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  • Artikel  (58)
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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 30 (1981), S. 151-156 
    ISSN: 1570-7458
    Schlagwort(e): Xyleborus ferrugineus ; pupae ; ecdysteroids ; pharate adult ; radioimmunoassay ; Coleoptera ; Scolytidae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Der Ecdysteroidtiter weiblicher Puppen von Xyleborus ferrugineus (Fabr.) wurde geschätzt, indem ganze Tiere homogenisiert und radioimmunologisch untersucht wurden. Ein ausgeprägtes Maximum an Ecdysteroiden wurde bei 36 Stunden Puppenent-wicklung beobachtet (743 pg/mg Körpergewicht). Der Titer nahm ab auf 299 pg/mg im Pharatstadium und auf 193 pg/mg unmittelbar vor Schlüpfen der Adulten. Qualitative Studien mit HPLC ergaben in frischen Puppen ein Verhältnis von 3:1 Ecdyson zu 20-Hydrooxyecdyson. Pharatstadien enthielten vor allem 20-Hydrooxyecdyson. Das beobachtete einzige Maximum im Titer stimmt überein mit den Resultaten bei andern untersuchten Coleopteren.
    Notizen: Abstract Ecdysteroid titers were estimated on the whole body homogenates of Xyleborus ferrugineus (Fabr.) female pupae during development by radioimmunoassay. A distinct peak of ecdysteroids was observed at 36-hr pupal development (743 pg/mg body wt). Titer declined to 299 pg/mg by the pharate adult stage and to 193 pg/mg body wt just before adult emergence. Qualitative studies by HPLC revealed a ratio of 3:1 ecdysone to 20-hydroxyecdysone in the initial pupal stage. Pharate adults had mainly 20-hydroxyecdysone. The observed single peak in ecdysteroid titer agrees with findings in other studied coleopteran species.
    Materialart: Digitale Medien
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 190 (1981), S. 118-122 
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Aggregation ; Lectins ; Cell surface ; Embryo-derived cell line
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary In this paper we describe the aggregation of cells from embryo-derived cell lines ofDrosophila, measured by examining the ability of single cells to adhere to one another when suspended in culture medium and swirled on a rotary shaker. Using this method we demonstrated the presence of receptors for Concanavalin A, soybean agglutinin, and possibly wheat germ agglutinin on the surface of Schneider's line-2 cells. Our work provides basic descriptive and background information for further studies onDrosophila cells, including those isolated from imaginal discs.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 190 (1981), S. 297-300 
    ISSN: 1432-041X
    Schlagwort(e): Myosins ; Drosophila ; muscle
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Electrophoresis of myosin extracts from larvae and adult tissues ofDrosophila melanogaster under non-dissociating conditions indicate that two of the bands seen are myosins. They stain for Ca2+ ATPase activity and when cut and re-run under dissociating conditions are found to contain a myosin heavy chain that co-migrates with rabbit skeletal muscle myosin heavy chain. One of the forms of myosin seen is found primarily in extracts from the leg. The other is common to the adult fibrillar flight muscles and the larval body wall muscles. The electrophoretic evidence for two myosin types is strengthened by the histochemical demonstration of two myofibrillar ATPases on the basis of their lability to acid or alkali preincubation. The myofibrillar ATPase in the leg and the Tergal Depressor of the Trochanter (TDT) are shown to be relatively acid labile and alkali stable. The larval body wall muscles and the adult fibrillar flight muscles have an ATPase which is acid stable and alkali labile. This distribution of the two myofibrillar ATPase coincides with that predicted by electrophoresis of extracts from whole tissue and also locates the two myosins to specific muscle types.
    Materialart: Digitale Medien
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 190 (1981), S. 185-190 
    ISSN: 1432-041X
    Schlagwort(e): Wound healing ; Regeneration ; Imaginal discs ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary When complementary fragments of an imaginal disc ofDrosophila are cultured for several days prior to metamorphosis, usually one fragment will regenerate while the other will duplicate. It has been proposed that wound healing plays an important part in disc regulation (French et al. 1976; Reinhardt et al. 1977) by initiating cell proliferation and determining the mode of regulation. We tried to delay the wound healing process by leaving a region of dead cells between the wound edges. In “06” fragments (Bryant 1975a) wound healing has occurred after 1–2 days of culture and the regeneration of missing structures after 2–4 days of culture. We observed that leaving a region of dead cells between the wound edges delays both wound healing and the regeneration of missing structures by 2 days. When disc fragments are cultured in female abdomens and then exposed to3H-thymidine to label replicating cells, then the label is found to be localised around the wound. We observed that delaying wound healing does not delay this localisation of labelled nuclei indicating that wound healing may not be required to initiate DNA replication.
    Materialart: Digitale Medien
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 190 (1981), S. 156-160 
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Imaginal discs ; Transdetermination ; Homeosis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The transdetermination capacities of leg discs ofDrosophila melanogaster were examined by mechanically disrupting and kneading whole discs from late third instar larvae and by culturing the resulting tissue mass for 10–14 days in adult female abdomens where the cells continued to divide. The grown implants were then dissected from the abdomens and injected into third instar larvae to undergo metamorphosis. After this treatment, prothoracic leg discs ofDrosophila melanogaster transdetermined with a high frequency (59% of all implants) to wing. Mesothoracic leg discs also transdetermined to wing, but at a very low frequency (4%). Metathoracic leg discs exhibited the same low frequency of transdetermination (4%), but in this case the direction of transdetermination was to haltere (Table 1,D. melanogaster). Very similar results were obtained with leg discs ofDrosophila nigromelanica (Table 1,D. nigromelanica), showing that the peculiar behavior of the three leg discs is not unique forDrosophila melanogaster. The homeotic mutation Polycomb (Pc 3) which partially transforms meso- and metathoracic legs into prothoracic legs did not significantly increase the frequencies of transdetermination in these leg dises and had clearly no effect on the direction of transdetermination (Table 1).
    Materialart: Digitale Medien
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 190 (1981), S. 11-21 
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Imaginal discs ; Ecdysteroid ; Lethal mutant ; Morphogenesis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Imaginal disc development in the non-pupariating lethall(1)npr-1, a mutant that maps to an ecdysone early puff site, is studied in situ, in vitro and in transplanted discs. Disc development is slightly abnormal from the middle of the third instar with severe abnormalities appearing after the rise in 20-hydroxyecdysone that triggers metamorphosis. The mutant discs only partly evaginate and do not undergo any of the detailed morphological changes characteristic of metamorphosis. Treatment of the mutant dises in vitro with colcemid and trypsin facilitates evagination but the appendages remain morphologically abnormal. A number of differentiative processes occur in mutant discs in situ and in discs transplanted into wild type hosts in spite of the absence of normal morphogenesis. Implications of the observations for normal disc development are discussed. Possible modes of action of thel(1)npr-1 gene are also discussed in light of the observation that the mutant gene maps to a locus which is thought to have a regulatory function in development.
