ALBERT

Description: Marked increases of CO2, H2 and He dissolved in thermal waters and changes in the dissolved carbon isotopic composition, were observed at Stromboli before the 28 December 2002 eruption and before a violent explosive paroxysm occurred on 5 April 2003. High anomalous CO2 flux values were recorded at the crater rim since a week before the eruption onset. The first anomalies in the thermal waters (dissolved CO2 amount) appeared some months before the eruption, when magma column rose at a very high level in the conduit. High peaks of dissolved H2 and He were recorded a few days before the paroxysm. Carbon isotopic composition indicates a magmatic origin of the dissolved CO2 whose increase, together with those of H2 and He, is attributed to an increasing output of deep gases likely produced by depressurization of a rising batch of a deep gas-rich magma, whose fragments have been emitted during the explosion.

Description: Italian Civil Protection

Description: Published

Description: L07620

Description: partially_open

Description: Qhar-khetlu dam reservoir is in the central part of Ijrud city of Zanjan province with a reservoir volume of 500000 and a water volume of 900000 cubic meters, covering an area of 6 hectares and containing 3500 hectares of the watershed in order to control seasonal floods, drinking water in the village, strengthening the aquifers of the area and As a water supply in the dry seasons, 120 hectares of agricultural land was planted for irrigation.Study of this water source with the aim of measuring biological and non-biological factors, determination of aquaculture production and Fish release capacity and fishing capacity for optimal use of fishery management in Zanjan province was done in 2006.The results of hydrochemical analysis of the water of Qharkhetlu dam reservoir showed that the minimum and maximum temperature of water ranged from 4.5 to 26 ° C, the pH of the water was 4.7 to 8.8, the dissolved oxygen was 7.7 to 12.2 mg, the total water hardness fluctuation was 154 194 mg / L and electrical conductivity of 272 to 390 micrometers per square centimeter, the water transparency was 25 to 380 centimeters.In this study, six phytoplankton classes with 31 genera and 3 classes of zooplankton with 14 genera were obtained. The abundance of phytoplanktons was counted from 150,000 to 206,000 per liter, and the average chlorophyll a, 1.10 micrograms, was estimated. The frequency of zooplanktons were 32 to 132 per liter, as well as the mean of 544 per square meter of macrobenthos.By comparing of the physical, chemical and biological factors and also the results of previous studies of this water source, the conditions for the introduction of warm water and cold water fishes including silver carp, big head carp, rainbow trout out and native fishes including species of Barbus and Capoeta genera are suitable. Estimation of Qhar-khetlu dam reservoir production is 80 kg / ha and its production capacity is 484 kg per year.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was conducted to determine physico-chemical characteristics of water and their spatial and temporal fluctuations in the Mazandaran coastal of Caspian Sea in 6 months, four seasons at 4 transects (Tonekabon, Nowshahr, Babolsar, Amirabad) during 2012-2013. 72 samples were collected at surface layer of water in 5, 15 and 30m depths. Then the sampls analyzed based on standard methods. Results showed that the mean of water and air temperature were 19.46±0.85 and 20.25±0.78 ◦C, respectively. Minimum and maximum of water temperature were recorded in winter (7.00) and summer time (28.10). Mean of salinity was 12.35±0.13 ppt. The maximum salinity was recorded in summer and minimum in winter in all transects. Mean of transparency (SD) in the present study was 2.63±0.18m. The mean of pH was observed 8.51±0.02 which was higher than the pervious sampling periods. The mean of DO was observed 6.00±0.07 ml/l during sampling period. Annual concentration of dissolved inorganic nitrogen (DIN= NH4 +, NO2-, NO3-) has a fairly wide variation in diferents months and transects. Percentage of nitrogen components out of DIN were varied 6-53, 0.14-26.0 and 37.0-94.0 respectively. In this study, percentage of DIN was lower than 15% and dissolved organic nitrogen (DON) was higher than 80%. Mean of annual dissolved inorganic phosphorous (DIP) and organic phosphorous were 0.58± 0.04 and 0.48± 0.02 µM, respectively. DIP and DOP percentages recorded 54 and 46 respectively. Mean annual of dissolved silicon (DSi) obtained 9.5± 0.2 µM. Based on the results, the system was in phosphorus limitation during spring and summer but it shifted to nitrogen limitation in autuman and winter. Phytoplankton development was not limited by DSi at any seasons.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was conducted to determine growth parameters, catch and fishing effort trends, stock assessment and Acceptable Biological Catch (ABC) of bony fishes in the Iranian coastal waters of Caspian Sea in the years 2010-2011 and 2011-2012. According to the result, the numbers of beach seines were 131 and 128 and their fishing efforts were observed 50184 and 42255 beach seining during 2010-11 and 2011-12, respectively. The catch per unit of effort CPUE) was calculated 182.9 and 205.6 kg/haul during two sampling periods, respectively. The total catches (including illegal fishing) were also obtained 16601.5 mt and 17034.1 mt during 2010-11 and 2011-12, respectively. The highest proportion of catch was belonged to kutum and golden grey mullet (86% and 88%, respectively) in two fishing seasons aforementioned above. Growth parameters of kutum were estimated as K=0.21/yr, L∞ = 60.0 cm, t0 = 0/yr. The Growth parameters were K=0.18/yr, L∞ = 61.1 cm, t0 = -0.14/yr for golden gray mullet and were K=0.12/yr, L∞ = 73.6 cm, t0 = 0.92/yr for common carp. Based on catch-at-age data, in the years 2010-2011 and 2011-2012, the total biomass, from the biomass-based cohort analysis were estimated 41700mt and 34400 mt for kutum and 14600 mt and 14400 mt for golden grey mullet, respectively. The reference points of F0.1 and F35% were 0.41/yr and 0.34/yr for kutum and 0.36/yr and 0.33/yr for golden grey mullet, respectively. Stock enhancement plays an important role in recovery of kutum stocks in the Iranian coastal waters of Caspian Sea. There is a significantly negative correlation between fingerlings released and condition factor (CF) and recruitment and CF. The different trends for fingerlings, recruitments and CF suggest that CF may be partly density-dependent, declining at high population sizes due to intra-specific competition. Therefore, more research should be conducted to determine the desirable level of artificial propagation. Food consumed by fish species, Rutilus frisii kutum, Rutilus rutilus caspicus, Cyprinus carpio, Liza auratus and Liza saliens were included Foraminifera, Porifera, Annelida, Mollusca, Arthropoda, filamentous algae, fish eggs and detritus.The results showed that Rutilusfrisii kutum generally feed on Balanus and Cerastorderma. The main food item for Cyprinuscarpio, Liza auratus and Liza salienswas detritus. Based on available models, the ABCs were estimated as 6600-7400 mt for kutum and 2200-2800 mt for golden grey mullet (with precautionary approach 6600 mt and 2200 mt for kutum and mullet, respectively) in 2011-12. Two species (kutum and golden grey mullet) are vulnerable to environmental factors, and these factors should be considered in the stock assessment and management of the fish. For two species, the ABC with a lower andmore accurate value based onmore information, should be selectedfor the implementation of a precautionary management approach.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: A sharp decline in natural population of Caspian brown trout lead to implementing of restocking program in Iran. Along the stock recovery program, the artificial production of this species has been attracted too much attention in recent years. The objective of the study is to assess the effects of protein and energy levels of diet on fish performance, growth efficiency of Caspian brown trout in order to develop a proper diet for production of this species. Six experimental diets were formulated by replacing casein as a pure protein source with fat as an energy source in the diets according to a 3×2 factorial design. The diet was assigned to 18 tanks with 50 fish each, with three replicated for each diet. The experiment was conducted for 8-weeks with Caspian brown trout with an initial body weight of around 7 g. Caspian brown trout demonstrated a better FCR and a larger SGR at lower protein levels (45 and 50%) in comparison to high protein level (55%; P〈0.05). Protein efficiency ratio improved by a decrease in protein content of the diet (P〈0.05), but energy content of diets does not affect any growth related parameter such as FCR, SGR and PER. There was also no interaction between protein and energy levels in the growth related parameters, suggesting the effect of protein on the growth parameters in Caspian brown trout did not depend on energy levels of diet. In conclusion diet containing 50% protein can support the maximum growth. High dietary energy level of the diet dose in cooperate in protein sparing effect.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In order to domesticate Litopenaeus vannamei as a solution to single species shrimp aquaculture of Penaeus indicus and to reduce risk of shrimp culture in emergency cases, this study was carried out. Initially the postlarvae reared from imported L. vannamei were cultured. After culture season the adult shrimps were transported to greenhouse ponds for wintering. It is then relocated to hatchery saloon. After eye stalk ablation and maturation the female brood stocks were introduced to matured male for copulation at ponds. During the study period, male and female shrimps attained full maturation at end – April by temperature over 25ºC, the copulation process was started significantly. Finally of the 12 instances of copulation, one instance resulted nauplii production. The best maturation was observed at 28 ֯C ±1 and salinity30 to 33PPT and pH of 8 to 8.3 Fresh sea worms and squid showed the highest effect on fecundity in brood stocks. Besides, the spring season was obtained to be the best period for brood stock production in Bushehr province.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Following to introduce Vannamei shrimp (Litopenaeus vannamei) into Iran aquaculture industries by fisheries organization its postlarvae were successfully reared in ponds in Bushehr and Choebdeh in Khuzestan. Due to activity of Shrimp site and importance of input and output water quality, this study was done in 11 stations including: two stations in Bahmanshir river, two station in C4 and C5 irrigation canals, six staions in active farms along C4 cannel (Yonesi, Mosavei and Salman Zadeh), and C5 cannel (Mohamadi, Khairi and Ashraf Por) and one staion in output draing were selected. Sampling were carried out from May2009 befor beginning stocking to harvest time in October 2009. Physico- chemical parameters and plankton sampled biweekly and benthic animals and heavy metals were sampled monthly. Some parameters such as pH and temperature by using portable multy parameters and others. Analyzed with standard methods. Heavy metals were measured by plarography method (797 Vamtro). Plankton and benthos samples were studied only in Bahmanshir station. According to results the maximum values for DO (11.1 ppm) PO4 (1.86 ppm) NO3 (8.4 ppm) and TSS (4992 ppm), pH (8.4) NO2 (0.18 ppm) BOD5 (9.06 ppm) were measured. In comparison to aquaculture water quality standards except for TSS in river stations others are located in allowable range. We didn’t observed any main changes in water quality from river to output drainage. The results of biotic parameters in river stations showed that diatoms were the most group of phytoplanktons. Zooplankton groups were Copepods, Rotifera and Protozoa. Tintinids and Nematoda larvae were the most abundant zooplankton. Benthic animals were included nine crustacean species and two annelid species. Isopods with 52% were the most and ant group.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Carp aquaculture is one of Maine potential for commercial and rural development , employing enhancement and earning income .This enterprise which is conducted by using fresh water and unfertile lands have been attended in the country since last seven decades. In accordance to wide area of cultivation in the country, maximum production is about 150000 tonnes in 2010. Establishment of aquaculture center by technical deputy of president, made a suitable opportunity for preparation of carp development road map. In this report, present condition and problems in front of carp development have been analyzed , and regarding to targets of long term country development, projects for research and executive sectors have been prepared.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Use of underground inland water for fisheries aims is improved all over the world. Tilapia is the first candidate for brackish water fish culture and an economic fish based on its biological characteristics. Tilapia was entered to Iran for the first time in November 2008. Bafq area at the center of Iran was licensed by Environment Organization, because of closed underground water resources of the area. Studies showed desired growth and adaptation of Nile tilapia in brackish water earth ponds and fiber glass tanks with high survival and low FCR. Limnological factors were suitable for tilapia culture. All male samples were made using certain doses of methyl testosterone in defined period. Optimum level of protein, fat and energy were determined for the best growth and food performance of cultured tilapia. Environmental program was presented to prevent environmental problems. However, studied shoed tilapia culture had not negative effects in the area. Economical performance of tilapia culture project was desirable. Tilapia was successfully introduced to some private fish farms in June 2011. Tilapia culture may improve in center of Iran after environmental surveys.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The silver pomfret, Pampus argenteus, is distributed in Indo-western pacific ocean, especially in Persian Gulf and Oman sea, and although is widely commercially exploited, the genetics structure of this species is not clear completely. In the present study, the genetic diversity and population structure of Pampus argentus in the Persian Gulf and Oman Sea were investigated using microsatellite DNA markers. Genomic DNA from 125 specimens was extracted and PCR amplification performed. Seven loci with reasonable polymorphism were amplified. The results showed that average of observed heterozygosity was ranged from 0.54 while expected heterozygosity was 0.67. After applying the Hardy-Weinberg Equilibrium (HWE) test, some loci were found to be significantly deviated from HWE in some populations in which deficiency of heterozygotes was apparent. Population differentiation observed among all populations (P 0.01). The population differentiation (Fst) value between Kuwait and bushehr was the highest and significant (0.087) and between Chabahar and Bushehr (0.021) was the lowest and significant. Genetic distance analysis showed that the largest distance (0.269) was between Khuzestan and Chabahar and the smallest distance (0.075) was between Bushehr and Chabahar. The reported results could be of interest for management and conservation programmes of this species in the Persian Gulf and Oman Sea.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Menmiopsis leidyi was entered into Caspian Sea from Black Sea and mass population constitutes in the southern Caspian Sea. In this surveys, the relationship between length and wet weight measured and length and weight followed by this equation: n= 384, R^ 2=0.87 w= 0.003 L^ 9005 . The relationship of dry and wet weight calculated, in this surveys, the ratio of dry weight to wet weight was equal to 1.615% in 12 salinity. In vitro, the clearance rate at 12 and 27 C were 52.48 and 107.33 ml/h, respectively, daily ration at above temperatures were 1.98 and 16.65 respectively. Mnemiopsis leidyi can feed Acartia in accordance to 16.5 mg/1gbw (body weight) at 27 C in one day. The average digestive time in 12 C and 27 C were 77.50 min and 36 min respectively. The digestive value in 27 C was 2.5 fold than 12 C . The result indicated that clearance rate, daily ration and digest were increased in high temperature. Stomach content of Mnemiopsis leidyi was shown that this organism utilized from Acartia and Balanus, in addition, stomach content of kilka including two species of zooplankton from the southern Caspian Sea. Stomach contents of clupeonella cultiventris contain more than 54 percent of Acartia and its nauplii. This frequency observed in cluponella engrauliformis and clupeonella grimmi. On basis of zooplankton sampling in the sea, over 90 percentage of zooplankton populations contain Acartia.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: According to achieve sustainable production in shrimp farming industry, it is necessary to provide the relief of producers through insurance. Aquaculture faces various environmental conditions such as unpredictable climate changes, epidemic diseases, harsh water factors, that may affect the production cycle, and impact on producer's life. In this research, the effects of various factors on production of cultured shrimp have been studied. The project conducted through completion of questionaries by random selected of small scale farms, and all large farms which engaged in production in that years. In order to increase the accuracy of responses, special questionnaires provided to fill in by expertise experts.All data explained the share of management and natural factors on production process.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: To accomplish the stock assessment that pre-planned for bony-fish resources investigation started by considering two pivotal act. First gathering biometric data and second using catch statistics information for stock analyses. Implementing the research programmes five itinerant groups in Anzali, Keyashahr, Noshahar, Babolsar, Torkaman have been organized during fishing season. The biometric data were supplied in computer that distincted by each fish species and subdivided into age groups. In analyses the samples, Bertalanffy formula used for calculating average length and age, also for growth coefficient. Total mortality rate calculated by using Pauly formula, and the biomass status estimated through Cohort analysis. The catch by beach seiner cooperatives recorded 8630 metric tons, but estimation including. Poacher catch raised the harvest to 15.6 thousand tons. The total catch of Kutum reduced by 1400 tons compared with previous year, but the estimation revealed the biomass of Kutum 18.5 thousand tones, the stock stowed reduction in quantity relative to past years. Harvest exceeding the limit, fishing pressure. And employing higher fishing effort, combining releasing the Kutum seedling with shorter average length in recent years, leaded the shrinkage of biomass of Kutum resources. The Golden Grey mullet catch has been estimated 3437 metric tons, the figure showed 1500 tons increment, in comparison with previous year, nearly 24% of catch was shorter than Lm50% of this species. In Guilan province higher number of sub - size golden mullet captured, this development occured less in Mazandaran and Golestan province. Higher rate of exploitation, reduction of catch in recent years, dominating small size fish in catch composition indicated the population undergoing stress in ecosystem. Thin - lipped grey mullet only comprised 9.2% of the mullet catch - Length - structure of this fish species population formed mainly by young small size fish, the biomass of latter estimated 1100 metric tons. Catch further than maximum sustainable yield, by higher exploitation rate, and gradual diminishing of harvest, represented the resources of this fish species suffering both by high fishing pressure and un - sustainable effort, which ultimately declined the fishery of this fish species. Carp catch and its resources in Anzali region become very low, it is necessary to enhance the stock by Sea-ranching programmes. The carp resources in Gorgan region enjoy better status, but catch practices do not compatible with rule and regulation in Gorgan and Gomeyshan area. The standing stock of Gorgan carp estimated nearly 6000 tons. The information concerning carp resources indicate the stock experiencing pressure and likely the catch will diminish in future. The total catal catch of carp calculated 1900 tons. In recent years the Caspian roach resources are increasing but the bulk of harvest fished illegally and by gill - nets. The total catch of the roach during 1998-1999 estimated 1700 tons. Considering the semi - artificial propagation of roach in Golestan province, if has been concluded the multiplication of this fish species also should be planned by fisheries authorities for Anzali region. It is also advisable catching of this fish species to be regulated soon and selective catch are proposed. The catch of pike - perch during the year of 1997 - 98 jumped to 95 tons, but 90% of catch formed by young fish or sub – standard length size. Almost, all the increment of catch attributed to the releasing of millions of seedlings in the Sea which the plan has been implemented by Iranian Fisheries. Increasing the numbers of released seedlings, with higher average weight Identification of most suitable site for unloading the fries could ensure better survival rate and contribute the enhancement of resources and produce good catch for this desirable fish species. The total catch of Bream hit 18 tons, but most of the catch comprised by young fish with sub - standard size. The present formation of fisheries population of bream are the result of seedling releasing! In the Sea by Iranian Fisheries, since the catch of bream are negligible despite releasing programmes of seedling in a decade which 14million individuals until now, one could conclude the programmes do not contributed effectively to stock rehabilitation. The Caspian Barbus (Barbus brachycephalus) are at the threshold 01 vanishing, at present a few numbers are caught, therefore B.capito appeared in catch composition. The catch of latter species in recent two years are increasing. Total catch during the year of 1998 - 99 estimated 34.5 tons, biomass calculated 93 tons. Caspian vimba are small size fish which beach seiner (mesh size in bosom 30 millimeters) could not capture them ideally, bulk of the catch caught in illegal manner by gill nets. The total catch estimated 154 tons which the present fishing procedures surpassed total allowable catch (136 tons). In recent years the catch of this fish species are increasing. "Shemaya' similar in size with Vimba are caught less by beach seiner, illegal catch are considerable, the total catch in 1998 - 99 estimated 86 tons. The level of harvesting of this fish species are increasing in recent years and its population size become significant relative to last years. The main part of catch concerning Aspius fish species occured in Guilan coastal area, high fishing pressure reduced the population size, including low discharge of water in rivers during spawning period, impact negatively to reproduction. Catch statistic in 1989 showed 128 tons of this fish species are caught but in 1998 - 99 it has been reduced to 6.8 tons. It has been suggested artificial spawning could prevent its fate as endangering species. Caspian salmon catch estimated 78 tons during 1998 - 99, salmon population in Caspian Sea are supported by releasing reared smolts, which in recent years nearly 500 thousands fingerlings released in suitable rivers. After banning the gil nets application in Southern Caspian Sea, the catch of salmon in beach seining are increasing. It is necessary the released smolts in rivers being protected for its safe approach to Caspian Sea. The total catch of herring fish species in 1998 - 99 were nearly 700 tons. The bulk of the catch occur In Guilan fishing area, elimination of gill - nets usage in Southern Caspian Sea contributed the increment of herrings catch in beach seining. The herring resources in Caspian Sea reduced relative to past decades. The catch during 1913 – 1916 ranged between 130 - 160 thousand tons, in Caspian Sea.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Striped grey mullet (Mugil cephalus) is a marine fish with high commercial value and widespread distribution. The grey mullet and some other mullets can be found in saltwater, brackish and fershwater habitats at tropical, subtropical and also temporal climates.This species is suitable for aquaculture worldwide due to living in wide ranges of water temperatures and salinities, acceptable growth rate, suitable food conversion ratio, marketability, suitable species for polyculture with shrimps, marine fish and even with freshwater carps. The grey mullet is farming in different countries such as Italy, Northern Africa countries ( Egypt), occupied Palestine, India, Pakistan, Japan, Hong Kong, Taiwan, Vietnam, Indonesia, coastal countries of southern pacific ocean and Hawaii. Ten thousands of fries of this species (average weight and length of 280 mg and 28.3 mm respectively) imported in March 2008 from Egypt. The wild fries colleted from surrounding natural waters of Alexandria. After finishing the quarantine steps, they stocked in four earthern ponds (each with 2500 m2) located at the Gomishan shrimp educational centre at late march in 2008. At the first year of culture the densities were 5000 and 10000 individuals per hectar and each treatment with two replicates. At second years densities changed with 2000 and 2500 ind./hec. and each of traetments divided fishes with average primary weights of 80 and 115 grames. The fries and fish feeding begins in April when the water temperature rise above 18 °C and it cut in fall when the temperature goes under 20°C. They fed twice a day at 8 AM and 2 PM and the amount was 5-7 percents of the fish existent biomass in each ponds. They fed with dry formulated feed made of Mahdaneh Karadj Company (Danso dry feed) both the first and second years of farming. The feed prepared for carps feeding and containded 23±2 % crude protein, 10 ±2 % crude lipid, about 7 % crude fiber, 15 % ash, 40 ±2% carbohydrates and with energy content of 4000±200 kcal/kg. After seven months culture period the average weight and length of fish in treatment 5000 ind./hec. were 113.7 gr and 21.1 cm respectively and in treatment 10000 ind./hec. the average weight and length were 86.6 gr and 19.6 cm respectively. At first year the average survival rate in different treatments was 77 percent. The average FCR in treatments 5000 and 10000 ind./hec. were 3.4 and 3.9 respectively. At second years after six months culture the average weights in pond no. 9 (with primary weight 115 gr and density 2000 ind./hec) , pond no. 10 (with primary weight 80 gr and density 2000 ind./hec) , pond no. 11 (with primary weight 115 gr and density 2500 ind./hec) and pond no. 12 (with primary weight 80 gr and density 2500 ind./hec) calculated as 476.6 ,338.1, 366.5 and 440 gr respectively. The average survival rate and FCR were 93 % and 3.1 respectively. Ponds no. 9 and 11 (fish stocked with primary weight 115 gr in densities both 2000 and 2500 ind./hec) selected as the best economic efficient treatments.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Barcodes are short segments of DNA that can be used to uniquely identify an unknown specimen to species, particularly when diagnostic morphological features are absent. These sequences could offer a new forensic tool in plant and animal conservation especially for endangered species. Ideally, barcodes could be used to positively identify illegally obtained material even in cases where diagnostic features have been purposefully removed or to release confiscated organisms into the proper breeding population. In the present study molecular data were used besides the morphological ones to introduce a specific barcode for six selected species of Shrimp. Based on the previous phylogenetics study, it has been proved that the mitochondrial COI gene in crustacean is a good discriminative marker at both inter- and intra-specific levels. In this regard, six species of shrimp (Fenoro penaeus indicus, Fenoro penaeus merguensis, penaeus semisulcatus, Metapenaeus affinis, Marsupenaeus japonicas, Fenoro penaeus penicillatus were collected from the Persian Gulf and Oman sea. After identification, the total DNA was extracted; COI gene was first amplified and then sequenced for each species. Finally the collected data were analyzed with the specific phylogenetic software. Molecular analysis revealed some degree of interpopulation differences but six good species were recognizable based on COI sequences. Also for population study molecular data of two species Penaeus semisulcatus and Metapenaeus affinis were analysed base on COI RFLP and 16SrRNA sequences respectively. The results would be helpful to protect shrimp species.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The effects of added artificial substrates on juvenile white Indian shrimps (Penaeus indicus) were evaluated. .....

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The fishery stocks of most commercial aquatic stocks in the world have shown declining trends in the past two decades. Several factors have been responsible for the decline of stocks the most important of which over fishing and over-exploitation, pollution, loss of natural habitats and natural spawning grounds, construction of dams and bridges across the important rivers which restrict the migratory routes of spawners, decrease in natural reproduction and rehabilitation of stocks through artificial breeding programs. Over-exploitation of stocks and pollution directly affect decreasing stocks in an ecosystem. Not differentiating between different populations and stocks of a species found distributed in an aquatic ecosystem is considered one of the main factors which causes the depletion of stocks in most ecosystems in the world. In most cases this is because genetic variations in aquatic stocks in the wild are not taken into consideration. Six species of sturgeons are found living in the Caspian Sea and its drainage basin which produce more than 85-90% of the world caviar. The Persian sturgeon (Acipenser persicus) and the stellate sturgeon (Acipenser stellatus) are the main sturgeon species of the Caspian Sea. The Persian sturgeon is mainly found in the south Caspian Sea while the stellate sturgeon stocks are considered shared stocks by the five Caspian littoral states. Due to over fishing in the past two decades the legal catch figures for sturgeon stocks in the Caspian Sea dropped from 28500 tons in 1985 to less than 1500 tons in 2004. Similarly caviar production also dropped from 3000 tons to 110 tons in 2005. With regard to the severe reduction in sturgeon stocks it is necessary to take essential steps before these valuable species are totally wiped out. The fisheries management of the five Caspian littoral states should focus their efforts on identifying the different populations and stocks found in the Caspian Sea. Concerted measures should be taken to study the distribution and biomass of the different populations in order to develop a scientific solution for the sustainable use of these endangered species and to secure the long term conservation of sturgeon stocks. The aim of present study was to evaluate the genetic structure of the population of two species Acipenser persicus and Acipenser stellatus and to develop molecular markers to identify and differentiate different populations of these two species.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The aims of this project was to inform and aware about the red tide condition before entering the algal bloomer in water resources to the hatcheries and farmed shrimp complexes in Bushehr province coastal. Field investigation and sampling have been carried out in the southern of the input water channels of Mond, Delvar and Helleh farmed shrimp complexes, which are located along the coastal of Bushehr Province, from April to December 2011 .The physic-chemical parameters such as pH, salinity and water temperature and also meteorological conditions were measured and recorded on the field. The water sampling has been lunched for determination of nutrients and chlorophyll - a, phyto- and zoo- planktons. No Cochlodinium.sp outbreaks have been detected in the studied area, during the study. The only bloom which reported by a fisherman, was outbreak in offshore of Bushehr province in Khoure Khan on 13th September 2011. The sample which transferred to the Iranian National Shrimp Research Institute was included Alexandrium.sp and its density was 2 million cells per liter. The identified phytoplanktons were belonged to three order of Bacillariophyceae (52.6%) with average density of 10778 cells per liter, Dinophyceae (37.7%) with density of 7731 cells per liter and Cyanophyceae (9.7%) with density of 1980 cells. 12 genera belonged to Dinophyceae , 25 genera of Bacillariophyceae and two genera of Cyanophyceae were observed during the study. The highest density of phytoplankton was recorded in Helleh station by 18374 cells per liter. The maximum density of phytoplankton was at Delvar station by 141120 cells per liter in December. The highest density of the phytoplanktons was belonged to Dinophyceae by 126000 by cells per liter of which the Alexandrium.sp had the density of 124500 cells per liter in August 2011. From the Dinophyceae the Alexandrium.sp with mean density of 20345 cells per liter, Ornithocercus 920 cells and Prorocentrum.sp 820 cells were the predominant species. The identified Zooplankton in were belonged to 8 branches and 19 groups. The highest density of zooplankton was recorded in Helleh station by 1194 no. per liter. Nauplii were the dominant zooplankton groups with an average density of 136.4 no. per liter, Tintinnids 98.7, Cyclopoida 60.8, Calanoida 35.7 and Harpacticoids 14.5 no. per liter . The average of water and air temperature was recorded 29.4 °C and 28.3 °C, respectively. Average of salinity was 41.2 and pH was 8.46. The average of depth in all stations was 5.7 m. The mean concentrations of silicate, nitrate, nitrite, ammonia and total phosphate were 1.99, 0.03, 0.009, 0.14 0.15 ppm, respectively and the average of chlorophyll - a was 0.94 mg.m-3.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Study of survey health management and diseases in hatcheries and fish farms can help us to knowledge and application control methods such as: prevention, treatment and increase high levels of production in hatchery and farms, finally. This survey carried out from 2005 to 2008 for 4 years in sturgeon hatcheries and farms of Golestan province. Sturgeon fishes include Huso Huso, Ship sturgeon, Acipenser persicus collected and for virology, bacteriology, fungius and hematology examined. Also, physicochemical parameters measured and recorded in different stages of culture. Results of this study showed that all of samples in virology was negative and did not observe any doubetful causes. In bacteriology CFU was variation from 3/9 ×105 to 6/9×10. The most parasites that detected in this survey was Cocolanus espherolanus , Sceria binopsulus semiarmatus and Amphilina fuliacea that separates from Acipenser Percicus, especially. The results about hematology parameters some important hematological indices of ship sturgeon include: The total RBC for female and mail specimens measured as 5.3±1.5 ×105, 4.8±0.5 ×105 per mm 3 respectively. The amount of haematocrit and hemoglobin for female and mail determined: 34.3±2.8, 35±1.4 percent and 10.3±0.9, 8.9±0.8 gr/dl .The MCV: 216.3± 96.2, 736.5± 102.5, MCH: 720.2±309.5, 186±0.7 and MCHC: 30±0.8, 25.5±3.4 percent respectively.The total WBC were (female, male): 21320±1054, 20580±777 per mm 3 and neutrophil: 16.4±2.5, 17±1.4 percent and lymphocyte: 74.4±2.4, 73.5± 0.7 percent and eosinophil: 6±1.4, 6.4±0.5 percent, monocyte: 2.8±0.8, 3.5±0.7 percent. There was not any significant differences (p〉0.05) between mentioned parameters in male and female (students t-test). Also evaluation of hematological parameters in bluga ( Huso huso) include: total RBC were (male , female) 5±0.3 ×105 , 4.9±0.6 ×105 per mm 3 ,respectively and hematocrit: 33.2±6.7 , 35.4±3.4 percent and hemoglobin: 11.2±1.5 , 12.2±1gr/dl and MCV: 669.9±172.2, 723.9±982.4 and MCH: 226.2±42.5, 249.5±35.4 and MCHC: 34.1±2.4, 34.6±3.6 percent respectively. The totals WBC were (male, female): 24800±707.1, 23042±1375.4 per mm 3 and neutrophil: 18.5±0.7, 21.4±1.1 percent and lymphocyte: 73.5±1.4, 68.4±1.1 percent and eosinophil: 5±2.8, 7±1.2 percent and monocyte: 3.5±3.5, 3.2±0.8 percent. According to statistically study the count of lymphocyte had significant difference between male and female fish and this count in male was higher than female. (p≥0.05).

