkileh condition especially in station No. 10. High scores of EPT and EPT/C indices in upstream stations 1, 3 and 8 also low score of indices in stations 7, 13 and 6 expressed levels of environment quality between these groups of stations. Maximum average biomass of macroinvertebrates belongs to Trichoptera order in Cheshmehkileh River. Significant decrease of biomass in stations 11, 12 and 13 in comparison with other stations stated environment degradation in mentioned stations relevant to excessive sand mining as well. Pollution resistant groups of invertebrates significantly increased in downstreams against upstream stations. Also disappearing of Plecoptera order in station No. 7, 9, 10 and 13 stated low quality of environment in comparison with upstream stations. Confirmation of effects quality and quantity for point and non-point sources of imported pollutants require specific management considerations in order to present exploitations, pollutants control and emergencies for river monitoring in forthcoming years.
    Keywords: Aquaculture ; Management ; Iran ; Tonekabon ; Cheshmehkileh ; River ; Pollution ; Aquaculture ; EPTC ; Assessment ; Macroinvertebrates ; Chemistry ; Microbiology
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    Ecological study on the Persian Gulf Martyres Lake, Tehran (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25818 | 18721 | 2018-10-13 10:23:49 | 25818 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The ecological study of the Persian Gulf Martyres Lake carried out at the 5 stations between 2013 and 2014. This study identified 35 phytoplankton taxa. The diatoms taxa was dominated and their abundance recorded highest (2000 000 cell. L^-1) in the lake. The annual phytoplankton abundance was measured as 2500 000 cell. L^-1 during the study. The total nitrogen and water temperature were the significant a biotic parameters to increase cyanophytes abundance. Furthermore, 37 zooplankton taxa were identified. The Rotatoria abundance was dominated zooplankton. The annual zooplankton abundance was measured as 72 ind.l-1. Based on the CCA, there was no correlation between Rotatoria abundance and a biotic parameters. The study benthos showed Ephemeroptera and Diptera abundance were dominated; artificial bottom and lack of sediment and organic matters were the main reasons in decreasing of the benthos density. The Chitgar lake is the poorest lake in Iran due to low density of plankton and benthos organisms. The estimation of fish production was 123 kg/ha and for the lake was determaind 16 tonne. The finding display, invasive species was the main fish Chitgar lake that would be negative effect and increase eutriphication trend in the lake. Phosphorus parameter was limited parameters and trophy level recorded low due to high N/P ration in the lake. In overall the Chitgar lake situation is in Oligotrophic category with the low trophy level.
    Keywords: Ecology ; Iran ; Persian Gulf ; Chitgar Lake ; Phytoplankton ; Zooplankton ; Benthose ; Trophy ; Abundance ; Nitrogen ; Temperature
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    Study of benthic macro invertebrates in the Anzali lagoon (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25822 | 18721 | 2018-10-13 10:12:48 | 25822 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The Anzali wetland (37.28N – 49.25E) with less than 100 km2 surface area is located in the southwestern shores of the Caspian Sea in the Guilan province. The benthic macro-invertebrates were studied as a part of a main project titled study of aquatic communities variation in the Anzali wetland. The sampling was performed at the 10 stations with using a Van-Veen grab during 2014-2015. The results showed, 14 macro-invertebrates taxa were identified as Chironomidae (Insecta, Diptera) and Tubificidae (Annelida, Oligochaeta), they were dominated in the wetland. The maximum and minimum densities of macro-invertebrates were observed with 609±1119 and 93.32±27.92 n/m^2 respectively at the stations 5 and 1. The silt-clay constitute more than 85 percentage of the wetland sediments with maximum of 93.2±27.92 percentage at the 2 station and minimum 72.21±27.92 at the 5 station. Kruskal Wallis non parametric test (sig. ≤0.05), revealed significant differences for all studied factors at stations while of %tom and grain size had no significant variation. The CCA analysis revealed no accordance of total organic matter (TOM ) and silt- clay percentage vectors with Tubificidae and total benthic frequencies, while Chironomidae frequency is in accordance. Intensifying eutrification process in the Anzali wetland has affected its biota as the benthic fauna are almost limited in to most tolerant taxa which reflect the day after day increasing pollution the Anzali wetland.
    Keywords: Biology ; Ecology ; Iran ; Caspian Sea ; Guilan Province ; Anzali wetland ; Macroinvertebrates ; Eutrification ; Aquatic ; Tubificidae ; Chironomidae ; Insecta ; Diptera ; Annelida ; Oligochaeta ; Benthic fauna
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    Investigation of chromosome and ploidy levels of rainbow trout produced from imported eyed eggs (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25825 | 18721 | 2018-10-13 10:43:05 | 25825 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Rainbow trout is the main cultural species of coldwater fishes in iran. Often, aquaculturists intend to breeding in order to production of lines with higher growth rate potential and disease resistant. Nevertheless in the country, no trout breeding programs, has been performed yet and most of the farms focused on the cultivation of the first (unbred) race. While European countries progressed in trout breeding techniques and production lines with higher growth through genetic manipulation (chromosomal number and type changes of fish) and/or selection and their fish products derived from this technology, including eyed eggs and so on have sold to other regions of the world (eg: Iran). In this study, some biological parameters including survival, growth, feed conversion ratio (FCR.) and chromosomal number of two juvenile groups from imported( group 1) and native(group 2) eyed fish eggs were compared. For chromosomal investigation, blood smear test and flow cytometry were performed. sults showed a significant difference (P≤5%) in growth rate of native fishes and French group Native fish feed conversion ratio (0.9) was significantly difference (P≤5%) from that of French fishes (1.15). Chromosomal analysis showed no difference in chromosome number in treatments and two fish groups were 2n chromosome. Based on the results, the fishes of group 1 had faster grow potential and gain weight in less time than that of group 2 and this has been achieved to go through the process of selection and feminization without any change in number of ploidy. Whereas the ability of native fishes in food efficiency ( lower FCR.) was better. However, the reduction of rearing period is the benefit and preference of cultivation of imported or origionally foreign.
    Keywords: Aquaculture ; Biology ; Iran ; Imported eyed egg ; Native fish ; Ploidy level ; Growth ; Rainbow trout ; Chromosome ; Species ; Breeding ; FCR
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    Induction of population triploid-interploid in rainbow trout (Oncorhynchus mikiss) using by indirect (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25827 | 18721 | 2018-10-13 10:47:16 | 25827 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Induced polyploidy is a suitable tool for producing sterile fish which made commercial benefits in the aquaculture industry. This study carried out in order to produce triploid-interploid population via mating tetraploid female with diploid male rainbow trout. Heat shock was used for making tetraploid population and the best temperature and induction time were examined. Result showed that highest mortality from 1 day after fertilization to emerging were in groups 6 of embryos (40.1%) and the lowest were in groups 3 (33 %). Flowcytometry results showed that some fish were polyploidy. Comparative analyze of genome levels in tetraploid fish to control fish (diploid) and hen as standard indicator, confirmed tetraploid fishes in this study. In conclusion we can state that heat shock induction for 7 minutes at 65 hour –degree after fertilization in 28ºC is optimum temperature for inducting tetraploid rainbow trout.
    Keywords: Aquaculture ; Biology ; Iran ; Sexual development ; Triploid interploid ; Tetraploidy ; Heat shock ; Flow cytometry ; Rainbow trout
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    A survey on biodiversity of Cyprinid fishes of Iran (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25832 | 18721 | 2018-10-13 10:50:13 | 25832 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: A survey on Biodiversity of Cyprinidae family in Iran Cyprinidae family is the biggest and most important fish families with 367 Genera and 3006 species in the world. Members of this family is the most important freshwater species are present in a variety of freshwater ecosystems. Some of them can also live in brackish waters. Iran has about 93 species in the family Cyprinidae and consist half of the fishes in inland waters. In the past two decades many changes occurred in aquatic ecosystems, Fish habitat conditions changed and a lot of them are affected and some species are endangered or put under pressure. Restricted fish migration, Some Exotic species and have been entered in water resources and have narrowed arena on continued existence of native species. On the other hand, climate change and drought on water resources aggravate existing conditions. It is necessary effects of these changes in the family Cyprinidae be reviewed and analyzed.
    Keywords: Biology ; Ecology ; Iran ; Cyprinidae ; Biodiversity ; Water resources ; Climate Changes
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    Survey of potential adaptation (ion- osmotic) rainbow trout (Oncorhynchus mykiss) fingerling with Caspian Sea water (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25833 | 18721 | 2018-10-13 10:28:58 | 25833 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This study aimed to determine domain the adaptability of rainbow trout(Oncorhynchus mykiss) fingerlings in fresh water up to 20 per thousand (grams per liter) for providing facilities for growing this valuable species in the aquatic environment with salinity unconventional been completed. For this purpose the biochemical, bloody and physiologic parameters rainbow trout were studied to determine the adjustment range .Fingerlings fishes from cold water fish farm in the province Mazandran were prepared and for experiments were transferred to the Ecological Institute of Caspian Sea . Fingerlings with an average weight 31.56 ± 0. 07 SE g and average fork length 13.80 ± 0.15 SE cm, in 3 treatments in water with salinities (fresh, 13 and 20 grams per thousand) with a density of 15 numbers in polyethylene to 300-liter tank containing the 250 liters of water testing were introduced. Fresh water from Tajan rivers and water psu13 from the water Caspian Sea and water psu20 by mixing water the Caspian Sea and Sea salt was prepared . The daily amount to 50 percent of the of water tankss been replaced .During the experimental period was for 7 days and were not fed during the experiment.The water parameters was measured during the experiment included 6 ppm dissolved oxygen, pH equal to 8.2 and temperature 15.5 ° C . In the experimental period were not observed Losses in the experimental groups . The results showed that fish gill and kidney introduced in different salinities by making appropriate changes in chloride cells in the gills through increasing the number and the volume of these cells at the base of secondary blades and tubules in the kidney tubules to create greater interior space, are adapted to By changing salinity. Relatively parameters osmolarity, sodium, chloride, magnesium, cortisol, calcium, hematocrit, hemoglobin, number of red blood and white cells in water saltier than freshwater environment was higher (0.05〈 p, Duncan). Changes in hematological and blood plasma ionic parameters and vital organs Fingerlings indicates a willingness adaptability and the ability physiological adaptation fingerling was consistent with changes to environmental salinity brackish water .So, the factors measured with increasing salinity the uptrend that the range of variation for the osmolarite 449-281 mOsmol kg, for sodium, chloride, magnesium, cortisol, respectively, 211- 151, 165121, 3/3 7/0, 87. 53 mmol and the calcium 22-13 mg per dL. The measured values for hematocrit 32.2- 38.8%, hemoglobin 6.2 - 8.6 g per deciliter and the red and white blood cells, was respectively, 1.2-1.7×106 and the 15.6 -18.9×103.
    Keywords: Aquaculture ; Biology ; Iran ; Caspian Sea ; Adaptation ; Rainbow trout ; Gills ; Kidney ; Survey ; Oncorhynchus mykiss ; Fingerlings
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    Eutrophication trend of southeast Caspian Sea water (Mazandaran-Goharbaran) based on environmental and biotic parameters using single and multiple variables in order to feasibility of marine cage culture (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25839 | 18721 | 2018-10-13 10:48:08 | 25839 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The aim of this project is to study water quality and the trophic status at different months and depths in the southeast Caspian Sea-Goharbaran based on environmental parameters and nutrients during 2013-2014 in order the feasibility of fish cage culture. Results showed that water temperature, transparency, pH, dissolved oxygen, BOD5, COD, total alkalinity and TSS were varied 9.00-29.00C, 0.50-12.00 m, 8.05-8.74, 5.76-12.85, 14-101, 21-195 and 0.00-0.12 mg/l at the different months and depths, respectively. In addition, the values of NH^4+, NH_3, NO^2-, NO^3- and PO^4-3 were 0.007-0.051, 0.001-0.010, 0.002-0.015, 0.043-0.477 and 0.014-0.077 mg/l, respectively. Chl-a concentration was recorded as 0.060-8.02 mg/l. Scaled and unscaled trophic indices were equal to 3.42-5.52 and 2.61-5.85, respectively. The proper temperature for cultivation of salmonids species in this area began from October to March. The range of the standard of pH and dissolved oxygen level for fish farming cage were 7.80-8.50 and 〉5 mg /l that in current study results of pH and dissolved oxygen which were consistent in the current study. Various forms of nitrogen (ammonium, nitrite and nitrate ) at the different layers were acceptable and less than the threshold limit of Australia and New Zealand. The results of the nutrients concentrations compared with data from the reference years (1996-1996 ) in the Caspian Sea (Goharbaran region) showed that this ecosystem passed from oligotrophic status and shifted to mezotrophic and eutrophic condition. As a conclusion, although nutrients concentrations were acceptable in terms of fish farming cages in this region, some parameters such as chemical oxygen demand and transparency are not suitable for aquaculture industry. Meanwhile, based on various trophic levels, this ecosystem (Gohrabaran region) has a mezotrophic status with a high risk of eutrophication.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Goharbaran ; Physico-chemical parameters ; Water ; Fish cage culture ; Temperature ; Transparency ; pH ; Dissolved oxygen
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    A study on fishing status and some reproductive characteristics of Klunzinger’s mullet (Lize klunzingeri) in coastal waters of Khuzestan province (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25845 | 18721 | 2018-10-13 08:35:24 | 25845 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Biological characteristics of Liza klunzingeri were studied in two coastal areas, Sajaphi and Bahrekan, of eastern Khuzestan during March to February 2007. Among total 1880 measured fish specimens, 947 specimens were analyzed. The mean value of Gonado-somatic Index (GSI) for the male and female fish were calculated as 0.96± 1.39 and 3.25 ± 3.26 respectively. The GSI value was highest in November and lowest in July. The mean value of condition factor (K) was 1.25± 0.14 in male and 1.21± 0.15 for female. The highest K value were observed in June and the lowest value in February. The lenght at first maturity regardless of sexuality, was found to be 14.5 cm and the time of spawning based on reproduction pattern were determined in Nov- Dec. The length-weight relationship were calculated as Y=0.024L^2.76 (n=226R2=0.72) for males, Y=0.011L^3.00 (n=444R2= 0.78) for females and Y=0.0208L^2.82 (n=670R2 =0.82) for total fishes and also it’s found significant in level length weight relationship in (P〈0.05). According to biological characteristics and referring to American fisheries society (AFS) indices and Fuzzy logic expert system, Lize klunzingeri is classified as low vulnerable species.
