Abstract
Small nucleolar RNAs (snoRNAs) are trans‐acting factors involved in maturation of rRNA and have been classified into Box C/D and Box H/ACA families. Most of the snoRNAs occur as ribonucleoprotein complexes with snoRNA‐associated proteins (snoRNPs). All Box C/D snoRNAs in yeast form complexes with Nop1p, Nop56p and Nop58p. Similarly, it has been reported that Box H/ACA‐containing snoRNAs form complexes with yeast Gar1p. Nop56p and Nop58p homologs have been described in several species. Here we report the isolation and molecular characterization of the Dnop56 genes from D. melanogaster and D. subobscura which show a very similar structure. Drosophila Nop56p proteins contain lysine‐rich regions at their carboxy‐terminus, and show a high degree of similarity to other Nop56p proteins from different organisms. Phylogenetic relationships among these proteins and other snoRNPs have been established.
Similar content being viewed by others
References
Adams, M.D., S.E. Celniker, R.A. Holt, C.A. Evans, J.D. Gocayne et al., 2000. The genome sequence of Drosophila melanogaster. Science 287: 2185–2195.
Balakin, A.G., L. Smith & M.J. Fournier, 1996. The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions. Cell 86: 823–834.
De Frutos, R., K. Kimura & K. Peterson, 1990. In situ hybridization of Drosophila polytene chromosomes with digoxigenin-dUTP labeled probes. Methods Mol. Cell. Biol. 2: 32–36.
Gautier, T., T. Berg's, D. Tollervey & E. Hurt, 1997. The nucleolar KKE/D-repeated proteins Nop56p and Nop58p interact with Nop1 and are required for ribosome biogenesis. Mol. Cell. Biol. 17: 7088–7098.
Hooper, J.E., M. Pérez-Alonso, J.R. Bermingham, M. Prout, B.A. Rocklein, M. Wagenbach, J.E. Edstrom, R. de Frutos & M.P. Scott, 1992. Comparative studies of Drosophila Antennapedia genes. Genetics 132: 453–469.
Kumar, S., K. Tamura & M. Nei, 1993. MEGA: Molecular Evolutionary Genetic Analysis, version 1.02. The Pennsylvania State University, University Park, PA16802.
Maxwell, E.S. & M.J. Fournier, 1995. The small nucleolar RNAs. Annu. Rev. Biochem. 35: 897–934.
Munté, A, M. Aguadé & C. Segarra, 1997. Divergence of the yellow gene between Drosophila melanogaster and D. subobscura: recombination rate, codon bias and synonymous substitutions. Genetics 147: 165–175.
Newman, D.R., J.F. Jun, G.M. Shanab & E.S. Maxwell, 2000. Box C/D snoRNA-associated proteins: two pairs of evolutionary ancient proteins and possible links to replication and transcription. RNA 6: 861–879.
Omer, A.D., T.M. Lowe, A.G. Russell, H. Ebhardt, S.R. Eddy & P.P. Dennis, 2000. Homologs of small nucleolar RNAs in Archaea. Science 288: 517–522.
Paricio, N., M. Pérez-Alonso, M.J. Martínez-Sebastián & R. de Frutos, 1991. P sequences of Drosophila subobscura lack exon 3 and may encode a 66 kd repressor-like protein. Nucleic Acids Res. 19: 6713–6718.
Powel, J.R., 1997. Phylogenetics: Genus Drosophila, pp. 267–298. in: Progress and prospects in evolutionary biology, the Drosophila model. Oxford University Press, New York.
Saitou, N., & M. Nei, 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406–425.
Smith, C.M. & J.A. Steitz, 1997. Sno storm in the nucleolus: New roles for myriad small RNPs. Cell: 669–672.
Schimmang, T., D. Tollervey, H. Kern, R. Frank & E.C. Hurt, 1989. A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNAs and is essential for viability. EMBO J. 8: 4015–4024.
Terol, J., M. Pérez-Alonso & R. De Frutos, 1995. Molecular characterization of the zerknüllt region of the Antennapedia complex of D. subobscura. Chromosoma 103: 613–624.
Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin & D.G. Higgins, 1997. The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25: 4876–4882.
Venema, J. & D. Tollervey, 1995. Processing of pre-ribosomal RNA in S. cerevisiae. Yeast. 11: 1629–1650.
Venema, J. & D. Tollervey, 1999. Ribosome synthesis in S. cerevisiae. Annu. Rev. Genet. 33: 261–311.
Vorbrüggen, G., S. Önell & H. Jäckle, 2000. Restricted expression and subnuclear localization of the Drosophila gene Dnop5, a member of the Nop/Sik family of the conserved rRNA processing factors. Mech. Dev. 90: 305–308.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
García‐Planells, J., Paricio, N., Palau, F. et al. Dnop56, a Drosophila gene homologous to the yeast nucleolar NOP56 gene. Genetica 109, 275–282 (2000). https://doi.org/10.1023/A:1017596312331
Issue Date:
DOI: https://doi.org/10.1023/A:1017596312331