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  • Iran  (739)
  • Fisheries  (414)
  • Inorganic Chemistry
  • Iranian Fisheries Science Research Institute  (804)
  • California Department of Fish and Game, Marine Resources Operations  (141)
  • Inter-American Tropical Tuna Commission
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  • 101
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25487 | 18721 | 2018-10-02 10:24:03 | 25487 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The Regulatory Roadmap for Health and safety of fishery products is a strategy to produce a sustainable regulatory future that meets the objectives of protecting the Iranian public from the sale and advertising of unsafe fisheries health products, and supporting the safest consumption of fish and use of health fishery products. fishery The Roadmap provides the vision to transform nearly a dozen current frameworks for fish and health that are of various ages and regulatory approaches into an efficient, transparent, and aligned regulatory products health fisheriessystem that contributes directly to the safety of I ranian’s and the benefits they gain from fish and ll also be cognizant of the previous work accomplished by the Department, which As a strategy, it wi products. -health product or fish over its full life fisheriesrecognized that there is a progression in the knowl edge about a sociated with the benefits and harms of a product cycle. This increase in knowledge reduces the uncer tainty as As a consequence, it is logical to regulate in a and can lead to improved health outcomes for Irania n’s people. cycle.-manner that reflects the full life meaningful than ever, want transparency The Roadmap also acknowledges that Iranians, now mo Key to promoting meaningful transparency (beyond just access to information online) is taking transparency. providing leadership, being honest, taking action to engage s takeholders and the public, showcasing sources, and The ability of the regulator to gain and disseminate packaged) information.-good (not only new or pre information will be complemented by the obligations placed on regulated parties. With this vision and guiding principles, the National roadmap for health and safety of fishery products lays out the way to move from the old frameworks to the new regulatory system. This will require the sequencing of a number of amending initiatives, some staged in the near future and others implemented in the longer term. While aiming for the eventual comprehensive amendment of all regulatory frameworks for food and health products under the Food and Drugs Act, early emphasis will be placed upon amendments that will deliver: The clearest value to Iranian’s and the health care and food safety systems. The greatest efficiency by cutting through unsustainable administrative requirements or approaches and replacing them with ones drawing upon international partnering, best practices and new technological advantages to contribute directly to the safety of food and health products. Building upon these early initiatives, the resetting of the frameworks will be accomplished through a series of more comprehensive amendments over the next years. The benefit to those in the National roadmap for health and safety of fishery products is to have: operational sustainability; international alignment; and the enabling of innovation and excellence in science to improve health outcomes. The National roadmap for health and safety of fishery products is structured into three parts: Where we are: provides an understanding of the existing regulatory frameworks (for fish and fisheries health products, including pharmaceuticals, natural fish health products, biologics, veterinary drugs, industrial devices) that are to be transformed. Where we are going: provides the objectives and an outline of the new regulatory system for implementation. How we will get there: guides discussion on the planning and sequencing of amendments over the next years, both short and long term, to best achieve modernization for the National roadmap for health and safety of fishery products.
    Keywords: Aquaculture ; Iran ; Fishery ; Roadmap ; Pharmaceuticals ; Biologic ; Veterinary drugs
    Repository Name: AquaDocs
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  • 102
    Publication Date: 2021-07-16
    Description: At the present study, the environmental pollutants such as organochlorine pesticides (OCPs), heavy metals (during three seasons) and surfactants (during four seasons) were done 2009 to 2010 that located in Mangol Dam with longitude and latitude 52° 22' 49" N and 43° 15' 43" E, respectively. The aims of this study were to determine the seasonal pollutants matters in water samples of under-groundwater and surface water in 12 stations and the results are as follow: The maximum residues fluctuations of OCPs were determined in between all water samples such as aldrin and endrin about 1.51 and 2.85 µg/l, respectively. The mean concentration of aldrin and endrin about 0.66 ± 0.59 and 0.71 ± 1.07 µg/l, respectively were determined. The maximum seasonal concentration of OCPs were detected in fall, winter and spring for such as endosulfan sulfate, γ-BHC, heptachlor, endrin aldehyde compounds about 2.85, 0.34, 0.14 and 0.14µg/l, respectively. The maximum seasonal percentage range of OCPs were detected in fall, winter and spring in water samples such as endosulfan sulfate, DDD and β-BHC were 42, 25 and 25 %, respectively. The maximum seasonal concentrations of Zn and Fe elements were detected in spring about 1.12 and 8.22 µg/l, respectively. Also for Hg in spring was determined about 17.2 mg/l. The mean concentration of Zn, Fe and Hg in water sampled were 0.23±1.01 and 1.21±2.00 µg/l, respectively and 4.65±6.38 mg/l. The maximum seasonal percentage range of heavy metals were detected in fall, winter and spring in water samples such as Ni, Zn and Fe were 66, 83 and72%, respectively. The maximum seasonal concentrations of heavy metal were detected in Sorkhroud and Polechelave stations during fall, winter and spring for only Fe about 1.48, 3.3 and 8.22 µg/l, respectively. The maximum concentration of linear alkyl benzene sulfonate (LAS) in last month of summer was 971µg/l. The mean concentration of surfactant during fall, winter, spring and summer was 418.62±52.16 µg/l. The maximum seasonal percentage range of surfactant uring fall, winter, spring and summer in all stations of sampling were 76, 76, 100 and 100 %, respectively.
    Keywords: Pollution ; Iran ; Mazandaran Province ; Mangol Dam basin ; Haraz River ; Sorkhroud river ; Organochlorine Pesticides ; OCPs ; Heavy Metals ; Surfactants ; Environmental Pollution ; Suface water ; Underground water ; Wastewater ; Samples ; Sampling
    Repository Name: AquaDocs
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  • 103
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25494 | 18721 | 2018-10-05 07:36:19 | 25494 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Certain diseases such as bacterial , viruses, parasirtic ones Protozoa parasites Could severe mortalities in farmed shrimp last decades. Two hundred and seventy Fenneropenaeus indicus with the weight of 10± 1.09 were selected, transferred to Iran Shrimp Center and distributed to the treatmentsand controls (3 groups). The objective of our study was to describe the signs, prevalence, and intensity of Hemocytosis, a new disease in shrimp, Fenneropenaeus indicus. The experimental design included three groups, one treatment as infected shrimp or group B and 2 controls with non-infected but exposure to White Spot Syndrom Virus (WSSV) shrimp as group A and non-infected non- exposure to WSSV shrimp as group C in triplicate. The results revealed that Hemocytosis associated with a decrease in hyalinocytes and large-granulocytes (less than 8%) and a considerable increase in semi-granulocytes in group A and B in contrast to group C that Hyalinocyte increased. This infection made the shrimp susceptible to WSSV disease. It is believed that Hemocytosis causes a condition which can result in a rapid mortality among susceptible species, F. indicus when exposure to WSSV is occurred.
    Keywords: Health ; Iran ; White Spot Syndrome Virus ; Fenneropenaeus vannemai ; Challenge ; Protozoa ; Shrimp ; Fenneropenaeus indicus ; Bacterial ; Parasites ; Mortalities ; Hemocytosis ; Hyalinocyte ; Species ; F.indicus
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  • 104
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25493 | 18721 | 2018-10-05 07:06:42 | 25493 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Out put of sturgeon fish hatcheries on the Northern provinces analyzed. Unfortunately, for many reasons such as decreasing of brood fishes ,unsuitable and unprofessional managements, lack of sufficient financial supports, ageing of laboratory and incubation instruments, unemployment of energetic expertise sturgeon propagation and restocking programmes get decreasing trends. Thus managers must planning for activating of sturgeon restocking and propagation subjects.
    Keywords: Aquaculture ; Iran ; Alborz region ; Sturgeon hatchery ; Unsuitable management ; Propagation ; Restocking programme ; Sturgeon ; Brood fishes
    Repository Name: AquaDocs
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  • 105
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25506 | 18721 | 2018-10-05 07:29:35 | 25506 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Seafood business is one of the important subjects in fishery activation. It is covered a wide range of fishery activation from the fishing to the table. The fishery production success is depending to the many factors. Without considering the market conditions and suitable trading the fishery activations are not commercial. Therefore considering to the different subjects is necessary. This project is one of the road map seafood processing plans. The aim of this project was to study of the world fishery production, world fish processing, marketing and fish production and treading in Iran by using current different information.
    Keywords: Fisheries ; Iran ; Fishery ; Road map ; Seafood
    Repository Name: AquaDocs
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  • 106
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25174 | 18721 | 2018-09-03 17:15:43 | 25174 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The population genetic structure of five Caspian Sea sturgeon species was investigated. Totally 1121 samples of caudal and unault's fin tissue of the sturgeons (Acipenser persicus, A. gueldenstaedtii, A. stellatus, A. nudiventris and Huso huso) were collected from the Volga River (Russia), Ural River (Kazakhstan), Kura River (Azerbaijan), Sepidrud River and the coastline of the south Caspian in the Iranian waters as well as from the sampling stations selected for the marine survey for sturgeon stock assessment in the Caspian Sea. All samples were stored in 96% ethyl alcohol and transferred to the genetic laboratory of the International Sturgeon Research Institute. Genomic DNA was extracted using phenol-chloroform method. The quality and quantity of DNA was assessed by Agarose gel (1%) electrophoresis and spectrophotometry. The population genetic structure of Ship and Persian sturgeon was studied using both PCR-RFLP (D-loop and ND5/6 gene) and microsatellite technique and that of H. huso, A. stellatus and A. persicus were studied using microsatellite technique. After amplification of genes using PCR, the RFLP technique was used to digest mtDNA using restriction enzyme. The PCR products were electrophoresed on 6% sequencing polyacrylamide gels followed by silver nitrate staining. Data for PCR-RFLP were analyzed using REAP program and those from microsatellite technique were analyzed using Gene Alex. Population genetic parameters including allele frequency, expected and observed heterozygosity, effective allele, Shannon's index were determined. Genetic identity and distance were calculated following Nei criteria and Hardy Weinberg equilibrium was tested based on X2 and analysis of molecular variance (AMOVA) using Reap and Gen Alex at 99% confidence limit. Phylogenetic relationship was determined and drawn using TFPGA program. The population genetic structure and genetic diversity of the 1121 sturgeon specimens were determined. Three independent populations were identified for Acipenser persicus (two populations in the south Caspian in the Iranian waters and one in the north Caspian). Three independent populations were identified for A. gueldenstaedtii (Volga, Ural and South Caspian populations) using the microsatellite technique. Population genetic structure using PCR-RFLP revealed no genetic differentiation among the A. gueldenstaedtii specimens studied from the different regions using ND5/6 gene, while two populations (Ural and south Caspian populations) were detected for this species with the same technique using D-loop genes. Four independent populations (Volga, Ural, Kura and Sepidrud populations) were reported for A. stellatus using the microsatellite technique and four more populations which most probably belong to the autumn and spring races of the above mentioned independent populations were identified for this species. The present study also identified two populations for H. huso; The North Caspian population (in Volga and Ural Rivers) and The South Caspian population (in Golestan and Guilan regions) which were significantly different from each other (P〈0.01). The genetic population structure of A. nudiventris was studied using the microsatellite and PCR-RFLP techniques which revealed two populations for this species one in the Ural River and the other in the Sepidrud River (South Caspian). Comparison of the ND5/6 and D-loop genes studies in Russian sturgeon revealed that the D-loop gene is better than the ND5/6 genes in population's differentiation and is therefore strongly recommended for population genetic studies on sturgeons in the Caspian Sea. Genetic diversity studied using microsatellite technique was higher and more accurate as compared to that using RFLP. Nevertheless the RFLP technique was able to introduce molecular markers for the population’s species pacific identification. On developing suitable primers these studies can be speeded up and the cost of such studies can be cut down. However the drawback in using microsatellite technique for population genetic studies is that it cannot introduce a molecular marker for the identification of populations. The present study was able to introduce molecular markers to differentiate the ship sturgeon population in the south Caspian from that in the Ural River using the PCR-RFLP technique Based on the results obtained it is strongly recommended that all activities related to restocking and rehabilitation of sturgeon stocks in Iran be conducted on the basis of genetic principles. Also serious and immediate measures should be taken for the restoration and conservation of rare population of native species of Iran particularly in the Sepidrud region using genetic markers before they are become extinct.
    Keywords: Biology ; Iran ; Caspian Sea ; Guilan Province ; Golestan province ; Sepidrud River ; Assessment ; Sturgeons ; Population ; Genetic ; PCR-RFLP ; Microsatellite ; Species ; Samples ; Tissue ; Acipenser persicus ; A. gueldenstaedtii ; A. stellatus ; A. nudiventris ; Huso huso ; Survey
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  • 107
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25178 | 18721 | 2018-09-03 14:20:19 | 25178 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Pin bone removing is a new equipment for pin-bone removal increase customer satisfaction and revenue even further the bones were pulled out at good speed. Pin boning especially is very often done manually which causes lots of global transports to low cost countries for processing new technique will help make processing operations more efficient and profitable The new generation pin bone removing equipment is mainly for trout and is not suitable for carp fishes the new pin bone remover which works with air of compressor and hand. has proved to work in almost twenty different fish species, including Great silver smelt, Pike Perch, Coho, Sockeye salmon, Atlantic Salmon, Sea Trout, Saithe, Haddock, Herring, Whitefish, Chinook, Salmon, Perch, Rainbow Trout, Char, Mackerel and Hake. Therefore it will be suitable for carp fishes too. After a day’s work all vital parts can be removed by hand without any tools, for effective cleaning to the fish and gives nice looking filets and is determined to help processors by offering state of the art bone-removing equipment which will increase speed, yield and efficiency. But pin bone removing done manually does not give a nice looking fillet also has a considerable waste of fish.
    Keywords: Fisheries ; Iran ; Silver Carp ; Fillet ; Bone ; Generation ; Species ; Pike Perch ; Coho ; Sockeye salmon ; Atlantic salmon ; Whitefish ; Rainbow Trout ; Evaluation ; Great silver smelt
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  • 108
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25176 | 18721 | 2018-09-03 17:05:47 | 25176 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In the early 1980s, an alien ctenophore Mnemiopsis leidyi already known as a gelatinous zooplankton was transported (likely via ballast waters) to the Caspian Sea from its introduced or native water of the Black Sea or western Atlantic which caused negative impacts. In this report, distribution of M. leidyi, planktonic and benthic organisms had been investigated in the Southern Caspian Sea (Iranian waters) in 6 transects consisted of Lisar, Anzali, Sefidroud, Nowshar, Babolsar and Amirabad at 26 stations during 2005 and 2006. As a whole, 1422 samples had been analyzed included 258, 346, 217, 117 and 484 belong to ctenophore, phytoplankton, zooplankton, benthos and physicochemical parameters, respectively. M. leidyi abundance and biomass were fluctuated between 284-2751 ind.m^-2 and 16.9- 390/9 g.m^-2 respectively in 2005 while maximum abundance and biomass were recorded in autumn and minimum in winter. In 2006, its mean abundance and biomass were 184-2150 ind.m^-2 and 9.1- 209/8 g.m^-2 respectively. M. leidyi maximum abundance and biomass were noted in summer and its minimum in spring and winter months. Overall, mean M. leidyi abundance and biomass were recorded in 20 m within 1202 ind.m^-2 and 139.5 g.m^-2 respectively. Maximum size of the ctenophore was recorded as 55 and 60 mm in 2005 to 2006 respectively, while less than 10 mm length frequency consisted 83.16 and 82.88 per cent of total population. Spatial_ temporal distribution of M. leidyi in different regions showed it was more abundant in west and east (714- 4494 ind.m^-2) ratio to central parts (13-1519 ind.m^-2) of the Southern Caspian Sea. Species composition of zooplankton had shown the negatively impacts of M. leidyi invasion in the period of sampling as the only 17 holozooplankton were determined with Rotatoria (7 species), Copepoda (4 species), Ciliophora (4 species) and cladocera (1 species). Zooplankton maximum abundance and biomass were recorded in February (3039742901 ind.m^-3 and 372.1575.4 mg.m^-3) respectively and its minimum were in July (12211601 ind.m^-3 and 6.9 7.7 mg.m^-3) respectively which was belonged to Rotatoria (76%) and Copepoda (10%). In this study, 21 species of benthic fauna were deter minted belong to Pseudocumidae (5 species), Gamaridae (4 species) Amphartidae (3 species), Oligochaeta, Balanidae, Xantidae, Nereidae, Scarbicularidae, Carididae, Mytilidae, Herpobdellidae and Chironomidae (each 1 species). Oligocheata was recorded with maximum abundance (943 2502 ind.m-2) and Bivalve consisted of higher biomass (68.7162.5 g.m^-2). From five phyla of phytoplankton, 172 species were determined of Chrysophyta (74 species), Cyanophyta (32 species), Chlorophyta (32 species), Pyrrophyta (24 species) and Euglenophyta (13 species), in which Chrysophyta and Pyrrophyta were noted as main groups with their maximum abundance (up to 65%) and biomass (93%). Maximum water temperature was recorded in August with 29.52.5 ֯C and minimum in February by 9.31.3 ֯C. Mean value of salinity was 12.010.90 ppt, secchi disk ranged between 0.2 to 7 m and oxygen demand varied from 5.95 to 10.54 mg. l^-1). Moreover, silicate concentration was recorded between 200 to 300μg.L^-1, Phosphate measured from 31-47 μg.L^-1, Ammonia varied in 10-29 μg.L^-1, Nitrite (0.6- 1.7 μg.L^-1), Nitrate (0.06-4.20 μg.L^-1, which mainly accumulated in coastal waters of the southern Caspian Sea.
    Keywords: Ecology ; Iran ; Caspian Sea ; Anzali ; Sefidroud ; Nowshar ; Babolsar ; Mnemiopsis leidyi ; M.leidyi ; Benthic ; Organisms ; Phytoplankton ; Zooplankton ; Benthos ; Gamaridae ; Amphartidae ; Oligochaeta ; Balanidae ; Xantidae ; Nereidae ; Scarbicularidae ; Carididae ; Mytilidae ; Herpobdellidae ; Distribution ; Species ; Biomass ; Abundance
    Repository Name: AquaDocs
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  • 109
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25179 | 18721 | 2018-09-03 17:23:17 | 25179 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study two species of algae, Sargassum glaucescens and Padina boergeseni that found plenteously in Persian Gulf and Bushehr coast, were collected and hot water extracts of them were lyophilized. F. indicus (11.32±1.20 g), after two weeks adaptation in Shoghab research station were immersed in seawater (39 ppt and 25±1 ○C) containing hot-water extract of each brown algae, S.glaucescens and P.boergeseni, at 100, 300 and 500 mg/l concentration, Survival rate and immunological parameters (total haemocyte count (THC), total plasma protein (TPP), Phagocytic activity, bacterial clearance efficiency and bactericidal activity) were examined. In addition effect of dietary administration of beta 1, 3 1, 6 glucan on prevention of White Spot Disease and immunological parameters of shrimp were investigated. According to results, immersion in seawater containing 300 and 500 mg/l concentration of algal hot-water extract after 2 and 3 hours or oral administration of beta 1,3 1,6 glucan at level of 10 g/kg diet for 14 days significantly enhanced THC, TPP, Phagocytic activity, bacterial clearance efficiency and bactericidal activity. Immersion in seawater containing 100, 300 and 500 mg/l hot-water extract of S.glaucescens after 3 hours, improved the survival rate of WSSV-infected F. indicus.
    Keywords: Biology ; Chemistry ; Iran ; Persian Gulf ; Bushehr Province ; Beta-glucan ; Sargassum ; Padina algae ; WSD ; Fenneropenaeus indicus ; Species ; Algae ; Brown Algae ; S.glaucescens ; P.boergeseni ; Survival rate ; White Spot Disease ; Shrimp
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  • 110
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25183 | 18721 | 2018-09-03 17:12:18 | 25183 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Possibility of rearing silver bream (Sparidentex hasta) in the earthen pond was experimented using 2 types of available artificial trout feed (FFT and GFT1). The experiment was carried out in 2 phases in Shahid-Kiani Extension Shrimp Farm in Chowibdeh Shrimp Site-Abadan Southern Iran, and earthen ponds with 0.25 ha. Area were used for both phases of the study. Juvenile fish of 3.2 g initial weight with the density of 9324 ind./ha. Were stocked for 196 days in the first phase. The initial weight, stocking density and rearing period of the fish in the second phase were 108.60 g, 9800 ind./ha. And 237 days respectively. Maximum amount of WG, FCR, PER and SVR of the fish in the first phase of the study were 100.12 g, more than 6, 0.38 and 55.32% respectively. WG and SR of the fish in the second phase respectively were 417 g and 20.74%. The Salinity of pond water was in the range between 9 and 22 ppt, and the water temperature was between 8.3 ֯C (mid December morning) and 34.8 ֯C (early Sep.....
    Keywords: Aquaculture ; Iran ; Abadan ; Chowibdeh ; Cultuation ; Silver Bream ; Sparidentexhasta ; Earthen Pond ; Artificial feed ; Rearing ; Shrimp ; Juvenile ; Weight ; Density ; Temperature
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  • 111
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25177 | 18721 | 2018-09-03 17:18:50 | 25177 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Fish cell lines have been used to support many areas of research, beginning with fish viruses and extending into immunology, genetic studies, toxicology, environmental effects, aquaculture and seafood quality and it is the first step in the gene banking, to preserve gene materials. The present study we cultured cell of interior pituitary of the Acipenser gueldenstaedtii gland, as a first attempts in IRAN. FISH cell culture has widespread applications in virology, toxicology and as in vitro models in cytogenetic, biomedical, physiological researches. A cell line has been established from the Acipenser gueldenstaedtii interior pituitary and scales have been used to develop primary cell cultures. Recently, successful primary culture of the interior pituitary gland of Acipenser gueldenstaedtii has been developed by explants method. The present study evaluated the potential of several interior pituitary gland from different developmental stages for development of cell cultures using explants method. Pituitary gland from various stages of the adult Acipenser gueldenstaedtii was collected under standard aseptic conditions. Developing gonads from 15-20-year-old male and female sturgeon were collected during late April and early May2007. In all the cases the tissues were pooled in cold PBS antibiotic antimycotic solution (Sigma Chemicals, USA). The tissues were evaluated for attachment, growth and ability to undergo to produce suspension of cells. Primary cultures were initiated from the above tissues according to our earlier procedures, with certain modifications in the sub cultivation procedure. Briefly, tissues were cut into 1 mm3 size fragments which were seeded into 25 cm2 tissue culture flasks. After appropriate semidrying and addition of minimum essential medium (MEM) (Sigma, USA) supplemented with 15% fetal bovine serum (FBS) (Sigma, USA), cell growth was monitored. A seeding density of 1.5 105 cells as determined by a hemocytometer. The results have been showed the Acipenser gueldenstaedtii interior pituitary cells growth in incubator Co2 in 370 C, the cells adapted in this temperature. They were in Lag phase for 10 days, in log phase on 10- 22 days, and in stationary phase on 23- 28 days, after that they died. So we could produce sturgeon growth hormone from fish pituitary cells culture. By this study we can passage the cells on 21th day, for every week. In this way we can produce continued cell culture and store them for gene banking.
    Keywords: Biology ; Iran ; Acipenser gueldenstaedtii ; Pituitary gland ; Primary cell culture ; Fish
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  • 112
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25519 | 18721 | 2018-10-05 15:40:50 | 25519 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The Persian sturgeon (Acipenser persicus) is more abundant sturgeon species in the South Caspian Sea and consist the highest proportion of Iranian Caviar, meat as well as bringing maximum foreign currency income, however from systematic point of view and differentiation of this species from Russian sturgeon (Acipenser gueldenstadttii) a serious challenging issues remain, where some Russian scientist are believe that the Persian sturgeon is not as an valid species and consider it as a subspecies of Russian sturgeon. This research conducted with the objective of identification and introducing a molecular marker based on specific DNA for differentiation of two species of Persian sturgeon and Russian sturgeon via a proved molecular marker method. For this purposes 8 different molecular approaches such: Microsatellite, AFLP, RAPD, sequencing of Cytb, 16sDNA, ND5, Growth Hormone gene and finally Single Nucleotide Polymorphism (SNP) were investigated. Based on applied methodology, between 5 to 16 caudal fin tissues were sampled for each species from different region of the Caspian Sea, Sefiedrud River, Ural and Volga rivers. Following DNA extraction, its quality and quantity were determined and the PCR experiment has been conducted using 5-110 primers according to various methods and type of gene. The PCR products were electrophoresed on Polyacrilamid or agarose gels and followed by silver and Ethidium Bromide staining. In RAPD method, polymorphic DNA band was cut on the gel followed by purification and then the segments were cloned in vector in Top10 strain of E.coli, and then sequenced. Meanwhile for Growth Hormone gene in Persian and Russian sturgeon the MEGA 4, Gene runner software were used to design the appropriate primers for PCR amplification. The PCR products were cloned in PTZ57R/T vector and transformed in Top10 E.coli strain and sequenced finally. For all other genes, similar methods were applied for PCR amplification and its products were sequenced and statistical analysis as well as phylogenetical tree was performed. In Single Nucleotide Polymorphism (SNP) method, after genomic library construction, in total 14.4 billion nucleotides were sequenced and similarity/ differentiation analysis of two species were investigated using specific bioinformatic software. Results indicated that Microsatellite and AFLP methods showed high level of genetic variation both within and between species. The Cytb gene, when 4 sample sequences from each species were compared two species were differentiated, however when analysis repeated over 15 samples, the sequence comparison couldn't differentiate two above mentioned species. Full sequence comparison of 16sDNA and mtDNA-ND5 gene showed variation in some nucleotide in both species of Persian and Russian sturgeon but no significant. Results of sequences obtained from cloned segment with RAPD method and also specific primer design based on produced sequences could succeed to discover a variable DNA band that able to differentiate two species from each other. Results of the present study also showed that the growth hormone gene (GH) of Persian and Russian sturgeon consists of 645 nucleotide that translate to 214 Amino Acids. The sequence comparison indicated that the gene coding growth hormone in Persian and Russian sturgeon had the highest similarity with GH of Mammals (71%), Anguilaformes (63%) and less similarity with bony fish (37%). Phylogenetic analysis indicates that Persian and Russian sturgeon in compare to other organism are ancient species and this gene is originated from a common ancestor. At present study the most appropriate results obtained from Single Nucleotide Polymorphism (SNP) method by sequencing 14.4 billion nucleotide from genome of two species of Persian and Russian sturgeon from North and the South Caspian Sea could prove that the Persian sturgeon is a valid and independent specie. This excellent results is the biggest scientific achievement for differentiation of two highly commercial important sturgeon species in the Caspian Sea in last two decades.