    Materialart: Digitale Medien
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 190 (1981), S. 132-138 
    ISSN: 1432-041X
    Schlagwort(e): Maternal effect mutant ; Homeotic-mutants ; Pattern formation ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The temperature sensitive mutationfs(l)h is characterized at the restrictive temperature of 29°C by both a maternal effect responsible for the early embryonic lethality and pupal zygotic lethality. The two phenotypes are inseparable and map at a short deletion in the X chromosome (7Dl, 7D5-6). At semipermissive temperatures, hemizygous mutant females produce adults with morphological defects, such as organ deficiencies and homeotic transformations of haltere to wing and third leg to second leg. These defects depend on the maternal genotype and are governed by an early temperature sensitive period, which covers the end of oogenesis and the first hours of embryogenesis. Furthermore, this maternal effect mutation interacts with some dominant mutations of the bithorax system. These properties suggest thatfs(l)h is somehow involved in segmental determination.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 190 (1981), S. 1-10 
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Grandchildless ; Pole cells ; ts-mutant ; Cytoplasmic determinant
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Two temperature-sensitive sex-linkedgrandchildless (gs)-like mutations (gs(1)N26 andgs(1)N441) were induced by ethylmethane sulphonate inDrosophila melanogaster. They complemented each other and mapped at two different loci (1−33.8±0.7 forgs(1)N26 and 1−39.6±1.7 forgs(1)N441), which were not identical to those of any of thegs-like mutants reported in earlier work. Homozygous females of the newly isolated mutants produced eggs that were unable to form pole cells and developed into agametic adults. Competence of the embryos to form pole cells was not restored by wild-type sperm in either mutant; that is, the sterility caused by these mutations is controlled by a maternal effect. Fecundity and fertility ofgs(1)N26 females were low, and their male offspring showed a higher mortality than that of female offspring, causing an abnormal sex ratio. The frequency of agametic progeny was 93.1% and 55.8%, when the female parents were reared at 25° C and 18° C, respectively. In eggs produced by thegs(1)N26 females reared at 25° C, the migration of nuclei to the posterior pole was abnormal, and almost no pole cell formation occurred in these egg. Furthermore, half of these eggs failed to cellularize at the posterior pole. When the females were reared at 18° C, almost all of the eggs underwent complete blastoderm formation, and in half of these blastoderm embryos normal pole cells were formed. In the other mutant,gs(1)N441, the fecundity and fertility of the females were normal. The agametic frequency in the progeny was 70.8% and 18.6% when the female parents were reared at 25° C and 18° C, respectively. In the eggs laid by females reared either at 25° C or at 18° C, the migration of nuclei to the periphery and cellularization proceeded normally; nevertheless, in the majority of the embryos no pole cell formation occured at the stage when nuclei penetrated into the periplasm. When the females were reared at 18° C, some of the embryos from these females formed some round blastoderm cells with cytologically recognizable polar granules and nuclear bodies, which are attributes of pole cells. The temperature sensitive period ofgs(1)N441 was estimated to extend from stage 9 to 13 of King's stages of oogenesis.
    Materialart: Digitale Medien
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  • 9
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 190 (1981), S. 308-312 
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; ts-Suppressor mutant ; Glue proteins ; Intermolt puffs ; Electrophoresis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The l(1)su(f)ts67g mutation has been shown to suppress the developmentally regulated expression of glue protein genes at 30°C. Transferring mutant larvae to the restrictive temperature before the end of the second larval instar results in the absence or extreme reduction of glue protein synthesis while general protein synthesis is unaffected. At the same time, the three glue protein correlated chromosomal regions 3C, 25B, and 68C continue to show prominent puffs. The results suggest that the mutation may be affecting the processing or translatability of specific mRNAs rather than the translational machinery itself.
    Materialart: Digitale Medien
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  • 10
    ISSN: 1432-041X
    Schlagwort(e): Mollusca ; Cleavage ; Junctions ; Interaction ; Development
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary In early cleavage stages ofLymnaea stagnalis, three kinds of intercellular junctions could be distinguished up to the sixth cleavage: intermediate, septate and gap junctions. The first two form “junctional belts” located on the cell border at the periphery of the embryo. For the purpose of our study we were most interested in gap junctions as they are alleged to be structures that allow cell-to-cell communication. Gap junctions first appear at the four cell stage. Up to the sixth cleavage no difference in the distribution pattern could be found between and within each of the four quadrants of the embryo. Some of the cell tiers along the animal-vegetal axis lack gap junctions either between the blastomeres within the tier or between the blastomeres from adjacent tiers. All gap junctions observed in freeze fracture replicas show plaques with an irregular IMP pattern. The average IMP diameter measures 12 nm (SD±2 nm). In stages fixed after the fifth cleavage, gap junctions are found between micromeres at the animal pole and the central 3D macromere. This is in agreement with the presumed interaction between these cells at this stage. The possibility of a transition of non-functional into functional gap junctions after the fifth cleavage is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 11
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 190 (1981), S. 301-303 
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Geographic strains ; Chorion genes ; Electrophoretic variants
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Further IF screening ofDrosophila melanogaster geographic strains has revealed a variant of the s19 major chorion protein. Developmental analysis of F1 hybrids indicates that the source of the variation is found in the structural gene for this protein. The linkage group of the variant gene was determined to be the third, and the gene was localized by several methods of recombination analysis. The s19 gene was found to be tightly linked to thesepia locus, as had been previously found for the s18 gene (Yannoni and Petri 1980). Lack of recombination between the s19 and s18 genes in double heterozygotes suggested that these two genes are within 0.3 map units of each other. Although more precise localization of the s19 gene failed, the s18 gene could be more specifically located to the right ofsepia, betweensepia andhairy. Contrary to our prediction (ibid.), the s19 and s18 genes have been found to be tightly linked in spite of the fact that they display somewhat different developmental stage specificity.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 12
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 190 (1981), S. 370-373 
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Homoeotic mutants ; Ventral cord
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary We describe a set of cells in the central nervous system of theDrosophila embryo which are restricted to the thoracic ganglia in the wildtype. Taking these cells as indication of thoracic identity, we find that the ventral cord of embryos homozygous mutant for different bithorax functions and for Polycomb undergoes homoeotic transformations equivalent to those observed in the larval cuticle.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 13
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 190 (1981), S. 33-39 
    ISSN: 1432-041X
    Schlagwort(e): Ceratitis capitata ; Major haemolymph proteins ; Development ; Fat body ; Secretion
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The accumulation of major haemolymph proteins (a group of proteins immunologically related to Calliphorin) their biosynthesis in vivo and in organ culture as well as their secretion, has been studied during the late larval stages and white pupae of the Mediterranean fruit flyCeratitis capitata. The accumulation of major haemolymph proteins in the haemolymph, shows a twenty fold increase from the 4-day old larvae to the white pupae stage, while in the fat body there is only a seven fold increase. It is evident from the in vivo and organ culture studies, that the major haemolymph proteins are synthesized during the late larval stage and their synthesis declines abruptly during the stage of white pupae. It seems also that each polypeptide has its own characteristic developmental kinetics of synthesis. The major haemolymph proteins are synthesized in the fat body and are very quickly secreted into the haemolymph.
    Materialart: Digitale Medien
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  • 14
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 129 (1981), S. 349-352 
    ISSN: 1432-072X
    Schlagwort(e): Rhizobium ; Bacteroids ; Viability ; Development
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Viability of Rhizobium leguminosarum bacteroids isolated from Pisum sativum was determined after sucrose gradient centrifugation of the bacteroids resulting in separation according to developmental stage. The results suggest that past a critical stage of development bacteroids lose viability. Similar experiments with R. japonicum bacteroids having a lower degree of morphological differentiation showed an appreciably higher viability in all gradient fractions. The results support the hypothesis that bacteroid morphology and viability is dependent on the nature of—and length of exposure to—the host plant cytoplasm.
    Materialart: Digitale Medien
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  • 15
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 19 (1981), S. 567-583 
    ISSN: 1573-4927
    Schlagwort(e): aldehyde oxidase ; Drosophila ; evolution ; gene regulation ; isozymes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract At least four enzymes contribute to histochemically, electrophoretically, or spectrophotometrically detectable aldehyde oxidase (AO) activity in Drosophila melanogaster. The one we designate AO-1 contributes the majority of activity measured in extracts of whole flies. Pyridoxal oxidase (PO) is also a broad range AO. It is prominent only in midgut and Malpighian tubules, where it apparently accounts for a substantial fraction of total AO activity. The tissue distributions of these enzymes are clearly disparate despite close linkage of their structural loci and parallel dependence on the mal, lxd, and cin loci. A similarly related enzyme, xanthine dehydrogenase (XDH), is detected as an AO only in electrophoretic gels. A fourth broad range AO, not dependent on mal, lxd, and cin, is confined to the ejaculatory bulb. A similar array of AO isozymes is present in phylogenetically distant Drosophila species.
    Materialart: Digitale Medien
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  • 16
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 19 (1981), S. 75-85 
    ISSN: 1573-4927
    Schlagwort(e): dipeptidases ; variation ; allozymes ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Genetic variation at three dipeptidase loci (Dip-A, Dip-B, and Dip-C) in Drosophila simulans was analyzed by starch gel electrophoresis. Dip-A was found to be polymorphic in four populations, while Dip-B and Dip-C were found to be polymorphic in one. The numbers of different alleles found at each respective locus were: Dip-A, two; Dip-B, two; and Dip-C, three. Dip-A was genetically mapped at 57.9 on the second chromosome, and Dip-B and Dip-C at 80.9 and 87.9 on the third chromosome, respectively. Neither Dip-B nor Dip-C has been mapped in D. melanogaster because both loci are apparently monomorphic. Their map positions in D. simulans with respect to flanking markers whose homologous genes have been cytogenetically localized in D. melanogaster suggested that they might be mapped cytogenetically by using available deficiencies in D. melanogaster. Accordingly, by the construction of interspecific hybrids which carried deficiencies of melanogaster and an allele of simulans with a mobility different from that of the fixed melanogaster allele, Dip-B and Dip-C were localized between 87F12-14 and 88C1-3 and between 87B5-6 and 87B8-10, respectively, in the salivary gland chromosomes of D. melanogaster. The similarity between these two species is discussed on the basis of these findings.
    Materialart: Digitale Medien
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  • 17
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 19 (1981), S. 115-127 
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; dihydroorotate dehydrogenase ; pyrimidine biosynthesis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract A locus is described that controls levels of mitochondrial dihydroorotate dehydrogenase (EC 1.3.3.1) in Drosophila melanogaster. The effects of alleles of the locus, Dhod, are manifest in preparations from whole organisms as well as in partially purified mitochondrial preparations; however, other mitochondrial functions do not appear to be appreciably affected by Dhod genotypes. The locus maps near p in the proximal portion of the right arm of chromosome 3. Flies trisomic for a chromosome segment including that region display elevated enzyme levels, implying that an enzyme structural gene is in that vicinity. Furthermore, Dhod alleles are semidominant in heterozygotes, suggesting that the dosage-sensitive element detected in the trisomics is actually the Dhod locus. These findings are discussed relative to the role of dihydroorotate dehydrogenase in the de novo pyrimidine biosynthetic pathway and relative to other pathway mutants that have been described in Drosophila.
    Materialart: Digitale Medien
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  • 18
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 19 (1981), S. 311-320 
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; thermal stability of enzyme ; α-glycerophosphate dehydrogenase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Thermal stability of α-glycerophosphate dehydrogenase-1 (α-Gpdh-1) in nine Drosophila species was studied at pH's ranging from 6.4 to 8.5. This was done by measuring the changes in the activity of enzymes during the heat denaturation process. In addition to temperature, the rate of denaturation is highly dependent on the pH of the incubation buffer. The results of this study show that the thermal stability of enzyme molecules is different in different species. This holds true also in the species in which the enzymes have been found to be identical by other means. The differences between species of the Drosophila virilis group are discussed.
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  • 19
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 19 (1981), S. 321-331 
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; embryonic cells ; actin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract In a permanent cell line derived from Drosophila embryos, cytoplasmic actin is produced as an unstable precursor, which is subsequently converted to a stable form. This conversion results in a reduction in isoelectric point, with no apparent change in molecular weight. The conversion involves an enzymatic acetylation, and results in an insensitivity to aminopeptidase digestion, suggesting N-terminal blockage. Both the acetylated and unacetylated actins can participate in the assembly of F-actin, but with different efficiencies.
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  • 20
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 19 (1981), S. 411-419 
    ISSN: 1573-4927
    Schlagwort(e): half life ; Drosophila ; alcohol dehydrogenase ; enzyme stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract A rapid and accurate method of measuring the relative in vivo stability of Drosophila alcohol dehydrogenase is presented. The potential of this technique for examining posttranslational control of in vivo enzyme concentrations is discussed.
    Materialart: Digitale Medien
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  • 21
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 19 (1981), S. 421-430 
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; alcohol dehydrogenase ; adaptation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The effects of environmental 2-propanol on the in vivo properties of Drosophila alcohol dehydrogenase (E.C. 1.1.1.1.) are presented. Exposed flies were found to exhibit a significant decrease in ADH specific activity with a concomitant increase in the enzyme's relative in vivo stability and concentration. The possible adaptive significance of the observed responses is discussed.
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  • 22
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 19 (1981), S. 783-796 
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; amylase ; gene regulation ; trans effect
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Purified amylases from high- and low-activity variants of Drosophila melanogaster showed identical specific activities. Immunoelectrophoresis of crude larval homogenates showed severalfold differences between strains in the amounts of cross-reacting material. Control of amylase activity is “trans”-acting in heterozygotes between high- and low-activity variants. These results suggest the existence of polymorphic regulatory genes affecting the production levels of amylase protein in D. melanogaster.
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  • 23
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 19 (1981), S. 947-954 
    ISSN: 1573-4927
    Schlagwort(e): alcohol dehydrogenase ; Drosophila ; cryptic variants
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Thirty-five cryptic variant lines were used to examine the mechanisms involved in genetic modulation of alcohol metabolism in Drosophila. Late third-instar larval, preemergence pupal, and adult stages cultured at 18 and 28 C were examined. Spectrophotometric analyses for native alcohol dehydrogenase (ADH) activity and residual ADH activity after treatment with guanidine hydrochloride and heat were performed. Differential response of cryptic variants to treatment with the denaturants during development suggested that this variation may have an adaptive significance.
