Publication Date:
1993-03-19
Description:
Cytotoxic T lymphocytes (CTLs) specific for conserved viral antigens can respond to different strains of virus, in contrast to antibodies, which are generally strain-specific. The generation of such CTLs in vivo usually requires endogenous expression of the antigen, as occurs in the case of virus infection. To generate a viral antigen for presentation to the immune system without the limitations of direct peptide delivery or viral vectors, plasmid DNA encoding influenza A nucleoprotein was injected into the quadriceps of BALB/c mice. This resulted in the generation of nucleoprotein-specific CTLs and protection from a subsequent challenge with a heterologous strain of influenza A virus, as measured by decreased viral lung titers, inhibition of mass loss, and increased survival.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ulmer, J B -- Donnelly, J J -- Parker, S E -- Rhodes, G H -- Felgner, P L -- Dwarki, V J -- Gromkowski, S H -- Deck, R R -- DeWitt, C M -- Friedman, A -- New York, N.Y. -- Science. 1993 Mar 19;259(5102):1745-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cancer Research, Merck Research Laboratories, West Point, PA 19486.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8456302" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Base Sequence
;
DNA, Viral/*genetics/therapeutic use
;
Gene Expression
;
Genetic Vectors
;
Histocompatibility Antigens Class I/immunology
;
Immunization
;
Influenza A virus/*genetics/immunology/isolation & purification
;
Lung/microbiology
;
Mice
;
Mice, Inbred BALB C
;
Molecular Sequence Data
;
Muscles/metabolism
;
Nucleoproteins/*genetics/*immunology
;
Orthomyxoviridae Infections/microbiology/*prevention & control
;
Plasmids
;
*RNA-Binding Proteins
;
T-Lymphocytes, Cytotoxic/immunology
;
Transfection
;
Viral Core Proteins/*genetics/*immunology
;
Viral Vaccines/*genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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