    Keywords: Biology ; Iran ; Caspian Sea ; 16S rDNA ; Acipenser persicus ; Persian sturgeon ; Acipenser gueldenstadttii ; DNA ; Russian sturgeon ; Species differentiation ; Molecular Marker ; SNP
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25510 | 18721 | 2018-10-05 07:44:21 | 25510 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this survey the Physical and Chemical factors, heavy metals, agricultural pesticides and microbial contaminations were studied in mohammadiye channel extending Eslamshahr, Robat karim and Shahriar cities of Tehran Province during the 2011.Specimens were obtained from 5 different stations .The measured factors were temperature, DO, Cl, HCO_3, COD, BOD, CO_2, Mg,... The analysis of Physical and Chemical factors of Mohammadiye channel water showed that except water temperature, all factors in comparison to the standards were at the standard level, So they are suitable for aquaculture. The result of measurements of heavy metals including lead, copper, Iron, Cadmium, Zinc and mercury in spring, summer and fall in comparison to existing standards showed that unfortunately except copper the amount of other heavy metals are higher than standard levels in the most of stations , Lead amounts at stations 2,3,4 and 5 . zinc at stations 1 and 5 , Iron at stations 1,3 and 5 , cadmium and mercury in all stations were higher than the standards for fish culture. The measured amounts of organophosphate and organochlorine pesticides and result of microbial contaminations culture were less than standard levels. Due to mean of water temperature that is high from middle of Spring to the end of summer, It is suitable for culture of warmwater fishes and because of low water temperature in the station No1 It is recommended for culture of coldwater fishes by using of 50-70gr of young fishes .
    Keywords: Aquaculture ; Ecology ; Iran ; Mohammadieye channel ; Physical and Chemical factors ; Heavy metal ; Agricultural pesticides ; Microbial contamination ; Aquaculture ; Monitoring ; Water
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25522 | 18721 | 2018-10-05 08:01:41 | 25522 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Carp culture in extensive and semi-extensive systems: i.e., earthen ponds, natural and semi-natural water resources, reservoirs and the paddy field has widspred distribution. Indian major carps including Catla (Catla catla), Roho (Labeo rohita), Mrigal (Cirrhinus mirgala) which have faster growth and good feed value than other warm water fishes introduced to many countries including India, Thailand, Burma, Philippines, Japan and the former Soviet Union are also considered and are reared. Sex steroids are important in the control of reproduction in fish. Development of methods for Indian education programs for proliferation requires knowledge of the hormonal changes during sexual maturation and spawning is. Testosterone, progesterone and 17 ϐ-estradiol are steroid hormones that play an important role in controlling Tuesday reproduction and sexual maturity of the fish are. This study aimed to investigate the changes in steroid hormones testosterone and 17 beta-estradiol including Catla (Catla catla), Roho (Labeo rohita), Mrigal (Cirrhinus mirgala) were conducted in different seasons. 40 specimen of carps breeders were investigated in southern (Aquaculture Research Institute) and north (North Aquaculture Research Institute) of Iran and maintained in different seasons (spring, summer, autumn, winter). Fish were caught by netting vetch and spring 1 cm. Blood samples were collected from the fish caudal blood serum by centrifugal separation model Labofuga 200 was made in Germany. Testosterone, and estradiol RIA (Radioimmunoassy) using an automatic gamma counter LKB model made in Finland made in France using the Immunotech kit hormone were measured. The results showed that the average level of 17 betaestradiol in the female in the spring, summer, autumn and winter, respectively, 82/12 ± 75/107, 66/13 ± 2/80, 73/17 ± 8/122 and 72/17 ± 25/104 ml, respectively. Mean testosterone levels in the female in the spring, summer, autumn and winter, respectively, 004/0 ± 092/0, 002/0 ± 05/0, 003/0 ± 11/0 and 006/0 ± 1/0 ng ml, respectively. Overall, the highest levels of 17 beta-estradiol and testosterone in female Roho were recorded in autumn. Also, low levels of 17 beta-estradiol and testosterone in female Roho was observed in summer. Highest and lowest levels of the male hormone, respectively, were recorded in winter and spring. The relationship between the hormone 17 beta-estradiol and testosterone with environmental factors such as pH and dissolved oxygen were discussed. there was a positive correlation between testosterone levels in males only the amount of dissolved oxygen .results revieled that sex hormone levels were increased during winter and autumn would be the signe for reproductive performance and spawning seasone in three species .
    Keywords: Aquaculture ; Iran ; Catla catla ; Labeo rohita ; Cirrhinus mirgala ; Carp culture ; Spawning ; Adult ; Indian Carp ; Earthen ponds
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25520 | 18721 | 2018-10-05 07:57:39 | 25520 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this survey, hydrology and hydrobiologycal studies in the northern part of the Oman Sea and Strait of Hormuz within the Iranian waters were conducted during 2007 and 2009.The project was implemented using Ferdous research vessel.The sampling area included the whole stretch of the Iranian waters along the northern part of the Oman Sea from the northeast of the Iranian marine border of 30 miles Bay of Chabahar to the mouth of the Hormuz Strait in Hormuzgan province. Along this stretch 10 equally distributed transects at a distance of 30 miles away from each other were designated, 4 main sampling stations at a distance of 10 miles from one another were fixed at each transect. Sampling was conducted twice a year (spring and autumn 2007 and 2009). According to achieved results; we can point out to the following outlines below: permanent thermocline exists annually in this body of water; where only the point of start, and the fracture width of thermocline layer; alter with alteration of seasons. Horizontal and vertical distribution of electrical conductivities, obey exactly from thermal degree structure.Level of salinity increase from east to west, but it decrease; from surface to deeper layers. In deeper regions, especially in the midway east of the Oman Sea; the level of salinity in the deeper waters from 150-300m, there is a considerable increase observed in salinity, due to very high salinity waters of The Persian Gulf; that are entering the Sea of Oman, via the Striate of Hormouz from the bottom; where they are having their effect following the whole length of the Sea of Oman; through their path. The level of water density from offshore to onshore, and from surface to deeper levels increase in such a way that. The level of Chlorophyll-a in surface waters, in the northeastern midway; is higher than the northwestern midway of the Sea of Oman, and it decreases from inshore to offshore waters, but its vertical distribution; has caused somehow in a way, that the highest distribution and concentration of chlorophyll-a; to be formed in deeper layers, between about 10-40m depths. Then by the increase in water depth, its concentration decreases drastically; reaching zero in magnitude. Concentration of dissolved oxygen is higher in spring in comparison to that of autumn, and the highest level is achieved in water layers located at 10-40m depth (where the level of chlorophyll-a is higher). In addition to which, that its vertical structure; shows the existence of a permanent oxycline layer at this region, in a way that; by seasonal alterations (similar to that of thermocline layer), only the point of start and that of the thickness of oxycline layer changes.pH level decreased from water surface, and its vertical alteration trend; obeyed to that of the same for temperature and chlorophyll-a vertical structure, especially for dissolved oxygen parameter, where the maximum decrease was recorded coinciding with formation of oxycline layer.Nutrient levels increased; at surface water layers from offshore to inshore, and from west to east, for the same body of water. In addition to that, concentration of nutrients in autumn (after monsoon); were more than that of, spring season (before monsoon), where; concentration of their vertical structure increase from water surface toward deeper sections. In this survey six phylum of phytoplankton including in Bacillariophyta (88) Pyrophyta (111), Cyanophyta (6), Chropmophyta (2), Euglenaphyta and silicoflagellate (1) were identified. A result showed that density decreased in most transects from inshore toward sea and maximum density was in photic layer (0-25m). Phytoplankton densities (without Cochlodinium density) in post-monsoon were recorded higher than pre-monsoon, furthermore phytoplankton density in 2009 was more than 2007(6073±1038) Species richness was indexed in the pre-monsoon and post-monsoon 2007 (0.337-0.519), (0.296-0.396) respectively and to (0.967-1.525), (1.407-1.531) for 2009. Zooplankton population in this study is characterized by eight phyla, eight classes, 15 orders, 35 families and 78 genus. In 2007, the pre-monsoon percentage of groups were frequently as following: Copepoda with stages of Nauplius and Copepeditide were 78%, Ciliphora 8%, Sarcomastigophora 4% and Hemichordata 6%. In post-monsoon order of frequency Copepoda 80%, Ciliophora 6%, Coelenterata 5%. Copepoda was the most abundant group of zooplankton.In 2009, The pre-monsoon Copepoda (82%), Cilillophora(9%), Sacromastigophora(4%) and Hemichordata (2%) in post-monsoon Copepoda(70%), Ciliophora (20%),Sarcomastigophora(4%) and Hemichordat (2%) dominante group were respectively. The static result showed significance betweendifferent transects and layers.We were recorded 31 ichthyoplankton families with different density and distribution during 2 years. Among benthic invertebrate polychaete with average 800 ind-2 and then amphipods and gastropods were the most abundance.Yearly density of macrobenthos showed polychaete with the average of 500 ind.-2 in 2007 and 1000 ind.-2 in 2009 was the most abundance . Macrobenthos density in postmonsoon was more than premonsoon.p〈0.05. An increasing and decreasing in density and biodiversity was obtain from coast to depth respectively p〈0.05.There was no significant different between macrobenthos density from Hormoz strait to Golf of Guiter.p〉0.05.There was a negative regression between macrobentos density and depth(p〈0.05) and positive rsgresion between macrobenthos and oxygen concentration and temperature.(p〈0.05).There was also a significant relationship between macrobenthos ,coppepda and polychaete density with organic carbon.p〈0.05.Bimass changed from strait to Golf and coast to depth increasingly.p〈0.05. The results of the measurement of heavy metals in water and Sea bed showed that the Concentration of heavy metals in surface layers in waters of the northern half of Oman Sea and Strait of Hormuz in spring (pre-monsoon) is more than autumn (after monsoon), and Degree of pollution in these areas are low compared to the global scale, Also, The results of the degree of contamination of sediments across the northern half of Oman Sea and comparison Global indicators of environmental quality assessment, it was found that the degree of contamination in the area in spring and autumn are low pollution. Comparison of the TPAH concentration of in Iranian waters of the Gulf of Oman, with other parts of the world indicate that degree of pollution of the waters in the spring 86 in the other seasons too low to medium level is low; but the degree of contamination in sediments, both in spring and in the autumn is much less; It means that the current risk on behalf of these contaminants, does not threaten the fish and benthos. The results of the risk assessment (THQ) of heavy metals in consumer, for yields Sangsar fish and fish Gish showed that the risk for both all the heavy metals, significantly lower than the one(1〈) and This means that urban communities in the province does not present any danger of feeling threatened.
    Keywords: Ecology ; Iran ; Oman Sea ; Strait of Hormuz ; Bay of Chabahar ; Hydrologi ; Sampling ; Chlorophyll-a ; pH ; Nutrient ; Bacillariophyta ; Pyrophyta ; Cyanophyta ; Chropmophyta ; Euglenaphyta ; Silicoflagellate ; Phytoplankton ; Copepeditide ; Ciliphora ; Sarcomastigophora ; Hemichordata ; Copepoda ; Ciliophora ; Coelenterata ; Zooplankton ; Polychaete ; Macrobenthos
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25188 | 18721 | 2018-09-03 17:21:36 | 25188 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Fish sausages were made from minced fish meat and additives using dehydrogenated vegetable oil and fish oil. Four experimental groups were studied; Group 1 with 65% minced fish meat and 12% soybean oil, Group 2 with 65% minced fish meat and 12% olive oil, Group 3 with 65% minced fish meat and a mixture of 6% fish oil and 6% soybean oil, and Group 4 with 65% minced fish meat and 11.3% corn oil and 0.7% cod liver oil. In all the experimental groups the fish was first headed, the entrails removed and then washed and cut into small pieces. The fish pieces were then put in the meat extractor where the skin and bones were separated from the meat. The minced fish meat was rinsed with clean water and the water was let to drain. The additives for each experimental group were then blended with the minced meat and the mixture was filled in the sausage casings. The stuffed sausage casings were then placed in containers and put in a room to cook. After cooking the sausages were packed and stored in the refrigerator. The quality of the product was tested (chemical, microbial and organoleptic) from phase zero through the entire experimental period which lasted 30 days. Based on the results obtained it was evident that sausages in the experimental Group 1 showed better taste and flavor as compared to those in the other groups.
    Keywords: Fisheries ; Iran ; Minced fish meat ; Sausage ; Vegetable oil ; Fish oil ; Additives ; Microbial ; Organoleptic ; Emulsion ; Colour ; Texture
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25189 | 18721 | 2018-09-03 17:16:20 | 25189 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Mariculture is one of the most important sub sector fisheries industry in Asia-pacific region. There are over 40 marine fish species commonly cultured, such as groupers (Epinephlus spp.), snappers (Lutjanus spp.) and Asian sea bass (Lattes calcarifer). But this industry in Southeast Asia experienced serious disease problem since the late 1980s. Khouzestan province has a coastal line about 200 km with many Creek and suitable area for Mariculture. Marine fish culture in Khouzestan province in floating net cages was successfully initiated in Ghazaleh Creek following development of Epinephlus coioides artificial seed production in the 1372. This study has been conducted since 1384 to 1387 in Bandar-e-Imam station. The aim of this research project was to determine the health management status in cage and hatchery, identification of marine fish pathogens (Bacteria, Fungi and parasite), examination of heavy metal in cages sediment and test the physico chemical factors of water in cages and hatchery. Different parts of broodstocks body and fingerlings including intestine, gills and body surface were examined. In this study, 18 Genus and species of bacteria such as Vibrio alginolyticus, Vibrio anguillarum, Vibrio splendidus, Vibrio vulnificus, Plesiomonas shigelloides, Aeromonas hydrophila, Aeromonas caviae, Pseudomonas sp. were diagnosed. Also 7 Genus and species of fungi such as: Aspergillus Niger, Aspergillus flavus, Aspergillus fumigatus, Penicillium sp. And Fusarium sp. Were isolated. In this survey following of disease appearance parasites such as; protozoans Amyloodinium sp. And Trichodina sp. In E.coioides and Acanthopagrus latus and Sparidentex hasta, monogean such as Benedenia sp. In E.coioides and S. hasta, isopoda probably Nerocila sp. In nasal cavity of E.coioides and copepods such as: Caligus sp. And Lernanthropus sp. In A.latus and S.hasta were identified. In this study, microorganisms (bacteria, fungi and parasites) isolated from three different marine fishes species, were reported for the first time in Iran. The range of the physico-chemical parameters of water in cages were: DO (5.42-10.24 ppm), BOD5 (1.51-10.24 ppm), No_2 (0.0197-0.89 ppm), No_3 (3.53-11.93 ppm), Po_4 (0.965-6.05 ppm), turbidity (4-58 NTU) and ammonia (0.0008-0.03 ppm). According to the results parameters such as nitrate, nitrite and turbidity were found more than standard levels. Heavy metals: Ni and Pb relatively high observed.
    Keywords: Biology ; Iran ; Khouzestan province ; Bandar-e-imam ; Mariculture ; Fisheries ; Species ; Epinephlus spp. ; Lutjanus spp. ; Lattes calcarifer ; Bacteria ; Fungi ; Parasite ; Aspergillus niger ; Aspergillus flavus ; Aspergillus fumigatus ; Penicillium sp. ; Fusarium sp. ; Vibrio alginolyticus ; Vibrio anguillarum ; Vibrio splendidus ; Vibrio vulnificus ; Plesiomonas shigelloides ; Aeromonas hydrophila ; Aeromonas caviae ; Pseudomonas sp. ; Grouper ; Snapper ; Sea bass
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25190 | 18721 | 2018-09-04 08:42:55 | 25190 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In recent years MPAs (Marine Protected Areas) have been known as a flexible tool to improve fishery management as well as to preserve biodiversity in valuable coastal waters habitats. This study was conducted to selecting the sensitive and high potential area that are impacted by several environmental stress and associated habitats are being damaged in Mahshahr creeks in Northwest of Persian Gulf. Study was done in eight creeks from October 2005 to September 2006. Different biotic parameters such as phytoplanktons, zooplankton, macro zooplankton, benthic animals and fishes (trawled species) communities and also physico-chemical parameters and sediments heavy metals were investigated. Different parameters were sampled monthly or seasonal from each creek. Water samples with bottle sampler and sediment were collected by Peterson grab. Several ecological and biological indices based on heavy metals and benthic communities were used. Concentrations of heavy metals were measured by using voltammetric plarography method (Methrom 797). The range and mean concentrations obtained in mg/kg were 35.16-15.03 (27.01) for Cu, 171.41-65.57 (102.672) for Ni, 20.06-4.63 (13.22) for Co, 0.78-0.093 (0.22) for Hg, 379-65.07 (113.7) for Zn, 1.00-0.27 (0.559) for Cd and 29.72-7.09 (14.66) for Pb. To evaluate the levels of sediment contaminations, the background values of the different heavy metals were calculated and contamination factor for each metals and degree of contamination for each creek determined as well. Measured concentrations were compared with International standards. Ultimately the heavy metals contamination factors (Cf) can be arranged as follows: Hg〉 Zn〉 Cu≥ Ni〉 Pb≥ Co 〉 Cd and according to contamination degree (Cd), different creeks can be arranged as follows too: Ghannam〉 Ahmady≥ Zangy〉 Doragh≥ Darvish〉 ghazaleh〉 Patil〉 Bihad Regarding the results some elements such as Hg, Zn and Ni, are at risk level and all of the studied creeks are classified in moderate degree of pollution except Ghannam showing considerable degree of pollution. The benthic invertebrates are a well-established target in evaluations of environmental quality status. The AMBI (AZTI,s Marine Biotic Index) was developed to determine the impacts and the quality status in softbottom marine benthic communities. All creeks are characterized by muddy bottom. Macrobenthic animals, according to their sensitivity to an increasing stress gradient, classified in five ecological groups. In present study due to appearance of dominant species such as Capitella sp and nematodes (as opportunist species), diversity values was reduced. Two way ANOVAs showed only seasonal significant differences in mean abundance (P〈0.05, f=5.712, df: 3,21) and Richness index values (p〈0.05, f= 4.975, df=3,21), while all of creeks showed similar biological characters based on benthic communities. According to annual, mean of AMBI (BC) all of creeks classified in ecological group III with slightly pollution, except Darvish that was placed in unpolluted category. In general, according to AMBI and BI values, The most creeks are classified in unpolluted (34%) and slightly pollution (34%) categories except Zangy, Doragh and Patil in summer and also Zangy and Bihad in winter that showed moderate to heavily pollution (32%). The results of chemical quality of sediments, Cf values, Cd values, AMBI Index and water quality and risk Indices have confirmed each other. In general, the Mahshahr creeks are classified in moderate to heavy pollution status. In addition, biological parameters of benthic communities and other biotic parameters have showndescending trend in ecological quality in all of the studied creeks. Communities. According to annual, mean of AMBI (BC) all of creeks classified in ecological group III with slightly pollution, except Darvish that was placed in unpolluted category. In general, according to AMBI and BI values, the most creeks are classified in unpolluted (34%) and slightly pollution (34%) categories except Zangy, Doragh and Patil in summer and also Zangy and Bihad in winter that showed moderate to heavily pollution (32%). The results of chemical quality of sediments, Cf values, Cd values, AMBI Index and water quality and risk Indices have confirmed each other. In general, the Mahshahr creeks are classified in moderate to heavy pollution status. In addition, biological parameters of benthic communities and other biotic parameters have showndescending trend in ecological quality in all of the studied creeks. We used of some criteria for evaluation of biotic potential or sensitivity and also to explain the ecological health level of studied creeks. Positive and negative criteria are classified in three level 1, 3 and 5 for low, moderate and high degree of conservation value respectively. According to gained points the creeks Darvish, Doragh and Ghazaleh in high level and creeks Ghannam , Zangy and ahmady in low level of conservation value were classified.
    Keywords: Biology ; Ecology ; Iran ; Persian Gulf ; Khuzestan Province ; Mahshahr creeks ; Bottom sediments ; Heavy metals ; Contamination factor ; AMBI Index ; Risk Index ; Biodiversity ; Species ; Samples ; Benthic ; ANOVA ; Pollution
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25191 | 18721 | 2018-09-03 17:30:30 | 25191 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The present study was conducted to survey the artificial spawning of European catfish (Silurus glanis L.) by synthetic hormones include Carp pituitary extract (CPE), Ovo fact [D-Ala6 des Gly10] GnRHa Ethyl amid in combination with domperidone diluted in propylene glycol, Human gonadotropin chryonic hormone (HCG) and LHRHa (des-Gly10, [D-Ala6] LH-RH Ethyl amide). In this research work 44 male and 72 female brood stokes that transferred to the Shaheed Dr Ansari hatchery and Sefid rod research station were studied on 2 years and injected with those stimulated maturation. Total length, total weight, gonad weight were measured to the nearest 0.1 cm, 1g and 0.01g respectively. The experiments were conducted using different doses of hormones on 4 treatments. In treatment CPE that brood stocks injected with 2.5, 3, 4 and 5 mg/kg/BW, the most responded (83.33%) was with 5mg/kg/Bw of CPE. The mean fertilization in this treatment was 92.5 4.51 and after that there are groups which were stimulated by 4 mg/kg/BW of CPE .In treatment ovafact that brood fish were injected by 10, 20, 30, 40 μg/kg/BW of this stimulation hormone showed 100 percent of fish ovulated in the group treated with 40 μg/kg/BW of ovofact. The mean fertilization rate was 86.08 6.27 in this group. 33.3 % of brood fish ovulated in the group that injected with 40 μg/kg/BW of LHRHa stimulation, but none of brood stock reached to spawning with injection of 30 μg/kg/BW of LHRHa. The brood stocks were injected by 800, 850, 900, 950 IU/kg/BW of HCG, only 33.3 percent of fish in groups that received 900 & 950 IU/kg/BW of this Stimulation hormone reached to spawning stages. According to our results and statistical analysis between trial treatments in percent of Embryo viability showed significant difference (P〈0.05) between HCG treatment with Ovofact and CPE groups. This results indicates that embryo viability was better in CPE and Ovofact rate to LHRHa and HCG treatments. In the other cases include the working fecundity, fertilization percent, latency period of maturation, latency period of incubation eggs and ratio of weigh stripped egg to body weight did not show any significant difference (P〈0.05) in experimental treatments for stripping the male brood stock of European catfish were injected by 3,4,5 mg/kg/BW of CPE, but not one of male brood fish didn’t reaches to stripping stage , so for eggs fertilization were used of milt from macerated Testes of dead males. So in anther experiment the male brood fish were injected by 5 mg/kg/BW CPE repeatedly at once every 5 days for Period 20 day’s period and milt were collected from of all of the males.
    Keywords: Aquaculture ; Iran ; Silurus glanis ; Artificial propagation ; CPE ; Ova fact ; HCG ; LHRHa ; Spawning ; Hormones ; Carp ; Maturation ; Brood stocks ; Fecundity ; Fertilization ; European Catfish ; Fingerling
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  • 120
    Publication Date: 2021-07-16
    Description: Exploitation of bony fishes resources started on 12 October 2005 and finished on 9 April 2006 in 2005-2006. 142 beach seines caught about 14333.4 tons of bony fishes by 47101 hauling. The total catch was estimated 21844.7 tones (Includes illegal fishery). Kutum, mullets and common carp comprised more than 97 percent of total catch. Length classes 39-40, 31-32 and 40-41 cm predominated for kutum, golden grey mullet and common carp, respectively. Age groups 3 to 5 year comprised 84.2, 74.8 and 83.7 percent for Kutum, golden grey mullet and common carp, respectively and age group 4 year was dominant with 42.0, 35.9 and 43.0 percent, respectively. K value and L_∞ calculated 0.26 (/year) and 58.3 cm for Kutum and 0.15 (/year) and 61.5 cm for golden grey mullet, respectively. The total biomass and MSY estimated about 24733.7 and 8550.4 tones for kutum and about 16948.0 and 4999.0 tones for golden grey mullet, respectively. Exploitation rate (E) calculated 0.71 and 0.70 for Kutum and golden grey mullet, respectively. In 2006-2007, the exploitation of bony fishes resources started on 12 October 2006 and finished on 7 April 2007 in 2006-2007. 134 beach seines caught about 14120.0 tons of bony fishes by 48470 hauling. The total catch was estimated 23801.8 tones (Includes illegal fishery). Kutum, mullets and common carp comprised more than 98.5 percent of total catch. Length classes 39-40 and 38-39 cm predominated for kutum and common carp, respectively and length classes 27-28 and 29-30 cm predominated for golden grey mullet. Age groups 3 to 5 year comprised 80.2 and 71.8 percent for kutum and golden grey mullet, respectively and age groups 4 to 6 year comprised 71.8 percent for common carp. Age group 3, 4 and 5 year was dominant with 35.5, 32.1 and 27.1 percent for kutum, golden grey mullet and common carp respectively. K value and L∞ calculated 0.27 (/year) and 60.7 cm for Kutum, 0.2 (/year) and 58.4 cm for golden grey mullet and 0.19 (/year) and 66.7 cm for common carp, respectively. The total biomass and MSY estimated about 46654.9 and 14801.4 tones for Kutum, about 19549.0 and 5748.4 tones for golden grey mullet and about 10584.3 and 2186.0 tones for common carp, respectively. Exploitation rate (E) calculated 0.69, 0.53 and 0.52 for kutum, golden grey mullet and common carp, respectively.
    Keywords: Fisheries ; Iran ; Caspian Sea ; Bony fishes ; Kuttum ; Golden grey mullet ; Growth and mortality parameters ; Biomass ; MSY ; Common carp ; Coastal waters
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  • 121
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25552 | 18721 | 2018-10-05 17:30:01 | 25552 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Biosecurity means” all activities that decrease the introducing and expanding the pathogen in the shrimp farm” the main activity consisit physical, chemical and biological methods. Some factores has low risck and some of them have high risck level. The level of biosecurity in the shrimp farm and hatcheries is depends the equipmemt and expense that used in the program. For each situation in biosecurity, the scientist will be prepared the standard operation program (SOP) for each risck factor.In the Specific Pathogen Free program that conduted in Bousher, 10 SOP design for different risck factor in the banderga station and Persian Golf Centre for SPF production. The SOP, detecting the main pathogen in shrimp farm in shrimp, feed, water and any equipment in the project. The SOP also consider the disinfectant water with physical and chemival methods such as different net in different size, chemical such as chlorine and ozon and biological filter. During the project studied the methods exclude all pathogen from shrimp and feed and decreased the risck factors in program. The result showed if the culturist used these methods in the production, the safty of shrimp production increased and the sustainable shrimp culture will be available.
