ALBERT

Description: Qhar-khetlu dam reservoir is in the central part of Ijrud city of Zanjan province with a reservoir volume of 500000 and a water volume of 900000 cubic meters, covering an area of 6 hectares and containing 3500 hectares of the watershed in order to control seasonal floods, drinking water in the village, strengthening the aquifers of the area and As a water supply in the dry seasons, 120 hectares of agricultural land was planted for irrigation.Study of this water source with the aim of measuring biological and non-biological factors, determination of aquaculture production and Fish release capacity and fishing capacity for optimal use of fishery management in Zanjan province was done in 2006.The results of hydrochemical analysis of the water of Qharkhetlu dam reservoir showed that the minimum and maximum temperature of water ranged from 4.5 to 26 ° C, the pH of the water was 4.7 to 8.8, the dissolved oxygen was 7.7 to 12.2 mg, the total water hardness fluctuation was 154 194 mg / L and electrical conductivity of 272 to 390 micrometers per square centimeter, the water transparency was 25 to 380 centimeters.In this study, six phytoplankton classes with 31 genera and 3 classes of zooplankton with 14 genera were obtained. The abundance of phytoplanktons was counted from 150,000 to 206,000 per liter, and the average chlorophyll a, 1.10 micrograms, was estimated. The frequency of zooplanktons were 32 to 132 per liter, as well as the mean of 544 per square meter of macrobenthos.By comparing of the physical, chemical and biological factors and also the results of previous studies of this water source, the conditions for the introduction of warm water and cold water fishes including silver carp, big head carp, rainbow trout out and native fishes including species of Barbus and Capoeta genera are suitable. Estimation of Qhar-khetlu dam reservoir production is 80 kg / ha and its production capacity is 484 kg per year.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Caspian trout (Salmo trutta caspius) is one of valuable fishes of the Caspian sea for people living in the Northern coastal area of Iran. Since fish stock and natural resources protection is one the Iranian fisheries research objectives, the most activity on Caspian trout rehabilitation was focused on releasing thousands of smolts in the rivers discharge to Caspian Sea. Catch statistics of this species has declined over last decades thus this study on quality of provided brood stocks and producing fries with suitable quality can help rehabilitation and rearing of this valuable species. Several rivers were considered for providing brooders to keep genetic resources variety of sea stocks. In spawning season 25 (9 male, 16 female) and 30 (5 male, 25 female) brooders were collected from fishermen in Mazandaran rivers and Gyilan coasts provinces respectively. After propagation and larvae rearing, 11500 (10-15 g.) and 50000 (less than 10 g.) juveniles were released in Gyilan and Mazandaran rivers respectively.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: A sharp decline in natural population of Caspian brown trout lead to implementing of restocking program in Iran. Along the stock recovery program, the artificial production of this species has been attracted too much attention in recent years. The objective of the study is to assess the effects of protein and energy levels of diet on fish performance, growth efficiency of Caspian brown trout in order to develop a proper diet for production of this species. Six experimental diets were formulated by replacing casein as a pure protein source with fat as an energy source in the diets according to a 3×2 factorial design. The diet was assigned to 18 tanks with 50 fish each, with three replicated for each diet. The experiment was conducted for 8-weeks with Caspian brown trout with an initial body weight of around 7 g. Caspian brown trout demonstrated a better FCR and a larger SGR at lower protein levels (45 and 50%) in comparison to high protein level (55%; P〈0.05). Protein efficiency ratio improved by a decrease in protein content of the diet (P〈0.05), but energy content of diets does not affect any growth related parameter such as FCR, SGR and PER. There was also no interaction between protein and energy levels in the growth related parameters, suggesting the effect of protein on the growth parameters in Caspian brown trout did not depend on energy levels of diet. In conclusion diet containing 50% protein can support the maximum growth. High dietary energy level of the diet dose in cooperate in protein sparing effect.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In order to domesticate Litopenaeus vannamei as a solution to single species shrimp aquaculture of Penaeus indicus and to reduce risk of shrimp culture in emergency cases, this study was carried out. Initially the postlarvae reared from imported L. vannamei were cultured. After culture season the adult shrimps were transported to greenhouse ponds for wintering. It is then relocated to hatchery saloon. After eye stalk ablation and maturation the female brood stocks were introduced to matured male for copulation at ponds. During the study period, male and female shrimps attained full maturation at end – April by temperature over 25ºC, the copulation process was started significantly. Finally of the 12 instances of copulation, one instance resulted nauplii production. The best maturation was observed at 28 ֯C ±1 and salinity30 to 33PPT and pH of 8 to 8.3 Fresh sea worms and squid showed the highest effect on fecundity in brood stocks. Besides, the spring season was obtained to be the best period for brood stock production in Bushehr province.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Following to introduce Vannamei shrimp (Litopenaeus vannamei) into Iran aquaculture industries by fisheries organization its postlarvae were successfully reared in ponds in Bushehr and Choebdeh in Khuzestan. Due to activity of Shrimp site and importance of input and output water quality, this study was done in 11 stations including: two stations in Bahmanshir river, two station in C4 and C5 irrigation canals, six staions in active farms along C4 cannel (Yonesi, Mosavei and Salman Zadeh), and C5 cannel (Mohamadi, Khairi and Ashraf Por) and one staion in output draing were selected. Sampling were carried out from May2009 befor beginning stocking to harvest time in October 2009. Physico- chemical parameters and plankton sampled biweekly and benthic animals and heavy metals were sampled monthly. Some parameters such as pH and temperature by using portable multy parameters and others. Analyzed with standard methods. Heavy metals were measured by plarography method (797 Vamtro). Plankton and benthos samples were studied only in Bahmanshir station. According to results the maximum values for DO (11.1 ppm) PO4 (1.86 ppm) NO3 (8.4 ppm) and TSS (4992 ppm), pH (8.4) NO2 (0.18 ppm) BOD5 (9.06 ppm) were measured. In comparison to aquaculture water quality standards except for TSS in river stations others are located in allowable range. We didn’t observed any main changes in water quality from river to output drainage. The results of biotic parameters in river stations showed that diatoms were the most group of phytoplanktons. Zooplankton groups were Copepods, Rotifera and Protozoa. Tintinids and Nematoda larvae were the most abundant zooplankton. Benthic animals were included nine crustacean species and two annelid species. Isopods with 52% were the most and ant group.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Carp aquaculture is one of Maine potential for commercial and rural development , employing enhancement and earning income .This enterprise which is conducted by using fresh water and unfertile lands have been attended in the country since last seven decades. In accordance to wide area of cultivation in the country, maximum production is about 150000 tonnes in 2010. Establishment of aquaculture center by technical deputy of president, made a suitable opportunity for preparation of carp development road map. In this report, present condition and problems in front of carp development have been analyzed , and regarding to targets of long term country development, projects for research and executive sectors have been prepared.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The brine shrimp Artemia consists of a number of bisexual species and a large number of parthenogenetic forms, which collectively, inhabiting a wide range of hypersaline habitats. This research was carried out in order to study the genetic differentiation of Artemia urmiana and two parthenogenetic populations originated from surrounding lagoons of the Lake Urmia (Fesendoz, Saray village) and also to find the phylogenetic relationships of some Iranian Artemia (Qom, Arak, Golestan, and Shiraz) with a comparison to some foreign Artemia species (A. sinica, A. franciscana, A. parthenogenetica (Russia and Turkmenistan) using a molecular technique (PCRRAPD`s). Of a total number of 56 random primers (10 bp) survey, 29 and 26 oligonucleotides showed polymorphic patterns with highly reproducible bands in Artemia urmiana groups and Iranian Artemia groups.The highest percentage of polymorphic loci were in Artemia urmiana (P= 78.89 %) and in comparison with the other parthenogenetic forms, in parthenogenetic Artemia from Saray village (P= 59.8 %). The highest values of Shannon index were estimated in Artemia urmiana (I= 0.41), and in comparison with other parthenogenetic forms, in parthenogenetic Artemia from Saray village (I= 0.3). Analysis of molecular variance indicated that most variation were within populations (A. urmiana with parthenogenetic forms, FST= 0.35) and among populations (Iranian Artemia with foreign species, FST= 0.76). Genetic pattern was not in accordance to geographical distribution, which indicated the wide range of Artemia samples at different regions in Iran. UPGMA showed two distinct clusters in group I including A. urmiana with two parthenogenetic forms and four clusters in group II including Iranian Artemia with some foreign species. It is understood that two parthenogenetic populations (Fesendoz and Saray village) had close genetic relationship together with high value of heterozygosity in parthenogenetic Artemia from Saray village. Iranian Artemia (Qom, Arak, and Golestan) indicated close genetic relationships with A. parthenogenetica (Russia and Turkmenistan) with low values of heterozygosity. A sister group was generated including Shiraz and USA Artemia samples which attests a possible contamination of local water with A. franciscana. FST value revealed high genetic structuring at all studied groups. Besides, it is suggested that gene flow was not strong enough to prevent substantial genetic differentiation. Result could lead to a management programmes to use suitable Artemia strains with high genetic diversity, for culturing and establishment of gene bank of Artemia stocks in Lake Urmia.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study investigates the distribution of three trace elements (Cd, Cu and Zn) in tissues (exoskeleton, abdominal muscle and hepatopancreas) of two shrimp species (Penaeus merguiensis and Penaeus semisulcatus). Special emphasis in this study was placed on evaluating the effects of storage conditions on redistribution of the elements and moreover, the potential roles of metallothionein in tissue redistribution. The specimens were collected from the northern part of Persian Gulf during August 2001 and September 2002. The concentrations of metals in the tissues were measured using an Inductively Coupled Plasma-Atomic Emission Spectrophotometer (ICP-AES) and an ICP-Mass Spectrometer (ICP-MS). Metallothionein levels were determined using Differential Pulse Polarography. Results show a similar hierarchy for Cd and Cu concentrations among different tissues as follows: hepatopancreas〉 exoskeleton〉 muscle; and for Zn, the hepatopancreas again contained the highest levels, though there were not any significant differences in Zn concentrations between muscle and exoskeleton. Significant differences were found between the species in the case of Cd and Zn concentrations. Cd and Zn levels in P. semisulcatus were markedly higher than in P. merguiensis. In general, there were highly significant differences among the storage conditions from accumulation the selected trace elements and metallothionein in both species point of view, but in most cases no significant differences could be observed among the fresh, refrigerated and frozen specimens of P. semisulcatus. In regards to Zn and Cu accumulation in edible (muscle) tissues of P. merguiensis, optimum conditions for frozen storage is -30ºC as ice frozen, and in terms of short term storage, one day refrigeration is recommended. The redistribution of Zn and Cu during the storage period can be attributed to the role of metallothionein in their transport from organ(s) to muscle during storage. The variations in pH values measured in the muscle of P. merguiensis in different storage conditions showed a similar trend with metallothionein, Zn and Cu. In contrast it appears that cadmium is transported from muscle to the other tissues. Exoskeleton is probably one of the recipient tissues of cadmium. The inter-elemental relationships were assessed for each species, separately. A significant positive relationship was observed between the concentrations of Zn and metallothionein in P. semisulcatus, whereas in P. merguiensis, Cd concentrations decreased significantly with increasing metallothionein. Highly significant differences between the sexes in Cu and Zn contents could be detected. Highly significant size-related differences were found in the levels of Cd, Cu and metallothionein in the selected tissues of the shrimp species. Comparison between the mean concentrations of the elements in muscle tissue and existing guidelines indicate that in nearly all cases the concentrations were well below permissible levels for human consumption. The only exception was observed regarding mean values of Cd, which was somewhat greater than some of the recommended levels.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: An artificial Reef is a fabricated, underwater structure, typically built for promoting marine life in areas of generally featureless bottom. Creating artificial reefs began from 2000 in southern provinces in Iran. This study has surveyed about the Bushehr and Khozestan states. The objective is to determine the effectiveness of artificial reefs in attracting and enhancing the production different aquatics through increasing of fish assemblage and fishery. The performance of artificial reefs deployed off the coast of the Persian Gulf in 10 to 15m, was evaluated. The reefs comprised of seven artificial and one control (natural) statistical tests plan in three replicates. Three types of artificial reefs were used in this study. That two were designed reefs and one was not designed or used material. The experimental plan consisted (i) Reef ball (R), designed hemispherical shaped; (ii) Laneh Mahi (L), designed pyramid shaped; (iii) Used material (U); (iv) mixed (RL); (v) mixed (RU); (vi) mixed (LU); (vii) mixed (RLU); and (viii) control site (CS). Trap nets and under-water visual census surveys were adopted for seasonal sampling of fish aggregation. Results of statistical analysis using ANOVA and T-test of the mean Catch per Unit Effort (CPUE) showed significant difference (p〈0.05) in term of computing number of fish for aggregation of fishes. The study has concluded that reef deployments have influenced favorably the fish communities and fish harvests. Therefore, the artificial reefs, especially the mixed RLU, are appropriate tools for future fishing enhancement in the Persian Gulf of Iran.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Use of underground inland water for fisheries aims is improved all over the world. Tilapia is the first candidate for brackish water fish culture and an economic fish based on its biological characteristics. Tilapia was entered to Iran for the first time in November 2008. Bafq area at the center of Iran was licensed by Environment Organization, because of closed underground water resources of the area. Studies showed desired growth and adaptation of Nile tilapia in brackish water earth ponds and fiber glass tanks with high survival and low FCR. Limnological factors were suitable for tilapia culture. All male samples were made using certain doses of methyl testosterone in defined period. Optimum level of protein, fat and energy were determined for the best growth and food performance of cultured tilapia. Environmental program was presented to prevent environmental problems. However, studied shoed tilapia culture had not negative effects in the area. Economical performance of tilapia culture project was desirable. Tilapia was successfully introduced to some private fish farms in June 2011. Tilapia culture may improve in center of Iran after environmental surveys.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: According to achieve sustainable production in shrimp farming industry, it is necessary to provide the relief of producers through insurance. Aquaculture faces various environmental conditions such as unpredictable climate changes, epidemic diseases, harsh water factors, that may affect the production cycle, and impact on producer's life. In this research, the effects of various factors on production of cultured shrimp have been studied. The project conducted through completion of questionaries by random selected of small scale farms, and all large farms which engaged in production in that years. In order to increase the accuracy of responses, special questionnaires provided to fill in by expertise experts.All data explained the share of management and natural factors on production process.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Protecting coastal aquaculture systems as a habitat for aquatic systems is one of the serious concerns of the world. In the present study, spatial and temporal variations of Some physicochemical parameters, nutrient concentration and water quality In some estuaries of East and West of Hormozgan province were investigated from March 2015 to February 2016.Data analysis was performed using multivariate statistical methods (such as: CA,DA and PCA Analysis) and Eutrophication and water quality index using multivariable indices. The average annual variation of various parameters studied in West Azarbaijan province of Hormozgan is as follows:water temperature (18.1-34.6Co ), Salinity (35-45.6 ppt), Dissolved Oxygen (4.6-7.4 mg.l), pH (8.01-8.4), Nitrate (32.5- 403.5µmo.l), Nitrite (3.6-50.7 µmo.l), Total Ammonia (9.8-311.2 µmo.l), phosphate (22-569.1.6 µmo.l), and chlorophyll-a is equal to(0.46-4.04 mg.m3 ), respectively.Also, the mean annual variation of the various parameters studied in the eastern province of Hormozgan has been as follows: water temperature (18.1-34.6Co ), Salinity (35-45.6 ppt), Dissolved Oxygen (4.6-7.4 mg.l), pH (8.01-8.4), Nitrate (32.5-403.5µmo.l), Nitrite (3.6- 50.7 µmo.l), Total Ammonia (9.8-311.2 µmo.l), phosphate (22-569.1.6 µmo.l), and chlorophyll-a is equal to(0.46-4.04 mg.m3 ), respectively. One-Way Analysis of Variance test showed that the significant for some differences physicochemical parameters varied seasonally.Discriminant analysis, water quality conditions in terms of location classified into three groups.The analysis of PCA showed that the amount of nitrate and total ammonia in the tiba and phosphate in Laft and Kgamir Stricks with two sources (domestic and agricultural waste) and natural sources, can be considered as the main factors affecting the intensity of Eutrofication.The range of trophic Trix and TI eutrophication index in the studied areas were 4.3-6.1 and 3.5-4.2 respectively.According to on the indicators of and TI index, trophic status was classified into mesotrophic(in laft and khamir areas), Oligo-Mezotrophic(Yekshoveh area) and mezo- eutrophic respectively. Also Based on Trix index, the Water quality status was from bad condition in tiab area and good condition in laft, khamir and yekshoveh areas respectively. The determination of trophic conditions with a single-variable method based on chlorophyll a and Total inorganic Dissolved materials(DIN) showed a Very good agreement with multivariate trophic indices.The range of changes in some of the parameters studied in this study was more than the reported limit for the conservation of aquatic life in coastal areas.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Striped grey mullet (Mugil cephalus) is a marine fish with high commercial value and widespread distribution. The grey mullet and some other mullets can be found in saltwater, brackish and fershwater habitats at tropical, subtropical and also temporal climates.This species is suitable for aquaculture worldwide due to living in wide ranges of water temperatures and salinities, acceptable growth rate, suitable food conversion ratio, marketability, suitable species for polyculture with shrimps, marine fish and even with freshwater carps. The grey mullet is farming in different countries such as Italy, Northern Africa countries ( Egypt), occupied Palestine, India, Pakistan, Japan, Hong Kong, Taiwan, Vietnam, Indonesia, coastal countries of southern pacific ocean and Hawaii. Ten thousands of fries of this species (average weight and length of 280 mg and 28.3 mm respectively) imported in March 2008 from Egypt. The wild fries colleted from surrounding natural waters of Alexandria. After finishing the quarantine steps, they stocked in four earthern ponds (each with 2500 m2) located at the Gomishan shrimp educational centre at late march in 2008. At the first year of culture the densities were 5000 and 10000 individuals per hectar and each treatment with two replicates. At second years densities changed with 2000 and 2500 ind./hec. and each of traetments divided fishes with average primary weights of 80 and 115 grames. The fries and fish feeding begins in April when the water temperature rise above 18 °C and it cut in fall when the temperature goes under 20°C. They fed twice a day at 8 AM and 2 PM and the amount was 5-7 percents of the fish existent biomass in each ponds. They fed with dry formulated feed made of Mahdaneh Karadj Company (Danso dry feed) both the first and second years of farming. The feed prepared for carps feeding and containded 23±2 % crude protein, 10 ±2 % crude lipid, about 7 % crude fiber, 15 % ash, 40 ±2% carbohydrates and with energy content of 4000±200 kcal/kg. After seven months culture period the average weight and length of fish in treatment 5000 ind./hec. were 113.7 gr and 21.1 cm respectively and in treatment 10000 ind./hec. the average weight and length were 86.6 gr and 19.6 cm respectively. At first year the average survival rate in different treatments was 77 percent. The average FCR in treatments 5000 and 10000 ind./hec. were 3.4 and 3.9 respectively. At second years after six months culture the average weights in pond no. 9 (with primary weight 115 gr and density 2000 ind./hec) , pond no. 10 (with primary weight 80 gr and density 2000 ind./hec) , pond no. 11 (with primary weight 115 gr and density 2500 ind./hec) and pond no. 12 (with primary weight 80 gr and density 2500 ind./hec) calculated as 476.6 ,338.1, 366.5 and 440 gr respectively. The average survival rate and FCR were 93 % and 3.1 respectively. Ponds no. 9 and 11 (fish stocked with primary weight 115 gr in densities both 2000 and 2500 ind./hec) selected as the best economic efficient treatments.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Barcodes are short segments of DNA that can be used to uniquely identify an unknown specimen to species, particularly when diagnostic morphological features are absent. These sequences could offer a new forensic tool in plant and animal conservation especially for endangered species. Ideally, barcodes could be used to positively identify illegally obtained material even in cases where diagnostic features have been purposefully removed or to release confiscated organisms into the proper breeding population. In the present study molecular data were used besides the morphological ones to introduce a specific barcode for six selected species of Shrimp. Based on the previous phylogenetics study, it has been proved that the mitochondrial COI gene in crustacean is a good discriminative marker at both inter- and intra-specific levels. In this regard, six species of shrimp (Fenoro penaeus indicus, Fenoro penaeus merguensis, penaeus semisulcatus, Metapenaeus affinis, Marsupenaeus japonicas, Fenoro penaeus penicillatus were collected from the Persian Gulf and Oman sea. After identification, the total DNA was extracted; COI gene was first amplified and then sequenced for each species. Finally the collected data were analyzed with the specific phylogenetic software. Molecular analysis revealed some degree of interpopulation differences but six good species were recognizable based on COI sequences. Also for population study molecular data of two species Penaeus semisulcatus and Metapenaeus affinis were analysed base on COI RFLP and 16SrRNA sequences respectively. The results would be helpful to protect shrimp species.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The effects of added artificial substrates on juvenile white Indian shrimps (Penaeus indicus) were evaluated. .....

