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  • Excitons and related phenomena (including electron-hole drops)  (54)
  • Zea mays  (50)
  • Springer  (104)
  • American Meteorological Society
  • Cambridge University Press
  • Cell Press
  • 1995-1999  (104)
  • 1990-1994
  • 1955-1959
  • 1995  (104)
  • 1966
  • 1959
  • 1958
  • 1955
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Keywords
Publisher
  • Springer  (104)
  • American Meteorological Society
  • Cambridge University Press
  • Cell Press
Years
  • 1995-1999  (104)
  • 1990-1994
  • 1955-1959
Year
  • 1
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    Entomologia experimentalis et applicata 77 (1995), S. 315-321 
    ISSN: 1570-7458
    Keywords: Insecta ; Helicoverpa zea ; Zea mays ; resistance inheritance ; joint scaling test ; additive-dominance model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The corn earworm,Helicoverpa zea (Boddie), is a perennial economic pest of field crops in the United States. Maize,Zea mays L., is the major host crop promoting the build-up of devastating corn earworm populations that limit full production of cotton, soybean, peanut, and grain sorghum. Resistance to the corn earworm in maize and in particular sweet maize, would provide an environmentally safe, economical method of control for this pest insect. Antibiotic effects of corn silks on this insect are: small larvae, extended developmental period, and reduced fecundity. Silks from individual maize plants of resistant and susceptible lines and progeny in six generations consisting of parents (P1, P2), F1, F2, and backcrosses BC1.1 (F1 × P1) and BC1.2 (F1 × P2) from each of four crosses were used to determine the genetic basis of the antibiotic resistance of silks to the corn earworm. In the cross of Zapalote Chico × PI340856, genes controlling resistance in the silks to the corn earworm larvae are dominant in PI340856 to those in Zapalote Chico. The cross of Zapalote Chico × GT114 involves parents differing in degree of resistance, and possibly differing for the genetic mechanism by which the resistance is inherited. The inheritance of resistance may involve non-additive (dominance and epistasis) genetic variance. A digenic 6-parameter model indicated (1) the resistance in this cross is controlled by more than one pair of genes and (2) some or all of the genes interact to cause non-allelic interaction. Thus, the resistance in this cross may be controlled by both dominant and recessive genes. The resistance of Zapalote Chico × CI64, an intermediate inbred, is influenced by additive gene effects. The digenic model adequately predicts all generation means of the cross of GT3 × PI340856 except for the F1. Thus, it appears that the additive-dominance model is not satisfactory for this cross involving susceptible and resistant parents. Generation mean analysis indicates that resistance to silk-feeding by corn earworm larvae is under genetic control, but gene action differs from one type of cross to another.
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  • 2
    ISSN: 1432-1890
    Keywords: Glomus mosseae ; Zea mays ; Mineral uptake ; Root exudation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Maize (Zea mays L. cv. Alize) plants were grown in a calcareous soil in pots divided by 30-μm nylon nets into three compartments, the central one for root growth and the outer ones for hyphal growth. Sterle soil was inoculated with either (1) rhizosphere microorganisms other than vesicular-arbuscular mycorrhizal (VAM) fungi, (2) rhizosphere microorganisms together with a VAM fungus [Glomus mosseae (Nicol. and Gerd.) Gerdemann and Trappel], or (3) with a gamma-irradiated inoculum as control. Plants were grown under controlled-climate conditions and harvested after 3 or 6 weeks. VAM plants had higher shoot∶root ratios than non-VAM plants. After 6 weeks, the concentrations of P, Zn and Cu in roots and shoots had significantly increased with VAM colonization, whereas Mn concentrations had significantly decreased. Root exudates were collected on agar sheets placed on the interface between root and hyphal compartments. Six-week-old VAM and non-VAM plants had similar root exudate compositions of 72–73% reducing sugars, 17–18% phenolics, 7% organic acids and 3% amino acids. In another experiment in which root exudates were collected on agar sheets with or without antibiotics, the amounts of amino acids and carbohydrates recovered were similar in VAM and non-VAM plants. However, threeto sixfold higher amounts of carbohydrates, amino acids and phenolics were recovered when antibiotics were added to the agar sheets. Thus, the high microbial activity in the rhizosphere and on the rhizoplane limits the exudates recovered from roots.
