Skip to main content
Log in

Normal and lectin-mediated aggregation in aDrosophila cell line

  • Rapid Communication
  • Published:
Wilhelm Roux's archives of developmental biology Aims and scope Submit manuscript

Summary

In this paper we describe the aggregation of cells from embryo-derived cell lines ofDrosophila, measured by examining the ability of single cells to adhere to one another when suspended in culture medium and swirled on a rotary shaker. Using this method we demonstrated the presence of receptors for Concanavalin A, soybean agglutinin, and possibly wheat germ agglutinin on the surface of Schneider's line-2 cells. Our work provides basic descriptive and background information for further studies onDrosophila cells, including those isolated from imaginal discs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Andrews CA (1975) Surface properties and carbohydrate metabolism of the cells ofDrosophila melanogaster grown in vitro. PhD Thesis, University of Michigan

  • Andrews CA, Rizki TM (1978) Studies on Lectin-induced agglutination ofDrosophila embryonic cell lines. J Insect Physiol 24:9–12

    Google Scholar 

  • Barbera AJ, Marchase RB, Roth S (1973) Adhesive recognition and retinotectal specificity. Proc Natl Acad Sci 70:2482–2486

    Google Scholar 

  • Becker J (1972) Fusions in vitro de cellules somatiques en culture deDrosophila melanogaster, induites par la Concanavaline A CR Acad Sci Paris 275:2969–2972

    Google Scholar 

  • Bryant P (1979) Pattern formation in imaginal discs. In: Ashburner M, Wright TRF (eds). The Genetics and biology ofDrosophila; vol 2C. Academic Press, New York, pp 230–336

    Google Scholar 

  • Fox AS, Horikawa M, Ling LL (1968) The use ofDrosophila cell cultures in studies of differentiation. In Vitro 3:65–84

    Google Scholar 

  • Garcia-Bellido A, Lewis EB (1976) Autonomous cellular differentiation of homeotic bithoray mutants ofDrosophila melanogaster. Dev Biol 48:400–410

    Google Scholar 

  • Garcia-Bellido A, Nöthiger R (1976) Maintenance of determination by cells of imaginal discs ofDrosophila after dissociation and culture in vivo. Wilhelm Roux's Archives 180:189–206

    Google Scholar 

  • Halfer C, Petrella L (1976) Cell fusion induced by Lysolecithin and Concanavalin A inDrosophila melanogaster somatic cells cultured in vitro. Exp Cell Res 100:399–404

    Google Scholar 

  • Holtfreter J, (1939) Tissue affinity, a means of embryonic morphogenesis. Reprinted in translation in: Willier BA, Oppenheimer JM (eds) Foundations of experimental embryology. Prentice Hall, New Jersey, original paper

    Google Scholar 

  • Ling LL, Horikawa M, Fox A (1970) Aggregation of dissociated cells fromDrosophila embryos. Dev Biol 22:264–281

    Google Scholar 

  • Moscona A (1961) Rotation-mediated histogenetic aggregation of dissociated cells. Exp Cell Res 22:455–475

    Google Scholar 

  • Nicholson G (1974), The interactions of Lectins with animal cell surfaces. Int Rev Cytol 39:90–190

    Google Scholar 

  • Rizki R, Rizki T, Andrews C (1977) Modification ofDrosophila cell surfaces by Concanavalin A. Cell Tiss Res 185:183–190

    Google Scholar 

  • Rutishauser U, Thiery J, Brackenbury R, Sela B, Edelman G: Mechanisms of adhesion among cells from neural tissues of the chick embryo. Proc Natl Acad Sci 73:577–581

  • Schneider I (1972) Cell lines derived from late embryonic stages ofDrosophila melanogaster. J Embryol Exp Morphol 27:353–365

    Google Scholar 

  • Steinberg M (1964) The problem of adhesive selectivity in cellular interactions. In: Locke M (ed) Cellular membranes in development. 22nd Symposium of the Society for Developmental Biology, pp 321–366.

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Muckenthaler, F.A., Fausto-Sterling, A. Normal and lectin-mediated aggregation in aDrosophila cell line. Wilhelm Roux' Archiv 190, 118–122 (1981). https://doi.org/10.1007/BF00848405

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00848405

Key words

Navigation