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The shrimp farming success in the future will greatly depend upon increasing supplies of healthy and high quality seed for stocking ponds. The limited and inconsistent availability of wild shrimp postlarvae together with an urgent need of establishing selective breeding programs, increased the interest in captive broods of penaeid. Many investigations have been carried out on several shrimp species world-wide. This study have been conducted on 2 shrimp groups since September (Mehr) to July (Tir) 1999 in Bandar-e-Imam southwest Iran. The wild group (SPB) prepared from Jask area and the pond group (POB) prepared from artificial ponds upon harvesting them as marketable size have been investigated. 2 groups were exposed to natural diets consisting of squid and artemia biomass in same conditions using 12 m3 concrete tanks. Stocking density was 2 piece/m2 (1F:1M). Half part of the pond bottom covered by fine sand to reduce the stress where the other part has been used for feeding. Daily ration given in 3 times by satiation method. Also reproductive performance of wild gravid brooders (WLB) were recorded as a control group. 2 examined groups well grown and around 50% of the SPM and around 30% of POB females successfully ripened a produced eggs and nauplii where almost all males of both groups were matured. Data showed fecundity is highly correlated to the TL and W of the brooders by positive trend. Considering the TL, W, Fecundity and nauplii production the WLB brooders are significantly (P=0.05) higher than 2 other groups, while the SPM broods are significantly (P=0.05) higher in hatchability. Meanwhile the survival rate from nauplii to postlarvae stage was around 30% for SMP brooders. On the other hand the growth parameters (TL, W) of the marketable size of the shrimps produced from SMP brooders were significantly (P=0.05) higher than those produced from WLB group. Since the fecundity is improvable by increasing the brood size thus the data suggest the sup.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Following of aquaculture activities increasing and important, the evaluation of the effect of this activities on marine ecosystem seem to be necessary. ...

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Study of survey health management and diseases in hatcheries and fish farms can help us to knowledge and application control methods such as: prevention, treatment and increase high levels of production in hatchery and farms, finally. This survey carried out from 2005 to 2008 for 4 years in sturgeon hatcheries and farms of Golestan province. Sturgeon fishes include Huso Huso, Ship sturgeon, Acipenser persicus collected and for virology, bacteriology, fungius and hematology examined. Also, physicochemical parameters measured and recorded in different stages of culture. Results of this study showed that all of samples in virology was negative and did not observe any doubetful causes. In bacteriology CFU was variation from 3/9 ×105 to 6/9×10. The most parasites that detected in this survey was Cocolanus espherolanus , Sceria binopsulus semiarmatus and Amphilina fuliacea that separates from Acipenser Percicus, especially. The results about hematology parameters some important hematological indices of ship sturgeon include: The total RBC for female and mail specimens measured as 5.3±1.5 ×105, 4.8±0.5 ×105 per mm 3 respectively. The amount of haematocrit and hemoglobin for female and mail determined: 34.3±2.8, 35±1.4 percent and 10.3±0.9, 8.9±0.8 gr/dl .The MCV: 216.3± 96.2, 736.5± 102.5, MCH: 720.2±309.5, 186±0.7 and MCHC: 30±0.8, 25.5±3.4 percent respectively.The total WBC were (female, male): 21320±1054, 20580±777 per mm 3 and neutrophil: 16.4±2.5, 17±1.4 percent and lymphocyte: 74.4±2.4, 73.5± 0.7 percent and eosinophil: 6±1.4, 6.4±0.5 percent, monocyte: 2.8±0.8, 3.5±0.7 percent. There was not any significant differences (p〉0.05) between mentioned parameters in male and female (students t-test). Also evaluation of hematological parameters in bluga ( Huso huso) include: total RBC were (male , female) 5±0.3 ×105 , 4.9±0.6 ×105 per mm 3 ,respectively and hematocrit: 33.2±6.7 , 35.4±3.4 percent and hemoglobin: 11.2±1.5 , 12.2±1gr/dl and MCV: 669.9±172.2, 723.9±982.4 and MCH: 226.2±42.5, 249.5±35.4 and MCHC: 34.1±2.4, 34.6±3.6 percent respectively. The totals WBC were (male, female): 24800±707.1, 23042±1375.4 per mm 3 and neutrophil: 18.5±0.7, 21.4±1.1 percent and lymphocyte: 73.5±1.4, 68.4±1.1 percent and eosinophil: 5±2.8, 7±1.2 percent and monocyte: 3.5±3.5, 3.2±0.8 percent. According to statistically study the count of lymphocyte had significant difference between male and female fish and this count in male was higher than female. (p≥0.05).