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  • 3
    ISSN: 1432-1890
    Keywords: Key words Glomus mosseae ; Zea mays ; Mineral uptake ; Root exudation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Maize (Zea mays L. cv. Alize) plants were grown in a calcareous soil in pots divided by 30-μm nylon nets into three compartments, the central one for root growth and the outer ones for hyphal growth. Sterile soil was inoculated with either (1) rhizosphere microorganisms other than vesicular-arbuscular mycorrhizal (VAM) fungi, (2) rhizosphere microorganisms together with a VAM fungus [Glomus mosseae (Nicol. and Gerd.) Gerdemann and Trappel], or (3) with a gamma-irradiated inoculum as control. Plants were grown under controlled-climate conditions and harvested after 3 or 6 weeks. VAM plants had higher shoot : root ratios than non-VAM plants. After 6 weeks, the concentrations of P, Zn and Cu in roots and shoots had significantly increased with VAM colonization, whereas Mn concentrations had significantly decreased. Root exudates were collected on agar sheets placed on the interface between root and hyphal compartments. Six-week-old VAM and non-VAM plants had similar root exudate compositions of 72–73% reducing sugars, 17–18% phenolics, 7% organic acids and 3% amino acids. In another experiment in which root exudates were collected on agar sheets with or without antibiotics, the amounts of amino acids and carbohydrates recovered were similar in VAM and non-VAM plants. However, three- to sixfold higher amounts of carbohydrates, amino acids and phenolics were recovered when antibiotics were added to the agar sheets. Thus, the high microbial activity in the rhizosphere and on the rhizoplane limits the exudates recovered from roots.
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  • 4
    ISSN: 1432-0789
    Keywords: Arbuscular mycorrhiza ; Limed silty loam Heavy metals ; Pb-Zn smelter ; Root colonization Spore numbers ; Tolerance ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The bioavailability of heavy metals (Cd, Zn, Pb, Cu) and the abundance of arbuscular mycorrhiza (AM) were studied in two agricultural fields close to a Pb-Zn smelter and three fields outside the pollution zone all cultivated with maize (Zea mays L.). Metal extractability with ethylenediaminetetraacetic acid (EDTA)-NH4OAc and Ca(NO3)2, plant metal uptake, and mycorrhizal parameters (spore number, root colonization) were assessed at two growth stages (six-leaf and maturity). Despite regular liming, the availability of Cd, Zn, and Pb was markedly higher in the two metal-polluted fields than in the three uncontaminated fields. However, the AM abundance was not correlated with metal availability. Root colonization and spore numbers in the metal polluted fields were relatively high, though at plant maturity the former was significantly lower than in one of the uncontaminated fields. The very low AM abundance in the two other unpolluted fields was related to other factors, particular soil and plant P status and soil pH. AM root colonization did not substantially prevent plant metal accumulation, since the metal concentrations in maize grown on the polluted fields strongly exceeded normal values, and for Cd and Pb reached the limits of toxicity for animal feed.
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  • 5
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    Biology and fertility of soils 20 (1995), S. 57-62 
    ISSN: 1432-0789
    Keywords: Nitrogen use ; Nitrogen fertilizer recovery ; Zea mays ; Phaseolus vulgaris ; Vigna unguiculata ; Nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Locally suitable cultivars of maize, beans, and cowpeas were grown in field experiments for four seasons in semi-arid Kenya. For three seasons, the dry matter production and grain yield of maize and beans were not increased by N fertilizer additions up to 120 kg N ha-1. Fertilizer recoveries measured by 15N isotope dilution techniques were low, less than 20%. Inoculated and uninoculated beans failed to fix N2. By contrast the cowpea derived 50% of its N from fixation, equivalent to 197 kg N ha-1. The N content of the grain generally exceeded 40 kg N ha-1, and the N content of the seeds from the grain legumes were greater than those from the cereals. Large inputs of N fertilizer or N by fixation are required if maize-grain legume cropping system in semiarid Kenya are to be sustained in the long term.