    Keywords: Biology ; Iran ; Khuzestan province ; Sajaphi ; Bahrekan ; Lize klunzinger ; Gonado-somatic Index ; GSI ; Condition factor ; Biological characteristics ; Female ; Male ; Specimens ; Fisheries
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    Thalassoma lunare (Linnaeus, 1758) (2021)
    Iranian Fisheries Science Research Institute
    In:  http://aquaticcommons.org/id/eprint/21097 | 17408 | 2017-04-27 06:20:06 | 21097 | Islamic Azad University, Science and Research Branch, Tehran
    Details
    Publication Date: 2021-07-14
    Description: Thalassoma lunare is also named the Moon Wrasse because of its yellow caudal fin shapes like crescent moon with long upper and lower lobes. It is an inhabitant of coral reefs and surrounding areas at depths from 1 to 20. T. lunare is the most abundant wrasse species in the Persian Gulf. This species can reach 16 cm in total length in Iran, Persian Gulf, Kish Island. Moon wrasses are active fish, said to be moving all day long. They are also territorial, nipping, chasing, and otherwise harassing fish that get in their way. Being diurnal, wrasses have strong vision, although they also have a decent sense of smell. At night, they rest in niches often under rocks or other such structures. If needed, a moon wrasse may dig out a space under a rock by repeatedly swimming through it until it fits without struggle. They are protogynous hermaphrodites, all starting off as females and changing to males, a process which, for the moon wrasse, takes only 10 days. Some moon wrasses live in groups consisted of a dominant male, and a "harem" of about a dozen other wrasses, some female and some male. The alpha male is more brightly colored, and at every low tide hour, changes from green to blue, and goes into a show of attacking and nipping all the other wrasses. This is his way of showing his dominance to the rest of the males and keeping the females in check. During breeding season and before high tide, the alpha male turns completely blue, gathers up every single female, and the spawning frenzy begins.
    Keywords: Biology ; Ecology ; Fisheries ; Thalassoma lunare
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    Megalomma vesiculosum (Montagu, 1815) (2021)
    Iranian Fisheries Science Research Institute
    In:  http://aquaticcommons.org/id/eprint/21105 | 17408 | 2017-04-27 07:28:58 | 21105 | Islamic Azad University, Science and Research Branch, Tehran
    Details
    Publication Date: 2021-07-14
    Description: Megalomma vesiculosum (Giant Feather Duster Worm) has reported for the first time from Persian Gulf (Iran, Kish Island). M. vesiculosum is a polychaete worm that reaches up to 12 cm in length. It has relatively few (about 50) tentacles, each with a large eyespot at the free end. It creates a long and tough tube, which is mostly encrusted untidily with large shell fragments and small stones. The tube protudes no more than 40-50 mm (in Iran, Kish Island) from coarse sand or muddy gravel at low water and below. The body and crown vary in color from yellowish to a reddish-purple brown and is usually spotted. The Giant Feather Duster Worm is a relative of the garden earthworm. It uses its colorful tentacles to filter the water for food, which includes phytoplankton and bacteria. M. vesiculosum requires a high density of phytoplankton and dissolved particulates to survive in a marine ecosystem. The worm generally buries its tube in the sand and the crown protrudes above to strain the water. If this worm sheds its tentacle crown, it is not getting enough food. It will usually regrow another, smaller than the previous. If this cycle continues, the worm will starve. Members of the class Polychaeta are mostly gonochoric (sexual). Females produce a pheromone attracting and signalling the males to shed sperm which in turn stimulates females to shed eggs, this behavior is known as swarming. Gametes are spawned through the metanephridia or body wall rupturing (termed as ‘epitoky’, wherein a pelagic, reproductive individual, ‘epitoke’, is formed from a benthic, non reproductive individual, ‘atoke’). After fertilization, most eggs become planktonic; although some are retained in the worm tubes or burrowed in jelly masses attached to the tubes (egg brooders). Eggs develop into trocophore larva, which later metamorph into juvenile stage (body lengthened), and later develop into adults.
    Keywords: Biology ; Fisheries ; Megalomma vesiculosum
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    Sabellastarte spectabilis (Grube, 1878) (2021)
    Iranian Fisheries Science Research Institute
    In:  http://aquaticcommons.org/id/eprint/21106 | 17408 | 2017-04-27 06:38:05 | 21106 | Islamic Azad University, Science and Research Branch, Tehran
    Details
    Publication Date: 2021-07-14
    Description: Sabellastarte spectabilis is commonly known as the feather duster worm, feather duster or fan worm. It has reported for the first time from Iranian waters (Kish Island) and finding in the intertidal and subtidal reefs. Especially common in sites where phytoplankton is abundant. The worm's body occupies a flexible mucus tube formed by adhesion of silt from the water column. Polychaetes, or marine bristle worms, have elongated bodies divided into many segments. Each segment may bear setae (bristles) and parapodia (paddle-like appendages). Some species live freely, either swimming, crawling or burrowing, and these are known as "errant". Others live permanently in tubes, either calcareous or parchment-like, and these are known as "sedentary". This large worm can reach 80 millimeters in length and 10–12 millimeters in width. It is buff in color with purple specks. It lives in a tough, leathery tube covered with fine mud. The tentacles are striped in dark and pale brown bands and bear neither stylodes nor eye spots. There are two long, slender palps and a four-lobed collar. Cilia on the tentacles cause currents in the water and organic particles are caught as they float past. They are channelled along mucus-filled grooves to the mouth. Larger non-food particles are used for building the tube. The tentacles are also used as gills for gas exchange. This worm can reproduce asexually by fragmentation, and can regenerate body parts after being damaged. Reproduction can also be by sexual means. Most worms are either male or female and the gametes mature in the coelom before being released into the water column. Some specimens, particularly larger ones, have both male and female gametes and a study concluded that this was consistent with sequential hermaphroditism. Fertilization is external, and after a short time in the plankton, the trochophore larvae settle out and grow into adult worms.
    Keywords: Biology ; Fisheries ; Sabellastarte spectabilis
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    Pseudoceros prudhoei (Newman & Cannon, 1994) (2021)
    Iranian Fisheries Science Research Institute
    In:  http://aquaticcommons.org/id/eprint/21107 | 17408 | 2017-04-27 06:39:06 | 21107 | Islamic Azad University, Science and Research Branch, Tehran
    Details
    Publication Date: 2021-07-14
    Description: Pseudoceros prudhoei is usually found on rocky or reef habitats, swimming in open waters or under coral rubble plates, where they find their prey, the ascidians. Maximum length is 5 cm in Persian Gulf (Iran, Kish Island). While some of the free-living flatworms tend to hide under rocks escaping from the sunlight, colorful polyclads usually demonstrate a very conspicuous behavior, swimming through the water column next to coral reefs. These animals actually do not need to avoid predators, because their bright color pattern works as a warning sign about their distastefulness and toxicity. None of the animals had a negative response to the presence of light. When the flashlight was turned on, they started swimming around and exploring the environment and none of the worms hid under the rocks searching for cover. In fact, the light didn’t seem to bother them at all, as some would swim toward it, swim back and settle down at any spot. P. prudhoei is not negatively phototactic and reinforce the function of aposematic coloration in pseudocerotids. P. prudhoei is hermaphrodites with both male and female reproductive systems functional at the same time, and reproduce by internal fertilization.
    Keywords: Biology ; Fisheries ; Pseudoceros prudhoei
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    Identification of native fish in Hamedan province (Gamaciyab) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25079 | 18721 | 2018-08-22 06:43:41 | 25079 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This study was carried out from 2001 to 2002 in Gamasyab River of Hamadan province of Iran in order to identification of indigenous species of this water resource. The main objective of this study was to fish identification for scientific purposes, geographical distribution pattern, preparation atlas of the fishes, introduction of commercial species and awareness on ecological situation of their natural habitats. Also providing necessary information for conservation and sustainable use of these valuable stocks and minimizing environmental impacts of unsecured utilization. Sampling were carried out by selecting 15 sampling stations through the river and fishing by electro fisher and cast net. Identification accomplished by valid identification keys in species level. Photography and slides provides and 10 pieces of each species tagged by unique codes for preservation in national museum. 50 morphometric and meristic characteristics studied for scientific identification.. Indigenous fishes belong to 14 species, 10 genus, 3 family and 2 order. Indeed 2 exotic species belong to Cyprinidae and Salmonidae families recognized. From these indigenous species, 11 belong to Cyprinidae family and only one species belong to Sisoridae family.
    Keywords: Biology ; Iran ; Hamadan ; Gamasyab River ; Indigenous species ; Identification ; Fish ; Sampling ; Morphometric ; Cyprinidae ; Salmonidae ; Sisoridae
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    Effect of artemia density on cyst yields of fertilized ponds (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25088 | 18721 | 2018-08-22 06:46:47 | 25088 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: One of the effective factors in cyst production on Artemia culture ponds is the number of oviparous females and density of biomass in view of adult's weight per liter of ponds water. In this study, the effect of oviparous female's abundance (Reproductive Females lit^-1) on daily cyst yields with using the ANOVA and correlation Analytical method were assayed. The result indicated that, with presence the small number of oviparous females (less than five Ind lit^-1) and Artemia density (between 0/1 to 0/2g/ lit) on culture ponds, the cyst yields at first sixty days culture period (160 Kg.dw/ha) were more than yields that harvested at three months later (47 Kg.dw/ha) and on the second three months of cultural period despite of presence the larger number of oviparous females (more than twenty Ind/lit) and presence the same Artemia population density, the daily cyst yields was declined.(Sig=0.000).
    Keywords: Biology ; Iran ; Artemia ; Density ; Cyst ; Fertilized pond ; Females ; Biomass ; Weight ; ANOVA
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    Comprehensive study on probability of controlling Caspian Sea invasive ctenophora (Mnemiopsis leidyi) (Activity 2: Investigation on Mnemiopsis leidyi diet in the Caspian Sea) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25091 | 18721 | 2018-08-22 07:12:26 | 25091 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Mnemiopsis leidyi is one the species of comb-jelly. It belongs to the Ctenophore phylum and Lobate order. It is a purely marine, free-swimming animal with a transparent jelly-like walnut-shaped body, which was transported from the Black Sea into Caspian possibly at the end of 1990s. This species is food rival of pelagic fish. It has been reported that the ctenophore caused the dramatic decrease of zooplankton and pelagic fish stock in the Black Sea. In this study, sampling on Mnemiopsis leidyi, zooplankton, phytoplankton was conducted from December 2003 to March 2005, from a total of 11 stations located along four transects (Anzali, Khazarabad, Tourkman and Gorgan Bay) by METU net in the Iranian coasts of the Caspian Sea. Clupeonella catch was done by light fishing with fishing vessel in Anzali region. The results showed that the biomass of M.leidyi had some fluctuation in different seasons and its maximum biomass was recorded in summer with a figure of 524.4±156.2 g.m^2 Tourkman region and the minimum biomass was observed in winter with a figure of 5.15±1.81 g.m^2 in Anzali region. Study of stomach of Mnemiopsis showed, that Acartia (belonged of Copepoda with 66 %), lamlibranchia (13 %) has the highest frequency and the lowest were Balanus nauplii, Rotatoria, Tintinnopsis and Podon polyphemoides. The maximum mean abundance of zooplankton was 29368±24318 n.m3 in winter (Anzali region) and the minimum was recorded 4170±5014 n.m3 in summer (Tourkman region). Copepoda was dominant zooplankton in the Iranian coasts of the Caspian Sea. Chrysophyta with 150000000±440000000 n.m3 and Euglenophyta with 260000±521000 n.m3 had the highest and the lowest frequency respectively in the Caspian Sea. The main foods of Clupeonella were Acartia with 80 %. The survey of stomach of Clupeonella showed that the feeding of these species was not good and the highest fullness index was recorded 220.95±314.67. These results showed, the main food item of Ctenophora and Clupeonella were Copepoda (Acartia). It seems, the impact of Mnemiopsis leidyi feeding has been the important factor in declining zooplankton populations, Clupeonella stocks, and also increasing of phytoplankton abundance.