    Keywords: Health ; Iran ; Biosecurity ; Ciritical point ; Standard Operation Porogram ; Disaese ; SOP ; Implementation
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  • 122
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25560 | 18721 | 2018-10-05 16:31:39 | 25560 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this investigation, were examined the effect of partial harvesting on optimal management of shrimp culture. In order to achieve to the purpose 6Pool0.4/hectare was selected. Three pool with density Twenty-five per sq. m as a witness and another three pond by density fifty per sq. m as the test sample were storage. Based on the results of biometry and biomass estimates of pool was calculated and provided amounts daily meals for shrimps. The first harvest took place after a period of120 days from the test pools .Average of harvest from pools B4, B6 and B7, was determined respectively, 1373,1365 and 1105kg. Second harvest from treatment poolsB4, B6andB7were determined, respectively, 1663, 1748 and1783kg. Final harvests from the pools were after 150 day. Harvest average from control pools B1, B2 and B3 was calculated and estimated respectively, 1824, 2000 and 1685 kg. The Average of FCR was calculated 1.51 in control samples. In general, the average of feed conversion rate of the treatment samples were calculated 1.51.We did not see the difference between FCR of control and test samples. According to the project's achievements, we can say, partial harvesting has an important role to increased productivity of shrimp farms in Helleh site from Bushehr Province.
    Keywords: Aquaculture ; Iran ; Bushehr Province ; Hellh Site ; Partial Harvesting ; Shrimp Culture ; Feed Conversion Rate ; Penaeusvannamei ; Culture Pond ; Biomass ; Samples
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  • 123
    Publication Date: 2021-07-16
    Description: In conformity with the sex determination of Gracilaria corticata in the Persian Gulf and Oman Sea, a total of 41 samples were collected from two stations of Bostaneh region (northern Persian Gulf, 54° 38´ E / 26° 30´ N) and Lipar regions (northern Oman Sea, 60° 49´ E / 25° 15´ N). The specimens were cultured in PES media for observing the different life stages. The anatomical structures of thallus were taken into consideration. The diploid tetrasporophytes and spermatangia in thallus of male’s gametophytes; and Carpospore and cystocarps of female were determined. Due to DNA extraction, the parasites and epiphytes were cleaned and then the under growing sections were sectioned using liquid nitrogen. After extraction of DNA, by using 20 different primers according to ISSR molecular indicator, the sex diversity and genetic diversity of populations were studied; and four primers were selected ultimately. The obtained results were analyzed by GenAlex and PopGen softwares. In total, 74 bands, all polymorphisms, were propagated. According to PIC index, polymorphism separation of primer C (0.33) was higher than other primers. The Marker Index was measured between 4.48 and 6.51 with mean Shannon’s index of 0.46. The genetic similarity amongst algae was 96%. The genetic diversity inter and intra populations had significant differences of which 83% of total diversity was related to the intra diversity and 17% was related to inter diversity populations. The highest genetic distance belonged to the specimens 5 (Bostaneh) and 35 (Lipar), and it indicated the inter populations diversity in addition to intra population. As an overall conclusion, these populations can be considered as broodstocks for hybrid production for further species breeding and also to attain the maximum heterosis in adaptation with environment. In Ward clustering analysis, the dendrograms showed 5 different clusters in genetic distance of 12.18 of isomorphic phases. The PCA analysis as a complementally method was used for attest the findings. In this research, the ISSR primers could determine the male and female gametophytes and diploid tetrasporophytes in which the primer A (bands of 1200 & 1700 bp) specific for diploid tetrasporophyte and band of 300 bp specific for male were produced. The primer C showed the bands of 820 & 900 bp for diploid tetrasporophyte, and 500 bp for female gametophyte. The primer AB (990 bp) for male, 520 bp for female and 1600 & 1900 bp for diploid tetrasporophyte were specified. The primer ABC showed the specific band of 1100 bp for male; 500 bp for female; and 1200 & 1500 bp for diploid tetrasporophytes.
    Keywords: Aquaculture ; Iran ; Persian Gulf ; Oman Sea ; Bostaneh region ; Lipar regions ; Sexual ; Diversity ; Red algae ; Generation ; Gracilaria corticata ; Samples ; DNA ; Population ; Broodstocks ; Environment ; Gametophyte ; Tetrasporophyte ; ISSR
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  • 124
    Publication Date: 2021-07-16
    Description: Experiments were conducted to evaluate the effects of different dietary levels of protein (%30, %35, %40, %45), fat (%8, %12, %16, %20) and total energy (3500,4000,4500,5000 kcal/kg) on growth of fingerling Roach (Rutilus rutilus caspicus knipowitschi ) in four treatments with triplicates. The crude protein of experiment, fish averaged 2.60 gr stoking density of 25 fish/tank in a during of 57 days, the crude fat of experiment, averaged 5.30 gr stoking density of 15 fish/tank in a during of 67 days and the gross energy of experiment, averaged 5.30 gr stoking density of 25 fish/tank in a during of 57 days which reared into 100L-fiber glass tanks. At the end of each experiments, growth average parameters, such as weight gain(%), specific growth rate, food conversion ratio, condition factor and survival (%) were not significantly different among all treatments (P〉0/05) but protein efficiency ratio showed in fish fed of %30 and %35 protein at levels significantly different with another treatments (P〈0/05). In all of experimentes, significant difference was found whole-body protein, lipid contents of fish fed the different dietary protein, lipid and energy levels indicated that significant difference among of treatments. As regards the economics of feeding and effective protein utilization, the dietary containing %30, %16 fat levels and total energy 5000 kcal/kg would be suitable to growth roach fingerling.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Protein ; Fat ; Energy ; Growgh ; Fish roach ; Rutilus rutilus caspicus knipowitschi ; Fingerlings ; Survival ; Lipid ; Feeding
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  • 125
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25569 | 18721 | 2018-10-06 17:07:25 | 25569 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The Caspian Sea is an important source of water in terms of having valuable resources of sturgeon and bony fish is taken into consideration roach(Rutilus rutilus caspicus (L.)) is one of the most important commercial species in the southern coast of the Caspian Sea in Iran , the catch of this species has declined substantially in the last 10 years, The management and exploitation need of comprehensive review of its biology .This study as part of a comprehensive study of feeding , spawning and growth of this species.In this study, using samples caught in beach seine along the southern coast of the Caspian Sea coastal ( Iranian waters ) took place. The fork length and total weight ranged between 12.5 to29.5(cm) and 29 to293( grm),10.5 to23(cm) and17.2 to21(grm) in Golestan and Gilan Province respectively. The b value of the length-weight relationship ranged 3.02 to 3.25 and 3.28 to 3.75 for female and male, in Golestan and Gilan Province respectively. The age composition of the catch was from 1 to 4 year in both Province, there was one spawning peak and Fecundity variations were high and ranged 7260 to 231965 eggs. Average growth in length was described with the Von Bertalanffy growth model: L (t) = 30.94(1-exp (0.42(t-0.18) and L (t) = 20.49(1-exp (0.53(t-1). The percent of empty stomach and prey dominant evaluated during different seasons by specific formula. Result showed that gastropod, Polychaete worms and molluscs were dominated and specific food items respectively. Shrimp, fish, insects, zooplankton and clams were scare of prey.
    Keywords: Biology ; Iran ; Caspian Sea ; Rutilus rutilus caspicus ; Spawning ; Feeding ; Growth ; Length
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  • 126
    Publication Date: 2021-07-16
    Description: Investigation on small pelagic fishes in the north of the Persian Gulf (Qeshm Island and Bandar Lengeh) coastal waters during October 2006 to September 2008, illustrated that 6 sardine and 2 anchovy species were locally existed. Sind sardinella (Sardinella sindensis) and Buccaneer anchovy (Encrasicholina punctifer) were dominated in this area. The peak of sexual maturity for Sind sardinella and Buccaneer anchovy occurred in spring and summer respectively. Reproduction period for Sind sardinella was from the middle of winter to late spring, and Buccaneer anchovy encompassed reproduction capacity all the year. The female: male ratio was calculated 1:0.7 and 1:0.9 for Sind sardinella, 1:1.27 and 1:1 for Buccaneer anchovy in Qeshm Island and Bandar lengeh sequentially. The mean of absolute fecundity were estimated 16234 and 16168 for Sind sardinella, 1277 and 1141 for Buccaneer anchovy in Qeshm Island and Bandar Lengeh respectively. L_M50 and L_C50 were calculated and the results showed both species mainly caught before length of maturity, and the mass populations have not sufficient opportunity to reproduction. Both species were planktonivore, theirs food prefect mainly consisted of copepods, naplius and bivalves. Vacuity index indicated both spices were semi-voracious appetite fishes. Based on calculated length-weight relationships in S. sindensis and E. punctifer, they have isometric growth. Growth parameters were estimated, the results illustrated that they were growth fast and short live species, which suggested S. sardinella and E. punctifer have 3 and 2 years maximum life span respectively. The recruitment pattern indicated double recruitment peaks per year in both species. Annually, four cohorts were distinguished in Sind sardinella and 3 cohorts in Buccaneer anchovy. Total mortality (Z), natural mortality (M) and fishing mortality (F) were estimated in both species, and the values of exploitation ratio (0.78 & 0.74) in Sind sardinella and (0.72 & 0.41) in Buccaneer anchovy were reasonable for current fishing effort due their short life span. Analysis of the results showed that CPUE in sardine and anchovy affected by reproduction and feeding processes. Results showed significant correlation between sardine CPUE and Sardine GaSI (P〈0.05, r= 0.499), similarly between anchovy CPUE and anchovy GSI (P〈0.05, r=-0.635). Further, significant correlation between sea surface temperature with sardine CPUE (P〈0.05, r=0.493), phytoplankton distribution (P〈0.01, r=0.560), anchovy GSI (P〈0.05, r=0.526), anchovy GaSI (P〈0.05, r=-0.500) and chlorophyll a (P〈0.01, r=0.780). Phytoplankton distribution with sardine GSI (P〈0.05, r=0.453), zooplankton distribution with anchovy GSI (P〈0.05, r=0.671), and chlorophyll with sardine GSI (P〈0.01, r=0.761) have significant correlation.
    Keywords: Fisheries ; Iran ; Persian Gulf ; Qeshm Island ; Bandar Lengeh ; Sardinella sindensis ; Encrasicholina punctifer ; Reproduction ; Feeding ; Growth and mortality ; Catch per unit effort ; Sea surface temperature ; Chlorophyll a ; Sexual maturity ; Plankton ; Naplius ; CPUE ; GSI ; Sardine ; Anchovy ; Coastal waters ; Surface ; Temperature ; Distribution
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  • 127
    Publication Date: 2021-07-16
    Description: Survey is conducted to evaluate the effects of different intensities and durations of red light spectrums on growth performance and survival of Caspian trout (Salmo trutta caspius) in Coldwater Fishes Research Center (CFRC). According to the standard methods and manuals, selected 1.1±0.2 g (1755 individuals) larvae transferred from a registered and well-known hatchery and introduced to the fish tanks in CFRC after quarantine and adaptation procedures. The trial designed for 9 treatments with 3 repeats and fish tanks (California troughs) stocked by 65 larvae. All tanks were separated by dark plastic sheets individually to more controlling actions on special lighting regimes, except controls. Lighting actions were conducted as long as survey stage for 36 days by economic lamps (P: 40/50 W, =550/880 lum) on lighting durations of 12/12, 10/14, 14/10 h (on/off) and 24 (on) by 40/50 W lamps respectively for treatment 1 to 8. Controls are considered in treatment No. 9 by natural and environmental lighting regime. The biometry operations were carried out in a 6 days interval. Results were analyzed with SPSS (Version 12) software and by ANOVA one way analysis method and multiple levels of Duncan Test; graphs were illustrated by Excel software. According to the results, most of the measured factors as growth indicators (FW, WG, FCR and SGR) in treatments (1-8) had showed significant differences in compare of control. In this case, oily drips in retina layer of eye s larvae absorb the short light spectrums and let the long spectrums to be transferred. Thus because of more vision, feeding behavior could develop with lower stress condition. Moreover, some growth factor (SGR, WG and FCR) rates in larvae showed better values significantly in treatment (1) (12/12 h lighting with 550 lum) in compare of other treatments and controls (P≤ 0.05). In addition, there are not any harmonized and expected growth parameters with synchronized red lighting intensities and durations in the Caspian trout larvae. It seems that recent demonstrations were influenced by physiological effects of some endocrinal glands and brain neurotransmitters.
    Keywords: Aquaculture ; Iran ; Caspian trout ; Salmo trutta caspius ; Larvae ; Red light ; Light strengthen ; Light duration ; Growth ; Survey ; Survival ; Coldwater ; Feeding
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  • 128
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25204 | 18721 | 2018-09-04 08:20:25 | 25204 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Rainbow trout is only species of cold water fishes which is rearing and propagation in Iranian fishery farms. Along of develop aquaculture industries increase request of best produces such as trout eyed eggs and larva. For this reason fishery farmers must be to provide that the way of import of other countries, such as: Denmark, Norway and France. As for this importance subject and unknown basic information about preference ratio for doing it, we needed to study about that to answer the suppliant questions. In this study carried out 3 test groups (3 treatments with 3 repetitions) and compared specific growth rate (SGR), condition factor (CF) and survival rate (SVR) between in this groups. Statistical analysis were significantly difference of increase rate of length and weight in French group with other groups (p〈0.05). Compared of main factors (SGR, CF, SVR) in French test group were significantly difference with other groups (p〈0.05). Survival rate percentage in Iranian treatments (SVR=%67) were significantly with the others (p〈0.05). This ratio respectively in Danish and French treatment was %54 and %48.
    Keywords: Aquaculture ; Iran ; Oncorhynchus mykiss ; SR ; CF ; SGR ; Growth ; Survival rate ; Rainbow trout ; Species ; Cold water ; Rearing ; Fishery ; Aquaculture ; Larvae
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  • 129
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25563 | 18721 | 2018-10-05 16:51:28 | 25563 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study, the characteristics of physico-chemical parameters of water done in coastal part of the southern Caspian Sea during 2010-2011 (four seasons) in 8 transects (Astara, Anzali, Sefidrour, Tonkabon, Noshahr, Babolsar, Amirabad and Torkman). For measurement of water quality parameters, 480 samples were collected in different water layers by using of APHA standards methods. According to modeling and statistical methods was used multivariate for evaluation of factor analysis (MFA) in general groups and discriminant analysis (MDA) for spatial and temporal with water quality parameters. The statistical procedure of MFA was used for data reduction and finally six factors selected with about 80.48 percent of total variances related to them. The statistical procedures of MDA were used for the role of spatial and temporal of water quality parameters in different water layer. The function one gave eight parameters (pH, salinity, EC, DO, NO^3-, water temperature, NH^4+ and N^-total) affording more than 80, 67, 80, 77 and 71 % correct assignations (return to the same transects) in spatial analysis. In all transects during four seasons, function one gave five parameters (water temperature, salinity, EC, NO^2- and NH^4+) to afford 100, 100, 100, 97 and 97 % correct assignations in temporal analysis. The results showed that, the average amounts in light penetration layers during four seasons were for EC(15.04±0.18ms/cm), pH(8.38±0.01unit), salinity (10.31±0.16g/l), DO(5.67±0.09ml/l) and water temperature (18.28 ±0.72°C). The average amount of nutrients for parameters such as organic and inorganic phosphorus, total phosphorus, NO^2-, NO^3-, NH^4+, N^-Total and SiO_2 were 0.50±0.02, 0.32±0.01, 0.82±0.02, 1.76±0.10, 0.10±0.01, 1.51±0.10, 46.82±1.80 and 8.60±0.29µM, respectively. The average amounts in dark layers during four seasons were for EC (15.46±0.38ms/cm), pH (8.34±0.03unit), salinity (10.70±0.35g/l), DO (4.55±0.13ml/l) and water temperature (9.62 ±0.16°C). The average amount of nutrients for parameters such as organic and inorganic phosphorus, total phosphorus, NO^2-, NO^3-, NH^4+, N^-Total and SiO_2 were 0.54 ±0.05, 0.33 ±0.02, 0.87±0.06, 1.92±0.21 , 0.08± 0.01, 1.55±0.25, 45.10±3.25 and 10.05±0.62 µM, respectively. The average amounts of light penetration in all transects during four seasons 4.49±0.38 meters. The maximum light penetrations during spring, summer, fall and winter seasons were 9, 8, 8.20 and 7 meters, respectively. The maximum water temperatures in light and dark layers were 32.36 and 11.50°C. Also, the maximum differences about thermocline temperature during summer and fall seasons were 19.6°C and 10°C, respectively in southern part of Caspian Sea. Data in spatial analysis were not significant (p〉0.05), it means if add or removed any transect did not change occurred. But in temporal analysis were significant (p〈0.01), and it means no way remove any seasons for a research work on Caspian Sea in southern part. In those transects with 100m depth. Water temperature is only highly effective parameter between other parameters. In function 1 between 3 functions in transects with 100m depth water temperature with 60% variance was the main role and effective between different seasons in depth part were very high.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Astara ; Anzali ; Sefidrour ; Tonkabon ; Noshahr ; Babolsar ; Physico-chemical parameters ; Nutrient maters ; Sea water ; APHA ; pH ; Salinity ; Temperature ; Nutrients
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  • 130
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25568 | 18721 | 2018-10-06 17:03:55 | 25568 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The beach seines catch and effort data from 1996 to 2011 categorized in four periods and used in this research. Geographical coordinates of beach seines determined by portable GPS and distribution maps of important and commercial species prepared by catch per unit effort (CPUE). The concentration of beach seines in the western coast of Golestan province, Kiashahr area and eastern part of Anzali was more than another part. The daily effort in Kiashahr and Anzali area was very high, thus the fishing stress in these areas was more than another part of Iranian coastal waters of the Caspian Sea. The number of species in catch composition increased from east to west and the beach seines of Guilan province had total of commercial species, but the number of commercial species rarely was five species in Golestan province and especially in eastern coast. Relative frequency and distribution of Kuttum in the east Babolsar area and then between Sepidroud and Anzali lagoon were more than another part. The ISI index of Kuttum was high in all of the area except east coast of Golestan province. The main distribution of Mullets determined in the western coast of Golestan province and the Amirabad fishing area and in these areas, the ISI index of Mullets was high. The main distribution area of common carp determined in east coast of Golestan and the ISI index of common carp was very low in other parts. Density and distribution of Kolmeh were high in eastern and western coasts of Iranian waters of the Caspian Sea and the density of this species was very low in Mazandaran province. The density of Zander was very high in surrounding of Sepidroud River and Anzali lagoon. The ISI index of commercial species in Guilan province was more than other provinces except Kuttum, mullets and common carp.
    Keywords: Biology ; Ecology ; Iran ; Caspian Sea ; Golestan province ; Guilan province ; Mazandaran province ; Babolsar ; Anzali ; Kiashahr ; Bony fishes ; CPUE ; ISI index ; Distribution map ; Species ; Fishing ; Kuttum ; Mullets ; Common carp ; Density
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  • 131
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25200 | 18721 | 2018-09-03 17:38:28 | 25200 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Anzali wetland at south west of Caspian located along of North West and southeast and with its unique feature were add on the Ramsar convention list in 1975. There were recorded a high rich of fauna and flora including 276 plant species, 23 reptiles and amphibians, 60 fishes, 26 mammals and 195 birds. Anzali wetland were encountered with numerous disorder with the eutrophication and the early dismissing. The first studies on Anzali wetland started by many international institutions in 1964. In this study the data of biotic and abiotic factors in Anzali wetland analyzed which belongs to different sites (15 to 38 stations) during 1991 to 2000 and were sampled seasonally or monthly in different years. The results showed the increasing trend of phytoplankton abundance from 7.9 to 66 million C./l. and the western part had the most abundant. The Cyanophyta had the most development than other 7 phylum observed in the area. In Anzali wetland identified 143 phytoplankton genus that 9 genus dominated in more than 80 % replicates and 7 genus had the abundance of more than 5 million cell/l. The Zooplankton abundant increased from 580 to 2200 ind./l. The Rotatoria had the most abundant followed by Copepoda. There was identified 104 zooplankton genus that 5 genus were dominated in more than 80 % replicates and 11 genus had the abundance of more than 30 ind./L. The chlorophyll a varied from 8.8 to 50.2 μg/l. and had a increasing trend after 1996, meanwhile it was measured higher than 25 μg/l in most of the stations. The results of benthic study showed that the Chironomidae and Tubificidae were observed more than other organisms which comprised 12.2 to 23.5% of total biomass of benthic organisms in studied period. The range of biomass was 1.1 to 7.8 g/m2. The ichthyologic survey showed presence of 60 species that 11 species were exotic. 28 species belong to freshwater, 17 species were anadromous and 8 species were resident in estuary. The highest and lowest fish catch were 640 and 288 ton in 1994 and 1999 respectively and it was about 500 ton at the end of period. The hydrochemical factors had the various fluctuation so that the nutrients parameters had a significant difference over studied period. Total nitrogen was varied from 0.78 to 1.41 mg/l and total phosphorus varied from 0.05 to 0.1 μg/l.. The oxygen dissolved amount was from 4.6 to 8.7. EC was between 3 to 5 ms/cm during September and October that indicated of Caspian water projection. According to geographic position of station, all biotic and abiotic data, were arranged. The data bank is used by Idrisi software to produce of necessary maps. Analysis of data showed that there must be a responsible department with a vast authority. Conservation and eutrophication prevention of Anzali wetland connect to special attention to catchment area and landuse management of the wetland. Also there is an urgent need for well-developed technologies and managements to reduce the organic pollution and its environmental impacts on the Anzali Lagoon.
    Keywords: Limnology ; Iran ; Caspian Sea ; Anzali wetland ; Ramsar ; Anzali Lagoon ; Limnological ; Survey ; GIS ; Fauna ; Flora ; Cyanophyta ; Phylum ; Phytoplankton ; Zooplankton ; Chlorophyll a ; Biomass ; Species ; Hydrochemical ; Nutrients ; Dissolved oxygen
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  • 132
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25203 | 18721 | 2018-09-05 16:18:18 | 25203 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Over-fishing of marine resources has endangered many commercial fish species in the world; with aquaculture development, setting up artificial reef systems is an important way for marine stocks and fishing enhancement. The present study was designed to monitor fish abundance and species changes around a small and newly established artificial reef system in Moloo area at Bandar-e-Lengeh during two years after installation (Sep. 2005 to Sep. 2007). The artificial reef includes three types of concrete structures arranged in a seven by three grid. Each cross point considered as a sampling station and two other stations selected from two sides of the system as control stations. Based on obtained results CPUE (P = 0.00001), frequency (P = 0.001) and species diversity of captured fishes (P = 0.024) showed significant differences between three types of sampling traps. With type of structures, The CPUE and frequency of fishes in transect 7(mixed structures) showed the significant differences with other six transects (p= 0.001, P = 0.009). No diversity relationships were seen between transects (p= 0.100). In this study there were no significant differences between depths. Although the means of CPUE between seasons were different, but the ANOVA test could not show the significant differences because of the differention between variances. The T-Test showed no significant differences between the numbers per trap per day dominant species (Epinephelus coioides, Plectorhinchus shotaf, Diagrama pictum, Siganus javus) in different seasons. Movie prepared from artificial reefs showed diversity of fish were more than that of fishing by trap. Although fishes increased but there was no enough causes evidences for product in artificial reefs. Therefore, the study need to continue in this area.
    Keywords: Ecology ; Iran ; Hormuzgan Province ; Bandar Lengeh ; Artificial reef ; Monitoring of stocks ; Density ; Diversity ; CPUE ; Marine ; Species ; Aquaculture ; ANOVA ; Epinephelus coioides ; Plectorhinchus shotaf ; Diagrama pictum ; Siganus javus
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25193 | 18721 | 2018-09-03 17:21:38 | 25193 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Aquaculture for human consuming species is being considered as the first substitution of catching aquatic species due to increase of human population and decrease of wild aquatic stocks. In this study, the hybrid sturgeon Bester (female beluga x male sterlet) was produced for the first time in Iran. Sperm of 1.35 kg male Acipenser ruthenus was used to fertilize the eggs of 125 kg female Huso huso in Shahid Marjani Sturgeon propagation center (Agh Ghala, Golestan province). The fries of bester and control treatment of beluga were transported to International Sturgeon Research Institute (Rasht) after about one month by reaching to 490 mg and 377 mg of weight respectively. All fishes fed by artificial concentrated food (48-50% protein and 15-17% fat) after a period of feeding with Artemia and Daphnia. Sorting was carried out according to increase of fish weight for both fishes. Results showed that the imported sterlet spawners were not at the high maturation stages and especially the males had not suitable sperm quality. It showed that up to 2 months of age , these was no significant difference between bester and beluga weight but from this age up to 2 months of age the weight of beluga was greater. Meanwhile from 2 months old up to the end of the study (21 months) the weight of bester sample was significantly greater than beluga. The comparison of FCR for the whole rearing period showed no difference between bester and beluga (2.4 and 2.3 respectively). In general, the increase and decrease pattern of GR and SGR were coincided to each other, but showed monthly differences. Growth rate (GR) and specific growth rate (SGR) of bester were greater than beluga from 4th and 3rd month of rearing period respectively.