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  • 6
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    Mycopathologia 129 (1995), S. 117-125 
    ISSN: 1573-0832
    Keywords: Biological control ; Corn seedling disease ; Enterobacter cloacae ; Fusarium moniliforme ; Maize ; Seedling blight ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The bacteriumEnterobacter cloacae is presently used for biocontrol of postharvest diseases of fruits and vegetables and as a preplant seed treatment for suppression of damping-off. This bacterium has apparent affinities for several grass species, but it is not considered to be an endophyte. While screening corn for fungi and bacteria with potential for biocontrol of various corn diseases, the surface-sterilized kernels of one unknown Italian corn cultivar produced fungus-free corn seedlings with roots endophytically infected byE. cloacae. This paper describes the microscopic nature ofE. cloacae RRC 101 with corn, and the in vitro control ofFusarium moniliforme and other fungi with this bacterium. Light and electron microscopy determined that this isolate ofE. cloacae was biologically associated with corn seedling roots, where it was distributed intercellularly within the cortex and stele. This is a first report of a strain of this bacterium as an endophytic symbiont of roots. Following a topical application ofE. cloacae to kernels, and upon germination this bacterium readily infected roots of two other corn cultivars. The bacterium was observed within the endosperm of germinating corn seedling, but germination was not affected. Further, the bacterium was isolated from leaves and stems of 3- to 6-week-old seedlings indicating that the above ground portions of corn were also colonized. There was no evidence of damage to cells of the root during a three to four week observation period. This bacterium was antagonistic to several isolates of the corn pathogenFusarium moniliforme, and to two other species of fungi, all of which produce mycotoxins on corn.
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  • 7
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    Mycopathologia 132 (1995), S. 173-183 
    ISSN: 1573-0832
    Keywords: Deoxynivalenol ; Embryo ; Mature ; Ochratoxin ; Plantlet ; Zearalenone ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Mature maize (Zea mays) embryos were exposed to 5, 10 and 25 µg ml−1 of deoxynivalenol (DON), zearalenone (ZEA), ochratoxin A (OA) and a mixture of zearalenone and deoxynivalenol (ZEA/DON) for 9 days. DON and the ZEA/DON combination were consistently more inhibitory of the measured parameters than either ZEA or OA. Based on the predicted additive values, it would appear that, in combination, ZEA and DON act synergistically to inhibit root and shoot growth. For ZEA alone, a concentration of 5 µg ml−1 ZEA was generally inhibitory of root and shoot elongation and fresh mass accumulation, while at 10 and 25 µg ml−1, this toxin had a stimulatory effect on these parameters. For OA, the measured effects on root and shoot growth at 5 and 25 µg ml−1 were stimulatory, while at 10 µg ml−1 OA, an inhibitory effect was observed. For all toxins, inhibitory/stimulatory effects were generally more marked for root parameters than for shoot elongation or mass.
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  • 8
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1315-1322 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Time resolved optical spectroscopies and other ultrafast optical measurements in condensed matter ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary The concept of coherence due to optical excitation of an ensemble of two-level atoms is relatively simple and well established. For the laser-excited electron-hole pairs or excitions in a semiconductor, the Pauli exclusion and the Coulomb interaction lead to the necessary evolution of the theory of coherence to incorporate the spin degree of freedom, the filling of phase space, the excitons and particularly, their correlation. We have developed two theoretical approaches: one using the Feynman diagrams and one in terms of a force-force correlation function. The former gives us a pictorial way to describe the optical processes and the latter gives us a prescription for computation beyond the perturbative approach. In this paper, we will attempt to use simple physical pictures based on our theory to describe the time development of the coherence, the role of correlation, the dependence on the polarization of light, and the nonlinear time-resolved optical experiments which measure the coherence.
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  • 9
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1429-1433 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Localised single-particle electronic states (excluding impurities) ; Photoluminescence ; II–VI semiconductors ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary Time-resolved photoluminescence experiments in a wide carrier density range up to and beyond the threshold for stimulated emission in ZnSe/ZnS x Se1−x superlattices are presented. Different localisation mechanisms of free excitons are identified giving rise to different radiative recombination channels. At high carrier density localised energy states merge into miniband that allows for exciton-exciton interaction, such as screening of the free-exciton absorption and inelastic exciton-exciton scattering.