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Protection authorities of plant and animal genetic resources and conservation of biodiversity as national assets by using biotechnology techniques is the most important objectives of genetic data information bank. Establishment of shrimp species genetic data bank, makes possibility of study multiple genes such as economic genes and since knowledge of genetic variation within and among populations and barcoding species of shrimp are very important for species conservation and with so little researches of Asia in the global studies of DNA barcoding and animal taxonomy in Iran, In this study, Iran shrimp genetic diversity and genetic data bank was done. DNA barcode is a short, standard well known sequence of cytochrome oxidase І gene. By using this DNA sequence can be realized that each animal, plant or fungus belongs to which species. Also prepare a bank of genetic data shrimp pathogens and maintenance of these strains are steps to advance future researches in the fields of pathogenesis mechanism of pathogens, diagnosis, treatment, Disease prevention, production diagnosis native kits and detection of emerging and reemerging diseases its origins. So in this plan samples were collected from imported cultured shrimp Litopenaeus vannamei and and 6 Persian Gulf and Oman Sea shrimp species which classified based on traditional systematically as: Penaeus semisulcatus, Fenneropenaeus merguiensis, Metapenaeus affinis, Parapenaeopsis Stylifera and Fenneropenaeus indicus. After examination of DNA barcode sequence, molecular and bioinformatics operations of each sequence in the Consortium for the Barcode of Life (CBOL) and National Center for Biotechnology Information (NCBI), phylogenetic analysis of each sample was determined and similarity of each sample with NCBI and CBOL database was checked and the closest species to each sample were specified. According to the results different samples of L. vannamei, . banded P.semisulcatus, F. merguiensis and F. indicus have more than 97% similarity to the same species of other countries. non banded P.semisulcatus had 80.07% similarity to banded P.semisulcatus, M. affinis samples had 90.3% similarity to Metapenaeus ensis and Parap. Stylifera had 93.44% similarity to Parapenaeopsis coromandelica in the CBOL. This funding confirmed the need for further investigation and possible announcement of new species. By using ribotyping technique, native isolated pathogenic bacteria and fungi were identified and recorded in the gene bank database center. During sampling of shrimp and water of Specific Pathogen Free shrimp center, 40 bacterial strains were isolated, which 8 of them had the most frequency and identification based on 16S rDNA sequencing was performed. Bacteria identified are: Vibrio nigripulchritudo strain IS013(GenBank:KP843725) ،Vibrio brasiliensis strain IS014 (GenBank:KR186076) ،Vibrio rotiferianus strain IS015 (GenBank:KR186077) ،Vibrio azureus strain IS012 (GenBank:KJ018724.1) ،Vibrio owensii strain IS016 (GenBank:KR186078) ،Agarivorans gilvus strain IS017 (GenBank:KR186079) ،Vibrio brasiliensis IS018 (GenBank:KR186080) and Vibrio alginolyticus strain IS019 (GenBank:1817854), which were recorded in The World Bank genes. In this study fungal isolates were not detected.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This investigation conducted for evaluation Silo (Cylindrical) Fish Tanks as an applicable method for aquaculture of Rainbow trout (Oncorhynchus mykiss). Current research was designed in two practical phases as accidental experiments through 3 treatments and repeats of which stocked by three Rainbow trout (375 gr) densities as 75, 100 and 125 fish/m3 in 9 silo fish tanks (2 m3). For better understandings of the silo performance method, concrete circular fish tanks (similar volume) considered as controls and stocked as similar fish densities as silo tanks. The duration of the first phase of experiment was 150 days. The second phase of the experiment was determined for evaluating two proposed silo type (Inlet and outlet in bottom side / Inlet from bottom and outlet in upper side). In this stage, proposed silos (stocked by fish of 294 gr weigh) tested for rearing trout for 30 days. Water supplied from two surface wells after some aeration operations. Stocked fish were fed by pellets made by Fish Feed Chineh Company (Ltd) in Iran. Feeding operation conducted according to the recognized manuals by consideration of water temperature, fish weight and biomass. Meanwhile, biometry operation conducted in 10 days intervals while fish were anaesthetized. In addition, water quality was determined by daily measurements. According to the results of the first phase of experiment, there were significant variations on Growth rate, survival rate, FCR, FCE and SGR through the cultured organisms in silo tanks compared to the circular tanks. Meanwhile, according the results of an economic study on this survey, the fish culture in silo tanks showed significant economical and profitable in compare to controls. In additions, the second phase of experiment showed better aeration conditions in silo tanks when inlet water supply from bottom side and outlet located in upper side. Because of the fine distribution of fish in silo tanks and more usage of depth by this method, fish culture would be an applicable and profitable technique in narrow lands and slopes through the mountains area. The maximum stocking rate recorded more than 100 Kg fish/m3 in silo tanks.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Gillan , Mazandaran and Gorgan provinces have temperate climate, thus they have more potential for aquatic animal culture. There are thousands hectare of lands for Aquaculure and fisheries in adjacent to Caspian Sea in this provinces. these areas(North alborz) have provided a favorable back ground for aquatic animal breeding and stock enhancement of species in the sea. As a result, this investigation executed during two stages (phases). At first stage, the goal of this project included the feasibility study and demonstrating the existing situation of fish stock enhancement activities in North Alborz area and second phase is to survey on distribution, and production activities of stock enhancing hatcheries as well as their constructions,and production time table. Survey was started frome 2006 to 2008 by using qestionaries in 7 hatcheries from tree provinces.results revieled that there are more than 10 species was subjected to restocking .Total releasing was about 300 millions of fry and finger lings in to the Caspian sea.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The effects of aquaculture industry on the environment were evaluated by studying the water quality of Bushehr costal water during culture season 2005 and 2006. The variations of selected parameters such as total phosphorus, ammonia, chlorophyll a, pH, salinity and... Were monitored in effluent canal, influent canal and open sea in Helleh and Mond regions as monthly. Following occurrence of White Spot Disease (W.S.D) in the shrimp ponds of Bushehr province, shrimp culture suspended and entrance of effluent waters completely were blocked since August 2005, despite the absence of aquaculture sewage evaluation of environment and sampling were continued. During releasing of sewage of shrimp ponds to the coastal waters of Bushehr in Jun 2005, the average amount of ammonia in Helleh and Mond were obtained to be 0.161 mg/l and 0.194 mg/l, however the average amount of total phosphorus in Helleh and Mond regions were 0.149 mg/l and 0.043 mg/l, respectively. Although effluent water suspension, amount of ammonia and total phosphorus were increased in both region especially in June 2006. Comparing present data in Jun 2005 and recorded data from culture period 1997-2003 with permitable range of municipal and aquaculture waste show that; aquaculture industry on both regions have not had any obvious negative effect on the coastal waters of Bushehr but it seems if other sources of pollutions in the region is not controlled, then the aquaculture industry can contribute to occurrence of environmental problems.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Study of M. affinis was carried out from June 2001 to December 2002 in two sampling areas of Khouzestan coastal waters. In this period, 18 cruises in Life- Buseif (western coastal area) and 13 cruises in Bahrekan (eastern coastal area) were came by using shrimp trawl net. In Life- Buseif fishing area, the CPUE of total shrimp was the highest (27.8 kg/ h) in September 2001. This parameter estimated 17.4 kg/ h for M.affinis in March. In this area, the highest biomass of total shrimps was In April 2002 (533.3 t). This value was 380.7! for M. Affinis on March. Maximum mean length of males and females of M.affinis were observed m January (13.4 & 11.5 cm respectively). Maximum and minimum sex ratio (f: m) of this species was 2.25: I July and 0.86: I in October 2002. Relative frequency of shrimp in total catch was observed m March (52.5%) in Life- Buseif. This parameter was 27.3% in February in Bahrekan area. In Bahrekan fishing area, total shrimp CPUE was highest (7.5 kg/ h) in March, and max. CPUE for M. affinis was 6.9 kgffl in same month. Maxmum biomass of total shrimp and M. affinis were 1800.4 t and t in March Maximum length of male and female of M.qffinis were 124 and 11.3 cm in March Maximum and minimum sex ratio (f: m) of M. affinis was 3.51 in May and 09: 1 in January, March and August 2002 respectively. According to the results of this study, shrimp catch season was opened in Life- Buseif from 3rd Nov until 29th Dec 2001 and from 6th Nov until 15th Dec 2002 winter shrimp catch season has been closed in Bahrekan since 2001.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In 22 August of 2000 an adequate amount of fresh cow manure was transported form Golshahr Cow Farm and deposited in the experiment site. The transported manure was divided equally between five treatments of siloes, depots without plastic coverage, depots with plastic coverage, barrels and tubes. Deposited manures retained in the treatments till 20 May 2001. During the retention time, sampling from three points of cow farm and other treatments carried out with monthly intervals and samples were analyzed for determination -of Ca, Na, NDF. ADF. crude protein, crude fat, moisture; crude fiber, minerals, dry mater and NFE. Temperature, pH and redox potential were measured in situ and all data were analyzed statistically by SAS general linear models. The results showed that month and treatment exert significant effects (P〈0.01, on the variations of the most measured parameters. Depots with plastic coverage had highest mean temperature and then there were depots without plastic coverage, siloes and barrels, respectively. “The temperature increased significantly in the first month of retention but showed some fluctuations in subsequent 4-5 months and then remained relatively constant till the end of experiment period. Depots without plastic coverage showed highest moisture. Optimum moisture value of 40-50 percent obtained for siloes and depots with plastic coverage. Lowest Ca and highest Na values were measured in fresh manure of the cow farm. In two first month of retention time NDF decreased significantly in most treatment but increased subsequently and reached maximum 'percentages during 5-6th month of retention. In first month of retention ADF showed significant decrease in most of treatments but increased in further months and reached to maximum percentage after 4 month retention. In siloes, depots without plastic coverage, and depots with plastic coverage the percentages of protein and fat reduced significantly in the first month of retention, modified during subsequent 6 months and reach valuable percentages. Depots with plastic coverage showed maximum and most suitable protein and fat percentages of about 9 and 2.75 percent after 6 months retention. The percent of crude fiber decreased to minimum level after 3, 5 and l, retention months in siloes, depots without plastic coverage, and depots with plastic coverage and barrels respectively. Values of surface pH in siloes, depots without plastic coverage vice versa of other parameters were minima of 7.1-7.5 at the beginning of retention time that reached to 7.8-8.4 after 4 retention months. The pH of cow farm mostly was more than other treatments. Amount of redox potential in cow farm, siloes, depots without plastic coverage, depots with plastic coverage and barrels fluctuated between -48 to -109, -84 to -12, -62 to -22, -86 to -30 and -65 to -21 millivolt. The minimum levels were at beginning of period and the maximum levels obtained after 4 months of retention in treatment I and 2, after 3 months in treatments 3 and after 5 months in treatment 4. Therefore with care to extent and terns of parameters changes in variation treatments, depots with plastic coverage can introduce the best retention conditions during 4-6 months for decomposition of organic manure for usage in aquaculture.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Recently, the development of aquaculture has focused on the use of seawater, because of freshwater crisis in the world. Whiteleg shrimp (Litopenaeus Vannamei) is one of the most important species for aquaculture development, because of tolerance and adaptation to different ecological conditions. One of the advantages of species in aquaculture, compatibility status is dense in the rearing period. In this study, the effect of different densities (45/m2 , 50/m2 , 55/m2 and 60/m2 ), of primary stock whiteleg shrimp postlarvae (PL12) on the growth and survival rate were evaluated. Brackish water (10.52±0.43 ppt) was providing from Caspian Sea. The experiments were performed in 12 circular concrete pond with a sandy bed (area: 78m2 ) in four treatments and three replications for each treatment. The experiments performed in a 75-day period. In this study, the water temperature was 27.4±1.79°C. The results showed statistically difference in growth parameters and survival rate among experimental treatments (Duncan test, P〈0.05). Therefore, with high levels of density, has decreased the amount of weight gain and survival rate (SR), specific growth rate (SGR) and average daily growth (ADG). In addition, the treatments were different variations of FCR (P〈0.05) and not depend on the primary stock density of postlarvae. In low density (45/m2 ) were observed the highest growth (SGR=11±0.04 and ADG=0.25±0.01 gr/day/ind.), survival rate and calculate the amount of production per 78m2 equal 43.6±3.3kg (5596±433kg/ha). Therefore, It is possibility that there is commonly increasing primary stocking of density about L. Vannamei postlarvae culture in Iran.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The inactivated of white spot syndrome virus (WSSV) by Gama and Electron radiation and chemical material such as Formalin with antigen property have been done during last year. At the first time the stock of virus prepared and purred it. Six hundred of shrimp Fenneropenaus indicus with average weight 7 to 12 gram collected from shrimp research station in Heleh area and transported to Shrimp Research Institute in Boushehr province. Vibrio parahemolyticus obtained from Veterinary University of Tehran and then lyophilized it. The bacterial V. parahemolyticus inactivated with Gamma, electron and formalin and then injected to shrimp for activated the immune system. The source of viruses was identified through PCR, TEM and histopathology methods from the shrimp infected in 2009 occurrence of boushehr province. The virus injected to crayfish and collected the hemlymph for prepared the stock of WSSV virus. The virus was lyophilized and then exposed to gamma and electron radiation and formalin with optimal dose for inactivated the virus. The shrimp divided to 25 groups and each group consist of six shrimp and the vaccine exposed to shrimp with injected and bathroom methods and the documented the result after 15 days. In vivo virus titration was performed in Penaeus indicus. Inactivation of WSSV was carried out by a gamma cell instrument Nordian, model 220 with dose rate: 4.8 Gy/sec and activity: 20469 Ci. The LD50 of live virus stock was calculated 10 5.4 / ml and the optimum dose of gamma radiation beam to inactivate WSSV was obtained 14-15 kGy. The LD50 of live virus stock was calculated 10 5.4 / ml and the optimum dose of electron beam to inactivate WSSV was obtained 12-13 kGy and and the virus inactivated by formalin 0.5% V/V during 10 minutes.The LD50 of live virus stock was calculated by Karber method 10 3.29 /ml and 10 5.35 /ml, respectively. The V. parahemolyticus was inactivated with 8 KG. The result showed all vaccine during 2 hours have a good effect to shrimp viruses with the102.4 LD50 / 50 l titre. The result showed if the Gamma and electron vaccine increase to feed of shrimp the effect of vaccine is better than when used without feed. The end of experiment our study showed that the Gamma vaccine has the better effect to control WSSV during 2 hours with comparing the others.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: White Spot Disease (WSD) is a important disease due to economic impacts in shrimp industries. Spreading of this disease in shrimp farms can caused a 100% mortality during 3-10 days. Therefore control of this disease is a strategy in shrimp industry. Vaccination is a way to control of WSD. In several years ago during a project several type vaccine of this virus by association of Atomic Energy Organization is produced that among of them the virus inactivated by GAMA radiation had better results in laboratory. To test of this vaccine in field, a research pilot was carried out. Initially 20000 shrimp napliies were obtained from one of commercial hatchery in Bushehr province, then devided to two groups vaccinated and unvaccinated. The vaccinated group also devided . 26and PL12 , group B vaccinated at PL15and PL 5to two groups, group A that vaccinated at postlarva e (PL) Rrsults showed that difference of growth performance and survival rate between vaccinated and unvaccinated without challenge with WSV after 80 days is not significantly (P〈0.05). but survival rate in vaccinated groups after challenge with WSV was significantly (P〈0.05) further the unvaccinated group. Also the results showed survival rate of group B after challenge with WSV is better than group A and this difference was significantly (P〈0.05). this study showed vaccination of shrimp postlarvae with GAMA radiation vaccine can control of shrimp mortality in incidence of WSD in farms.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Without Abstract.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: For the first time white spot disease (WSD) was reported in shrimp farms of khoozestan province, in southwest of IRAN in 2002. Then in 2005 the neighbor province, boushehr, was contaminated. In 2008 WSD outbreak reported in sistan-bloochestan province in southeast of Iran. In 2015 all of southern shrimp farms of country except Hormozgan, the middle southern province, which has remained free of WSD, are being contaminated. White Spot disease suspended shrimp culture in thousands hectares of shrimp farms. Considering that white spot disease has not been observed in Hormozgan province yet, the question is; to what extent environmental and management factors participated in preventing WSD outbreak or cause WSD outbreak. In this study (20102012), the effects of environmental factors and management, stressors that decrease immune system function of shrimp are discussed. In addition, the role of pathogen as the main factor of outbreak is discussed. The goal of this study is to define environmental parameters and management practices associates with outbreak of white spot disease in affected provinces and discover reasons of being Hormozgan province free of this disease. In this study the role of the local environmental factors and management practice stressors in susceptibility to WSD was determine. Both the effects of environmental factors in water of ponds including total ammonia, nitrogen, dissolved oxygen, pH, salinity, transparency, and temperature and management issues related to biosecurity are studied. There were overlaps on physical and chemical parameter values obtained in clear areas with contaminated areas .Results of the data analysis suggest that lack of association with WSD incidence was 7 times greater than WSD incidence despite of disease outbreak in sistan-bloochestan province, so other sources of white spot disease virus incidence was suspected in affected areas. Histopathological examinations and polymerase chain reaction (PCR) tests during project performance did not reveal white spot disease virus evidences in post larvae examined from khoozestan province stocked in farms but disease outbreak was happened in that farms , so we suspected to management practice include feed , pond preparation and carrier of disease . Recorded values of temperature and salinity in some months during inspection in Hormozgan province specified stressful condition that may lead to WSD outbreak, however the disease did not appear. Therefore the hypothesis that the water physical and chemical conditions are reasons to prevent disease outbreak in Hormozgan province is being rejected. The policy of Hormozgan’s fishery authorities, to replaced Fenneropenaeus indicus with specific pathogen free Litopenaeus vannamei, that is more resistant to some of diseases, before incidence of WSD in farms and to before being endemic in the Hormozgan province, made an advantage compare to affected southern provinces that introduced Litopenaeus vannamei after WSD prevalence to their farms. However it does not guarantee to maintain current trend of being Hormozgan province farms free of white spot disease. Therefore establishing the principals of biosecurity are strongly emphasized. Strategies taken by the proficient authorities in preparation of SPF shrimp broodstock can be the most important factor in preventing WSD. Regarding biosecurity principals purchased feed must be free of shrimp head powder. Construction the new shrimp farms should be as far as it could be away from contaminated areas.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The project of "Assessment of different methods of water treatment on decreasing pathogenic bacteria in shrimp hatcheries" achieved by mean of reviewing the effectiveness of various methods on the water treatment to reduce bacterial load of sea water. The project performed in Bandargah region of Bushehr province in order to checking the effect of different methods on the sea water and bacterial population. This project has been done over a year. In this study a total of 138 samples was collected during seven times and was evaluated as well as 7 treatments. In this study, the comparison of the effect of each factors (sand filter, chlorine, ultra violet ray, chlorine combined with ultra violet ray) was analyzed on the sea water by means of disinfection. During this study, other environmental factors such as water temperature, dissolved oxygen, pH and salinity was measured till we can ensure that they don’t have any negative impact on the treatments. Results showed that the density of total bacteria in sea water (Control) was averagely 5187 CFU/ml, this was done while the total density of bacteria in the sea water, reservation pool sample, sand filter water, water that irradiated with ultraviolet rays, chlorinated water and the water that was influenced by both chlorine and ultra violet ray was respectively 5187 2137, 4449 2042, 2782 1335, 1164 692, 143 104, 87 76 CFU/ml. Therefore, reservation pool, sand filter, ultra violet ray, chlorine, chlorine-ultra violet ray each of them was reduce respectively 14, 46, 77, 97, 98 percent of the density of total bacteria or in other words they reduced 0.06, 0.27, 0.65, 1.56, 1.77 log of the total population of bacteria in water. Moreover, the results from the total count of vibrios showed that reservation pool, sand filter, ultra violet ray, chlorine, chlorine-ultra violet ray each of them was reduce respectively 17, 47, 74, 98, 99 percent of the density of total vibrios or in other words they reduced respectively 0.08, 0.27, 0.59, 1.66, and 1.95 log of the total population of vibrios in sea water. Also the bacteria that isolated from each treatment were identified with the use of biochemical methods that totally were includes a wide range of bacteria, the identified vibrios were: Vibrio alginolyticus, V. costicola, V. fischeri, V. fluvialis I, V. fluvialis II, V. harveyi, V. natriegens, V. nigripulchritudo, V. parahaemolyticus, V. plagius I, V. vulnificus, V. anguilarum I, V. campbellii, V. nereis, V. proteolyticus, V. splendidus, and Unknown V. spp. Other identified bacteria belonging to the following genus: Acinetobacter, Aeromonas, Alcaligenes, Bacillus, Borkholderia, Eschricia, Enteroacter, Flavobacterium, Micococcus, Proteus, Pseudomonas, Serratia, Staphaphylococcus, Streptococcus, and Unknown Bacteria spp. By comparing the results, we concluded that the best result was related to treatment that the sea water had been disinfected after the combined effects of chlorine and ultraviolet ray. It was determined that this process in compared with other treatments, had been reduced the total number of bacteria, the total number of Vibrios, the number of sucrose fermentative vibrios, and the number of non-sucrose fermentative vibrios respectively 98, 99, 98, 99 percent. In other words, the number of bacteria was reduced respectively 1.77, 1.94, 1.82, 2.23 log. So in this study, the applied method was introduced as the most effective way of disinfecting sea water. Meanwhile, none of pathogenic bacteria for shrimp including Vibrio harveyi was isolated by using this treatment. Comparing the results showed that the combined effects of chlorine and ultraviolet radiation is the most effective applied method for disinfecting sea water.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Aquaculture is the most important part of Fisheries Organization duties in Iran. During several planning and developing programs, aquaculture productions had a considerable growth. Due to good potential, these productions were higher than the planed one. The North Alborz regions were investigated by four groups of experts. The regions which investigated were including 3 important provinces (e.g. Guillan, Mazandaran and Golestan). The area where investigated was 940 km along with coastal zone of Caspian Sea or 58678 km2 in total. Around 27% of aquaculture production came from this region. However, subdivision of fisheries can play important role in the economics in the region. The fisheries sub-sector has an important role in the economy of the region, and despite the great potential for agriculture and tourist industries in the North Alborz, there is a significant role in the prosperity of the fisheries activities. In the present study shows that Mazandaran and Guillan provinces has more potential in reservoirs and aquaculture production when compared with other provinces. Productions in Mazandaran either in reservoirs were less than Guillan province. Due to lee in reservoirs, Mazandaran had less investment when compared with Guillan province. The figures show that carrying capacity of aquaculture production for 3 provinces (Mazandaran, Guilan and Golestan) was higher than other provinces in the entire country. Due to, large land base, less investment, more interesting of farmers, no needs of complicated technology, farmer would be able to develop reservoirs as consequences to enhance productions. However, developing reservoirs and supporting of it's; can increase the contribution of warm water species in inland water.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In order to obtaine live individuals of cobia ( Rachycentrun canadum ) , coastal waters of Jask, Sirik, Kolahi , Bandar Abbas, Bandar-e Lengeh ,Bandar-e Bostaneh and Abu moosa ,Hormoz and Qeshm Islands have been surveyed from March 2008 to January 2010 in Northern waters of Persian Gulf (Hormozgan province). All in all 60 alive fishes obtained from fishing boats and transferred to Aquaculture Department of Persian Gulf and Oman Sea Ecological Research Institute (PGOSERI), but 50 of them died and only 10 fish survived. They divided in two groups and cultured in separated concrete tanks. Fished were fed with trush fish twice a day at a feeding rate of 4-5 percent of body weight. Culturing period last about 6 months. All the fishes gradualy died through the culturing period. Average of specific growth rate in group 1 and 2 were about 0/49 and 0/77 respectively. The weight gain for group 1 (WG) was %75 for 75 days of calturing time and % 142 for 190 days of culturing time while for group 2 it was %31 for 54 days of culturing time and % 75 for 190 days . Average weight of fishes belong to group 1 reached from 950(g) to 2150 (g) during 6 months, while group 2 reached from 872(g) to 1271/5 (g) in 3 months. Feed conversion rate (FCR) for group 1 and 2 were respectively about 6.9 and 6.8. With regard to cost of trash fish (about 2000 Rials per kilogram trash fish) the per unit feed cost of cobia production were 13613 and 13730 Iranian Rials per kilogram, respectively for group 1 and group 2. Results revealed that obtaining live individuals of cobia is very difficult and also cobia didn t grow well in concrete tanks but if we have better coopreration of local fishermen and also provide some Equipment such as cages in order to rear cobia in the sea , it is possible to achive more succes and obtaining better results .