    Keywords: Biology ; Iran ; Caspian Sea ; Gorgan Bay ; Bandar Tourkman ; Khazarabad ; Anzali ; Ctenophora ; Mnemiopsis leidyi ; Diet ; Species ; Phylum ; Pelagic fishes ; Sampling ; Zooplankton ; Phytoplankton ; Acartia ; Ctenophora ; Population ; Abundance
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    Study on feasibility of rearing integrated native fish of Shizotorax zarudnyi with chianies carp on Sistan area (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25254 | 18721 | 2018-09-07 07:13:59 | 25254 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Present study carried out in 6 earthen ponds (3500 m^2 each) at the Zahak fish propagation and cultivation center. In July 2005 ponds were prepared and stocked with 1100 fish specimens with 40 g average weight. Stocking normative in two ponds (1 and 4) were 220 common carp, 110 grass carp, 715 silver carp, and 55 big head specimens. In the next two ponds (5 and 6) common carp reduced to 50% and Shizotorax zarudnyi was replaced. In the rest two ponds (7 and 8) common carp reduced to zero and totally replaced with Shizotorax zarudnyi. Manure and inorganic fertilizer were used during cultivation period. Carp was fed by concentrated food and grass carp was fed by alfalfa. At the end of the cultivation period (16 months) mean weights of common carp, grass carp, silver carp, big head, and Shizotorax zarudnyi reached 1201 g, 1572 g, 313 g, 486 g, 531 g respectively. Mean daily growth were 2.41 g, 3.19 g, 0.56 g, 0.92 g, 1 g respectively. Survival rate during cultivation period were 97.57%, 68.3%, 94%, 85.45%, 46.85% respectively. Biological parameters were recorded once a month. Other chemical and physical parameters such as air and water temperatures, oxygen, pH, twice per day and factors such as nitrite, nitrate, Ammoniac, transparency, salinity and phosphate were measured and recorded once per week. During cultivation period average pH was 7-10.34, water temperature was 0.5-33 °C, transparency was 12-115cm, salinity was 1.1-5 mg/l, oxygen was 2-10 mg/l, nitrite was 0.86-0.16 mg/l, nitrate was 0.06- 2.21 mg/l, and phosphate was 0.1-1.8 mg/l. Average total yields in the ponds 1 and 4 with standard normative for the region were 554, and 623 kg. In the ponds 5 and 6 with 50% Shizotorax zarudnyi were 514 and 518 kg, and in the ponds 7 and 8 with total Shizotorax zarudnyi replacement were 533 and 386 kg.
    Keywords: Aquaculture ; Iran ; Sistan region ; Fish culture ; Poly-culture ; Shizotorax zarudnyi ; Earthen ponds ; Specimen ; Common carp ; Grass carp ; Silver Carp ; Fertilizer ; Survival rate ; Transparency ; Salinity ; Temperature ; Nitrite ; Nitrate ; Ammoniac ; Tehran ; Iran
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    Population genetic structure of Neogobius caspius (Eichwald, 1831) in the east and central of the south Caspian Sea using microsatellite markers (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25258 | 18721 | 2018-09-07 07:37:38 | 25258 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Neogobius caspius is a small benthic fish that is native to the Caspian Sea. The importance of this fish is because of it is role as a main food resource of the Sturgeon fish. The genetic diversity of N.caspius populations in the Caspian Sea was studied using microsatellite technique. In the study 95 Samples of Neogobius caspius from two regions (Torkman port and Chalus) in south Caspian Sea were collected. DNA was extracted using 12 pairs of microstellite primers polymerase chain Reaction (PCR) was conducted. DNA Bands were analyzed using uvdoct and GenAlEx software package. Out of 12 microsatellite primers, 11 loci were produced, in which 5 of them were polymorphic, 6 monomorph and one of them showed smear. Average of observed and expected hetrozygosity was 0.798 and 0/843 respectively. Significant genetic differences between 2 regions was observed (P〈0.01). Deviations from hardy- Weinberg equilibrium were in all cases. These results indicate that at least, 2 population of Neogobius caspius exist in the south Caspian Sea.
    Keywords: Biology ; Iran ; Caspian Sea ; Torkman port ; Chalus ; Microsatellite ; Genetic ; Population ; Neogobius caspius ; Benthic ; Native ; Sturgeon ; Diversity ; Samples ; PCR
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    Study on inheritance of microsatellite markers in hybrid of chalcaburnus chalcoides and vimba vimba persa in southern part of Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25260 | 18721 | 2018-09-07 07:24:11 | 25260 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: In order to study the Inheritance of Microsatellite Markers in the hybrid of Chalcalburnus chalcoides and Vimba vimba persa in the Southern part of the Caspian Sea, hybridization between the species was carried out during spring (2009) in Shahid Ansari reproduction and culture workstation. 30 samples from the produced larva and also small piece of dorsal and pectoral fin of their parents were collected and stored in pure ethanol (96%) and transferred to the biotechnology of the Caspian Ecology Academy. DNA was extracted from fin samples of the parents, whilst the whole F1 progeny was used to isolate the DNA by Phenol-chloroform method. Quantity and quality of extracted DNA was determined using 1% Agarose electrophorus. DNA was used for PCR ampilification of 10 microsatellite loci. The amplifications were run on 6% Polyacrylamid electrophorus. The present study showed that 7 out of 10 markers were polymorphic and variated between the parents. Goodness of fit to the Mendelian inheritance ratio was determined by X2 analysis. 4 primers were segregrated according to Mendelian expectations at a significance level of P 0.05. Based on UPGMA dendrogram (Nei, 1978) two different clusters were obtained in which Chalcalburnus chalcoides and hybrid were in the same cluster but Vimba vimba persa was in the other. It seems that Vimba vimba persa is a joint ancestor for these two. High heterozygosity presence in parents and their F1 shows the high potential of genetic variety in Chalcalburnus chalcoides and Vimba vimba persa which from the aquaculture management and conservation view is important and considering the high genetic variety in their hybrid, the hybrid has the sufficient potential for race improvement.
    Keywords: Biology ; Iran ; Caspian Sea ; Chalcalburnus chalcoides ; Vimba vimba persa ; Hybrid ; Microsatellite ; Hybridization ; Culture ; Samples ; Larvae ; DNA ; Inheritance ratio ; Heterozygosity
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    Global population structure of the great sturgeon (Huso huso) in the southern part of the Caspian Sea with emphasis on the Golestan Province (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25261 | 18721 | 2018-09-07 07:21:13 | 25261 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Report of the project entitled "determination of the global population structure of the great sturgeon (Huso huso) with emphasis on the Golestan Province" is in front of your attention. Outcome is the results of study on the samples collected from the total catch in the landing sites namely Bandar-e-Anzali, Babolsar, and Ashooradeh, in the three Northern provinces. 224 specimens were collected according to the sampling manual during the fishing seasons in 2005 and 2006. Three methods were applied in this study to find out the population structure of the great sturgeon in the southern Caspian Sea. These were as follows: Morpho-metric meristic Population dynamics Population genetics (microsatellite loci) Results indicate that the range of different age groups of the great sturgeon in the Golestan Province is larger than the other two provinces. Results show that the older age groups are found in the samples from the Golestan Province rather than the other two provinces. Results demonstrate that the sample size for the two methods (morpho-metric meristic and population dynamics) is not enough. In order to obtain precise results with less uncertainty one needs to collect more specimens. Therefore, according to the few number of the great sturgeon in the total sturgeon landing, it is recommended that researchers apply methods other than the two methods which are mentioned above in order to make sure that the results are most reliable. Population genetics methods including microsatellite loci are among the methods which could produce good results with minimum bias in this regard. Results indicate that there are two distinguishable populations of the great sturgeon (Huso huso) in the southern part of the Caspian Sea off the shores of the three Northern provinces. Results also show that these two populations are scattered in the two east end (Golestan Province) and west end (Guilan Province) of the southern Caspian Sea. The two populations have overlapped each other in the Mazandaran Province.
    Keywords: Biology ; Iran ; Caspian Sea ; Golestan Province ; Guilan Province ; Mazandaran Province ; Babolsar ; Bandar-e-Anzali ; Population ; Sturgeon ; Huso huso ; Sampling ; Morpho-metric ; Samples ; Meristic ; Genetic ; Microsatellite ; Specimens
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    Using of benthic indices and GIS maps to determination of biotic health of Khure- Mussa (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25267 | 18721 | 2018-09-07 08:20:55 | 25267 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Without industrial and urban development and their effects on marine ecosystem, due to high biotic potential, Khure-Mussa would be one of the productive coastal areas. Because of sensitivity and vulnerability to environmental changes this area are classified in sensitive ecologic area. This study was conducted to evaluation ecological health status in Khure-Mussa in the Northwest of Persian Gulf by using benthic indices. Twenty creeks in Khure-Mussa area were set up for study and sampling in four seasons from winter 2008 to autumn 2009. Monthly water sampling for physico-chemical parameters was done by bottle sampler and seasonal samples of surface sediment were collected by Peterson grab sampler (0.125m^2) .At each station three samples for macrobenthic study and one sample for grain size analysis and organic matter determination were obtain. Totally, 187589 macrobenthic individual were collected and separated. Among identified groups, Polychaetes with total number108906, Crustacean with total number 46561, Bivalve with total number 14156 and seapen with total number 9841 were the dominant groups and were included 58, 25, 8 and 5 percentage of total number respectively. The highest abundance in the spring and the lowest abundance in the summer were observed. According to mean abundance, Somaily creek and Khure-mussa (3) station showed the highest and the lowest abundance respectively. Seapen is included more than 90 % of total biomass. The range of Shannon diversity index was (1.95-3.58) which the minimum and the maximum values were found in Shipping and Patil station respectively. Significance benthic biological parameters (abundance and richness) in different creeks explained the variation of benthic community structure in studied creeks. In addition, seasonal significant differences were found in these parameters due to seasonal changes in benthic animal s succession. The results of grain size analysis showed that the range of siltclay was (63.6-95.59). According to AMBI index values, all of the studied creeks are classified in slightly pollution level and disturbed condition except for Petrochemical station that placed in moderate pollution level. The BOPA index values in all stations showed more than threshold level (0.19) that means bad and weak ecological condition. The highest and the lowest ITI index values were observed in Oil exporting port station (64.92) and Ghazaleh creek (29.53) respectively. The others were grouped in 30-60 range that means moderate pollution level and disturbed ecological status. The values of ABC (Abundance Biomass Curve) that explained the relation between macrobenthic biomass and abundance, were calculated near zero from 0.1 to -0.1 in all studied creeks that show moderate condition for living animals. In general the studied creeks showed pollution potential and benthic biological parameters indicated downward trend in ecological quality and health status.
    Keywords: Biology ; Ecology ; Iran ; Persian Gulf ; Khure-Mussa ; Ecological health ; Benthic animals ; Biotic indices ; GIS ; Marine ; Ecosystem ; Sampling ; Macrobenthic ; AMBI ; BOPA ; Pollution ; Biomass ; Abundance ; Parameters
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    Identification and density determination of juvenile fishes in Khouzestan coastal waters (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25266 | 18721 | 2018-09-07 07:35:16 | 25266 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Aquatic animals possess specific biological characteristics and lifecycle. Without knowing about their biology and complete lifecycle, planning about their conservation cannot be effective. Following the previous studies on fish larvae in Khuzestan coastal waters this study was done to determination catch composition and density of juvenile fishes in Khuzestan coastal waters during 2007 to 2008. Studied area included two main fisheries ground, Lifeh-Busafe in west and Bahrekan in east coasts. Monthly random sampling was carried out by using shrimp trawl net with Akhtar vessel. Juvenile fish's means the fishes that completed their larval cycle and are look like their parents but have not been matured yet. The catch mean, biomass, CPUA and the stock size of different species were estimated by using Swept Area Method. Totally 101485 caught fishes included 38 families and 63 species. The most abundant trawled fishes were Thryssa vitriostris Leiognathus bindus Ilisha melastoma Penahia macrophthalmus and Johnius belangerii .These five species contain about 80 %, the species Arius dussumieri and Cynoglossus arel 4.5% and 3.2 % respectively and the others 12% of total caught fishes. In the east coasts the species L. bindus I. melastoma T. vitriostris P. macrophthalmus and in the west coasts the species P. macrophthalmus T. vitriostris J. belangerii and I.melastoma were the most abundant. Among 63 identified fish species , juvenile fishes of 23 species were observed which contain 26.4 % of total caught fishes .the fishes Arius dussumieri and Acantopagrus latus 100%, Liza subviridis, Leiognathus lineolatus and Triacantuhus biaculeatus more than 80 % and Pomadasys stridens Lagocephalus inermis Saurida tumbil and Thryssa vitrirostris more than 50 % were observed as juvenile stage. The most abundant juvenile fishes were T. vitrirostris L. bindus and Arius dussumieri respectively. The maximum (1852 kg/km^2) and the minimum (165 kg/km^2) values of CPUA were estimated in west coasts in July and December respectively. The maximum (2677 kg/km^2) and the minimum (153 kg/km^2) values of CPUA were estimated in east coasts in June and August respectively. The biomass showed a distinct peak in June and July in east and west coasts respectively. The highest diversity index in east (2/22) and west (2.36) coasts were obtained in July. In present study the number of identified fishes is more than previous studies. Juvenile fishes spend their sensitive period of lifecycle in Khuzestan coastal waters. After growing in late spring, fish larvae enter to the juvenile stage and select this productive area as nursery ground. Increasing of juvenile fishes in July is accordance with their reproduction season in studied area. The east coasts are deeper than the west and there are some differences in hydrological characteristics due to discharging of the rivers Bahmanshir and Arvandrood in west coasts. The peaks of abundance in east and west coasts were observed with a clear time interval. The higher CPUA in east coasts can be caused due to gradual migration of juvenile fishes from west to east during cold season.