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  • 24
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; molybdoenzymes ; sulfite oxidase ; tungstate feeding
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Sulfite oxidase (sulfite: ferricytochrome c oxidoreductase; EC 1.8.2.1) has been detected in Drosophila melanogaster and some of its properties have been studied. In most respects this enzyme resembles the mammalian sulfite oxidases except for its molecular weight (148,000), which is somewhat higher than that of rat sulfite oxidase (116,000). Cytochrome c, potassium-ferricyanide, and oxygen can serve as electron acceptors in the oxidation of sulfite by the enzyme. Although definite evidence can be obtained only through the analysis of the pure enzyme, experiments involving tungstate feeding suggest that Drosophila sulfite oxidase is most probably a molybdoenzyme. Extracts of mal flies show normal levels of sulfite oxidase, whereas lxd flies have only 5–10% of the activity of wild type, and in cin flies the enzyme is apparently absent. While it is possible that the lxd and cin mutations are at some level responsible for the defective synthesis of a molybdenum-containing cofactor (supposed to be present in most molybdoenzymes), the evidence accumulated so far by several authors and the results of the present investigation argue against the involvement of a Mo cofactor in the multiple enzyme deficiencies observed in mal flies.
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    Standort Signatur Erwartet Verfügbarkeit
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  • 25
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 7 (1981), S. 39-48 
    ISSN: 1573-1561
    Schlagwort(e): Insect feeding deterrents ; antifeedants ; Pissodes strobi Peck ; Coleoptera ; Curculionidae ; Thuja plicata Donn
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The feeding deterrent activity of fractions from the foliage of western red cedar,Thujaplicata Donn, was studied in laboratory bioassays using the white pine weevil,Pissodes strobi Peck, as a test insect. The most active fraction was the volatile mixture that comprises the leaf oil of this tree species. Further fractionation of the leaf oil indicated feeding deterrent activity in the monoterpene hydrocarbon, thujone, and terpene alcohol fractions. When tested alone, both (−)-3-isothujone and (+)-3-thujone, which made up 75–88% and 5–10% of the leaf oil, respectively, deterred feeding by the weevils. Western red cedar leaf oil also showed antifeedant activity with the alder flea beetle,Altica ambiens (Le Conte), and served as an oviposition deterrent for the onion root maggot,Hylemya antiqua Meigen. The leaf oil, however, had no inhibitory effect on the feeding of the leaf roller,Epinotia solandriana L., and the red-backed sawfly,Eriocampa ovata L.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 26
    Digitale Medien
    Digitale Medien
    Springer
    Behavior genetics 11 (1981), S. 557-563 
    ISSN: 1573-3297
    Schlagwort(e): Drosophila ; photobehavior ; pupation site selection ; sibling species
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Psychologie
    Notizen: Abstract Drosophila melanogaster prefers to pupate in the dark, while its sibling species,D. simulans, prefers the light when the species are tested in isolation and when cultured and tested together. Reciprocal interspecific hybridizations were carried out and the F 1 individuals were tested. Progeny from the cross ofD. melanogaster females withD. simulans males chose pupations sites exactly intermediate between those of the two parental species, while the reciprocal-cross offspring preferred light pupation sites. The pupation site preferences (PSPs) of the hybrids are compatible with a sex-linked locus or loci influencing light-dependent PSP in this pair of species. Examination of light preferences of larvae prior to the late third instar demonstrates that these preference are highly specific, being restricted to the time just before pupation. During the first two larval instarsD. melanogaster is quite photopositive whileD. simulans is comparatively photoneutral. These differences in light-dependent behavior could aid in reducing competition between the two species.
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    Standort Signatur Erwartet Verfügbarkeit
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  • 27
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 59 (1981), S. 23-24 
    ISSN: 1432-2242
    Schlagwort(e): Genetic sexing ; Alcohol dehydrogenase ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary By incorporating ethanol (4% v/v) into the larval rearing medium of a specially constructed Drosophila melanogaster strain it was possible to produce only male adults; the female larvae died. In this strain, the male determining chromosome was linked with a positive Alcohol dehydrogenase (ADH) allele by a translocation. The females were homozygous for the null allele and hence sensitive to ethanol. This genetic sexing method is discussed in relation to its use in the genetic control of insects.
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    Standort Signatur Erwartet Verfügbarkeit
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  • 28
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 217 (1981), S. 65-77 
    ISSN: 1432-0878
    Schlagwort(e): Freeze-fracture ; Occluding junctions ; Enterocytes ; Development
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The architecture of occluding junctions during the differentiation of the mouse duodenum was studied in freeze-fractured material. Irregular zonulae occludentes (ZO) (Type I) are numerous during fetal life, and are characterized by their irregular width, and by the presence of basal open-ended extensions fused with the discontinuous basal strand of the ZO. Regular ZOs (Type II), typical of the adult villous epithelium, appear after Type I junctions by day 16 of gestation. Two patterns are distinguishable: in the first, parallel strands of ridges and furrows are found without crossing branches; in the second pattern, the junction zone is organized like a network of short branches forming various types of polygons. In fetal and adult mice fasciae occludentes (FO) (Type III) are present on the lateral cell membranes; in unfixed specimens particles are found in the furrows of the E-face and pits on the ridges of the P-face. In fixed tissues, the particles are aligned on the ridges of the P-face. These results indicate that fixation with glutaraldehyde modifies considerably the affinity of junctional particles toward the P-face during the fracture process. Moreover, the presence of numerous large FOs on the lateral cell membranes of enterocytes during late fetal life and in the adult, is possibly related to cell movement along the intestinal villi.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 29
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 216 (1981), S. 377-401 
    ISSN: 1432-0878
    Schlagwort(e): Lamina ganglionaris ; Locust ; Neurons ; Development ; Light and electron microscopy ; Golgi study