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The "fisheries and aquaculture industry innovation system" is one of the subsystems of Agricultural innovation system (AIS), also has many characteristics of that system .Since this section contains most of the components and AIS institutions, it can be examined as a part of the national innovation system. Due to the structure of the innovation system in the fishery system, a set of functions and institutions of this system are most important .It is therefore important that the system be analyzed and by reviewing the existing state institutions and functions of this system, the main problems and challenges identified. Understanding of the institutions and functions of fishery innovation system and analyze its problems and provide an improved model for fishery innovation system, is most activity in shaping major policy innovation in fishery sector. This step is very important to conduct innovative activities in the fishery sector. In this study, using a system perspective, the current status of the various factors affecting fishery development was assessed and the main drivers and challenges and solutions to improve the detection and analysis was provided. The results show that during the past eighty years, a number of research centers and educational institutions in the public sector have been developed .but this system, despite having considerable institutional capacity, is still in the middle stages of institutional development. In these years, the policy approach was linear, not system approach, so little attention has been to the development of science and technology intermediary institutions, coordinator organizations, venture capital funds, and the institutions of technology transfer ,particularly in the public sector.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Aquaculture of sturgeon species and their hybrids is being considered as an important substitution for sturgeon catch due to highly decrease of natural populations, artificial propagation and fingerling release in the sea. In this study, big bester, a new hybrid sturgeon (female beluga × male bester) was produced for the first time in IRAN. Sperm of 7350 ± 1682 kg male bester was used to fertilize the eggs of one 54 kg female Huso huso. The fries of big bester and control trestment of beluga were fed by artificial concentrated food (48-50% protein and 15-17% fat) after egg yolk absorbance, a period of feeding on Artemia and Daphnia. Results showed that rearing and feeding of bester broods was efficient to reach the fish to maturation stage and there is an opportunity to collect qualified ova and sperm from F1 generation. Meanwhile sex determination and maturity assessment of gonads were successfully done via laparoscopy method. The comparison of produced big bester fingerlings with control beluga fingerling showed that the weigth of big bester fingerlings has not significant difference with beluga's (p〈 0.05) at the age up to 2 months.At 3 months of age, beluga fingerlings showed higher weight (p〉0.05), but there are faster growth rate in big bester fingerlings from 3 months of age up to 5 months (p〉 0.05) in comparison with belugs fingerlings. Meanwhile no statistically significant difference was found between length of big bester and beluga fingerlings among any age. The results of current study showed the potential of rearing male bester to produce matured broods and collection of their sperm for big bester production.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Aquaculture activity is restricted mainly to rainbow trout in cold waters and carps in warm waters in inland waters of Iran as well as Fars Province. Probably for this reason saline waters of south and southeast Fars have not been used for aquaculture. These rivers are ecosystems with a diverse biota, it is predictable that can be found some there endemic aquatic organisms with a potential for aquaculture. Finding a suitable organism can help increase wise use of this natural resource, and local production and recruitment. However, it is highly needed to investigate various environmental elements prior to any use to obtain an estimation of the environmental consequences, parallel to finding usage potentials. In This study, we investigated Dehram saline river in south of Fars Province. Hydrological and physiographical properties, chemical composition of the river, and aquatic populations were sampled and measured monthly in 3 stations in one year. Salinity, electrical conductivity, and total dissolved solids increased from station 1 to 3, reaching from 6.6 g/L, 14.3 g/L, and 12800 µs/cm to 13.5 g/L, 17.7 g/L, and 16500 µs/cm, respectively. Dissolved oxygen and oxygen saturation were higher in station 3, and in autumn and winter. Nitrite showed lowest values in winter (0.006 mg/L) and highest values in spring (0.013 mg/L). Nitrate showed the lowest concentration in station 3 (0.43 mg/L), but no differences were seen among seasons. Ammonium showed no differences among stations or seasons. Phosphate levels in station 1 and 3 were 0.13 mg/L‌to 0.17 mg/L, with the highest level (0.29 mg/L) seen in the spring. Five species of fishes were identified in the river, which all were widely favored by local people. Some of these have been categorized as edible in previous studies, others as ornamental. On the other hand, intensive growth of an algal species throughout the river demonstrates a potential for algal cultivation. Significant role of algae in production of food for both man and livestock is now well recognized in aquaculture industry.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: At present, the aquaculture industry to provide proper instructions in the field of health management, including production of Specific Pathogen Free shrimp (SPF), require sensitive and reliable methods for the detection and identification of pathogenic microorganisms. Molecular methods which used in the detection of microorganisms have a high discriminatory power in the taxonomy and in relation to libraries in the world. On the other hand, the accurate identification of microorganisms, providing the genetic data bank of shrimp pathogens and maintenance of these strains is the step to promote further research on the mechanisms of pathogenesis of pathogens, diagnosis, treatment, prevention of disease, identify indigenous production kits, diagnosis re emerging and emerging diseases and their origin. Therefore, in this project, by using ribotyping technique, native isolated pathogenic bacteria and fungi were identified and recorded in the gene bank database center. During sampling of shrimp and water of Specific Pathogen Free shrimp center, 40 bacterial strains were isolated, which 8 of them had the most frequency and identification based on 16S rDNA sequencing was performed. Bacteria identified are: Vibrio nigripulchritudo strain IS013(GenBank:KP843725), Vibrio brasiliensis strain IS014 (GenBank:KR186076), Vibrio rotiferianus strain IS015 (GenBank:KR186077), Vibrio azureus strain IS012 (GenBank:KJ018724.1), Vibrio owensii strain IS016 (GenBank:KR186078), Agarivorans gilvus strain IS017 (GenBank:KR186079), Vibrio brasiliensis IS018 (GenBank:KR186080) and Vibrio alginolyticus strain IS019 (GenBank:1817854), which were recorded in The World Bank genes. In this study fungal isolates were not detected.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Hamoun fish, Schizothorax zarudnyi, is an indigenous species of the eastern waters of Iran, which is exclusively found in this region. However recently drought occurrence in the Chahnimeh reservoirs (a semi natural water body) making them vulnerable to extinction. As an appropriate action to address this problem and according to the 3 side contract between Italian Government – UNDP - Islamic republic of Iran Government and the Italian government financial support to reduce poverty in the Province of Sistan-Baluchestan through the reinforcement and dissemination of aquaculture activities the project was developed at the Hamoun Research Unit by the Chahbahar Fisheries Research Center to record the breeding normative of 1 g weight larvae for restocking and other researching purposes. 331 broodstocks of the indigenous species Shizothorax zarudnyi weighing 800-2450 g were collected from the Chahnimeh reservoirs in early autumn, 2006. From 5/3/2007 ( the project is supported and communicated on 2010) , Ovulation was stimulated with three stimulators; pituitary extract (3-6 mg kg-1 body weight), GnRH-A (20-30 mg kg-1 body weight) and anti dopamine (10-15 mg kg-1 body weight) that was given in 2-3 doses to breeders. Of 169 injected breeders , some were injected On mid March of 2007 (12-13 ºC water tempreture) responded to the injection 25% ,while the rest were injected On April of 2008 (14-16 ºC water tempreture) responded to the injection 65%. In the present project of 167 breeders 82 were female and 87 male. Totally 30 female breeders released their eggs in different stages. 20 female breeders released their eggs completely, 3 breeders released half of their eggs and 7 released 1/3 of their eggs. The male breeders just injected in the final dose of hormontrapy and all were ready for releasing sperms however the ovulation in female breeders occurs between 353-428 h ºC and after the final dose of injection. Ripe eggs were stripped from the females and fertilization was done by the dry method. Fertilized eggs were transferred to veis incubators and troughs. Incubation period for eggs differs and larvae hatch out after about 910 days at an average water temperature of 12.5 °C. Maintained at 13-14 °C, complete absorption of yolk sac in Shizothorax zarudnyi larvae occurred after 5-8 days. Larvae were fed with a mixture of powdered milk and egg yolk in this stage followed by decapsulated Artemia cysts and nauplii of Artemia and then on formulated starter diets used for carps . Because the ponds were not ready, larvae were maintained in troughs for about ten days before they were transferred to two 1200 m2 earthen ponds where they reached a body weight of about 1 g. They were then handed over to the Iranian Fisheries department in the region. Larvae were fed with the starter feed SFCO in the earthen ponds. About 350 thousand larvae were stocked in two earthen ponds. Based on the results of present study and other studies we may conclude that artificial breeding in Schizothorax can be successfully achieved at 14-16 ºC in flow through systems using hormone therapy (combination of GnRha and anti dopamine) and larvae could be easily cultured in earthen ponds. However this species exhibits lower growth rates as compared to carps its high expenses could have an important role in economical feasible.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Babolroud River is one of the important rivers for fisheries and environmental aspect that locared in center part of Mazandaran province. The river has a span of 92 km starting from Albourz mountainous and end up at the southern part of Caspian Sea. This study was done for five months (3rd Feb. through 5th July, 2008) for the better understanding of pollution situation such as organophosphorous, organochlorine pesticides and agrochemical fertilizer that drainage from paddy fields and horticultures to the river. A total of three main sites for pesticides and plus five sub-sites for drainage were selected for observation in three different regions of the river (mountainous, plain and estuary). The organochlorine and organophosphorous pesticides measured by GC (ECD and TSD detectors) with US-EPA (508) and AOAC procedure and agrochemical fertilizer parameters were measured by ASTM method. The maximum concentrations of aldrin, lindane, heptachlor epoxyde, DDE and β- BHC (period 1), δ- BHC and endrin (peiod 2), heptachlor and DDT (period 3), α- BHC (period 3 & 4), dieldrin (period 4) were 6.02, 0.85, 0.51, 0.50, 0.22, 0.35, 0.23, 0.50, 0.46, 0.19 and 0.16 µg/l, respectively. The maximum concentrations of four components of organophosphorous such as Diazinon and Chlorpyrifos (period 1), Malathion (period 2), Azinphos methyl (period 3) were 1.36, 0.46, 0.44 and 2.56 µg/l, respectively. The maximum amounts of tree components of parameters of agrochemichals fertilizers indictor such as total nitrogen (period 2, sub-site 5), total phosphorus and orthophosphate (period 4, sub-site 5) and organo-phosphorus (period 3, sub-site 4) were 5990, 1290, 1220 and 336 µg/l, respectively. The maximum concentrations of organochlorine pesticides components in sediments of the river such as lindane (period 2, site 2), δ- BHC (period 1 site 3), α- endosulfan (period 1, site 2), endrin and heptachlor epoxide (period 2, site 2) and DDE (period 2, site 1) were 0.99 0.54, 0.29, 0.19 and 0.19 µg/l, respectively. The maximum concentrations of organochlorine pesticides components in fish tissue of the river such as endosulfan sulfate, lindane, endrin, δ- BHC and DDE were 0.32, 0.29 0.27, 0.25 and 0.21 µg/l, respectively.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Study of shrimp nursery ground in the Northern part of Bushehr province from Farakeh creek to Genaveh The fourth phase of "study of shrimp nursery ground in the Northern part of Bushehr province" was carried from October 2000 to August 2001. Sampling was carried for larval stages by using Bongo net with 500 micrometer mesh size, and for adults with Trawl net of I centimeter mesh size. In 12 stations, 25576 juvenile and adult shrimp and 651.13 larves were collected and identified. Most of these samples belonged to the families of Sergestidae, Penaeidae Caridea. Acetes cfsergestidae family, both in adults and larval stage (57% for adult and 84% for larval stage) were identified. Adults wrere caught only in Ramleh creek in Feb. 2001, and the most presence of Acetes' larvae were also in Ramleh creek in Dec. 2000. 33% of adults were belonged to Metapenaus stebbingii of Penaeidae family. The most presence of this species was in Feb. and Mar. 2001. Although no larvae of this species was found in the field, it seems there is no significance between pH, salinity and area temperature with the presence of these species of shrimp.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Inland water aquaculture activity have been known as an important approach for protein production while reservoir lakes recognized to have a significant potential for this purpose. Zanjan province in Iran with several dam reservoirs is suitable for aquaculture development. This study was conducted on biotic and abiotic factors of two lakes Shovir and Mirzakhanlo in order to determine aquaculture potentially of reservoirs. This two lakes located on different climatic region; Shovir located in region of semi arid area with very cold weather in winter, while Mirzakhanlo is in semi humid area and warm weather characteristic. In this study the plankton, benthos and fishes were assessed and the 15 hydrochemical factors were measured in order to determine the fisheries potential for fish introduction or release. The results showed that the phytoplankton abundance of Mirzakhanlo varied between 1.6 to 45.2 million cell/l. with 32 identified genus. The zooplankton abundance varied between 48 to 632 n/l. and 22 identified genus. The abundance plankton in shovir varied between 1.350 to 34.2 million cell/l. and 240 to 4500 n/l. for phyto and zooplankton respectively. Macrobenthos biomass were 21.4 and 0.34 g/m2 in Shovir and Mirzakhanlo lakes respectively while Chironomidae and Tubificidae families were dominant groups. The lake sediment organic matter were 4.3 and 3.2 % respectively. In ichtiology survey identified 5 and 7 species in Mirzakhanlo and Shovir lakes respectively. The hydro-chemical results indicated no restriction for aquaculture activity and according to trophic model both lakes are recognized as meso-eutrophic and eutrophic. Despite of climatic restrictions, the potential for fish production was estimated about 321 and 151 kg/ha for Shovir and Mirzakhanlo lakes respectively that can be improved by using agricultural and the other native facilities.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Artificial reefs are manmade materials deployed under water in order to improve invironment and increase the exploaitation of fishing area. Usage of artificial technic has developed due to increase of world pupolation and need to supply of protein, aim to restoring of natural spicialy rehabilitation of demersal fishes. It has effecte to increase the production in order sustainable exploitation. Coasts and Islands have destruction due to over harvesting from ecosystems and other activities by humans and natural, These caused many aquatic as demersal fishes has endangered in Persian Gulf and Oman Sea. The artificial reef is one way or method that can improve the environment and restore the aquatic. Iranian fisheries has established an artificial reefs area in west of Kish Island. This area has studied during one year, Data of assembeled fishes and physical sampling were collected in seasonal during spring, summer, autumn and winterd. Ther were tow tritment for sampling as artificial reefs site and a control site. Collected data has analyzed and evaluated by SPSS and Exel. The results showed that there was significant difference between the sites and assembled fishes in artificial reefs were more than control site. Consequently the artificial reefs can be a tool and technic to improve the marine environment and increase the production of fishes, especially the demersal fishes.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This survey was designed in the Helleh region, to understand effects of aquaculture industry on coastal waters of Busher, in 2000. Sediment and water samples were collected from effluent canal, influent canal and sea during the 7 month period (harvest and post harvest season). The variations of selected water quality parameters were controlled monthly. Results show that, in effluent canal, estimated parameters of water was higher than other stations every month, and tend to decreased after being discharged into the sea and got closed to normal level. Comparison of above data with permit able range of municipal and aquaculture waste and comparison of present data with data of normal condition of region, show that, aquaculture industry of Helleh region has not had any obvious negative effect on the coastal waters of Bushehr, in the culture year of 2000.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The culture of Iranian Shrimps began in Bushehr in 1372. Early the green or the pink tiger shrimp (Penaeus semisulcatus) which were found in most habitats of the Persian Gulf and Oman Sea were considered in breeding and reproduction, but due to reproduction difficulties, it was rather replaced by the Monodun imported species, then the production of the white shrimp Metapenaeus affinis and Then P.merguensis in some southern areas of the country such as Hormozgan has began in small scale,but none of these species couldn't meet the economical needs of the consumer society and they were not indelible and dominant enough in the shrimp aquaculture industry of the country until breeding of Indian White Shrimp (Fenneropenaeus indicus) has began in large scale in the provinces of Khuzestan and Bushehr, which are seen as the heart of shrimps reproduction and breeding, and in Hormozgan and Sistan. the incidence of white spot viral syndrome (White Spot Syndrom Virus) led to the import of the western white leg shrimp from the USA (P.vannameii) by the Iranian Fisheries Research Organization (IFRO) in 1383, at the present all southern and northern breeding farms of the country (fundamental measures has been carried out in line of shrimp reproduction in Gorgan province in 1386) has put the breeding of this shrimp species as the single breeding species at top of their agenda. The subject of feeding the breeding shrimps is widespread and regarding to the non-proprietary species there are still some species which contain high protein than the other species in studying the different shrimp species requirements. In Iran due to the scarce factories producing the shrimp's food, the alimentary compound of the breeding shrimps during industrializing years of this type of breeding even after the non-aboriginal western white leg shrimp species being inclusive was stable and through different biological processes including: Naplies which feeds from its yalk and by entry into zoa stage start eating only small-sized phytoplankton. And by entry into the stage between zoa and mysis it feeds from phyto and zooplankton simultaneously and entering the post-larva stage it stars sarcophagi. and after 15 post-larva stage entering the growth and transition stage to earthen ponds feeding by concentrated industrial foods in large and small packages depending on young and adult mouth and due to feeding requirements of every stage the alimentary compound is nearly as follows: Digestible protein, energy, fat, carbohydrates, vitamins and minerals will start and the final product will be released in market Or they will be used in the later years of laying eggs and feeding for brood stock. In feeding section the details of every stage and their food's nutritional needs at each stage and will be fully described.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Accurate scientific and practical plan for achieving the goals of the Islamic Republic of Iran within the framework of Vision development 1404 , is the infrastructure achieving sustainable development of the country . Order to achieve the above mentioned objectives and in order to the comprehensive development plans in the country, Iranian fisheries research organization adjust the fifth developing plan for support of executive related departments in country with mobilization a large number researchers consists of several working groups of ifro affiliated research centers . The fifth developing plan consist of three chapters for report of the forth developing plan and intrudction of research , construction plans and financial support (budjet) for period of 2011-2014 A.C .

