Publication Date:
2014-06-14
Description:
Accurate chromosome segregation during mitosis requires the physical separation of sister chromatids before nuclear envelope reassembly (NER). However, how these two processes are coordinated remains unknown. Here, we identified a conserved feedback control mechanism that delays chromosome decondensation and NER in response to incomplete chromosome separation during anaphase. A midzone-associated Aurora B gradient was found to monitor chromosome position along the division axis and to prevent premature chromosome decondensation by retaining Condensin I. PP1/PP2A phosphatases counteracted this gradient and promoted chromosome decondensation and NER. Thus, an Aurora B gradient appears to mediate a surveillance mechanism that prevents chromosome decondensation and NER until effective separation of sister chromatids is achieved. This allows the correction and reintegration of lagging chromosomes in the main nuclei before completion of NER.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Afonso, Olga -- Matos, Irina -- Pereira, Antonio J -- Aguiar, Paulo -- Lampson, Michael A -- Maiato, Helder -- New York, N.Y. -- Science. 2014 Jul 18;345(6194):332-6. doi: 10.1126/science.1251121. Epub 2014 Jun 12.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Chromosome Instability and Dynamics Laboratory, Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal. ; Chromosome Instability and Dynamics Laboratory, Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal. Center for Mathematics, Universidade do Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal. ; Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA. ; Chromosome Instability and Dynamics Laboratory, Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal. Cell Division Unit, Department of Experimental Biology, Faculdade de Medicina, Universidade do Porto, Alameda Prof. Hernani Monteiro, 4200-319 Porto, Portugal. maiato@ibmc.up.pt.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24925910" target="_blank"〉PubMed〈/a〉
Keywords:
*Anaphase
;
Animals
;
Aurora Kinase B/antagonists & inhibitors/genetics/*metabolism
;
Cell Line
;
Cell Line, Tumor
;
Chromosome Segregation/genetics/*physiology
;
Drosophila
;
*Feedback, Physiological
;
Humans
;
Nuclear Envelope/genetics/*metabolism
;
Protein Phosphatase 1/metabolism
;
Protein Phosphatase 2/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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