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  • 1
    Electronic Resource
    Electronic Resource
    Sex-Ratio Manipulation in Relation to Insect Pest Control (1983)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Genetics 17 (1983), S. 191-214 
    Details
    ISSN: 0066-4197
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.ge.17.120183.001203
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  • 2
    Electronic Resource
    Electronic Resource
    Existence of an Optimum Expression Level for Secretion of Foreign Proteins in Yeast (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 745 (1994), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1994.tb44385.x
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  • 3
    Electronic Resource
    Electronic Resource
    The genome of the Mediterranean fruitflyceratitis capitata: Localization of molecular markers by in situ hybridization to salivary gland polytene chromosomes (1992)
    Springer
    Chromosoma 101 (1992), S. 448-455 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We hybridized cloned DNA segments to salivary gland polytene chromosomes of the medfly,Ceratitis capitata, and thus established molecular markers for 24 sites on 6 out of 10 autosomal arms. An additional marker identified a medfly repetitive element that hybridizes to approximately 100 autosomal sites as well as a granular network that is thought to represent theX chromosome. Some of the markers correspond to 9 characterized transcription units, while 17 remain anonymous; at least 3 of the latter are restriction fragment length polymorphism (RFLP) markers. The characterized transcription units document that chromosomal arm 5L ofC. capitata is homologous to theDrosophila melanogaster X chromosome, in agreement with previous inferences based on the extensive conservation of linkage groups in Diptera.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00582839
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic sexing in Drosophila melanogaster using the alcohol dehydrogenase locus and a Y-linked translocation (1981)
    Springer
    Theoretical and applied genetics 59 (1981), S. 23-24 
    Details
    ISSN: 1432-2242
    Keywords: Genetic sexing ; Alcohol dehydrogenase ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary By incorporating ethanol (4% v/v) into the larval rearing medium of a specially constructed Drosophila melanogaster strain it was possible to produce only male adults; the female larvae died. In this strain, the male determining chromosome was linked with a positive Alcohol dehydrogenase (ADH) allele by a translocation. The females were homozygous for the null allele and hence sensitive to ethanol. This genetic sexing method is discussed in relation to its use in the genetic control of insects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00275770
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  • 5
    Electronic Resource
    Electronic Resource
    Genetic sexing in the mediterranean fruit fly, Ceratitis capitata, using the alcohol dehydrogenase locus (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 455-457 
    Details
    ISSN: 1432-2242
    Keywords: Genetic sexing ; ADH ; Allyl alcohol ; Ceratitis capitata ; Medfly
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using the alcohol dehydrogenase (ADH) locus a genetic sexing system is being developed in the Mediterranean fruit fly Ceratitis capitata based on the sensitivity of ADH null mutations to environmental ethanol. A series of null mutants have been induced at this locus, however, none proved viable as homozygotes. One of these null mutants was translocated to the male determining chromosome and this line can be used for genetic sexing. When larvae from this line were reared on larval medium containing various concentrations of allyl alcohol, 97% of the emerging adults were males; in the absence of the allyl alcohol the sex ratio in the line is distorted in favour of the females. It is proposed that the higher ADH activity of the females (homozygous positive) in comparison with the males (heterozygous null) is responsible for their lower survival in larval medium containing allyl alcohol. ADH converts the allyl alcohol to the lethal ketone. The possible use of this line to sex large populations of medflies for use in sterile insect release programmes is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289526
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  • 6
    Electronic Resource
    Electronic Resource
    A simple method for the isolation of allelic series using male-linked translocations (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 305-306 
    Details
    ISSN: 1432-2242
    Keywords: Translocations ; Adh ; Genetic sexing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using Adh null alleles a genetic sexing technique is being developed in Ceratitis capitata. In order to facilitate the isolation of a whole series of null alleles at this locus a technique utilizing male-linked translocations is described. It provides a simple efficient and general method for the isolation of any allelic series in species where little genetic information is available.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272865
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  • 7
    Electronic Resource
    Electronic Resource
    Translocations induced by fast neutrons and X-rays in Delia antiqua (1981)
    Springer
    Theoretical and applied genetics 59 (1981), S. 307-312 
    Details
    ISSN: 1432-2242
    Keywords: Translocations ; X-radiation ; Asymmetrical exchanges ; Sib-crossing ; Delia antiqua