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The maine pupose of this research has been prepared and produced of Rotifers .....

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In order to introduce a new sturgeon hybrid to aquaculture industry, the possibility of hybrid production by crossing between Siberian and Persian sturgeons and its comparison with its pure parents using morphometric, molecular, cytogenetic and growth performance were conducted. For this purposes eggs and sperms of two pairs of Persian and Siberian sturgeon were collected and reciprocal crosses with 4 treatments were conducted. In treatment 1, Persian sturgeon as control (male and female of Persian sturgeon), treatment 2 (as type I hybrid) from crosses of male Persian sturgeon with eggs of female Siberian sturgeon in treatment 3 (type II hybrid) using crosses between male Siberian sturgeon and female Persian sturgeon and finally treatment 4 by crossing male and female of Siberian sturgeon were used. For each treatment 200 gr. of eggs were fertilized with one ml. of sperm and morphological, meristic parameters of parents and offspring were recorded. For growth comparison of fingerlings of control and hybrids in 3 different phases were measured. In phase (I), larvae rearing from 46 mg. up to 6600 mg. were conducted for a period of 10 weeks. Samples were collected once every two weeks and biological parameters were recorded. In rearing phase II for a period of 330 days, growth comparison were conducted for fish from 6.5 gr. to 750gr. and biological parameter were recorded once every month, where daily growth rate (gr./day), FCR, Specific growth coefficient (percentage per day), increase of body weight, food efficiency and protein efficiency ration were analyzed. In rearing phase (III), from 600 gr. to 2000 gr. for a period of 43 weeks, similar to phase (II) all biological parameters were analyzed, where 26 morphometric and meristic parameters of pure and hybrid parents and offspring were compared both for individual and ratio status of parameters. For genetic analysis and variation between control and hybrid specimens the microsatellite analysis were conducted. For this purposes, genomic DNA were extracted from fin tissues of brood stocks and fingerlings and 10 pairs of specific primers (Afug 56, Afug 63, Afug 241, Afug 229, OX 27, Afug 686, Afug 195, Afug 12, IS 68 and Afug 160) were used. Then PCR Products were electrophoresed on 6% polyacrylamide gel and then were stained with Nitrate silver and alleles were evaluated based on its length (base pair). Cytogenetic analysis and number of chromosome of control fish and hybrid were compared using white blood cell culture method and chromosome spread of parents as well as offspring were prepared by Gimsa staining and were visualized under light microscope. For sexual gonad development, laparoscopic method with light camera (Model M-CAM 1700) were used to differentiate 34 pieces of control and hybrid fish and gonad status of ovary and testis were analyzed. In present investigation the results indicate that, in phase (I) of larvae culture, a significant differences were observed after 10 weeks of feeding (p≤ 0.05) in hybrid and control groups. Maximum growth rate were observed in Siberian sturgeon larvae, followed by type I hybrid, type II hybrid and Persian sturgeon larvae. In phase (II) , growth rate of Persian sturgeon were lower than Siberian sturgeon. For period of 330 days of rearing periods, the control Siberian sturgeon had 1.4gr. weight increment per day, while Persian sturgeon had only1. gr./day. However the hybrid type (I) had an average daily growth of 1.5gr/day and lower growth performance were observed in type II hybrid with daily growth of 1.5 gr./day. In phase (III), growth rates were varied at different age group. The daily growth rate of Siberian sturgeon was 4.5gr/day, while is control Persian sturgeon was 2.6 gr/day. The Siberian sturgeon within 47 weeks of rearing grown from 655 gr. to 2016 gr., however Persian sturgeon for the same period reached from Siberian sturgeon with male’s Persian sturgeon had higher type II hybrid showed opposite results for the same growing period and reached from 640 gr. to 1680 gr. In conclusion of growth performance can be stated that the hybrid from crosses between female Siberian sturgeon with male Persian sturgeon presented highest growth rate in comparison to control hybrid. This excellent achievement can be considered as a big step toward sturgeon aquaculture for meat and Caviar production. In analysis of morphometric characters 26 parameters were investigated which in 18 parameters showed significant differences (p≤ 0.05) which indicate clear distinguish able of hybrid performance in compare with parents where Siberian sturgeon has longer snout compare to Persian sturgeon and its size are intermediate in hybrids. In relative ratio of characters, out of 9 characters totally 7 characters showed significant differences (p≤ 0.05). In molecular analysis using microsatellite primers totally 400 DNA samples of hybrid as well as the control fish were investigated. Totally 784 allele were detected in which 363 alleles were belong to control fish and 421 alleles in hybrid. In this study, 19 alleles with different sizes were observed in hybrids rang from (120bp) in Aox27 primers (400bp) in AFUG229 primers. In comparing of all primers, the primer AFUG160 and AFUG195 with 2 alleles and primer AFUG686 with 7 alleles showed the minimum and maximum allele numbers.The banding patterns were monomorphic in 66 loci of control fish and 65 loci of hybrids, and in 77 loci of control fish and 73 loci of hybrids were diploid in 9 loci of control fish and 12 loci of hybrid fish were tetraploid, in one locus of control samples and 3 hybrid samples were pentaploid and finally within 10 pairs of primers 7 sets were disomic, 3 primers (Afug241, Afug686, Aox27) were tetraploid and 6 primers of (Afug63, Aox27, Afug688, Afug12, Is68, Afug160) showed excellent allele heritability which indicate the high efficiency of microsatellite techniques. In evaluation of the status of gonad development as well as determination of sexual stages laprascopic method indicated that out of 9 samples of Persian sturgeon (4 females and 5 male) were in stage of 2 & 3 gonad development. Out of 8 Siberian sturgeon (4 males+4 females) stage 2, 3, 4 were detected. In 7 gonad samples of hybrid (Female Persian and Male Siberian) totally 5 male and 2 female were observed at stage 3 and 4 gonad development. In analysis of 10 samples of type II hybrid (female Siberian × male Persian) 3 female, 6 males and 1 deformetic were observed. Cytological studies on 30 metaphase palate from 6 fish samples showed that the number of chromosome in hybrid are as same as parents 2n=250±10 where all control and hybrids similar to its parents showed 240 chromosome. In conclusion, It can stated that, this study successfully introduced a new sturgeon hybrid for sturgeon farming both for meat and caviar production. Considering the common name of sturgeon parents used for production this hybrid, we call this hybrid as (Siper) where Si instant for Siberian sturgeon and Per comes from Persian sturgeon.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: DNA barcode is a short, standard well known sequence of cytochrome oxidase І gene. By using this DNA sequence can be realized that each animal, plant or fungus belongs to which species. in this research, samples were collected from imported cultured shrimp Litopenaeus vannamei and and 6 Persian Gulf and Oman Sea shrimp species which classified based on traditional systematically as: Penaeus semisulcatus, Fenneropenaeus merguiensis, Metapenaeus affinis, Parapenaeopsis Stylifera and Fenneropenaeus indicus. After examination of DNA barcode sequence, molecular and bioinformatics operations of each sequence in the Consortium for the Barcode of Life (CBOL) and National Center for Biotechnology Information (NCBI), phylogenetic analysis of each sample was determined and similarity of each sample with NCBI and CBOL database was checked and the closest species to each sample were specified. According to the results different samples of L. vannamei, . banded P.semisulcatus, F. merguiensis and F. indicus have more than 97% similarity to the same species of other countries. non banded P.semisulcatus had 80.07% similarity to banded P.semisulcatus, M. affinis samples had 90.3% similarity to Metapenaeus ensis and Parap. Stylifera had 93.44% similarity to Parapenaeopsis coromandelica in the CBOL. This funding confirmed the need for further investigation and possible announcement of new species.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study is a part of the national and technology master plan entitled "Attain to technical knowledge of specific pathogen free shrimp production and cut off to dependence on foreign products". The goals of this work were to assess and analysis the risk factors of production of specific pathogen free Litopenaeus vannamei shrimp in its all stages (broodstocking, laravl production, shrimp farming, feeding and water quality), determining the pattern of outcomes monitoring, management and control of outcomes from April 2012 to September 2015 in Bandargah research station and Persian Gulf SPF Shrimp research station and their environment, belonging to Shrimp Research Center, located in Bushehr. Identification and screening of risk factors have been done based on Environment Aspect and Effect Analysis method, (EA)2, using a fine statement questionnaire and quantification of risks. The number of identified risk factors in Bandargah research station was 15 while it was 13 in Persian Gulf SPF Shrimp research station. The more number of risk factors in Bandargah station in respect to its number in Persian Gulf station is might due to lower level of biosecurity, physical structure and aging of Bandargah station and existence of two possible source of contamination, the Bushehr nuclear power plant pollutants and Bandargah fishing harbor, near to the station. On the basis of results, the maximum values of APN in Bandargah station was 42 while it was 30 in Persian Gulf station. These results revealed that the level of risk in Bandargah station is lower than that in Persian Gulf station. The most probable risks in both studied research stations are related to transmission of pathogens in shrimp feeding and water intake pollution.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This survey carried out from 2012 to 2013 to study of suitable site selection for carp and trout fish species for responsible aquaculture possibility by data collection and literature study.During the survey ecological factors including physical and chemical factors,geological factors including topography and type of soil,biological factors including selection of species and control of invader animals,climatic factors,geographical factors and pollutant including heavy metals,agricultural pesticides Surfactants and crude oils were recognized.Also in comparing with standard levels suitable rate of each important factor in aquaculture were studied.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: After severe mortality due to white spot disease incidence in cultured shrimps of Helleh grows out farms, disease spread to Delvar,Mond, Boeyrat and Bandar Rig sites in Bushehr province during 2005. Many crustaceans keep this virus in the body, act as a carrier, and can spread of the infection. In order to investigate status of contamination of wild shrimp and crab's population to white spot virus in Bushehr province waters (north of Persian Gulf), samples were collected and tested by Nested PCR for detection of WSV from estuaries and input and output channels water around the Helleh, Shif and Mond sites during 2007 and 2008. Total tested shrimps were 401 peaces, includes green tiger shrimp (Penaeus semisulcatus) 220 pieces, white shrimp (Metapenaeus affinis) 181 pieces, also total tested crabs were 292 pieces includes Ocypode rotundata 68 pieces and Portunus pelagicus 224 pieces. All the samples showed negative results of WSV contamination. However, it can never be sure that the population is free from WSV unless we collect a random sample of 300 pieces from each species and test them individually for WSV. Therefore, we will be confident up to 95% that the wild sources in that area have no prevalence of 1% or upper WSV contamination.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Scientific and practical planning for achieving the lofty goals of the Islamic Republic of Iran in the framework of development plan of 1404 is the requirement to achieve sustainable development. Establishing roadmap of industries and products was considered by the scientific and technology office of the deputy president, in order to achieve the abovementioned objectives and in line with the national scientific comprehensive map. Therefore, present study was conducted as the roadmap of national aquatic-food processing development using available approved documents e.g. Iran perspective of 1404, fifth developing plan of I.R. of Iran, developing plan of Iranian fisheries organization in 1400 and the available approved statistical data. Current perspective of the aquatic-food processing is presented at the first chapter of the study. The second chapter reviewed the problems and obstacles. Third chapter tabulated the published studies and researches in fish handling and processing so far. Finally fourth chapter recommends executive and research projects considering quantitative and qualitative objectives of Iran 1400 plan as well as fifth developing plan of Shilat-Iran (Iranian fisheries organization). Authors hope the study to be useful in the national planning of I. R. of Iran in the future.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In this study ichthyoplankton, phytoplankton, zooplankton, benthics and hydrochemistry parameters were studied. 9 out of 11 sampling stations were in the Gorgan Bay area and 2 of them were in the mouth of the Bay. Ichthyoplankton survey were carried out in 6 stations and the other surveys such as phytoplankton, etc were done in 11 stations in 2009. Gorgan Bay map and position of sampling sites are given at the end of material and methods section. Biomass and frequency of phytoplankton at different stations, months and seasons were calculated that are presented in Tables 1 to 3. Hydro-chemical parameters such as water temperature, air temperature, visibility, DO, BOD5, pH, Alkalinity (bicarbonate, carbonate and total) , Hardness (calcium, magnesium), CL,EC, Salinity, N (nitrate, nitrite, NH3), Phosphate were measured and results are shown Table 11 . Biomass (mgr/m3) and numbers (ind./m3) of 47 genus of different phylum of phytoplanktons such as Cyanophyta, Chlorophyta, Pyrophyta, Chrysophyta and Euglenaphyta as well as different phylum of zooplanktons consist of Protozoa, Arthropoda, Rotifera and Cyliophora were estimated. Frequency of phytoplanktons (ind/m3) was 131.2, 1.6, 65.3, 18.9 and 23.8 millions respectively. Furthermore, their biomass was 308.4, 1.9, 1358.9, 295.1 and 124.7 mg/m3, respectively. The maximum and minimum numbers were observed in Leptolingbaya belonged to Cyanophyta and Diatoms of Chrysophyta 84.3 and 0.025 million ( ind/ m3) respectively. The maximum and minimum biomass was observed in Gyrosigma (Chrysophyta) and Tetradron (Chloropyta), 12317.3 and 0.014 mg/m3 respectively. The average biomass of zooplankton was measured 531.74, 30.77, 225.07, and 96.26 mg/m3 respectively and the total was attained 883.83 mg/m3 Table 5 . The maximum and minimum biomass of all zooplanktons was estimated in November and July, 3446.23 and 6 mg/m3 respectively. Furthermore, the maximum and minimum numbers of zooplanktons were found in September and July which were 48.4 and 0.002 millions respectively and the average number was 8.4 ind/m3 Table 4 . Also numbers and biomass (gr/m2) of 11 families of benthos which inhabit Gorgan Bay such as Annelida, Mollusca and Arthropoda were investigated in separated stations and distinctive months. The maximum and minimum biomass of benthos was observed in January (42.91 gr/m2) and March (0.6428 gr/m2). The biomass was considerable in April, February, August and December (Tables: 4, 5 and 6).