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The gross structure as well as the neuronal and non-neuronal components of the lamina ganglionaris of the locust Schistocerca gregaria are described on the basis of light- and electron-microscopical preparations of Golgj (selective silver) and ordinary histological preparations. The array of optic cartridges within the lamina neuropile — their order and arrangement — and the composition of the cartridges are described. There are six types of monopolar neurons: three whose branches reach to other cartridges and three whose branches are confined to their own cartridges. Retinula axons terminate either in the lamina or the medulla neuropiles. There are three types of centrifugal neurons, two types of horizontal neuron, as well as glia and trachea in the lamina neuropile. The development of the lamina neuropile is described in terms of developing monopolar and centrifugal axons, growing retinula fibres, and composition of the developing optic cartridges.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 30
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 217 (1981), S. 155-170 
    ISSN: 1432-0878
    Schlagwort(e): Adrenal medulla ; Development ; Acetylcholinesterase ; Small granule cells ; Ultramorphology ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The development of the rat adrenal medulla was studied at the ultrastructural level with particular emphasis placed on early discrimination of different catecholamine-storing cells. The first granule-containing cells, phaeochromoblasts, were seen at day 15 of gestation migrating into the anlage of the cortex. These cells were characterized by a few small granules (80–120 nm in diameter) and a high nuclear to cytoplasmic ratio. Presumably due to differentiation into chromaffin cells, they were no longer present after the eighth postnatal day. Maturation of phaeochromoblasts was indicated by an increase in number and size of their storage granules and a decrease in the nuclear to cytoplasmic ratio. Noradrenaline and adrenaline cell types were first clearly discernible at day 21 of gestation. Another cell type, a giant cell, was also recognized at this stage. In the adult animal, noradrenaline, two morphologically different types of adrenaline, and small granule-containing cells were observed. By applying acetylcholinesterase histochemistry, it was found that at day 17 of gestation a small population of granule-storing cells showed strong positive staining in the endoplasmic reticulum. In the adult animal this cell type was further characterized by small-storage granules. Other chromaffin cells began to show weak staining within the endoplasmic reticulum at day 19 of gestation. This staining appeared more frequently within adrenaline than noradrenaline cells. However, even in the adult animal many cells of both types were completely negative. It is concluded that acetylcholinesterase histochemistry is a useful method for early discrimination of small granule-containing cells in the developing rat adrenal medulla.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 31
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 215 (1981), S. 563-575 
    ISSN: 1432-0878
    Schlagwort(e): Antennal lobes ; Development ; Sexual dimorphism ; Periplaneta americana
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The postembryonic development of the antennal lobes of Periplaneta americana L. was examined with light- and electron-microscopical methods. There is no difference in the number of glomeruli and neurons in the antennal lobes of larval and adult animals. At hatching, the first larva already possesses the adult number of approximately 125 glomeruli and 500 to 560 deutocerebral neurons in the dorsolateral cell group of each antennal lobe. During postembryonic development the volume of the deutocerebral neurons increases three- to fourfold. The glomeruli of the first larva have about 7 % of the volume of the corresponding adult glomeruli. Since number, pattern, and size ratio of glomeruli (with the exception of the macroglomerulus) are constant in all larval stages and adult animals, it is possible to identify individual glomeruli. During the whole postembryonic development the ordinary glomeruli show a continuous volume increase, which parallels the increase in antennal sensory input. The macroglomerulus develops by way of special growth of two to four neuropil units, but not before the last three to four larval stages and only in males. Its growth precedes the formation of antennal pheromone receptors during the final molt; these receptors are known to project into the macroglomerulus. The development of the macroglomerulus in the last larval stages of the male may be caused by a genetically fixed growth program of specific deutocerebral neurons.
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    Standort Signatur Erwartet Verfügbarkeit
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  • 32
    Digitale Medien
    Digitale Medien
    Springer
    Protoplasma 108 (1981), S. 83-98 
    ISSN: 1615-6102
    Schlagwort(e): Development ; Etioplast ; Prolamellar body ; Protochlorophyll ; Saponin ; Thylakoid
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Etioplasts of dark grown plants contain a large paracrystalline prolamellar body (PLB) and, attached to this there are prothylakoid membranes (PTs). PLB-tubules inAvena are composed mainly of two saponins and include only a low percentage of other lipids, protochlorophyll(ide) and proteins. Following the development of etioplasts in darkness from the very beginning until plants loose turgescence one can observe marked changes in ultrastructure. In the early stage of development predominantly PTs are seen in small etioplasts. Wide-type PLBs are small. After eight days there is a well developed stage with the well-known big and highly crystalline PLBs, which are connected to many long PT-membranes. After 13 days the PLBs are not significantly changed, while number and length of PTs are strongly reduced. These morphological observations are quantified by measurements of PLB-area and PT-length per plastid section. Saponin content as a marker for PLB-tubules and protochlorophyll(ide)-content as a marker for PT-membranes were measured. Both methods of determination show in good agreement a peak of development for PTs around day 6–7, and for PLBs around 9–10. Beginning senescence affects PT-membranes and PChl(ide) strongly, while saponins resp. PLBs persist better. These results are presented in view of thylakoid formation during greening, starting from the different etioplast stages.
    Materialart: Digitale Medien
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  • 33
    ISSN: 1615-6102
    Schlagwort(e): Calcium-oxalate crystals ; Development ; Mucilage ; Opuntia ficus-indica ; Stereology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary In the succulent tissue ofOpuntia ficusindica development of calcium oxalate crystals was followed from the shoot apex to its base, using stereological analysis. The stereological parameters relating to the crystal cells were compared with those of mucilage cells, of the same tissue. A fairly monotonic relation between the stereological parameters of the two populations was noted. The possible physiological relation between the two cell populations is discussed.
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  • 34
    Digitale Medien
    Digitale Medien
    Springer
    Behavior genetics 11 (1981), S. 127-133 
    ISSN: 1573-3297
    Schlagwort(e): sexual isolation ; Drosophila ; isofemale strains ; isolation index ; mating propensity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Psychologie
    Notizen: Abstract Eight isofemale strains of the cosmopolitan speciesDrosophila immigrans derived from a single location in Melbourne, Australia, were crossed in all combinations to test for sexual isolation. Statistically significant sexual isolation occurred in 12 of 28 crosses, with one strain showing significant isolation from the other seven. There were significantly unequal male mating propensities (relative rates of mating) in 7 of the 28 crosses.