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This investigation conducted for evaluation Silo (Cylindrical) Fish Tanks as an applicable method for aquaculture of Rainbow trout (Oncorhynchus mykiss). Current research was designed in two practical phases as accidental experiments through 3 treatments and repeats of which stocked by three Rainbow trout (375 gr) densities as 75, 100 and 125 fish/m3 in 9 silo fish tanks (2 m3). For better understandings of the silo performance method, concrete circular fish tanks (similar volume) considered as controls and stocked as similar fish densities as silo tanks. The duration of the first phase of experiment was 150 days. The second phase of the experiment was determined for evaluating two proposed silo type (Inlet and outlet in bottom side / Inlet from bottom and outlet in upper side). In this stage, proposed silos (stocked by fish of 294 gr weigh) tested for rearing trout for 30 days. Water supplied from two surface wells after some aeration operations. Stocked fish were fed by pellets made by Fish Feed Chineh Company (Ltd) in Iran. Feeding operation conducted according to the recognized manuals by consideration of water temperature, fish weight and biomass. Meanwhile, biometry operation conducted in 10 days intervals while fish were anaesthetized. In addition, water quality was determined by daily measurements. According to the results of the first phase of experiment, there were significant variations on Growth rate, survival rate, FCR, FCE and SGR through the cultured organisms in silo tanks compared to the circular tanks. Meanwhile, according the results of an economic study on this survey, the fish culture in silo tanks showed significant economical and profitable in compare to controls. In additions, the second phase of experiment showed better aeration conditions in silo tanks when inlet water supply from bottom side and outlet located in upper side. Because of the fine distribution of fish in silo tanks and more usage of depth by this method, fish culture would be an applicable and profitable technique in narrow lands and slopes through the mountains area. The maximum stocking rate recorded more than 100 Kg fish/m3 in silo tanks.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Neogobius caspius is a small benthic fish that is native to the Caspian Sea. The importance of this fish is because of it is role as a main food resource of the Sturgeon fish. The genetic diversity of N.caspius populations in the Caspian Sea was studied using microsatellite technique. In the study 95 Samples of Neogobius caspius from two regions (torkman port and Chalus) in south Caspian Sea were collected. DNA was extracted using 12 pairs of microstellite primers polymerase chain Reaction (PCR) was conducted. DNA Bands were analyzed using uvdoct and GenAlEx software package. Out of 12 microsatellite primers, 11 loci were produced, in which 5 of them were polymorphic ,6 monomorph and one of them showed smear. Average of observed and expected hetrozygosity was 0/798 and 0/843 respectively. Significant genetic differences between 2 regions was observed (P 0/01). Deviations from hardy- Weinberg equilibrium were in all cases. These results indicate that at least, 2 population of Neogobius caspius exist in the south Caspian sea.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Study of Mahisefid population using mtDNA with PCR Mahisefid diversity was studied in four rivers including Lamir, Sefidrood, Shirrood and Tajan. Total sample was collected from four rivers when fish migrat to river for spawning, 100 samples from Sefidrood, 98 samples from Lamir, 48 samples from Shirrood and 48 samples from Tajan. DNA was extracted with phenolcholorophorm. Samples were used for RFLP, the PCR product were digested by 20 restriction enzymes as follow: TasI, HaeIII, HinfI, HincII, SalI, DraI, AccI, AvaII, XhaI, BshNI, AvaI, BclI, BshII, MspI, PstI, RsaI, SdnI, TaqI, TruI, VspI. The four restriction enzymes including: TasI, HaeIII, HinfI, HincII showed diversity, 6 enzyme didn t have any restriction and 14 enzyme showed monomorphic. Total 20 haplotype studied that haplotype AAAA and BAAA had most frequency. The average haplotype frequency of AAAA was 29.93% and the average haplotype frequency of BAAA was 27.55%. 2- Study of population of Mahisefid with microsatellite markers 120 specimens of R.frissi kutum were used from four rivers to test thirty primers (30 samples from each river) of which 8 primers showed polymorphism. A large variation in heterozygosity average over all samples was observed among loci, that ranged from 0.13 to 0.91. For a given locus, observed heterozygosity varied greatly among the samples. For example, in Lamir 0.07 at SPY5 and 1 in Sefid Rud at CA1. Tajan had an observed heterozygosity of only 0.17, whilst the Lamir had an observed heterozygosity of 0.07 at locus SPY5. At locus SPY4, Tajan and Sefid Rud are the same and (0.53) and Shir Rud is 0.83 and Lamir is 0.47. However, despite these differences, there was clear difference in average heterozygosity observed between the samples. To investigated of Hardy-Weinberg Equilibrium in all locus and all rivers deviate showed from Hardy-Weinberg Equilibrium was significantly different ( P〈0.01)except in SPY6 locus. The maximum average Ho was 0.91 in locus CA1 and minimum was 0.13 in locus SYP5 and maximum average of He was 0.89 in locus SYP4 and minimum was 0.28 in locus SYP6. Highest Ho was 1 in locus CA1 in Sefid Rud River and lowest was 0.07 in locus SYP5 in Lamir River , highest He was 0.92 in locus SYP4 in Shir Rud River and lowest was 0.24 in locus SYP6 in Tajan River. According to Nei (1978) the highest genetic distance was between Shir Rud River and Tajan River (0.07) and lowest genetic distance was between Sefid Rud River and Tajan River (0.05). The highest genetic similarity was between Sefid Rud River and Tajan River (0.95) and the lowest genetic similarity was between Shir Rud River and Tajan River (0.93). FST was significantly different (P 〉 0.05) between all samples except Lamir River and Sefid Rud River. The highest Fis was in Lamir River (0.84) in locus SPY5 and in Sefid Rud River in locus SPY5 (0.71), in locus CA12 (0.67) in Shir Rud River and SPY5 (0.63) in Tajan River. The lowest Fis is in locus CA1 (-0.55) in Sefid Rud River, in locus CA1 (-0.47) in Tajan River, in locus CA1 (-0.28) in Lamir River and in locus SPY6 (0.16) in Shir Rud River.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This investigation carried out for the first time in Iran inorder to prodcution of monosex female and also sterilization in Rainbow trout. In this study, the eggs of general females were fertilized with the sperm of sex reversed male and so monosex female population was produced in second generation and sterilization carried out with oral administration of 17α methy 1 testosterone and immenrsion and oral administiration methods were used in embryonic stage and from commencing of acitve feeding of larvae, respectiverly. For sex reversal , 13 treatments were considered totally, that the most percentage of male (100%) was observedc in a treatment including of orally administration of 0.5 ppm hormone for 60 days after commencing active feeding (P〈0.001). In the other treamtnet, different percentages of sex ratio including male, female, intersex and sterility were observed. The offspring of genral eggs fertilization with the sperm of masculinized fish were 100% female, chisquare test was shown the treatment of orally administration of 30 ppm hormone for 120 days after commencing active feeding that had been considered for sterilization, was produced 90% sterile fish (P〈0.001) and was changed the sex ratio significancthy. Morphological changes of the gonads and sperm ducts in matured fish and also histological changes in the gonads of fish in the treamtints were considerable.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This project was carried out in order to increasing of nutritional value, taste and shelf life of cleaned Kilka Fish during cooled storage. Edible films made by Whey protein and Sodium alginate were used for fish packaging. This search carried out in two stages consisting of pre- study and study. 3, 6, 9 and 12% concentrations of Wp and 0.5, 1, 1.5 and 2% concentrations of SA at three times including 0, 2 and 4 hours were used in pre-study stage. The covered samples were kept in -18 ֯C. Microbial and sensory examination were carried out for a period of two months. Microbial factors were including total bacterial count, Staphylococcus bacteria count, Coliform, Escherichia coli and Pseudomonas bacteria. Sensory tests consist of taste, odor, color and tissue were studied in the fish samples. 12% and 0.5 % concentrations at time = 0 of edible films made by WP and SA considered in study stage. This is can be due to the significant differences in total acceptance index of sensory tests. Control sample cleaned Kilka was packaged in disposable dishes with cellophane covers in 500gr in weight. Two selected timar and mixed cover including 12 % and 0.5 % concentrations at time = 0 of edible films by WP and SA considered in study stage. The covered samples were kept in -18 ֯C. Microbial, chemical and sensory examination were carried out for a period of six months. These factors and chemical factors consisting of humidity, protein, lipid, ash, calorie, Peroxide value, free fatty acids, thiobarbitoric acid, TVN and pH were studied in test samples compared with the control samples. Coliform, Escherichia coli and Pseudomonas bacteria contamination were negative until the end of storage period in the covered samples. The mean total bacterial count and Staphylococcus bacteria counts in processed samples by WP were 2.47 and 1.61 logcfu/g, in processed samples by SA were 2.84 and 1.28 logcfu/g, in processed samples by WPSA were 2.51 and 1.44 logcfu/g, and in control samples 4.11 and 2.93 logcfu/g from 1 day until six months after processing, respectively. The mean of moisture, peroxide value, TVN, pH, free fatty acids, thiobarbitouric acid , protein, fat, ash and calorie in the covered samples by WP were 73.91%, 0.13 meq/kgoil, 9.84mg/100g, 6.15, 1.15gr/100, 0/006 mg/kg, 19.00%, 4.25%, 2.1% and 120.73 kcal/kg, in the covered samples by SA were 73.91%, 0.06 meq/kgoil, 9.84mg/100g, 6.15, 1.15gr/100, 0/006 mg/kg, 18.85%, 4.72 %, 1.90 % and 125.98 kcal/kg, in the covered samples by WPSA were 73.91%, 0.06 meq/kgoil, 9.84mg/100g, 6.15, 1.15gr/100, 0/006 mg/kg, 18.50 %, 4. 65 %, 2.25 % and 126.48 kcal/kg and in control samples 59.43%, 3.25 meq/kgoil, 16.22mg/100gr, 6.71, 9.21gr/100, 0/15mg/kg, 18.2%, 4.00%, 1.80% and 107.10 kcal/kg, respectively. No statistically significant differences were observed in the results of chemical experiments of the covered samples and presence of the meaningful difference at the results of the chemical experiments of the control sample, The covered samples by WP, SA and WPSA up to the end of storage period at cold-room had a favorite quality but the control samples had lost their. No statistically significant differences were observed in the WP samples compared with the WPSA samples (p〉 0.05). Samples covered by SA had better quality compared with other samples which can be due to the presence of the significant difference in total acceptance index among covered samples without considering of economical worth.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In order to perform the project, 446 samples of rainbow trout (Onchorhynchus mykiss) from 24 different regions in Iran were collected. About 2-3 g of caudal fin samples was collected from each specimen and preserved in absolute ethyl alcohol and then transferred to the genetic laboratory. Genomic DNA was extracted using the phenol-chloroform method and then DNA content and quality was determined using spectrophotometry and agarose gel electrophoresis, respectively. Polymerase Chain Reaction (PCR) of genomic DNA fin samples was carried out using 10 pairs of microsatellite primers. All PCR products were electrophoresed on 6% polyacrylamide gel and stained with silver nitrate. Following the scoring of alleles, all parameters including allelic frequency, effective number of allele, observed and expected heterozygosity, shanon index, measurement of similarity and genetic distance and Hardy-Weinberg equilibrium, Fst , Rst and gene flow were calculated using AMOVA analysis in the GenAlex and Popgene programs. The results showed that 8 pairs of microsatellite primers were polymorphic. In total, 50 alleles were determined with the range size of 64-280 bp. The locus omyf had maximum number of allele (26) and loci OTSG 474 and Strurruta58 had minimum number of allele (5). The observed heterozygosity was between 0.86 and 0.964. Hardy-Weinberg departure was observed for all loci from farms 18, 15, 4, E20 and 21 and were disequilibrium (P〈0.05). The farms 14, 8, 7 and 6 were equilibrium at 3 loci, but showed disequilibrium in other loci. The other farms were equilibrium at 1 or 2 loci and disequilibrium at 8 or 9 loci. The FST results showed that maximum FST (0.24) were between farms 1 and 11in which had minimum of gene flow (3.7). Minimum FST (0.04) were between farms 8 and 9 in which had maximum of gene flow (346). Based on the results of AMOVA analysis, significant differences were detected between all farms (P〈0.01). Furthermore, based on Nei 's standard (1972) maximum genetic distance (0.89) were observed between farms 2 and 11 and maximum genetic similarity (0.15) were detected between farms 3 and 4. This result suggests that the unique genetic variation of rainbow trout in hatchery farms of Iran represents a highly valuable genetic resource and provide useful information for creating a based population in the future breeding programs.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The effects of aquaculture industry on the environment were evaluated by studying the water quality of Bushehr costal water during culture season 2005 and 2006. The variations of selected parameters such as total phosphorus, ammonia, chlorophyll a, pH, salinity and... Were monitored in effluent canal, influent canal and open sea in Helleh and Mond regions as monthly. Following occurrence of White Spot Disease (W.S.D) in the shrimp ponds of Bushehr province, shrimp culture suspended and entrance of effluent waters completely were blocked since August 2005, despite the absence of aquaculture sewage evaluation of environment and sampling were continued. During releasing of sewage of shrimp ponds to the coastal waters of Bushehr in Jun 2005, the average amount of ammonia in Helleh and Mond were obtained to be 0.161 mg/l and 0.194 mg/l, however the average amount of total phosphorus in Helleh and Mond regions were 0.149 mg/l and 0.043 mg/l, respectively. Although effluent water suspension, amount of ammonia and total phosphorus were increased in both region especially in June 2006. Comparing present data in Jun 2005 and recorded data from culture period 1997-2003 with permitable range of municipal and aquaculture waste show that; aquaculture industry on both regions have not had any obvious negative effect on the coastal waters of Bushehr but it seems if other sources of pollutions in the region is not controlled, then the aquaculture industry can contribute to occurrence of environmental problems.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In 22 August of 2000 an adequate amount of fresh cow manure was transported form Golshahr Cow Farm and deposited in the experiment site. The transported manure was divided equally between five treatments of siloes, depots without plastic coverage, depots with plastic coverage, barrels and tubes. Deposited manures retained in the treatments till 20 May 2001. During the retention time, sampling from three points of cow farm and other treatments carried out with monthly intervals and samples were analyzed for determination -of Ca, Na, NDF. ADF. crude protein, crude fat, moisture; crude fiber, minerals, dry mater and NFE. Temperature, pH and redox potential were measured in situ and all data were analyzed statistically by SAS general linear models. The results showed that month and treatment exert significant effects (P〈0.01, on the variations of the most measured parameters. Depots with plastic coverage had highest mean temperature and then there were depots without plastic coverage, siloes and barrels, respectively. “The temperature increased significantly in the first month of retention but showed some fluctuations in subsequent 4-5 months and then remained relatively constant till the end of experiment period. Depots without plastic coverage showed highest moisture. Optimum moisture value of 40-50 percent obtained for siloes and depots with plastic coverage. Lowest Ca and highest Na values were measured in fresh manure of the cow farm. In two first month of retention time NDF decreased significantly in most treatment but increased subsequently and reached maximum 'percentages during 5-6th month of retention. In first month of retention ADF showed significant decrease in most of treatments but increased in further months and reached to maximum percentage after 4 month retention. In siloes, depots without plastic coverage, and depots with plastic coverage the percentages of protein and fat reduced significantly in the first month of retention, modified during subsequent 6 months and reach valuable percentages. Depots with plastic coverage showed maximum and most suitable protein and fat percentages of about 9 and 2.75 percent after 6 months retention. The percent of crude fiber decreased to minimum level after 3, 5 and l, retention months in siloes, depots without plastic coverage, and depots with plastic coverage and barrels respectively. Values of surface pH in siloes, depots without plastic coverage vice versa of other parameters were minima of 7.1-7.5 at the beginning of retention time that reached to 7.8-8.4 after 4 retention months. The pH of cow farm mostly was more than other treatments. Amount of redox potential in cow farm, siloes, depots without plastic coverage, depots with plastic coverage and barrels fluctuated between -48 to -109, -84 to -12, -62 to -22, -86 to -30 and -65 to -21 millivolt. The minimum levels were at beginning of period and the maximum levels obtained after 4 months of retention in treatment I and 2, after 3 months in treatments 3 and after 5 months in treatment 4. Therefore with care to extent and terns of parameters changes in variation treatments, depots with plastic coverage can introduce the best retention conditions during 4-6 months for decomposition of organic manure for usage in aquaculture.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Recently, the development of aquaculture has focused on the use of seawater, because of freshwater crisis in the world. Whiteleg shrimp (Litopenaeus Vannamei) is one of the most important species for aquaculture development, because of tolerance and adaptation to different ecological conditions. One of the advantages of species in aquaculture, compatibility status is dense in the rearing period. In this study, the effect of different densities (45/m2 , 50/m2 , 55/m2 and 60/m2 ), of primary stock whiteleg shrimp postlarvae (PL12) on the growth and survival rate were evaluated. Brackish water (10.52±0.43 ppt) was providing from Caspian Sea. The experiments were performed in 12 circular concrete pond with a sandy bed (area: 78m2 ) in four treatments and three replications for each treatment. The experiments performed in a 75-day period. In this study, the water temperature was 27.4±1.79°C. The results showed statistically difference in growth parameters and survival rate among experimental treatments (Duncan test, P〈0.05). Therefore, with high levels of density, has decreased the amount of weight gain and survival rate (SR), specific growth rate (SGR) and average daily growth (ADG). In addition, the treatments were different variations of FCR (P〈0.05) and not depend on the primary stock density of postlarvae. In low density (45/m2 ) were observed the highest growth (SGR=11±0.04 and ADG=0.25±0.01 gr/day/ind.), survival rate and calculate the amount of production per 78m2 equal 43.6±3.3kg (5596±433kg/ha). Therefore, It is possibility that there is commonly increasing primary stocking of density about L. Vannamei postlarvae culture in Iran.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The population genetic structure of five Caspian Sea sturgeon species was investigated. Totally 1121 samples of caudal and unault's fin tissue of the sturgeons (Acipenser persicus, A. gueldenstaedtii, A. stellatus, A. nudiventris and Huso huso) were collected from the Volga River (Russia), Ural River (Kazakhstan), Kura River (Azerbaijan), Sepidrud River and the coastline of the south Caspian in the Iranian waters as well as from the sampling stations selected for the marine survey for sturgeon stock assessment in the Caspian Sea. All samples were stored in 96% ethyl alcohol and transferred to the genetic laboratory of the International Sturgeon Research Institute. Genomic DNA was extracted using phenol-chloroform method. The quality and quantity of DNA was assessed by Agarose gel (1%) electrophoresis and spectrophotometry. The population genetic structure of Ship and Persian sturgeon was studied using both PCR-RFLP (D-loop and ND5/6 gene) and microsatellite technique and that of H. huso, A. stellatus and A. persicus were studied using microsatellite technique. After amplification of genes using PCR, the RFLP technique was used to digest mtDNA using restriction enzyme. The PCR products were electrophoresed on 6% sequencing polyacrylamide gels followed by silver nitrate staining. Data for PCR-RFLP were analyzed using REAP program and those from microsatellite technique were analyzed using Gene Alex. Population genetic parameters including allele frequency, expected and observed heterozygosity, effective allele, Shannon's index were determined. Genetic identity and distance were calculated following Nei criteria and Hardy Weinberg equilibrium was tested based on X2 and analysis of molecular variance (AMOVA) using Reap and Gen Alex at 99% confidence limit. Phylogenetic relationship was determined and drawn using TFPGA program. The population genetic structure and genetic diversity of the 1121 sturgeon specimens were determined. Three independent populations were identified for Acipenser persicus (two populations in the south Caspian in the Iranian waters and one in the north Caspian). Three independent populations were identified for A. gueldenstaedtii (Volga, Ural and South Caspian populations) using the microsatellite technique. Population genetic structure using PCR-RFLP revealed no genetic differentiation among the A. gueldenstaedtii specimens studied from the different regions using ND5/6 gene, while two populations (Ural and south Caspian populations) were detected for this species with the same technique using D-loop genes. Four independent populations (Volga, Ural, Kura and Sepidrud populations) were reported for A. stellatus using the microsatellite technique and four more populations which most probably belong to the autumn and spring races of the above mentioned independent populations were identified for this species. The present study also identified two populations for H. huso; The North Caspian population (in Volga and Ural Rivers) and The South Caspian population (in Golestan and Guilan regions) which were significantly different from each other (P〈0.01). The genetic population structure of A. nudiventris was studied using the microsatellite and PCR-RFLP techniques which revealed two populations for this species one in the Ural River and the other in the Sepidrud River (South Caspian). Comparison of the ND5/6 and D-loop genes studies in Russian sturgeon revealed that the D-loop gene is better than the ND5/6 genes in population's differentiation and is therefore strongly recommended for population genetic studies on sturgeons in the Caspian Sea. Genetic diversity studied using microsatellite technique was higher and more accurate as compared to that using RFLP. Nevertheless the RFLP technique was able to introduce molecular markers for the population’s species pacific identification. On developing suitable primers these studies can be speeded up and the cost of such studies can be cut down. However the drawback in using microsatellite technique for population genetic studies is that it cannot introduce a molecular marker for the identification of populations. The present study was able to introduce molecular markers to differentiate the ship sturgeon population in the south Caspian from that in the Ural River using the PCR-RFLP technique Based on the results obtained it is strongly recommended that all activities related to restocking and rehabilitation of sturgeon stocks in Iran be conducted on the basis of genetic principles. Also serious and immediate measures should be taken for the restoration and conservation of rare population of native species of Iran particularly in the Sepidrud region using genetic markers before they are become extinct.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The two groups of grass carp, have been propagated in North and South fish farms and then the larvae were transferring to Abziroshd and culture fish farm of Mazandaran. The fingerling at eight weight classes of 2 to 500 gram were investigated morphologically and some morphometric and meristic were compared. The South grass carp was significant difference (P〈0.05) with the North, in case of weight with the same length, number of vertebral and gill raker. The coefficient of variance (CV) of meristic characters of South Grass carp was in the range of 1.2 to 9.8 while in Mazandaran grass carp 2.8 to 8.6. This CV illustrate the low variance in both population. This research study showed that the two stocks can be regard as difference races from one ancestor.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Without Abstract.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: For the first time white spot disease (WSD) was reported in shrimp farms of khoozestan province, in southwest of IRAN in 2002. Then in 2005 the neighbor province, boushehr, was contaminated. In 2008 WSD outbreak reported in sistan-bloochestan province in southeast of Iran. In 2015 all of southern shrimp farms of country except Hormozgan, the middle southern province, which has remained free of WSD, are being contaminated. White Spot disease suspended shrimp culture in thousands hectares of shrimp farms. Considering that white spot disease has not been observed in Hormozgan province yet, the question is; to what extent environmental and management factors participated in preventing WSD outbreak or cause WSD outbreak. In this study (20102012), the effects of environmental factors and management, stressors that decrease immune system function of shrimp are discussed. In addition, the role of pathogen as the main factor of outbreak is discussed. The goal of this study is to define environmental parameters and management practices associates with outbreak of white spot disease in affected provinces and discover reasons of being Hormozgan province free of this disease. In this study the role of the local environmental factors and management practice stressors in susceptibility to WSD was determine. Both the effects of environmental factors in water of ponds including total ammonia, nitrogen, dissolved oxygen, pH, salinity, transparency, and temperature and management issues related to biosecurity are studied. There were overlaps on physical and chemical parameter values obtained in clear areas with contaminated areas .Results of the data analysis suggest that lack of association with WSD incidence was 7 times greater than WSD incidence despite of disease outbreak in sistan-bloochestan province, so other sources of white spot disease virus incidence was suspected in affected areas. Histopathological examinations and polymerase chain reaction (PCR) tests during project performance did not reveal white spot disease virus evidences in post larvae examined from khoozestan province stocked in farms but disease outbreak was happened in that farms , so we suspected to management practice include feed , pond preparation and carrier of disease . Recorded values of temperature and salinity in some months during inspection in Hormozgan province specified stressful condition that may lead to WSD outbreak, however the disease did not appear. Therefore the hypothesis that the water physical and chemical conditions are reasons to prevent disease outbreak in Hormozgan province is being rejected. The policy of Hormozgan’s fishery authorities, to replaced Fenneropenaeus indicus with specific pathogen free Litopenaeus vannamei, that is more resistant to some of diseases, before incidence of WSD in farms and to before being endemic in the Hormozgan province, made an advantage compare to affected southern provinces that introduced Litopenaeus vannamei after WSD prevalence to their farms. However it does not guarantee to maintain current trend of being Hormozgan province farms free of white spot disease. Therefore establishing the principals of biosecurity are strongly emphasized. Strategies taken by the proficient authorities in preparation of SPF shrimp broodstock can be the most important factor in preventing WSD. Regarding biosecurity principals purchased feed must be free of shrimp head powder. Construction the new shrimp farms should be as far as it could be away from contaminated areas.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The project of "Assessment of different methods of water treatment on decreasing pathogenic bacteria in shrimp hatcheries" achieved by mean of reviewing the effectiveness of various methods on the water treatment to reduce bacterial load of sea water. The project performed in Bandargah region of Bushehr province in order to checking the effect of different methods on the sea water and bacterial population. This project has been done over a year. In this study a total of 138 samples was collected during seven times and was evaluated as well as 7 treatments. In this study, the comparison of the effect of each factors (sand filter, chlorine, ultra violet ray, chlorine combined with ultra violet ray) was analyzed on the sea water by means of disinfection. During this study, other environmental factors such as water temperature, dissolved oxygen, pH and salinity was measured till we can ensure that they don’t have any negative impact on the treatments. Results showed that the density of total bacteria in sea water (Control) was averagely 5187 CFU/ml, this was done while the total density of bacteria in the sea water, reservation pool sample, sand filter water, water that irradiated with ultraviolet rays, chlorinated water and the water that was influenced by both chlorine and ultra violet ray was respectively 5187 2137, 4449 2042, 2782 1335, 1164 692, 143 104, 87 76 CFU/ml. Therefore, reservation pool, sand filter, ultra violet ray, chlorine, chlorine-ultra violet ray each of them was reduce respectively 14, 46, 77, 97, 98 percent of the density of total bacteria or in other words they reduced 0.06, 0.27, 0.65, 1.56, 1.77 log of the total population of bacteria in water. Moreover, the results from the total count of vibrios showed that reservation pool, sand filter, ultra violet ray, chlorine, chlorine-ultra violet ray each of them was reduce respectively 17, 47, 74, 98, 99 percent of the density of total vibrios or in other words they reduced respectively 0.08, 0.27, 0.59, 1.66, and 1.95 log of the total population of vibrios in sea water. Also the bacteria that isolated from each treatment were identified with the use of biochemical methods that totally were includes a wide range of bacteria, the identified vibrios were: Vibrio alginolyticus, V. costicola, V. fischeri, V. fluvialis I, V. fluvialis II, V. harveyi, V. natriegens, V. nigripulchritudo, V. parahaemolyticus, V. plagius I, V. vulnificus, V. anguilarum I, V. campbellii, V. nereis, V. proteolyticus, V. splendidus, and Unknown V. spp. Other identified bacteria belonging to the following genus: Acinetobacter, Aeromonas, Alcaligenes, Bacillus, Borkholderia, Eschricia, Enteroacter, Flavobacterium, Micococcus, Proteus, Pseudomonas, Serratia, Staphaphylococcus, Streptococcus, and Unknown Bacteria spp. By comparing the results, we concluded that the best result was related to treatment that the sea water had been disinfected after the combined effects of chlorine and ultraviolet ray. It was determined that this process in compared with other treatments, had been reduced the total number of bacteria, the total number of Vibrios, the number of sucrose fermentative vibrios, and the number of non-sucrose fermentative vibrios respectively 98, 99, 98, 99 percent. In other words, the number of bacteria was reduced respectively 1.77, 1.94, 1.82, 2.23 log. So in this study, the applied method was introduced as the most effective way of disinfecting sea water. Meanwhile, none of pathogenic bacteria for shrimp including Vibrio harveyi was isolated by using this treatment. Comparing the results showed that the combined effects of chlorine and ultraviolet radiation is the most effective applied method for disinfecting sea water.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Aquaculture is the most important part of Fisheries Organization duties in Iran. During several planning and developing programs, aquaculture productions had a considerable growth. Due to good potential, these productions were higher than the planed one. The North Alborz regions were investigated by four groups of experts. The regions which investigated were including 3 important provinces (e.g. Guillan, Mazandaran and Golestan). The area where investigated was 940 km along with coastal zone of Caspian Sea or 58678 km2 in total. Around 27% of aquaculture production came from this region. However, subdivision of fisheries can play important role in the economics in the region. The fisheries sub-sector has an important role in the economy of the region, and despite the great potential for agriculture and tourist industries in the North Alborz, there is a significant role in the prosperity of the fisheries activities. In the present study shows that Mazandaran and Guillan provinces has more potential in reservoirs and aquaculture production when compared with other provinces. Productions in Mazandaran either in reservoirs were less than Guillan province. Due to lee in reservoirs, Mazandaran had less investment when compared with Guillan province. The figures show that carrying capacity of aquaculture production for 3 provinces (Mazandaran, Guilan and Golestan) was higher than other provinces in the entire country. Due to, large land base, less investment, more interesting of farmers, no needs of complicated technology, farmer would be able to develop reservoirs as consequences to enhance productions. However, developing reservoirs and supporting of it's; can increase the contribution of warm water species in inland water.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In order to obtaine live individuals of cobia ( Rachycentrun canadum ) , coastal waters of Jask, Sirik, Kolahi , Bandar Abbas, Bandar-e Lengeh ,Bandar-e Bostaneh and Abu moosa ,Hormoz and Qeshm Islands have been surveyed from March 2008 to January 2010 in Northern waters of Persian Gulf (Hormozgan province). All in all 60 alive fishes obtained from fishing boats and transferred to Aquaculture Department of Persian Gulf and Oman Sea Ecological Research Institute (PGOSERI), but 50 of them died and only 10 fish survived. They divided in two groups and cultured in separated concrete tanks. Fished were fed with trush fish twice a day at a feeding rate of 4-5 percent of body weight. Culturing period last about 6 months. All the fishes gradualy died through the culturing period. Average of specific growth rate in group 1 and 2 were about 0/49 and 0/77 respectively. The weight gain for group 1 (WG) was %75 for 75 days of calturing time and % 142 for 190 days of culturing time while for group 2 it was %31 for 54 days of culturing time and % 75 for 190 days . Average weight of fishes belong to group 1 reached from 950(g) to 2150 (g) during 6 months, while group 2 reached from 872(g) to 1271/5 (g) in 3 months. Feed conversion rate (FCR) for group 1 and 2 were respectively about 6.9 and 6.8. With regard to cost of trash fish (about 2000 Rials per kilogram trash fish) the per unit feed cost of cobia production were 13613 and 13730 Iranian Rials per kilogram, respectively for group 1 and group 2. Results revealed that obtaining live individuals of cobia is very difficult and also cobia didn t grow well in concrete tanks but if we have better coopreration of local fishermen and also provide some Equipment such as cages in order to rear cobia in the sea , it is possible to achive more succes and obtaining better results .

