Publication Date:
2006-04-08
Description:
Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Petrasek, Jan -- Mravec, Jozef -- Bouchard, Rodolphe -- Blakeslee, Joshua J -- Abas, Melinda -- Seifertova, Daniela -- Wisniewska, Justyna -- Tadele, Zerihun -- Kubes, Martin -- Covanova, Milada -- Dhonukshe, Pankaj -- Skupa, Petr -- Benkova, Eva -- Perry, Lucie -- Krecek, Pavel -- Lee, Ok Ran -- Fink, Gerald R -- Geisler, Markus -- Murphy, Angus S -- Luschnig, Christian -- Zazimalova, Eva -- Friml, Jiri -- New York, N.Y. -- Science. 2006 May 12;312(5775):914-8. Epub 2006 Apr 6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of Experimental Botany, the Academy of Sciences of the Czech Republic, 165 02 Prague 6, Czech Republic.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16601150" target="_blank"〉PubMed〈/a〉
Keywords:
ATP-Binding Cassette Transporters/genetics/metabolism
;
Arabidopsis/cytology/growth & development/*metabolism/physiology
;
Arabidopsis Proteins/genetics/*metabolism
;
Biological Transport
;
Cell Membrane/metabolism
;
Cells, Cultured
;
Gravitropism
;
HeLa Cells
;
Humans
;
Indoleacetic Acids/*metabolism
;
Kinetics
;
Membrane Transport Proteins/genetics/*metabolism
;
Mutation
;
Naphthaleneacetic Acids/metabolism
;
Phthalimides/pharmacology
;
Plant Roots/physiology
;
Saccharomyces cerevisiae/genetics
;
Tobacco
;
Transfection
;
Transformation, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink