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  • 1
    ISSN: 1423-0445
    Keywords: Key words.Ostrinia latipennis– sex pheromone – (E)-11-tetradecenol – Lepidoptera – Crambidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary. Extracts from the sex pheromone gland of Ostrinia latipennis (Lepidoptera: Crambidae) were analyzed by gas chromatography-electroantennographic detection (GC-EAD) and GC-mass spectrometry. Only an EAD-active compound was detected in the extract, and it was identified as (E)-11-tetradecenol (E11-14:OH). In a wind-tunnel bioassay, E11-14:OH elicited a series of mate finding behaviors from males, although it was far less active than virgin females and crude extract of the pheromone gland. The attractiveness of E11-14:OH to O. latipennis males was confirmed by field trapping experiments. Based on these findings, we concluded that E11-14:OH, which is novel to the genus Ostrinia, is a major component of the sex pheromone in O. latipennis. The significance of the use of alcohol in place of the usual acetates in Ostrinia is discussed in relation to the pheromone biosynthesis system.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 75 (1995), S. 189-191 
    ISSN: 1570-7458
    Keywords: Helicoverpa assulta ; sex pheromone ; PBAN ; oriental tobacco budworm moth ; (Z)-9-hexadecenal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 89 (1998), S. 281-287 
    ISSN: 1570-7458
    Keywords: Ostrinia zaguliaevi ; sex pheromone ; GC-EAD ; (Z)-9-tetradecenyl acetate ; (Z)-11-tetradecenyl acetate ; (E)-11-tetradecenyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The sex pheromone blend of the butterbur borer, Ostrinia zaguliaevi (Lepidoptera: Pyralidae) was analyzed by means of gas chromatography-electroantennographic detection (GC-EAD), GC-mass spectrometry and a series of wind-tunnel bioassays. Four EAD-active compounds were detected in the female sex pheromone gland extract, and these were identified as tetradecyl acetate (14:OAc), (Z)-9-tetradecenyl acetate (Z9-14:OAc), (E)-11-tetradecenyl acetate (E11-14:OAc) and (Z)-11-tetradecenyl acetate (Z11-14:OAc). The average amounts ± s.d. of the four compounds in a single sex pheromone gland were 7.9±3.7 ng, 10.1±3.2 ng, 1.1±0.5 ng and 11.6±5.1 ng, respectively. In a wind-tunnel bioassay, the ternary blend of Z9-, E11- and Z11-14:OAc at a ratio found in the sex pheromone gland (45:5:50) elicited the same behavioral responses from the males as did virgin females and pheromone gland extract. Removal of any single compound from the ternary blend significantly diminished the pheromonal activity, whereas addition of 14:OAc to the ternary blend had no effect on the males' behavioral responses. Therefore, it was concluded that the sex pheromone blend of O. zaguliaevi is composed of Z9-14:OAc, E11-14:OAc and Z11-14:OAc at a ratio of 45:5:50.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 91 (1999), S. 237-244 
    ISSN: 1570-7458
    Keywords: host plant range ; sex pheromone ; Ostrinia furnacalis ; Ostrinia latipennis ; Ostrinia nubilalis ; Ostrinia orientalis ; Ostrinia palustralis ; Ostrinia scapulalis ; Ostrinia zaguliaevi ; Ostrinia zealis ; Lepidoptera ; Pyralidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To contribute to the understanding of the genus Ostrinia (Lepidoptera; Pyralidae) in Japan, we collected larvae of Ostrinia spp. from known host plants and plants not recorded as hosts, and we examined the morphology and sex pheromones of the adults obtained. Consequently, the host plant ranges of the 7 Ostrinia spp. in Japan were clarified, and the sex pheromones of the 5 species O. scapulalis, O. zealis, O. zaguliaevi, O. palustralis and O. latipennis were identified in addition to that of the Asian corn borer O. furnacalis. The phylogenetic relationships of Japanese Ostrinia spp., with reference to the European corn borer O. nubilalis, are discussed based on these findings and results of molecular phylogenetic analyses.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 86 (1998), S. 313-318 
    ISSN: 1570-7458
    Keywords: Ostrinia palustralis ; sex pheromone ; identification ; (E)-11-tetradecenyl acetate ; (Z)-11-tetradecenyl acetate ; Lepidoptera ; Pyralidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract By means of gas chromatography with electroantennographic detection, gas chromatography-mass spectrometry and a series of bioassays, (E)-11-tetradecenyl acetate (E11-14:OAc) and (Z)-11-tetradecenyl acetate (Z11-14:OAc) at a ratio of 99:1 were identified as female sex pheromone components of Ostrinia palustralis. The average amounts of E11- 14:OAc and Z11-14:OAc in a single sex pheromone gland were 37.2±24.4 ng and 0.3±0.2 ng, respectively. In a wind-tunnel bioassay, the binary blend of E11- and Z11-14:OAc elicited the same male behavioral responses as did virgin females.
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  • 6
    ISSN: 1570-7458
    Keywords: Cnaphalocrocis medinalis ; rice leaffolder ; Lepidoptera ; Pyralidae ; sex pheromone ; geographical variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sex pheromone components of the Japanese rice leaffolder moth, Cnaphalocrocis medinalis Guenée (Lepidoptera: Pyralidae) were identified from ovipositor extracts of virgin females as (Z)-11-octadecenal, (Z)-13-octadecenal, (Z)-11-octadecen-1-ol and (Z)-13-octadecen-1-ol at a ratio of 11:100:24:36 by GC-EAD, GC, GC-MS. The total amount was estimated to be ca.0.9 ng/female. Field bioassays in Kagoshima, Japan, showed that the two aldehydes are essential for male attraction and the alcohols may have a synergistic effect on the aldehydes. A rubber septum containing 0.9 mg of the four components at the natural ratio was shown to be an effective lure for monitoring this pest in Japan. The above four components are quite different from the sex pheromone components reported previously for the same species of either Philippine or Indian origin; components were shown to be (Z)-11-hexadecenyl acetate and (Z)-13-octadecenyl acetate at a ratio of 98:2 in the Philippine blend and 1:10 in the Indian blend. Furthermore, in the field tests in Japan, neither the Philippine blend nor the Indian blend showed any attractive activity, while the Japanese blend attracted significant numbers of male moths. These results suggest that there are remarkable geographical variations in the sex pheromone composition of this species or there are several distinct species using different sex pheromone blends.
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  • 7
    ISSN: 1573-1561
    Keywords: Ostrinia scapulalis ; sex pheromone ; (Z)-11-tetradecenyl acetate ; (E)-11-tetradecenyl acetate ; GC-EAD ; field trapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract By means of gas chromatography with electroantennographic detection (GC-EAD), gas chromatography–mass spectrometry (GC-MS), and a series of bioassays, (Z)-11-tetradecenyl acetate (Z11–14:OAc) and (E)-11-tetradecenyl acetate (E11–14:OAc) at a ratio of 100:3 were identified as the female sex pheromone of the adzuki bean borer,Ostrinia scapulalis. The average amounts ofZ11–14: OAc andE11–14:OAc in a single sex pheromone gland were 6.6 ± 2.4 ng and 0.2 ± 0.1 ng, respectively. In a wind-tunnel bioassay, the binary blend ofZ11- andE11–14:OAc elicited almost the same male behavioral responses as did virgin females and sex pheromone gland extract. In field trapping experiments, rubber septa impregnated with the binary blend (50 μg/septum) attracted more males than virgin females. The sex pheromone ofO. scapulalis thus turned out to be similar to that of theZ-type European corn borer,O. nubilalis, in both components and their ratio.
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  • 8
    ISSN: 1573-1561
    Keywords: Helicoverpa assulta ; sex pheromone ; calling ; diel periodicity ; Lepidoptera ; Noctuidae ; (Z)-9-hexadecenal ; hexadecanal ; (Z)-11-hexadecenal ; (Z)-9-hexadecenyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Both calling behavior and titer of (Z)-9-hexadecenal (Z9-16: Al), the major sex pheromone component ofHelicoverpa assulta, in pheromone glands showed distinct diel periodicity, and these two were synchronous. Calling was most actively performed and the pheromone titer reached a maximum from 2 to 6 h after lights-off. During photophase, no calling was shown and only a relatively small amount of Z9-16:A1 was detected. However, there was a time lag of a few days between peak calling activity and maximum pheromone titer. The pheromone titer was maximal from age 1 day to age 5 days and thereafter decreased while calling was most actively performed after age 3 days. Titers of three minor components, hexadecenal, (Z)-11-hexadecenal, and (Z)-9-hexadecenyl acetate, showed similar daily fluctuation patterns to that of Z9-16:Al, but relative to the titer of Z9-16:Al, the titer of the two aldehyde components remained relatively constant whereas that ofZ9-16:Ac increased in the late scotophase.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of chemical ecology 25 (1999), S. 105-115 
    ISSN: 1573-1561
    Keywords: Sex pheromone ; legume pod borer ; Maruca vitrata ; Pyralidae ; Lepidoptera (E,E)-10,12-hexadecadienal ; attraction ; geometrical isomer ; isomeric purity ; antagonistic effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract An EAD-active component in a female abdominal tip extract of the legume pod borer, Maruca vitrata (Fabricius) (= M. testulalis), was identified as (E,E)-10,12-hexadecadienal (E10,E12–16:Ald). In laboratory bioassays, the purified synthetic E10,E12–16:Ald with 99% isomeric purity had attractancy to male moths equal to the crude extract. However, the unpurified chemical with 92% isomeric purity did not show any attractancy. Addition of 0.1 ng EZ isomer or 1 ng of EZ, ZE, or ZZ isomers of 10,12–16: Ald to 1 ng of the purified synthetic pheromone antagonized its attractancy as did addition of each isomer to 1 female equivalent of the crude extract. These results suggest that it is necessary to develop formulation and release techniques that maintain extremely high isomeric purity of the synthetic pheromone for male trapping in the field.
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  • 10
    ISSN: 1573-1561
    Keywords: Kairomone ; oviposition stimulant ; amino acid ; parasitoid ; Ascogaster reticulatus ; Hymenoptera ; Braconidae ; Adoxophyes sp. ; Lepidoptera ; Tortricidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Several components of an internal kairomone were identified inside eggs of the host,Adoxophyes sp. (Lepidoptere: Tortricidae), that releases egg deposition of the egg-larval parasitoid,Ascogaster reticulatus Watanabe (Hymenoptera: Braconidae). Pupal hemolymph with the same activity as an internal host egg kairomone was used as a convenient test sample. Heat-treated pupal hemolymph was chromatographed on a Sephadex G-25 column. Each fraction was bioassayed and reacted with ninhydrin. The active fractions were ninhydrin-positive. Each fraction was placed onto an araino acid analyzer, which showed that the amino acids were most abundant in active fractions. Among 22 amino acids, alanine, arginine, glycine, histidine, isoleucine, leucine, methionine, proline, serine, tryptophan, and valine were active. The mixture of these active amino acids was as active as the egg-mass homogenate at the same ratio and concentration, suggesting that the most important component as the kairomone in a host egg is the mixture of several amino acids.
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