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The "fisheries and aquaculture industry innovation system" is one of the subsystems of Agricultural innovation system (AIS), also has many characteristics of that system .Since this section contains most of the components and AIS institutions, it can be examined as a part of the national innovation system. Due to the structure of the innovation system in the fishery system, a set of functions and institutions of this system are most important .It is therefore important that the system be analyzed and by reviewing the existing state institutions and functions of this system, the main problems and challenges identified. Understanding of the institutions and functions of fishery innovation system and analyze its problems and provide an improved model for fishery innovation system, is most activity in shaping major policy innovation in fishery sector. This step is very important to conduct innovative activities in the fishery sector. In this study, using a system perspective, the current status of the various factors affecting fishery development was assessed and the main drivers and challenges and solutions to improve the detection and analysis was provided. The results show that during the past eighty years, a number of research centers and educational institutions in the public sector have been developed .but this system, despite having considerable institutional capacity, is still in the middle stages of institutional development. In these years, the policy approach was linear, not system approach, so little attention has been to the development of science and technology intermediary institutions, coordinator organizations, venture capital funds, and the institutions of technology transfer ,particularly in the public sector.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Aquaculture of sturgeon species and their hybrids is being considered as an important substitution for sturgeon catch due to highly decrease of natural populations, artificial propagation and fingerling release in the sea. In this study, big bester, a new hybrid sturgeon (female beluga × male bester) was produced for the first time in IRAN. Sperm of 7350 ± 1682 kg male bester was used to fertilize the eggs of one 54 kg female Huso huso. The fries of big bester and control trestment of beluga were fed by artificial concentrated food (48-50% protein and 15-17% fat) after egg yolk absorbance, a period of feeding on Artemia and Daphnia. Results showed that rearing and feeding of bester broods was efficient to reach the fish to maturation stage and there is an opportunity to collect qualified ova and sperm from F1 generation. Meanwhile sex determination and maturity assessment of gonads were successfully done via laparoscopy method. The comparison of produced big bester fingerlings with control beluga fingerling showed that the weigth of big bester fingerlings has not significant difference with beluga's (p〈 0.05) at the age up to 2 months.At 3 months of age, beluga fingerlings showed higher weight (p〉0.05), but there are faster growth rate in big bester fingerlings from 3 months of age up to 5 months (p〉 0.05) in comparison with belugs fingerlings. Meanwhile no statistically significant difference was found between length of big bester and beluga fingerlings among any age. The results of current study showed the potential of rearing male bester to produce matured broods and collection of their sperm for big bester production.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Survey method has been performed using questionnaire forms for study of fish consumption per capita per year in Markazi Province due to lack of enough data on this field in the said province. Each questionnaire was completed by asking questions from each of the families as a statistical population sample either in rural or urban areas. According to the national census in year 2008 by Iranian Statistical Center, there are 364564 families living in Markazi Province out of which 207802 (57%) families are urbanites and 156762 (43%) are ruralist. A sample with total population of 2525 families were chosen with 1455 families living in urban and 1075 families in rural areas. Systematic random sampling was adopted in both areas which show people consuming fish once in 25 days with 5.8 Kg as per capita and per year for the whole Markazi Province.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Growth experiments were conducted in a random statistical design to determine the dietary requirements of Persian sturgeon, Acipenser persicus. In this study the effects of various dietary protein and energy and their optimum P/E proportion in diets was estimated on growth and carcass composition in A. persicus in the fingerling and grow out stage in three different phases. In the first phase, 16 semi pure diets with 4 protein levels (35, 40, 45 and 50%), each with four energy levels (18.5, 19.8, 21.1 and 22.4 megjoules/kg diet) and P/E proportion of 15.63 to 26.4 mg protein KJ-1 were formulated and fed to 960 A. persicus fingerlings, with a mean weight of 10.26±0.11 for 112 days. Three replicates were used for each experiment group. In the second phase, 432 A. persicus juveniles with a mean weight of 112.25±1.187 g were fed 12 semi purified diets with 3 protein levels (40, 45 and 50%) each with 4 energy levels (18.5, 19.8, 21.1 and 22.4 megjoules/kg diet) and P/E proportion of 18.9 to 26.4 mg protein KJ-1 for a period of 101 days using three replicates for each treatment. In the third phase, 384 juvenile A. persicus with a mean weight of 803.26±3.5 g were fed 16 diets for a period of 140 days. Two replicates were used for each treatment group. At the end of the experiment 30% of the fish population was randomly selected for carcass analysis to determine protein, lipid, moisture and ash content. Hepatosomatic index of the fish specimens was also determined. In the fingerling stage the fish fed 45 and 50% protein with 22.4 megjoules/kg diet energy exhibited the best growth indices [final body weight, percentage body weight increase (BWI%) and specific growth rate (SGR)] (P〈0.05). A diet containing 40% protein and 22.4 megjoules/kg diet energy with P/E ratio of 17.86 mg protein KJ-1 is the recommended diet for A. persicus in this stage. In the first phase higher growth rates were observed in fish fed diets containing 40% protein as compared to those fed diets containing 45 and 50% protein. Increasing energy levels to 21.1 and 22.4 megjoules/kg diet significantly increased (P〈0.05) growth indices. Highest values for growth indices belonged to fish fed diets containing 40% protein, 22.4 megjoules/kg diet energy and P/E ratios of 17.86 mg protein KJ-1. Final weight, BWI% and SGR in fish fed diets containing 40% protein were higher as compared to fish fed diets containing 35, 45 and 50% protein. Increasing energy levels to 22.4 megjoules/kg diet resulted in significant increase in growth indices as well as P/E ratios. Highest growth indices were recorded with diets containing 40% protein, 22.4 megjoules/kg diet energy and a P/E ratio of 17.86 mg protein KJ-1. Results obtained indicate that A. persicus showed better growth rates after acclimation to formulated diets, and therefore can be considered a suitable species for culture in captivity. On the basis of growth rate and carcass composition diet containing 40% protein, 20.1 to 25.9% lipid with a P/E ratio of 17.86 mg protein KJ-1 prepared from good quality fishmeal, fish oil and vegetable oil (sunflower or soybean oil) is recommended for feeding A. persicus in the fingerling and grow out stages.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Aquaculture activity is restricted mainly to rainbow trout in cold waters and carps in warm waters in inland waters of Iran as well as Fars Province. Probably for this reason saline waters of south and southeast Fars have not been used for aquaculture. These rivers are ecosystems with a diverse biota, it is predictable that can be found some there endemic aquatic organisms with a potential for aquaculture. Finding a suitable organism can help increase wise use of this natural resource, and local production and recruitment. However, it is highly needed to investigate various environmental elements prior to any use to obtain an estimation of the environmental consequences, parallel to finding usage potentials. In This study, we investigated Dehram saline river in south of Fars Province. Hydrological and physiographical properties, chemical composition of the river, and aquatic populations were sampled and measured monthly in 3 stations in one year. Salinity, electrical conductivity, and total dissolved solids increased from station 1 to 3, reaching from 6.6 g/L, 14.3 g/L, and 12800 µs/cm to 13.5 g/L, 17.7 g/L, and 16500 µs/cm, respectively. Dissolved oxygen and oxygen saturation were higher in station 3, and in autumn and winter. Nitrite showed lowest values in winter (0.006 mg/L) and highest values in spring (0.013 mg/L). Nitrate showed the lowest concentration in station 3 (0.43 mg/L), but no differences were seen among seasons. Ammonium showed no differences among stations or seasons. Phosphate levels in station 1 and 3 were 0.13 mg/L‌to 0.17 mg/L, with the highest level (0.29 mg/L) seen in the spring. Five species of fishes were identified in the river, which all were widely favored by local people. Some of these have been categorized as edible in previous studies, others as ornamental. On the other hand, intensive growth of an algal species throughout the river demonstrates a potential for algal cultivation. Significant role of algae in production of food for both man and livestock is now well recognized in aquaculture industry.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Trace elements incorporated into growing surface of the fish otoliths. It reflects the physical and chemical characteristics of the ambient water. Since otoliths grow continuously without re-absorption throughout the life of the fish, fish population growing up in different elemental composition. Trace element concentration in fish otoliths was analyzed by proton induced X-ray emission (PIXE). Otoliths were collected from Rutilus rutilus, during 1999-2000 in the eastern part of Caspian Sea. Ca, K, Sri Cu, Zn and Br in the otothis can be detected by using PIXE our results inform K, Sr, Fe, Zn as marker of this study. Therefore, in the two different conditions, separate 'age classes and without separate age classes, Astara and Turkmen regions were discriminated. Comparison of mean age in the different area show, deference of mean age is significant in the Astara and Turkmen.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The objective of this investigation was molecular study of Penaeus indicus population from Jask area. Samples were collected using bottem trawl net from three regions, East, West and Central Jask. DNA were extracted using phenol - chloroform method from muscular tissue. To investigate population differences of P. indicus, the specific primers were designed by sequence of Cytochrom oxidase I (CO I) gen in mtDNA molecule. PCR was performed using two spesific primers and the reasonable result achieved (600bp) for all samples. The PCR products of samples were digested by restriction endonuclease enzymes such as (Alu I, and followed by polyacrilamid gel Taq I, Hinf I, Rsa I, Dde I, Hinc II, Hind III, Hpa II, Ecor I, Pvu II) Observed by silver nitrate staining method. electrophoresis (PAGE) The bands of DNA were the same in all samples and the results indicated no polymorphism between samples. These results may suggested that cytochrom oxidase I gen in Penaeus indicus is a conserved gen or numbers of restriction endonuclease enzymes enough for the study of genetic variations in or all samples are belong to a single genetic population and was no possible for isolation of different genetic populations in this specie. Maybe the East, West and Central Jask are not sofar from eachother and absence of any Human made or different Biology in these sites are expected and no changes in genetic level.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Hamoun fish, Schizothorax zarudnyi, is an indigenous species of the eastern waters of Iran, which is exclusively found in this region. However recently drought occurrence in the Chahnimeh reservoirs (a semi natural water body) making them vulnerable to extinction. As an appropriate action to address this problem and according to the 3 side contract between Italian Government – UNDP - Islamic republic of Iran Government and the Italian government financial support to reduce poverty in the Province of Sistan-Baluchestan through the reinforcement and dissemination of aquaculture activities the project was developed at the Hamoun Research Unit by the Chahbahar Fisheries Research Center to record the breeding normative of 1 g weight larvae for restocking and other researching purposes. 331 broodstocks of the indigenous species Shizothorax zarudnyi weighing 800-2450 g were collected from the Chahnimeh reservoirs in early autumn, 2006. From 5/3/2007 ( the project is supported and communicated on 2010) , Ovulation was stimulated with three stimulators; pituitary extract (3-6 mg kg-1 body weight), GnRH-A (20-30 mg kg-1 body weight) and anti dopamine (10-15 mg kg-1 body weight) that was given in 2-3 doses to breeders. Of 169 injected breeders , some were injected On mid March of 2007 (12-13 ºC water tempreture) responded to the injection 25% ,while the rest were injected On April of 2008 (14-16 ºC water tempreture) responded to the injection 65%. In the present project of 167 breeders 82 were female and 87 male. Totally 30 female breeders released their eggs in different stages. 20 female breeders released their eggs completely, 3 breeders released half of their eggs and 7 released 1/3 of their eggs. The male breeders just injected in the final dose of hormontrapy and all were ready for releasing sperms however the ovulation in female breeders occurs between 353-428 h ºC and after the final dose of injection. Ripe eggs were stripped from the females and fertilization was done by the dry method. Fertilized eggs were transferred to veis incubators and troughs. Incubation period for eggs differs and larvae hatch out after about 910 days at an average water temperature of 12.5 °C. Maintained at 13-14 °C, complete absorption of yolk sac in Shizothorax zarudnyi larvae occurred after 5-8 days. Larvae were fed with a mixture of powdered milk and egg yolk in this stage followed by decapsulated Artemia cysts and nauplii of Artemia and then on formulated starter diets used for carps . Because the ponds were not ready, larvae were maintained in troughs for about ten days before they were transferred to two 1200 m2 earthen ponds where they reached a body weight of about 1 g. They were then handed over to the Iranian Fisheries department in the region. Larvae were fed with the starter feed SFCO in the earthen ponds. About 350 thousand larvae were stocked in two earthen ponds. Based on the results of present study and other studies we may conclude that artificial breeding in Schizothorax can be successfully achieved at 14-16 ºC in flow through systems using hormone therapy (combination of GnRha and anti dopamine) and larvae could be easily cultured in earthen ponds. However this species exhibits lower growth rates as compared to carps its high expenses could have an important role in economical feasible.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In present research work, variation and distribution of Sturgeon fishing lower depth of 10 meter of Caspian Sea were investigated. The CPUE and the population structure, such as total length, fork length and weight during 2003-2005 in southern part of Caspian sea were recorded. In 2003-2004, the total number of 300 fish were sampled including Acipenser Persicus 244 A.stelatus 35, A. nudiventris 120, A.goulden stadti, 9 . In the period of the next year (2004-2005) 412 Fish were lollected, consist of A. persicus 370, A.stelatus 14, A. nudiventris 8, A. goulden stadti 19, and one Huso huso. The CPUE (in night) in 2003-2004 For A.persicus, A.stelatus, A. nudiventris, A. gouldenstadti were 4/05 , 0/58, 0/20 and 0/15 respectively, where as it was 6.17 for A. persicus 0.23, A.s 0.13, A.n 0.13, A.g and 0.02 for Huso huso. The mean total length For 2003-2004 of A. persicus was 26 ± 10.86 cm A.s 49.7± 14.5, A.n 38±13.79 A.g 43.1± 10.25cm. In 2004-2005 it was 21/0±18.56, 52.5±25.53, 30 ±13.09 , 23.37 ± 12.2 and 50cm for A.p, A.s, A.n, A.g, Huso huso respectively.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The alarming rate of population growth has increased the demand for food production in third-world countries leading to a yawning gap in demand and supply. This has led to an increase in the number of hungry and chronically malnourished people. This situation has created a demand for the formulation of innovative and alternative proteinaceous food sources. Single cell protein production is a major step in this direction. SCP is the protein extracted from cultivated microbial biomass. Algae, fungi and bacteria are the chief sources of microbial protein that can be utilized as SCP. Produced proteins from these microbes have various nutrition values. SCP is the manufacture of cell mass using microorganisms by culturing on available agriculture, industrial wastes and fisheries by products. Fish wastes due to high protein are the most important substrates for SCP production. In this study, SCP production was done from Silver carp and tuna fish wastes (head, tail and vise versa) and cooked water of canned tuna factories. The used microbes were six genus and species of yeasts include Candida utilis, Saccharomyces cereviceae, Rhodotorula, Khyveromyces marxians, Zygosaccharomyces rouxii and Bacillus subtilis and B.licheniformis. The examination was done in bench scale and CSTR bioreactor (Continuous Stirred Tank Reactor). The effects of various parameters such as pH, temperature, time, supplemented substrates, method of inoculation of microbes, rpm were evaluated. Changes of microbial growth and protein contents were tested by using Optical Density (OD) and Makrokjeldal methods respectively. In end of examination, produced protein were extracted and lyophilized. The results showed that protein percentage in bacterial protein was than yeast protein but wet percentage in bacterial protein was low. Production value produced from tuna fish wastes was higher than (30-45 g/l) to Silver carp wastes (25-29 g/l) and cooked water (10-15 g/l). By adding supplemented substrates, production value has been increased. Candida utilis, in comparison other yeasts, has high activation. B.licheniformis has also had more activation than Bacillus subtilis. The results of the effect some parameters on fermentation showed that yeasts and Bacillus in pH= 5.4 and 32oC and pH=6.9 and 35oC were better than growth pH=6 and 25oC and pH=6.5 and 30oC respectively. Time of fermentation in batch and bioreactor was 54 and 21 hours respectively. High rpm has been caused increasing of microbial growth in bioreactor. The conclusion showed that with optimizing of the growth condition such as some parameters (pH, temperature, substrates and so on) produced SCP with high efficiency. However, produced SCP should be exanimated with other specific tests such as amino acid and fatty acid profiles, minerals, nucleic acids and so on. After full examination, this SCP as probiotic could be used in fish and poultry feed.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: At the present study, the water quality parameters monthly, environmental pollutants (pesticides, heavy metals and surfactants) seasonally, macrobenthos and bacteria (monthly) were done 2009 t0 2010 that located in Mangol Dam with longitude and latitude 52° 22' 49" N and 43° 15' 43" E, respectively. All water samples were collected from under-groundwater, surface water and wastewater in 12 stations and the results are as follow: The mean concentrations of DO, BOD5, COD, HCO3-, CO32-, TA, Ca2+, Mg2+, TH, TSS, TDS, Cl-, PO43-, NH4+, NO2- and NO3- were determined about 11.86±0.49, 5.95±0.11, 4.96±0.59, 28.1±6.1, 14.34±1.64, 36.30±2.33, 64.66±2.34, 28.84±1.52, 271.62±8.41, 0.37±0.03, 0.45±0.02, 28.02±1.96, 0.06±0.01, 0.010±0.001, 0.01±0.001 and 0.96±0.03 mg/l, respectively and also temperature, pH and EC were 12.7±0.61°C, 8.00±0.02 unit, 0.57±0.02 ms/cm in all samples. The surface water quality of Haraz River and its tributaries were suitable for usual applications such as aquatic matters, but based on BOD5 need to more attention for human usages. The water quality index (WQI) of surface water was classified in group II at all stations that shows serious water quality changes due to domestic and agriculture sewage. The groundwater was affected by different matters that caused change of water quality parameters. The wastewater was classified from good to moderate based on BOD5. The quality of wastewater was declined at down-stream with decreasing DO and pH and increasing BOD5 pollutants. The mean concentrations of aldrin and endrin were about 0.66 ± 0.59 and 0.71 ± 1.07 µg/l, respectively. The maximum seasonal concentration of organochlorin pesticides (OCPs) were detected in fall, winter and spring for endosulfan sulfate, γ-BHC, heptachlor, endrin aldehyde compounds about 2.85, 0.34, 0.14 and 0.14µg/l, respectively. The maximum seasonal percentage range of OCPs in fall, winter and spring for endosulfan sulfate, DDD and β-BHC were 42, 25 and 25 %, respectively. The maximum seasonal concentrations during fall, winter and spring were detected in Sorkhroud and Polechelave stations for only Fe about 1.48, 3.3 and 8.22 µg/l, respectively. Also for Hg in spring was determined about 17.2 mg/l. The mean concentration of Zn, Fe and Hg in water sampled were 0.23 ± 1.01 and 1.21 ± 2.00 µg/l, respectively and 4.65± 6.38 mg/l. The maximum concentration of linear alkyl benzene sulfonate (LAS) in summer was 971µg/l. The mean concentration of surfactant during fall, winter, spring and summer was 418.62 ± 52.16 µg/l. The maximum seasonal percentage ranges of surfactant during fall, winter, spring and summer in all stations of sampling were 76, 76, 100 and 100 %, respectively. Tottaly 15 families of macrobenthos were identified in Haraz River. The maximum mean of abundance and biomass in Sorkhroud and Nour-roud stations were 1177±256 ind/m2 and 3609 ± 935mg/m2, respectively. During Seasons, the maximum mean abundance and biomass were detected in fall and winter with 1185±444 ind/m2 and 3891±849 mg/m2, respectively. Water quality were classified by Hilsenhoff Biotic Index such as Sorkhrood station as "Very Poor", Isar town station as "Fairly Poor" and Karesang and Nor-roud stations as "good" qualification. The maximum of total coli forms and fecal coli forms in surface water were observed in Sorkhrood about 4.9 and 2.3CFU/100ml, respectively. The maximum of total coli forms and fecal coli forms in groundwater were observed in Halomsar and Absak stations about 4 and 1.7CFU/100ml, respectively. Maximum of total coli forms and fecal coli forms in wastewater were observed in Nor-roud station about 3.1 and 1.6CFU/100ml, respectively. The Ovary of nematode in wastewater were observed in Nor-roud and Isar Town stations about 30 through 124, respectively.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Inland water aquaculture activity have been known as an important approach for protein production while reservoir lakes recognized to have a significant potential for this purpose. Zanjan province in Iran with several dam reservoirs is suitable for aquaculture development. This study was conducted on biotic and abiotic factors of two lakes Shovir and Mirzakhanlo in order to determine aquaculture potentially of reservoirs. This two lakes located on different climatic region; Shovir located in region of semi arid area with very cold weather in winter, while Mirzakhanlo is in semi humid area and warm weather characteristic. In this study the plankton, benthos and fishes were assessed and the 15 hydrochemical factors were measured in order to determine the fisheries potential for fish introduction or release. The results showed that the phytoplankton abundance of Mirzakhanlo varied between 1.6 to 45.2 million cell/l. with 32 identified genus. The zooplankton abundance varied between 48 to 632 n/l. and 22 identified genus. The abundance plankton in shovir varied between 1.350 to 34.2 million cell/l. and 240 to 4500 n/l. for phyto and zooplankton respectively. Macrobenthos biomass were 21.4 and 0.34 g/m2 in Shovir and Mirzakhanlo lakes respectively while Chironomidae and Tubificidae families were dominant groups. The lake sediment organic matter were 4.3 and 3.2 % respectively. In ichtiology survey identified 5 and 7 species in Mirzakhanlo and Shovir lakes respectively. The hydro-chemical results indicated no restriction for aquaculture activity and according to trophic model both lakes are recognized as meso-eutrophic and eutrophic. Despite of climatic restrictions, the potential for fish production was estimated about 321 and 151 kg/ha for Shovir and Mirzakhanlo lakes respectively that can be improved by using agricultural and the other native facilities.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Artificial reefs are manmade materials deployed under water in order to improve invironment and increase the exploaitation of fishing area. Usage of artificial technic has developed due to increase of world pupolation and need to supply of protein, aim to restoring of natural spicialy rehabilitation of demersal fishes. It has effecte to increase the production in order sustainable exploitation. Coasts and Islands have destruction due to over harvesting from ecosystems and other activities by humans and natural, These caused many aquatic as demersal fishes has endangered in Persian Gulf and Oman Sea. The artificial reef is one way or method that can improve the environment and restore the aquatic. Iranian fisheries has established an artificial reefs area in west of Kish Island. This area has studied during one year, Data of assembeled fishes and physical sampling were collected in seasonal during spring, summer, autumn and winterd. Ther were tow tritment for sampling as artificial reefs site and a control site. Collected data has analyzed and evaluated by SPSS and Exel. The results showed that there was significant difference between the sites and assembled fishes in artificial reefs were more than control site. Consequently the artificial reefs can be a tool and technic to improve the marine environment and increase the production of fishes, especially the demersal fishes.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study focuses on molecular investigation of two commercial shrimp species of penaeus family namely as : P. merguiensis and P. indicus in order to find and introduce the genetic differentiations and also probable genotypes for monitoring and managing the genetic resources of populations in three major catch areas in the Persian Gulf and the Oman Sea. Only five out of the eight primers for p. merguiensis and four out of the eight primers for P. indicus produced good amplified PCR products with fixed annealing temperature. The rest of the primers were either not easily amplified or produced nonspecific bands. Seven and six alleles were found to be unique to each of the three populations in P.merguiensis and and two populations of P. indicus respectively. Occurrences of heterozygosity deficiency were found at most loci. These heterozygosity deficiencies in observed heterozygosity in compare to expected heterozygosity may be due to inbreeding, genetic drift and consequences of illegal overharvesting of P. merguiensis and P. indicus in the studied areas as well. Deviation from HWE in both studied species was significant in most microsatellite loci (P 〈0.001). We observed deviation from HWE in most loci with hetrozygosity deficits. The genetic variation results showed that the pairwise Fst values were significant between populations in both species. The assignment test for P. merguiensis revealed high gene flow between Hormoz and Jask and restricted genetic flow between Guatr and Hormoz populations .We observed high gene flow between Hormoz and Jask populations for P. indicus. It seems that the changes in immigration patterns of populations between Hormoz, Jask and Guatr areas in both species are depend on the influence of Persian Gulf currents or the life cycle of studied species. Alternatively, the presence of ecological barriers such as mangrove forests may result in restricted genetic flow between Guatr and both Hormoz and Jask populations.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This survey was designed in the Helleh region, to understand effects of aquaculture industry on coastal waters of Busher, in 2000. Sediment and water samples were collected from effluent canal, influent canal and sea during the 7 month period (harvest and post harvest season). The variations of selected water quality parameters were controlled monthly. Results show that, in effluent canal, estimated parameters of water was higher than other stations every month, and tend to decreased after being discharged into the sea and got closed to normal level. Comparison of above data with permit able range of municipal and aquaculture waste and comparison of present data with data of normal condition of region, show that, aquaculture industry of Helleh region has not had any obvious negative effect on the coastal waters of Bushehr, in the culture year of 2000.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The culture of Iranian Shrimps began in Bushehr in 1372. Early the green or the pink tiger shrimp (Penaeus semisulcatus) which were found in most habitats of the Persian Gulf and Oman Sea were considered in breeding and reproduction, but due to reproduction difficulties, it was rather replaced by the Monodun imported species, then the production of the white shrimp Metapenaeus affinis and Then P.merguensis in some southern areas of the country such as Hormozgan has began in small scale,but none of these species couldn't meet the economical needs of the consumer society and they were not indelible and dominant enough in the shrimp aquaculture industry of the country until breeding of Indian White Shrimp (Fenneropenaeus indicus) has began in large scale in the provinces of Khuzestan and Bushehr, which are seen as the heart of shrimps reproduction and breeding, and in Hormozgan and Sistan. the incidence of white spot viral syndrome (White Spot Syndrom Virus) led to the import of the western white leg shrimp from the USA (P.vannameii) by the Iranian Fisheries Research Organization (IFRO) in 1383, at the present all southern and northern breeding farms of the country (fundamental measures has been carried out in line of shrimp reproduction in Gorgan province in 1386) has put the breeding of this shrimp species as the single breeding species at top of their agenda. The subject of feeding the breeding shrimps is widespread and regarding to the non-proprietary species there are still some species which contain high protein than the other species in studying the different shrimp species requirements. In Iran due to the scarce factories producing the shrimp's food, the alimentary compound of the breeding shrimps during industrializing years of this type of breeding even after the non-aboriginal western white leg shrimp species being inclusive was stable and through different biological processes including: Naplies which feeds from its yalk and by entry into zoa stage start eating only small-sized phytoplankton. And by entry into the stage between zoa and mysis it feeds from phyto and zooplankton simultaneously and entering the post-larva stage it stars sarcophagi. and after 15 post-larva stage entering the growth and transition stage to earthen ponds feeding by concentrated industrial foods in large and small packages depending on young and adult mouth and due to feeding requirements of every stage the alimentary compound is nearly as follows: Digestible protein, energy, fat, carbohydrates, vitamins and minerals will start and the final product will be released in market Or they will be used in the later years of laying eggs and feeding for brood stock. In feeding section the details of every stage and their food's nutritional needs at each stage and will be fully described.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was performed as a research program in Agricultural Research center of Kerman since 2000. During one year identification of fish in qanat basins of Markazi desert and Sirjan desert was carried out. In this study Electroshoker and salik net with o.5 and 1 cm network and padel net with 1cm were used to catch the fishes from 73 series of qanat. Parameters estimated in qanats (pH, Weather Temperature, Oxygen , Ec,...) Specified in sum 767 from fishes which were in 2 order 3 family and 3 species as: 1-Order: Cypriniformes. Family: Cyprinidaea Species: Capoeta damascina (Valenciennes, 1849) family: Balitoridaeb species: Schistura sargadensis (Nikolskii, 1900) 2-Order: Cyprinodontiforms Family: Poecilidaea Species: Gambusia affinis (Baird and Girard, 1853)