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was conducted to codify the guideline and indexes of broodstock management in salmonid fish and cryopreservation, selective breeding and sex reversal in hatchery to attain responsible aquaculture development. Cryopreservation includes of sperm and egg in term of economic is important in aquaculture. In this report mentioned the type and name of material. All female and sex reversal fish are produced by steroid hormone which mentioned in this report. Selective breeding include individual selection and family selection which could be used for genetic improvement of fish.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: An Environmental Impact Assessment was undertaken to identify existing values and characteristics of physical, biological and socioeconomic resources within the area proposed for the Aquaculture development in BibiShirvan Ab-bandan (small storage dam). The proponent (Fisheries Department of Golestan province) intends to redevelop fish culture in a constructed water body which is used mainly by local farmers for storing water to meet crops water needs especially at dry periods of the year. The area being examined as part of this environmental impacts assessment is located approximately 40 km west of Gonbad city,Golestan province in northeast of Iran. The site located at 54° 47'- 54° 52 ' (East longitudes) and '37 ° 10'- 37° 11' (North latitudes). The Bibi Shirvan Ab-bandan sealed with an area of about 580 acres, is a collection of three distinct basins that are connected with each other at several points. At present, there are some problems for water supply and maintaining full reservoir levels which cause the Bibi-Shirvan reservoir have always been empty partly and farmers could not been utilizing all its capacity. At the operation phase, the supply water will be provided by pumping through the left coast of Gorgan river via an irrigation concrete canal which is constructed by the Organization of Jihad-e-Agriculture and also, two small streams namely Ghareh-tekan and Bayram-shally. For better management of fish culture it is proposed a plan, which increase the volume of the Bibi- Shirvan reservoir by heightens the circumference embankment and construction of the interior retaining wall that will divide the reservoir into smaller pieces of ponds. It also proposed the installation of other pumping stations on abovementioned streams to supply water for combined requirements of cultivated crops and fish culture. The total surface area and storage capacity of Bibi Shirvan reservoir will be about 500 hectares and 8.1 million cubic meters respectively, after the construction of embankments. Environmental impact assessment for the project in terms of design features is mandatory and subject to article 2, paragraph (e) environmental impact assessment regulations. There are some problems for developing optimized fish culture practices and operations in the area. The unknown ownership of reservoir, absence of official right of water allocation, limiting capacity of reservoir reduced surface area for fish culture , lack of hydraulic system structures for water supply control, and drainage are among the problems that restrict fish culture practices and will not allow to attain optimum production. Therefore, the proposed plan including improved methods and rehabilitation of embankments was placed on the agenda of the Golestan Province, Department of Fisheries. For optimization studies and achievements to update information using Geographic Information System (GIS), the maps of the study area were provided. Thus, in first step, the digital map with a scale of 1:500 to 1:2000 AUTO CAD format that shows the project location, have been constructed. General location map of the region, residential areas and villages, roads and other maps needed in studies using these layers were produced. Next, the outputs for Cartography maps were prepared using ARC GIS software. The spatial layers were performed by using the GIS soft-wares and advised which suggested by different expert teams (groups of soil and water pollution studies, social environment, economic and cultural, biological environment, climate and hydrology). The output of digital spatial data, spatial analysis and cartography as various maps, tables or diagrams and graphical analysis were used in the reports. Since implementing this Project could be created various impacts in different environments (physical, biological, socioeconomic and cultural) The EIA study was undertaken .For facilitate decision making process on project implementation, the modified matrix method has been used. In conclusion, the results of EIA matrices shows the action of Project with environmental mitigation measures (+410) is superior to no action Project (-79). It is therefore considered that the Bibi-Shirvan Project will have a minimal impact on the environmental values of the area. The Project will provide favorable overall positive outcomes for indigenous peoples and fish farmers in the region. Also, problems such as excessive migration of rural people, lack of employment and consequently low level of income and welfare of the people will be improved. Therefore, the Project with consideration of environmental mitigation measures is recommended .

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Effects of S. cerevisiae on immune parameters of the L. vannamei after 14 days of S .cerevisiae feeding were evaluated in this study. For this purpose a total of 300 pieces of shrimp with an average weight of 30 to 35 grams were selected from a pool shrimp Abadan CHOEBDEH. After making sure the health, absence of necrosis on the surface of the body, cuts antenna, shrimp were transferred to the center of BANDAR IMAM Research Station. Adaptation was carried out for 3-5 days. After the adaptation, shrimps were screened for virus (WSSV, TSV, MBV, HPV, YHV, BP, IHHNV and IMNV) and vibrio bacteria.After screening shrimps divided to two groups with three replication (including 50 pieces of shrimp in triplicate). The experimental diet has the commercial shrimp composition ,but 2 g of S. cerevisiae substituted 2 g of fish meal. Shrimp of first group (T1) for 14 days with food containing nutritional yeast and shrimp in second group (T2) were fed with normal diet without yeast. After 14 days Immune Factors and survival rates in both groups were evaluated. The results showed that the relative survival rate between the two groups showed no significant difference. But Immune Factors (THC, TPP, PO, POD and SOD) in the treatment fed yeast (T1) compared to control treatment (T2) showed a significant increase. In conclusion these results suggest that the increased survival rate and resistance of shrimp after S. cerevisiae consumption occurs through immune modifications, such as increases in THC, TPP, SOD, SOP and PO activity.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Present study was conducted in shrimp farm located in east of Chabahar in Sistan and Balouchestan province from August 2001 until May 2003. Fourteen stations at three locations (i.e. supply channel, drainage channel and Gwatr Gulf) were selected to determine physical and chemical factors such as; temperature, salinity, dissolved oxygen, pH, nitrate, nitrite, ammonia, phosphate, silicate, total suspended solid and turbidity. Sampling was conducted twice a month during the shrimp culturing period and monthly at other times. Water samples were collected every two weeks to determine Chemical and Physical factors, Chlorophyll a, Phytoplankton and Zooplankton. Sediment samples were collected seasonally to examine Macrobenthos, Grain size and Total Organic Matter. The results revealed different bioenvironmental area in the three locations (i.e. supply channel, drainage channel and Gwatr gulf) over the study period. One-way analyze of variance of chemical and physical factors indicated significant difference between different times as well as different stations (p〈0.05). The negative relationship was observed between the culturing production area and the amount of average dissolved oxygen, pH and total suspend solid. An increase in the culturing production area in 2002 as compared to that of 2001 causes a decrease in the amount of these factors. However, it dose indicates positive relationship with nitrate, nitrite, ammonia, transparency and chlorophyll. Forty nine phytoplankton genus were identified, which mainly belong to diatoma, dinoflagellate and green blue algae. Diatom was dominated among these groups. Diatom and green-blue algae and diatom comprised %79, %69 and %64 of phytoplankton flora respectively in supply channel, Gwater Gulf and drainage channel. Copepod and thintinnida were the major groups of zooplankton. High abundance of bivalve, Polychaetes and amphipoda were found in supply channel, Gwatr Gulf and drainage channel respectively. Analysis of grain size showed the existence of sandy sediment in Gulf of Gwatr and sandy-loom in supply channel. Drainage channel sediment found to be sandy-loom, sandy-silty-loom and sand loom. Among three locations, the highest TOM was found in drainage channel. In last location, production area in 2002 increased as compared to that of 2001, which showed negative relationship with salinity, nitrate, nitrit, ammonia and chlorophyll a. Drainage of the Gwatr s shrimp farm did not cause any thermal contamination in the location. Shrimp aquaculture activities caused an increase in the average of salinity, range of pH in the Gwatr Gulf.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The study on economic aspects of tilapia culture in Iran is a subproject of a comprehensive project on introduction of tilapia to central regions of the country. Two types of red and black of the exotic tilapia were imported from Indonesia in 2009 for research and were transferred to the research center of Bafgh. Since tilapia has capabilities in aquaculture such as resistance to diseases, fast growth, tolerance to high intensity, growth in freshwater, brackish water and in saline water, also ability to grow in pond, cage and aquaculture tanks, so it is likely that it’s production be of economic advantages. Therefore the present study looked at economic aspects of raising tilapia. The factors examined were feed expenditures, wages, fertilizer spending, costs of fish fingerlings, and pond preparation in two treatments of black and red tilapia in 0.45 hectare earth ponds, 3m2 aquaculture tanks with two replicates. Results of data analyses showed that benefit of each pond for black tilapia would be about 5968763 Rials. For red tilapia this amounted to 5939696 Rials. On total the benefit for black tilapia pond culture was 11940444 Rials while this for red tilapia was 11879392 Rials, so we can conclude that pond culture of tilapia is beneficial. Culture cost of black tilapia in tanks with 57 Kg outcome, was 2540600 Rials. Accordingly the cost of each kilogram of fish is 44572 Rials, so with 20 percent benefit, the price for each kilogram is 53485 Rials. Also for producing 37 kilograms red tilapia in tank some 2531500 Rials was expended. Each kilogram red tilapia cost 68419 Rls to produce which is much more than other cultured fish and if we expect 20 percent benefit the price of each kilogram of red tilapia would be 82103 Rls. So it is concluded that tank fish culture of tilapia would not be beneficial and economically speaking there would be no point to raise tilapia this way.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This project was performed to evaluate the qualitative and quantitative operation of hatcheries and shrimp culture Sites in Hormozgan province during years 2004-2006. All the active hatcheries including one hatchery located in Bandar-e-Jask, Three hatcheries in Bandar-e-Kohestak, one hatchery in Bandar-e-kolahi and two hatcheries in Qeshm island also three shrimp culture sites including Tiab-e-Shomali, Tiab-e-Jonubi and sayeh khosh were surveyed. The Information about scientific and technical persons, employments, productions, important problems and also situation of costs and net were recorded in special forms. Results were analyzed by descriptive statistics. Results revealed that about 50% of the hatcheries located in Hormozgan province are closed through the different problems such as, failure of customer, failure of a distinct plan for post larvae producing, high coast of producing parameters and so on. Results also revealed that only 7.1% of farms located in sayeh khosh area, about 40.2 % of farms located in Tiab-e-Shomali and 62 % of farms located in Tiab-e-Jonubi areas are active. The two later areas have established about ten years ago. The most problems of most farm in Hormozgan province are the failure of sufficient knowledge of farms managers, high cost and low quality of shrimp foods manufactured by huge factories and low cost of cultured shrimps. Final, it can be said that problems of hatcheries are depended to shrimp farms problems. If problems of farms be did, problems of hatcheries will be did.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Pearl aquaculture production is one of the most valuable industry in the world. Black lip pearl oyster Pinctada margaritifera is one of the most important commercial species in the world that it is used for breeding and production of pearl. To determine the basic requirements for black lip oyster growth and development of gonad under controlled conditions, Pearl oysters with a total length of 80 mm shells that were in stage 2 maturation of the island Hendorabi collected by diving operations and were transferred to Bandar Lengeh Research Station Persian Gulf mollusks. Pearl oysters with length of 95 mm shells, was passed were all male. 25 percent of them with a total length of 100-110 mm and all with a total length top of 110 mm were female. Then thay were fed for 45 days in appropriate conditions of temperature and photoperiod with March until late spring. Ggreatest physical growth related to Diet with micro-algae Isochrysis sp (T.ISO), Cheatoceros calcitrans, Cheatoceros mullerii, Pavlova lutheri and Tetraselmis suecica, which with the other treatments were significantly different (p〈0.05). Most gonadal growth of the group 4 (Isochrysis sp (T.ISO), Pavlova lutheri, Cheatoceros calcitrans, Cheatoceros mullerii ) was 70% in the treated females were spawning. Minimum total length of black lip oyster in habitat of 80 mm, which were collected from a depth of 2 meters. There is no relationship between total length Oysters and depth of 4 to 10 meters depth and minimum total length was 115 mm. The most density of Black lip oyster were observed in the depths of 4 to 7 meters in the northwestern part of the island Hendorabi. There were stronger correlation between biometric data, length-weight relative to length- length.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was carried out in Bushehr province waters (49ْ 40E and 30ْ 00N to 52ْ 00E and 27ْ 40N) in July and August 2010-2011. The objectives of this study were to determine the opening and closure periods of shrimp and to estimate of the shrimp biomass in the study area. Based on the total length, the opening season of shrimp fishery in 2010 and 2011 were proposed on 12 and 22rd of July, respectively. The closed season was determined based on the remaining of 20% of shrimp stock and proposed on 12 and 29th of August, respectively. The primary estimation of the biomass in these years was 840 and 455 metric tones, respectively. While the total catch were estimated 847 and 859 tones in these years. During this study 9 species of the penaeid and solenoserid species were identified. The percentage of the green tiger prawn to the other species in the study years were 73% and 92%, respectively.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was done in 2008 on agricultural sewage for aquaculture in six stations. Two stations were in AhvazKhorramshahr road near sugar cane plantation sewage in west Karoon, Two stations were in Ahvaz-Abadan road in sugar cane plantation sewage in east Karoon and Azadegan sewage and two stations were in agricultural sewage in the north of Ahvaz. Monthly Samplings of planktons, physical, chemical parameters were taken during Aprile to March 2008. In addition seasonal sampling of benthic fauna and fish were carried out. Heavy metal and toxic samples were done only one time during the study period. Measurement of some parameters such as temperature and pH were done during sampling procedure and other parameters were measured in laboratory based on standard methods. Heavy metal concentrations were measured with polarography and voltammetry techniques and toxic substances were extracted and concentrated to determine the concentration of them in water using GC device. For identifying phytoplanktons, 1 liter water from middle depth was sampled in each station and 4% formalin was added. Zooplankton were sampled monthly using 55µ plankton net. Fish samplings were done using cast net regarding regional condition, and sampling from benthic invertebrate of river were done using Petersen grab with 15.5cm*15.5cm. Results of water quality index (WQI) from Koushk Talaieh (station1), Artificial lake station (station 2) , Farabi(station 3), and Azadegan aquaculture sewage station (station4) showed that water quality is in group four. WQS index was used for Maleh(station 5) and Pumping station ( 6 station) stations due to high levels of salinity. The WQS results showed that water quality of these stations were moderate to low. Heavy metals in water, the ordination is as follows: Ni 〉 Zn 〉 Pb 〉 Cu 〉 Co 〉 Cd 〉 Hg Mercury levels in all cases were less than the standard and all the elements have been somewhat less than chronic toxicity level. The values of all studied metals are lower than the WHO limit. The results indicated that chlorinated pesticides in all samples are at low level and they are at acceptable levels for aquaculture. In this study, 34 genus of phytoplankton from Bacilariophyceae (diatoms), Cyanophyceae, Chlorophyceae and Dinophyceae were present with 11, 8, 14 and one genus respectively and the percentage of frequencies were respectively 37.64, 34.26, 23.24 and 4.87%. In general phytoplanktons of wastewater were in modest condition for using in aquaculture. In this study, about 1,000 samples of zooplankton in 6 stations were identified and counted during the year. The high percentage of zooplankton were belonged to three groups of protozoa (mainly ciliates), Rotifer and copepods were. The Rotifers with more than 90 percent frequency had the maximum prevalence. The dominant rotifer species was Brachionus calciferous. Although in this study the dominant group of zooplanktons was rotifers but the frequency is not high. Therefore we cannot call this circumstance as eutrophic condition, however the growing trend of high food phenomenon has announced. High benthic diversity was observed in Artificial lake wastewater station and Azadegan aquaculture sewage station. The most frequent taxa were in Azadegan aquaculture sewage station during autumn and winter which belonged to resistant Chironemidae larvae. In Koushk Talaieh, Farabi and Maleh stations, Chironemidae was dominanted and in Pumping station Coleoptera was dominant group. Hilsenhoff index showed that most of waste water stations were in poor to very poor condition, indicating that they were heavily contaminated. The data analysis of macrobenthose data in wastewaters based on Satropi and biological value (Z) showed that potential of fish production in Azadegan and artificial lake was high during the year. These two water sources have suitable situation for potential production and biological value, therefore with special plans for culturing low demand fish would be possible. In this study, 7 fish species belongs to 7 genera from 4 families were collected. Freshwater and marine water species was observed in the catch composition. According to the results of the four studied stations, Artificial lake station and Farabi had the highest number of catch which mostly belonged to Acanttopetrus Latus and Liza Abuo. In general, based on biological parameters, such as zooplankton frequency, all stations had similar conditions but in terms of phytoplankton, Azadegan aquaculture sewage station, Pumping stations and Farabi wastewater have better condition for aquaculture. The FBI index showed that the artificial lake station and pumping station are more suitable for aquaculture activity. Low demand fish is recommended for Azadegan aquaculture sewage station. Artificial lake station had lower primary production but it is more suitable for aquaculture because of its lower FBI index value. Most of the fish presence was in the artificial lake station and Farabi wastewater which belonged to two predominant species of Acanttopetrus Latus and Liza Abuo. In general, based on biological and non-biological parameters, artificial lake station in the west Karoon and Azadegan aquaculture sewage station have better water quality than the other stations for aquaculture activities. This requires management action and special schemes.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Barbus sharpeyi is a local fish of Khouzestan that is planned to be a target for aquaculture in recent programs of fisheries organization. Considering importance of this species in its dispersal region, the main goal of this project was evaluation of possibility for triploidy induction and its potential in response to the heat shocks, efficiency of viability and growth and finally reporting the best condition for triploidy induction in Benny. Induction of thermal shocks was executed in for cold and heat shocks (2 and 4°C for cold and 34, 36 and 38°C for heat). Time of induction and its duration varied between 2 and 5 minutes after the fertilization for 3 and 5 minutes. Each treatment was repeated for 3 times. The ploidy level was determined based on size of nucleus diameters in erythrocytes. Analysis of data was done by SPSS (ver. 16) using T-test and ANOVA method. Results showed that the maximum number of triploid individuals was obtained in treatment of 38°C, 2 min after the fertilization by duration of 3 minutes but as the condition was not suitable for the viability of the eggs, losses of the larvae was high in this group. The best efficiency of triploidization in B. sharpeyi belongs to the 34°C, 2 to 5 minutes after the fertilization for duration of 5 minutes. Nuclear dimensions showed an increase in triploids and confirmed that this characteristic can be used as a reliable factor to distinguish polyploidy. Results of this study showed that B. sharpeyi has the ability for polyploidy inductions specially heat shocks. Evaluation of growth in matured fishes, use of proper tagging systems to distinguish the treatments and designing a plan for bioconserving and genetic improvement of this species is recommended.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This project investigates bacteria effects as probiotics on growth and survival rate and resistance of shrimp during propagation stages. Bacterial flora were extracted from seawater , water of cultured farms , shrimp cultured and sludge from private propagation farms different bacteria such as Vibrio alginolyticus(serotype1-4) Vibrio splendidus I , Vibrio fluvialis ,Vibrio anguillarum ,Vibrio costicul, Vibrio vulnificus,Vibrio nereis,Vibrio camplbelli,Vibrio natriegens,Vibrio proteolyticus, Vibrio plegius ,Vibrio fischeri,Aeromonas hydrophila, Aeromonas schuberti,Aeromonas salmonisida, Aeromonas veronii , Pseudomonas pseudoalcaligenes, Pseudomonas chloraphis, Alcaligenes faecalis, Bacillus megaterium, Bacillus pumilus, Bacillus circulans, Bacillus cereus, Bacillus subtilis, Bacillus licheniformis, Lactobacillus case, Lactobacillus bulgaricus, Lactobacillus delbrukii, Lactobacillus fermentum, Lactobacillus leichmanii, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus lactis, Pediococcus acidilactici were isolated , identified and lyophilized.These bacteria in different experiments were used on shrimp life cycle stages and live food such as Chaetoceros , Skeletonema and Tetraselmis algae. Some serotypes of one bacterial species showed various reactions in media and experiment probiotics. Vibrio alginolyticus(serotype1) with 107 cells/ml can increase Tetraselmis sp production during 6 days to 71% compared to the control experiment without bacteria. Also Vibrio alginolyticus(serotype4) with 105 cells/ml can increase Tetraselmis spp production during 6 days to 389% more than control. Vibrio splendidus could increase 23% shrimp fork length in postlarvae 3 compared control. Vibrio alginolyticus+ Vibrio fischeri can increase survival , fork lenght and body weight of green tiger shrimp postlarvae significantly(P〈.05).