    Materialart: Digitale Medien
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  • 35
    Digitale Medien
    Digitale Medien
    Springer
    Behavior genetics 11 (1981), S. 145-151 
    ISSN: 1573-3297
    Schlagwort(e): Bruchidae ; Callosobruchus ; Coleoptera ; nonadditive inheritance ; oviposition behavior
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Psychologie
    Notizen: Abstract As a result of different feeding regimens, two laboratory populations of the beetleCallosobruchus maculatus Fab. developed different rates of oviposition. The behavior of the F1 hybrids cannot be explained with an additive model of inheritance. The unusual pattern of inheritance suggests a sex-related factor(s) and a two-factor interaction.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 36
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 7 (1981), S. 1-7 
    ISSN: 1573-1561
    Schlagwort(e): Japanese beetle ; Popillia japonica ; Coleoptera ; Scarabaeidae ; sex attractant ; survey lure ; phenethyl propionate ; eugenol ; synergism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract A combination of the synthetic sex attractant (R,Z)-5-(1-decenyl) dihydro-2(3H)-furanone with a 3∶7 mixture of phenethyl propionate (PEP) and eugenol (4-allyl-2-methoxphenol) caught significantly morePopillia japonica Newman than either the sex attractant or the mixture did alone. Also, the synthetic sex attractant captured significantly more males than the PEP-eugenol did during the period of heavy adult emergence of the beetles. The two lures were not significantly different in their attractancy to males about a week later and thereafter. A combination of PEP-eugenol and virgin females in the same trap late in the season also significantly increased beetle captures.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 37
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 7 (1981), S. 9-18 
    ISSN: 1573-1561
    Schlagwort(e): Dendroctonus brevicomis ; Ips paraconfusus ; Coleoptera ; Scolytidae ; Pinus ponderosa ; bark beetle ; exo-brevicomin ; frontalin ; myrcene ; verbenone ; ipsenol ; ipsdienol ; cis-verbenol ; attractants ; inhibition ; semiochemicals ; pheromones
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Dendroctonus brevicomis was attracted to a mixture of theIps paraconfusus pheromones, ipsenol,cis-verbenol, and ipsdienol at 10−9 g each/μl but was not attracted to these pheromones at higher and lower release rates.I. paraconfusus was not attracted to theD. brevicomis pheromonesexo-brevicomin, frontalin, and myrcene at any release rate tested. Increased release rates of a mixture of the three pheromones ofI. paraconfusus inhibited the attraction ofD. brevicomis to its synthetic pheromones. A mixture of ipsenol + ipsdienol orcis-verbenol alone failed to cause inhibition indicating that at least two of theI. paraconfusus pheromones are required to inhibit the response ofD. brevicomis. The pheromones ofD. brevicomis did not inhibit the attraction ofI. paraconfusus to its pheromones; however, verbenone was a potent inhibitor.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 38
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 7 (1981), S. 33-38 
    ISSN: 1573-1561
    Schlagwort(e): Coleoptera ; Bostrichidae ; Rhyzopertha dominica ; aggregation pheromone ; lesser grain borer ; grain insect pest
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Adult males of the lesser grain borer,Rhyzopertha dominica (F.), produce a pheromone that attracts both sexes. The volatiles from males collected on filter paper or Porapak-Q were attractive in two laboratory bioassays. The mating behavior is described and the function of the phen infested with the borer.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 39
    ISSN: 1573-1561
    Schlagwort(e): Primary attraction ; tree predisposition ; Dendroctonus ; Ips ; Gnathotrichus ; Pityophthorus ; Coleoptera ; Scolytidae ; Buprestidae ; Verticicladiella ; Pinus ponderosa
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Detection of weakened hosts from a distance by bark beetles through olfaction was investigated in field experiments. No significant numbers of Scolytidae were attracted to anaerobically treated pine bolts, stem disks, or sugar and ponderosa pine bark including phloem. Treatment of living trees with cacodylic acid induced attacks byDendroctonus brevicomis, D. ponderosae, Ips latidens, Gnathotrichus retusus, andPityophthorus scalptor, beginning two weeks after treatment. There was no significant difference between landing rates ofD. brevicomis andD. ponderosae on screened treated trees and screened controls. There was a significant increase in landing rates ofG. retusus andI. latidens, because both species had penetrated the screen and produced pheromones. Tree frilling alone did not increase the landing rate of bark beetles. Freezing of the lower trunk with dry ice did not increase significantly the landing rate ofD. brevicomis, D. ponderosae, G. retusus, orI. latidens on screened trees, whereas unscreened frozen trees were attacked by all four species. There was no significantly higher landing rate byD. brevicomis, D. ponderosae, I. paraconfusus, I. latidens, G. retusus, orHylurgops subcostulatus on screened trees evidencing symptoms of severe infection by the root pathogenVerticicladiella wagenerii, than on symptornless trees. These experiments show thatD. brevicomis, D. ponderosae, I. paraconfusus, I. latidens, andG. retusus land, apparently indiscriminately, on healthy and stressed hosts. Thus, in these species host discrimination must occur after landing and prior to sustained feeding.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 40
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 7 (1981), S. 359-366 
    ISSN: 1573-1561
    Schlagwort(e): Parasites ; bioassay ; Dendroctonus frontalis ; Coleoptera ; Scolytidae ; olfactometer ; bark beetles
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract An “H”-type olfactometer was designed and built to test the response of bark beetle parasites to various beetle and tree host odors. The design has several advantages over other types of olfactometers. Strong air currents are not utilized, parasites have free movement in the test chamber, and a concentration gradient of test compound is maintained. Parasites tested in the olfactometer demonstrated strong positive responses to air drawn over logs infested with bark beetle larvae and varied responses to tree host odors.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 41
    ISSN: 1573-1561
    Schlagwort(e): Sexual dimorphism ; Tribolium castaneum (Herbst) ; Coleoptera ; Tenebrionidae ; aggregation pheromone ; behavior ; bioassay ; red flour beetle
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Evidence for the existence of a male-produced aggregation pheromone secreted from the prothoracic femoral setiferous sex patch ofTribolium castaneum is reported. Both sexes were attracted toca. 60 ng of crude secretion. Males and females perceive the pheromone on the day of emergence while perception differs between the sexes: male response reaches a maximum on day 1 posteclosion, when tested at 〈1, 1, and 30 days; females show a maximum response at 30 days posteclosion. Behavioral responses to pheromone odors and a complex Chromatographic profile are reported.
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  • 42
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 7 (1981), S. 889-894 
    ISSN: 1573-1561
    Schlagwort(e): α-Pinene ; defensive secretions ; interspecific variation ; limonene ; monoterpenes ; pentadecene ; Artystona sp. ; Coleoptera ; Tenebrionidae ; lichen ; Parmotrema reticulatum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The defensive secretions of four species of the genusArtystona endemic to New Zealand differ from those of other tenebrionids in that they contain α-pinene and limonene, as well as the more characteristic quinones and alkenes. Adults and larvae ofA. obscura, A. erichsoni, A. rugiceps, andArtystona sp. feed on the lichenParmotrema reticulatum (Taylor), but the terpenes are not sequestered from it. The defensive secretions of the four species show some interspecific variation.
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    Standort Signatur Erwartet Verfügbarkeit
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  • 43
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 7 (1981), S. 1135-1147 
    ISSN: 1573-1561
    Schlagwort(e): Ips paraconfusus ; Coleoptera ; Scolytidae ; Pinus ponderosa ; pheromone biosynthesis ; bark beetle ; myrcene ; α-pinene ; ipsenol ; ipsdienol ; cis-verbenol ; myrtenol ; attractants ; pheromones
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Attraction of maleIps paraconfusus to male-infested ponderosa pine logs was inhibited by volatiles from logs infested with mated males and females over an 8-day period in the field. The response of females during this time was not significantly inhibited by these volatiles. Synthesis of the male-specific pheromones, ipsenol and ipsdienol, appeared negligible after 8 days in males allowed to mate with 3 females in these logs while males alone contained levels of these pheromones at about half their maximum rate. The decline in pheromone production in mated males appears to result, at least in part, from a reduction in the activity of the biosynthetic system that converts the host monoterpene, myrcene, to ipsenol and ipsdienol. Mating and feeding have apparently no effect on the biosynthetic system that converts the host monoterpene, (−)-α-pinene, to the pheromone,cis-verbenol in either males or females. The reduced production and release of pheromones by males after mating appears to play a major function in the process of terminating the aggregation phase of host colonization.