    Keywords: Aquaculture ; Biology ; Iran ; Persian Gulf ; Khuzestan coastal waters ; Bahrekan ; Bahmanshir River ; Arvandrood River ; Juvenile fishes ; Bottom trawl ; Catch composition ; Biomass ; Aquatic ; Larvae ; Density ; Sampling ; CPUA ; Species ; L. bindus ; I. melastoma ; T. vitriostris ; P. macrophthalmus ; J. belangerii ; Migration ; Coastal waters ; Identification
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    Reproduction biology of Astacus leptodactylus eichwaldi in the Iranian coastal water of the Caspian Sea (Bandar Anzali) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25270 | 18721 | 2018-09-07 07:54:06 | 25270 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Sampling of Astacus leptodactylusc were conducted in order to determination of biometrical and biological parameters suchas length, weight, sex ratio, fecundity and natural reproduction time. Two transect were selected at 49 34 and 49 36 geographical position on east Caspian Sea near to Anzali city. Metallic folding trap with Silurus glanis as attractive diet were used to catch Astacus leptodactylusc at each line the traps were set on depth of 35, 45, 55 and 65 (5 trap at each depth). Random sampling from each depth on tow lin for one year were conducted and the biometry performed on catched Astacus leptodactylusc where their sext uality and their ration were determined for eacd month , season and whole year. Absolute fecundity determined by cooking Astacus leptodactylusc , taking out the ovary weighing and counting them .Working fecundity assesed by separating eggs from their swiming leges while enomerate all egg . Complete randomized test of ANOVA for analysing the data were employed. The results showed average length and welght were calculated 122/07±1/74mm and 57/96±1/81gr respectively. Average absolute fecundity was 310/22 ±10/72 eggs , average working fecundity was 251/84±8/84 eggs, Average ovary weight was determined 4.31±0.619 gr and average number of eggs in one gr was 74/52±1/53 eggs. The sextual ratio in all year long was 1:1.32. The reproduction season is about seven month from Feburary to July and the moulting of males occurs two times each year. One of time is at May and the other is in September. The female molt thtina as the male start for second time.
    Keywords: Biology ; Iran ; Caspian Sea ; Bandar Anzali ; Biology ; Astacus leptodactylus eichwaldi ; Sex ratio ; Fecundity ; Silurus glanis ; ANOVA ; Female ; Male ; Coastal water
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    The effect of different dietary and bed treatments on earthworm (Eisenia fetida) for aquaculture (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25272 | 18721 | 2018-09-07 08:25:12 | 25272 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This experiment conducted to evaluate the effect of different dietary and bed treatments on earthworm (Eisenia fetida) for aquaculture. Concurrent with initial proliferation of earthworms, a pilot experiment also performed. In pilot experiment that conducted to become familiar with specialty and requirements of Eisenia fetida earthworms, lasted 4 months. The pilot treatments were including: 1) cow manure with covering its surface (control group), 2) cow manure (C.M.) without covering its surface, 3) C.M.+ 5% wind sand, 4) C.M.+30% saw dust, 5) urban compost, 6) C.M.+20% alfalfa meal, 7) C.M.+ 20% wheat bran and 8) C.M.+20% rice bran. After initial proliferation of earthworms and performing a pilot experiment, two trials designed. The first trial performed in outdoor environment, inside the plots dugged in the earh, with six treatments. The treatments were including: 1) cow manure(control group), 2) cow manure (C.M.) with vertical ventilation, 3) C.M.+ 30% saw dust (S.D.), 4) C.M.+30% S.D. with vertical ventilation, 5) urban compost+20% C.M. and 6) urban compost+40% C.M. The experiment lasted 6 months. In the end of trial in every plot, from 4 points, sampling for number of mature and immature earthworms, cocoons and the weight of earthworms were measured. The second trial performed in greenhouse by using wooden boxes. In this trial the treatments were including: 1) C.M. as control group, 2) C.M.+30% S.D., 3) C.M.+30% S.D.+ 10% alfalfa meal, 4) C.M.+30% S.D.+10% wheat bran, 5) C.M.+30% S.D.+10% rice bran, 6) C.M.+30% S.D.+10% soil, 7) C.M.+30% S.D.+5% fine sand, 8) C.M.+ 30% S.D.+1% lime powder and 9) C.M.+30% S.D.+200 ppm ammonium nitrate. Every treatment performed in 3 replicates. The earthworms reared in controlled condition for moisture and temperature, for about 4 months. The pH of treatments measured monthly by paper pH meter and by method of solution extraction. In the end of trial the number of mature and immature worms, cocoons and the weight of earthworms in every box were measured and the average weight of every earthworm calculated. The results of pilot experiment showed that covering surface the bed is necessary for maintaining moisture and darkness for earthworms. In wind sand treatment, the bed had sticky and compacted statues and appeared that the earthworms moved and activated difficultly. The saw dust treatment had a wet- spongy environment and the earthworms distributed well in it and their size was larger than control. Urban compost treatment unlike its suitable pH (equal 8), hardly accepted by the earthworms and they stayed in the corner of bed for more than one month. The wheat bran and rice bran treatments, both had sticky texture at the beginning but by spending time, their beds improved. The results of first trial showed the Eisenia fetida earthworms can survive and growth in outdoor environment even in cold season of winter. In this trial the highest total number of eartworms and cocoons and the total weight of earthworms was belong to control group, that had significant difference with the treatments C.M. + 30% S.D., urban compost + 20% C.M. and urban compost + 40% C.M. (p〈0.05). The performing of ventilation in experimental treatments had not significant effect on the number of mature earthworms, total number of earthworms and their total weight compare to control group. The urban compost was not suitable for rearing Eisenia fetida, although addition of 20 and 40 % of cow manure could improve it for usage by the earthworms. The results of second trial showed that, addition of alfalfa powder, wheat bran, rice bran and lime powder to cow manure had not significant effect on the total number and total weight of earthworms compare to control group. The usage of soil in treatment C.M. + 30% S.D. + 10% soil caused significant decrease in number of mature earthworms compare to control group (p〈0.05). The treatments including addition of saw dust and saw dust + fine sand to cow manure increased the number of mature earthworms, cocoons and total weight of earthworms compare to control group significantly (p〈0.05) and treatment of ammonium nitrate caused significant increase in total weight of earthworms (p〈0.05) .
    Keywords: Aquaculture ; Iran ; Diet ; Treatment ; Earthworm ; Eisenia fetida ; Aquaculture ; Dietary ; Ammonium ; Nitrate ; Temperature ; pH ; Mature ; Immature ; Growth
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    Ecological study on rearing shrimp ponds (Litopenaeus vannemei) in Choebdeh Abadan (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25276 | 18721 | 2018-09-07 07:59:49 | 25276 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Following to introduce Vannamei shrimp (Litopenaeus vannamei) into Iran aquaculture industries by fisheries organization its postlarvae were successfully reared in ponds in Bushehr and Choebdeh in Khuzestan. Due to activity of Shrimp site and importance of input and output water quality, this study was done in 11 stations including: two stations in Bahmanshir river, two station in C4 and C5 irrigation canals, six staions in active farms along C4 cannel (Yonesi, Mosavei and Salman Zadeh), and C5 cannel (Mohamadi, Khairi and Ashraf Por) and one staion in output draing were selected. Sampling were carried out from May2009 befor beginning stocking to harvest time in October 2009. Physico- chemical parameters and plankton sampled biweekly and benthic animals and heavy metals were sampled monthly. Some parameters such as pH and temperature by using portable multy parameters and others. Analyzed with standard methods. Heavy metals were measured by plarography method (797 Vamtro). Plankton and benthos samples were studied only in Bahmanshir station. According to results the maximum values for DO (11.1 ppm) PO4 (1.86 ppm) NO3 (8.4 ppm) and TSS (4992 ppm), pH (8.4) NO2 (0.18 ppm) BOD5 (9.06 ppm) were measured. In comparison to aquaculture water quality standards except for TSS in river stations others are located in allowable range. We didn’t observed any main changes in water quality from river to output drainage. The results of biotic parameters in river stations showed that diatoms were the most group of phytoplanktons. Zooplankton groups were Copepods, Rotifera and Protozoa. Tintinids and Nematoda larvae were the most abundant zooplankton. Benthic animals were included nine crustacean species and two annelid species. Isopods with 52% were the most and ant group.
    Keywords: Aquaculture ; Ecology ; Iran ; Khuzestan Province ; Abadan ; Choebdeh ; Bahmanshir River ; Vannamei shrimp ; Litopenaeus vannamei ; Water quality ; Abadan aquaculture site ; Rearing ; Shrimp ; Ponds ; Sampling ; Plankton ; Heavy metals ; pH ; Temperature ; Benthos ; Phytoplankton ; Zooplankton ; Copepods ; Nematoda larvae
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    Estimation of MSY on six species of commercially important demersal fishes in the Persian Gulf & Oman Sea (Hormuzgan province) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25274 | 18721 | 2018-09-07 07:40:17 | 25274 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Today, calculation of MSY is one of the necessary fisheries management in control and prevention of the fish population reduction and is obtained with different methods. This study has focused on six species of commercial fish, including Tiger-toothed croaker, Javelin grunter, John`s snapper, Indian spiny turbot, Yellowfin seabream and Silver pomfret. The study was done monthly, from January 2007 to March 2008, in three fish landing regions including: Bandar Lengeh, Bandar Abbas and Qeshm Island (Slakh, Basydu and Chahoshrqy). Total 5163 Silver pomfret (Pompus argenteus), 1766 Javelin grunter (Pomadasys kaakan), 2151 John`s snapper (Lutjanus johnii), 3280 Tiger-toothed croaker (Otolithes ruber), 1628 Indian spiny turbot (Psettodes erumei) and the number of 759 Yellowfin seabream (Acnthopagrus latus) were assessed and length biometry has been done, monthly. In this study, two methods were used to determine the maximum sustainable yield (MSY): 1- virtual population analysis (Cohort analysis) 2- use of statistics and information that was estimated with two method, catch prediction and biomass (Standing stock). The results showed that in 2007, MSY value was estimated through catch prediction for Silver pomfret, Tigertoothed croaker, Javelin grunter, John`s snapper, Indian spiny turbot and Yellowfin seabream 1354, 1116, 1099.6, 1045.5, 914.5 and 529.5 tons, respectively. Moreover, this estimation have been done through standing stock for Silver pomfret, Tiger-toothed croaker, Javelin grunter, John`s snapper, Indian spiny turbot and Yellowfin seabream 1215, 633, 1304, 878, 1095 and 441 tons, respectively; and through VPA for Silver pomfret, Tiger-toothed croaker, Javelin grunter, John`s snapper, Indian spiny turbot and Yellowfin seabream 1100, 850, 920, 732.5, 1002.3 and 403 tons, respectively. Amount of biomass (Standing Stock) was estimated for Silver pomfret, Tiger-toothed croaker, Javelin grunter, John`s snapper, Indian spiny turbot and Yellowfin seabream 2530, 1172, 1738, 1689, 1470 and 1110 tons, respectively. In general, by assessing the obtained results for the studied species, except the fishing prediction results of the Javelin grunter and Tiger-toothed croaker species, which is less valuable due to the low correlation coefficient, it can be stated that except fishing pressure on John`s snapper and Tiger-toothed croaker, in other species studied, fishing conditions are in optimal situation.
    Keywords: Biology ; Iran ; Persian Gulf ; Oman Sea ; Hormuzgan Province ; Bandar Lengeh ; Bandar Abbas ; Qeshm Island ; Silver pomfret ; Tiger-toothed croaker ; Javelin grunter ; John `s snapper ; Indian spiny turbot ; Yellowfin seabream ; Biomass ; MSY ; Species ; Demersal fishes
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    Study on biological factors for artificial reefs in Hormozgan Province (Bandar Lengeh area) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25290 | 18721 | 2018-09-07 07:55:23 | 25290 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This study was carried out for growth of organism’s survey on artificial reef from 2004-2006 after establishment in Hormozgan offshore waters (Bandar Lengeh area). Also during this study we were examined some biological factors including: phytoplankton, zooplankton, ichthyoplankton and benthos from spring 2006 to winter 2006. In this study 43 phytoplankton genus were identified, 28 Bacillariophyceae (Diatom), 11 Dinophycea, 3 Cyanophycea and 1 Euglenaphycea. Bacillariophycea were dominant, Cyanophycea were high density observed in summer and Euglenaphycea was only observed in winter. The most abundant genus of diatoms were: Rhizosolenia, Navicula, Nitzschia, Coscinodiscus. Ceratium, Prorocentrum and Protoperidinium belong to Dinophycea, Oscillatoria belong to Cyanophycea were more than other genus. Copepoda and Nauplius were the most zooplankton and other group were respectively: Oikopleura, Polychaeta and Chaetognatha. The highest density of zooplankton was seen in winter. The statical study between different stations didn't show any significant (p〉0.05). 6 ichthyoplankton families were seen and identified. These families were Clupeidae, Gobbidae, Callionymidae, Sparidae, Engraulidae and Scianidae. The high density were respectively in summer and spring. The highest annual mean belong to Gobbidae family. Benthos groups were including: Crustacea, Mollusca, Polychaete, Nematoda, Nemertin, Foraminifera, Opiouridae and Echiura. Annual high density of Crustacea were observed in spring season, Polychaete and Mollusca were observed respectively in summer and winter. Organisms which growth on the artifical reef were consist of 12 group. These Organism were Barnacle belong to Crustacea with one genus (Megabalanus tintinnabulum), Sponga with 5 families and Tunicate. The highest density was in 2005. We were seen increasing in size in some organisms such as Crab, Polychaetes, Bivalvia and Fish Larvae. Statical study didn't show any significant between organisms in different parts of artificial reef (top, mid, bottom) and kind of artificial reef had no effect on growth of organisms but Barnacles had high density on mixed artificial reef.
