Summary
When complementary fragments of an imaginal disc ofDrosophila are cultured for several days prior to metamorphosis, usually one fragment will regenerate while the other will duplicate. It has been proposed that wound healing plays an important part in disc regulation (French et al. 1976; Reinhardt et al. 1977) by initiating cell proliferation and determining the mode of regulation. We tried to delay the wound healing process by leaving a region of dead cells between the wound edges. In “06” fragments (Bryant 1975a) wound healing has occurred after 1–2 days of culture and the regeneration of missing structures after 2–4 days of culture. We observed that leaving a region of dead cells between the wound edges delays both wound healing and the regeneration of missing structures by 2 days.
When disc fragments are cultured in female abdomens and then exposed to3H-thymidine to label replicating cells, then the label is found to be localised around the wound. We observed that delaying wound healing does not delay this localisation of labelled nuclei indicating that wound healing may not be required to initiate DNA replication.
Similar content being viewed by others
References
Adler PV, Bryant PJ (1977) Participation of lethally irradiated imaginal disc tissue in pattern formation inDrosophila. Dev Biol 60: 293–304
Bryant PJ (1975a) Pattern formation in the imaginal wing disc ofDrosophila melanogaster: Fate map, regeneration, and duplication. J Exp Zool 193: 49–78
Bryant PJ (1975b) Regeneration and duplication in imaginal discs. Ciba Found Symp 29: 71–93
Bownes M, Sang JH (1974) Experimental manipulations of earlyDrosophila embryos 1. Adult and embryonic defects resulting from microcautery of nuclear multiplication and blastoderm stages. J Embryol Exp Morphol 32: 253–272
Chan LN, Gehring W (1971) Determination of blastoderm cells inDrosophila melanogaster Proc Natl Acad Sci USA 68: 2217–2221
Dale L, Bownes M (1980) Is regeneration inDrosophila the result of epimorphic regulation? Wilhelm Roux's Archives 189: 91–96
French V, Bryant PJ, Bryant SV (1976) A theory of pattern regulation in epimorphic fields. Science 193: 969–981
Gehring W (1972) In Ursprung H, Nöthiger R (eds) The biology of imaginal discs. Springer, Berlin New York, pp 35–58
Guillermet C, Mandaron P (1980) In vitro imaginal disc development and moulting hormone. J Embryol Exp Morphol 57: 107–118
Haynie JL, Bryant PJ (1976) Intercalary regeneration in imaginal wing disc ofDrosophila melanogaster. Nature 259: 659–662
Haynie JL, Schubiger G (1979) Absence of distal to proximal intercalary regeneration in imaginal wing discs ofDrosophila melanogaster. Dev Biol 68: 157–161
Kumar K, Ouwencel WJ, Faber J (1979) Differentiation capacities of the labial imaginal disc ofDrosophila melanogaster. Wilhelm Roux's Archives 186: 57–64
Lindsley DL, Grell EH (1968) Genetic variations ofDrosophila melanogaster. Carnegie Inst Washington Publ 627
Meer JM van der, Ouweneel WJ (1974) Differentiation capacities of the dorsal metathoracic (haltere) disc ofDrosophila melanogaster. II regeneration and duplication. Wilhelm Roux's Archives 174: 361–373
Murphy C (1972) Localisation of primordia within the dorsal mesothoracic disc ofDrosophila. J Exp Zool 179: 51–62
Nöthiger R (1964) Differenzierungsleistungen in Kombinaten, hergestellts aus Imaginalscheiben verschiedener Arten, Geschlechter und Körpersegmente vonDrosophila. Wilhelm Roux's Archives 155: 269–301
Remhardt CA, Hodgkin NM, Bryant PJ (1977) Wound healing in the imaginal discs ofDrosophila. 1. Scanning electron microscopy of normal and healing wing discs. Dev Biol 60: 238–258
Schubiger G (1971) Regeneration, duplication, and transdetermination in fragments of the leg disc ofDrosophila melanogaster Dev Biol 26: 277–295
Ursprung H (1967) In vivo culture ofDrosophila imaginal discs. In: Wilt FH, Wessels NK (eds) Methods in developmental biology. Crowell, New York
Wigglesworth VB (1937) Wound healing in an insect (Rhodnius prolive Hemiptera). J Exp Biol 14: 364–381
Wilcox M, Smith RJ (1977) Regenerative interactions betweenDrosophila imaginal discs of different types. Dev Biol 60: 287–297
Wright DA, Lawrence PA (1981) Regeneration of segment boundaries inOncopeltus: Cell lineage. Dev Biol
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dale, L., Bownes, M. Wound healing and regeneration in the imaginal wing disc ofDrosophila . Wilhelm Roux' Archiv 190, 185–190 (1981). https://doi.org/10.1007/BF00848301
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00848301