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A comparison was made using X-rays and fast neutrons for the induction of translocations in Delia antiqua. Using the same radiation dose, no difference in efficiency between the two radiation types could be observed. However, with fast neutrons many multiple translocations were induced, including a quadruple translocation involving 4 out of 5 autosomes. One male linked translocation was also induced. The reciprocal translocations were assigned into two classes: symmetrical and asymmetrical, and ten of the latter were chosen for inbreeding to produce homozygotes. Asymmetrical exchanges were chosen so that translocation homozygotes could be differentiated cytologically from the normal karyotype. In seven different translocations, homozygous larvae were observed, but often at a low frequency. In four of these lines, viable adult homozygotes were observed. Subsequent random sib-crossing failed to produce a homozygous line.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264983
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  • 8
    Electronic Resource
    Electronic Resource
    Controlled crosses and cage experiments with a translocation in Drosophila (1973)
    Springer
    Genetica 44 (1973), S. 591-601 
    Details
    ISSN: 1573-6857
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Predictions for the use of autosomal translocations for pest control have been made on the assumptions that translocation heterozygotes are semi-sterile and translocation homozygotes are fully fertile and have viabilities which are either equal to or slightly less than that of the wild type. These parameters were assessed in controlled crosses using a translocation in Drosophila and they showed good agreement with expectation. It was expected that in a mixed population of such translocations and wild types, the karyotype at the higher frequency would be favoured by selection. Two cage experiments were initiated with mixed populations of translocation homozygotes and wild types at a frequency of 9:1 in favour of the translocation. Contrary to expectation, the translocation was eliminated from both populations. During this process, there was a reduction in population fertility. Computer studies showed that the results were consistent with a reduction in fitness of the translocation homozygote to about 0.5, i.e. about the same as the semi-sterile heterozygote, so that negative heterosis did not exist and hence frequency dependent selection could not occur.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00116813
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  • 9
    Electronic Resource
    Electronic Resource
    Ceratitis capitata—a suitable case for genetic sexing (1982)
    Springer
    Genetica 58 (1982), S. 229-237 
    Details
    ISSN: 1573-6857
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the framework of the development of a genetic sexing mechanism male-linked translocations were induced and studied in the Mediterranean fruit fly, Ceratitis capitata. Two-day old males were irradiated with 5 krad of X-rays and mated to virgin females. The induced translocations were selected by identifying linkage between a visible marker (eye colour or pupal colour) and sex. A total of 18 translocations were isolated in three irradiation series. All the lines except one showed complete linkage of the sex with the marker. In the one exception a small percentage of ‘recombinant males’ was consistently found but they proved to be sterile. Very few recombinant females were found. The origin of these males is at present unclear. Of the six lines which were tested all showed significantly reduced larval survival and 4 had reduced pupal survival. The egg fertility of the lines ranged from 88.39% down to 25.39%. The third irradiation series was used to check if families showing intermediate linkage were present; this could infer that crossing-over was occurring in the male. However, only two families out of 148 tested showed such linkage. It was concluded that crossing-over in the male medfly will not be a serious obstacle to the development of a genetic sexing method using linkage of resistant conditional lethal alleles to the male-determining chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00128017
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  • 10
    Electronic Resource
    Electronic Resource
    Unexpected segregation ratios from male-linked translocations in the Mediterranean fruitfly, Ceratitis capitata (Diptera: Tephritidae) (1984)
    Springer
    Genetica 62 (1984), S. 209-215 
    Details
    ISSN: 1573-6857
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three male-linked translocations were isolated in the Mediterranean fruitfly, Ceratitis capitata (Wiedemann) which showed sex linkage for an autosomal pupal colour marker, white pupa (wp). The wild type allele (wp −) was translocated to the male-determining chromosome. Males emerge from wild type pupae (brown) and females from white pupac. These lines will be used to develop an automatic sexing system. During maintenance of these lines in large populations it was noted that in two of them there was a significant distortion in the expected 1: 1 segregation ratio for pupal colour and excess white pupae were produced. However, adults did not emerge from a large proportion of these pupae. Using known numbers of 1st instar larvae from the three lines, the distortion was further studied and the effect confirmed. To account for this pupal colour segregation distortion it was assumed that certain duplication/deficiency zygotes survive to the late pupal stage. These zygotes have a deficiency for the wild type allele, therefore the normally recessive mutant allele wp could be expressed (‘pseudo-dominance’). The overall fitness of the three lines was calculated, and their suitability for the genetic sexing of large populations is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00056438
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