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Accurate scientific and practical plan for achieving the goals of the Islamic Republic of Iran within the framework of Vision development 1404 , is the infrastructure achieving sustainable development of the country . Order to achieve the above mentioned objectives and in order to the comprehensive development plans in the country, Iranian fisheries research organization adjust the fifth developing plan for support of executive related departments in country with mobilization a large number researchers consists of several working groups of ifro affiliated research centers . The fifth developing plan consist of three chapters for report of the forth developing plan and intrudction of research , construction plans and financial support (budjet) for period of 2011-2014 A.C .

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In order to introduce a new sturgeon hybrid to aquaculture industry, the possibility of hybrid production by crossing between Siberian and Persian sturgeons and its comparison with its pure parents using morphometric, molecular, cytogenetic and growth performance were conducted. For this purposes eggs and sperms of two pairs of Persian and Siberian sturgeon were collected and reciprocal crosses with 4 treatments were conducted. In treatment 1, Persian sturgeon as control (male and female of Persian sturgeon), treatment 2 (as type I hybrid) from crosses of male Persian sturgeon with eggs of female Siberian sturgeon in treatment 3 (type II hybrid) using crosses between male Siberian sturgeon and female Persian sturgeon and finally treatment 4 by crossing male and female of Siberian sturgeon were used. For each treatment 200 gr. of eggs were fertilized with one ml. of sperm and morphological, meristic parameters of parents and offspring were recorded. For growth comparison of fingerlings of control and hybrids in 3 different phases were measured. In phase (I), larvae rearing from 46 mg. up to 6600 mg. were conducted for a period of 10 weeks. Samples were collected once every two weeks and biological parameters were recorded. In rearing phase II for a period of 330 days, growth comparison were conducted for fish from 6.5 gr. to 750gr. and biological parameter were recorded once every month, where daily growth rate (gr./day), FCR, Specific growth coefficient (percentage per day), increase of body weight, food efficiency and protein efficiency ration were analyzed. In rearing phase (III), from 600 gr. to 2000 gr. for a period of 43 weeks, similar to phase (II) all biological parameters were analyzed, where 26 morphometric and meristic parameters of pure and hybrid parents and offspring were compared both for individual and ratio status of parameters. For genetic analysis and variation between control and hybrid specimens the microsatellite analysis were conducted. For this purposes, genomic DNA were extracted from fin tissues of brood stocks and fingerlings and 10 pairs of specific primers (Afug 56, Afug 63, Afug 241, Afug 229, OX 27, Afug 686, Afug 195, Afug 12, IS 68 and Afug 160) were used. Then PCR Products were electrophoresed on 6% polyacrylamide gel and then were stained with Nitrate silver and alleles were evaluated based on its length (base pair). Cytogenetic analysis and number of chromosome of control fish and hybrid were compared using white blood cell culture method and chromosome spread of parents as well as offspring were prepared by Gimsa staining and were visualized under light microscope. For sexual gonad development, laparoscopic method with light camera (Model M-CAM 1700) were used to differentiate 34 pieces of control and hybrid fish and gonad status of ovary and testis were analyzed. In present investigation the results indicate that, in phase (I) of larvae culture, a significant differences were observed after 10 weeks of feeding (p≤ 0.05) in hybrid and control groups. Maximum growth rate were observed in Siberian sturgeon larvae, followed by type I hybrid, type II hybrid and Persian sturgeon larvae. In phase (II) , growth rate of Persian sturgeon were lower than Siberian sturgeon. For period of 330 days of rearing periods, the control Siberian sturgeon had 1.4gr. weight increment per day, while Persian sturgeon had only1. gr./day. However the hybrid type (I) had an average daily growth of 1.5gr/day and lower growth performance were observed in type II hybrid with daily growth of 1.5 gr./day. In phase (III), growth rates were varied at different age group. The daily growth rate of Siberian sturgeon was 4.5gr/day, while is control Persian sturgeon was 2.6 gr/day. The Siberian sturgeon within 47 weeks of rearing grown from 655 gr. to 2016 gr., however Persian sturgeon for the same period reached from Siberian sturgeon with male’s Persian sturgeon had higher type II hybrid showed opposite results for the same growing period and reached from 640 gr. to 1680 gr. In conclusion of growth performance can be stated that the hybrid from crosses between female Siberian sturgeon with male Persian sturgeon presented highest growth rate in comparison to control hybrid. This excellent achievement can be considered as a big step toward sturgeon aquaculture for meat and Caviar production. In analysis of morphometric characters 26 parameters were investigated which in 18 parameters showed significant differences (p≤ 0.05) which indicate clear distinguish able of hybrid performance in compare with parents where Siberian sturgeon has longer snout compare to Persian sturgeon and its size are intermediate in hybrids. In relative ratio of characters, out of 9 characters totally 7 characters showed significant differences (p≤ 0.05). In molecular analysis using microsatellite primers totally 400 DNA samples of hybrid as well as the control fish were investigated. Totally 784 allele were detected in which 363 alleles were belong to control fish and 421 alleles in hybrid. In this study, 19 alleles with different sizes were observed in hybrids rang from (120bp) in Aox27 primers (400bp) in AFUG229 primers. In comparing of all primers, the primer AFUG160 and AFUG195 with 2 alleles and primer AFUG686 with 7 alleles showed the minimum and maximum allele numbers.The banding patterns were monomorphic in 66 loci of control fish and 65 loci of hybrids, and in 77 loci of control fish and 73 loci of hybrids were diploid in 9 loci of control fish and 12 loci of hybrid fish were tetraploid, in one locus of control samples and 3 hybrid samples were pentaploid and finally within 10 pairs of primers 7 sets were disomic, 3 primers (Afug241, Afug686, Aox27) were tetraploid and 6 primers of (Afug63, Aox27, Afug688, Afug12, Is68, Afug160) showed excellent allele heritability which indicate the high efficiency of microsatellite techniques. In evaluation of the status of gonad development as well as determination of sexual stages laprascopic method indicated that out of 9 samples of Persian sturgeon (4 females and 5 male) were in stage of 2 & 3 gonad development. Out of 8 Siberian sturgeon (4 males+4 females) stage 2, 3, 4 were detected. In 7 gonad samples of hybrid (Female Persian and Male Siberian) totally 5 male and 2 female were observed at stage 3 and 4 gonad development. In analysis of 10 samples of type II hybrid (female Siberian × male Persian) 3 female, 6 males and 1 deformetic were observed. Cytological studies on 30 metaphase palate from 6 fish samples showed that the number of chromosome in hybrid are as same as parents 2n=250±10 where all control and hybrids similar to its parents showed 240 chromosome. In conclusion, It can stated that, this study successfully introduced a new sturgeon hybrid for sturgeon farming both for meat and caviar production. Considering the common name of sturgeon parents used for production this hybrid, we call this hybrid as (Siper) where Si instant for Siberian sturgeon and Per comes from Persian sturgeon.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Population genetic structure of Vimba vimba persa was investigated using microsatellite markers from 4 regions along the Iranian coastline of Southern the Caspian Sea (Anzali lagoon & havigh River in Gilan province, BabolRoud River in Maz&eran province & GorganRoud River in Golestan province). Genomic DNA from 121 specimens was extracted from fin tissue by phenol-Chlorophorm method & PCR reaction was accomplished with 17 microsatellite primers, out of 17 microsatellite primers 14 loci were amplified, in which 10 of them were amplified with reasonable polymorphism & 4 were monomorphism. Totally 302 alleles were identified on average 7.5 Observed & expected heterozygosity averages were 0.80 & 0.77 respectively. Most cases significantly deviated from Hardy-Weinberg equilibrium (p 0.01). The estimation of Fst (p 0.01) revealed significant population structuring & estimate four population of Vimba vimba persa is identified in the Caspian Sea. These studies were to apply & develop population genetic approaches to assist conservation, sustainable harvest & restocking of these populations.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This survey carried out from 2012 to 2013 to study of suitable site selection for carp and trout fish species for responsible aquaculture possibility by data collection and literature study.During the survey ecological factors including physical and chemical factors,geological factors including topography and type of soil,biological factors including selection of species and control of invader animals,climatic factors,geographical factors and pollutant including heavy metals,agricultural pesticides Surfactants and crude oils were recognized.Also in comparing with standard levels suitable rate of each important factor in aquaculture were studied.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The Iranian sturgeon ( Acipenser persicus) is one of most commercially important sturgeon species in the Caspian Sea and it is considered as an endemic sturgeon of the south part of the Caspian sea. Most of its populations distributed around the south coast of the sea and also the most Iranian caviar production belonged to this species. Due to overfishing, degradation of the rivers conditions as the natural reproduction places the stocks of the fish is decreasing and that is why the Islamic republic of Iran releases millions of its fingerlings to the rivers that end to the sea to stock restoration. With regard to the high expenses of the artificial propagation and fingerlings production, finding and creation of better output will play an important role to decrease the production expenditures. Now days, the decrease in the natural stocks of broodstocks and also the low quality and immature caught broodstocks are the fundamental problems which the industry of sturgeon mass production faces. These not fully matured brooders do not response the hormonal induction processes at the sturgeon hatcheries (because they do not reach to final maturation and some of them having polarization index greater than 10 ) and large numbers of the caught and transported brooders to the hatcheries will not produce any fry ( as the data distributed and published by the sturgeon hatcheries about the production techniques). The main objective of the research study is to apply a new method for artificial reproduction (LHRH-A implantation) to gain the best output from the Iranian sturgeon brooders caught and transported from the Caspian Sea which will be done for the first time on sturgeons and there is not still any report of the application of this procedure ( LHRH-A implantation )over these kinds of fishes. Most published report on the method belonged to the teleosts.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In this study ichthyoplankton, phytoplankton, zooplankton, benthics and hydrochemistry parameters were studied. 9 out of 11 sampling stations were in the Gorgan Bay area and 2 of them were in the mouth of the Bay. Ichthyoplankton survey were carried out in 6 stations and the other surveys such as phytoplankton, etc were done in 11 stations in 2009. Gorgan Bay map and position of sampling sites are given at the end of material and methods section. Biomass and frequency of phytoplankton at different stations, months and seasons were calculated that are presented in Tables 1 to 3. Hydro-chemical parameters such as water temperature, air temperature, visibility, DO, BOD5, pH, Alkalinity (bicarbonate, carbonate and total) , Hardness (calcium, magnesium), CL,EC, Salinity, N (nitrate, nitrite, NH3), Phosphate were measured and results are shown Table 11 . Biomass (mgr/m3) and numbers (ind./m3) of 47 genus of different phylum of phytoplanktons such as Cyanophyta, Chlorophyta, Pyrophyta, Chrysophyta and Euglenaphyta as well as different phylum of zooplanktons consist of Protozoa, Arthropoda, Rotifera and Cyliophora were estimated. Frequency of phytoplanktons (ind/m3) was 131.2, 1.6, 65.3, 18.9 and 23.8 millions respectively. Furthermore, their biomass was 308.4, 1.9, 1358.9, 295.1 and 124.7 mg/m3, respectively. The maximum and minimum numbers were observed in Leptolingbaya belonged to Cyanophyta and Diatoms of Chrysophyta 84.3 and 0.025 million ( ind/ m3) respectively. The maximum and minimum biomass was observed in Gyrosigma (Chrysophyta) and Tetradron (Chloropyta), 12317.3 and 0.014 mg/m3 respectively. The average biomass of zooplankton was measured 531.74, 30.77, 225.07, and 96.26 mg/m3 respectively and the total was attained 883.83 mg/m3 Table 5 . The maximum and minimum biomass of all zooplanktons was estimated in November and July, 3446.23 and 6 mg/m3 respectively. Furthermore, the maximum and minimum numbers of zooplanktons were found in September and July which were 48.4 and 0.002 millions respectively and the average number was 8.4 ind/m3 Table 4 . Also numbers and biomass (gr/m2) of 11 families of benthos which inhabit Gorgan Bay such as Annelida, Mollusca and Arthropoda were investigated in separated stations and distinctive months. The maximum and minimum biomass of benthos was observed in January (42.91 gr/m2) and March (0.6428 gr/m2). The biomass was considerable in April, February, August and December (Tables: 4, 5 and 6).

