Publication Date:
1993-01-08
Description:
Oncogenes discovered in retroviruses such as Rous sarcoma virus were generated by transduction of cellular proto-oncogenes into the viral genome. Several different kinds of junctions between the viral and proto-oncogene sequences have been found in different viruses. A system of retrovirus vectors and a protocol that mimicked this transduction during a single cycle of retrovirus replication was developed. The transduction involved the formation of a chimeric viral-cellular RNA, strand switching of the reverse transcription growing point from an infectious retrovirus to the chimeric RNA, and often a subsequent deletion during the rest of viral DNA synthesis. A short region of sequence identity was frequently used for the strand switching. The rate of this process was about 0.1 to 1 percent of the rate of homologous retroviral recombination.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zhang, J -- Temin, H M -- CA-07175/CA/NCI NIH HHS/ -- CA-22443/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1993 Jan 8;259(5092):234-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉McArdle Laboratory for Cancer Research, University of Wisconsin-Madison 53706.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8421784" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Base Sequence
;
*Cinnamates
;
*DNA Replication
;
DNA, Viral/chemistry/genetics
;
Drug Resistance/genetics
;
Genes, Viral
;
Genetic Vectors
;
Hygromycin B/analogs & derivatives
;
Kinetics
;
Mice
;
Molecular Sequence Data
;
Moloney murine leukemia virus/genetics
;
Neomycin
;
Plasmids
;
*Proto-Oncogenes
;
RNA, Viral/analysis/genetics
;
*Recombination, Genetic
;
Repetitive Sequences, Nucleic Acid
;
Retroviridae/*genetics/physiology
;
Transfection
;
*Virus Replication
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
