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  • 1
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    Non-surgical removal of some stones from a red tailed catfish (Phractocephalus hemioliopterus) stomach as gastric foreign bodies (2018)
    Details
    Publication Date: 2021-05-19
    Description: A red-tailed catfish (Phractocephalus hemioliopterus) referred to Aquatic Animal Health Department, Faculty of Veterinary Medicine, University of Tehran for showing lethargy, bottom sitting, extension of abdomen and severe abdominal injuries. Its abdomen was swollen and the bulk of the foreign bodies were easily palpated. The heaviness of foreign bodies had made swimming and buoyancy difficult for fish. Several radiographic images were taken after anaesthetizing the catfish with 10% carvacrol, 2 drops in 1 liter of water. Radiographs revealed some stones with their size, number and location. Stones were removed non-surgically through the large oral cavity using suitable forceps. Totally 16 stones and 2 shells were removed from the stomach of the fish. The catfish was then returned to the water where normal swimming and buoyancy was restored.
    Description: Published
    Keywords: Catfish ; Stones ; Foreign body ; Radiography
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.35-42
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  • 2
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    The study of skin lesions in Beluga (Huso huso) fed with different dietary levels of Aflatoxin B1 (2018)
    Details
    Publication Date: 2021-05-19
    Description: In this study, 180 Beluga (Huso huso) weighing 120110g each with a stocking density of 12 fish per tank were adapted with synthetic diet and then fed with different levels of AFBI (0, 25, 50,75 & 100pplpikg of diets) under controlled conditions (T=1 8±2°C, DO=7.3ppm). The fish were fed 3% of their body weight four times a day for three months. Prevalence of skin lesions evaluated through clinical observations monthly. With regard to toxin concentration and time of exposure to AFBi in experimental fish, different degree of skin lesions (simple hemorrhage to progressive wounds) were observed in different parts of body especially in vent, caudal peduncle, fins, and head. "Yellow sores" on head and trunk regions were considerable and led to deterioration of appearance. Prevalence of skin lesion in different treatments was 8•m53.3%. After feeding with toxic diets was stopped, 16.w24% healing was obsenTed in the fish.
    Description: Published
    Keywords: Huso huso ; AFB ; Oral Administration ; Skin lesions ; Beluga ; Lesion Healing ; Feeding
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.43-52
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  • 3
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    Introducing genetic markers to identify and distinguish five species of Cyprinidae in the Caspian Sea using PCR-RFLP (2018)
    Details
    Publication Date: 2021-05-19
    Description: Cyprinids are the main and most significant bony fishes found in the Caspian Sea. In this study, 15 specimens of Rutilus fish kutum (Kamensky, 1901), 10 specimens of Barbus capito (Gueldenstaedtii, 1772), 10 specimens of Bream Abramis brama (Linnaeus, 1785), 10 specimens of Redlip, Aspius aspius, and 15 specimens of Kora volba, Rutilus rutilus caspius, were collected from commercial catch stations. DNA from all specimens was extracted using the phenol-chloroform method in 500[1.1 tubes and amplified using PCR method with a pair of primers with cytochrom b gene sequence of Kora volba. For RFLP analysis, PCR products of cytochorome gene b (1117 bp) from each species were digested with five restriction enzymes under suitable conditions of incubation. DNA bands were visualized by gel electrophoresis (polyacrylamide) and staining with silver nitrate. Five enzyme Rsa I, Hinf I. Hha I , Hine II, and Mbo I showed polymorphism. Genotypes obtained from digestion of enzymes Rsa I, Hinf I, Hha I showed haplotypes AAAAA for Barbus capito, BBBB for R. rutilus caspius, CCCC for Bream Abramis brama, DDDD for Aspius aspius and EEEE for R. frisii kutum. Each of these haplotypes serves as a genetic marker for the species and is of significant importance in distinguishing them.
    Description: Published
    Keywords: Cyprinidae ; PCR-RFLP ; Barbus capito ; Bream Abramis brama ; Aspius aspius ; Rutilus rutilus caspius ; Aspius aspius ; Polyacrylamide ; Molecular Marke
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.49-58
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  • 4
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    Introducing genetic markers to identify and distinguish five species of Cyprinidae in the Caspian Sea using PCR-RFLP (2018)
    Details
    Publication Date: 2021-05-19
    Description: Cyprinids are the main and most significant bony fishes found in the Caspian Sea. In this study, 15 specimens of Rutilus frisii kutum (Kamensky, 1901), 10 specimens of Barbus capito (Gueldenstaedtii, 1772), 10 specimens of Bream Abramis brama (Linnaeus, 1785), 10 specimens of Redlip, Aspius aspius, and 15 specimens of Kora volba, Rutilus rutilus caspius, were collected from commercial catch stations. DNA from all specimens was extracted using the phenol-chloroform method in 500gl tubes and amplified using PCR method with a pair of primers with cytochrome b gene sequence of Kora volba. For RFLP analysis, PCR products of cytochrome gene b (1117 bp) from each species were digested with five restriction enzymes under suitable conditions of incubation. DNA bands were visualized by gel electrophoresis (polyacrylamide) and staining with silver nitrate. Five enzyme Rsa I, Hinf I, Hhal, Hinc II, and Mbo I showed polymorphism. Genotypes obtained from digestion of enzymes Rsa I, Hinfl, flha I showed haplotypes AAAAA for Barbus capito, BBBB for R. rutilus caspius, CCCC for Bream Abramis brama, DDDD for Aspius aspius and EEEE for R. frisii kutum. Each of these haplotypes serves as a genetic marker for the species and is of significant importance in distinguishing them.
    Description: Published
    Keywords: Genetic ; Species ; Cyprinidae ; PCR-RFLP ; Molecular Marker ; Rutilus frisii kutum ; Specimens ; Barbus capito ; Bream Abramis brama ; Aspius aspius ; Rutilus rutilus ; DNA ; Enzymes
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.49-58
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  • 5
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    Recognation of fishes in rivers and basin of Qom (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This study was carried out on rivers, basins & qanat of Qom region during the 1376-1378and Fish samples were taken from 2 permanent rivers (Qomrood ,Ghara_chag)& from some seasonal ones (Tagharood, Zavarian, Vesva, Biraghan). Also local fishes was followed in 100 qanats in the region and Random fish sampling was done in permanent river extension out of province. This study aimed to recognize different fish species in the province water reservoir and estimating the fisheries potential in the province. For fish sampling nets such as mashk, salik, hook and hand tailored were used. Electroshoker was not used at all. Results of fish recognition showed that fish samples belong to 12 species & 4 family of "cyprinidae", "Balitoridae", "cyprinodontidae" and "poecilidae".The qanat fishes of the montain (kahak, Khagestan) and pastora area (Ghanavat,jafarabad) included by two families of "cyprinid", "Balitoridea".In some qanat which were connected to rivers more species of fish and anotherAquatic animal were observed & sampled.At the and map of geographical distribution of local fishes was planned.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Geographical ; Fish ; Samples ; Species ; Fisheries ; Electroshoker ; Cyprinidae ; Balitoridae ; Cyprinodontidae ; Poecilidae ; Aquatic ; Recognation
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 67pp.
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  • 6
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    Recognition of fishes in rivers and basin of Qom (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25143 | 18721 | 2018-08-26 16:50:02 | 25143 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This study was carried out on rivers, basins & qanat of Qom region during the 1376-1378and Fish samples were taken from 2 permanent rivers (Qomrood ,Ghara_chag)& from some seasonal ones (Tagharood, Zavarian, Vesva, Biraghan). Also local fishes was followed in 100 qanats in the region and Random fish sampling was done in permanent river extension out of province. This study aimed to recognize different fish species in the province water reservoir and estimating the fisheries potential in the province. For fish sampling nets such as mashk, salik, hook and hand tailored were used. Electroshoker was not used at all. Results of fish recognition showed that fish samples belong to 12 species & 4 family of "cyprinidae", "Balitoridae", "cyprinodontidae" and "poecilidae".The qanat fishes of the montain (kahak, Khagestan) and pastora area (Ghanavat,jafarabad) included by two families of "cyprinid", "Balitoridea".In some qanat which were connected to rivers more species of fish and anotherAquatic animal were observed & sampled.At the and map of geographical distribution of local fishes was planned.