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  • 10
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1407-1412 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Surface and interface electron states ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary We have investigated the fine structure of luminecence of CdSe nanocrystals observed with size-selective excitation. We show that the luminescence line closest to the laser arises from the recombination of the optically forbidden A-exciton. Radiative recombination occurs via a phonon-assisted virtual transition to theB-exciton state. The electron-hole exchange energy obtained from the experimental results strongly increases with decreasing nanocrystal size as expected from the increasing overlap of the electron and hole wave functions. The size dependence is in good agreement with the theoretical predictions.
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  • 11
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1423-1427 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Surface and interface electron states ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary Normalized reflection spectra in GaInAs/GaAs quantum wells are shown for two sets of samples with different alloy concentration (x=9% and 18.5%) and well thickness ranging from 1.5 nm to 25 nm. All samples were grown on (001) GaAs surface by Molecular Beam Epitaxy and characterized by RHEED and X-ray reflection diffraction. Exciton envelope function in effective mass approximation and optical response in polaritonic schema are computed. Normalized reflection spectroscopy has shown itself to be a well suited technique in order to study structural and electronic properties of confined quantum structures.
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  • 12
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1447-1452 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Optical properties of thin films, surfaces, and layer structures (superlattices, heterojunctions, and multilayers) ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary We investigate the absorption spectrum and the distribution of radiative decay times for two-dimensional excitons in semiconductor quantum wells, as affected by an adiabatic white-noise random potential. Such a potential could result from atomic-scale fluctuations in the composition of an alloy semiconductor, or in quantum well thickness. We find in general that the shortest radiative decay time is directly proportional to the inhomogeneous width of the exciton line in absorption, itself proportional to the correlation parameter for the random potential.
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  • 13
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1473-1479 
    ISSN: 0392-6737
    Keywords: Surface and interface electron states ; Excitons and related phenomena (including electron-hole drops) ; Polaritons (including photon-phonon and photon-magnon interactions) ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary We report the evidence of exciton-polariton centre-of-mass quantization in II–VI quantum wells consisting of ternary alloy. Absorption and photoluminescence studies on Zn0.89Cd0.11Se/ZnSe quantum wells, covering the intermediate region between two- and three-dimensional exciton behaviour, exhibit distinct optical resonances in the high-energy tail of the free-exciton structure. We reproduce the well width dependence of the centre-of-mass states by modeling the optical response of the QWs with a thin-slab variational wave function, which takes into account non-adiabatic terms.
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  • 14
    ISSN: 0392-6737
    Keywords: Studies of specific magnetic materials ; Optical properties of thin films surfaces and layer structures (superlattices, heterojunctions and multilayers) ; Excitons and related phenomena (including electron-hole drops) ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary Two qualitatively different e-h systems have been investigated in undoped CdTe/Cd0.88Mn0.12Te and Cd0.97Mn0.03 Te/Cd0.75Mn0.25 Te QWs at 2 K and magnetic fields up to 14 T, namely a strongly spin-depolarized system in the CdTe QW and a highly spin-polarized one in the Cd0.97Mn0.03 Te QW. The interaction of the spin-aligned excitons is repulsive. In contrast, in the spectra of a dense spin-depolarized excitonic system an additional well pronounced emission lineM was observed at approximately 5 meV below the excitonic line. This line is assigned to the emission of molecules consisting of excitons with opposite spins of both electrons and holes.
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  • 15
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1573-1578 
    ISSN: 0392-6737
    Keywords: Time-resolved optical spectroscopies and other ultrafast optical measurements in condensed matter ; Excitons and related phenomena (including electron-hole drops) ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary We report on the observation of an electric-field-induced electric-dipole moment of biexcitonic molecules in a GaAs/Al0.3Ga0.7As superlattice. The macroscopic oscillating electric field, associated with the microscopic wave packet dipole moments, is monitored by time-resolved transmittive electro-optic sampling. The field dependence of the biexcitonic binding energy is detected by transient four-wave mixing. From these experiments, an ultrafast non-resonant excitation mechanism of biexcitons is inferred.