    Keywords: Biology ; Ecology ; Iran ; Persian Gulf ; Hormozgan Province ; Bandar Lengeh ; Artificial reef ; Plankton ; Benthos ; Growth ; Organisms ; Phytoplankton ; Zooplankton ; Ichthyoplankton ; Bacillariophyceae ; Diatom ; Dinophycea ; Cyanophycea ; Euglenaphycea ; Rhizosolenia ; Navicula ; Nitzschia ; Coscinodiscus
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  • 81
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    Identification of Kerman Fishes (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25301 | 18721 | 2018-09-08 16:29:41 | 25301 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This study was performed as a research program in Agricultural Research center of Kerman since 2000. During one year identification of fish in qanat basins of Markazi desert and Sirjan desert was carried out. In this study Electroshoker and salik net with o.5 and 1 cm network and padel net with 1cm were used to catch the fishes from 73 series of qanat. Parameters estimated in qanats (pH, Weather Temperature, Oxygen , Ec,...) Specified in sum 767 from fishes which were in 2 order 3 family and 3 species as: 1-Order: Cypriniformes. Family: Cyprinidaea Species: Capoeta damascina (Valenciennes, 1849) family: Balitoridaeb species: Schistura sargadensis (Nikolskii, 1900) 2-Order: Cyprinodontiforms Family: Poecilidaea Species: Gambusia affinis (Baird and Girard, 1853)
    Keywords: Biology ; Iran ; Kerman ; Sirjan ; Qanats ; Fish ; Capoeta damascina ; Gambusia affinis ; Schistura sargadensis ; pH ; Weather Temperature ; Oxygen ; Species ; Cypriniformes ; Cyprinidaea ; Balitoridaeb ; Cyprinodontiforms ; Poecilidaea ; Identification
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  • 82
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    The study of annual cycle of gonads developments and sex steroids of gray mullet, Mugil cephalus in Golestan Province (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25319 | 18721 | 2018-09-11 09:09:09 | 25319 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Grey mullet, Mugil cephalus is one of the most famous and valuable fish on the fisheries and aquaculture industry. This species is the most suitable to introducing the southern, northern and central regions of our country due to high resistant to large variation of water salinity and temperature. On the monthly sampling period biometric data including total length and total weight recorded and length and weight classes for every sex were determined. To understanding of physiological functions and sexual development of reproductive organs, this investigation carried out with Grey mullet imported from Hong Kong by 1993. Findings of this research can be used for management of artificial breeding of this species. Daily variation of pond water temperature was recorded. Sex steroid hormones and other plasma metabolites such as Ca^2+, cholesterol, triglycerides and total proteins were measured by blood sampling. Gonad samples were prepared in order to histological studies. Oocyte growth was studied by measurement of egg diameters. Measured values of cholesterol, triglycerides of blood plasma revealed that these metabolites have seasonal variation and severely deposited on the summer and mobilized on the spawning seasons. Findings of this research suggested that egg diameters, values of sex steroid hormones (estradiol and progesterone) and Calcium concentrations of fish blood plasma shortening increased a long with day length shortening.
    Keywords: Aquaculture ; Iran ; Golestan province ; Mugil cephalus ; Sex steroid hormones ; Plasma metabolites ; Gonads ; Gray mullet ; Mugil cephalus ; Fisheries ; Aquaculture ; Species ; Salinity ; Temperature ; Sampling ; Estradiol ; Progesterone ; Calcium
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  • 83
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    Survey of diversity, distribution, abundance and biomass of macrobenthic fauna in the southern Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25329 | 18721 | 2018-09-13 12:35:03 | 25329 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This project were investigated seasonally from march 2008 up to one year, in southern Caspian Sea iranian coast /Sampling was carried out at 8 stations of between 5 and 100 m depth and taken in triple with a 0/1m² van Veen grab/Total twenty four species and one family and one order were identified, presenting low dominance and frequency/ polychaeties 56%,oligochaetes 39% bivalves 〈1% amphipodes1% Cumacea and diptera and cirrepedia %1 of whole macrobenthos compositions/ Polychaetes and oligochaeta dominated, representing 95% of the whole fauna/Average of species were 11/8 species per grab/max 21 species / grab were found in Tonkabon stations at 100 meter depths and minmum 4 species/grab in 20 m in Bandar anzali /Maxmiun density were 4783 n/m^2 in spring and minmum 3177 n/m^2 in winter/Highest density were recorded in Amirabad stations and lowest in nooshar/ Maximum density showed in east, mimun in middle area of sea The biomass of benthos adventages in west/Average biomass of whole area were 6/932 gr/m^2/Mean total organic matter were 3/11±1/1 and silt- clay 62/92±6/12precent and Mean dissolve oxygen and salinity were 8/3±0/15 mg/l and salinity 12/2 ±0/4 ppm and water tempreture were 14/9±0/18ْC/ Mean grain size, fine sand, very fine sand, silt and clay contents were among the main factors related to the patterns of macrofaunal distribution, density and diversity and dominance of trophic groups.
    Keywords: Ecology ; Iran ; Caspian Sea ; Tonkabon ; Bandar Anzali ; Macrobenthos ; Disturbiotion ; Diversity ; Productions ; Survey ; Diversity ; Abundance ; Biomass ; Macrobenthic Fauna ; Sampling ; Species ; Polychaeties ; Oligochaetes ; Bivalves ; Dissolved oxygen ; Salinity ; Temperature
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  • 84
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    Hydrology, hydrobiology and environmental pollution study of the southern of Caspian Sea basin (10-100 meter depth) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25335 | 18721 | 2018-09-13 12:35:23 | 25335 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This study was conducted to study of Hydrology, Hydrobiology and Environmental pollution in the southern of the Caspian Sea. 33 stations were selected from Astara (in the west) to Hassan gholi (in the east). In each station, sampling carried out at suface, 10, 20, 50 and 100m depths. Sampling was performed during four seasons in 2004. Sampling stations also divided into three regions, including west (from Astara to Ramsar), middle (from Ramsar to Sari) and east (from Amirabad to Hassengholi). The results showed that maximum of water temperature was 24 ?C at summer in the eastern region while the minimum of water temperature in winter season has been 9.7 ?c in the western region. The average of dissolved oxygen concontration in spring, summer, autumn and winter season were 6.9 , 6.6, 8.2 and 6.7 mg/l , respectively. The maximum value of salinity was observed in spring (13.55 ppt) and minmum value in winter (8.24 ppt). The average of total nitrogen concentration were season was 711, 884, 583 and 669 µg/l in spring, summer, autumn and winter repectively. The average of total phosphorus concentration was 27.9, 37.1 and 42.6 µg/l in spring, summer, autumn and winter, respectively. The average of dissolved silica concentration has varied from 167 to 923.6 µg/l. The five phyla of phytoplankton have identified. The most species diversity was observed in winter (70 species) but the low species diversity has obtained in autumn. The most phytoplankton population was in spring and summer seasons. The cell abundance of phytoplankton in different seasons was fluctuated from 3914*103 to 17337*103 ind/m^3. In addition, the phytoplankton biomas was varied 11.4 to 30.7 mg/l. Result showed that distribution of phytoplankton are varied at different areas, layer, seasons and stations. The average of cell abundance of zooplanktons was varied from 2023 to 12364 and/m^3 at different seasons. The mean of bioman varied from 9.40 to 43.48 mg/l. In this study, 17 species and one class of macrobenthos were identified. The frequency of Annelida and Biovalvia was higher than all macrobenthos. The average of macrobenthos was 1218±851 ind/m^2 and their biomass was 14±15 g/l. The average of PAHs concentration were 0.038, 0.028 and 0.21 mg/l in spring , summer and winter, respectively. The decending trend of heavy metal concentration were Fe〉Mn〉Zn〉Cr〉Pb〉Co〉Cd〉Cu at different seasons in 2004. The maximum concentrations of organochlorine pesticides (OCPs) components in three regions of sampling zone such as , -BHC (middle), β,-BHC (middle), DDT (east) and dieldrin (east part) were 88.3, 35.0, 74.3 & 79.1 g/l during spring, summer, fall and winter, respectively.
    Keywords: Ecology ; Iran ; Caspian Sea ; Astara ; Physo-chemical parameters ; Plankton ; Benthos ; Enviromental Pollutions ; Hydrology ; Hydrobiology ; Sampling ; Temperature ; Dissolved oxygen ; Dissolved silica ; Species ; Phytoplankton ; Biomass
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  • 85
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    Identification of distribution of Macrobrachium nipponense in freshwater ecosystems and coastal waters of Golestan Province, Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25334 | 18721 | 2018-09-12 09:56:40 | 25334 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Distribution of freshwater shrimp (Macrobrachium nipponense) in the freshwater ecosystems such as Alagol, Almagol, Ajigol lagoons, Gomishan lagoon and Gorganrud River. Sampling were done from may 2009 to my 2010 parameters such as, length frequency, growth rate, sampling, feeding, environmental factors and sedimentation were investigated. 3689 females and 8551 males were collected and biometrical parameters were recorded. Total length ranged between 3.2 to 98.7 mm. The parameters b of length-weight relationship in Alagol, Almagol, Ajigol were 3.49, 3.15, 3.45, 3.22 and 3.22, 3.28 for male and female, respectively. It shows in all lagoons for male and female growth is positive Allometric. Spawning period was from May to June. The length first at maturity (total and/or carapace) for three lagoons was 50.5 mm and 133 mm, respectively. The minimum and maximum of egg volume were 0.12 mm3 in Ajigol and 0.07 mm3 Alagol, respectively. The relationship between fecundity and length was found to be linear regression models for Alagol, Ajigol and nonlinear regression models for Almagol lagoon. Result showed that this species is omnivorous. The minimum and maximum temperature was 6.5ْ C in fall and 33ْ C in summer. The lowest and highest fluctuations of pH level in Ajigol and alagol lagoons were 8.2-9.2 and 7.2-9.8. Among three lagoons, the most EC was 5.9 Ms/c in Alagol lagoon. The Gorganrud River shows the maximum EC, 16.7 Ms/c.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Golestan province ; Alagol lagoon ; Almagol lagoon ; Ajigol lagoon ; Freshwater shrimp ; Macrobrachium nipponense ; Wetland ; Biology ; Ecosystems ; Sampling ; Growth ; Eggs ; Identification ; Distribution
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  • 86
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    The study of possible biotechniques and normative to production some species of Anostraca (Phallocryptus spinosa) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25363 | 18721 | 2018-09-14 07:46:07 | 25363 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This project was carried out in order to determine the hatching conditions and laboratory culture methods of Fairy Shrimps (Phallocryptus spinosa). Physico- chemical factors and phytoplankton of natural habitat of this organism was investigated in Khasellou region around Azarshahr – East Azarbaijan province. The results showed that occurance and growth of fairy shrimps in their habitates were beginning from early Aprill and their survival was decreased with increasing temperature, precipitation and increasing the salinity of their culture medium. Then they disappeared. Their habitat phytoplankton contained 3 phylla and 10 genera, from which green algae enjoyed the greatest density in May. The pool salinity was around 20-17 g/l, when metanauplii was observed in early spring. In the pool containing and without fairy shrimps the oxygen concentration was high (9 mg/l) and low (2.9 mg/l), respectively. In 9 prawn ornamental fish genera, feeding with fairy shrimp showed a significant higher fecundity and hatching percentage compared to manual diets (cow liver, spinach). In all groups fed with fairy shrimp the duration of spawning time were reduced from 15 to 8-10 days and the color was enhanced. The means of cyst number per captured female also, cyst, decapsulated cyst and Naupilli diameters were mesured 142.9±19.0 cysts, 273.2±4.9µ, 242.4±3.8µ and 542.6±27.0 µ, respectively. The hatching rate was increased by reducing temperature and salinity of their culture mediums from 25 to 15 ◦C , and 28 to 18 g/l, respectively. The highest hatching was observed on 5th day at 15 ◦C and 18 g/l (5, 33 %). In addition, low but multiple hatching was observed. Therefore, water temperature and salinity affected hatching rate in this species. The hatching success of Phallocryptus spinosa cysts was better when collecting cysts together with dry sediments of their habitat without separation sensitive cysts. In addition to cold keeping and freezing, the hydration – dehydration method caused more diapause deactivation for hatching induction. The laboratory results revealed that the survival of fairy shrimps were highest at 15 ◦C (42%) and lowest at 25◦C (26%), respectively. Also, their life was inhibited in 30◦C. In different salinities, the mean survival rate were 26% (Salinity= 15 g/l) and 20% (Salinity= 25 g/l), respectively. The mean survival percentage of Phallocryptus spinosa cultured in natural habitat water (64.3%) was higher than aerated tap water (23, 3 %). Also, feeding with Nanochlorepsis caused higher survival rates. On the other hand, Beakers yeast caused higher mortality rates. Therefore, regarding to relatively high sensitivity of these species to physico– chemical and nutritional conditions of their culture medium, it is better to culture these species with expansion of their natural habitat in order to preserve their biodiversity, culture and increase stocking density with regard to environmental considerations .