    Keywords: Biology ; Iran ; Qom province ; Qomrood ; Ghara_chag ; Tagharood ; Fish ; Samples ; Species ; Fisheries ; Electroshoker ; Cyprinidae ; Balitoridae ; Cyprinodontidae ; Poecilidae ; Aquatic ; Recognation
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 67
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  • 7
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    Effects of different dietary levels of AFB1 on survival rate and growth factors of Beluga (Huso huso) (2021)
    In:  http://aquaticcommons.org/id/eprint/22361 | 18721 | 2018-03-27 22:46:13 | 22361 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-04
    Description: In the present study, the impacts of various concentrations of Aflatoxin B1 (AFB1) on Beluga, Huso huso, under controlled conditions were investigated. Belugas (120±10g) were fed on diets containing 0, 25, 50, 75, and 100 ppb AFB1/kg of diet for 3 months. Results showed various levels of AFB1 do not significantly affect the specific growth ratio (SGR) (P〈0.05) of fish in different treatments. However, weight gain and food conversion ratio (FCR) were varied significantly (P〈0.05 between control and treatments with diets contaminated with 75 and 100 ppb AFB1/kg after 90 days). The increase AFB1 level of did not affect on the percent of survival rate (SR) and no mortality was observed in treatments (SR=100%) suggesting that various AFB1 levels under experimental conditions of the present study affect some growth factors, such as, weight gain and FCR but have no significant impact on SR and SGR. Histopathological studies showed that different level of AFB1 can cause broad range of change in liver tissue, including progressive fat deposition, hepatocyte degeneration and necrosis, particularly at concentration of 75 and 100 ppb AFB1/kg of diets after 60 days.
    Description: Article includes abstract in Farsi on last page.
    Keywords: Biology ; Fisheries ; Huso huso ; AFB1 ; FCR ; SGR ; SR ; Pathology ; Liver ; Beluga ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 141-150
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  • 8
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    The study of skin lesions in beluga (Huso huso) fed with different dietary levels of Aflatoxin B1 (2021)
    In:  http://aquaticcommons.org/id/eprint/23783 | 18721 | 2018-07-27 13:48:55 | 23783 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-15
    Description: In this study, 180 Beluga (Huso huso) weighing 120110g each with a stocking density of 12 fish per tank were adapted with synthetic diet and then fed with different levels of AFBI (0, 25, 50,75 & 100pplpikg of diets) under controlled conditions (T=1 8±2°C, DO=7.3ppm). The fish were fed 3% of their body weight four times a day for three months. Prevalence of skin lesions evaluated through clinical observations monthly. With regard to toxin concentration and time of exposure to AFBi in experimental fish, different degree of skin lesions (simple hemorrhage to progressive wounds) were observed in different parts of body especially in vent, caudal peduncle, fins, and head. "Yellow sores" on head and trunk regions were considerable and led to deterioration of appearance. Prevalence of skin lesion in different treatments was 8•m53.3%. After feeding with toxic diets was stopped, 16.w24% healing was obsenTed in the fish.