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was conducted to codify the guideline and indexes of broodstock management in salmonid fish and cryopreservation, selective breeding and sex reversal in hatchery to attain responsible aquaculture development. Cryopreservation includes of sperm and egg in term of economic is important in aquaculture. In this report mentioned the type and name of material. All female and sex reversal fish are produced by steroid hormone which mentioned in this report. Selective breeding include individual selection and family selection which could be used for genetic improvement of fish.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Research project was conducted on some biological aspects of lobster in Oman Sea in the years1385-86. During the project some parameters such as length distribution, spawning peak, length (Carapace) at first maturity, the length at which 50% of the lobsters were found to be mature sexually, sex ratio, relative frequency index and population dynamics parameters were examined. Quantitative factors and catch composition of lobster including the amount of catch in terms of weight and number of ovigerous-female, under-sized specimens and so were compared by monthly. According to the results of present study caught specimens were male with carapace length of 22 and 110 mm respectively. The smallest ovigerous female was taken as 46mm CL when length at first maturity was estimated to be 69-70mm. the male: female ratio was 0.7: 1.3 for the whole year. A closed fishing season for the species is proposed during spring and September and October in Oman Sea. A large amount of the catches for the all sampling months came from the under-sized specimens, consist of 50 to 85% of the total catch. Therefore, presence of these small length groups have direct effects on time of closing season. Population dynamics parameters were calculated as follows: K=0.57 year, L∞= 103mm and Ǿ = 8.69. Total mortality (Z), natural mortality (M) and fishing mortality (F) coefficients were calculated as 2.73, 0.87 and 1.86, respectively. Exploitation ratio for the whole population was 0.681, indicating an overexploitation of the species. Based on the available data, the recruitment pattern for lobster was obtained for two season including spring (major) and autumn (minor). Catch per unite effort (CPUE) was calculated as 0.908 kg/Cage/day which was higher in comparison with two other areas including Ramin and Chabahar. Examination of CPUE for a 10-year period showed a decreased trend and the reduction for the year 1386 was about 30% when compared to the data from the year 1375. Moreover, the mean length during 1369-70 to 1385-86 (present study) decreased for the three areas including Pozm, Ramin and Chabahar, so that the carapas length decreased up to 10 mm in length. This sudden decrease in mean length during the 10-year period should be considered as a serious precariousness for sustainable exploitation of lobster in Oman Sea along Sistan and Baluchistan province.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was conducted to determine the physico-chemical characteristics of water during four season and 8 transects (Astara, Anzali, Sefidroud, Tonekabon, Nowhshar, Babolsar, Amirabad, Bandar Tourkman) in the Southern of Caspian Sea in 2009-2010. 480 samples were collected at different water layers and then physicochemical parameters were measured based on standard methods. Result of this study showed that surface water temperature was varied from 7.2 to 29.8 ◦C in winter and summer, respectively. Minimum fluctuation of water temperature was observed at 100 m depth (6.8-10.3 ◦C). Mean value of water transparency was obtained 4.91±0.24 m. This value increased from inshore to offshore. pH value was fluctuated from 7.15 to 8.83 unit with variance of 1.54. Maximum DO concentration was observed at surface layer (8.40±0.08 mg/l) with 137±18 saturation and minimum was at 100 m depth (6.46±0.18) with 86.1±2.8 saturation. The nitrite, nitrate and ammonium concentration were ranged 0.0-0.2, 0.0-4.6 and 0.05-7.12 µM, respectively. Maximum value of TN was observed at inshore and minimum at offshore (100m). Nitrite concentration decreased from inshore to offshore but increased from surface to the bottom (100 m). The inorganic phosphorous increased at surface water and also at the bottom. Trend of inorganic and TP was similar. Minimum of N/P ratio were observed at summer (5.48±0.38) and maximum value at winter (9.13±0.46). This value of N/P ratio showed that the growth of phytoplankton was limited by nitrogen. The dissolved silicate was decreased from spring (230.7±6.65 µg/l) to winter.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: An Environmental Impact Assessment was undertaken to identify existing values and characteristics of physical, biological and socioeconomic resources within the area proposed for the Aquaculture development in BibiShirvan Ab-bandan (small storage dam). The proponent (Fisheries Department of Golestan province) intends to redevelop fish culture in a constructed water body which is used mainly by local farmers for storing water to meet crops water needs especially at dry periods of the year. The area being examined as part of this environmental impacts assessment is located approximately 40 km west of Gonbad city,Golestan province in northeast of Iran. The site located at 54° 47'- 54° 52 ' (East longitudes) and '37 ° 10'- 37° 11' (North latitudes). The Bibi Shirvan Ab-bandan sealed with an area of about 580 acres, is a collection of three distinct basins that are connected with each other at several points. At present, there are some problems for water supply and maintaining full reservoir levels which cause the Bibi-Shirvan reservoir have always been empty partly and farmers could not been utilizing all its capacity. At the operation phase, the supply water will be provided by pumping through the left coast of Gorgan river via an irrigation concrete canal which is constructed by the Organization of Jihad-e-Agriculture and also, two small streams namely Ghareh-tekan and Bayram-shally. For better management of fish culture it is proposed a plan, which increase the volume of the Bibi- Shirvan reservoir by heightens the circumference embankment and construction of the interior retaining wall that will divide the reservoir into smaller pieces of ponds. It also proposed the installation of other pumping stations on abovementioned streams to supply water for combined requirements of cultivated crops and fish culture. The total surface area and storage capacity of Bibi Shirvan reservoir will be about 500 hectares and 8.1 million cubic meters respectively, after the construction of embankments. Environmental impact assessment for the project in terms of design features is mandatory and subject to article 2, paragraph (e) environmental impact assessment regulations. There are some problems for developing optimized fish culture practices and operations in the area. The unknown ownership of reservoir, absence of official right of water allocation, limiting capacity of reservoir reduced surface area for fish culture , lack of hydraulic system structures for water supply control, and drainage are among the problems that restrict fish culture practices and will not allow to attain optimum production. Therefore, the proposed plan including improved methods and rehabilitation of embankments was placed on the agenda of the Golestan Province, Department of Fisheries. For optimization studies and achievements to update information using Geographic Information System (GIS), the maps of the study area were provided. Thus, in first step, the digital map with a scale of 1:500 to 1:2000 AUTO CAD format that shows the project location, have been constructed. General location map of the region, residential areas and villages, roads and other maps needed in studies using these layers were produced. Next, the outputs for Cartography maps were prepared using ARC GIS software. The spatial layers were performed by using the GIS soft-wares and advised which suggested by different expert teams (groups of soil and water pollution studies, social environment, economic and cultural, biological environment, climate and hydrology). The output of digital spatial data, spatial analysis and cartography as various maps, tables or diagrams and graphical analysis were used in the reports. Since implementing this Project could be created various impacts in different environments (physical, biological, socioeconomic and cultural) The EIA study was undertaken .For facilitate decision making process on project implementation, the modified matrix method has been used. In conclusion, the results of EIA matrices shows the action of Project with environmental mitigation measures (+410) is superior to no action Project (-79). It is therefore considered that the Bibi-Shirvan Project will have a minimal impact on the environmental values of the area. The Project will provide favorable overall positive outcomes for indigenous peoples and fish farmers in the region. Also, problems such as excessive migration of rural people, lack of employment and consequently low level of income and welfare of the people will be improved. Therefore, the Project with consideration of environmental mitigation measures is recommended .

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Recently, we have been encountered to increasing jellyfish biomass in the world tropical coastal waters and due to their role in ecosystem food web, can be made direct and indirect effects on marine stocks and causing problems for fisherman’s communities. Now, the number of studies on this animal groups and factors which caused to jellyfish bloom has been increased. Present study was carried out for identification and density estimation of jellyfishes in the East and West coasts of Khure-Musa canal in Khuzestan coastal waters in North-West of Persian Gulf. Monthly samples were collected by two sampling methods from randomly sites in studied area in day light during February 2007 to January 2008. Small jellyfishes were collected by using 500 micron mesh net and density was calculated as number per 10 square meters. Large jellies were collected by using shrimp trawl net and quantity measurements were done by swept area methods. Totally, 18 species including 9 hydromedusae, 2 Scyphomedusae, 3 Siphonophorae and two Ctenophores were identified. Generally from 5357 separated small jellyfish, 56.6 % was from East and 43.4 % was from west coasts. Ctenophore, Pleurobranchia (31%) and then the Hydromedusa Eiren hexanemalis (22%) were the most abundant taxa. The maximum number of jellyfish (1080 per 10m2) was observed in western coasts in July and then decrease in summer and autumn. In contrast to Eastern coasts, the highest frequency (655 per 10 m2) was observed in western coasts in September. Two peaks of frequency in May-June and in summer to fall were observed in this area. The highest frequency of macro jellyfishes were in July and mostly in East coasts (2811 per km2). During February 2007 to February 2008 Catostylos tagi and Chrysoara hysocello were dominated. Monthly jellyfish abundance variation showed inverse relation between Eastern and Western coasts. The maximum diversity was in August in Eastern coast and July in Western coasts. The minimum values were calculated in winter months in two areas. Obviously, with increasing jellyfish abundance, in summer months, fish larvae population decreased. Jellyfish explosion can be caused by several factors, fishing pressure and removing top predators from food web, climate change, global warming and eutrophication phenomena are defined as the major factors in jellyfish bloom in worldwide tropical coastal waters. Therefore human activities enhance the occurrence of this phenomenon. Recent drought, decreasing freshwater input, increasing waste discharge and overfishing are probable causes for jelly blooms in Khuzestan coastal waters.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Present study was conducted in shrimp farm located in east of Chabahar in Sistan and Balouchestan province from August 2001 until May 2003. Fourteen stations at three locations (i.e. supply channel, drainage channel and Gwatr Gulf) were selected to determine physical and chemical factors such as; temperature, salinity, dissolved oxygen, pH, nitrate, nitrite, ammonia, phosphate, silicate, total suspended solid and turbidity. Sampling was conducted twice a month during the shrimp culturing period and monthly at other times. Water samples were collected every two weeks to determine Chemical and Physical factors, Chlorophyll a, Phytoplankton and Zooplankton. Sediment samples were collected seasonally to examine Macrobenthos, Grain size and Total Organic Matter. The results revealed different bioenvironmental area in the three locations (i.e. supply channel, drainage channel and Gwatr gulf) over the study period. One-way analyze of variance of chemical and physical factors indicated significant difference between different times as well as different stations (p〈0.05). The negative relationship was observed between the culturing production area and the amount of average dissolved oxygen, pH and total suspend solid. An increase in the culturing production area in 2002 as compared to that of 2001 causes a decrease in the amount of these factors. However, it dose indicates positive relationship with nitrate, nitrite, ammonia, transparency and chlorophyll. Forty nine phytoplankton genus were identified, which mainly belong to diatoma, dinoflagellate and green blue algae. Diatom was dominated among these groups. Diatom and green-blue algae and diatom comprised %79, %69 and %64 of phytoplankton flora respectively in supply channel, Gwater Gulf and drainage channel. Copepod and thintinnida were the major groups of zooplankton. High abundance of bivalve, Polychaetes and amphipoda were found in supply channel, Gwatr Gulf and drainage channel respectively. Analysis of grain size showed the existence of sandy sediment in Gulf of Gwatr and sandy-loom in supply channel. Drainage channel sediment found to be sandy-loom, sandy-silty-loom and sand loom. Among three locations, the highest TOM was found in drainage channel. In last location, production area in 2002 increased as compared to that of 2001, which showed negative relationship with salinity, nitrate, nitrit, ammonia and chlorophyll a. Drainage of the Gwatr s shrimp farm did not cause any thermal contamination in the location. Shrimp aquaculture activities caused an increase in the average of salinity, range of pH in the Gwatr Gulf.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The study on economic aspects of tilapia culture in Iran is a subproject of a comprehensive project on introduction of tilapia to central regions of the country. Two types of red and black of the exotic tilapia were imported from Indonesia in 2009 for research and were transferred to the research center of Bafgh. Since tilapia has capabilities in aquaculture such as resistance to diseases, fast growth, tolerance to high intensity, growth in freshwater, brackish water and in saline water, also ability to grow in pond, cage and aquaculture tanks, so it is likely that it’s production be of economic advantages. Therefore the present study looked at economic aspects of raising tilapia. The factors examined were feed expenditures, wages, fertilizer spending, costs of fish fingerlings, and pond preparation in two treatments of black and red tilapia in 0.45 hectare earth ponds, 3m2 aquaculture tanks with two replicates. Results of data analyses showed that benefit of each pond for black tilapia would be about 5968763 Rials. For red tilapia this amounted to 5939696 Rials. On total the benefit for black tilapia pond culture was 11940444 Rials while this for red tilapia was 11879392 Rials, so we can conclude that pond culture of tilapia is beneficial. Culture cost of black tilapia in tanks with 57 Kg outcome, was 2540600 Rials. Accordingly the cost of each kilogram of fish is 44572 Rials, so with 20 percent benefit, the price for each kilogram is 53485 Rials. Also for producing 37 kilograms red tilapia in tank some 2531500 Rials was expended. Each kilogram red tilapia cost 68419 Rls to produce which is much more than other cultured fish and if we expect 20 percent benefit the price of each kilogram of red tilapia would be 82103 Rls. So it is concluded that tank fish culture of tilapia would not be beneficial and economically speaking there would be no point to raise tilapia this way.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Pearl aquaculture production is one of the most valuable industry in the world. Black lip pearl oyster Pinctada margaritifera is one of the most important commercial species in the world that it is used for breeding and production of pearl. To determine the basic requirements for black lip oyster growth and development of gonad under controlled conditions, Pearl oysters with a total length of 80 mm shells that were in stage 2 maturation of the island Hendorabi collected by diving operations and were transferred to Bandar Lengeh Research Station Persian Gulf mollusks. Pearl oysters with length of 95 mm shells, was passed were all male. 25 percent of them with a total length of 100-110 mm and all with a total length top of 110 mm were female. Then thay were fed for 45 days in appropriate conditions of temperature and photoperiod with March until late spring. Ggreatest physical growth related to Diet with micro-algae Isochrysis sp (T.ISO), Cheatoceros calcitrans, Cheatoceros mullerii, Pavlova lutheri and Tetraselmis suecica, which with the other treatments were significantly different (p〈0.05). Most gonadal growth of the group 4 (Isochrysis sp (T.ISO), Pavlova lutheri, Cheatoceros calcitrans, Cheatoceros mullerii ) was 70% in the treated females were spawning. Minimum total length of black lip oyster in habitat of 80 mm, which were collected from a depth of 2 meters. There is no relationship between total length Oysters and depth of 4 to 10 meters depth and minimum total length was 115 mm. The most density of Black lip oyster were observed in the depths of 4 to 7 meters in the northwestern part of the island Hendorabi. There were stronger correlation between biometric data, length-weight relative to length- length.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was done in 2008 on agricultural sewage for aquaculture in six stations. Two stations were in AhvazKhorramshahr road near sugar cane plantation sewage in west Karoon, Two stations were in Ahvaz-Abadan road in sugar cane plantation sewage in east Karoon and Azadegan sewage and two stations were in agricultural sewage in the north of Ahvaz. Monthly Samplings of planktons, physical, chemical parameters were taken during Aprile to March 2008. In addition seasonal sampling of benthic fauna and fish were carried out. Heavy metal and toxic samples were done only one time during the study period. Measurement of some parameters such as temperature and pH were done during sampling procedure and other parameters were measured in laboratory based on standard methods. Heavy metal concentrations were measured with polarography and voltammetry techniques and toxic substances were extracted and concentrated to determine the concentration of them in water using GC device. For identifying phytoplanktons, 1 liter water from middle depth was sampled in each station and 4% formalin was added. Zooplankton were sampled monthly using 55µ plankton net. Fish samplings were done using cast net regarding regional condition, and sampling from benthic invertebrate of river were done using Petersen grab with 15.5cm*15.5cm. Results of water quality index (WQI) from Koushk Talaieh (station1), Artificial lake station (station 2) , Farabi(station 3), and Azadegan aquaculture sewage station (station4) showed that water quality is in group four. WQS index was used for Maleh(station 5) and Pumping station ( 6 station) stations due to high levels of salinity. The WQS results showed that water quality of these stations were moderate to low. Heavy metals in water, the ordination is as follows: Ni 〉 Zn 〉 Pb 〉 Cu 〉 Co 〉 Cd 〉 Hg Mercury levels in all cases were less than the standard and all the elements have been somewhat less than chronic toxicity level. The values of all studied metals are lower than the WHO limit. The results indicated that chlorinated pesticides in all samples are at low level and they are at acceptable levels for aquaculture. In this study, 34 genus of phytoplankton from Bacilariophyceae (diatoms), Cyanophyceae, Chlorophyceae and Dinophyceae were present with 11, 8, 14 and one genus respectively and the percentage of frequencies were respectively 37.64, 34.26, 23.24 and 4.87%. In general phytoplanktons of wastewater were in modest condition for using in aquaculture. In this study, about 1,000 samples of zooplankton in 6 stations were identified and counted during the year. The high percentage of zooplankton were belonged to three groups of protozoa (mainly ciliates), Rotifer and copepods were. The Rotifers with more than 90 percent frequency had the maximum prevalence. The dominant rotifer species was Brachionus calciferous. Although in this study the dominant group of zooplanktons was rotifers but the frequency is not high. Therefore we cannot call this circumstance as eutrophic condition, however the growing trend of high food phenomenon has announced. High benthic diversity was observed in Artificial lake wastewater station and Azadegan aquaculture sewage station. The most frequent taxa were in Azadegan aquaculture sewage station during autumn and winter which belonged to resistant Chironemidae larvae. In Koushk Talaieh, Farabi and Maleh stations, Chironemidae was dominanted and in Pumping station Coleoptera was dominant group. Hilsenhoff index showed that most of waste water stations were in poor to very poor condition, indicating that they were heavily contaminated. The data analysis of macrobenthose data in wastewaters based on Satropi and biological value (Z) showed that potential of fish production in Azadegan and artificial lake was high during the year. These two water sources have suitable situation for potential production and biological value, therefore with special plans for culturing low demand fish would be possible. In this study, 7 fish species belongs to 7 genera from 4 families were collected. Freshwater and marine water species was observed in the catch composition. According to the results of the four studied stations, Artificial lake station and Farabi had the highest number of catch which mostly belonged to Acanttopetrus Latus and Liza Abuo. In general, based on biological parameters, such as zooplankton frequency, all stations had similar conditions but in terms of phytoplankton, Azadegan aquaculture sewage station, Pumping stations and Farabi wastewater have better condition for aquaculture. The FBI index showed that the artificial lake station and pumping station are more suitable for aquaculture activity. Low demand fish is recommended for Azadegan aquaculture sewage station. Artificial lake station had lower primary production but it is more suitable for aquaculture because of its lower FBI index value. Most of the fish presence was in the artificial lake station and Farabi wastewater which belonged to two predominant species of Acanttopetrus Latus and Liza Abuo. In general, based on biological and non-biological parameters, artificial lake station in the west Karoon and Azadegan aquaculture sewage station have better water quality than the other stations for aquaculture activities. This requires management action and special schemes.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Barbus sharpeyi is a local fish of Khouzestan that is planned to be a target for aquaculture in recent programs of fisheries organization. Considering importance of this species in its dispersal region, the main goal of this project was evaluation of possibility for triploidy induction and its potential in response to the heat shocks, efficiency of viability and growth and finally reporting the best condition for triploidy induction in Benny. Induction of thermal shocks was executed in for cold and heat shocks (2 and 4°C for cold and 34, 36 and 38°C for heat). Time of induction and its duration varied between 2 and 5 minutes after the fertilization for 3 and 5 minutes. Each treatment was repeated for 3 times. The ploidy level was determined based on size of nucleus diameters in erythrocytes. Analysis of data was done by SPSS (ver. 16) using T-test and ANOVA method. Results showed that the maximum number of triploid individuals was obtained in treatment of 38°C, 2 min after the fertilization by duration of 3 minutes but as the condition was not suitable for the viability of the eggs, losses of the larvae was high in this group. The best efficiency of triploidization in B. sharpeyi belongs to the 34°C, 2 to 5 minutes after the fertilization for duration of 5 minutes. Nuclear dimensions showed an increase in triploids and confirmed that this characteristic can be used as a reliable factor to distinguish polyploidy. Results of this study showed that B. sharpeyi has the ability for polyploidy inductions specially heat shocks. Evaluation of growth in matured fishes, use of proper tagging systems to distinguish the treatments and designing a plan for bioconserving and genetic improvement of this species is recommended.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The research conducted for finding of aquaculture potential and appropriate areas of Yamchi dam downstream river and estimate of its production in 2010-2012. Yamchi dam is located at 25 kilometers of southwest of the Ardabil city. This research done by using of available information and data obtained from the monthly and seasonally sampling. After evaluating of Yamchi river potential and its marginal lands, suitable locations for aquaculture determinated and theirs production was calculated at each site. Totally, 3 areas selected for trout aquaculture and varius method suggested including race way, octagonal pond and semi-recircultaion. In the present condition of Yamchi river the amount of trout production is predicted at least 65 ton by race way to maximum 1750 ton by semirecircultaion method. Whiles, if 2 cubic meters of water from Yamchi dam outlet is provided to aquaculture, it will be predicted at least 580 ton by race way to maximum 4000 ton by semi-recircultaion method