    Materialart: Digitale Medien
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  • 44
    ISSN: 1573-1561
    Schlagwort(e): Dihydromatricaria acid ; antifeedant ; acetylenic acid ; Chauliognathus pennsylvanicus ; Coleoptera ; Cantharidae ; soldier beetles ; Phidippus ; jumping spider ; bioassay ; defensive secretion ; defensive behavior
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The acetylenic acid,Z-dihydromatricaria acid (DHMA), previously isolated from the defensive secretion ofChauliognathus lecontei, and now shown to occur also inC. pennsylvanicus, is a potent feeding deterrent to jumping spiders (Phidippus spp.). A simple bioassay withPhidippus is described, which is generally applicable to studies dealing with the isolation and evaluation of feeding deterrency of natural products from insects. By use of this assay,Phidippus were shown to be sensitive to as little as 1 μg DHMA, an amount equivalent to less than 2% of the DHMA content ofC. pennsylvanicus.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 45
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 7 (1981), S. 175-182 
    ISSN: 1573-1561
    Schlagwort(e): Hydrocarbons ; n-alkanes ; branched-chain alkanes ; GC-MS ; cuticular wax ; insect ; cowpea weevil ; Callosobruchus maculatus ; Coleoptera ; Curculionidae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The composition of the cuticular hydrocarbons of the cowpea weevil,Callosobruchus maculatus (F.), was determined by using combined gas chromatography-mass spectrometry. The hydrocarbons constituted 88% of the cuticular lipids and were composed of four homologous series of alkanes. Mono- and dimethyl branched-chain alkanes made up 83% of the hydrocarbon fraction.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 46
    ISSN: 1573-1561
    Schlagwort(e): Pheromone ; Rhyzopertha dominica ; lesser grain borer ; Coleoptera ; Bostrichidae ; unsaturated ester ; dominicalure ; 1-methylbutyl (E)-2-methyl-2-pentenoate ; 1-methylbutyl (E)-2,4-dimethyl-2-pentenoate
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Volatiles from lesser grain borers,Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), were collected on Porapak Q, and those from the male beetles were shown to contain two compounds, that were attractive individually and in combination to both sexes. These compounds were identified as (S)-(+)-1-methylbutyl (E)-2-methyl-2-pentenoate and (S)-(+)-1-methylbutyl (E)-2,4-dimethyl-2-pentenoate by spectrometry and comparison with synthesized compounds. The two compounds have been assigned the trivial names dominicalure 1 and dominicalure 2, respectively. Synthesized samples of these compounds, individually and in combination, were effective in trapping both sexes in field studies.
    Materialart: Digitale Medien
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  • 47
    ISSN: 1573-1561
    Schlagwort(e): electroantennogram ; boll weevil ; grandlure ; pheromone ; Anthonomus grandis Boh. ; Coleoptera ; Curculionidae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Electroantennogram techniques were used to elucidate antennal olfactory response of male and female boll weevils to a dilution series of grandlure, its components, and some vicinal dimethyl analogs. At higher concentrations, response to the mixture of the two aldehyde components of grandlure was significantly higher than to the two alcohol components. Only one vicinal dimethyl analog elicited a significantly higher response than the control. There were no significant differences in response due to sex over all compounds.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 48
    ISSN: 1573-1561
    Schlagwort(e): Tramiathaea cornigera ; Thamiaraea fuscicornis ; Aleocharinae ; Coleoptera ; Staphylinidae ; defensive secretion ; ethyl decanoate ; esters ; undecane ; toluquinone
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The defensive secretions of bothTramiathaea cornigera andThamiaraea fuscicornis contain undecane, toluquinone, ethyl decanoate and smaller amounts of other esters. The presence of esters particularly ethyl decanoate as a major component sets these two beetles apart chemically not only among the staphylinids but also within the subfamily Aleocharinae.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 49
    ISSN: 1573-1561
    Schlagwort(e): Pinus ponderosa ; Dendroctonus brevicomis ; western pine beetle ; attractant ; interruption ; behavior ; pheromone ; Coleoptera ; Scolytidae ; exo-brevicomin ; frontalin ; myrcene ; verbenone
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The catch of the western pine beetle,Dendroctonus brevicomis, at an attractive source of racemicexo-brevicomin, racemic frontalin, and myrcene was reduced by surrounding the source with a grid of 48 stations releasing all three compounds together, orexo-brevicomin alone or myrcene alone. Each compound was released at the rate of 2 mg/24 hr/station. The catch at an attractive bolt cut from a tree being colonized byD. brevicomis was not reduced byexo-brevicomin, but was reduced by the combination ofexo-brevicomin, frontalin, and myrcene in one of two tests. When a transect of traps was placed across a 0.81-hectare plot at six of the 48 stations releasing all three compounds, more beetles were caught at outer than at inner traps. More beetles were caught at unbaited traps on trees in a plot when the three compounds were released than when onlyexo-brevicomin or no compounds were released. A few trees were attacked byD. brevicomis in some of the plots. The antiattractant verbenone released from 48 stations at the rate of 4 mg/24 hr/station did not reduce the catch at an attractive tree bolt.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 50
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 7 (1981), S. 209-220 
    ISSN: 1573-1561
    Schlagwort(e): Ips paraconfusus ; Coleoptera ; Scolytidae ; phloem ; ponderosa pine ; host selection ; feeding stimulants ; extracts ; bark beetle
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract MaleIps paraconfusus Lanier bored and fed in cellulose powder substrates treated with solvent extracts of ponderosa pine phloem in preference to cellulose powder alone. Stimuli that elicit boring and feeding behavior occurred in the water extracts and the combined solvent extracts. No significant boring or feeding occurred in the methanol extract. There was a preference for, but no significant feeding in, the water partition of the ether extract. Feeding, but no preferential boring, occurred in the ether extracts.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 51
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 215 (1981), S. 171-179 
    ISSN: 1432-0878
    Schlagwort(e): Polychaeta, Serpulidae ; Gamete ; Larva ; Development ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Gametes and developing larvae of the polychaete Galeolaria caespitosa were examined by scanning electron microscopy. The sperm display a primitive morphology. When treated with 0.33 M CaCl2, they release a branched acrosomal process. At spawning, the polygonal oocytes have a granular surface made up of spherules and the tips of microvilli. The oocyte coat develops a ridged appearance as the oocyte rounds up. At fertilization, the microvilli are withdrawn from the coat surface. Microvilli again appear on the coat surface during the trochophore stage, but the egg coat appears to be retained as the larval cuticle until the demersal stage. The surface of the larva now shows many microvilli. Details of the organization of several ciliary structures are clarified. Moreover, the present study shows rapid, sequential development of paired setal sacs, with the most anterior pair appearing first.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 52
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 215 (1981), S. 397-415 
    ISSN: 1432-0878
    Schlagwort(e): Stomach ; Epithelium ; Teleost ; Development
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The development of the stomach of the teleost, Clarias lazera, during the early posthatching period, is described, and the developing stomach is compared with that of adult Clarias. The stomach develops in two distinct parts: the corpus, which differentiates first, and the pylorus. The corpus contains a mucous surface epithelium, arranged in folds, and a tubular gland system containing only one type of gland cell, to which the secretion of pepsinogen and HCl is attributed. The pyloric region does not contain tubular glands. From the ultrastructure of the gland cells, the 3H-thymidine labeling index, and the onset of acid production (as determined with pH indicators) it is concluded that a functional stomach is present in juveniles with a standard length of ± 11 mm (approximately 12 days after fertilization at 23–24° C). The ultrastructure of the intestinal epithelium has also been studied. The intestine consists of three segments, similar to those described for stomachless teleosts and a number of fish larvae. In larvae as well as in juveniles, the enterocytes of the second segment show pinocytosis of horseradish peroxidase, although in the juveniles the stomach has already developed. This second segment has the same relative length in all studied larvae and juveniles and is also present in adult Clarias. It is therefore concluded that the capacity to absorb protein macromolecules is not specifically related to the absence of a functional stomach in this teleost species.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 53