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  • 16
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1255-1264 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Polaritons (including photon-photon and photon-magnon interactions) ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary We develop the bipolariton concept for a quasi-2D excitonic molecule (m) in quantum wells (QWLs). The bipolariton wave equation, which includes both the exciton-exciton (x-x) attraction and thex-photon (γ) coupling, is analysed. In the 2D bipolariton model, a quasi-2Dm exists in QWLs mainly as two quasi-boundsurface polaritons and, consequently, decays mainly into two outgoing surface polaritons. In quasi-2D GaAs QWLs the polariton renormalizations give rise to a «Mexican hat» structure in them dispersion at small momenta together with a considerable increase of both them effective binding energy ɛ m and the inversem radiative lifetimeГ m . A van Hove singularity in the joint density of polariton states is responsible for this effect.
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  • 17
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1657-1662 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Polaritons (including photon-phonon and photon-magnon interactions) ; Dynamics of nonlinear optical systems ; optical instabilities, optical chaos, and optical spatio-temporal dynamics ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary The linewidth of the microcavity exciton polariton is studied by onresonant and off-resonant photoluminescences. The two main contributions to the linewidth are caused by radiative loss and phonon scattering. The oscillator strength is found to be unmodified by the exciton-phonon coupling. Comparison is made between theory and experiment.
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  • 18
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1717-1722 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Time-resolved optical spectroscopies and other ultrafast optical measurements in condensed matter ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary The coupled dynamics of free carriers and excitons after non-resonant excitation with short laser pulses is investigated by means of an Ensemble Monte Carlo method which includes phonon-assisted formation and dissociation of free excitons. Simulations for bulk GaAs reveal that excitonic binding under participation of LO phonons is a very fast and effective process. The temporal rise of exciton luminescence depends on the particular excitation conditions and is determined by all interaction mechanisms in the system.
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  • 19
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1747-1751 
    ISSN: 0392-6737
    Keywords: Polaritons (including photon-phonon and photon-magnon interactions) ; Excitons and related phenomena (including electron-hole drops) ; Quantum optics ; Electron states in low-dimensional structures (including quantum wells, superlattices, layer structures, and intercalation compounds) ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary A great interest in the study of exciton-photon coupling in resonant optical media such as semiconductor microcavities is currently developing both in experiments and in theory. We investigate the spectral response of a new semiconductor quantum system in which the cavity material itself is the active medium. If the Fabry-Perot quasi-mode is carefully tuned on the excitonic transition of the cavity bulk material, strong exciton-photon coupling takes place and produces new features such as a Rabi-like splitting as large as that observed in quantum well microcavities and comparable optical absorption. In addition, the polaritonic spatial dispersion and the quantization of the centre-of-mass motion introduce remarkable fine structures which are absent in the quantum well case. We demonstrate the above effects from a spectroscopic analysis of GaAs cavities, using standard reflectance and photoluminescence techniques. The experimental results are further clarified with theoretical calculations performed with an adapted transfer-matrix approach. The case of semi-cavities is finally considered and a new way to control the exciton-photon interaction in our system is achieved tailoring the photonic wave function with a change in the reflectivity phase of the mirrors.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1801-1805 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Time-resolved optical spectroscopies and other ultrafast optical measurements in condensed matter ; Electron states in low-dimensional structures (including quantum wells ; superlattices ; layer structures ; and intercalation compounds ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary Disorder-induced Rayleigh scattering of excitons in quantum wells is investigated theoretically. For classical excitions (long-range spatial correlation), the disorder average can be performed exactly. For the full problem, a kinetic equation with «weak memory» for the excition distribution is derived which coincides with the classical result in the appropriate limit. The theoretical results are compared with recent experimental data on femtosecond time-resolved luminescence explaining well the finite rise time and the heavy-light-hole beating.
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  • 21
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1323-1332 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Polaritons (including photon-phonon and photon-magnon interactions) ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary We present an extensive set of absorption and PL measurements on semiconductor microcavities in the strong-coupling regime. We observe strong coupling from 110 K to room temperature. We look at the behaviour of the photoluminescence for different exciton-photon detuning. We discuss this data using two different pictures, the first is a non-interacting (uncoupled) picture of an exciton in a cavity filter. The second model is that of a cavity polariton and this explains the observed dynamics in the strong-coupling regime. We show how the opposing needs of strong coupling and high photoluminescence extraction efficiency can be reconciled.