    Keywords: Fisheries ; Health ; Huso huso ; AFB1 ; Oral Administration ; Skin lesions ; Lesion Healing ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 43-52
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  • 9
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    Introducing genetic markers to identify and distinguish five species of Cyprinidae in the Caspian Sea using PCR-RFLP (2021)
    In:  http://aquaticcommons.org/id/eprint/24743 | 18721 | 2018-08-04 17:09:13 | 24743 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-15
    Description: Cyprinids are the main and most significant bony fishes found in the Caspian Sea. In this study, 15 specimens of Rutilus fish kutum (Kamensky, 1901), 10 specimens of Barbus capito (Gueldenstaedtii, 1772), 10 specimens of Bream Abramis brama (Linnaeus, 1785), 10 specimens of Redlip, Aspius aspius, and 15 specimens of Kora volba, Rutilus rutilus caspius, were collected from commercial catch stations. DNA from all specimens was extracted using the phenol-chloroform method in 500[1.1 tubes and amplified using PCR method with a pair of primers with cytochrom b gene sequence of Kora volba. For RFLP analysis, PCR products of cytochorome gene b (1117 bp) from each species were digested with five restriction enzymes under suitable conditions of incubation. DNA bands were visualized by gel electrophoresis (polyacrylamide) and staining with silver nitrate. Five enzyme Rsa I, Hinf I. Hha I , Hine II, and Mbo I showed polymorphism. Genotypes obtained from digestion of enzymes Rsa I, Hinf I, Hha I showed haplotypes AAAAA for Barbus capito, BBBB for R. rutilus caspius, CCCC for Bream Abramis brama, DDDD for Aspius aspius and EEEE for R. frisii kutum. Each of these haplotypes serves as a genetic marker for the species and is of significant importance in distinguishing them.
    Keywords: Biology ; Cyprinidae ; Molecular Marke ; PCR-RFLP ; Caspian Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 49-58
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  • 10
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    Bioassay of combined hydrogen peroxide and silver ion at four life stages of Indian white shrimp (Fennerop enaeus indicus) (2021)
    In:  http://aquaticcommons.org/id/eprint/23841 | 18721 | 2018-07-31 06:59:20 | 23841 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-15
    Description: Silver ion and hydrogen peroxide act synergistically as a strong disinfectant. The purpose of this study was to assess the effect of combined hydrogen peroxide 50% and silver ion 0.05% at four life stages of Indian white shrimp (Fenneropenaeus indicus) and to evaluate the feasibility of using this substance in shrimp culture. The Trimmed Spear man karber software was applied for determining EC _(50)/96h and LC sub(50)/96h with 95% confidence limit on the 6000 shrimp based on OECD static method. The experiments were conducted in Iran Shrimp Research Center, Bushehr, during 2005 -2006. The EC _(50) values of 132.5, 67.89, 55.56, 51.95 and 48 .6ppm were obtained in PL15 stage after 12, 24, 48, 72 and 96 hours, respectively. Also these were 147.57, 70.83, 60.01, 54.89, 41.19 for PL_(45) stage, and 306.43, 174.14, 113.62, 78.21, 61.96 for sub adult stage (12 plus or minus 1 grams), res pectively. In addition, the EC _(50) values of 243.25, 130.55, 75.56, 61. 18 and 51.59ppm were obtained at adult stage (20 plus or minus 2 grams), respectively. The LC sub(50) values of 239.81, 101, 74.28, 65.72 and 61.45ppm were obtained in PL _(15) stage after 12, 24, 48, 72 and 96 hours, respectively. Also thes e were 304.56, 160.12, 113.1, 93.69, 79.38 for PL _(45) stage, and 712.13 , 518.44, 265.29, 145.53, 103.76 for sub adult stage, respectively. In addition, the LC _(50) values of 827.75, 508.91, 317.3, 139.44 and 85.88ppm were obtained at adult stage, respectively. The statistical results showed that; no observed effect concentration" (NOEC) of this substance was 20ppm, and the "lowest observed effect concentration" (LOEC) was 40ppm, thus "maximum allowable concentration" (MAC) value was determined 28.8 ppm on the Indian white shrimp. Therefore this combined chemical should be used under determined MAC value with a complete precautionary as a disinfectant for Indian white shrimp.
    Keywords: Biology ; Microbe ; Disinfect ; Bioassay ; Indian white shrimp ; Fenneropenaeus indicus ; Bushehr Province ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 129-138
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