    Keywords: Aquaculture ; Iran ; East Azarbaijan province ; Fairy Shrimps ; Phallocryptus spinosa ; Hatching ; Culture ; Biotechnique ; Species ; Anostraca ; Phytoplankton ; Growth ; Temperature ; Salinity ; Phylla ; Feeding ; Survival rate ; Mortality ; Biodiversity
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  • 87
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    Determing some important extracted materials, such as: Alginates of sodium & calcium and alginic acid in three species of brown seaweeds (Sargassum illicifolium, Cystoseira indica and Nizimuddinia zanardinii) in coastal area of Sistan and Baluochestan Province (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25376 | 18721 | 2018-09-14 07:34:10 | 25376 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This study was determined some important extracted materials, Such as : Alginates of sodium & calcium and alginic acid in three species of brown seaweeds (Sargassum illicifolium , Cystoseira indica and Nizimuddinia zanardinii) during in different season in 1387 in coastal erea of Sistan and Baluochestan Provience. At First seaweeds sample were colected from sea beach and transferd to lab, so washed in fresh water and dried in the sun. So were under Alkaloid extraction with sodium carbonate (5 Persent), that were obtined fluid after filteration. The sodium alginate fibers extracted from fluid were combined by Alcohol. The calacium alginate fibers were deposited by calacium chlorids (5%) and Alginic acid resulted with chloridric acid (1N). The extraction yield of sodium alginate, calacium alginate and alginic acid in different samples of seaweeds with following Explaination: The presentage we have got, can be said , the present average of sodium alginate at Sargassum in Spring season in three areas (Chabahar, Tang and Pasabandar ) Respectively: 28.4±2 , 28.9±2 , 27.2±1.9 present, the present average of sodium alginate at Cystoseira in Spring in three areas at above mentioned goes in this way: 19.7±1.4, 18.6±1.4, 19.2±1.3 present and the present average of sodium alginate at Nizimuddinia in Spring were 23.4±1.5, 23.8±1.5 , 20.6±1.5 in these three areas. The present average of calacium alginate at Sargassum in Spring season in three areas (Chabahar, Tang and Pasabandar ): 33.7±0.5, 33.7±0.8, 33.1±1 present, the present average of calacium alginate at Cystoseira in Spring in three areas at above mentioned goes in this way: 28.8±1.5, 27.4±1, 27.4±1.5 present and the present average of calacium alginate at Nizimuddinia in Spring were 35.9±2.8, 23.9±1.5 , 35.9±2.8 in these three areas. The present average of Alginic acid at Sargassum in Spring season in three areas (Chabahar, Tang and Pasabandar ): 19.2±1.6 , 25.6±1.7 , 18.4±1.1 present, the present average of Alginic acid at Cystoseira in Spring in three areas at above mentioned goes in this way: 16.8±1.2, 15.8±1.1, 16.4±1 present and the present average of Alginic acid at Nizimuddinia in Spring were 19.2±1.6, 19.6±1.7 , 18.4±1.5 in these three areas. The present average of sodium alginate at Sargassum in Summer season in three areas (Chabahar, Tang and Pasabandar ) Respectively: 20.8±1, 21±1, 27.8±1.9 present, the present average of sodium alginate at Cystoseira in Summer in three areas at above mentioned goes in this way: 21±1.1, 13.2±1, 14.1±0.7 present and the present average of sodium alginate at Nizimuddinia in Summer were 25.3±2.3, 16.7±2.1, 15.3±1.3 in these three areas. The present average of calacium alginate at Sargassum in Summer season in three areas (Chabahar, Tang and Pasabandar): 29.5±2.1 , 29.7±2 , 28.3±2.2 present, the present average of calacium alginate at Cystoseira in Summer in three areas at above mentioned goes in this way: 21±1.1, 20.2±0.9, 20±0.7 present and the present average of calacium alginate at Nizimuddinia in Summer were 25.3±2.3, 29.7±2 , 23.1±1.5 in these three areas. The present average of Alginic acid at Sargassum in Summer season in three areas (Chabahar, Tang and Pasabandar): 19.5±0.7 , 19.7±0.6 , 18.7±0.7 present, the present average of Alginic acid at Cystoseira in Summer in three areas at above mentioned goes in this way: 21±1.1, 11.5±1.4, 11.1±1.5 present and the present average of Alginic acid at Nizimuddinia in Summer were 14.8±1.3, 15.3±1.3 , 14.6±1 in these three areas. The present average of sodium alginate at Sargassum in Autumn season in three areas (Chabahar, Tang and Pasabandar) Respectively: 31.5±4.3, 31.6±4.8, 29.4±4.8 present, the present average of sodium alginate at Cystoseira in Autumn in three areas at above mentioned goes in this way: 23±2.7, 21.4±2.8, 21.4±2.7 present and the present average of sodium alginate at Nizimuddinia in Autumn were 25.8±2.2, 26.4±2.1 , 23.4±1.9 in these three areas. The present average of calacium alginate at Sargassum in Autumn season in three areas (Chabahar, Tang and Pasabandar): 43.3±1.6, 42.8±2.7, 41.7±2.9 present, the present average of calacium alginate at Cystoseira in Autumn in three areas at above mentioned goes in this way: 34.3±5.9, 32.4±5.5, 30.9±5.2 present and the present average of calacium alginate at Nizimuddinia in Autumn were 50±7.1, 50.2±7.5, 45.6±5.2 in these three areas. The present average of Alginic acid at Sargassum in Autumn season in three areas (Chabahar, Tang and Pasabandar): 28±3.8, 28±3.5, 26±3.9 present, the present average of Alginic acid at Cystoseira in Autumn in three areas at above mentioned goes in this way: 20.2±1.8, 19.2±2.1, 18.7±1.6 present and the present average of Alginic acid at Nizimuddinia in Autumn were 25.9±3, 26.1±2.9, 24.6±3.6 in these three areas. The present average of sodium alginate at Sargassum in Winter season in three areas (Chabahar, Tang and Pasabandar) Respectively: 35±1.6, 35.2±1.8 , 33.8±1.9 present, the present average of sodium alginate at Cystoseira in Winter in three areas at above mentioned goes in this way: 24.1±2, 22.9±1.2, 23.3±0.7 present and the present average of sodium alginate at Nizimuddinia in Winter were 28.2±1.3, 29.2±1.7, 26.2±1.8 in these three areas. The present average of calacium alginate at Sargassum in Winter season in three areas (Chabahar, Tang and Pasabandar): 45.6±1.9, 45±2, 44.6±2.5 present, the present average of calacium alginate at Cystoseira in Winter in three areas at above mentioned goes in this way: 38.2±2.8, 35.9±3.4, 35.6±3.5 present and the present average of calacium alginate at Nizimuddinia in Winter were 56.4±3.5, 56±3.5, 51.8±2.5 in these three areas. The present average of Alginic acid at Sargassum in winter season in three areas (Chabahar, Tang and Pasabandar): 31.8±1.2, 32.1±0.9, 30.9±1.2 present, the present average of Alginic acid at Cystoseira in Winter in three areas at above mentioned goes in this way: 21.1±1.2, 20.4±1.4, 19.3±1.4 present and the present average of Alginic acid at Nizimuddinia in Winter were 26.4±2.1, 27±2.2, 24.6±2.2 in these three areas. The more amount of calacium alginate in Autumn can be found at Nizimuddinia in Tang area , wite amount of 50.2 present and the lates amount of Alginic acid at Cystoseira can be found in Summer with 11.1 present. If the Alginic acid become more than 20 present, it can be economical, that it can be showed more than 20 present in Autumn and Winter. According to this results, One way ANOVA showed that average of Alginates were not similar and significant differences ( P〈0.05 ) between species.
    Keywords: Biology ; Iran ; Sistan and Baluochestan Provience ; Chabahar ; Tang ; Alginic acid ; Species ; Brown seaweeds ; Sargassum illicifolium ; Cystoseira indica ; Nizimuddinia zanardinii ; Chloridric acid ; ANOVA ; Sodium ; Calcium ; Seaweeds
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  • 88
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    Ecological study on fisheries important rivers and bays in the south part of the Caspian Sea 2nd phase: Gorgan Bay (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25385 | 18721 | 2018-09-18 05:58:09 | 25385 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: In this study ichthyoplankton, phytoplankton, zooplankton, benthic and hydrochemistry parameters were studied. 9 out of 11 sampling stations were in the Gorgan Bay area and 2 of them were in the mouth of the Bay. Ichthyoplankton survey were carried out in 6 stations and the other surveys such as phytoplankton, etc were done in 11 stations in 2009. Gorgan Bay map and position of sampling sites are given at the end of material and methods section. Biomass and frequency of phytoplankton at different stations, months and seasons were calculated that are presented in Tables 1 to 3. Hydro-chemical parameters such as water temperature, air temperature, visibility, DO, BOD5, pH, Alkalinity (bicarbonate, carbonate and total), Hardness (calcium, magnesium), CL,EC, Salinity, N (nitrate, nitrite, NH_3), Phosphate were measured and results are shown Table 11. Biomass (mgr/m^3) and numbers (ind./m^3) of 47 genus of different phylum of phytoplanktons such as Cyanophyta, Chlorophyta, Pyrophyta, Chrysophyta and Euglenaphyta as well as different phylum of zooplanktons consist of Protozoa, Arthropoda, Rotifera and Cyliophora were estimated. Frequency of phytoplanktons (ind/m^3) was 131.2, 1.6, 65.3, 18.9 and 23.8 million respectively. Furthermore, their biomass was 308.4, 1.9, 1358.9, 295.1 and 124.7 mg/m^3, respectively. The maximum and minimum numbers were observed in Leptolingbaya belonged to Cyanophyta and Diatoms of Chrysophyta 84.3 and 0.025 million (ind/ m^3) respectively. The maximum and minimum biomass was observed in Gyrosigma (Chrysophyta) and Tetradron (Chloropyta), 12317.3 and 0.014 mg/m3 respectively. The average biomass of zooplankton was measured 531.74, 30.77, 225.07, and 96.26 mg/m^3 respectively and the total was attained 883.83 mg/m3 Table 5. The maximum and minimum biomass of all zooplanktons was estimated in November and July, 3446.23 and 6 mg/m^3 respectively. Furthermore, the maximum and minimum numbers of zooplanktons were found in September and July which were 48.4 and 0.002 millions respectively and the average number was 8.4 ind/m^3 Table 4. Also numbers and biomass (gr/m^2) of 11 families of benthos which inhabit Gorgan Bay such as Annelida, Mollusca and Arthropoda were investigated in separated stations and distinctive months. The maximum and minimum biomass of benthos was observed in January (42.91 gr/m^2) and March (0.6428 gr/m^2). The biomass was considerable in April, February, August and December (Tables: 4, 5 and 6).
    Keywords: Ecology ; Iran ; Caspian Sea ; Gorgan Bay ; Ecological study ; Fisheries ; Ichthyoplanktons ; Phytoplankton ; Zooplankton ; Benthic ; Sampling ; Biomass ; Temperature ; Visibility ; pH ; Alkalinity ; Salinity ; Phosphate ; Cyanophyta ; Annelida ; Mollusca
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    A search for cellular and molecular cytotoxic mechanisms of Cochlodinium polykricoides in isolated rat and fish hepatocytes (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25401 | 18721 | 2018-09-18 06:00:27 | 25401 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: In this research, we investigated and compared the cytotoxic mechanisms of aqueous extract of C.polykricoides responsible for a severe and widespread HAB in the Persian Gulf and Gulf of Oman (2008-2009) in both isolated rat and trout liver hepatocytes. In addition, the role of oxidative stress and mitochondria in the induction of apoptosis were also investigated. Isolated hepatocytes were obtained by collagenase perfusion of the rat liver. To determine the hepatocyte “ROS” generation, dichlorofluorescin diacetate was used as the reagent. The uptake of the cationic fluorescent dye, rhodamine 123, has been used for the determination of hepatocytes mitochondrial membrane potential. Redistribution of lysosomotropic probe, acridine orange from lysosomes into cytosol was used for determination of lysosomal membrane damage. GSH and GSSG were determined using spectrofluorometric method. Caspase-3 activity and apoptosis phenotype were also determined using ‘‘Sigma’s caspase-3 assay kit and Sigma–Aldrich apoptosis detection kit, respectively. Incubation of algal extract with isolated rat hepatocytes caused hepatocyte membrane lysis, reactive oxygen species formation (ROS), glutathione depletion, collapse of mitochondrial membrane potential, ATP depletion and increase in ADP/ATP ratio, cytochrome c release in to the hepatocyte cytosol, activation of caspases cascade and appearance of apoptosis phenotype. antioxidants (α-tocopherol succinate and BHT), hydroxyl radical scavenger (mannitol and DMSO), Mitochondrial permeability transition (MPT) pore sealing agents (cyclosporine A, carnitine and trifluoperazine), NADPH P450 reductase inhibitor (Diphenyliodonium chloride), CYP2E1 inhibitors (Phenylimidazole and 4-Methylpyrazole) and ATP generators (L-glutamine, Fructose and Xylitol) inhibited the activation of caspase-3 and cell death. Our data showed, that algal extract activate apoptosis signaling via oxidative stress and mitochondrial pathway. ROS formation could directly be involved in mitochondrial MPT pore opening and activation of caspases cascade leading to toxic effect of C.polykricoides extract on both rat and trout hepatocytes. These findings contribute to a better understanding of C.polykricoides-toxic effects on mammalian and aquatic liver cells. Our findings revealed that trout hepatocytes are much more sensitive ( more than two hundred folds) to toxic effects of C.polykricoides extract than rat hepatocytes. On the other hand the algal extract induced lysosomal membrane damage only in trout but not rat hepatocytes.
    Keywords: Biology ; Iran ; Persian Gulf ; Oman Sea ; C.polykricoides ; Cytotoxic ; Rat and trout ; Cellular ; Molecular ; Cochlodinium Polykricoides ; Generation ; GSH ; Apoptosis phenotype ; L-glutamine
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    The study on fishing and resource management of kilka fisheries within southern Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25405 | 18721 | 2018-09-20 12:38:52 | 25405 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The project of the Study on fishing and resource management of fisheries within Southern Caspian Sea was conducted on the base of an agreement made between fisheries organization (Shilat) and the Iranian fishery research organization (IFRO) signed in 2010. The study has several phases. The final report of the fishery on fishing and resource management of fisheries within Southern Caspian Sea (Gilan, Mazandaran & Golestan provinces) is consisted of several sections such as: 1- The Survey status of biology and catch of Kilka fishes , Bony fishes and Estrogen fishes and also the status of aquatic resource conservation in the Southern part of Caspian Sea 2- Fishing analysis in the Southern part of Caspian Sea 3- Fisheries development Strategies, policies and plans in the Southern part of Caspian Sea In this survey and library-based study, internal and external environmental factors of shilat activities are considered. Using SWOT analysis method, current situation of fisheries is investigated. In this regard, all fisheries activities were divided into four groups, then, the strengths, weaknesses, opportunities and treats of internal and external environment will be analyzed. A considerable number of tables and matrixes were prepared and policies and strategies as seen of final report were carried out.