    Keywords: Aquaculture ; Iran ; Golestan Province ; Aquaculture ; Beluga ; Sterlet ; Bester ; Growth Rate ; Aquatic ; Species ; Population ; Female ; Male ; Acipenser ruthenus ; Huso huso ; Sturgeon ; Artemia
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  • 134
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25201 | 18721 | 2018-09-05 16:03:37 | 25201 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Use and enrichment of live food resource in fish farms have been interested and highly demanded. Crustacean are one of the important groups. The Pontogammarus maeoticus dominated in southern Caspian Sea shore with a high abundance. This study was designed in order to adaptation and usage of amphipoda in fish culture ponds. The first part have been surveyed the laboratory experiments including of; to increasing and developing of P.maeoticus in 200 litter container, the effects of salinity on growth and survival of amphipods in many aquariums, the culture of common carp with amphipods and growth determination of them. Chemical composition analysis of P.maeoticus and carps fed by amphipods in compare to cultured carps from ordinary ponds. Due to concern about common healthy the heavy metal concentration has been measured in P. maeoticus, carp which were fed by amphipods and the cultured carps in earth ponds. In second phase; the adaptation of two amphipods species, P. maeoticus and Obesogammarus acuminatus was studied in fish ponds where some cages with sandy soft substrate had been provided for amphipoda replacement. Also a small surface of ponds surrounded by net and covered by Azola plant, a habitat suitable for to putting of O. acuminatus. Production of amphipoda had not the successfully results in large tanks. Aquariums with Caspian Sea water had the prosper results where the specimens were breeding and developing properly, even though in some aquarium with freshwater increased the amphipods number. The chemical composition had not significant difference between two kind of cultured carps while the organic component in amphipoda had a high quality. The better quality of cultured carp by amphipod diet have been confirmed by organoleptic test. The results of heavy metal measurement in amphipoda showed a high concentration which some of them were transmitted to cultured carps. Result of amphipoda replacement in cage was not satisfy and the specimens were died after some days. According to hydro-chemical parameters the oxygen poorness and high trophy levels were the affective factor to abolish of specimens in cages. It seems that there are many type of P.maeoticus that can be adapted in different salinities. The molecular differentiation should be investigated to choose the suitable type of this spices for utilization in freshwater fish ponds. In other hand it can be used in fish culture ponds that will be supplied by brackish water.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Adaptation ; Amphipoda ; Fish ; Culture ; Ponds ; Enrichment ; Pontogammarus maeoticus ; Amphipoda ; Survey ; P.maeoticus ; Common carp ; O. acuminatus ; Oxygen ; Specimens ; Brackish water
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25206 | 18721 | 2018-09-04 08:43:13 | 25206 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: From June 2004 to March 2006, a survey in Iran waters (Bushehr province) has completed. Sampling was accomplished in two area, north and south. In this period 15 cruises achieved in summer and 3 cruises in autumn and winter. In each cruise of north and south area 18 and 30 stations were sampled respectively. Stations row on three depth stratums that is, below 10 m, 10-20 m, and 20-30 m in total area from Bahrekan to Motaf. Some Biological parameters such as sex stages, sex ratio, mean total length, species composition were measured. Duration of shrimp fishing season in years 2004, 2005 and 2006 were 40, 44 and 40 days in summer respectively. In June and august the Catch per Unit Effort (CPUE) in south was more than northern area. CPUE increases in north area especially at the end of summer during fishing season gradually. Computing on Biomass estimation resulted 674, 1024 and 873 ton in years 2004, 2005 and 2006 respectively for all shrimp species (usually tiger shrimp, white shrimp and other). Total commercial landing of shrimp catch in above years were 1602, 1597 and 1110 ton respectively. Percentage of tiger shrimp in commercial landing in above years was 77, 75 and 84 respectively. We found that the least mean length of tiger shrimp was observed in southern area especially from Motaf to Rodmond stations. The schools of prawn penaeus semisulcatus enter to Bushehr waters from southern area that is Motaf region. Other than Motaf; Nakhilu, Rase khan and Rod-mond show same characteristics. Also in this region, density of shrimp increases with depth in most summer cruises. The result from analyses of gonad maturation studies shows the adult and mature females (stages III and IV) of P. semisulcatus accumulate in northern area at the end of autumn. This stock catches in autumn by illegal fishing.
    Keywords: Fisheries ; Iran ; Persian Gulf ; Bushehr province ; Biomass ; Shrimp ; Penaeus semisulcatus ; Fishery ; Green tiger prawn ; Opening season ; Closure season ; Survey ; Sampling ; Sex ratio ; Species ; CPUE ; Illegal fishing ; Parameters
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  • 136
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25208 | 18721 | 2018-09-05 16:18:49 | 25208 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: To introduce the native commercial shrimp species to aquaculture industry, the Iranian Fisheries Research Organization conducted a project to investigate the possibility of reproduction and cultivation of the banana shrimp (Penaeus merguensis). The present work has been carried out from 21st March 2001 in two phase, reproduction and cultivation, in Iran Shrimp Research Center. The main objectives of this study was to find the possibility of reproduction and cultivation of banana shrimp with emphasis on its cost and benefit and to find if this species could be introduced to aquaculture industry in Iran. In this work, 42 brood stocks of banana shrimp caught in Jask waters and were transported to Bushehr. The shrimps were reproduced in aquaculture research station, and then cultivated in 0.4 ha ponds in Heleh region. Most of the broodstocks perished, due to long distance of Jask from Bushehr province, or had problem in hatching. Finally 8 of broodstock shrimps spawned. In 3 cases, the reared naplii larva suffered high mortality, but the other 5 spawner’s bread successfully. The post larvae were introduced to the shrimp ponds. The primary stocking was 360,000 larvae, introduced to each of two 4 m 3 tanks. The survival in both tanks for mysis stage was 83% but for PL stages, the survival was 5% for Tank 1 due to disease outbreak (until PL 24), and 62% for Tank 2. On 10 th June 2001 the PL 24 were stocked into the ponds but unfortunately due to adaptation problem, all of the shrimps perished. It seems that banana shrimp couldn’t to be adapted to the pond condition in Heleh region. On the basis of the results, the reproduction of the banana shrimps is achievable, but broodstocks needs to be transferred to reproduction areas as soon as possible to reduce stress on the shrimps. Our finding shows that the optimum conditions of salinity and temperature for growth of the reared banana shrimp in Heleh region is 33ppt and 30 °C, respectively.
    Keywords: Aquaculture ; Iran ; Persian Gulf ; Banana shrimp ; Aquaculture ; Reproduction ; Penaeus merguensis
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25207 | 18721 | 2018-09-05 16:22:47 | 25207 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Increasing of biodiversity and reduction of production costs and alo increasing of shrimp production are the main elements of production security and continuation of aquaculture of shrimp in the country. The hypothesis of this study is made on the basis of aquaculture of pacific white legged prawn, litopenaeus vannamei, and that is economically better than Indian white prawn in Bushehr area. This study was carried out with the objectives of determination of bionormatives of pacific white legget prawn and yield estimation per square meter and has been compared with Indian white prawn. During 1384-85. In this study, the field operations were carried out after primary preparation and water enrichment with the juvenile prawn (pL 15) with 20 prawn per squar meter in the 3 ponds. During the study the phisico-chemical parameters such as water temperature, ocsysion, pH,two times per day , in the morning and evening and salinity , transpiration and depth water , only one time in a day were measured .The health of prawns and also sampling for the calculation of body weight were carried out in every 10 days . Feeding in the first month was randomly and after that was being continued based on the mean of the body weight. The results of this study have showed that the growth of this species is very fast so that during 90 days with the capacity of 20 prawns per squar , meter, it can be reached to 19.59 gr body weight with the growth rate of 0.217 gr . The mean of total production was 2737.5 kg per hectar with FCR=1. During this study the salinity from 33 ppt to 54 ppt, water temperature from 24.3°c to 35.2°c, occsysion from 2.4 to 11.89 mg/l , pH from 7.83 to 9.01 , transparency from 35 cm to 90 cm and water depth from 100cm to 145 cm were measured and recorded . In the second year, the comparison of growth rate of white legged prawn and Indian white prawn was carried out in the four ponds. The results of this study showed that during 110 days. White legged prawns was reached to average of body weight 14.66 gr and Indian white shrimp, reached to 10.75 gr. The economical assessment showed that white legged prawn has an economical advantage around 2.59 times during 110 days with the capacity of 35 prawns per hectar.
    Keywords: Aquaculture ; Iran ; Bushehr ; Western white legged shrimp ; Indian white shrimp ; Culture ; Bionormative ; Commercial production ; Aquaculture
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25215 | 18721 | 2018-09-06 05:02:27 | 25215 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was done is two phases, to determine required suitable level of protein and energy for Barbus grypus to get maximum growth to market size. Phase 1-finger ling phase Treatments with triplicate in different level of protein (25, 30, 35 percentage) and different level of digestible energy (kcal/100 gr 250,300,350) were used. Also the effect of mentioned diets on fish growth by calculating (WG, FER, FCR, SVR, PER, SGR) was studied. 9 treatments with triplicate random group of 15 fish per 300 liter tank the mean of were stuclied for 60 days. Body weight was (29.68±0.19gr). The results showed that in constant protein levels growth parameters improved to a special level with in Creasing energy level but in constant. Protein decrease with in crase in energy level. The result of caracass showed energy, protein, fiber and lipid caracass increased with increase in protein and energy, ash level of diet, on the other hand the mentioned diets had meaningfull effect on biochemical composition of caracass except (protein and ash). The best protein level was (%30) and suitable energy level was (250 Kcal/100gr). Phase 2: Grow out stage The best diets (P30%) and (250Kcal/100gr) and (p350%),(300kcal/100gr),(p35%)(350Kcal/100gr)was determind of second phase. These three suitable diets 3 treament in a soil pond 160m² with primary weight 98.3±23 in 60 day. Siqnificant different bet ween treatments were obsereved based on WG,FER,FCR,SVR,PER,SGR Indices. There were no Siqnificant different in (fish body composition)in second phases of experiment. The level of energy,protein,fiber and lipid. In fishes body were increased with in increasing the diet protein. Finally, according to mean valves,30% protein level and (250 Kcal/100gr) energy for barbus grypus are suggestedias the best levels.
    Keywords: Aquaculture ; Iran ; Barbus Grypus ; Protein ; Energy ; Fingerling ; Grow out stage ; Diet ; Body weight ; Ash ; Parameters ; Growth
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25217 | 18721 | 2018-09-06 06:02:14 | 25217 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: A variety of products made from fish meat is increasingly being produced in most countries. The availability of low cost ingredients, the use of suitable formulations and modern equipment has made it feasible to produce different products of high nutritious value to satisfy consumer demands. Starch is an ingredient used in most products in the food industry because of its physical characteristics. In the present study fish ball were prepared using starch from two different sources (wheat and potato) either individually or by mixing equal proportions of two sources of starch at a rate of 5, 10 and 15% to 80% ground meat of silver carp. A control group was also tested without adding starch. Results obtained indicate that flavor, odor and texture of the resulting product made with 10% starch were more acceptable as compared to the other trials. Fish ball made with the addition of 15% starch were lighter in color and sticky and loses its strength to form. No significant differences were observed in color, flavor, odor and texture of fish ball in trials containing 10% starch from one individual source (p〉0.05) and the resulting product in these trials were rated higher in organoleptic tests as compared to those containing 5 and 15% starch. Among trials containing two sources of starch, mixed trials with potato and wheat (5% potato + 5% wheat) were rated higher in organoleptic tests as compared to the other trials(p〈0.05).
    Keywords: Fisheries ; Iran ; Silver Carp ; Fish Ball ; Starch ; Sensory quality ; Nutritious ; Hypophthalmichthys Molitrix
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    Publication Date: 2021-07-16
    Description: In this study population genetic structure of Mnemiopsis leidyi were examined by 200 samples from the Caspian Sea. Samples were collected from Guilan province (Astara region), Mazandaran province (Amir Abad region), Golestan province (Gomishan region) and north part of the Caspian Sea. 50 samples were collected from each region. DNA was extracted by phenol - chlorophorm method and its concentration was 50 to 100 nanogram. PCR was performed using 18s rRNA gene. The PCR products of samples were digested by 12 restriction endonuclease enzymes. The digested products accompanied with standard marker (50 bp lader ). In order to measure fragment size samples were run on a 6% vertical polyacrylamide gel. The fragments were visualized by silver staining of the polyacrylamide gel. In this study, Dra I and Alu I enzymes showed different digestion pattern, as each of these enzymes had two genotypes. Other enzymes had similar digestion pattern. RAPD method used by 19 random primers. Ten of nineteen primers showed polymorphism patterns. Statistical analysis of data was performed by Popgene software. In this study, the maximum of genetic variation was in north of the Caspian Sea. Also, the maximum of genetic distance was between north area and Golestan coasts (0.089) and the minimum of genetic distance was between Mazandaran and Guilan coasts (0.001). The UOGMA dendogram showed two clusters. The samples of Mazandaran , Guilan and Golestan coasts placed in one cluster and samples of north area in other cluster The genetic diversity was significantly different between samples of north area and Golestan coasts (p≤0.05). As result, there is a significant genetic divergence between some of samples. Therefore, two genetic group of Mnemiopsis leidyi were identified in the Caspian Sea.
    Keywords: Biology ; Iran ; Caspian Sea ; Guilan province ; Mazandaran province ; Golestan province ; RFLP ; RAPD ; Mnemiopsis leidyi ; Genetic variation ; Population ; Molecular Test ; Samples ; Enzymes ; Genotype
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25615 | 18721 | 2018-10-07 16:19:08 | 25615 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Aras dam reservoir situated in the northwest of Iran, west Azarbaijan province, is the only water resource of Astacus leptodactylus harvest in the country that more than 250tons of this species were exported to different countries all over the world, annually. On the other hand, one of the polices of Iranian Science Fisheries Institute is the release of this species into other water resources in the country and for this purpose, the study of risky diseases such as Crayfish pest (Aphanomysis astasi) and other zoonotic diseases are considered as the research priorities of aquaculture development of the country. This study was carried out to health screening of Astacus leptodactylus at Aras dam reservoir from winter 2013 to fall 2014. In this regard, A total of 394 harvested livefreshwater crayfish Astacus leptodactylus (255males, 139females) weretested. 9 epibionts and parasites peritrich protozoans were identified. From Metazoan parasites group, Branchiobdella kozarovi with incidence rate of (100%) in obtained samples was the only isolated organism from this group that identified up to species level. There was a heavy damage in gills of samples with Aeolosoma hemprichi (Annelid) in winter with90% prevalence. Furthermore, Other Epibiont fouling organisms such as Rotatoria; free living nematods and suctoria were observed in this survey. The fungi study of the lesions and melanized spots of mentioned samples revealed their infection to Penicillium expansum; Aspergillus flavus; Alternaria sp. ; Fusarium sp. and Saprolegnia sp. The results of bacterial study confirmed the presence of pathogen bacteria in Astacus leptodactylus. The most frequency percentage (15.16%) in hepatopancrease were related to Aeromonas hydrophila and the least one (1.37%) were due to Yersinia bacteria. Also, only Aeromonas hydrophila and Staphylococcus aureus were isolated and identified from heamolymph, respectively. The results revealed that the combination of Salmonella typhi, Escherichia coli and Staphylococcus sp. has caused the most infection rate while. Yersinia ruckeri and Salmonella typhi has caused the least infections in Astacus leptodactylus. According to the isolation of 6 bacteria species from hepatopancreas and 2 species from heamplymph , it can be concluded that hepatopancreas enjoyed the higher infection rate compared to haemolymph in the obtained samples.
    Keywords: Ecology ; Iran ; Aras Dam Reservoir ; West Azarbaijan province ; Astacus leptodactylus ; Health screening ; Hygienic ; Freshwater ; Crayfish ; Species ; Fisheries ; Aphanomysis astasi ; Parasites ; Metazoa ; Aeolosoma hemprichi ; Epibiont fouling ; Alternaria sp.
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25607 | 18721 | 2018-10-07 16:14:00 | 25607 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Acipenser stellatus and Huso huso are the most important native sturgeon species in Caspian Sea. In the present study, the impacts of various concentrations of Aflatoxin B1 (AFB1) on Beluga, Huso huso, under controlled conditions were investigated. Belugas (120±10 g) were fed diets containing 0, 25, 50, 75, and 100 ppb AFB1/kg of diet for 3 months. Results showed that various levels of AFB1 do not significantly affect the specific growth ratio (SGR) (p〈 0.05) of fish in different treatments. However, weight gain and food conversion ratio (FCR) varied significantly (p〈 0.05 between control and treatments with diets contaminated with 75 and 100ppb AFB1/kg after 90 days). The increase in level of AFB1 did not affect the percent of survival rate (SR) and no mortality was observed in treatments (SR=100%). Various levels of AFB1 under experimental conditions of the present study affect some growth factors, such as, weight gain and FCR but have no significant impact on SGR. Histopathological studies showed that different level of AFB1 can cause broad range of changes in liver, kidney, spleen and gills tissues, particularly at concentration of 75 and 100 ppb AFB1/kg of diets after 60 days.No tumour formation observed. With regard to toxin concentration and time of exposure to AFB1 in experimental fish, different degree of skin lesions (simple hemorrhage to progressive wounds) were observed in different parts of body especially in vent, caudal peduncle, fins, and head. "Yellow sores" on head and trunk regions are considerable and led to deterioration of appearance. Prevalence of skin lesion in different treatments was 8 -53.3 %, which after stop feeding with toxic diets, 16 – 24 % healing observed.Haematological chenges included chronic anemia and lymphocytopenia.Also nutrophilia observed with increasing of skin lesions. Meat accumulation of AFB1 in different treatments is not so considerable and harmfull for human cunsumption, but is significantly different with control fishes(P〈0.01) Responses of experimental fishes to varying concentrations of aflatonix were investigated under controlled conditions. Fishes were fed diet containing 0, 25, 50, 75 and 100ppb AFB1/kg of diet for 3 months. Skin lesions evaluated through clinical observations. With regard to toxin concentration and time of exposure to AFB1 in experimental fish, different degree of skin lesions were observed in different parts of body. Histopathological changes in liver, kidney, spleen and gills of fish fed different level AFB1 and very wide range from congestion to necrosis. Progressive fat deposition, hepatocyte degeneration and necrosis in liver, especially in concentration 75 and 100 AFB1/kg of diets after 4o days of feeding, emphasize the mentioned results .At the end of the 30 days exposure period , blood samples were taken from the control and experimental fish. Blood was assayed for selected haematological parameters (haematocrit, hemoglobin, red blood cell count, white blood cell count, total albumin, total albumin, ALT and AST).The derived haematological indices of MCH, MCVand MCHC were calculated. Alterations in serum levels of ALT and AST are liver specified and the significant reduction in total protein and albumin content observed in some experiments.There was no significant difference in SGR between experimental treatments but some differences observed in GR and FCR.
    Keywords: Aquaculture ; Health ; Iran ; Huso huso ; AFB1 ; Growth ; Skin lesions ; Pathological changes ; Haematological changes ; Meat residue ; Acipenser stellatus ; Histopathological changes ; SGR ; FCR ; Aflatoxin ; Beluga
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  • 143
    Publication Date: 2021-07-16
    Description: After the arrival of the invasive spexies (Mnemiopsis leidyi) in the ecosystem of Caspian Sea in 1999, It had notable impacts. In this study, we,re assessing impacts of quantitative M. leidyi as a biopllutant on zooplankton community in the southern part of the Caspian Sea during 2001-2010 period. It becomes clear that Abundance and Distribution Range (ADR) of M.leidyi during 2001-2009 was in class E(occurrence in high numbers in all localities) and in year 2010 was in class D(occurrence in moderate numbers in all localities). from 2001- 2009, ADR in Summer season was in class E, but in summer 2010, relatinve biomass of M.leidyi has decreased and ADR was evaluated as D. During the fall seasons of 2001 -2010, only in fall of 2003 and 2010, the ADR was C(occurrence in low numbers in all localities) and D respectively and in other years, the ADR was in class E. In winter season relative biomass of M.leidyi decreased and only in winter of 2001 relative biomass was high and the highest ADR (class E). In winter 2003 and 2004, ADR was in class D. The biomass of M.leidyi was found to be zero in winter 2008. From winter of 2009-2010,ADR, were assessed B and A respectively. In the spring, ADR was evaluated A and D. Considering the obtained results, the yesr 2001, which the relative niomass was more than 90 percent, could be considered as the expantion phase and the following years (2002-2010) with regard to decrease of relative biomass as the adjustment phase. With regards to the loss of the keystone species (Eurytemora sP.) and some other native species, our evalution showed that the impact of M.leidyi on structure of zooplankton community was assessed as massive (C4).During summer and fall seasons that biomass of M.leidyi was higher than 50%, only A.tonsa exist, but during spring and winter seasons that biomass of M.leidyi was decreased and more species have a chance to exist.
    Keywords: Ecology ; Iran ; Caspian Sea ; S Caspian Sea ; Mnemiopsid leidyi ; Zooplankton Community ; Native species ; Bioinvasions
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25618 | 18721 | 2018-10-07 16:34:53 | 25618 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Chitin and chitosan are 2 very important products of biopolymer that enjoy high consumption in industry, but their production sources are very limited. In this study, Artemia urmiana cyst shells were obtained from previously collected and stored ones in Iranian Artemia Research Center. 20 kg of Artemia urmiana cyst shells were sampled, cleaned, separated, dried and transferred to Iranian Artemia Research Center Laboratory to extract their Chitin and chitosan. Their chitin and chitosan initially were extracted using optimized common chemical methods. Their properties were compared to 2 other types of Chitin and chitosan obtained from crab and shrimp manufactured by Vietnam and China, respectively. To determine their quality, elemental analysis device, infrared spectrophotometry, x –ray radiography, determination of viscosity , molecular weight, crystallinity percent, color, de stylization measure, empirical and molecular formulas were made. The results showed that the percentage of chitin obtained from Artemia cyst Shells in Chemical method was 28 ± 3 % by weight and efficiency into chitosan (grade steel relief) in this method was 50± 5%. To optimize the extraction procedure and the removal of proteins of chitin by biological practices that were done by sodium hydroxide in the chemical method, it was replaced by the bacterium Bacillus subtilis. And in the bio- phase of chitosan de steelation fungus Aspergillus niger enzyme was replaced instead of sodium hydroxide at high temperatures. The results showed that chitin and chitosan can be extracted from Artemia cyst shell using biological method and their characteristics included as in chitin 49.6% C, 8.2 % N, 7.5 % H, and 34.5 %O. Also the same levels for chitosan were 44.4 %, 8.9, 7.2 and 39.5 %, respectively. Their other quality characteristics were included chitin average molecular weight 4.9×10^6 Dalton, crystallinity percentage of 36.4, viscosity at 20°C 31 centipoise and its color was gray to brown. In the biologic method, the average molecular weight of chitosan, crystallinity percentage, viscosity at 20°C, were 5.1×105 Dalton, 94.5, and 18 centipoises, respectively. Also, its color was pale brown. Chemical structure of extracted chitin and chitosan from the shell of Artemia urmiana cysts were C_7H_12NO_4 and C_6H_11 NO_4c, respectively. The comparison of chitin and chitosan obtained from each chemical and biological method revealed that replacing biological methods instead of chemical methods is possible in achieving these products at suitable condition and better quality. This can eliminate the use of chemicals damaging the environment such as sodium hydroxide and decrease environmental pollution.
    Keywords: Biology ; Chemistry ; Iran ; Artemia urmiana cyst shell ; Chitin ; Chitosan ; Chemical ; Biological methods ; Crab shell ; Shrimp shell ; Biotechnology
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    Publication Date: 2021-07-16
    Description: Barcodes are short segments of DNA that can be used to uniquely identify an unknown specimen to species, particularly when diagnostic morphological features are absent. These sequences could offer a new forensic tool in plant and animal Conservation-especially for endangered species. It was proved that a small fragment of mitochondrial DNA from the 5′-end of cytochrome c oxidase subunit 1 (COI) gene as a reliable, quick and costeffective identification system for most Crustacea like shrimp. Take a look in DNA barcoding website show that there is a little data about Iranian shrimps which live in Persian Gulf and Oman Sea. In this Study six species of shrimp: Fenoro penaeus indicus, Fenoro penaeus merguensis, penaeus semisulcatus, Metapenaeus affinis, Marsupenaeus japonicas, Fenoro penaeus penicillatus were collected from different stations in Persian Gulf and Oman Sea. All materials were preserved in 70% ethanol and were shipped to the laboratory for taxonomic studies. After identification, the total DNA was extracted; COI gene was first amplified and then sequenced for each species. Finally the collected data were analyzed with the specific phylogenetic software. The results were amazing and the interesting part was that analytical methods for showing species relationship suggested that Fenoro penaeus penicillatus is closer to Marsupenaeus gnus than penaeus gnus. This finding needs more investigation to be proved. We suggest a workflow for DNA barcoding, including database generation and management, which will ultimately be necessary if we are to succeed to join universal DNA barcode for Crustacea.