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  • 22
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1343-1347 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Polarons and electron-phonon interactions ; Electron states in low-dimensional structures (including quantum wells, superlattices, layer structures, and intercalation compounds) ; III-V compounds and systems ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary We have calculated the binding energies of excitons in quantum well structures based on ionic semiconductors by including the electron-hole interactions with the longitudinal-optical-phonon field. We have taken into account these interactions by using different effective interaction potentials between the electron and the hole as derived by Haken, Aldrich and Bajaj, and Pollman and Buttner. We have calculated the binding energies of excitons in several ionic quantum well structures as functions of well width using these effective potentials following a variational approach. We find that the values of the exciton binding energies calculated using these potentials are always larger than those, obtained using a Coloumb potential screened by static dielectric constant.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1509-1512 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; III-V Compounds and systems ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary The reflectance spectra of GaN/6H-SiC, films and the absorption spectra of GaN/Al2O3 films were studied and several fundumental parameters of GaN-energy positions of exciton, resonances ω0, damping constants⩾s, longitudinal-transverse splittings ωLT and the oscillator, strengthsf of the exciton states were determined. A blue shift and broadening of the luminescence band were observed in the luminescence spectra of GaN quantum dots. The mean dot radius in the sample was estimated to be about 4.5nm.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1531-1536 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Time-resolved optical spectroscopies and other ultrafast optical measurements in condensed matter ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary The localization dynamics of excitons within growth islands of GaAs/Al x Ga1−x As single quantum wells (SQW) have been investigated by time-resolved photoluminescence spectroscopy (TRPL). Several samples with different substrate misorientation and growth interruption times are compared with regard to the photoluminescence (PL) emission dynamics. For monolayer (ML) islands larger than the exciton radius,i.e. long growth interruption, the time evolution of the different PL peaks reflects the transfer of free excitons between growth islands as well as the localization within a single growth island. The samples with shorter growth interruption reveal a much less pronounced PL splitting due to ML islands. The dynamics appears to be mainly determined by exciton localization to thicker ML islands. The density of localization centres is larger for the sample grown on misoriented substrates.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1555-1559 
    ISSN: 0392-6737
    Keywords: Nonlinear optics ; Optical properties of thin films, surfaces, and thin layer structures (including superlattices, heterostructures, and intercalation compounds) ; Electron states in low-dimensional structures (including quantum wells, superlattices, layer structures, and intercalation compounds) ; Excitons and related phenomena (including electron-hole drops) ; Conference proceedings
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    Notes: Summary In a system comprising an organic layer coupled to a semiconductor layer, if the energy of the Frenkel exciton in the first is close to that of the Wannier exciton in the second, the lowest electronic excitations can be described as hybrid resonances. These new excitons give rise to very pronounced optical non-linearities because they have at the same time large oscillator strengths, typical of Frenkel excitons, and large radii, typical of Wannier excitons. The results of a microscopic calculation of the optical properties of such a system are presented and discussed: compared with the usual semiconductor quantum wells, enhancements of two orders of magnitude both in the size of the susceptibility (linear part) and in its relative change with increasing excitation density (Kerr non-linearity) are predicted.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1383-1387 
    ISSN: 0392-6737
    Keywords: III–V semiconductors ; Electron states in low-dimensional structures (including quantum wells, superlattices, layer structures, and intercalation compounds) ; Excitons and related phenomena (including electron-hole drops) ; III–V compounds and systems ; Conference proceedings
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    Topics: Physics
    Notes: Summary We report an experimental study on a large set of InGaAs/GaAs quantum well structures by means of continuous-wave photoluminescence and photoluminescence excitation. The luminescence spectrum at low temperature systematically exhibits a doublet structure whose lineshape is very sensitive to the excitation energy. Accordingly, the excitation spectra detected by monitoring the emission at the two different luminescence peaks have very different profiles, with peaks and/or dips which are not directly related to absorption resonances. As a matter of fact, the anomalies disappear when increasing the temperature or when using an optical bias with energy above the GaAs energy gap. In these cases, the excitation profiles recover the behaviour typical of 2D systems.