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In order to produce broodstock from cultued shrimps , two groups of 1247 and 3781 ....

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The research conducted for finding of aquaculture potential and appropriate areas of Yamchi dam downstream river and estimate of its production in 2010-2012. Yamchi dam is located at 25 kilometers of southwest of the Ardabil city. This research done by using of available information and data obtained from the monthly and seasonally sampling. After evaluating of Yamchi river potential and its marginal lands, suitable locations for aquaculture determinated and theirs production was calculated at each site. Totally, 3 areas selected for trout aquaculture and varius method suggested including race way, octagonal pond and semi-recircultaion. In the present condition of Yamchi river the amount of trout production is predicted at least 65 ton by race way to maximum 1750 ton by semirecircultaion method. Whiles, if 2 cubic meters of water from Yamchi dam outlet is provided to aquaculture, it will be predicted at least 580 ton by race way to maximum 4000 ton by semi-recircultaion method

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: White spot syndrome virus (WSSV) is the causative agent responsible for huge-shrimp viral epidemics in shrimp farms throughout the world.Our study was aimed to determine the effect of WSSV Gamma-vaccinated Litopenaeus vannamei in occurrence of apoptosis. One thousand and twenty PL15 were randomly distributed among 2 treatments and two control groups. Gama-Vaccinated shrimp and none-Gamma-vaccinated ones were our treated and untreated groups. Our result showed there is a significant difference (p〈.05) between vaccinatedexposed group (82.33±2.51) and none-vaccinated exposed group (26.00±10.00) was about 56%. It is concluded that apoptosis can be a helpful process as immune function in shrimp especially against WSSV.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The shrimp farming success in the future will greatly denpend upon increasing supplies of healthy and high quality seed for stocking ponds. The limited and inconsistent availability of wild Shrimp postlarvae together with an urgent need of establishing selective breeding programs, increased the interest in captive broods of penaeid. Many investigations have been carried -out A on several shrimp species world-wide. This study have been conducted on 2 shrimp groups since September (Mehr) to July (Tir) 1999 in Bandar-e-lmam southwest Iran. The wild group (SPB) prepared from Jask area and the pond group (POB) prepared from artificial ponds upon harvesting them as marketable. size have been investigated. 2 groups were exposed to natural diets consisting of squid and artemia biomass in same conditions using 12 m concrete tanks. Stocking density was 2 piece/m2 (IF: IM). Half part of the pond bottom covered by fine sand to reduce the stress where the other part has been used for feeding. Daily ration given in 3 times by satiation method. Also reproductive performance of wild gravid brooders (WLB) were recorded as a control group 2 examined groups well grown and around 50% of the SPM and around 30% of POB females successfully ripened an produced eggs an nauplii where almost all males of both groups were matured. Data showed fecundity is highly correlated to the TL and W of the brooders by positive trend. Considering the T WI Fecundity and nauplii production the WLB brooders are significantly. (P=0.05) higher than 2 other groups, while the SPM broods are significantly (P=0.05) higher in hatchability. Meanwhile the survival rate from nauplii to postlarvae stage was around 30% for SNIP brooders. On the other hand the growth parameters (TL W) of the marketable size of the shrimps produced for SMP brooders were significantly (P=0.05) higher than those produced from WLB group. Since the fecundity is improvable by increasing the brood size thus the data suggest the superiority of the SPM brooders.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Streptococcosis is an acute infectious disease that causes mortality in marine and freshwater aquacultures. One of the most important hosts is susceptible to the disease of rainbow trout and during the past decade its industrial production has been growing in Iran. According to FAO, Iran is among the 10 countries producing fish in the world. The most important bacteria causing Strptococcosis includes Strptococcus, Vagococcus and Enterococcus genera. But in all cases, the clinical sings are the same. More than a decade has passed since the first report of this disease in Iran and unfortunately, this disease has become the most important problems of trout production in Iran. Therefor, 72 farms were selected in 8 provinces including Mazandaran, Gilan, Lorestan, CharmahalBakhtiyari, Fars, Kermanshah, Tehran and Kokiloye-Boyer Ahmad and a total 520 moribund and apparently healthy rainbow trout (weight 50 – 200gr) were collected during summer 2008 to 2009. Fish kidney, spleen and liver samples were culture aseptically and finally 206 isolates were identified as gram positive cocci. Using conventional biochemical test, S.uberis, S.agalactiae, S.dysgalactiae, S.faecium and S.inia. Additionally, 172 from 206 isolates were confirmed as S.uberis ، S.agalactiae ، S.dysgalactiae ، S.faecium and S.inia using a PCR assay. Based on the results, the most prevalent is belong to S.uberis (isolated from 5 of 7 province), S.dysgalactiae (isolated from 4 of 7 province), S.agalactiae (isolated from 2 of 7 province) and S.faecium and S.inia (from 1 of 7 province) respectively. It is necessary to mention, S.inia was identified just to Fars and any gram positive cocci was isolated from Tehran.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: During 2006 to 2007 Litopenaeus vannamei was dominant cultured species in Bousher and Khouzestan .....

Description: Published

Description: Aquaculture is one of the most dynamic activities of agriculture sector, and it seems the most economic part. Additional needs of population to white meat and healthy protein, increase of employment, achievement of foreign exchange sources, and environmental friend ship development are basis for sustainable aquaculture worldwide. Growing rate of aquaculture within last two decades, shows highest level among agricultural products, and have been two digits for many years. Trend of aquaculture development in Iran shows same path, and devoted high share ness of the water bodies. Warm water fish which have highest share in aquaculture production, are mainly produced in freshwater. Warm water fish production in the year 1382 was 61000 tones, and increased to 167000 tons in 1392. These figures for warm water fish produced in 4025 farms with an area of 22323 ha in 1382, and developed to 14615 farms with area of 48697 ha in the year 1392. Production of this fish shows highest amount in the country, and have high economic and export values. Cold water fish, which Rainbow trout- Onchorhynchos mykiss as the main species shows additional growth in various parts of the country, so as the production in the year 1382 was 23000 tones and increased to 143000 in 1392 (one decade). The number of farms was 562 units (89.8 has) in the year 1382, and increased to 1923 units (230 has) in the year 1392, which indicates another sign of fast growing. To achieve sustainable aquaculture in aquaculture, preparation the manuals aquaculture on Best Management Practice, feed and feeding management, Fertilizations and Enrichment, Stocking methods, Pond preparations, Aerations, Site Selection and Farm Establishment, Marketing are considered , preparation of manuals for uxorious parts of production chain is essential. Site selection, Process of establishment of farm, use of mechanization for production, providing optimum oxygen levels, selection of brood stocks , are main parts of responsible aquaculture

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was carried out in August – september 2009 in Bushehr province waters. Two different shrimp bottom trawls were used in which were woven by Poly Amid and Poly Ethylene with mesh sizes( stretched ) of 50 mm and sack with 35 mm. Also 2 different vessels of two dhows and one steel ship each with 26 and 30 hauls were used and taken in to comparison from point of obtained results. Total catch of PA and PE trawels were 4592 and 5535 kg of which the ratio of shrimp catch and bycatch for PA trawl was 32.4 and 67.6 % respectively; and for PE trawl was 34.2 and 65.8%. Total catch of PA and PE trawls of steel trawler ( ship ) were estimated 3158.0 and 4784.7 kg, respectively, of which the shrimp/bycatch ratio was 6.6 : 93.4%. There was a higher amount of bycatch for PE comparing to PA trawl (p〈 0.05). on the other hand the decrease of shrimp and bycatch for PA comparing to PE dhow trawlers were 21.3 and 15.0% and for ship were 34.6 and 34.0% respectively. The weight ratio is 1:15 i.e . in PE ship trawl , in proportion to on kg shrimp, 15 kg bycatch was harvested. it is proposed to standardize the mesh size and also to do obligtory the instal of squared mesh size panel .

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The blue swimming crab Portunus pekagicus linneus, 1758, is Known as a by catch of trawlers, Gillnetting,... in the coastal waters of the Persian Gulf and Oman Sea, Blue crabs are not considered as target catch in the Iranian fisheries. The best method for catching these crabs is in this survey. Two kind of posts including Japanese shrimp pots and collapsible pots were tried. Also gill net was used. Result showed that Japanese shrimp pot and collapsible pots were determined as the best gear, Samples catched by these pots have larger mean lengths and weight, they are mainly undamaged.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The present survey was conducted for evaluation of environmental impacts of shrimp farm effluents in Tiab district of Hormozgan province. During the growing season of 2003(Jul-Dec) water and sediment samples have taken with monthly intervals from inflow and outflow of the farms and related coastal waters adjacent to Tiab estuary. The samples were carried to the laboratory for physical, chemical and biological analyses. The results showed that the farms exert significant influence on humidity of surrounding air. Furthermore most of the measured parameters such as pH, salinity, BOD5, COD, ammonia, nitrite, nitrate, phosphate, TP, total phytoplankton and zooplankton numbers in water column and the percentages of the TN of sediments of the outflow waters were adequately higher than inflow water, but reverse results were measured for oxygen content of the water and total macrofauna numbers. There were no any significant differences between inflow and outflow channels in the case of temperature and TN of the water. In the statistical analyses, station effect only showed significant influences on variation of zooplankton numbers, TN, COD and salinity of the water and the percentage of sediment total organic compounds. The results also showed that during the growing season in the surveyed channels the most values of water and air temperature, air moisture, salinity and COD decreased, but in contrast the magnitude values of pH, BOD5, nitrite, nitrate, phosphate, TP and total phytoplankton and zooplankton numbers increased during the same time. The month effect significantly influenced the preceded noticed parameters, but had no significant effect on variation of other parameters such as: TN and total macrofauna of the water column and percentages of total organic compounds and nitrogen of the sediment. The results showed that there were no significant differences between the surveyed channels and coastal waters in the case of measured values of water and air temperature, pH and oxygen content. Higher total phytoplankton numbers were measured in the coastal waters, but most of other surveyed parameters were high in the channels. In the coastal waters the variations of most measured parameters were managed by winds and the related waves and turbulences, so water exchange with Tiab estuary had no significant effect in this respect.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Streptococcosis is an infectious bacterial disease that causes huge economic losses in cold water aquaculture industry. Disease outbreak was experienced in some of provinces farms in recent years. Fars Province, has produced 7,000 tons of cold-water fish. According to Streptococcosis report in 1381 from the province and Proceedings have been performed during 10 years against disease and also economic losses impact of disease on rainbow trout production, risk assessment of Streptococcosis conducted on the plan. In this study, of 586 sick fish (have symptoms) studied 230 fish (39.24%) Streptococcus and (26.62%) gram negative bacteria were isolated. Of 754 healthy grower fish and fry (with no clinical signs) 10 fish or fry (1.32%)infected with streptococcus and 60 fish or fry(7.95%) infected with gram negative bacteria (Yersinia ruckeri , Pseudomonas , entrobacteriaceae).According to biochemical tests and molecular examinations , isolated Streptococcus identified as Lactococcus garvieae , Streptococcus iniae and Streptococcus sp. Furthermore some physical and chemical parameters measured and aerobic bacteria of selected farm water counted. These factors effects on disease incidence and changes were evaluated by applying logistic regression.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In this survey the Physical and Chemical factors, heavy metals,agricultural pesticides and microbial contaminations were studied in mohammadiye channel extending Eslamshahr, Robat karim and Shahriar cities of Tehran Province during the 2011.Specimens were obtained from 5 different stations .The measured factors were temperature,DO, Cl,HCO3,COD,BOD,CO2,Mg,……. The analysis of Physical and Chemical factors of Mohammadiye channel water showed that except water temperature,all factors in comparison to the standards were at the standard level,So they are suitable for aquaculture. The result of measurements of heavy metals including lead, copper,Iron,Cadmium, Zinc and mercury in spring, summer and fall in comparison to existing standards showed that unfortunately except copper the amount of other heavy metals are higher than standard levels in the most of stations , Lead amounts at stations 2,3,4 and 5 . zinc at stations 1 and 5 , Iron at stations 1,3 and 5 , cadmium and mercury in all stations were higher than the standards for fish culture. The measured amounts of organophosphate and organochlorine pesticides and result of microbial contaminations culture were less than standard levels. Due to mean of water temperature that is high from middle of Spring to the end of summer , It is suitable for culture of warmwater fishes and because of low water temperature in the station No1 It is recommended for culture of coldwater fishes by using of 50-70gr of young fishes .