    Keywords: Biology ; Iran ; Persian Gulf ; Oman Sea ; DNA ; DNA barcoding ; Species ; mtDNA ; CO1 ; Molecular method ; PCR ; Specimens ; Morphological ; Cytochrome ; Mitochondrial DNA ; Shrimp ; Fenoro penaeus indicus ; Fenoro penaeus merguensis ; Penaeus Semisulcatus ; Metapenaeus affinis ; Marsupenaeus japonicas ; Fenoro penaeus penicillatus
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25612 | 18721 | 2018-10-07 16:15:05 | 25612 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Kutum fish (Rutilus kutum) is an anadromous cyprinid species that spawns in adjusted rivers to the Caspian Sea. Many million fingerlings were artificially produced and annually released in these rivers specifically in Sefidrud River by Iranian Fisheries Organization. In this study were estimated the staying period of Kutum fingerlings in Sefidrud River and its diet and growth. A small piece of the caudal fin was cut as a tagging method. About 50000 and 5200 individuals were marked during July and September 2013, respectively. The marked fish had an average total length 53.2±5.6 mm and body weight 1.11±0.26 g and were released in river around two kilometers of the estuary. The sampling of fishes had been earlier started from Feb. 2012 and continued until Feb. 2013 at five stations. Four stations were along river from three Kilometer of estuary to estuary and one station was in right side of estuary in the sea coast. The results showed that marked samples after releasing time were distributed in all studied area from 3 kilometers of estuary to estuary but they migrate mainly toward Caspian Sea in a short time. However the marked samples were observed in estuary and the sea coast after 6 and 24 hours of their releasing time, respectively, Kutum fingerlings remained in Sefidrud river ecosystem in a longer time, at least until end of our sampling period i.e. 105 days. The coefficient of vacuity index of the natural feeding study was 0.0, 49.0 and 30.6 % for phytoplankton, zooplankton and benthos, respectively. The average of GastroSomatic index, intensity of fullness and condition factor were calculated 6.30±3.25, 174.6±153.2 and 0.92±0.09, respectively. A temporal and spatial variation on main food items were observed in gut content of fish fingerlings from different locations and seasons. Gut contents were generally included 59 genera of phytoplankton, 15 groups of zooplankton and 10 groups of benthos. Nitzschia, Navicula and Synedra were dominated among phytoplankton genera with 45.83, 18.02 and 15.99 %, respectively. Among zooplankton groups; the Rotaria, Moeina and Difflugia were dominated with 22.30, 20.86 and 15.83%, respectively. Chironomidae and Gammaridae were the main food items among fed benthic animals with 83.67 and 11.15%, respectively. Furthermore 48 phytoplankton genera, 32 zooplankton genera and 6 benthic families were identified in ecosystem of Sefidrud river. Bacillariophyta phylum (with 23 genus), Rotatoria (with17 genus) and Chironomidae family were dominated in each mentioned groups, respectively. It is concluded that the Kutum fingerlings stay in Sefidrud River for a long time, although most of them migrate to Caspian Sea during first month after releasing. However dietary indices were normal in studied samples, the condition factor index seems to be low.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Rutilus kutum ; Fingerlings ; Kutum ; Fish ; Rotaria ; Moeina ; Difflugia ; Phytoplankton
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25623 | 18721 | 2018-10-08 07:07:34 | 25623 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The conservation of coral colonies is very vital for marine organisms and human. In Chabahar Bay, the coral reefs are in danger of destruction due to the development program of Shahid Beheshti Port. In this project, over 30,000 hard corals were transported to coast of Hotel Lipar an area at a distance of 3.5-4 kms far from Shahid Beheshti Port. Also, the new techniques were used for coral reattachment and transportation. The operation has done within expert divers and consultancy of Prof. Dr. Maikle Risk. Four methods performance as pailots. Since the corals are very sensitive to turbidity and suspended sediments from land reclamation and dredging projects, therefore appropriate measures should be conducted for conservation and recovery of them.37 patches in size 5×10 meters has built with concerete. Diffent sizes of corals were separated by mechanical tools and transported to new sites and reattachment on artificial sea bottom.Three monthes after the last reattachments monitored showed that %96.93 of corals were in good health. This success was the important achievement because of innovating coral carrier and mass coral relocation. At present, the coral relocation is suggested as a good method for recovery of coral reefs after a disturbance in condition of their native habitats.
    Keywords: Aquaculture ; Iran ; Chabahar ; Port of Shahid Beheshti ; Corals ; Coral relocation ; Reattachment of Corals ; Marine ; Organisms ; Reefs ; Turbidity ; Sediments
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25617 | 18721 | 2018-10-07 16:24:05 | 25617 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The project is funded by the CASPECO Project which is the Third Phase of the GEF supported project in the Caspian Sea region titled “The Caspian Sea: Restoring Depleted Fisheries and Consolidation of a Permanent Regional Environmental Governance Framework”, and implemented by the Iranian Fisheries Research Organization (IFRO) in partnership with four Coastal Research Centers affiliated to IFRO, Coldwater Fishes Research Centre (CFRC).The project's objective is to improve the conservation and restoration of Caspian Salmon (Salmo trutta caspius) stocks. In accordance with IUCN criteria Caspian Salmon is placed in the red list of endangered fish. In the recent decades the destruction of habitats for juveniles, reduction of genetic diversity and fish immune system were main causes of the Caspian salmon decline. Numbers of public meetings were held with participation of fishing communities’ representatives, experts, relevant local authorities and experts of the IFRO and Iranian Fisheries Organization (IFO), Iran Environment Protection Organization (IEPO), Water Resource Management (Ministry of Energy) and University senior professors, in order to create interaction and understanding of the project objectives. Educational awareness and extension program were conducted with the involvement of fisheries communities, private sector and NGO associations. The results of the project suggests that cross-sectoral coordination for sustainable fisheries can be achieved through building partnerships and enhancing communication between the environment, private, fisheries sectors and academia. Cross-sectoral cooperation through this project improved public awareness on growing risks facing Caspian Salmon from different sources including local sources (illegal fishing and over-fishing, urban wastewater, environment destruction, industrial pollutants, pollution of air and water resources), new emerging and invasive organisms, climate change, implications of the Caspian salmon population decline on economic and social affairs of the people who depend on them for their livelihood and food security and their role in fish stock protection. The project, through its capacity building, extension and educational programs and efforts to link science to management, could translate scientific findings into an action agenda for managers and policy makers that can bring an improvement in the future of the Caspian Sea salmon stock.
    Keywords: Management ; Iran ; Caspian Sea ; Conservation ; Salmo trutta caspius ; Caspian salmon ; Fisheries ; IFRO ; Juvenile ; Genetic diversity ; Immune system ; Illegal fishing ; Pollution
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  • 149
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25622 | 18721 | 2018-10-08 05:50:41 | 25622 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The overfishing and changing environmental following the introduction of the comb jelly Mnemiopsis leidyi in the Caspian Sea, the population structure has a strong influence on kilka fishes in the Caspian Sea and therefore to in this investigation which had been done in commercial catch regions (where discharged theirs catch) in three ports Babolsar, Amirabad (in mazandaran) and Anzali (in Guilan), stimated catch and catch per unit effort, the age structur of catch, length- weight relationship, von Bertalanffy growth parameters, condition factor, sex ratios, maturity stags, spawning and feeding. The result shown that catch of kilka in Iranian coastal in 2012 decreased from 24080 ton to 22696 ton in 2013. But CPUE increased was 2.7 ton (Vessel ×Night) and there was no significant changes. Common kilka was the predominant all months of the year, 98.1 and 98.9 percent in 2012 and 2013 respectively. The mean length was 104.4±10.5 and 101.6±11.2 at the same time and range length 92/ 5 – 117/5 mm were the dominant population(about 75%). Relative abundance of fish less than 77.5 mm and more than 127.5 mm is very low. Age abundance during this period have not been substantially changed and always fishes with 3 and 4 year olds had the highest frequency73.6 and 73.5 percent respectively. The parameters of the Von Bertalanffy growth curve were (8.141) 048.1 for common kilka. The instantaneous coefficient of natural mortality (M) was 0.506 yr-1. The instantaneous coefficient of fihing mortality and total mortality were 0.694 yr-1 and 1.2 yr^-1 , respectively. The exploitation rate of common kilka varied during 2012-2013 between 0.41-0.50. The mean condition factor 1.41±0.14 (n=5801) and 1.38±0.16 (n=6754) at the same time respectively. According to statistical analysis Anova oneway were a significant difference between condition factor at the same time . Main prey common kilka was Acartia tonsa accounting for over 80%. It seems Common kilka due to a decrease in the frequency of other zooplankton species depend more than ever on Acartia tonsa and this species is probably a concentration of prey for Common kilka.
    Keywords: Biology ; Fisheries ; Iran ; Caspian Sea ; Mazandaran Province ; Guilan Province ; Anzali ; Babolsar ; Kilka fishes ; Age ; Growth parameters ; Condition factor ; Exploitation rate ; Feeding ; Reproduction ; Overfishing ; Comb jelly ; Mnemiopsis leidyi ; Population ; Sex ratios ; Maturity ; Spawning ; Mortality ; ANOVA ; Acartia tonsa
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25230 | 18721 | 2018-09-06 06:05:40 | 25230 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The ecological situation of Maharloo Lake is of a great importance in the province, especially in relation with continuous addition of a reasonable volume of municipal and industrial effluents to it; one major point being a source for different life cycle stages of the crustacean Artemia, which is used in aquaculture as natural food. In order to improve our knowledge about lake's pollution, we investigate mineral nutrients and heavy metal concentration, among some other physical and chemical characteristics. Three stations were selected in the lake, sampling trips were made, and the concentration of selected factors were measured monthly from January 2000 to December 2001. Using ANOVA, results were used to compare means of monthly factors between three stations, and means of three stations between 12 months. The yearly means for pH, dissolved oxygen, salinity, electrical conductivity, water temperature, nitrite, nitrate, free ammonia, carbonate, bicarbonate, dissolved CO_2, phosphate, alkalinity, chloride, calcium, magnesium, total hardness, total dissolved solids, and total particulate matter were 7.95 (SD=0.558), 2.92ppm (SD=2.11), 290.73ppt (SD=168.08), 375.52ms/cm (SD=232.72), 20.8 C (SD=7.4), 0.051ppm (SD=0.058), 0.111ppm (SD=0.089), 3.342ppm (SD=2.894), 67.75ppm (SD=66.84), 270.88ppm (SD=190.91), 63.57ppm (SD=37.94), 0.018ppm (SD=0.026), 328.97ppm (SD=216.14), 128.05ppt (SD=76.65), 3334.90ppm (SD=4769.87), 22719.22ppm (SD=45204.82), 18059.73ppm (SD=12513.54), 203.20ppt (SD=132.40), and 8.65ppm (SD=5.49), respectively. Heavy metals' highest concentrations were as: As=23ppb, Co=303ppb, Cd=970ppb, Pb=8510ppb and Hg=25ppb, while no value was recorded in several months. Our results showed no significant differences between stations' means for most of the factors, but different months were significantly different in many factors (P〈0.05). Maharloo Lake demonstrated various levels of pollution regarding to nutrient minerals and heavy metals, but special attention to the relations of water- precipitations is needed for a more concise understanding of its pollution. Results of this investigation can serve as a good record of the lake situation in drought conditions.
    Keywords: Pollution ; Iran ; Maharloo ; Maharloo salt lake ; Saline lakes ; Pollution ; Nutrients ; Heavy metals ; Artemia
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  • 151
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25229 | 18721 | 2018-09-06 06:14:35 | 25229 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Study and recognition of the Quanat water of Rafsanjan, Sirjan and Ravar cities has been beginning since the November of 2004. Among 228 Quanat chains were studied in these cities, 165 quanat chains had a discharge more than 10 liter per second (L/Se) which were sampled. In this research oxygen, pH, discharge, water temperature and air temperature were measured in two times and in the local place. Height from the sea level, longitude and latitude were measured (by GPS Machine) in one time and in the local place and other physical and chemical factors including No_2, No_3, Co_2, total hardness, NH_3 and So_4 were measured one time in the laboratory. In this manner, the condition of connection ways of considered Quanat with city was studied. With examination on the Quanat water in above said cities, all of Quant was within desired extents for aquaculture activities. Moreover in some of them, water temperature was higher than 20 degree centigrade that with some changes and in specific months can be used in aquaculture.
    Keywords: Biology ; Iran ; Rafsanjan ; Sirjan ; Ravar ; Fish ; Aquaculture ; Quant ; Water ; Oxygen ; pH ; Temperature ; No2 ; No3 ; Co2 ; NH3
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  • 152
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25227 | 18721 | 2018-09-06 06:02:34 | 25227 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The major reason application of growth hormone (GH) in fish was commercial use of rapid growth and more replication fish. Numerous studies demonstrated that recombinant bovine somatotropin increased growth rates in several fish species. However, such studies have not been done yet in Iran. This research was taken in two steps. In the first step of experiments, 1200 rainbow trout larvae (Oncorhynchus mykiss) weighing 0.5±0.05 gram were randomly allotted in two groups including: 1) the control group and 2) Eltosil® group, each in three replicate. These larvae were randomly divided in six 100 liter square troughs with a continuous water flow of 1 liter per minute. In the second step of experiments, 600 rainbow trout fry (Oncorhynchus mykiss) weighing 45±1 grams were randomly allotted in two groups including: 1) the control group and 2) Eltosil® group, each in three replicate. These fry were randomly divided in six 2,000 liter round concrete ponds with a continuous water flow of 5 liter per minute. Through and ponds were supplied with a well water (temp. range: 15 2; O2: 7.2 0.2; pH= 8 0.3). In the first step of experiments, the fish were hand-fed with commercial pellets (Biomar) ten times daily at the beginning of the experiments and reached four times daily during continuous growth. In the second step of experiments, the fish were hand-fed with commercial pellets (Cheeneh) four times daily at the beginning of the experiments and reached two times daily during continuous growth. In this research, the fish received of 200g Eltosil® per 100kg BW. The administration method of Eltosil® -mixed feed to fish was once a week for 4 consecutive weeks at each treatment stage, totally 8 times for 12 weeks (once a week, 4 times in the first step; withdrawal for four weeks in the second step; once a week for 4 times in the third step). The aim of this research was to evaluate the effect of Eltosil® (oral recombinant bovine somatotropin 1%) on growth performance in rainbow trout. Accordingly, to calculate growth factors, fish were weighed weekly and their body lengths were measured every other week. At the end of each rearing period (on days 28, 56, 84) the means of whole body weight (WBW), weight gain (WG), average daily growth (ADG), total length (TL), average daily length (ADL), grams of weight gain per gram of food (G/F) in control and Eltosil® groups were calculated. Also, specific growth rate (SGR), condition factor (CF), food conversion rate (FCR), and the percentage of survival ratio in the control and Eltosil® groups were calculated. Moreover, at the end of the rearing period (on day 84), comparative growth rate (CGR), means of WBW, WG, TL, ADL and FCR in the control and Eltosil® groups were calculated. The results obtained in the first step of this research demonstrated that there were no significant differences between growth factors in the control and the Eltosil® groups (p〉 0.05) on days 28; 56; 84 of rearing periods. Also, the results of the second step showed that there were no significant differences between growth factors in the control and Eltosil® groups (p〉 0.05) on days 28 and 56 of rearing periods. However, in second step of experiments, there were significant differences between means of growth factors in WBW (p= 0.019), WG (p〈 0.05), ADG (p= 0.009), TL (p〈 0.001), and ADL (p= 0.04) of the control and Eltosil® groups on day 84 in rainbow weighing 45±1 grams. In the same period, there was greater 11.2% CGR in Eltosil® group than the control group. There was no significant difference between the percentage of survival ratio of the control and Eltosil® groups in any rearing periods (p〉 0.05). In this research generally, oral administration of recombinant bovine somatotropin (Eltosil®) for eight weeks increased final WBW, WG, ADG, TL, ADL, and CGR in rainbow trout fry weighing 45±1 grams on day 84. This data showed that oral administration of recombinant bovine somatotropin (Eltosil®) was a suitable way to improve growth performance in rainbow trout fry weighing 45±1 grams; but it was not in rainbow trout fry weighing 0.5±0.05 gram.
    Keywords: Aquaculture ; Iran ; Recombinant Bovine Somatotropin ; Rbst ; Eltosil® ; Growth Performance ; Rainbow Trout ; Oncorhynchus mykiss ; LG ; Hormone ; GH
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25231 | 18721 | 2018-09-06 05:59:12 | 25231 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Fish is a valuable nutritional source witch use of it in daily meal has a beneficial role on nutritional needs supply and also causes mental and physical health especially in people who have protein and food deficiencies. Unfortunately, per capita consumption of sea foods in Iran is 5.5Kg which is very lower than world standards (18 kg). So, study on fish ice cream formulation, by use of fish protein concentrate (FPC) instead of milk protein, had done to make variation in sea foods products and also increase per capita consumption of these kinds of foods. FPC has very high protein concentration and a lot of necessary amino acids like lysine and methionine. Also its protein is very digestible with highly biological value and it's PER in compare with casein PER is high. At first fish protein concentrate type A produced from silver carp in three steps by the extraction with isopropyl alcohol solvent and heat. Microbiological and physicochemical specifications of produced FPC by rules of FDA and FAO were accepted. Finally according to panel test results, substitution of 30 percent of milk with FPC in comparison with 50%, 70% FPC substitution prepared (P〈0/05). Also microbiological and physicochemical specifications of product were tested and results in compare with national standards of Iran were accepted.
    Keywords: Fisheries ; Iran ; Fish ice cream ; Fish Protein Concentrate ; Silver Carp ; Nutritional ; Amino acids
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25239 | 18721 | 2018-09-07 06:54:42 | 25239 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: To study the preparation of frozen minced meat from abramis brama orientalis commonly known as sim from Arras lake and from silver carp cultured in the Azerbaijan province, and later this minced meat converted to fish ball and fish burger, in this process microbial, chemical and organoleptic tests was done on samples for frozen minced meat on Sims fish the TVN was 10.1 in zero phase and 14.1 after three month and peroxide value from 0.9 t0 3.1 at the same duration. But he value was higher when converted to fish ball, TVN ranged from 14 to17.6 and per oxide from 1.1 to 4.7 after three month, the unit for TVN was mg/100gram and peroxide unit meq/1000 gram, the microbial tests was also resulted in an acceptable range, the meat of Sims fish had no significant difference with the silver carp comparatively only the color and the smell of the fish was little different, so we could conclude that we can use Sim fish meat for frozen minced meat product and this can increase the consumption of fish in the region in the future.
    Keywords: Fisheries ; Iran ; Azerbaijan province ; Aras lake ; Minced fish meat ; Frozen fish ball ; Abramis brama ; Silver Carp ; Culture ; Oreganoleptic test ; Samples
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25627 | 18721 | 2018-10-07 16:48:29 | 25627 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study have been conducted entitle biological and statistical sturgeon in the southern Caspian Sea (Mazandaran waters)” from 2009 to 2013. Sampling was manthly. Overal, 1859 specimens of different species of sturgeon was caught including Acipenserpersicus (69%), A. stellatus (19%), Husohuso (6%), A. nudiventris (4%) and A. guldenstaedtii (2%). Totally, the meat and caviar of five species were found 53263.6 kg and 5633 kg respectively and caviar to meat ratio was 10.6%. The results show that ration of meat and caviar whole species reduced from 2009 to 2012 in which the meat and caviar of A. persicus was declined from 12146 to 4465 kg in meat and caviar from 1358 to 487 kg were extremely reduced respectively. The CPUE (catch per unit effort) of sturgeon has fluctuated from 2009 to 2012. Totally, the CPUE of A. persicus was decreased from 0.150 (2009) to 0.130 (2012) kg boat per day, A. stellatue from 0.009 (2009) to 0.021 (2012) kg boat/day, A. guldenstaedtii from 0.002 (2009) to 0.003 (2012) kg boat/day, A. nudiventris from 0.027 (2009) to 0.001 (2012) kg boat/day and H. huso from 0.123 (2009) to 0.018 (2012) kg boat /day. 1061 specimens of fish has been caviar including A. persicus with 69%, A. stellatus (18.1%), H. huso (5.5%), A. guldenstaedtii (2.7%). The frequency of caviar categorize was included 1 , 2, 3 and massive with 49.6%, 38%), 10.6% and 1.9%, respectively. The highest of meat and caviar belong to A. persicus with 31414 kg and 3515.7 kg, respectively were found and the lowest was 841kg and 105.9 kg pertain to A. guldenstaedtii. The results show that 65.9% of sturgeon was caught by legal catch (landing) and 34.1% by beach seine remained. 391 individuals was age determined that the minimum and maximum age was 9 and 41 year comprised to H.huso and A. stellatus, respectively. A. persicus and A. stellatus were majority caught compare to other species. A. persicus has the highest frequency in 14-15 year age groups with 50% and 57% in 2009 and 2011, respectively and 15-16 year age group with 41% and 56% in 2010 and 2012, respectively. The results show that mean of fork length, body weight, caviar weight of A. persicus was declined because the FL and weight average (±SD) obtained from 152.4±16.7cm and 25.8±9.2 kg in 2009 to 146.5± 18.7cm and 22.1±9.2kg in 2012, respectively. In contrast, the average (±SD) FL and weight of H. huso was increased from 218.8±41.1cm and 137.3±96.6kg in 2009 to 231.3±45.2cm in FL and 146.2±78.5kg body weight in 2012, respectively. Consequently, the results show that stocks of sturgeon in the Caspian Sea has a trend declined because of some species in IUCN categorize list was Endangered in 2006 but at this moment lie in Critically Endangered list of IUCN categorize were down which could be a serious alarm for the valuable sturgeon fish in the Caspian Sea. Therefore, recommendation that trade catch of sturgeon in the Caspian Sea must be forbidden and continue legal catch for spawner fish that needede to artificial propagation only.
    Keywords: Biology ; Iran ; Caspian Sea ; Biology ; Sturgeons ; A. persicus ; A. stellatus ; A. guldenstaedtii ; A. nudiventris ; H. huso ; CPUE
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25250 | 18721 | 2018-09-06 06:29:01 | 25250 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: An artificial Reef is a fabricated, underwater structure, typically built for promoting marine life in areas of generally featureless bottom. Creating artificial reefs began from 2000 in southern provinces in Iran. This study has surveyed about the Bushehr and Khozestan states. The objective is to determine the effectiveness of artificial reefs in attracting and enhancing the production different aquatics through increasing of fish assemblage and fishery. The performance of artificial reefs deployed off the coast of the Persian Gulf in 10 to 15m, was evaluated. The reefs comprised of seven artificial and one control (natural) statistical tests plan in three replicates. Three types of artificial reefs were used in this study. That two were designed reefs and one was not designed or used material. The experimental plan consisted (i) Reef ball (R), designed hemispherical shaped; (ii) Laneh Mahi (L), designed pyramid shaped; (iii) Used material (U); (iv) mixed (RL); (v) mixed (RU); (vi) mixed (LU); (vii) mixed (RLU); and (viii) control site (CS). Trap nets and under-water visual census surveys were adopted for seasonal sampling of fish aggregation. Results of statistical analysis using ANOVA and T-test of the mean Catch per Unit Effort (CPUE) showed significant difference (p〈0.05) in term of computing number of fish for aggregation of fishes. The study has concluded that reef deployments have influenced favorably the fish communities and fish harvests. Therefore, the artificial reefs, especially the mixed RLU, are appropriate tools for future fishing enhancement in the Persian Gulf of Iran.
    Keywords: Aquaculture ; Iran ; Persian Gulf ; Khozestan Province ; Bushehr Province ; Artificial reefs ; Fisheries ; Marine ; Survey ; Hemispherical ; ANOVA ; CPUE ; Underwater structure
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25245 | 18721 | 2018-09-07 07:22:16 | 25245 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Annually, many data of hydrology and hydrobiology of the southern Caspian Sea at Iran's coasts were collected by Caspian Sea Research Institute of Ecology. The data which had been collected by researchers submitted annually in several reports but has not been processed all at once. Fluctuations of various parameters were surveyed and the relationships between them were analyzed. The main goal of this research is to analysis the data were obtained from 1994 to 2006 and show changes the concentration of various parameters in the Caspian Sea. Physicochemical data indicated that the average trend of transparency and salinity was decreased from 1994 to 2006 annually, where the salinity value was decreased from 12.37 ppt (part per thousand) to 11.5 ppt. The average of dissolved oxygen was increased from 6.4 to 8.6mg/l. pH has slightly fluctuated from 8.15 to 8.31. However, with increasing depth from surface to bottom, the average of dissolved oxygen was sharply decreased (DO concentration in surface and 800 m was 7.18 and 1.45 mg/l, respectively). The trend of salinity and pH fluctuation was very slow with depth but the salinity has an increasing with incrementing of depth but pH has a decreased trend. The average of organic and inorganic phosphorus and TP was nearly increased. The average of Nitrate and Silica sharply increased comparing to depth but the average of Nitrite and organic Nitrogen decreased. The results showed that the temperature fluctuation in different water layers was low in winter but the dramatic decreasing of temperature was occurred at 10-20 m (in spring), 20-50 m (in winter) and 50-100 m (in autumn). With increasing of depth (Slope of the Sea), transparency and salinity values have an increasing while DO and pH showed decreased. As a whole, 335 species of phytoplankton were identified, there are 70 species in advance as these species were not seen in this period but 96 of novel's species have been recorded. The lowest and the most of phytoplankton biomass was recorded in 2006 and 2001 while the biomass was 59 and 1034 mg/m^3, respectively. Albet, the lowest biomass was recorded at the depth of 100 m (24.1 mg/m^3) but the maximum was observed in surface layer (1344.1 mg/m^3). The average of phytoplankton biomass was increased after arrival of the ctenophore. There was a significant difference between the average of biomass in different seasons before and after of the ctenophore invasion (P〈0.001). Bacillariophyta and Pyrrophyta have the most cell abundance with a total of biomass of 52.7 and 37, respectively. From 1994 to 2006, species diversity (Shanon-Niner), evenness and richness were estimated between 3.02-1.29, 0.28-0.61 and 4.32-7.60, respectively. A total of 65 zooplankton species were identified with a frequent species in the Caspian Sea before the ctenophore invasion, while the species diversity decreased after the invasion. The high and the lowest of species diversity was recorded in 1994-1995 and 2006 and in 1999 the biomass of the zooplankton were observed between 12.6 mg/l and 363.8 mg/l, respectively. After arrival of ctenophore, the averages of zooplankton biomass at of the different depths were sharply decreased and were less than the ctenophore invasion. The result showed that there was a significant difference among the average of zooplankton biomass in two periods, seasons and west, middle and east regions as follows: P〈0.001, P〈0.008 and P〈0.01. The maximum abundance and biomass of zooplankton belonged to copepoda with %51.5 and %63, respectively. Rotatoria falls in the second class from 1994 to 2006 (during 1994-2006), species diversity, evenness and richness were varied between 0.19-1.6, 0.08-0.52 and 0.37-2.29, respectively. The species diversity of macrobenthic organisms at the same period s followd by zooplankton changes which sharply decreased. The average of benthic biomass reduced from 13.7 g/m^2 in 1994-1995 to 1.8 g/m^2 in 2004 but sharply increased in 2005 with a maximum value of 46.7 g/m^2. With increasing of depth, the average of benthic biomass was sharply decreased. There was a significant difference (P〈0.001) in macrobethic organisms abundance before and after the ctenophore invasion, but it did not show a significant difference between seasons and different regions (as follows: P〉0.137, P〉0.782). Before the presence of ctenophore, the worms had been constituted a dominate group with an approximately %45 of total abundance and also %20 of their biomass. After this period, their frequency was sharply increased (more than %85) but the maximum value pertains to Cardidae (more than %90). The species diversity, evenness and richness were varied between .69-2.51, 0.23-0.63 and .011-3.79, respectively. AMBI software, Shanon-Viner parameter was moderate in all seasons from 1994 to 1996 but this parameter was reduced in a bad limit (boundary) in the most seasons from 2002 to 2005. The main parameter of M-AMBI consists of several parameters and also approximately showed similar changes such as Shanon -viner parameter. On basis of this parameter, the ecological quality condition of Caspian Sea was superior limit in all seasons from 1994 to 1996 but after the following years were decreased from good to moderate limits (After the presence of ctenophore). Relationship between abiotic variants and phytoplankton indicated that there was a direct relationship between transparency and salinity while there was a powerful and reversal significant relationship between transparency water temperature, transparency in organic Nitrate, phytoplankton number and transparency phytoplankton biomass. The comparison between different variants average in two periods (before and after the ctenophore invasion) indicated that the average of Kilka catch was decreased, the relative frequency of clupeonella engrauliformis and clupenoella grimmi was sharply reduced but the relative frequency of Clupeonella cultriventris was sharply increased, the species diversity, evenness and richness and the number of zooplankton species were sharply decreased, the average of biomass and transparency was sharply reduced, the average of dissolved oxygen and liza saliens or liza auratus catch were increased. These difference were often significant (P〈0.05). Therefore, with the ctenophore invasion into the Caspian Sea, the primary production was increased, the biomass value of zooplankton and specially Kilka which fed on zooplankton were sharply decreased while the fish such as Rutilus frisii kutum and mullet (Liza salins or Liza aurratus) which fed on benthic were increased.
