ISSN:
1572-9931
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Hybrid genes containing mRNA encoding sequences for herpes virus thymidine kinase (tk), chloramphenicol acetyltransferase (CAT), orDrosophila alcohol dehydrogenase (Adh), ligated to truncatedDrosophila melanogaster heat-shock protein 70 (hsp 70) gene promoters or to synthetic sequences containing one or several copies of a previously defined heat-shock consensus sequence, were transfected into culturedDrosophila line S3 cells. Each construction was then assayed for gene expression at 25° C and 37° C, using a CAT enzyme assay, slot blot hybridization, or S1 nuclease protection analysis. In theDrosophila cell transient expression assay system, we found that deletions extending beyond position −97, or synthetic constructions containing a single heat shock consensus sequence, were not induced by high-temperature shock. In constructions containing deletions extending to position −186, −130, or −97, in the hsp 70 promoter, and in synthetic constructions containing tandemly spaced heat-shock consensus sequences mRNA transcription was greatly induced by high temperature.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01539914
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