Abstract
In a permanent cell line derived from Drosophila embryos, cytoplasmic actin is produced as an unstable precursor, which is subsequently converted to a stable form. This conversion results in a reduction in isoelectric point, with no apparent change in molecular weight. The conversion involves an enzymatic acetylation, and results in an insensitivity to aminopeptidase digestion, suggesting N-terminal blockage. Both the acetylated and unacetylated actins can participate in the assembly of F-actin, but with different efficiencies.
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This work was supported by a grant from the NIH (GM 22866).
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Berger, E.M., Cox, G., Weber, L. et al. Actin acetylation in Drosophila tissue culture cells. Biochem Genet 19, 321–331 (1981). https://doi.org/10.1007/BF00504277
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DOI: https://doi.org/10.1007/BF00504277