    Keywords: Ecology ; Iran ; Caspian Sea ; Transparency ; Salinity ; Dissolved oxygen ; pH ; Nitrate ; Nitrite ; Species ; Phytoplankton ; Biomass ; Bacillariophyta ; Pyrrophyta ; Diversity ; Zooplankton ; Rutilus frisi kutum ; Liza salins ; Liza aurratus
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25253 | 18721 | 2018-09-07 06:53:45 | 25253 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Acipenser persicus, one of the native Species living in the southern part of the Caspian Sea, has high Percentage of larval mortality. Therefor to decrease the rate of mortality of larvae survival and to increase to regenerate properly during the active feeding process of fish larvae, while under artificial reproduction they were fed withe fresh water rotifer. Four types of feeding treatments were applied as follow: Treatment 1: Similar to normal process, first withe Artemia cyst then withe Daphnia Treatment 2: Mixture of Artemia, Rotifer and Daphnia; Treatment 3 freshwater Rotifera ;Treatment 4: fresh water Rotifer enriched with Vitamin C (Ascorbic acid- 6- Palmytat).Three replicates were used for each treatment. In the process of each observation 45 larvae in containers withe 100 liter capacity filled with 30 liters of water, after 8 days of external feeding process, were inspected closely, during which , they were under biometric measurement once every two days. Larvae were fed Four times a day at a rate of 25% of their body weight. The average temperature estimation was 22.5±0.5 centigrade, pH .of water being 85±0.1 while Oxygen proved to be 9.58±0.2 mg/l. At the end of the observation, the profile fatty acids of the remains were studied. The growth factors, the mouth size of the larvae and the expansion of the mouth size compared to the total length of the larvae during the whole observation were inspected. Credibility of data designated was examined by Shapiro Wilks test. Specific growth rate (SGR), weight growth (WG) and feed conversion ratio (FCR) were contemplated by one-way analysis test, and significant difference Tukey's test. The most notable diversity was evident between treatment 2, whose indicative factors were in the following order: 4.65± 0.06, 45.18±0.66, 4/48 ± 0/07 and treatment 4 whose indicative factors showed the following results: 10.47±0.04, 124.42±0.62, 1.51±0/008.For the determination of fatness rate and weight gains, Kruskal Valis and Man vit ni test were used .In this part of study , the highest diversity fatness appeared to be between treatment 4 ,reflecting 0.79±0.07 and treatment 2 showing the result 0.62±0.05.The highest gain was related to treatment 4, whit the rate of 99.33±0/68 mgr and the lowest gain occurred in treatment 2 white the rate 62.36 ± 0.65 mgr. This study also revealed that, the survival ratio connected with treatments 3 and 4, compared treatments 1 and 2, were significantly different. The profile of fatty acids also showed much higher percentage of PUFA and HUFA in treatments 3 and 4, than those in other treatments. Furthermore Brachionus calyciflorus proved to be a proper food supply for larvae. Therefore by building suitable pools next to the work side, other kinds of species fresh water rotifer could also be used as food improvements for feeding larvae.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Brachionus calyciflorus ; Acipenser persicus ; Fatty acid ; Larvae culture ; Vitamin C ; Larval survival ; Species ; Mortality ; Feeding ; Artemia ; Cyst ; Ascorbic acid ; Oxygen ; Growth ; SGR ; FCR ; Fresh water
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25256 | 18721 | 2018-09-07 07:21:26 | 25256 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Sampling was done using 90 post larvae which were produced by reproduction of some broodstock of Penaeus indicus in one day and reared in the same situation for 4 month. Samples were classified in 3 group high growth, medium and low (according to their weight and length). Genomic DNA was extracted from a 1cm^2 piece of muscle using the phenol-chloroform method. The polymerase chain reaction (PCR) was done using 21 RAPD loci. And PCR products were separated on 3% Agarose gels. From 21 studied loci, 12 produced polymorphic bands. The most polymorphic band produced using OPAQ 9 and the least by OPAQ 7. According to Nei 1972, the highest distance (0.457) was between low growth group and medium and the lowest (0.091) between high growth group and medium, therefore the highest identity (0.912) was between high growth group and medium and the lowest (0.633) between low growth group and medium. Consensus neighbour-joining tree using Nei (1972 and 1978) resulted in two clades, the first including high and medium growth groups and the second low growth group, it appears that low growth group are depended on separated population of the two others. With considering of mean weight of F1(16.25±1.5), mean weight of 15 ±1.2 and mean weight of parent 31.6, response to selection (R) and heritability for growth in this species were estimated 1.2±0.2 and 0.07±0.01 respectively. In another part of this study Sequencing of specific bands and primer design were done and examining of them on the same age specimens is necessary in following.
    Keywords: Biology ; Iran ; Penaeus indicus ; High growth ; RAPD marker ; Genetic ; Shrimp ; Breeding ; Culture ; Sampling ; Broodstock ; Phenol-chloroform methods ; Population ; Species ; Specimens
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    Publication Date: 2021-07-16
    Description: For the accomplish of project, nine treatments with three replicate in each treatment, used as follows. Control treatment: Feeding with natural foods (Chaetoceros sp and artemia nauplii) Treatment 1: Feeding with combination of inner artificial diet and natural foods. Treatment 2: Feeding with combination of imported artificial diet and natural foods. Treatment 3: feeding from zoea 1 to mysis 1 with chaetoceros sp and from mysis 1 to pl15 with inner artificial diet. Treatment 4: feeding from zoea 1 to mysis 1 with chaetoceros sp and from mysis 1 to pl15 with imported artificial diet. Treatment 5: feeding from zoea 1 to pl15 with project artificial diet. Treatment 6: feeding from zoea 1 to pl15 with imported artificial diet. Treatment 7: Feeding in zoeal stages with chaetoceros sp and from mysis 1 to pl15 with chaetoceros sp+artemia nauplii+ imported artificial diet. Treatment 8: Feeding in zoeal stages with chaetoceros sp and from mysis 1 to pl15 with chaetoceros sp+artemia nauplii+ project artificial diet. Larvae stocked at a density of 100 nauplii/ L-1 (1000 nauplii in each tank each tank), at the beginning of culture period The results showed that, in treatments 1, 2, 7 and 8 from zoea 1 to pl15, growth indexes were suitable and noticible, and in most treatment better than control treatment. Survival percent, in pl15 in treatment 1, 2, 7 and 8 was more than control treatment, and differention between treatment 1, 2 and 8 in comparision with control treatment was statictically significant (p〈0.05). Survival percent in treatments 5 and 6 from stages zoea 1 to late zoea 3 stage, in comparision with other treatments were lesser, but without statictically significant (p〉0.05). But thereafter, significantly decresed. In treatments 3 and 4 from zoea 1 to late mysis 3 stage, survival percent in comparision with other treatments were suitable, and thereafter, significantly decreased, and with statictically significant with other treatments (p〈0.05). The means of final weight in treatments 1, 3 and 8 was more than control treatment, but without statictically significant (p〉0.05), but in treatment 8, was more than other treatments. The mean of final length between treatments 1, 2, 6, 7, 8 and in comparision with control treatment, were not statistically significant (p〉0.05). But in treatment 2, more than control treatment, and in other treatments less than control treatment.
    Keywords: Aquaculture ; Iran ; Larvae culture ; Litopenaeus vannamei ; Imported artificial diet ; Inner microbound artificialdiet ; Feeding ; White shrimp ; Larval stage ; Chaetoceros Sp. ; Artemia nauplii ; Zoea ; Mysis ; Diet
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25651 | 18721 | 2018-10-14 02:48:20 | 25651 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was conducted to codify the guideline and indexes of broodstock management in salmonid fish and cryopreservation, selective breeding and sex reversal in hatchery to attain responsible aquaculture development. Cryopreservation includes of sperm and egg in term of economic is important in aquaculture. In this report mentioned the type and name of material. All female and sex reversal fish are produced by steroid hormone which mentioned in this report. Selective breeding include individual selection and family selection which could be used for genetic improvement of fish.
    Keywords: Aquaculture ; Biology ; Management ; Iran ; Broodstock ; Reproduction ; Cryopreservation ; Genetic ; Breeding ; Aquaculture ; Salmonid fish ; Sex reversal ; Hatchery ; Sperm ; Egg ; Female ; Steroid hormones
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25649 | 18721 | 2018-10-08 08:09:48 | 25649 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was conducted for the first time and the effects of probiotic bacteria isolated from the gut of Rainbow trout (Oncorhynchus mykiss). In the first step, after the purification of the isolated bacteria, probiotic properties in vitro was assessed with injection to healthy fish and challenge with three species of pathogenic bacteria in culture media. The second phase of the bacteria isolated in 5 treatments (log 7, 8 and 9 of the lactic bacteria (LAB), Vibrio sp and Pseudomonas sp) and a control treatment on growth and survival, hematological parameters (RBC and WBC cells), immunology and physiology parameters tested during the 60 days of the above parameters after 30 and 60 days and finally at the end, the final assessment was Streptococcosis resistance in fish. The results showed that the isolated bacteria were able to enhance the growth parameters (weight, feed conversion, feed fat, protein efficiency ratio and specific growth rate) and survival. The results showed that log 8 LAB was significant difference with other treatments and control. When using log 8 LAB and Vibrio, the MCV, MCH and MCHC were decreased with no significant difference. The log 8 has the greatest effect on the amount of liver enzyme (AST), IgM and complement component C3, and had significant difference with other treatments. The challenge examination to Streptococcus iniae showed the highest survival in treatments with log 8 (96.66%), and Vibrio (93.33%) and then subjected to other treatments and control are also the least survival (25.38%). The conclusion of that study is the first probiotic properties of bacteria isolated from trout to changes in quality indicators are in particular the LAB bacteria and the second log 8 of LAB had significant positive of changes development and safety, and the fish are resistanted against Streptococcosis.
    Keywords: Aquaculture ; Health ; Iran ; LAB ; Rainbow trout ; Growth ; Immune system ; Streptococcosis ; Bacterial ; Oncorhynchus mykis ; Pathogenic ; Survival ; Hematological parameters
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25642 | 18721 | 2018-10-14 02:40:17 | 25642 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Padina boergesenii is one of the most abundant brown algae distributed in the north of Persian Gulf and Oman Sea. In this study after sampling and preparation of Padina boergesenii by Chroform-Etanol (3-1) solvent and by Methanol has been extract. Separation and purification of the compounds was carried out using thin layer, general and inverse column chromatography, Cephadex and high-performance liquid chromatography (HPLC). Structural elucidation of the constituents was based on the data obtained from H-NMR, 13C-NMR, HSQC, HMBC, DEPT and Cephadex LH-20. The steroids compounds separated from above alga were identified as 22dehydrocholesterol (1), cholesterol (2), fucosterol (3), β-sitosterol (4), stigmasterol (5), ostreasterol (6) and two epimer of hyroxyestrol(7), based on their spectral data and from comparison with those previously reported in the literature.
    Keywords: Biology ; Chemistry ; Iran ; Oman Sea ; Persian Gulf ; Brown Algae ; Padina boergesenii ; Steroids compounds ; Extraction ; Purification ; Identification ; Amount verification ; Sargassum glaucescens ; Algae ; Abundant ; Sampling ; Chromatography ; Cephadex ; Dehydrocholesterol ; Cholesterol ; Fucosterol ; β-sitosterol ; Stigmasterol ; Ostreasterol
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25665 | 18721 | 2018-10-14 03:04:11 | 25665 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was carried out about 15 months to determine suitable locations for the development of aquaculture across the Gamasiab river of Hamedan province in 2007 - 2008. Physicochemical parameters and macro benthic invertebrates fauna 15 and 13 stations respectively every 45 days and seasonally fish identify in 8 stations were performed according standard methods. The average water temperature was 12.8±3.7 oC, minimum and maximum dissoled oxygen were 2.6 and 12.5 mg/lit respectively during the study. The pH range was between 7.5-8.8 , the minimum and maximum Ec was 250 and 750 µmos/cm in Mehr and Tir. The transparency range was recorded about 1-103 F.T.U. The uder factors such as amount of suspended materials: 0.01-0.1 mg/l, amount of BOD5: 0.05-6.2 mg/l and COD: 30 (minimum) and 70.57 (maximum) were recorded. The total solids material (T.S.S) were less than 1 mg/lit. Totally 37 groups of bentic organisms were identified which 11 groups of them were appertained to pollution sensitive orders containing Ephemeroptera, Trichoptera and Plecoptera. During fish population studied 21 species belonging 5 family were identified. Based on obtained results of biological, ecological, economic and social parameters, 10 sites in 7 areas were suitable identified for fish rearing and 12 aquaculture programs (10 plan for fish rearing and 2 plan for increasing fish production of (Oncorhynchus mykiss). The minimum and maximum production capacity in mentioned places was estimated about 1675 and 3850 tons respectively.
    Keywords: Aquaculture ; Iran ; Hamedan province ; Gamasiab river ; Feasibility ; Complexes of fish farms ; Biotic and Abiotic factors ; Oncorhynchus mykiss ; Rainbow trout
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25784 | 18721 | 2018-10-13 08:36:54 | 25784 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The effects of one diet contain of 42 percent soybean meal (experimental treatment with 38 percent crude protein) in comparison with commercial diet (control treatment with 39 percent crud protein) at 6 earthen ponds, each one with the area of 0.4 ha, ( 2 treatments and 3 replicates in each treatment) on growth indexes of western white shrimp (Litopenaeusvannamei) was determined. The initial weight of post larvae was 0.008±0.001g, with density of 25 ind/m^2 . The mean of culture periode in the experimental treatment and control treatment was 115 dayes, The mean of growth rate (GR) in the experimental treatment and control treatment was 15.70±0.88g and 15.60±0.52 g, respectively and significant statistically difference was between those (p〈0.05). The food conversion ratio (FCR), in the experimental treatment and control treatment was 1.80 ±0.08g and 1.76±0.06 g, respectively, and significant statically difference was between those (p〈0.05). Specific growth rate (SGR) in the experimental treatment and control treatment was 2.38 ±0.03 percent and 2.38 ±0.04 percent, respectively and significant statically difference was between those (p〈0.05). The average daily weight gain (ADG), in the experimental treatment and control treatment was 0.136 ±0.007g and 0.135±0.004 g respectively, and no significant statistically difference was between those (p〉0.05).The net protein utilization (NPU) in the experimental treatment and control treatment was 17.05 ±0.38 percent and 11.80±0.26 percent respectively, and significant statistically difference was between those (p〈0.05). The yield of shrimp in experimental treatment and control treatment was 2853.58 ±64.14 kg/ha and 2864.83±168.57kg/ha respectively, and significant statistically difference was between those (p〈0.05). The survival in the experimental treatment and control treatment was 91 ±1.78 percent and 92±2.41 percent, and no significant statically difference was between those (p〉0.05).The amount of diet consumption, in the experimental treatment and control treatment was 5144±112.23 kg and 5055±59.77 kg respectively, and significant statistically difference was between those (p〈0.05). The price of 1 kg of plant diet and commercial diet computed 34.000 rials and 43.000 rials respectively. In the shrimp body analysis, crude protein percent, in the experimental treatment was more than control treatment and significant statistically difference was computed between those (p〈0.05). Totally, the use of plant protein for the feeding of L.vannamei in the earthen pond, can decrease the cost of plant diet, 20 percent lower than commercial diet.
    Keywords: Aquaculture ; Iran ; Plant diet ; Commercial diet ; L.vannamei ; Earthen Pond ; Growth indexes ; Litopenaeus vannamei ; Protein ; Diet ; Western white shrimp
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  • 166
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25793 | 18721 | 2018-10-13 08:12:04 | 25793 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This feasibility study carried out in response to request of Tehran Province Fisheries Management, in order to Qanat Salehabad water assessment for aquaculture relataed purposes in Tehran Municipality – Regions 19 & 20. During 6 monthes between May – October 2011 in 3 sampling stations by 3 replicates investigations executed. Physic-chemical, Heavy methals, organochloro-organophosphore pesticides and microbial nominated parameters analysed in reference to Iran national and international standards as well, resulted an overall assessment on quality and quantity of Qanat water for aquaculture purposes. Results expressed station 1 in all studied seasons can be develop for fisheries warmwater applications and in cold seasons (September-March) fisheries coldwater relevant activities can be consider. Stations No 2 & 3 known as infeasible for fisheries applications but would be recommend for agriculture rrigation. According to the results, possibility of developing live fish shopping centers (Cold – warmwatetr fishes) between station 1 and before crossing the Qanat flow and Behesht-e-Zahra freeway is considerable, indeed water rights could be negotiate between Tehran Municipality and Tehran province Fisheries Bureo. Also application of feasible anti microbial filters and other minimum limitation parameters control is necessary before leading Qanat water flow into proposed live fish shop. And suitable septic tank is recommended at the outlet of proposed fisheries units. Monitoring of nominated physic-chemical & microbial parameters during operation of fisheries unites highly recommended.
    Keywords: Aquaculture ; Iran ; Tehran province ; Salehabad ; Qanat ; Aquaculture ; Fisheries ; Fish ; Physicochemical ; Heavy metals
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25792 | 18721 | 2018-10-13 08:54:26 | 25792 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Cheshmehkileh River and adjacent mountainous streams, play a strategic role as a historical axis for anthropogenic civilization, human welfare also habitat and migration pathway of commercial – biologic valuable fishes e.g. Caspian trout, Caspian kuttum, members of Cyprinidae family in south Caspian Sea drainage. Treats such as overfishing of Caspian trout and Red spotted trout stocks in mountainous headwaters, barriers construction and manipulations those are out of river carrying capacity developed by human activities, affected normal function of river as well. Sand mining big factories establishment next to the river, legal and illegal trade of river sediments, direct entry of Tonekabon landfill leakage into the river, development of Rainbow trout farms since 3 decades and huge effluents into the river containing dead fish and types of solids, escapement of cultured Rainbow trouts into the river, … are major minimum factors which needs basic information for integrating inclusively drainage management system. Cheshmehkileh River contains Headwaters of Dohezar (Daryasar & Nusha), Sehezar and Valamroud rivers during 13 monthly sampling phases between September 2009 and October 2010 based on macrozoobenthoses investigations by EPT, EPT/C EPA protocols, measurements of nominated physic-chemical and microbiologic parameters. Probability of Rainbow trouts escapement and invasion, existence, nutrition in Cheshmehkileh environment indeed investigated. Data analysis explained significant differences (P〈0.05) between groups of measured parameters in different sampling stations. Dendogram of clustered analysis based on consolidation of major biologic/ physic-chemical and microbiologic parameters, separated stations No. 1, 3, 2, 4 in one group and remained classified in different groups. Station 8 and 9 similarly separated which expressed general similarities according to Sehezar river environment which were differs in comparison with other stations. Station 11 separated according to its natural quality of water and environment. Similarities between station 10 to Sehezar river stations 8 and 9 expressed general influence of Sehezar River more than Dohezar River in Cheshmehkileh condition especially in station No. 10. High scores of EPT and EPT/C indices in upstream stations 1, 3 and 8 also low score of indices in stations 7, 13 and 6 expressed levels of environment quality between these groups of stations. Maximum average biomass of macroinvertebrates belongs to Trichoptera order in Cheshmehkileh River. Significant decrease of biomass in stations 11, 12 and 13 in comparison with other stations stated environment degradation in mentioned stations relevant to excessive sand mining as well. Pollution resistant groups of invertebrates significantly increased in downstreams against upstream stations. Also disappearing of Plecoptera order in station No. 7, 9, 10 and 13 stated low quality of environment in comparison with upstream stations. Confirmation of effects quality and quantity for point and non-point sources of imported pollutants require specific management considerations in order to present exploitations, pollutants control and emergencies for river monitoring in forthcoming years.
    Keywords: Aquaculture ; Management ; Iran ; Tonekabon ; Cheshmehkileh ; River ; Pollution ; Aquaculture ; EPTC ; Assessment ; Macroinvertebrates ; Chemistry ; Microbiology
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  • 168
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25795 | 18721 | 2018-10-13 08:20:21 | 25795 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This experiment was conducted to evaluate the efficacy of different levels of Garlic (Allium sativum) methanol extract on the growth performances (final weight (FW), daily growth ratio (DGR), feed conversion rate (FCR), voluntary feed intake (VFI) and protein efficiency ratio (PER) and some of hematological parameters (hemoglobin (Hb), hematocrit (Hct), red blood cell (RBC) and white blood cell (WBC) of grey mullet (Mugil cephalus). The experiment was conducted in a completely randomized design with 360 of larvae (with an average weight of 0.75±0.03g) in 4 treatments: control group without using Echinacea extract, another group (treatment 2, 3 and 4) the amounts of this extract were 50,100 and 200 g/kg food. The highest FW (4.22±0.11g), DGI (1.72±0.50%) and the lowest FCR (0.95±0.05) and VFI (1.77±0.05%), were observed in treatment 4. But treatment 4 in all of these growth parameters did not show a significant difference compared with treatment 3 (P〉 0.05). After 60 days, treatments 3 and 4, showed significantly higher RBC, WBC, Hb and Hct than those fed the control diet. Finally, the present results suggest that diet containing 100 and 200 g kg-1 extract could improve growth and hematological parameter of M. cephalus.
    Keywords: Aquaculture ; Health ; Iran ; Mugil cephalus ; Allium sativum ; Methanol extract ; Growth yield ; Hematological parameters ; Immunity ; Larvae
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  • 169
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25817 | 18721 | 2018-10-13 10:00:51 | 25817 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Cheetgar Lake or Persian Gulf Martyrs' lake is an artificial lake in the North west of Tehran is located in district 22 of Tehran municipality. The lake covers an area of 130 hectares, which is located north of forest park Cheetgar. In south, to Tehran-Karaj highway, In north Hemmat expressway, from East to Azadegan expressway and west and from residential areas district 22 of Tehran municipality is limited. Kan River from East and Vardavard of West cheetgar Lake crossing and Kann River is the main source of water of the Cheetgar Lake now. Plankton is one of the important factors related to water quality. In this context, understanding the biological and nonbiological lake and its ecological status of a useful tool for managing sustainable exploitation with an emphasis on water quality is maintained. This study focused on phytoplankton structure, a bioticlimiting factors in phytoplankton bloom at the 5 stations between 2013 and 2014 in the Chitgar Lake. according to profile Lake after visiting five stations in the lake water body determined that Different depths are sampled. Sampling of phytoplankton is done by using a liter Ruthner sampler in layers and deep level (of a cylindrical column) were taken. Than 4% formalin fixed samples and transferred to the laboratory for quantitative and qualitative study. In laboratory planktonic samples after determining the volume and mixture, were transferred by pipette to 5ml chambers and after sufficient time to sediment, were identified and counted by inverted microscope. This study identified 35 phytoplankton taxa comprised of diatoms (12 genus), chlorophytes (15 genus), cyanophytes (4 genus), dinoflagellates (2 genus) and chrysophytes (1 genus) in the lake. The finding showed, the diatom abundance dominate (average of 2060000 ± 230000 cells.l-1) in the Chitgar lake. The annual average phytoplankton abundance was calculated as 2550000 ± 304000, with the maximum value recorded in February 2013 (4400000 ± 450000 cell. l-1). The PCA analysis displayed, the diatoms Cyclotella sp., Achnanthes sp. and Chrysophte Dinobryon sp. were dominated in the study period. Based on CCA analyses, total nitrogen and water temperature were the significant parameters to increase cyanophytes and dinoflagellates abundance in the Chitgar lake. In overall, the lowest phytoplankton abundance recorded in the Chitgar Lake as compared with other lakes which is an meso-oligotrophic category with the latest trophy state lake. Thus, it is might be increased eutrophication trend due to no management and no aquatic control in this ecosystem.
    Keywords: Ecology ; Iran ; Persian Gulf ; Density ; Distribution ; Gulf Martyrs Lake ; Phytoplankton ; Meso-oligotrophic
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  • 170
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25807 | 18721 | 2018-10-13 10:13:41 | 25807 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: White spot disease (WSD) is one of the shrimp deadliest viral diseases that causes heavy losses on all shrimp of Penaeid family. Most invertebrates are lacking adaptive immune system and its defense is the innate immune system that is as cellular and humoral, but a like immune system against white spot virus in shrimp was been detected. In this research, control and prevention of white spot disease in shrimp using algae Gracilaria corticata, investigated. About 300 vannamei shrimp were divided to 4 groups and fed by normal pellet and algae extract in 14 days. At the end of the fourteenth day half of the shrimp were challenged with acute white spot virus. After the fourteenth day in the days 0, 3, 9, 18 and 25 sampling were done from the hemolymph of survived shrimps and survival and immune factors were evaluated. Based on results, in the challenge test, shrimps that fed with algae extract has a significant survival rate than shrimp fed with commercial diet. Increased the Immune Factors from day one to day 25 observed during the test. Greatest amount of Immune Factors THC, TPP, SOD, POD and PO in T1 group were observed in day 25 of tests. This situation was also true for group2 (T2), but its rate significantly was less than group 1(T1).