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: One of the most important bacterial fish diseases which has caused some outbreaks in rainbow trout farms in Iran is streptococcusis .The farmers were suffering from huge economic losses due to the disease outbreaks in different rainbow trout farms in Iran. The aim of our study was to determine rate of streptococcusis incidence in different stage of growth in farmed rainbow trout in Haraze River, Mazandaran province. Fish and water samples were randomly collected and measured of randomly in10 selected farms, monthly throughout a year. After clinical observations, Isolation and recognition of strep strains were made using biochemical tests. Some Environmental factors include Nitrate, Nitrite, Temperature, pH, Ammonia and DO measured during sampling periods. According to our results 4.6% of juvenile samples showed clinical singe of streptococcusis while only0.7% of them had strep. Contamination .These rates in adult samples were 8.9 and 1 percent respectively. Major isolated bacterial strain was Streptococcus uberis . Incidence of streptococcusis in rainbow trout 20% affected by fluctuation of Nitrite, temperature and DO. Management of these factors can decrease rate of disease outbreaks.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Mnemiopsis leidyi is one the species of comb-jelly. It belongs to the Ctenophore phylum and Lobate order. It is a purely marine, free-swimming animal with a transparent jelly-like walnut-shaped body, which was transported from the Black Sea into Caspian possibly at the end of 1990s. This species is food rival of pelagic fish. It has been reported that the ctenophore caused the dramatic decrease of zooplankton and pelagic fish stock in the Black Sea. In this study, sampling on Mnemiopsis leidyi, zooplankton, phytoplankton was conducted from December 2003 to March 2005, from a total of 11 stations located along four transects (Anzali , Khazarabad, Tourkman and Gorgan Bay) by METU net in the Iranian coasts of the Caspian Sea. Clupeonella catch was done by light fishing with fishing vessel in Anzali region. The results showed that the biomass of M.leidyi had some fluctuation in different seasons and its maximum biomass was recorded in summer with a figure of 524.4±156.2 g.m2 Tourkman region and the minimum biomass was observed in winter with a figure of 5.15±1.81 g.m2 in Anzali region. Study of stomach of Mnemiopsis showed, that Acartia (belonged of Copepoda with 66 %), lamlibranchia (13 %) has the highest frequency and the lowest were Balanus nauplii, Rotatoria, Tintinnopsis and Podon polyphemoides. The maximum mean abundance of zooplankton was 29368±24318 n.m3 in winter (Anzali region) and the minimum was recorded 4170±5014 n.m3 in summer (Tourkman region). Copepoda was dominant zooplankton in the Iranian coasts of the Caspian Sea. Chrysophyta with 150000000±440000000 n.m3 and Euglenophyta with 260000±521000 n.m3 had the highest and the lowest frequency respectively in the Caspian Sea. The main foods of Clupeonella were Acartia with 80 %. The survey of stomach of Clupeonella showed that the feeding of these species was not good and the highest fullness index was recorded 220.95±314.67. These results showed, the main food item of Ctenophora and Clupeonella were Copepoda (Acartia). It seems, the impact of Mnemiopsis leidyi feeding has been the important factor in declining zooplankton populations, Clupeonella stocks, and also increasing of phytoplankton abundance.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Streptococcosis is an acute infectious disease that causes mortality in marine and freshwater aquacultures. One of the most important hosts is susceptible to the disease of rainbow trout and during the past decade its industrial production has been growing in Iran. According to FAO, Iran is among the 10 countries producing fish in the world. The most important bacteria causing Strptococcosis includes Strptococcus, Vagococcus and Enterococcus genera. But in all cases, the clinical sings are the same. More than a decade has passed since the first report of this disease in Iran and unfortunately, this disease has become the most important problems of trout production in Iran. Therefor, 72 farms were selected in 8 provinces including Mazandaran, Gilan, Lorestan, CharmahalBakhtiyari, Fars, Kermanshah, Tehran and Kokiloye-Boyer Ahmad and a total 520 moribund and apparently healthy rainbow trout (weight 50 – 200gr) were collected during summer 2008 to 2009. Fish kidney, spleen and liver samples were culture aseptically and finally 206 isolates were identified as gram positive cocci. Using conventional biochemical test, S.uberis, S.agalactiae, S.dysgalactiae, S.faecium and S.inia. Additionally, 172 from 206 isolates were confirmed as S.uberis ، S.agalactiae ، S.dysgalactiae ، S.faecium and S.inia using a PCR assay. Based on the results, the most prevalent is belong to S.uberis (isolated from 5 of 7 province), S.dysgalactiae (isolated from 4 of 7 province), S.agalactiae (isolated from 2 of 7 province) and S.faecium and S.inia (from 1 of 7 province) respectively. It is necessary to mention, S.inia was identified just to Fars and any gram positive cocci was isolated from Tehran.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study aimed to investigate the effects of 5- parameter dissolved oxygen, PH, nitrite, nitrate and temperature of the water on Streptococcosis incidence in two groups of fry and grower fish. Research was performed at west Mazandaran province -Tonkabon region in 12 rainbow trout selected farms. Research conducted in 3 farms with well source water , 8 farms with water source of the river of Dohezar and one farm with water source of the river of Azarood, during 12 consecutive months.From 1390/04/01 to 1391/04/01, once time each month , and in each time 10 fish randomly sampled, inlet water were sampled simultaneously. Of 1350 sampled fish 607 fish with an average weight 22.04 gr , average length 12.59 cm were in fry category and 743 fish with an average weight 156.25 gr, average length 23.32 cm in were grower category. Streptococcosis observed only in grower category. Of 72 fish with clinical signs of the disease, 14 numbers were positive Streptococcosis (19.44%) and 58 numbers were negative Streptococcosis(80.55%). Three fish from grower category has not any clinical signs of disease and seemed to be healthy but were positive Streptococcosis in examinations (0.22% of total fish sampled. Fish with clinical signs of the disease but negative Streptococcosis were of at least 55.1 gr and at most weight 417 gr respectively. The results showed that 47.07% cases of Streptococcosis happened at 15.6 °C water temperature, 35.29% at 16.98 °C and 17.64% cases happened at 18.04 °C so that 100% of Streptococcosis cases was observed at the average temperature of 16.99 °C. In addition, the survey results show that despite relatively high levels of nitrite in source water of farms from wells, nitrite does not effect on the disease incidence. It seems that an optimal level of dissolved oxygen in water is effective in reducing the effectiveness of nitrite in this disease. According to equation coefficients logit model is as follows: According to Logit model, it seems that for every degree change in temperature and pH of water, morbidity change will diminish 0.37 % and 1.74 % respectively. 80.56% of fish sampled that had the clinical symptoms, was ranging from an average weight of 5.1 gr (fry) and 417 gr (grower fish), that despite having clinical signs of the disease were negative Streptococcosis. Isolation of Staphylococcus bacteria as well as Gram-negative bacilli from fish with clinical symptoms similar to the symptoms of Streptococcosis, may indicate the involvement of other pathogens in fish clinical signs.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Aquaculture is one of the most dynamic activities of agriculture sector, and it seems the most economic part. Additional needs of population to white meat and healthy protein, increase of employment, achievement of foreign exchange sources, and environmental friend ship development are basis for sustainable aquaculture worldwide. Growing rate of aquaculture within last two decades, shows highest level among agricultural products, and have been two digits for many years. Trend of aquaculture development in Iran shows same path, and devoted high share ness of the water bodies. Warm water fish which have highest share in aquaculture production, are mainly produced in freshwater. Warm water fish production in the year 1382 was 61000 tones, and increased to 167000 tons in 1392. These figures for warm water fish produced in 4025 farms with an area of 22323 ha in 1382, and developed to 14615 farms with area of 48697 ha in the year 1392. Production of this fish shows highest amount in the country, and have high economic and export values. Cold water fish, which Rainbow trout- Onchorhynchos mykiss as the main species shows additional growth in various parts of the country, so as the production in the year 1382 was 23000 tones and increased to 143000 in 1392 (one decade). The number of farms was 562 units (89.8 has) in the year 1382, and increased to 1923 units (230 has) in the year 1392, which indicates another sign of fast growing. To achieve sustainable aquaculture in aquaculture, preparation the manuals aquaculture on Best Management Practice, feed and feeding management, Fertilizations and Enrichment, Stocking methods, Pond preparations, Aerations, Site Selection and Farm Establishment, Marketing are considered , preparation of manuals for uxorious parts of production chain is essential. Site selection, Process of establishment of farm, use of mechanization for production, providing optimum oxygen levels, selection of brood stocks , are main parts of responsible aquaculture

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The study was conducted to achieve growth, survival and reproductive characteristics of four Artemia populations (A.urmiana , A.franciscana , Pakistan strain and Turkmanestan strain). In this study, The strains were cultured under the same and static environment in laboratory condition. All cysts strains were hatched using the standard methods and the nauplii from the populations were cultured in laboratory condition using 80 g/L salinity , 25±1 ºC with photoperiod ( 12L: 12D) and Dunalliella tertiolecta were fed. The growth and survival of artemia were determined on days 8, 11, 14 17 and 20 of culture. Randomly, The adult population were placed in 50 ml conical falcons and reproductive characteristics were determined. The results revealed that the survivorship A. franciscana had the highest survival (%99.8) and A.turkmanestan lowest survival (%92.1) The results revealed that the in growth rate while pakistanian strain had the highest growth rate(8554 micron). The results revealed that the reproductive characteristics in each population of species , the highest cyst production were observed in A urmiana, A.franciscana and A.turkmanestan strains and the highest nauplii production was due to pakistanian strain. The highest production were observed in A. franciscana and lowest production were observed in turkmanestan strain.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The The Shahid Rajaei Reservoir- Sari is an important and large reservoir in Iran. The major objectives of dam instruction are flood control, irrigation purposes, and electricity power. However the dam is going to supply drinking water for the people. In order to prevent threats of unsuitable water to human health risks and economic losses, it is necessary to monitor the water quality before offering it to people. In this study, some of the physicochemical parameters and Chlorophyl- a, phytoplankton,microb and fungi of Shahid Rajaei reservoir were measured at 4 stations (Shirin Roud branch, Sefid Roud branch, the crossing point of branches, near the tower) during six sampling months (June, July, August, September, November and February) in 2012-2013. In order to water quality classification, the water quality index, trophic status, Shanoon and saproby indices of reservoir calculated and the results compared to different criteria and standards. The results showed that the mean (±Standard Error) of temperature, dissolved oxygen, pH, phosohate, amonium and nitrate concentrations and Chlorophyl a were 21.35 (±1.30) ºC, 10.48 (±0.37), 8.54 (±0.04), 0.050 (±0.004), 0.036 (±0.004), 0.75 (±0.03) mg/l and 18.00 (±7.23) mg/m3, respectively. In the present study, temperature between surface and deep layer was stratified in June and July, which the stratification was registerd 0.47 and 0.69 °C decreases with increasing of each meter depth in 15 to 30 meter culumn. But, these changes for each increasing meter of water depth were 0.2 to 0.26 °C in August and September, respectively, and finally was close to zero in November. In the warm months (July, August and September) with the formation of thermal stratification in the reservoir was formed oxygen stratification, but in the cold season (November and February), with vertical mixing of water oxygen and percent saturation of the reservoir was nearly homogeneous. TSI showed the maximum and minimum values at stations 4 (oligotrophic condition) and 2 (mesotrophic condition), respectively. The maximum and minimum monthly values of TSI obtained in July, August (eutrophic level) and September, February (oligotrophic level) respectively. Based on the Water Quality Index (WQI), the reservoir was in the “good” quality in whole months, .This class shows that the reservoir is suitable as source of drinking water through routine treatment of drinking water and the quality of water is rarely is low. Meanwhile the water is suitable for swimming and water recreation and survives of .sensitive fish and other aquatic species. Based on the results, 107 phytoplankton species were identified during the period of study. The species were classified in 8 divisions. Maximum and minimum values of mean (SE) abundance observed in July and January, 661 (±286) and 10 (±2) million cells/m3 respectively at the surface layer. The One way analysis of abundance and biomass data showed temporal significant variances (P〈 0/05), however the spatial variances of data were not significant (P〉 0/05). Bacillariophyta and Pyrrophyta formed more than 95% of phytoplankton. 3 dominant species namely, Cyclotella meneghiniana, Goniaulax polyedra and Ceratium hirundinella formed about 70% of phytoplankton aboundance. Comparison of diversity indices (Shannon and Evenness) showed higher values in May and January; however the indices reached its lowest level (0.58 and 0.16) in August. Water quality assessment using Shannon index showed the lowest quality of water (moderately to high polluted) in July and August. This index demonstrated the highest water quality (slightly polluted) at station 1 and 4 respectively. The results of the water quality assessment using Saproby index (based on the resistant phytoplankton species to organic pollution) also indicated to organic pollution of water in the months of summer. The saproby assessment in stations categorized most of the stations in “moderately polluted” class of organic pollution except at station 4 which was in "slightly polluted" class. In conclusion, the removal (transfer) place and time of water to the water treatment plants.are impratnt because of temporal and spatial variation of water quality due to changes of phytoplankton structure in Shahid Rajaee Reservoir. Meanwhile, the survey showed that physico-chemical parameters alone did not reflect the actual conditions of aquatic water bodies. Monitoring of aquatic ecosystems must be complemented by biological monitoring. Microbial survey showed that the maximum and minimum geometric mean of the total number of bacteria, in September (6101559 CFU/100ml) and February (3310 CFU/100ml) respectively. However in stations, the maximum and minimum count of this parameter obtained at stations 2 (455316 CFU/100ml) and 3 (40964 CFU/100ml) respectively. There were no viable count of total coliform in the months of May and June. However it’s counting reach to the maximum value in September. Clostridium perfrigens showed viable count in water sample during September. The results also showed no proportion of fecal streptococci in microbe account in the Shahid Rajaei Reservoir. It might be good sign of suitable water quality in term of no-contamination by old and resistant fecal microbes. Base on the total coliform count, water quality was suitable for swimming and source of drinking water in most stations and months. The coliform count increased in August and September in stations 3 and 4. In these 2 months the probability of new fecal contamination increased by warm blood animals in the reservoir. It seems that the environment in September is suitable for accession of old and resistant microbes such as Clostridium perfrigens. The results of sample analysis revealed that the fungal colony counts in the station 4 and 5 were significantly higher than those the other stations. Moreover, the minimum and maximum of the fungal colony counts wereobserved in August and February, respectively. The most commonly isolated genera were Aspergillus, yeasts (especially candida) Penicillium, Cladosporium, Mucor, Fusarium, Althernariya, sterile hyafe and Paecilomyces respectively. Finally, in order to prevent of occurrence of eutrophication, algal bloom, and control of microbial activities and organic phosphorus loading it is necessary to control the activities of the human societies around the dam or the rivers tributary.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Streptococcosis is an infectious bacterial disease that causes huge economic losses in cold water aquaculture industry. Disease outbreak was experienced in some of provinces farms in recent years. Fars Province, has produced 7,000 tons of cold-water fish. According to Streptococcosis report in 1381 from the province and Proceedings have been performed during 10 years against disease and also economic losses impact of disease on rainbow trout production, risk assessment of Streptococcosis conducted on the plan. In this study, of 586 sick fish (have symptoms) studied 230 fish (39.24%) Streptococcus and (26.62%) gram negative bacteria were isolated. Of 754 healthy grower fish and fry (with no clinical signs) 10 fish or fry (1.32%)infected with streptococcus and 60 fish or fry(7.95%) infected with gram negative bacteria (Yersinia ruckeri , Pseudomonas , entrobacteriaceae).According to biochemical tests and molecular examinations , isolated Streptococcus identified as Lactococcus garvieae , Streptococcus iniae and Streptococcus sp. Furthermore some physical and chemical parameters measured and aerobic bacteria of selected farm water counted. These factors effects on disease incidence and changes were evaluated by applying logistic regression.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In this survey the Physical and Chemical factors, heavy metals,agricultural pesticides and microbial contaminations were studied in mohammadiye channel extending Eslamshahr, Robat karim and Shahriar cities of Tehran Province during the 2011.Specimens were obtained from 5 different stations .The measured factors were temperature,DO, Cl,HCO3,COD,BOD,CO2,Mg,……. The analysis of Physical and Chemical factors of Mohammadiye channel water showed that except water temperature,all factors in comparison to the standards were at the standard level,So they are suitable for aquaculture. The result of measurements of heavy metals including lead, copper,Iron,Cadmium, Zinc and mercury in spring, summer and fall in comparison to existing standards showed that unfortunately except copper the amount of other heavy metals are higher than standard levels in the most of stations , Lead amounts at stations 2,3,4 and 5 . zinc at stations 1 and 5 , Iron at stations 1,3 and 5 , cadmium and mercury in all stations were higher than the standards for fish culture. The measured amounts of organophosphate and organochlorine pesticides and result of microbial contaminations culture were less than standard levels. Due to mean of water temperature that is high from middle of Spring to the end of summer , It is suitable for culture of warmwater fishes and because of low water temperature in the station No1 It is recommended for culture of coldwater fishes by using of 50-70gr of young fishes .