    Keywords: Fisheries ; Iran ; Caspian Sea ; Gilan province ; Mazandaran Province ; Golestan Province ; Resource management ; IFRO ; Shilat ; Fishing ; Kilka ; Fisheries ; Survey ; Biology ; Bony fishes ; Estrogen ; Environmental factors
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    Feasibility study on semi intensive polyculture of chainess and Indian carps in I.R.IRAN (Khozestan province) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25424 | 18721 | 2018-09-23 16:21:43 | 25424 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: In order to diversify the production of carp (Cyprinid) in Iran, in 2010 September, 15,000 pieces of fry from three species of indian major carp were arrived to Iran. After subtraction of the dead fishes, finally 10,500 fry were introduced to 700 m2 ponds with a density 3,500 pieces, which includes 46. 7 % Catla, (Catla catla), 35 % Rohu (Labeo rohita) and 18. 3% Mrigal Mrigal (Cirrhinus mrigala). Primary average weight and length of these fishes were 0.3±0.0 g and 23.6±0.75 mm for Rohu, 0.1±0.0 g and 33.26±0.35 for catla and 0.16±0.01 g and 34.8±0.96 mm in Mrigal respectively. Fish were reared in the Khuzestan province's climate with at least 7°C and a maximum temperature of 35°C. The culture period was 6 month. Once every 45 days by using signebeach net samples were taken from ponds and biometry tests were done. The maximum net production was observed at 50% situated treatment, with average weight 723.3 kg per 1700 m^2 ponds (4254.9 kg ha). There is no significant differences between experimental treatments with each other and with control (P 〈0. 05). Mean final weight of Rohu in 3 experimental treatments were 1136, 1129, 1054 g and in Catla and Mrigal were 726,902, 839 g and 773, 788, 894. There was not significant diference between them (P〈0.05). Survival rate in Rohu, Catla and Mrigal was 92%, 97% and 86% respectively. The maximum specific growth rate (SGR) of fish related to Catla (1.92) and the minimum SGR of fish was observed in Mrigal (1.45). In accordance with the results of this study, the Indian carp fishes can able to adapt, grow and reach to market weight in the Khuzestan province's weather conditions.
    Keywords: Aquaculture ; Iran ; Khuzestan province ; Indian carp ; SGR ; Labeo Rohita ; Cirhinus merigala ; Catla catla ; Chainess crap ; Cyprinid ; Species ; Temperature ; Survival rate
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    Effect of different heating treatments and kind of filling media on the amounts of some mineral elements (iron, zinc, copper, calcium and sodium) of silver carp (Hypopthalmichthys molitrix) during canning (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25429 | 18721 | 2018-09-23 16:29:13 | 25429 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: In this study, first, the influence of three precooking methods (steaming, oven-baking and microwave-cooking) on the contents of mineral elements-copper, zinc, iron, calcium and sodium-of silver carp (Hypophthalmichthys molitrix) was evaluated. Determination results of evaluated elements in raw fish and cooked samples were in range 3.05 to 4.19 for copper, 71.45 to 82.85 for zinc, 32.18 to 40.70 for iron, 425.6 to 529.46 and 315.5 to 534.76 for calcium. Results showed different precooking treatments had no significant effects on the amounts of mineral elements such as copper, zinc, calcium and sodium. The Iron content in the samples subjected to microwave cooking increased. With doing multivariate analysis, on comparing the raw and precooked fillets, steam cooking found to be the best precooking method on retain mineral elements. After choosing the best precooking method, for evaluating the influence of different filling media including sunflower oil, soybean oil, olive oil and brine, on the quality of canned silver carp, amounts of mineral elements(copper, zinc, iron, calcium and sodium) and microbial indices(total counts, thermopile count and clostridium) and sensory indices (color, smell, taste and texture) was surveyed. Assessments of microbial indices showed no microbial activity in canned products. After canning amounts of sodium increased in all the treatments. Except for iron, using soybean oil had no significant effect on the contents of other elements. The highest levels of iron and copper were observed in olive oil canned samples sterilized at 130°C. Sensory evaluating showed, kind of filling media had no significant effects on sensory indices such as taste, smell and color of canned samples. The texture of soybean oil canned samples and the quality defects of olive oil and brine canned samples had better condition than other treatments. In the last step, canned silver carp were proceed under three different temperatures (115°C, 120°C and 130°C) with equal lethality value (Fo=7min), then sensory indices and amount of mineral elements were compared. Results showed, the contents of iron, copper, sodium and calcium were changed in soybean oil canned sample. The amounts of copper and sodium in sunflower oil canned sample subjected to different heating regimes showed significant variation. The highest amount of copper was observed in the canned samples subjected to 130°C heating regime. Results showed contents of iron and copper of olive oil canned sample subjected to 120°C and 130°C heating regimes were higher than sample subjected to 115°C heating regime, while the zinc and calcium contents had no variation. In brine canned samples the highest amounts of copper and iron was obtained after sterilization in 120°C. Results of sensory evaluation showed different heating regimes had no significant effects on the indices of taste, smell, and color of products. Doing 130°C heating regime in brine canned samples led to obtain the better tenacity of texture. While this heating regime caused to increase the quality defects of soybean oil canned samples as a result of existence of hard parts of bone.
    Keywords: Chemistry ; Fisheries ; Iran ; Precooking ; Filling media ; Heating regime ; Sterilization ; Silver carp ; Mineral elements ; Hypopthalmichthys molitrix ; Samples ; Sunflower oil ; Soybean oil ; Silver Carp
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    A study on physico-chemicals parameters, environmental pollutants, bacterial and macrobentos distribution at the surface waters, undergrand waters and wastewater of Mangol Dam basin (Mazandaran province) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25452 | 18721 | 2018-09-26 09:35:53 | 25452 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: At the present study, the water quality parameters monthly, environmental pollutants (pesticides, heavy metals and surfactants) seasonally, macrobenthos and bacteria (monthly) were done 2009 t0 2010 that located in Mangol Dam with longitude and latitude 52° 22' 49" N and 43° 15' 43" E, respectively. All water samples were collected from under-groundwater, surface water and wastewater in 12 stations and the results are as follow: The mean concentrations of DO, BOD_5, COD, HCO^3-, CO_3^2-, TA, Ca^2+, Mg^2+, TH, TSS, TDS, Cl^-, PO4^3-, NH^4+, NO^2- and NO^3- were determined about 11.86±0.49, 5.95±0.11, 4.96±0.59, 28.1±6.1, 14.34±1.64, 36.30±2.33, 64.66±2.34, 28.84±1.52, 271.62±8.41, 0.37±0.03, 0.45±0.02, 28.02±1.96, 0.06±0.01, 0.010±0.001, 0.01±0.001 and 0.96±0.03 mg/l, respectively and also temperature, pH and EC were 12.7±0.61°C, 8.00±0.02 unit, 0.57±0.02 ms/cm in all samples. The surface water quality of Haraz River and its tributaries were suitable for usual applications such as aquatic matters, but based on BOD_5 need to more attention for human usages. The water quality index (WQI) of surface water was classified in group II at all stations that shows serious water quality changes due to domestic and agriculture sewage. The groundwater was affected by different matters that caused change of water quality parameters. The wastewater was classified from good to moderate based on BOD5. The quality of wastewater was declined at down-stream with decreasing DO and pH and increasing BOD5 pollutants. The mean concentrations of aldrin and endrin were about 0.66 ± 0.59 and 0.71 ± 1.07 µg/l, respectively. The maximum seasonal concentration of organochlorin pesticides (OCPs) were detected in fall, winter and spring for endosulfan sulfate, γ-BHC, heptachlor, endrin aldehyde compounds about 2.85, 0.34, 0.14 and 0.14µg/l, respectively. The maximum seasonal percentage range of OCPs in fall, winter and spring for endosulfan sulfate, DDD and β-BHC were 42, 25 and 25 %, respectively. The maximum seasonal concentrations during fall, winter and spring were detected in Sorkhroud and Polechelave stations for only Fe about 1.48, 3.3 and 8.22 µg/l, respectively. Also for Hg in spring was determined about 17.2 mg/l. The mean concentration of Zn, Fe and Hg in water sampled were 0.23 ± 1.01 and 1.21 ± 2.00 µg/l, respectively and 4.65± 6.38 mg/l. The maximum concentration of linear alkyl benzene sulfonate (LAS) in summer was 971µg/l. The mean concentration of surfactant during fall, winter, spring and summer was 418.62 ± 52.16 µg/l. The maximum seasonal percentage ranges of surfactant during fall, winter, spring and summer in all stations of sampling were 76, 76, 100 and 100 %, respectively. Tottaly 15 families of macrobenthos were identified in Haraz River. The maximum mean of abundance and biomass in Sorkhroud and Nour-roud stations were 1177±256 ind/m^2 and 3609±935mg/m^2, respectively. During Seasons, the maximum mean abundance and biomass were detected in fall and winter with 1185±444 ind/m^2 and 3891±849 mg/m^2, respectively. Water quality were classified by Hilsenhoff Biotic Index such as Sorkhrood station as "Very Poor", Isar town station as "Fairly Poor" and Karesang and Nor-roud stations as "good" qualification. The maximum of total coli forms and fecal coli forms in surface water were observed in Sorkhrood about 4.9 and 2.3CFU/100ml, respectively. The maximum of total coli forms and fecal coli forms in groundwater were observed in Halomsar and Absak stations about 4 and 1.7CFU/100ml, respectively. Maximum of total coli forms and fecal coli forms in wastewater were observed in Nor-roud station about 3.1 and 1.6CFU/100ml, respectively. The Ovary of nematode in wastewater were observed in Nor-roud and Isar Town stations about 30 through 124, respectively.
    Keywords: Ecology ; Iran ; Haraz River ; Mazandaran province ; Mangol Dam basin ; Physico-chemical characteristics ; Surface water ; Ground Water ; Wastewater ; Pesticides ; Heavy Metals ; Surfactants ; Macrobenthos ; Coliforms ; Nematodes ; Environmental pollutants ; Bacterial ; Temperature ; pH ; Biomass
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    Survey of diversity, distribution, abundance and biomass of macrobenthic fauna in the southern Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25496 | 18721 | 2018-10-05 07:39:50 | 25496 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The Sampling of macrobenthic communities in southeastern Caspian Sea, Iranian coast, were investigated seasonally from April to March 2012. Sampling was carried out at 8 transect as named Astara, Badar Anzali, Sefied rood, Tonekaboon, Nooshahr, Babolsar, Amirabad, Gomishan.In each transect were choice 8 stations at 5m, 10m, 20m, 50m, 100m depths respectively. Samples were taken in triplet with a 0.1m^² van Veen grab.Totally 28 macrofaunal species belonging to 10 families were identified as gamaridae (9species), corophidae (3species),psudocumidae(8 species), amphartidae (2species), balanidae, chironomidae, cardidae scorbicolaridae, nereidae, sponoidae, each one species. Polychaetes were dominated, representing 72.5% of the whole fauna while while (equal 62.4% of total abundance and 5/1%biomass) were belong to Streblospio spp. from Spionidae family. Cerastoderma lamarcki belong to bivavia consist of 2/3% total macobethos abundance and 73% biomass. Total abundance of macrobenthos of middle area were higher than east and west area in southern Caspian Sea. Average benthic population density (no/m^2) with (SE) was 4581(444 ). The benthic biomass (mg/m^2) with an overall mean of 12. 125(02). Spatial and temporal variations of benthos. In the present study showed, maximum abundance Macrobenthos 17855(5543) no/m^2) were recorded in 20 meter depths at Nooshahr trancect , while minimum 841(109 )in 10 meter depths at Amirabad trancect p〈0.05. Maximum biomass 135.10(126) g/m^2 were obtained at 10m depths in Astara minimum 0.51(0.4) ) g/m^2 at 5m depths in Nooshahr transect . maximum were obtained in winter and minimum in summer Number of species varied 3 to 18 , Generally, minimum species diversity were obtained at 20 m depth in Tonekabon transects and the maximum value was observed at 100 m depth of sefeidrood variations physicochemical factors were between salinity 12.5to12.9ppt ,temperature 8-19˚c and PH 8.1 dissolve oxygen 69-101mg/li. Total secondary production calculated, according ,104/996g AFDW/m^2/yr.Maximum were recorded in 10meter depth (30/381AFDW/m^2/yr) and minimum at 50 meter depth(11/007g AFDW/m^2/yr) respectively. Average total organic matter were 3/18 percent in sample periods. Percent of total organic matter (TOM) were low in 5 and 10 m depths and increased toward offshore depths. TOM percent was 1/67(0.11 ) at 5 m depth and increased to 4/38(0.13 )in 100 m depth. Average total silt &clay were 68/63(2.97) percent in sample periods. Positive significant relation was between silt &clay and total organic matter. Macrobenthos community reduce in high silt clay and high sandy contents. Due to increase of streptolosbio.spp, monitoring program for understanding effect of this organism on ecosystem and on fish feeding is necessary.