    Keywords: Aquaculture ; Health ; Iran ; Evaluation ; Immunity factors ; THC ; TPP ; PO ; SOD ; POD ; Shrimp ; Algae ; Gracilaria corticata ; White spot virus
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25816 | 18721 | 2018-10-13 10:21:41 | 25816 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Saprolegnia sp. is the most important pathogenic fungi in fish. Saprolegnia parasitica, S. diclina, S. ferax and S. hypogina are the most abundance of the fungi that caused saprolegniasis in fish and hatcheries.They have global distributions and their percences were reported from cold water fish farms of the world. It has been amazingly shown, 1 in 10 hatched salmon raised in fish farms die as a result of S. parasitica infection. Saprolegniasis caused mortality not only in cultivated cold water fish but also in wild cold water fish and the disease were killed up to 22% of wild salmon. Up till 2002, Saprolegnia infections in aquaculture were kept under control with malachite green, an organic dye that is very efficient at killing the pathogen. However, the use of malachite green has been banned worldwide due to its carcinogenic and toxicological effects and this has resulted in a dramatic re-emergence of Saprolegnia infections in aquaculture. The economical losses of Saprolegniasis are so high and many investigations were conducted on identification, virolence and predisposing factors, prevention and control of saprolegniasis in the world. This study has been focused on the last data about ofn physiological characteristic, identification methods, and pathogenicity of Saprolegnia sp., virulence parameters, predisposing factors, prevention and control of saprolegniasis.
    Keywords: Aquaculture ; Iran ; Saprolegniasis ; Cold water fish ; Pathogenisithy ; Malachite green ; immunostimulants ; Propagation ; Cultivation
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  • 172
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25820 | 18721 | 2018-10-13 10:11:10 | 25820 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Aquaculture activity is restricted mainly to rainbow trout in cold waters and carps in warm waters in inland waters of Iran as well as Fars Province. Probably for this reason saline waters of south and southeast Fars have not been used for aquaculture. These rivers are ecosystems with a diverse biota, it is predictable that can be found some there endemic aquatic organisms with a potential for aquaculture. Finding a suitable organism can help increase wise use of this natural resource, and local production and recruitment. However, it is highly needed to investigate various environmental elements prior to any use to obtain an estimation of the environmental consequences, parallel to finding usage potentials. In This study, we investigated Dehram saline river in south of Fars Province. Hydrological and physiographical properties, chemical composition of the river, and aquatic populations were sampled and measured monthly in 3 stations in one year. Salinity, electrical conductivity, and total dissolved solids increased from station 1 to 3, reaching from 6.6 g/L, 14.3 g/L, and 12800 µs/cm to 13.5 g/L, 17.7 g/L, and 16500 µs/cm, respectively. Dissolved oxygen and oxygen saturation were higher in station 3, and in autumn and winter. Nitrite showed lowest values in winter (0.006 mg/L) and highest values in spring (0.013 mg/L). Nitrate showed the lowest concentration in station 3 (0.43 mg/L), but no differences were seen among seasons. Ammonium showed no differences among stations or seasons. Phosphate levels in station 1 and 3 were 0.13 mg/Lto 0.17 mg/L, with the highest level (0.29 mg/L) seen in the spring. Five species of fishes were identified in the river, which all were widely favored by local people. Some of these have been categorized as edible in previous studies, others as ornamental. On the other hand, intensive growth of an algal species throughout the river demonstrates a potential for algal cultivation. Significant role of algae in production of food for both man and livestock is now well recognized in aquaculture industry.
    Keywords: Ecology ; Iran ; Fars Province ; Dehram River ; Limnology ; Saline water ; Physico-chemical parameters ; Aquaculture
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  • 173
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25818 | 18721 | 2018-10-13 10:23:49 | 25818 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The ecological study of the Persian Gulf Martyres Lake carried out at the 5 stations between 2013 and 2014. This study identified 35 phytoplankton taxa. The diatoms taxa was dominated and their abundance recorded highest (2000 000 cell. L^-1) in the lake. The annual phytoplankton abundance was measured as 2500 000 cell. L^-1 during the study. The total nitrogen and water temperature were the significant a biotic parameters to increase cyanophytes abundance. Furthermore, 37 zooplankton taxa were identified. The Rotatoria abundance was dominated zooplankton. The annual zooplankton abundance was measured as 72 ind.l-1. Based on the CCA, there was no correlation between Rotatoria abundance and a biotic parameters. The study benthos showed Ephemeroptera and Diptera abundance were dominated; artificial bottom and lack of sediment and organic matters were the main reasons in decreasing of the benthos density. The Chitgar lake is the poorest lake in Iran due to low density of plankton and benthos organisms. The estimation of fish production was 123 kg/ha and for the lake was determaind 16 tonne. The finding display, invasive species was the main fish Chitgar lake that would be negative effect and increase eutriphication trend in the lake. Phosphorus parameter was limited parameters and trophy level recorded low due to high N/P ration in the lake. In overall the Chitgar lake situation is in Oligotrophic category with the low trophy level.
    Keywords: Ecology ; Iran ; Persian Gulf ; Chitgar Lake ; Phytoplankton ; Zooplankton ; Benthose ; Trophy ; Abundance ; Nitrogen ; Temperature
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  • 174
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25821 | 18721 | 2018-10-13 10:32:05 | 25821 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: A mass-balance Ecopath model of coastal areas in the Caspian Sea has been constructed for a quantitative description of the trophic structure of the ecosystem. The model is used to estimate the important biological parameters and relationships among the different ecologically important groups. Twenty five species were used in the present analysis. The model is based on the data collected of Kilka fisheries, beach seining (Bony fish fisheries), and set Gillnet (Sturgeon Fisheries) and also dietary information. Total landings along the Iranian portion of southern Caspian coast reached 39647 t, including sturgeon (41 t), kilka (22873 t) and bony fishes (16733 t) in 2014. Two species of kilka (Common kilka and Anchovy) are important commercially in the Caspian Sea, together accounting in the past decade for 〉 60 % of the total catch, as well as being a crucial part of the food chain. The mean average trophic level was estimated at 3.17 by Ecopath software. In this research, the mean level were studied between eight species varied from 2.56 to 4.04, Sander lucioperca occupy the highest and the as vimba vimba was the lowest level. The ranges of total mortality varied from 0.5 to 2.56 per years. The food consumption rate was estimated about 101.56 per year Mixd trophic level index (MTI) showed small pelagic fishes as prey, have crucial role in feeding of pelagic predators population such as invasion of the ctenophore Mnemiopsis leidyi, bream fish and benthic fish such as sturgeon. In general, niche overlap was greater in more species such as Alburnus chalcoides, Rutilus rutilus, Clupeonella cultriventris, Rutilus kutum and M. leidyi who consumed large amounts of plankton. In conclusion small pelagic fish exert a major control on the trophic dynamics of the Caspian Sea ecosystems and constitute midtrophic-level populations.
    Keywords: Ecology ; Fisheries ; Iran ; Caspian Sea ; Trophic level ; Food consumption ; Total mortality ; Interactions ; Marine ; Food ; Food webs ; Pelagic ; Fish
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25822 | 18721 | 2018-10-13 10:12:48 | 25822 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The Anzali wetland (37.28N – 49.25E) with less than 100 km2 surface area is located in the southwestern shores of the Caspian Sea in the Guilan province. The benthic macro-invertebrates were studied as a part of a main project titled study of aquatic communities variation in the Anzali wetland. The sampling was performed at the 10 stations with using a Van-Veen grab during 2014-2015. The results showed, 14 macro-invertebrates taxa were identified as Chironomidae (Insecta, Diptera) and Tubificidae (Annelida, Oligochaeta), they were dominated in the wetland. The maximum and minimum densities of macro-invertebrates were observed with 609±1119 and 93.32±27.92 n/m^2 respectively at the stations 5 and 1. The silt-clay constitute more than 85 percentage of the wetland sediments with maximum of 93.2±27.92 percentage at the 2 station and minimum 72.21±27.92 at the 5 station. Kruskal Wallis non parametric test (sig. ≤0.05), revealed significant differences for all studied factors at stations while of %tom and grain size had no significant variation. The CCA analysis revealed no accordance of total organic matter (TOM ) and silt- clay percentage vectors with Tubificidae and total benthic frequencies, while Chironomidae frequency is in accordance. Intensifying eutrification process in the Anzali wetland has affected its biota as the benthic fauna are almost limited in to most tolerant taxa which reflect the day after day increasing pollution the Anzali wetland.
    Keywords: Biology ; Ecology ; Iran ; Caspian Sea ; Guilan Province ; Anzali wetland ; Macroinvertebrates ; Eutrification ; Aquatic ; Tubificidae ; Chironomidae ; Insecta ; Diptera ; Annelida ; Oligochaeta ; Benthic fauna
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25825 | 18721 | 2018-10-13 10:43:05 | 25825 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Rainbow trout is the main cultural species of coldwater fishes in iran. Often, aquaculturists intend to breeding in order to production of lines with higher growth rate potential and disease resistant. Nevertheless in the country, no trout breeding programs, has been performed yet and most of the farms focused on the cultivation of the first (unbred) race. While European countries progressed in trout breeding techniques and production lines with higher growth through genetic manipulation (chromosomal number and type changes of fish) and/or selection and their fish products derived from this technology, including eyed eggs and so on have sold to other regions of the world (eg: Iran). In this study, some biological parameters including survival, growth, feed conversion ratio (FCR.) and chromosomal number of two juvenile groups from imported( group 1) and native(group 2) eyed fish eggs were compared. For chromosomal investigation, blood smear test and flow cytometry were performed. sults showed a significant difference (P≤5%) in growth rate of native fishes and French group Native fish feed conversion ratio (0.9) was significantly difference (P≤5%) from that of French fishes (1.15). Chromosomal analysis showed no difference in chromosome number in treatments and two fish groups were 2n chromosome. Based on the results, the fishes of group 1 had faster grow potential and gain weight in less time than that of group 2 and this has been achieved to go through the process of selection and feminization without any change in number of ploidy. Whereas the ability of native fishes in food efficiency ( lower FCR.) was better. However, the reduction of rearing period is the benefit and preference of cultivation of imported or origionally foreign.
    Keywords: Aquaculture ; Biology ; Iran ; Imported eyed egg ; Native fish ; Ploidy level ; Growth ; Rainbow trout ; Chromosome ; Species ; Breeding ; FCR
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25827 | 18721 | 2018-10-13 10:47:16 | 25827 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Induced polyploidy is a suitable tool for producing sterile fish which made commercial benefits in the aquaculture industry. This study carried out in order to produce triploid-interploid population via mating tetraploid female with diploid male rainbow trout. Heat shock was used for making tetraploid population and the best temperature and induction time were examined. Result showed that highest mortality from 1 day after fertilization to emerging were in groups 6 of embryos (40.1%) and the lowest were in groups 3 (33 %). Flowcytometry results showed that some fish were polyploidy. Comparative analyze of genome levels in tetraploid fish to control fish (diploid) and hen as standard indicator, confirmed tetraploid fishes in this study. In conclusion we can state that heat shock induction for 7 minutes at 65 hour –degree after fertilization in 28ºC is optimum temperature for inducting tetraploid rainbow trout.
    Keywords: Aquaculture ; Biology ; Iran ; Sexual development ; Triploid interploid ; Tetraploidy ; Heat shock ; Flow cytometry ; Rainbow trout
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25826 | 18721 | 2018-10-13 10:44:36 | 25826 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Rainbow trout is the main cultural species of coldwater fishes in iran. Often, aquaculturists intend to breeding in order to production of lines with higher growth rate potential and disease resistant. Nevertheless in the country, no trout breeding programs, has been performed yet and most of the farms focused on the cultivation of the first(unbred) race. While European countries progressed in trout breeding techniques and production lines with higher growth through genetic manipulation (chromosomal number and type changes of fish) and/or selection and their fish products derived from this technology, including eyed eggs and so on have sold to other regions of the world(eg: Iran). In this study, some biological parameters including survival, growth, feed conversion ratio (FCR.) and chromosomal number of two juvenile groups from imported( group 1) and native(group 2) eyed fish eggs were compared. For chromosomal investigation, blood smear test and flow cytometry were performed. sults showed a significant difference (P≤5%) in growth rate of native fishes and French group Native fish feed conversion ratio (0.9) was significantly difference (P≤5%) from that of French fishes (1.15). Chromosomal analysis showed no difference in chromosome number in treatments and two fish groups were 2n chromosome. Based on the results, the fishes of group 1 had faster grow potential and gain weight in less time than that of group 2 and this has been achieved to go through the process of selection and feminization without any change in number of ploidy. Whereas the ability of native fishes in food efficiency ( lower FCR.) was better. However, the reduction of rearing period is the benefit and preference of cultivation of imported or origionally foreign.
    Keywords: Aquaculture ; Iran ; Yasoj ; Genetic ; Rainbow trout ; Plan ; Relationship diagram ; Breeding
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25828 | 18721 | 2018-10-13 10:19:08 | 25828 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Different studies on Litopenaeus vannamei (Boone, 1931) has performed in Iran, but less noted in water quality conditions. Water quality, in addition to creating optimal environment for the growth of vannamei shrimp, as well as cause the live food production and improve economic performance are produced. This study was conducted to evaluate the water quality environment vannamei shrimp in the south of Caspian Sea (Mazandaran province) and in the Caspian Ecology Research Center. The results showed that the enrichment of water out of the ponds, can provide favorable conditions for water quality in the culture of vannamei shrimp with different densities. As a result, Mazandaran province environmental conditions (particularly temperature and salinity of the Caspian Sea) has perfectly suited for optimal growth vannamei shrimp. Therefore, vannamei shrimp (PL12) achieved a final individual weight of 21.1gr in a period culture of 85-day with a density of 35 ind/m^2. Also, The nutrients and water quality parameters (Temperature: 27.4±1.79 ° C, Transparency: 10.7±1.2 cm, Salinity: 10.57±0.78 psu, pH: 8.42±0.38, Dissolved Oxygen 8.04±1.35 mg per liter) were within normal limits during the culture period.
    Keywords: Aquaculture ; Ecology ; Environment ; Iran ; Caspian Sea ; Mazandaran Province ; Whiteleg shrimp ; Litopenaeus vannamei ; Salinity ; Physicochemical ; Shrimp
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25829 | 18721 | 2018-10-13 10:21:14 | 25829 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The man-made Lake of the Martyrs of the Persian Gulf (also known as the Chitgar Lake) is located in the northwestern of Tehran and was established in 2012 and field with Kan River water at the same year. The water quality was investigated on 5 sampling site during 2013-2014. Standard methods (APHA, 2005) were used for Analysis of water parameters. The results indicated that the mean monthly temperature of water was 18.4±7.3 °C. The mean monthly EC level was 373±30 µs/cm. The mean monthly total hardness, dissolved oxygen, total phosphorous, total nitrogen, silicate, respectively were 119±5, 7.8±1.2, 0.04±0.01, 2.06±0.41, 9.6±0.5, all as mg/l,. The mean chlorophyll-a was 1.6 ± 0.81 µg/l. All parameters showed no significant differences between stations (P〉0.05), while total hardness, chlorophyll-a, Do, total phosphorus and silicate showed monthly significant differences with 95% confidence level. The ratio of total nitrogen to total phosphorus was 51 which indicated phosphorus is limiting factor in eutrophication process. According to Carlson trophic index determined to be oltraoligotroph (TSI〈40). The result indicated that changes in nutrient concentration is under biological activity influence.
    Keywords: Ecology ; Limnology ; Iran ; Tehran province ; Chitgar Lake ; Water quality ; Martyrs Lake ; Chlorophyll-a ; Phosphorus ; Silicate ; Nutrients
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  • 181
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25830 | 18721 | 2018-10-13 10:49:04 | 25830 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study aimed to assess the social, economic and fish farming in cages in the Southern Caspian Sea. The Caspian Sea is considered as one of the most important water resources of Iran, in which due to the appropriate weather conditions in the north of the country, has the potential for marine aquaculture. With the implementation of this plan would say that with respect to the proposed initial selection from Kheshtsar in Mazandaran province and Kiashahr in Guilan province with along coastline of about 250 km from the depth of 20 to 100 meters and an the area of about 2027km^2 has the cage aquaculture talent. Of course, at the depths of greater than 100 M by using the world technology procedure, there is also the possibility of the establishment of marine cage aquaculture. Now, with the regards of the above potential in the Caspian Sea coastline, it is expected at least 200 cages fish sites farming in with the establishment of 40 cages per site and produce 160,000 tons with creating of 1,000 direct jobs and 2,000 indirect jobs and income 2000 billion gross annual average net profit of 40% could be 800 billion in which will produce of proteins needed part of the country, creating jobs, welfare, prosperity and economy in the region. With the implementation of such national project, it is needed to establish pilot farms to reach the goals: environmental assessment studies, risk assessment, aquatic selection and the use of appropriate cage structures according to ecological conditions of southern Caspian Sea.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Mazandaran Province ; Guilan Province ; Cage ; Economic activity ; Profitability ; Rainbow trout ; Ecological conditions
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  • 182
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25832 | 18721 | 2018-10-13 10:50:13 | 25832 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: A survey on Biodiversity of Cyprinidae family in Iran Cyprinidae family is the biggest and most important fish families with 367 Genera and 3006 species in the world. Members of this family is the most important freshwater species are present in a variety of freshwater ecosystems. Some of them can also live in brackish waters. Iran has about 93 species in the family Cyprinidae and consist half of the fishes in inland waters. In the past two decades many changes occurred in aquatic ecosystems, Fish habitat conditions changed and a lot of them are affected and some species are endangered or put under pressure. Restricted fish migration, Some Exotic species and have been entered in water resources and have narrowed arena on continued existence of native species. On the other hand, climate change and drought on water resources aggravate existing conditions. It is necessary effects of these changes in the family Cyprinidae be reviewed and analyzed.
    Keywords: Biology ; Ecology ; Iran ; Cyprinidae ; Biodiversity ; Water resources ; Climate Changes
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  • 183
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25831 | 18721 | 2018-10-13 10:23:41 | 25831 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Azadegan aquaculture site is located 25 km south of Ahvaz in southern of Khuzestan plain. Primary study of Azadegan aquaculture site was done in 1992 with the aim of developing feasibility of warm water aquaculture and the second phase was done in 1996-1998 by Mohandesin Moshaver Pandam. Now, cyprinus carpio, Hypophthalmichthys nobilis, Hypophthalmichthys molitrix, Ctenopharyngodon idella), Barbus grypus) and Mesopotamichthys sharpeyi rear in this site. Considering special traits of Indian carps such as different level of production in earth pool, diet diversity, and more favorability for consumers, possibility of alternation with common carps, adaptability, growth and maturity in Khuzestan climate, this species can increase the efficiency of Khuzestan aquaculture industry as one of the poles of production in country. In this study the environmental impact assessment of the region was done because of the importance of Shadegan wetland as receiving waste area. Leopold matrix was used for assessment and quantifying environmental impacts of India carp aquaculture. Identification and prediction of effects were done based on the matrix. In using phase, 101 effects were identified which 34 were negative and 67 were positive. 18.8 percentage of effects were physic-chemical, 25.7% was biologic and ecologic and 55.5% was in socio-economic environment. In the operation phase, from all of the anticipated effects in physic-chemical environment 5 effects were positive and 14 effects were negative and in ecologic-biologic environment 17 effects were negative and 9 effects were positive. In socio-economic environment from 56 effects 3 were negative and 53 were positive. In order to summarizing the scores in environmental impact assessment matrix, final scores concluded from weighted scores of different environments including physic- chemical, ecological-biological and socio- economic environments. These scores showed that physical activities will have negative effects due to sewage production and negative effect on water resources. However, because of positive effects in socio-economic and ecologic environments, the final score in +83 that shows general result will be positive especially in economic and social environment. Due to the positive effects of the project on the economic and social conditions such as improving living conditions, creating job opportunities due to culturing this species and changing patterns of employment, increasing income levels at local, provincial and even national scale, increasing the price of land, supporting lateral and related industries, including hatcheries, fish-producing industries, manufacturing and processing industries and conversion, marketing and export, as well as the pharmaceutical industry (veterinary drugs), education development of specialized human resources and its role in the development of aquaculture projects in compliance with the recommendations of management and control is permitted.
    Keywords: Aquaculture ; Environment ; Iran ; Khuzestan province ; Ahvaz ; Impact Assessment ; Indian carp ; Fish pond ; Aquaculture ; Cyprinus carpio ; Hypophthalmichthys nobilis ; Hypophthalmichthys molitrix ; Ctenopharyngodon idella ; Barbus grypus
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  • 184
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25833 | 18721 | 2018-10-13 10:28:58 | 25833 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study aimed to determine domain the adaptability of rainbow trout(Oncorhynchus mykiss) fingerlings in fresh water up to 20 per thousand (grams per liter) for providing facilities for growing this valuable species in the aquatic environment with salinity unconventional been completed. For this purpose the biochemical, bloody and physiologic parameters rainbow trout were studied to determine the adjustment range .Fingerlings fishes from cold water fish farm in the province Mazandran were prepared and for experiments were transferred to the Ecological Institute of Caspian Sea . Fingerlings with an average weight 31.56 ± 0. 07 SE g and average fork length 13.80 ± 0.15 SE cm, in 3 treatments in water with salinities (fresh, 13 and 20 grams per thousand) with a density of 15 numbers in polyethylene to 300-liter tank containing the 250 liters of water testing were introduced. Fresh water from Tajan rivers and water psu13 from the water Caspian Sea and water psu20 by mixing water the Caspian Sea and Sea salt was prepared . The daily amount to 50 percent of the of water tankss been replaced .During the experimental period was for 7 days and were not fed during the experiment.The water parameters was measured during the experiment included 6 ppm dissolved oxygen, pH equal to 8.2 and temperature 15.5 ° C . In the experimental period were not observed Losses in the experimental groups . The results showed that fish gill and kidney introduced in different salinities by making appropriate changes in chloride cells in the gills through increasing the number and the volume of these cells at the base of secondary blades and tubules in the kidney tubules to create greater interior space, are adapted to By changing salinity. Relatively parameters osmolarity, sodium, chloride, magnesium, cortisol, calcium, hematocrit, hemoglobin, number of red blood and white cells in water saltier than freshwater environment was higher (0.05〈 p, Duncan). Changes in hematological and blood plasma ionic parameters and vital organs Fingerlings indicates a willingness adaptability and the ability physiological adaptation fingerling was consistent with changes to environmental salinity brackish water .So, the factors measured with increasing salinity the uptrend that the range of variation for the osmolarite 449-281 mOsmol kg, for sodium, chloride, magnesium, cortisol, respectively, 211- 151, 165121, 3/3 7/0, 87. 53 mmol and the calcium 22-13 mg per dL. The measured values for hematocrit 32.2- 38.8%, hemoglobin 6.2 - 8.6 g per deciliter and the red and white blood cells, was respectively, 1.2-1.7×106 and the 15.6 -18.9×103.
    Keywords: Aquaculture ; Biology ; Iran ; Caspian Sea ; Adaptation ; Rainbow trout ; Gills ; Kidney ; Survey ; Oncorhynchus mykiss ; Fingerlings
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  • 185
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25834 | 18721 | 2018-10-13 10:51:04 | 25834 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Although the most alga blooms usually provide positive impacts on marine ecosystems, but blooming of certain species of algae may also have negative impacts which evidence suggests that over the past few decades the frequency and duration of Harmful Algal Blooms (HABs) have been increasing both nationally and worldwide. Harmful algal blooms of Cochlodinium polykrikoides in the Persian Gulf and Oman Sea were first observed in 2008. In order to provide optimum growth and bloom forming, C. polykrikoides cells were sampled during the bloom conditions in the coastal waters of Bandar Abbass, Qeshm and Hourmoz Islands from March 2012 to June 2015. After sampling, the samples transferred to Persian Gulf and Oman Sea Ecological Research Institute phytoplankton Lab and adapted to filtered seawater. In Phycolab, they isolated and purified by positive phototropism characteristic of species to light. They were grown in modified media culture at different salinities (30, 32 and 35ppt), temperatures (20, 23, 26 and 28ºC) and intensities (35, 70 and 90 µmol m^-2s^-1). During this study 3 Dinoflagellates species were identified in Hormozgan Coastal waters. The first species was Noctiluca scintillans. This species was alive in F/4 media culture and under the 32ppt salinity, 25°C temperature, and an 11h light: 13h dark photoperiod regime only for 4 months. The second species was Protoperidinium quinquecorne and produced temporal blooms that could not be isolated under usual and modified media cultures. The last Dinoflagellates species that caused spreading blooms in Hormozgan Coastal waters and could be possible to isolate was Cochlodinium polykrikoides. The results clearly showed that the best media culture for growth of this species is A2 and the highest alga biomass was obtained following culture under the 32ppt salinity, 26°C temperature, and under an 11h light: 13h dark photoperiod regime at a light intensity of 90 µmol m^-2s^-1 provided by cool white fluorescent tubes. Maximum cell density of C. polykrikoides in a 5 liter Erlenmeyer for 12 days reached to 1.6 × 106 cell L^−1 with 2-12 and occasionally to 16 cells chain. Based on the results from the present study, providing suitable media culture and physical condition, bloom forming of C. polykrikoides start from day 8 and will be continued until day 24.
    Keywords: Aquaculture ; Health ; Iran ; Persian Gulf ; Harmful algal bloom ; Isolation ; Dinoflagellates ; Growth ; Cochlodinium polykrikoides
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25835 | 18721 | 2018-10-13 10:49:48 | 25835 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was conducted for investigation on algae bloom monitoring and oscillations of biotic and non-biotic parameters in coastal waters of Bandar Abbas from October 2013 to Septembre 2014. In this project, planktonic distribution and environmental data were collected from five stations, every 10 days. Four order and 45 genus of phytoplankton (Bacilliarophycea 29 genous, Dinophycea 13 genus, Cyanophycea 2 genus, Raphidophycea 1 genus) were identified, as well as , 10 order of zooplanckton consisted of Copepoda, Mollusca, Chaetognatha, Sarcomastigophora, Urochordata, Annalida, Nematoda, Echinodermata, Ciliophora and Granuloretica. Results showed that Bacillariophycea were dominate among phytoplankton species in all season. So that, Leptocylindrus, Chaetoceros and Nitzschia from Bacillarriophycea, Protoperidinium and Gymnodinium belong to Dinophycea, and Oscillatoria belong to Cyanophycea were dominate genus in Bandar Abbas coastal waters. The maximum and minimum density and frequency for phytoplankton were recorded respectively from Shilat Jety and Airport Stations. Based on ANOVA analysis on phytoplankton density no significant were observed seasonally (p〉0.05), but significant differences were observed between stations (p〈0.05). Algal blooms were recorded for Protoperidinum, Chaetoceros and Noctiluca form Shilat Jety in the study period. Result showed that Crustacea have more density amongst zooplankton community, although Arthropoda were dominate in all season. The maximum frequencies were recorded for Nauplius (78%) and Copepoda (21%), also Among copepod, Calanoida order observed 68.5% frequency in the study period. The ANOVA analysis showed significant differences both in stations and seasons for zooplankton density (p〈0.05). The seasonal range of temperature were recorded between (19.43±2.58 - 31.98±2.48 ), salinity (27.15±9.48 - 38.13± 0.72ppt), dissolved Oxygen (2.8±1.81-6.87±1.25) mg/L, and pH (8.06±0.49- 8.69±0.23). Evaluation of biotic and abiotic factors indicate the probability of unhealthy and unstable Shilat jety and Gorzoozan stations coastal waters of Bandar Abbas City. One of these cases can be mentioned to high density of cyanophyceae in the estuary Gorzoozan compared other stations , high density of Phytoplankton and low diversity in the Shilat station. While the increase in Harpacticoida order of copepods As an indicator of pollution than on previous reports of other researchers In the this area, suggests pollution is pushed into the area.