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 219 (1981), S. 297-311 
    ISSN: 1432-0878
    Schlagwort(e): Choroid plexus (chick) ; Third ventricle ; Ependyma ; Development ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The surface morphology of the diencephalic choroid plexus (Pl. ch. v. III) was investigated by light microscopy and scanning electron microscopy in chicks from the 7th embryonic day (ED) to the 8th week after hatching. Pl. ch. v. III develops on the anterior ventricular roof from a sagittally oriented fold and a few posteriorly located transverse folds. On the 7th ED no significant differences in the cell surface morphology between Pl. ch. v. III and the surrounding ependyma are observed: both are covered with cilia. During the next four days, long cell prolongations (one per cell) covered with microvilli develop first on the surface of the posterior ventricular roof and then on the posterior part of Pl. ch. v. III. These structures are transitory. On the 11th ED, round cell prolongations (one per cell) appear progressively on the entire plexus, also replacing the long ones. Now the plexus surface is distinct from the surface of the surrounding ependyma. During the last week before hatching and also after hatching, the round cell prolongations become less prominent. Simultaneously, the number of cilia per unit surface area diminishes. With consideration of earlier reports, this study suggests that the following factors are involved in the increase of the surface area of Pl. ch. v. III: (I) The pseudostratified epithelium changes into columnar epithelium. (2) Ependymal elements of the posterior roof of the 3rd ventricle contribute to the anlage of Pl. ch. v. III. In later stages, however, Pl. ch. v. III grows only by mitoses.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 54
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 220 (1981), S. 337-347 
    ISSN: 1432-0878
    Schlagwort(e): Pineal ; Monolayer culture ; Development ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The morphological development of pinealocytes maintained in monolayer culture, without the neural and humoral effects present in the developing rat has been studied and compared with the development that occurs in vivo. Pinealocytes in 5 day cultures contained organelles that were similar to those present in the pineals of intact 5 day old rats. However, light and dark cells were not noted in culture, and the cultured cells did not have the dense granules noted in vivo. As pinealocytes developed in culture, cytoplasmic processes increased in length and number. By 21 days of culture age, synaptic ribbons were found to have decreased in number, the difference between light cell and dark cell cytoplasm had become more prominent, and dense-cored vesicles had become more numerous, just as in the developing gland in vivo. These results suggest that the complex neural and humoral factors impinging upon the developing neonatal pineal in the intact animal may not be necessary for some aspects of its ultrastructural differentiation.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 55
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 214 (1981), S. 271-278 
    ISSN: 1432-0878
    Schlagwort(e): Blood-CSF barrier ; Blood-brain barrier ; Horseradish peroxidase ; Choroid plexus ; Development ; Chick embryo
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The penetration of horseradish peroxidase (HRP) from blood into ventricle via the avian choroid plexus was examined by electron microscopy in order to study the development of the blood-cerebrospinal fluid barrier (BCSFB). 1–20 mg HRP was injected into the allantoic vein of chick embryos between the 7th and 21st day of incubation. Until the 8th day (stage 34) the reaction products of injected HRP were observed in the interepithelial clefts at both luminal (ventricular) and abluminal sides. At the 9th day (stage 35), their penetration was blocked at most apical junctional complexes of the choroidal epithelia. At the 10th day (stage 36) and at every subsequent stage, HRP molecules were completely impeded at the apical tight junctions. These results show that the BCSFB to HRP in the avian choroidal epithelium is completely established by the 10th day, when molecules of HRP are still able to permeate between the capillary endothelia in the cerebellum and the spinal cord (Wakai and Hirokawa 1978).
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 56
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 218 (1981), S. 41-58 
    ISSN: 1432-0878
    Schlagwort(e): Supraependymal cell clusters ; Ventricular system ; Development ; Hypothalamus rat ; Monosodium glutamate ; Androgenized rats
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The histological patterns of supraependymal cell clusters (CC) in rats of different ages (untreated, androgenized, and treated with monosodium glutamate) were investigated with light (LM)-, scanning-and transmission electron microscopy (SEM, TEM). These clusters were a frequent but not a constant finding. In 18 day-and older embryos, CC were always found in the recess of the olfactory bulb immediately prior to its obliteration. All other CC appear in the infundibular recess between the 3rd and the 6th postnatal day. Independent of age, all cell clusters exhibit small aggregates of subependymal tissue protruding through the ependyma. Both neurons (light cells) and neuroglia (dark cells) were found in the CC. By use of SEM, in the region of the infundibular recess it is possible to distinguish four forms of supraependymal cell clusters according to localization, size, number of cells, and presence of intraventricular axons. CC may be 1) receptors or have an additional secretory function; 2) manifestations of a pathological type of reaction of the ventricular wall; 3) possible excrescences of the neural matrix, or 4) modifications of the ventricular wall in relation to the obliteration of the ventricular recesses. The first two interpretations are not tenable based on the present observations.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 57
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 221 (1981), S. 431-442 
    ISSN: 1432-0878
    Schlagwort(e): Kidney (rabbit) ; Development ; Mesonephric nephron ; Freeze-fracture electron microscopy ; Intercellular junctions
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In freeze-fracture replicas of the entire cross-fractured mesonephros of 18 day rabbit embryos the basolateral and luminal cell faces of the different nephron segments were studied and compared with their metanephric counterparts. In the proximal tubule, the shallow zonula occludens exhibited only 1–2 strands and resembled the corresponding metanephric zonula, a very “leaky” type, which was found with a considerable paracellular flow component in sites of isotonic reabsorption. Gap junctions were restricted to the proximal tubule and were seen more frequently in its terminal segment. The distal tubule harboured two types of tight junctions. The most common type, a band of 5–8 closely parallel strands, matched the zonula occludens of the metanephric straight distal tubule. The observed particle density of the basolateral membrane (2,500±328/μm2) was less than that of the proximal tubule (2,642±306). In addition, the collecting tubule exhibited a zonula occludens of the “tight” variety similar to that which occurred in the metanephric collecting duct. Rod-shaped particles of the luminal membrane were mainly concentrated in some of the intercalated cells but also had developed on principal cells, and occasionally, in the distal tubule. The Wolffian duct, with a deep “tight” zonula occludens, had an obviously rather inactive epithelium with no conspicuous transport-linked membrane specializations.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 58
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 220 (1981), S. 251-262 
    ISSN: 1432-0878
    Schlagwort(e): Meiosis ; Spermiogenesis ; Organ culture ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In vitro spermatogenesis of isolated single spermatocyte cysts of Drosophila hydei was studied by microscopic observations and time-lapse cinematography. Cysts of spermatocytes isolated during diplotene develop as far as the coiling stage of spermatid differentiation. The existence of an interphase between meiosis I and meiosis II is, for the first time, documented. Meiosis, Nebenkern formation, and elongation of spermatids occur just as in D. melanogaster; however, an individualization cone, as described for D. melanogaster, can not be detected.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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