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: 30000 juvenile Indian majour carps (Labeo Rohita,Cirhinus merigala, Catla catla) imported (2007 and 2008) and transferred to the earthen ponds in Astaneh Fisheries Research Station (Gilan ) and Sheiban Resaerch center (Khozestan) , In order to assess the viability of rearing fry up to 1 gram fingerlings under the climatic condition of north and south part of Iran . Growth parameters from the larval stage to 1 g were studied also .The fry with 300 mg weight, were released in to 3ponds.specimens were separated to tree experimental categories. fishes were fed with artificial food and also with the natural products of the ponds which were enriched with organic fertilizers, after being equally stocked in the ponds .During the experiment, oxygen level ,PH, turbidity, and temperature were measured . Monthly fry samples were taken, for weight and length biometrical analysis and also determination of the weight gain percentage, average daily growth rate, specific growth rate. Results have indicated adaptations and considerable growth in this species, with an increase from the initial weight of 300 mg to an average of 13.5±1.6 mg in 50 days period until the end of November for fingerlings.Catla was gain 1 g. during 15 day while Roho and Merigal were gain 1 g. during 14 and 10 days respectively. Results revealed that the Indian majour carps compatibility with the condition in Gilan province was succesfull and the combination of species and optimization of their compatibility specialy in other provinces like Sistan and Baluchestan were remind for futhre studies.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Regarding to breeding the Litopenaeus vannamei, a total of 126 pairs of broodstocks were imported from Hawaii to Iran in 2004 and 2005 , and then transferred to the Bandargah Research Station in Bushehr. The female broodstocks were ablated, and were feeded 3 times per day with cuttlefish ,small size shrimp and Nereis worm with a ratio of 30% body weight .The water exchange were done 3 times per day. During the years 2004 and 2005 a total 1700000 naupli were produced of which 772000 specimens of pl13 and pl7 were harvested and then transferred to Helleh Site for carrying out the next culture project. The average naupli and postlarvae were 170000 and 92000 in proportion to each broodstock. Also the mean survival rate was estimated 54%.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Marine polychaete worms living in different beds from coastal areas, so different cultures can be different effects on their development. The evaluation of this research initially, marine polychaete worms were collected in low tidal sea areas from Gulf Persian coast in Bushehr. During several stages of sampling do more than 2000 pieces of worm were taken and then transferred to Bandarga Shrimp Research Station (Iran Shrimp Research Center). So based on a observation the desired species identification (Perinereis nuntia). Then 400 pieces worm selected and transferred to tanks maintain immature worm (fiberglass tanks with dimensions of 2 × 2 × 0.5 m with 15 cm sand on floor). The results showed that even the worms (P. nuntia) in vitro reached sexual maturity able to spawn in captivity conditions and were absolute fecundity of 16010 ± 17630 eggs. After incubation eggs, larval culture into the plastic tank with 70 cm diameter, larval (1.5 to 2 mm) were introduced with density 2000 (pieces/m2). In this research used to three different beds ( tidal , filled with water all of time and wet bed ) with three different food types (food shrimp, seaweed and vegetable waste), so there were 9 experimental treatments. All experimental treatments harvested and estimate production in each of them after 270 days. The seaweed tidal treatment with amount of 21.17 g/m2 had best results and the all of wet treatments with 0 g/m2 was the lowest production. Compare of beds showed that the tidal treatments have significant difference with other beds in growth factors (e.g. mean weight, survival percentage and total production) (P〈0.05). And for effect of food kind on growth factors, the result of difference foods in tidal treatment were analyzed. According this mater any treatments have no significant difference together in growth factors (e.g. mean weight, survival percentage and total production) (P〉0.05).

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The lack of sustainable management programs has resulted in the drastic decline of sturgeons stocks in the Caspian Sea in the past two decades. Legal catch quotas for all Caspian littoral states has dropped from 28500 tons in 1985 to 460 tons in 2007 while caviar production in the Caspian Sea during the same period decreased from 3000 tons to 70 tons. Caviar production in the I.R. of Iran dropped from 305 tons in 1985 to about 11 tons in 2007. The "Strategic and applied research planning for sturgeon management and conservation" was developed with the collaboration of academicians from universities, researchers, experts, fishery authorities and representatives of the executive government to maintain sustainable development and rational management of sturgeon stocks in the Caspian Sea. The preparation and development of this planning was carried out through 121 sessions (2100 person hours) A comprehensive report (375 pg) was prepared which was divided into five volumes; Volume I comprises an introduction, objectives, strategies and planning, present status of production and caviar harvest, catch and export quotas, revenue generated and release of sturgeon fingerlings, Volume II comprises analysis of the problem by constructing a problem tree with 8 broad categories to analyze 344 problems and an objective tree which is the hierarchic flowchart of objectives with 9 broad categories with 241 items, Volume III comprises a review and analysis of previous and ongoing research (414 projects) on different aspects of sturgeon during the past 40 years, Volume IV comprises prioritizing research objectives outlined by the objective tree and finally Volume V which prioritizes primary objectives for 14 executive and 10 research programs. On the basis of the evaluation of the present status and for the sustainable development and rational management of sturgeon stocks a strategic and applied research planning program was proposed and developed within the framework of three primary objectives; 1) Management and sustainable use of Caspian Sea resources (5 programs), 2) Rehabilitation and restoration of stocks (5 programs), 3) Development of aquaculture (4 programs). The applied research program for each proposed executive program was outlined in 10 programs, 42 comprehensive plans and 222 projects. The primary objective on management and sustainable use of Caspian Sea resources includes 5 programs, 14 comprehensive plans and 63 projects, while the primary objective on rehabilitation and restoration of stocks includes 4 programs, 16 comprehensive plans and 87 projects. The primary objective to address sturgeon aquaculture includes 1 program, 12 comprehensive plans and 72 projects. The impacts and outcome of each of these programs was determined and presented. It is evident that if the present situation persists, the catch figures for adult sturgeon specimens and caviar production in Iran will reach zero in 2021. But if concerted efforts are taken and the proposed strategic and applied research planning program is executed (commencing from 2009) we can put a halt to these declining trends. By producing sturgeon fingerlings to restore population abundances, by conserving and protecting them in the Caspian Sea and by conducting applied research we can produce 206.4 tons of caviar by the year 2033. Apart from harvesting caviar from the Caspian Sea we can also annually produce 3000 tons sturgeon meat and 60 tons farmed caviar starting from the year 2023. The total budget proposed for implementation of this program for a period of 14 years (2008-2022) is USD 2483 million. If this budget is allocated in time and the proposed strategic program is properly and totally implemented, we can not only save many sturgeon populations from extinction but also ensure job opportunities for 6000 fisherman, provide 1480 new job opportunities and annually produce 266 tons of caviar which will generate USD 4957 million.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The plan entitled "designing and establishment of ISO/IEC17025 in laboratories of three research centers affiliated to Iran Fisheries Research Organization" was carried out in the frame of three projects from March 2008 for about three years. Among the laboratories of The Persian Gulf ecological research center, the Caspian Sea ecological research center and the national shrimp research center 7, 5 and 3 laboratories were selected for accreditation, respectively. The quality manuals of the test laboratories were written following the requirements of ISO/IEC 17025. During the establishment of the system standardization of the test methods, calibration of the equipment, test methods validation and update and improvement of physical conditions of the laboratories were performed. The technical and quality management competence of the laboratories affiliated to the Persian Gulf ecological research center were evaluated by the auditors from DGA (the German Accreditation System for Testing Laboratories), which is signatory of ILAC (International Laboratory Accreditation Co-operation). But in the case of the two other centers, the laboratories were accredited by Iran Accreditation System (IAS). The laboratories officially accredited, after the successful completion of the accreditation process (including implementation of corrective actions for the detected non-conformities). Production of reliable results of the test methods from national and international scientific organizations' point of view as well as facilitation in development of bilateral and multilateral relations with them can be considered as the advantages of the system establishment. Moreover, exchange of the relevant scientific information and experiences among the researchers will be facilitated

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: There are many factors affecting aquaculture insurance in Iran. In spite of existing formulation for cold water fish, warm water fish and shrimp culture in Iran, there was several aspects and new revisions were remind. In this study we focused on risk assessment as well as insurance procedure to find the better management practices on running insurance in the country. 360 farm(for carp in tree provinces Guilan, Mazandaran, Khozestan),150 (for trout in tree provinces Fars, Mazandaran, Chamahal ) and all 340 farm(for shrimp in four provinces Sistan ,Hormozgan, Bushehr, Khozestan) were selected for risk analysis program. Data were collected throw questionnaire forms. The project was carried out in Two disciplines 1) risk analyzing for manageable and unmanageable accidents and their deductible rates 2) new formulation for insurance management.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In this report rules and focal points about code of conduct for cold water farm, warm water establishment, mechanization management and oxygen generators are collected and discussed. One of the most important point difference between aquaculture engineering with mechanization. Mechanization is improvement of management level from ancient to better by some special tools but aquculture engineering can do by any vessel in every management.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This project is trying to investigate water contamination after use in ponds. For this aquaculture status, physical and chemical water properties, microbial pollutants and heavy metals levels in the some of trout ponds effluent in East Azerbaijan province were evaluated in 2007 to 2009. The information of groundwater and climate conditions in ten years, Drilling and wells piezometric Information, in local wells are reviewed.According to data obtained from analysis of water samples in the output pools range studied concentrations(term mg/l) some parameters are as follows:(BOD: 1.9-3.2),(COD: 3.4-34.1),(TSS: 26.6-42.7),(N-NO2:0.003-.136),(P-Po4:0.0170.067),(pH:7.75-8.28).Electrical conductivity(500 ±2 to 1129±144 term µm/cm) in the effluent ponds . Based on the results of we can say that between input and effluent ponds in the review of all factors as chlorine, sulfate, sodium, potassium, total nitrogen, soluble phosphorus, total phosphorus, calcium, total hardness, magnesium, alkalinity and silica significant changes not be observed. Review of water analysis data to some factors(such as:water temperature,EC,pH,NO2,CO2,HCO3,CO3,DO,COD,BOD) And compare this data with the authorities standard confirmed that the All factors mentioned in effluent is allowed now. In terms of microbial contamination and heavy metals ( Zn,Cu,Ca,Pb,Fe,Cr ) is no pollution. Appropriate strategies for removing suspended materials must to use.We can use Watershed Management solutions to inject output water to the underground table. Should be noted status change (eg change in flow rate, an unusual increase in temperature, fish density increasing, reducing food quality, lack of appropriate management actions and ...) may reduce the effluent quality and the ability of injection water underground to lose . In this case, measures must be for wastewater treatment and improve the quality ponder.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Due to lack of access to comprehensive and up-to-date information regarding equipment, personnel, laboratory services tariffs and chemicals in the laboratories of Iran Fisheries Research Institute and felt the necessity of having this information for optimal management of the laboratories, it was decided with regard to all information needs in this area and using previous experiences in this regard as well as the experiences of other institutions and related organizations at the national (and even international) level, the system would be designed. The main purpose of implementing this plan was to establish and implement a software system based on web technology to create a comprehensive database of the Fisheries Research Institute's laboratories, so that in addition to organizing information, using periodic reports on various related areas a more appropriate monitoring and management system could be applied. Fortunately, after the design and implementation stages, the system in early January 2011 was fully applicable. The main parts of this system include portal; database; advanced data entry, search and reporting capabilities; direct tests order system and discussion forums.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The aquaculture activity in provinces of north of Iran, in spite of fast progressive and possess a series of achievement and success, there was some failure, difficulties, limitation and restriction. To planning for further expansion in this faild, this study is managed. The culture of rainbow trout is practice in individual farms, small farms, water resources, earthen ponds, recycling ponds etc, which each method have its potential , failure and limitation that are consider in the present work. At the end, based on the situation of production in past years, and considering each of factors mentioned above, the success, failure, difficulties, limitation and restriction points of aquaculture of rainbow trout in technical poin of view are investigated and some command for improvement for each production method are presented. The development of aquaculture in recent years is based on extention of farms and increasing dencity of fish in fish pond. In contrast of many production activities, the propagation and culture of fish is inconstant work. This variability makes a potential for better income, occupation and job, increasing in production etc. This study make a better planning using this activity.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Rainbow trout is only species of cold water fishes which is rearing and propagation in Iranian fishery farms. Along of develop aquaculture industries increase request of best produces such as trout eyed eggs and larva. For this reason fishery farmers must be to provide that the way of import of other countries, such as: Denmark, Norway and France. As for this importance subject and unknown basic information about preference ratio for doing it, we needed to study about that to answer the suppliant questions. In this study carried out 3 test groups (3 treatments with 3 repetitions) and compared specific growth rate (SGR), condition factor (CF) and survival rate (SVR) between in this groups. Statistical analysis were significantly difference of increase rate of length and weight in French group with other groups (p〈0.05). Compared of main factors (SGR, CF, SVR) in French test group were significantly difference with other groups (p〈0.05). Survival rate percentage in Iranian treatments (SVR=%67) were significantly with the others (p〈0.05). This ratio respectively in Danish and French treatment was %54 and %48.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The purpose of this study was to evaluate Total petroleum hydrocarbons (TPHs) in surface sediments of Khamir, Laft, and Yekshabeh and Tiab esturies. For this purpose, sampling of surface sediments was carried out at 16 stations (four stations at each estury.In each sample, four points were taken from the beginning, the middle, the end and the junction point to the sea.The physicochemical properties of sediment such as grain size and total organic matter (TOM) were measured using standard methods. The concentration of total petroleum hydrocarbons was determined using Ultraviolet Fluorescence (UVF). Petroleum hydrocarbons showed limited variations from 53.11 to 2052.5 ng/g dry weight in the summer season and from 128.5 to.4392.5 ng/g in winter. The average detected concentrations for total hydrocarbons were lower than the environmental hazard (4000 ng/g). Most of their concentrations were observed at Yekshabeh estury in winter, possibly due to the pollution caused by the activity of ports and fishing vessels. Also, the assessment of environmental hazard caused by pollution of hydrocarbons for aquatic organisms by using SQSs and contamination coefficient (Cf) showed that the studied sediments except station 1 at Yekhshabeh estury in winter season (Cf = 0.91) has no adverse biological effects on aquatic organisms. Finally, the results of statistical analysis of the data showed that the physicochemical parameters of sediments (grain size and total organic matter (TOM)) and the total petroleum hydrocarbons were not correlated, which could indicate the existence of different sources for each studied parameters.

Description: Iranian Fisheries Science Research Institute

Description: Published