    Keywords: Ecology ; Iran ; Persian Gulf ; Bandar Abbas ; Environmental parameters ; Algal bloom ; Phytoplankton ; Zooplankton ; Survey ; Planktonic communities
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25836 | 18721 | 2018-10-13 10:31:51 | 25836 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The most important habitats of mudskippers are muddy areas in tidal zone of tropical mangrove forests. Mudskippers are related to Oxudercinae subfamily of Gobiid fishes. Three most distributed species of Hormozgan mudskippers were Periophthalmus waltoni, Boleophthalmus dussumieri and Scartelaos tenuis. These fishes can be considered as euryhaline and eurythermal aquatic species, because they can tolerate a wide range of salinity and temperature. A research was done since september 2008 to september 2009 in two important mangrove regions of Hormuzgan (Tyab and Khamir) to determine some ecological characteristics of inhabited mudskipper species. Results showed that nitrate levels are significantly different between tidal lines and seasons (P〈0.05). Maximum nitrite concentrations were recorded 53.2 and 92.5 µg/l in Khamir and Tyab respectively. The annual correlation matrix showed that a positive correlation between phosphate concentration and nitrite and silicate (P〈0.05). Silicate concentration was very high, because of too low density of diatoms and radiolarians. Some species of diatoms, dinoflagellates, cyanobacteria and larvae of crustacea and echinoderms were observed with different density and diversity. Sediment composition of the studied areas were categorized in three classes (clay, sand and clay - sand). Polychaetes formed dominant group of benthic fauna in Tyab and Khamir areas. High density of capitellid worms was possibly related to some environmntal stress caused by activity of fishing and cargo vessels. It was not observed significant difference between fishes length in two areas (P〈0.05); Mean lengths of P. waltoni, B. dussumieri and S. tenuis were calculated 9.85, 14.7 and 11.5 cm respectively. Spawning period of each three species in both areas were obtained from late winter to late spring based on gonadosomatic index values. Male to female sex ratio of P. waltoni, B. dussumieri and S. tenuis were calculated 1:0.45, 1:0.41and 1:0.74 respectively. Absolute fecundity of P. waltoni, B. dussumieri and S. tenuis were estimated 3558 ± 2202, 3952 ± 1030 and 6742 ± 1939 respectively. P. waltoni feeds mainly on fiddler crab, S. tenuis uses crustaceans and gastropods and B. dussumieri has a vegetarian diet.
    Keywords: Ecology ; Iran ; Persian Gulf ; Hormozgan Province ; Mudskippers ; Ecology ; Periophthalmus waltoni ; Boleophthalmus dussumieri ; Scartelaos tenuis ; Female ; Male ; Benthic fauna
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25839 | 18721 | 2018-10-13 10:48:08 | 25839 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The aim of this project is to study water quality and the trophic status at different months and depths in the southeast Caspian Sea-Goharbaran based on environmental parameters and nutrients during 2013-2014 in order the feasibility of fish cage culture. Results showed that water temperature, transparency, pH, dissolved oxygen, BOD5, COD, total alkalinity and TSS were varied 9.00-29.00C, 0.50-12.00 m, 8.05-8.74, 5.76-12.85, 14-101, 21-195 and 0.00-0.12 mg/l at the different months and depths, respectively. In addition, the values of NH^4+, NH_3, NO^2-, NO^3- and PO^4-3 were 0.007-0.051, 0.001-0.010, 0.002-0.015, 0.043-0.477 and 0.014-0.077 mg/l, respectively. Chl-a concentration was recorded as 0.060-8.02 mg/l. Scaled and unscaled trophic indices were equal to 3.42-5.52 and 2.61-5.85, respectively. The proper temperature for cultivation of salmonids species in this area began from October to March. The range of the standard of pH and dissolved oxygen level for fish farming cage were 7.80-8.50 and 〉5 mg /l that in current study results of pH and dissolved oxygen which were consistent in the current study. Various forms of nitrogen (ammonium, nitrite and nitrate ) at the different layers were acceptable and less than the threshold limit of Australia and New Zealand. The results of the nutrients concentrations compared with data from the reference years (1996-1996 ) in the Caspian Sea (Goharbaran region) showed that this ecosystem passed from oligotrophic status and shifted to mezotrophic and eutrophic condition. As a conclusion, although nutrients concentrations were acceptable in terms of fish farming cages in this region, some parameters such as chemical oxygen demand and transparency are not suitable for aquaculture industry. Meanwhile, based on various trophic levels, this ecosystem (Gohrabaran region) has a mezotrophic status with a high risk of eutrophication.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Goharbaran ; Physico-chemical parameters ; Water ; Fish cage culture ; Temperature ; Transparency ; pH ; Dissolved oxygen
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  • 189
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25838 | 18721 | 2018-10-13 10:39:41 | 25838 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Region South East of the Caspian Sea (area Goharbaran) having valuable resources biological flora and fauna, the diversity of commercial fishes, especially reserves the exclusive sturgeon as well as reserves abiotic such as oil and gas resources and transit of goods through Bandar Amirabad to Central Asia , the ecological conditions for the implementation of the project is very necessary. The aim of this study was to determine species composition, spatial and temporal distribution of plankton and fish of this region. Plankton sampled from different depths (5, 10 and 15 m) were carried out. Sampling of phytoplankton did by Ruttner and sampling of zooplankton did by net with mesh size 100 microns. The fish were sampled monthly from December 2013 to July 2014 and within months was carried out. From livestock gill Monofilament.. In this study, a total of 130 species of phytoplankton of 7 filums Bacillariophyta (60 species), Pyrrophyta (23 species), Cyanophyta (22 species), Chlorophyta (14 species), Euglnophyta (9 species), Haptophyta (1 species) and Chrysophyta (1 species )and 24 species of zooplankton branch of Copepoda (5 species), Rotatoria (7 species), porotozoa (3 species), Cladocera (9 species), and Meroplankton (2 species) of larvae of Cypris Balanus and bivalves Lamellibranchiate larvae and 256 fish Acipenser persicus at 5, Alosa braschnikowi 71 number, Alosa caspia 40 number, Benthophilius lipidus 1 number, Cluponella cultriventris 72 number, Cyprinus carpio 1 number, Liza saliens 15 number, Neogobius bathybius 1 number, Neogobius caspia 5 pcs, Neogobius flauviatilis 19 number, Neogobius gorlab 6 number, Rutilus kutum 14 number, Rutilus rutilus 2 pcs, vimba vimba 4 number was observed.Different ecological conditions appointment dietary needs and relationships of organisms and their adaptations to the environment, the density and distribution of different species of phytoplankton, zooplankton and fish specifies.Also the Caspian Sea due to the type of biological species and number of endemic species (42%) in addition to comb jelly invasion, were force of the effects some species like Gloeotrichia Echinulata and as a result, now or in the future, more species will be observed and recognized will be of most interest. Also Psedonitzschia seriata ability to produce Domick acid that can be hazardous to aquatic animals and even human, was in Goharbaran area. This was considered for fishes of the southern Caspian Sea and ecological distribution of most species depends on the region. The abundance of two species of whitefish and pelagic fish in Ghahrebaran region is more than the whole Caspian Sea. On the other hand, the dominant phytoplankton of this region is Bacillariophyta and dominant zooplankton is Copepoda, which shows the goodness of these plankton branches, and by changing the various factors as the different terms of receiving solar energy and resulting in temperature and water currents can cause seasonal differences in the density of the Bacillariophyta branch and also the Copepoda, therefore one of the most important factors is season, and in the winter, when the aquatic rotation of this ecosystem increases, it increases the nutrients and moves it from the floor to the water column, and as a result, increasing the amount of silica in various levels of water can affect the nutrition of fish.
    Keywords: Ecology ; Iran ; Caspian Sea ; Mazandaran Province ; Goharbaran ; Plankton ; Diversity ; Abundance ; Biomass ; Pelagic fishes
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25845 | 18721 | 2018-10-13 08:35:24 | 25845 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Biological characteristics of Liza klunzingeri were studied in two coastal areas, Sajaphi and Bahrekan, of eastern Khuzestan during March to February 2007. Among total 1880 measured fish specimens, 947 specimens were analyzed. The mean value of Gonado-somatic Index (GSI) for the male and female fish were calculated as 0.96± 1.39 and 3.25 ± 3.26 respectively. The GSI value was highest in November and lowest in July. The mean value of condition factor (K) was 1.25± 0.14 in male and 1.21± 0.15 for female. The highest K value were observed in June and the lowest value in February. The lenght at first maturity regardless of sexuality, was found to be 14.5 cm and the time of spawning based on reproduction pattern were determined in Nov- Dec. The length-weight relationship were calculated as Y=0.024L^2.76 (n=226R2=0.72) for males, Y=0.011L^3.00 (n=444R2= 0.78) for females and Y=0.0208L^2.82 (n=670R2 =0.82) for total fishes and also it’s found significant in level length weight relationship in (P〈0.05). According to biological characteristics and referring to American fisheries society (AFS) indices and Fuzzy logic expert system, Lize klunzingeri is classified as low vulnerable species.
    Keywords: Biology ; Iran ; Khuzestan province ; Sajaphi ; Bahrekan ; Lize klunzinger ; Gonado-somatic Index ; GSI ; Condition factor ; Biological characteristics ; Female ; Male ; Specimens ; Fisheries
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    Iranian Fisheries Science Research Institute
    In:  http://aquaticcommons.org/id/eprint/21097 | 17408 | 2017-04-27 06:20:06 | 21097 | Islamic Azad University, Science and Research Branch, Tehran
    Publication Date: 2021-07-14
    Description: Thalassoma lunare is also named the Moon Wrasse because of its yellow caudal fin shapes like crescent moon with long upper and lower lobes. It is an inhabitant of coral reefs and surrounding areas at depths from 1 to 20. T. lunare is the most abundant wrasse species in the Persian Gulf. This species can reach 16 cm in total length in Iran, Persian Gulf, Kish Island. Moon wrasses are active fish, said to be moving all day long. They are also territorial, nipping, chasing, and otherwise harassing fish that get in their way. Being diurnal, wrasses have strong vision, although they also have a decent sense of smell. At night, they rest in niches often under rocks or other such structures. If needed, a moon wrasse may dig out a space under a rock by repeatedly swimming through it until it fits without struggle. They are protogynous hermaphrodites, all starting off as females and changing to males, a process which, for the moon wrasse, takes only 10 days. Some moon wrasses live in groups consisted of a dominant male, and a "harem" of about a dozen other wrasses, some female and some male. The alpha male is more brightly colored, and at every low tide hour, changes from green to blue, and goes into a show of attacking and nipping all the other wrasses. This is his way of showing his dominance to the rest of the males and keeping the females in check. During breeding season and before high tide, the alpha male turns completely blue, gathers up every single female, and the spawning frenzy begins.
    Keywords: Biology ; Ecology ; Fisheries ; Thalassoma lunare
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  • 192
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    Iranian Fisheries Science Research Institute
    In:  http://aquaticcommons.org/id/eprint/21105 | 17408 | 2017-04-27 07:28:58 | 21105 | Islamic Azad University, Science and Research Branch, Tehran
    Publication Date: 2021-07-14
    Description: Megalomma vesiculosum (Giant Feather Duster Worm) has reported for the first time from Persian Gulf (Iran, Kish Island). M. vesiculosum is a polychaete worm that reaches up to 12 cm in length. It has relatively few (about 50) tentacles, each with a large eyespot at the free end. It creates a long and tough tube, which is mostly encrusted untidily with large shell fragments and small stones. The tube protudes no more than 40-50 mm (in Iran, Kish Island) from coarse sand or muddy gravel at low water and below. The body and crown vary in color from yellowish to a reddish-purple brown and is usually spotted. The Giant Feather Duster Worm is a relative of the garden earthworm. It uses its colorful tentacles to filter the water for food, which includes phytoplankton and bacteria. M. vesiculosum requires a high density of phytoplankton and dissolved particulates to survive in a marine ecosystem. The worm generally buries its tube in the sand and the crown protrudes above to strain the water. If this worm sheds its tentacle crown, it is not getting enough food. It will usually regrow another, smaller than the previous. If this cycle continues, the worm will starve. Members of the class Polychaeta are mostly gonochoric (sexual). Females produce a pheromone attracting and signalling the males to shed sperm which in turn stimulates females to shed eggs, this behavior is known as swarming. Gametes are spawned through the metanephridia or body wall rupturing (termed as ‘epitoky’, wherein a pelagic, reproductive individual, ‘epitoke’, is formed from a benthic, non reproductive individual, ‘atoke’). After fertilization, most eggs become planktonic; although some are retained in the worm tubes or burrowed in jelly masses attached to the tubes (egg brooders). Eggs develop into trocophore larva, which later metamorph into juvenile stage (body lengthened), and later develop into adults.
    Keywords: Biology ; Fisheries ; Megalomma vesiculosum
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  • 193
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    Iranian Fisheries Science Research Institute
    In:  http://aquaticcommons.org/id/eprint/21106 | 17408 | 2017-04-27 06:38:05 | 21106 | Islamic Azad University, Science and Research Branch, Tehran
    Publication Date: 2021-07-14
    Description: Sabellastarte spectabilis is commonly known as the feather duster worm, feather duster or fan worm. It has reported for the first time from Iranian waters (Kish Island) and finding in the intertidal and subtidal reefs. Especially common in sites where phytoplankton is abundant. The worm's body occupies a flexible mucus tube formed by adhesion of silt from the water column. Polychaetes, or marine bristle worms, have elongated bodies divided into many segments. Each segment may bear setae (bristles) and parapodia (paddle-like appendages). Some species live freely, either swimming, crawling or burrowing, and these are known as "errant". Others live permanently in tubes, either calcareous or parchment-like, and these are known as "sedentary". This large worm can reach 80 millimeters in length and 10–12 millimeters in width. It is buff in color with purple specks. It lives in a tough, leathery tube covered with fine mud. The tentacles are striped in dark and pale brown bands and bear neither stylodes nor eye spots. There are two long, slender palps and a four-lobed collar. Cilia on the tentacles cause currents in the water and organic particles are caught as they float past. They are channelled along mucus-filled grooves to the mouth. Larger non-food particles are used for building the tube. The tentacles are also used as gills for gas exchange. This worm can reproduce asexually by fragmentation, and can regenerate body parts after being damaged. Reproduction can also be by sexual means. Most worms are either male or female and the gametes mature in the coelom before being released into the water column. Some specimens, particularly larger ones, have both male and female gametes and a study concluded that this was consistent with sequential hermaphroditism. Fertilization is external, and after a short time in the plankton, the trochophore larvae settle out and grow into adult worms.
    Keywords: Biology ; Fisheries ; Sabellastarte spectabilis
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  • 194
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    Iranian Fisheries Science Research Institute
    In:  http://aquaticcommons.org/id/eprint/21107 | 17408 | 2017-04-27 06:39:06 | 21107 | Islamic Azad University, Science and Research Branch, Tehran
    Publication Date: 2021-07-14
    Description: Pseudoceros prudhoei is usually found on rocky or reef habitats, swimming in open waters or under coral rubble plates, where they find their prey, the ascidians. Maximum length is 5 cm in Persian Gulf (Iran, Kish Island). While some of the free-living flatworms tend to hide under rocks escaping from the sunlight, colorful polyclads usually demonstrate a very conspicuous behavior, swimming through the water column next to coral reefs. These animals actually do not need to avoid predators, because their bright color pattern works as a warning sign about their distastefulness and toxicity. None of the animals had a negative response to the presence of light. When the flashlight was turned on, they started swimming around and exploring the environment and none of the worms hid under the rocks searching for cover. In fact, the light didn’t seem to bother them at all, as some would swim toward it, swim back and settle down at any spot. P. prudhoei is not negatively phototactic and reinforce the function of aposematic coloration in pseudocerotids. P. prudhoei is hermaphrodites with both male and female reproductive systems functional at the same time, and reproduce by internal fertilization.
    Keywords: Biology ; Fisheries ; Pseudoceros prudhoei
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  • 195
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25079 | 18721 | 2018-08-22 06:43:41 | 25079 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was carried out from 2001 to 2002 in Gamasyab River of Hamadan province of Iran in order to identification of indigenous species of this water resource. The main objective of this study was to fish identification for scientific purposes, geographical distribution pattern, preparation atlas of the fishes, introduction of commercial species and awareness on ecological situation of their natural habitats. Also providing necessary information for conservation and sustainable use of these valuable stocks and minimizing environmental impacts of unsecured utilization. Sampling were carried out by selecting 15 sampling stations through the river and fishing by electro fisher and cast net. Identification accomplished by valid identification keys in species level. Photography and slides provides and 10 pieces of each species tagged by unique codes for preservation in national museum. 50 morphometric and meristic characteristics studied for scientific identification.. Indigenous fishes belong to 14 species, 10 genus, 3 family and 2 order. Indeed 2 exotic species belong to Cyprinidae and Salmonidae families recognized. From these indigenous species, 11 belong to Cyprinidae family and only one species belong to Sisoridae family.
    Keywords: Biology ; Iran ; Hamadan ; Gamasyab River ; Indigenous species ; Identification ; Fish ; Sampling ; Morphometric ; Cyprinidae ; Salmonidae ; Sisoridae
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  • 196
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25083 | 18721 | 2018-08-22 07:15:57 | 25083 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Mullet and other bony fishes are caught by beach seine in Iranian waters in Golestan, Mazandaran and Guilan provinces. In this investigation which had conducted in commercial regions during 2003-2004, we estimated catch and CPUE, species composition, length, weight, age of two species of mullet and Salmo trutta caspius; von Bertalanffy growth parameters, mortality, biomass and MSY of Liza aurata. The total recorded catch of mullet was 3870 mt and L. auratapre dominated (98.5% of total catch of mullet). CPUE of two species of mullet; L. aurata and L. salienswere 69.6 and 1.1 mt purse seine, respectively. The catch of Salmo trutta caspius was negligible (about 3.6 mt). Biomass and MSY (maximum sustainable yield) of L. aurata were estimated to be 11019.8 and 3322.6 mt, respectively.
    Keywords: Fisheries ; Iran ; Golestan Province ; Mazandaran Province ; Guilan Province ; Mullet ; Bony Fish ; CPUE ; Species ; Length ; Weight ; Age ; Salmo trutta caspius ; Von Bertalanffy ; Mortality ; Biomass ; Liza aurata ; L.aurata
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25088 | 18721 | 2018-08-22 06:46:47 | 25088 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: One of the effective factors in cyst production on Artemia culture ponds is the number of oviparous females and density of biomass in view of adult's weight per liter of ponds water. In this study, the effect of oviparous female's abundance (Reproductive Females lit^-1) on daily cyst yields with using the ANOVA and correlation Analytical method were assayed. The result indicated that, with presence the small number of oviparous females (less than five Ind lit^-1) and Artemia density (between 0/1 to 0/2g/ lit) on culture ponds, the cyst yields at first sixty days culture period (160 Kg.dw/ha) were more than yields that harvested at three months later (47 Kg.dw/ha) and on the second three months of cultural period despite of presence the larger number of oviparous females (more than twenty Ind/lit) and presence the same Artemia population density, the daily cyst yields was declined.(Sig=0.000).
    Keywords: Biology ; Iran ; Artemia ; Density ; Cyst ; Fertilized pond ; Females ; Biomass ; Weight ; ANOVA
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  • 198
    Publication Date: 2021-07-16
    Description: Mnemiopsis leidyi is one the species of comb-jelly. It belongs to the Ctenophore phylum and Lobate order. It is a purely marine, free-swimming animal with a transparent jelly-like walnut-shaped body, which was transported from the Black Sea into Caspian possibly at the end of 1990s. This species is food rival of pelagic fish. It has been reported that the ctenophore caused the dramatic decrease of zooplankton and pelagic fish stock in the Black Sea. In this study, sampling on Mnemiopsis leidyi, zooplankton, phytoplankton was conducted from December 2003 to March 2005, from a total of 11 stations located along four transects (Anzali, Khazarabad, Tourkman and Gorgan Bay) by METU net in the Iranian coasts of the Caspian Sea. Clupeonella catch was done by light fishing with fishing vessel in Anzali region. The results showed that the biomass of M.leidyi had some fluctuation in different seasons and its maximum biomass was recorded in summer with a figure of 524.4±156.2 g.m^2 Tourkman region and the minimum biomass was observed in winter with a figure of 5.15±1.81 g.m^2 in Anzali region. Study of stomach of Mnemiopsis showed, that Acartia (belonged of Copepoda with 66 %), lamlibranchia (13 %) has the highest frequency and the lowest were Balanus nauplii, Rotatoria, Tintinnopsis and Podon polyphemoides. The maximum mean abundance of zooplankton was 29368±24318 n.m3 in winter (Anzali region) and the minimum was recorded 4170±5014 n.m3 in summer (Tourkman region). Copepoda was dominant zooplankton in the Iranian coasts of the Caspian Sea. Chrysophyta with 150000000±440000000 n.m3 and Euglenophyta with 260000±521000 n.m3 had the highest and the lowest frequency respectively in the Caspian Sea. The main foods of Clupeonella were Acartia with 80 %. The survey of stomach of Clupeonella showed that the feeding of these species was not good and the highest fullness index was recorded 220.95±314.67. These results showed, the main food item of Ctenophora and Clupeonella were Copepoda (Acartia). It seems, the impact of Mnemiopsis leidyi feeding has been the important factor in declining zooplankton populations, Clupeonella stocks, and also increasing of phytoplankton abundance.
    Keywords: Biology ; Iran ; Caspian Sea ; Gorgan Bay ; Bandar Tourkman ; Khazarabad ; Anzali ; Ctenophora ; Mnemiopsis leidyi ; Diet ; Species ; Phylum ; Pelagic fishes ; Sampling ; Zooplankton ; Phytoplankton ; Acartia ; Ctenophora ; Population ; Abundance
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  • 199
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25254 | 18721 | 2018-09-07 07:13:59 | 25254 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Present study carried out in 6 earthen ponds (3500 m^2 each) at the Zahak fish propagation and cultivation center. In July 2005 ponds were prepared and stocked with 1100 fish specimens with 40 g average weight. Stocking normative in two ponds (1 and 4) were 220 common carp, 110 grass carp, 715 silver carp, and 55 big head specimens. In the next two ponds (5 and 6) common carp reduced to 50% and Shizotorax zarudnyi was replaced. In the rest two ponds (7 and 8) common carp reduced to zero and totally replaced with Shizotorax zarudnyi. Manure and inorganic fertilizer were used during cultivation period. Carp was fed by concentrated food and grass carp was fed by alfalfa. At the end of the cultivation period (16 months) mean weights of common carp, grass carp, silver carp, big head, and Shizotorax zarudnyi reached 1201 g, 1572 g, 313 g, 486 g, 531 g respectively. Mean daily growth were 2.41 g, 3.19 g, 0.56 g, 0.92 g, 1 g respectively. Survival rate during cultivation period were 97.57%, 68.3%, 94%, 85.45%, 46.85% respectively. Biological parameters were recorded once a month. Other chemical and physical parameters such as air and water temperatures, oxygen, pH, twice per day and factors such as nitrite, nitrate, Ammoniac, transparency, salinity and phosphate were measured and recorded once per week. During cultivation period average pH was 7-10.34, water temperature was 0.5-33 °C, transparency was 12-115cm, salinity was 1.1-5 mg/l, oxygen was 2-10 mg/l, nitrite was 0.86-0.16 mg/l, nitrate was 0.06- 2.21 mg/l, and phosphate was 0.1-1.8 mg/l. Average total yields in the ponds 1 and 4 with standard normative for the region were 554, and 623 kg. In the ponds 5 and 6 with 50% Shizotorax zarudnyi were 514 and 518 kg, and in the ponds 7 and 8 with total Shizotorax zarudnyi replacement were 533 and 386 kg.
    Keywords: Aquaculture ; Iran ; Sistan region ; Fish culture ; Poly-culture ; Shizotorax zarudnyi ; Earthen ponds ; Specimen ; Common carp ; Grass carp ; Silver Carp ; Fertilizer ; Survival rate ; Transparency ; Salinity ; Temperature ; Nitrite ; Nitrate ; Ammoniac ; Tehran ; Iran
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25258 | 18721 | 2018-09-07 07:37:38 | 25258 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Neogobius caspius is a small benthic fish that is native to the Caspian Sea. The importance of this fish is because of it is role as a main food resource of the Sturgeon fish. The genetic diversity of N.caspius populations in the Caspian Sea was studied using microsatellite technique. In the study 95 Samples of Neogobius caspius from two regions (Torkman port and Chalus) in south Caspian Sea were collected. DNA was extracted using 12 pairs of microstellite primers polymerase chain Reaction (PCR) was conducted. DNA Bands were analyzed using uvdoct and GenAlEx software package. Out of 12 microsatellite primers, 11 loci were produced, in which 5 of them were polymorphic, 6 monomorph and one of them showed smear. Average of observed and expected hetrozygosity was 0.798 and 0/843 respectively. Significant genetic differences between 2 regions was observed (P〈0.01). Deviations from hardy- Weinberg equilibrium were in all cases. These results indicate that at least, 2 population of Neogobius caspius exist in the south Caspian Sea.
    Keywords: Biology ; Iran ; Caspian Sea ; Torkman port ; Chalus ; Microsatellite ; Genetic ; Population ; Neogobius caspius ; Benthic ; Native ; Sturgeon ; Diversity ; Samples ; PCR
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    Type: monograph
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