    Keywords: Ecology ; Iran ; Caspian Sea ; Astara ; Badar Anzali ; Tonekabon ; Macrobenthos ; Diversity ; Distribution ; Abundance ; Biomass ; Secondary production ; Species ; Gamaridae ; Corophidae ; Psudocumidae ; Amphartidae ; Balanidae ; Chironomidae ; Cardidae ; Scorbicolaridae ; Nereidae ; Sponoidae ; Salinity ; Temperature ; Streptolosbio.spp
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    The creation of genetic basic population of rainbow trout (Onchorhynchus mykiss) based on study of genetic variation in brood stocks using microsatellite (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25509 | 18721 | 2018-10-05 08:39:09 | 25509 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: In order to perform the project, 446 samples of rainbow trout (Onchorhynchus mykiss) from 24 different regions in Iran were collected. About 2-3 g of caudal fin samples was collected from each specimen and preserved in absolute ethyl alcohol and then transferred to the genetic laboratory. Genomic DNA was extracted using the phenol-chloroform method and then DNA content and quality was determined using spectrophotometry and agarose gel electrophoresis, respectively. Polymerase Chain Reaction (PCR) of genomic DNA fin samples was carried out using 10 pairs of microsatellite primers. All PCR products were electrophoresed on 6% polyacrylamide gel and stained with silver nitrate. Following the scoring of alleles, all parameters including allelic frequency, effective number of allele, observed and expected heterozygosity, shanon index, measurement of similarity and genetic distance and Hardy-Weinberg equilibrium, Fst , Rst and gene flow were calculated using AMOVA analysis in the GenAlex and Popgene programs. The results showed that 8 pairs of microsatellite primers were polymorphic. In total, 50 alleles were determined with the range size of 64-280 bp. The locus omyf had maximum number of allele (26) and loci OTSG 474 and Strurruta58 had minimum number of allele (5). The observed heterozygosity was between 0.86 and 0.964. Hardy-Weinberg departure was observed for all loci from farms 18, 15, 4, E20 and 21 and were disequilibrium (P〈0.05). The farms 14, 8, 7 and 6 were equilibrium at 3 loci, but showed disequilibrium in other loci. The other farms were equilibrium at 1 or 2 loci and disequilibrium at 8 or 9 loci. The FST results showed that maximum FST (0.24) were between farms 1 and 11in which had minimum of gene flow (3.7). Minimum FST (0.04) were between farms 8 and 9 in which had maximum of gene flow (346). Based on the results of AMOVA analysis, significant differences were detected between all farms (P〈0.01). Furthermore, based on Nei 's standard (1972) maximum genetic distance (0.89) were observed between farms 2 and 11 and maximum genetic similarity (0.15) were detected between farms 3 and 4. This result suggests that the unique genetic variation of rainbow trout in hatchery farms of Iran represents a highly valuable genetic resource and provide useful information for creating a based population in the future breeding programs.
    Keywords: Biology ; Iran ; Genetic ; Population ; Rainbow trout ; Onchorhynchus mykiss ; Brood stocks ; Microsatellite ; Samples ; Specimens ; DNA ; PCR ; ANOVA
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    Biological aspects of Sepia pharaonis in the Bahrekan waters (NW Persian Gulf) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25182 | 18721 | 2018-09-03 17:06:03 | 25182 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The biological aspects of Sepia pharaonis was studied during years 2006-07. The studied area restricted to the Bahrekan in Khouzestan province covering the depths of 2 up to 25m. The sampling methods were gillnet and bottom trawl. A total of 310 specimens collected, of which there wasn’t found any cuttlefish in the study area from July to October (5 months). The collected samples were transferred to the laboratory ashore for further biological measurements consist of: Mantle length, Body weight, sex determination. Gonado-Somatic Index, and determination of Spermatophoric Index, Spawning season, Food preference, Maturity stages and chemical analysis for food value determination. The results showed that the overall sex ratio is about M:F= 2:1 with percentage of 67.41% for males and 32.50% for females. Males are significantly bigger than females with average mantle length (ML) of 233.3 and 269.3 mm for female and male, respectively; with body weight of 1102.3 and 1450.6 g. The mantle length body weight relationship was found W=0.001 ML 2.540 (R2= 0.92) Female as: W= 0.0015 ML 4797 (R2= 0.93) male From point of feeding, the food preferences results indicated that fish is considered as main food, crabs as minor food and other marine organisms such as bivalvia and gastropods as random food. The highest vacuity Index (CV) and empty stomachs was determined for March-April and the lowest value was is December. Also, the maximum GSI was estimated for March-April months in which showing coherrances with the lowest food preference. The maximum spermatophoricfilaments were 856 and 45 for male pharaoh cuttlefish with mantle length of 300 and 185 mm, and on the other hand this values for fecundity were estimated 1589 and 53 for female specimens with 254 and 198 mm mantle length. The spawning season occurs in April- March in which accompany with migration of pharaoh cuttlefish towards shallow waters. The fishing season would be in this period in w.
    Keywords: Biology ; Iran ; Khouzestan province ; Bahrekan ; Sepia pharaonis ; Gillnet ; Sampling ; Specimens ; Weight ; Sex ; Gonado-somatic index ; Spawning ; Maturity ; Female ; Male ; Bivalvia ; Gastropods
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    Spatiotemporal and estimation of washed out seaweeds biomass in Sistan and Baluchistan coasts (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25525 | 18721 | 2018-10-05 08:33:22 | 25525 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: In order to studing and determination of Seaweed biomass in the Oman Sea coast (Sistan & Baluchestan Province), according to obtained reasults and experiments and observations on seaweed studies in 15 years ego, Beris, Chabahar, Pozm and Tang were high density zones and Jood and Lipar were low density zones in west and east of chabahar respectively that were selected for study stations. Total length of sistan and balochistan coasts from Gwatr area with geografical position 25˚ 10′ N & 61˚ 30′ E to Mydani with geografical position 25˚ 24′ N & 59˚ 5′ E were 354.3 Km. Among this length, 54.6 Km were rocky coast and 299.7 Km were sandy coast. Among this, 18.2 Km were high density zone and 281.5 Km were low density zone. Washout Seaweeds area in intertidial zones determind by measuring and recording of geographic positions by meter and GPS, then were obtained 2 transects determind with equal distance in high density area and 1 transects in low density for monthly sampling. The length of these transects were 100 meter and cross of Each transects were seaweed washed out average. The transects divided to 10 bluck and sampling were done randomly monthly from 5 bluck or 50 persent. The samples were transfered to the lab and the weight of each species was obtained after being cleaned, and separating. The biomass of each species per area unit and in the total area was obtained after determing the average weight of species and also determind geografical position by GPS. In relation to this project were done water sampling for recording of fisical chemistry factors. Total data for analysis recorded in this computer. During this research 9 species were collected. Among these seaweeds, 2 species green algea (composed of 2 families and 2 orders), 6 species brown algae (composed of 3 families and 3 orders) and 1 species red algae were identified. Wet biomass of Sargassum in the total area of Sistan & Baluchestan coasts were 445.9 ton in 2012. Among this biomas, 269.1 ton (%60.35) high density area (Beris 112 ton or %25.11, Pozm 83.7 ton or %18.76, Chabahar 59 ton or %13.22 and Tang 14.5 ton or %3.26) and low density zone 176.8 ton (%39.65). Monthly average were estimated about 74.3 ton (high density zone 44.9 and low density zone 29.5 ton). The maximum biomass was obtained 270.4 ton (high density zone 155.5 and low density zone 114.9 ton) in Azar and minimum biomass was obtained 3.5 ton in Esfand. At last, were suggested, This project were done in persian gulf coasts nessesery.
    Keywords: Biology ; Ecology ; Iran ; Oman Sea ; Sistan and Baluchistan province ; Beris ; Chabahar ; Gwatr ; Seaweed ; Washout ; Biomass ; Density ; Sampling ; Algae ; Green algae ; Brown Algae ; Red algae
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 72
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    Investigation of stock condition of Lengeh pearl oyster Pinctada radiata in western Hormozgan province habitats with emphasis on ecological agents (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25535 | 18721 | 2018-10-05 16:03:27 | 25535 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Pearling in Northern Persian Gulf has barred since 2006. Population dynamic and investigation of stock condition of pearl oysters has not done in thease area in thease years heretofore. For identification of habitats of Pinctada radiata, mohar oyster, primary sea tours in coastal waters of 11 regions in Lavan Island, 11 regions in hendorabi Island and 6 regions in Nakhiloo and Michaeil Ports, for early survey were done. 2 habitats in Lavan waters and 2 habitats in Hendorabi waters were identified and for investigation of stock condition, were sampled from autumn 2011 to summer 2012, seasonality. Belt transects andrandom quadrates method was used in this sampling. Physicochemical parameters in depth range of thease habitats was evaluated by CTD in first sea tour of stock sampling. Lavan 1 habitat, had maximum area, maximum total stock, maximum CPUA, maximum CPUE and maximum biomass, among other habitats, equal to 60ha, 357000N, 0.6N/m^2, 119N/hr and 4620kg respectively. Biometric data in Hendorabi2 habitat was rather than other habitats, significantly (P〈0.01). Infinity length, L_∞, was calculated in Lavan1, Lavan2, Hendorabi1 and Hendorabi2 habitats, equal to 89.16, 87.68, 86.11 and 86.42 mm respectively. Growth constant, K, was estimated in Lavan1, Lavan2, Hendorabi1 and Hendorabi2 habitats, equal to 0.43, 0.44, 0.47 and 0.56 year^-1, respectively. Natural mortality constant, M, was calculated in Lavan1, Lavan2, Hendorabi1 and Hendorabi2 habitats, equal to 0.73, 0.75, 0.78 and 0.88 year^-1, respectively. Results of this project showed, area of investigated habitats, oysters stock of them and CPUA, have diminished in thease years, and interdict of pearl oysters catch was not able to revival of stocks. Majority of physicochemical parameters in depth ranges in surveyed habitas, had significant difference (P〈0.01). Water turbidity in Hendorabi2 habitat was rather than other habitat, significantly (P〈0.01), as subsidiary of high sedimentation in this habitat and main reason of high natural mortality constant, M, low settlement rate and absence of lower scale of oysters length in Hendorabi2 habitat.
    Keywords: Biology ; Ecology ; Iran ; Persian Gulf ; Lavan Island ; Hendorabi ; Mohar pearl oyster ; Pinctada radiata ; Stock assessment ; Lavan Island Hendorabi Island ; Sampling ; CPUA ; Mortality ; Sedimentation
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 115
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  • 99
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    Identification of native fishes of west Azarbaijan province phase 2 (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25539 | 18721 | 2018-10-05 16:22:36 | 25539 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The aim of this research was to identify the species of freshwater fishes in the water rssoureces located at Urmieh Lake and Zagros catchment area of West Azarbaiigan Province. Sampling was done seasonally with the determination and selection of sampling station on main and secondary branches of different rivers including; Zarineh rud, Simineh rud, Mahabad chay, Shahr chay, Nazlu chay, Baranduz chay, Godar and Zab river. Fish samples catched with different tools include Electroshoker, Cast net and buy from local fisherman, fixed in 10 % formalin and transferred to the laboratory for biological and identify hnvestigation. 42 fish species belonging to 7 family comprising of Syprinidae, Balitoridae, Cobitidae, Siluridae, Poeciliidae, Salmonidae and Mastacembelidae have been identified. Among them Syprinidae family with 32 species had a highest diversity.
    Keywords: Biology ; Fisheries ; Iran ; West Azarbaiigan Province ; Identification ; Fish ; Native fishes ; Freshwater ; Syprinidae ; Balitoridae ; Cobitidae ; Siluridae ; Poeciliidae ; Salmonidae ; Mastacembelidae ; Species
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 99
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  • 100
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    Biological activity (antibacterial, antifungal, antiviral and cytotoxic) of extract from Dysidea spp. (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25555 | 18721 | 2018-10-05 16:22:32 | 25555 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Sponges are the most primitive of the multicellular, These organisms don’t have any mechanical defense system, so their early appearance in evolution has given them alot of time for the development of advanced secondary metabolites as chemical defense system. Sponges have the potential to provide drugs from chemical components against diseases. In this investigation the sponge samples, which it is Dysidea spp. , were collected at depth of 15- 20 meter, from locations on the coastline of Island Hengam in Persian Gulf of Iran. For identifying natural components, methanolic and diethyletter were used as extraction solvents, after removal of the solvents, the GC/MS spectra of the fraction were obtained. Then in vitro cytotoxic, antimicrobial, antifungal and antiviral activities were identified. In vitro cytotoxity screening, by XTT assay, against KB/ C152 and HUT-78/ C185 cell line, was conducted in this study in 1 - 500 µg/ml . IC50 for diethyletter and methanolic extract was 200 µg/ml in HUT-78 , IC50 for diethyletter extract was 325µg/ml and methanolic extract 325µg/ml in KB. In vitro antimicrobial activity by Broth Dilution Methods against clinical gram-positives and gram negatives (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus و subtilis Bacillus). The results conducted that the MIC values of methanol and diethyletter extract for Escherichia coli 20mg/ml, Bacillus subtilis 10mg/ml and 2mg/ml for Staphylococcus aureus. The MBC values of the diethyletter extracts for Bacillus subtilis 30 mg/ml) and S. aureus aureus 10mg/ml. In vitro antifungal activity by Broth Dilution Methods against clinical pathogens; Candida albicans and Aspergillus fumigatus. The results conducted that the aqueous extracts didn’t have any antifungal activities on pathogens, minimum inhibitor concentrations (MIC) of the diethyletter extract on C. albicans 0/75mg/ml, MFC 5 mg/ml and methanolic extract 0.5mg/ml and MFC 5 mg/ml on A. fumigatus In vitro antiviral activities by XTT assay against MT-2 cell line. The results conducted that IC50 for diethyletter extract 500µg/ml and methanolic extract 475 µg/ml.
    Keywords: Biology ; Health ; Iran ; Persian Gulf ; Hengam Island ; Sponge ; Cytotoxic ; Antimicrobial ; Antifungal ; Antiviral ; Methanolic extract ; Diethyletter extract ; Samples ; Escherichia coli ; Bacillus
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 118
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