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This research was performed to survey the live handling condition of Rainbow trout in Tehran province and determine the effects of this method on the quality of the flesh regarding with water binding capacity, pH changes, moisture, fat and protein content in different seasons of year. Based on reviews of Questionnaires, there were 20 main live rainbow trout sale centers in Tehran. 10 numbers of these were in fruit and vegetable centers of municipality and others were out of these centers. Live fish handling to in these areas mainly was performed from fish farms located in the Haraz road (85 percent), farms of Firoozkooh road (10 percent) and others were (5 percent) from neighboring provinces. The results of this study indicated that current methods of live handling in Tehran (the transporting time 4 h and the density of fish in tank 100-150 kg /m2) meat quality of transported trout was not affected. Water holding capacity of transported fish was less than caught fish in the fields. This amount was reached to lowest level in in summer as well as the differences between the two groups were significant (P〈0.05). Also, when a meat pH level was elevated, water holding capacity was reduced. In summer, the amount of WHC in transported fish was 13.4% that was a highest value between the seasons. Appears that in the summer, high temperature and increasing kinds of stresses to fish during handling due to excessive accumulation of metabolites such as ammonia, muscle energy sources emptied because of high activity. In this condition, falling of pH during postmortem changes being moderated and lead to increase drip loss in the transported sample.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Report of the project entitled "determination of the global population structure of the great sturgeon (Huso huso) with emphasis on the Golestan Province" is in front of your attention. Outcome is the results of study on the samples collected from the total catch in the landing sites namely Bandar-e-Anzali, Babolsar, and Ashooradeh, in the three Northern provinces. 224 specimens were collected according to the sampling manual during the fishing seasons in 2005 and 2006. Three methods were applied in this study to find out the population structure of the great sturgeon in the southern Caspian Sea. These were as follows: Morpho-metric meristic Population dynamics Population genetics (microsatellite loci) Results indicate that the range of different age groups of the great sturgeon in the Golestan Province is larger than the other two provinces. Results show that the older age groups are found in the samples from the Golestan Province rather than the other two provinces. Results demonstrate that the sample size for the two methods (morpho-metric meristic and population dynamics) is not enough. In order to obtain precise results with less uncertainty one needs to collect more specimens. Therefore, according to the few number of the great sturgeon in the total sturgeon landing, it is recommended that researchers apply methods other than the two methods which are mentioned above in order to make sure that the results are most reliable. Population genetics methods including microsatellite loci are among the methods which could produce good results with minimum bias in this regard. Results indicate that there are two distinguishable populations of the great sturgeon (Huso huso) in the southern part of the Caspian Sea off the shores of the three Northern provinces. Results also show that these two populations are scattered in the two east end (Golestan Province) and west end (Guilan Province) of the southern Caspian Sea. The two populations have overlapped each other in the Mazandaran Province.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Present project was conducted in shrimp farm located in east of Chabahar in Sistan and Balouchestan province.Twentieth sampling stations at three locations (i.e. 2 stations in supply channel,13 stations in drainage channel and 5 stations in Gwatar Gulf) were selected to determine physical and chemical factors such as; temperature, salinity, dissolved oxygen, pH, nitrate, nitrite, ammonia, phosphate and biological parameters for example Chlorophyll a, phytoplankton and zooplankton. Sampling was conducted once a month from March 2003 until January 2004. The results revealed different bioenvironmental area in the three locations (i.e. supply channel, drainage channel and Gwatar Gulf) over the study period. Concentration of salinity ,pH , nitatr ,nitrite ,ammonia and chlorophyll a in drainage channel were more than supply channel and Gawtar Gulf. While concentation dissolved oxygen and phosphate were in drainage channel less than Gawtar Gulf.Also, sediment with small grain size (clay and silt) caused uptake phosphate and amount phosphate decreased in drainage channel. Considering with lack of environment standards in Iran shrimp effluent, data compared with others countries standard. Results showed that with considerable farm area , value of temperature , salinity , dissolved oxygen and pH were in permit range in Gwatar Gulf while concentation nitrate, phosphate and chlorophyll a were sometimes more than permit range that could cause plankton bloom.Also, Gwatar Gulf were influence monsoon. Nitate , phosphate , chlorophyll a and phytoplankton increased in gwatar Gulf.Regards with that postmonsoon was simultaneus with shrimp harvest.Therefore, increase some parameters could be caused from shrimp effluent or postmonsoon. Thirty four phytoplankton genus were identified including diatoma, dinoflagellate , cyanophyta and chlorophyta. In each area maxiumum percentage of phytoplankton were belonged to diatoma( 63%) in supply channel , cyanophyta(51.7%) in drainage channel and dinoflagellate (94.5% ) in Gwatar Gulf.Amount and range chemical and physical factors and plankton composition were different in drainage channel , supply channel and Gwatar Gulf. Cyanophyta could grow more in drainage channel.Percentage of aboundance and diversity of phytoplankton changed in 2002 inrelation to years ago( before shrimp culture , 2000 year and 2001 year) specially in Gwatar Gulf.Mainly percentage of zooplankton were copepoda and thintinida respectively.Diversity zooplankton decreased ratio to years ago. In drainage channel , abundance total of phytoplankton and zooplankton were more than Gwatar Gulf and supply channel.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The marine survey for sturgeon stock assessment was conducted in summer, winter and spring in the years 2006 and 2009 to estimate the relative and absolute abundance and percentage composition of each species in the Guilan, Mazandaran and Golestan Provinces. This survey was carried out in the Iranian waters of the Caspian Sea on board the Sisara2 and Guilan vessels using trawl nets at 2-100 m depths. Trawling was carried out in 85 stations that were selected using a stratified random design. The number of stations in each scope was based on the area of the scope in terms of the total area. Trawling and sampling in shallow water up to 10 m were carried out using 9 m trawl nets whereas 24.7 m trawl nets were used for depths more than 10 m. Trawl surveys were carried out in the daytime. Trawling velocity was kept at 2.5-3 kts and trawls lasted half an hour in order to calculate abundance, and biomass of sturgeons using the swept area method. Catch per unit area (CPUA) in the winter 2006 survey was 3853 specimens nm2 , in the summer and winter 2007 survey was 1854 , 2912 specimens nm2 at depths less than 10 m respectively . CPUA for sturgeons in spring 2008 survey was 2103 specimens nm2 at depths less than 10 m and 393 specimens nm2 at depths greater than 10 m (10-100 m depth). These values in the winter 2008 survey dropped to 44 specimens nm2 at depths at depths above 10 m. CPUA for sturgeons in the spring 2009 survey was 300 specimens nm2 at depths less than 10 m and 307 specimens nm2 at depths greater than 10 m. In all the surveys conducted CPUA for A. persicus was higher than that for the other sturgeon species. Based on the calculations carried out in the marine survey in winter 2006 the estimated absolute abundance for sturgeons was about 2977.363 thousand. The total biomass of sturgeon was estimated as 131.713 tons. In the summer 2007 survey total abundance was estimated 1432.398 thousand, and total biomass of sturgeons was estimated at about 312.161 tons. In the winter 2007 survey total abundance for sturgeons was estimated at about 2250.105 thousand, and total biomass was estimated 578.08 tons. In the spring 2008 survey total abundance was estimated at about 3002.832 thousand. The total biomass was estimated at about 2533.318 tons .In the winter 2008 survey total abundance was estimated at about 152.722 thousand, and total biomass in winter 2008 was estimated 170.540 tons. Total abundance in spring 2009 survey was 1310.232 thousand and total biomass was estimated at 2019.tons. Investigation of stomach content of sturgeon Acipenser persicus caught under 10m depth in 2006 to 2007 surveys showed that there is significant difference in the consumed food. Polychaeta is the major food consumed and crustacean an the minor one(P〉0.05).Also no new types of food( such as bony fishes or benthic) have been observed in food chain of Acipenser persicus only the food consumption rate has been related to the season of year and increases or decreases in warm or cold seasons, respectively For physiological study and determination of sexual maturation stages in sturgeon,119 gonad sampling prepared. The results showed that 63 % of fishes were females and 37 % of them were males. Gill microscopic study shows complications such as hyperplasia, curvature, adhesion, embowed, shorting and lengthen of secondary filaments and fraught bloody. Microscopic study on liver indicates signs of cloudy inflammation, fatty degeneration, dispersion of billed secretions and cell atrophy. The population genetic structure of Persian sturgeon (Acipenser persicus) in Sefidrood and Gorganrood rivers watershed analyzed based on microsatellite markers during sturgeons assessment in 2006-2008. Results showed that Acipenser persicus in two region of south part of Caspian Sea are two independent populations.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Project was carried out at the Gomishan Centre for training and education which is located 15 km far from Gomishan City in the Golestan Province. Prebroodstocks were chosen from ponds (stocking density: 3/m2). 860 male and 890 female broodstocks were separated and transferred into greenhouse (400 m2). The average growth rate per day was 0.3 g and survival rate was 90%. After reaching 42 g in males and 52 g in females they were ready for Eyes stalk Brood stocks were transferred into propagation hall in June and after a week females gone under Eyes stalk. 10,890,000 eggs were yielded from 50 females and consequently 7,187,050 nauplius were produced. Each broodstock spawned 1.34Times. The maximum hetching was 75% that in was average 64.2%. Mass of Cetocerous Algae was mass produced to be fed for post larvae. Finally 35,000 PL15 were yielded. PL15 were transferred into two half hectare and one 1 ha ponds in July. The stocking dencity was 18 PL/m2. Biometric parameters together with air and water temperatures, and pH were recorded daily. Results indicate that the maximum survival rate was 58% (pond No. 5) and minimum was 52% (pond No. 17). The average weight after 101 days cultivating was 23 g which is appropriate. Also the average daily growth rate was 0.2g and the FCR was 1.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: There are many factors affecting aquaculture insurance in Iran. In spite of existing formulation for cold water fish, warm water fish and shrimp culture in Iran, there was several aspects and new revisions were remind. In this study we focused on risk assessment as well as insurance procedure to find the better management practices on running insurance in the country. 360 farm(for carp in tree provinces Guilan, Mazandaran, Khozestan),150 (for trout in tree provinces Fars, Mazandaran, Chamahal ) and all 340 farm(for shrimp in four provinces Sistan ,Hormozgan, Bushehr, Khozestan) were selected for risk analysis program. Data were collected throw questionnaire forms. The project was carried out in Two disciplines 1) risk analyzing for manageable and unmanageable accidents and their deductible rates 2) new formulation for insurance management.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Two of the three objectives of project was carried out success that one of them is following: The genetic diversity of Liza salien(Risso,1810) in the south part of Caspian sea using the Mitochondrial DNA sequencing (mtDNA) was carried out as first objective of project that based on the mitochondrial DNA sequencing (mtDNA) of 16S rRNA was used in order to clarify genetic structure and genetic diversity of lizasaliens in three western (Anzali) , middle(sari) , and eastern(Gomishan lagoon) of south part of Caspian sea. As a result we obtained 552base pairs of 16SrRNA sequence. A total of 6 different haplotypes and 29 variable sites were identified .The average nucleotide diversity(π) and haplotype diversity(h) in samples of all regions were 0.29 , and 0.004 respectively . The results obtained from genetic distance showed low rate in that of 3 regions. Estimates of gene flow indicated there is no reproductive isolation between three regions and also there was not significant genetic differentiation between differentregions (p〉0.05). the findings from the present study suggest that there is equal population of Liza saliens in the studied regions .: Genetic differences and phylogenic relationships among six Mugilidae species (Mugilcephalus, M. capito, Liza subviridis, L. saliens, L. aurata, Valamugilbuchanani) were determined using PCR-sequencing as second objective of project. M. cephalus, L. subviridis, and V. buchanani from the Persian Gulf and Oman Sea, and L. aurata and L. saliens from the Caspian Sea were collected. Samples of an imported, Egyptian species M. capito (this species was mixed with the main imported species as M. cephalusfingerling) were obtained from the Gomishan Research Center in Gorgan. Total DNA from the samples were extracted according to phenol-chloroform method Mithochondrial DNA ,16s RNA was amplified using thermo cyclermachine with universal primers and thensequenced by sending to Takapoozist Company and thereafter to France. Analysis of the sequences showed great differences between Mugil species and the other studied species. The phylogenetic tree obtained through Neighbor-Joining method revealed that L. saliensandL. aurata were in the same branch while L. subviridis was in a separate branch. In contrast, Maximum Parsimony tree located L. subviridis and L. aurata in a single branch and assigned L. saliens to a distinct branch. This result brings in the question of monophyletic origin of the genus Liza.Also.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This survey was carried out in 8 lines of southern area of Caspian sea consisting of Astara, Anzali, Sefid roud, Tonekabon, Noshahr, Babolsar, Amirabad and Torkman in depths of 5, 10, 20, 50 and 100m in 4 seasons (spring, summer,autumn and winter) of the year 2008. The goal of this survey was identification of different species, distribution, density and Biomass of the zooplankton community in different regions and also their frequency in the different layers and depths and the population fluctuations in whole of year. The most abundance and biomass of the zooplanktons were 28005±24212 ind./m3 and 180.58±149.03 mg/m3in 5 min spring and they were reduced gradually to depths.Copepoda comprised between 9 to 16 percent, Rotatoria comprised between 5 to 13 percent and Lamellibranchiata larvae comprised between 27 to 70 percent of marin zooplanktons.The most abundance and biomass were 11746±7921 ind./m3 and 47.07±31.96 mg/m3, 5281±5521 ind./m3 and 29.03±31.68 mg/m3 in 5m of summer and autumn seasons, respectively. Copepoda comprised more than 95 percent of marin zooplanktons in all of depths in these seasons. The most abundance and biomass were 19030±16518 ind./m3 and 198.99±217.23 mg/m3 in 5 m in winter. Copepoda comprised between 17 to 49 percent and Rotatoria comprised between 32 to 70 percent of marin zooplanktons in all of depths and the abundance of other groups was not considerable in zooplankton community.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The project “Stock Assessment of Zobaidy, Pampus argenteus, in the North of Persian Gulf” started in April 2003 and completed by the end of April 2006 as a cooperative research project to assess the valuable stock of zobaidy shared between Kuwait and Iran in the North of Persian Gulf. The main objectives were to investigate and determine the basic biological parameters, the standing biomass, seasonal abundance, and the impact of regional fishery activities. These studies are necessary to recommend practical management policies and measures for long-term sustainability of the zobaidy stock. The sampling of fisheries data (catch, effort, fish length distributions, and biological data) was started in May 2003 and ended December 2006. Monthly length frequencies data were collected from both the drift gill net and shrimp trawl fisheries. Age determination was based on otolith sectioning, polishing, etching, and staining method. The age groups ranged from 0 to 10 yrs but the dominant age groups were 1-3 years and the estimated parameters for both sexes were L∞ = 32.0-36.0 cm FL and K = 0.26-0.30 yr-1, females grow faster than males. Sea survey on board of two dhow boats was carried out using swept area method. Higher abundance was always obtained in Kuwait Bay rather than the other areas surveyed in Kuwait and Khuzestan waters. The total estimated stock biomass in the surveyed areas varied from 42 t in January 2004 to 2,633 t in November 2004, while the Iranian biomass varied from as low as 19 t in October 2005 to 295 t in November 2003. The data indicated that Ras Al-Gaid, Bubiyan Island is the main nursery area in Kuwait waters, while Lifah, Busaif, and Bahrakan are important nursery areas in the Khuzestan waters. Kuwait Bay and Khor Musa are considered important spawning grounds and should be protected. Yield per recruit analysis showed that higher yield could be gained with increasing fish effort, which is not recommended under the present status of high exploitation rate. In contrast, virtual population analysis indicated that future catches will reduce if the fishery continues with present level fishing effort. A reduction of 50% would be required to maintain the present level of catches as well as to enhance the stock biomass. Formulation of a joint advisory management committee would be advisable approach to manage and monitor the zobaidy stock in the region. This will need commitment from the three countries in the region for data collection on regular basis on length frequency, statistics, and biological data. Copepods with high occurence of 64% - 88% is considered as the main item in the Pomfret fish, while jelly fish and other decapods crustaceans are considered as minor items. Observation of pre- flexion stage larvae of pampus genus among the specimens collected from Khuzestan coast water could be considered as an evidence for the spawning of this fish in this region. The occurrence of pampus larvae in Khuzestan coast during the months of June to October has been reported for several times. Examination of 101 specimens (38 from Kuwait, 63 from Iran) for molecular survey does not show any significant differences between Kuwait and Iran water stocks for this fish, so it is possible to hypothesize a unit population for zobidy stock in Persian Gulf and Oman Sea as whole.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The aim of this project is to study water quality and the trophic status at different months and depths in the southeast Caspian Sea-Goharbaran based on environmental parameters and nutrients during 2013-2014 in order the feasibility of fish cage culture. Results showed that water temperature, transparency, pH, dissolved oxygen, BOD5, COD, total alkaninity and TSS were varied 9.00-29.00C, 0.50-12.00 m, 8.05-8.74, 5.76-12.85, 14-101, 21-195 and 0.00-0.12 mg/l at the different months and depths, respectively. In addition, The values of NH4+, NH3, NO2-, NO3- and PO4-3 were 0.007-0.051, 0.001-0.010, 0.002-0.015, 0.043-0.477 and 0.014-0.077 mg/l, respectively. Chl-a concentration was recorded as 0.060-8.02 mg/l. Sacled and unscaled trophic indices were equal to 3.42-5.52 and 2.61-5.85, respectively. The proper temperature for cultivation of salmonids species in this area began from October to March. The range of the standard of pH and dissolved oxygen level for fish farming cage were 7.80-8.50 and 〉5 mg /l that in current study results of pH and dissolved oxygen which were consistent in the current study. Various forms of nitrogen (ammonium, nitrite and nitrate ) at the different layers were acceptable and less than the threshold limit of Australia and New Zealand. The results of the nutrients concentrations compared with data from the reference years (1996-1996 ) in the Caspian Sea (Goharbaran region) showed that this ecosystem passed from oligotrophic status and shifted to mezotrophic and eutrophic condition. As a conclusion, although nutrients concentrations were acceptable in terms of fish farming cages in this region, some parameters such as chemical oxygen demand and transparency are not suitable for aquaculture industry. Meanwhile, based on various trophic levels, this ecosystem (Gohrabaran region) has a mezotrophic status with a high risk of eutrophication.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In this report rules and focal points about code of conduct for cold water farm, warm water establishment, mechanization management and oxygen generators are collected and discussed. One of the most important point difference between aquaculture engineering with mechanization. Mechanization is improvement of management level from ancient to better by some special tools but aquculture engineering can do by any vessel in every management.

Description: Iranian Fisheries Science Research Institute

Description: Published