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    Keywords: Excitons and related phenomena (including electron-hole drops) ; Optical properties of thin films surfaces and layer structures (superlattices, heterojunctions and multilayers) ; Conference proceedings
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    Topics: Physics
    Notes: Summary We report here on the study of the optical properties of ZnCdSe−ZnSe quantum structures elaborated by metalorganic chemical vapour deposition (MOCVD). The band structure is experimentally investigated by means of photoluminescence and photoreflectance. We have computed the exciton binding energies for heavy- and light-hole excitons in the context of a self-consistent two-parameter trial function. As a complement, we study the temperature dependence of the photoluminescence intensity under both direct and indirect photoexcitation in graded-index separate confinement heterostructures based on these materials.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1787-1790 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; II–VI compounds and other chalcogenides ; II–VI semiconductors ; Conference proceedings
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    Notes: Summary We report on the observation of the doubly resonant secondary emission involving 2s and 1s exciton states. The resonance conditions have been realized in CdTe/Cd1-xMnx Te quantum well structures by magnetic-field tuning of the 2s-1s energy spacing to the LO-phonon energy. Theoretical analysis shows the doubly resonant process to be indirect, with hot 1s excitons acting as intermediate states. It is concluded that the resonance width of 2.6 meV gives the 2s-exciton homogeneous linewidth governed by elastic scattering into the hot 1s exciton states.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1277-1284 
    ISSN: 0392-6737
    Keywords: II–IV semiconductors ; Excitons and related phenomena (including electron-hole drops) ; Time-resolved optical spectroscopies and other ultrafast optical measurements in condensed matter ; Conference proceedings
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    Topics: Physics
    Notes: Summary Steady-state and picosecond photoluminescence experiments on CdTe/CdZnTe and CdTe/CdMnTe asymmetric double quantum wells are presented. An external magnetic field is used to tune the coupling between the two QWs by allowing resonances to occur between excitonic states. Very efficient tunnelling is found when an exciton from the high-energy QW can transfer either with the emission of an LO phonon or via the resonance with the 2s state of the low-energy QW.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1339-1342 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Time resolved optical spectroscopies and other ultrafast optical measurements in condensed matter ; Conference proceedings
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    Notes: Summary Fast initial decays of both the luminescence intensity and the circular luminescence polarization at high exciton densities, under resonant excitation, are reported for the first time. These fast decays are simultaneously initiated by the increase of the ellipticity of the photogenerating picosecond laser beam. The theory of the mechanism illustrates the driving role of the interexciton exchange interaction.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1389-1393 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Surface and interface electron states ; Conference proceedings
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    Notes: Summary We calculated the energy and the wave function of the exciton for i) a V-shaped quantum wire, ii) a T-shaped quantum wire and iii) a quantum wire resulting from strain-induced lateral confinement. The full Luttinger and Bir-Pikus Hamiltonian are taken into account in the three calculations. We used different parameters inside the well and at the barrier. The basis states |3/2m〉 are defined with respect to [110], the direction of the wire in the three kinds of structures, instead of the usual [001] direction. The hole dispersion curve allows one to define an effective mass which is to be introduced in the variational calculation of the exciton binding energy. We show that the hole is confined in a wire only in case i).
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1413-1416 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Surface and interface electron states ; Conference proceedings
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    Notes: Summary An opening (antidot) in two-dimensional (2D) electron gas placed in a strong perpendicular magnetic field makes it possible the formation of ring-shaped excitons stemming from the skipping-states of electrons and holes. The binding energy of the ground state of such excitons is logarithmically larger than that of the «bulk» 2D excitons. The possibility of tunnelling electron to hole (or vice versa) around the antidot results in a shift for each of the excitons levels that oscillates with the magnetic field.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1359-1366 
    ISSN: 0392-6737
    Keywords: Optical transient phenomena (including quantum beats, dephasings and revivals, photon echoes, free induction decay, and optical mutation) ; Excitons and related phenomena (including electron-hole drops) ; Electron states in low-dimensional structures (including quantum wells, superlattices, layer structures, and intercalation compounds) ; Weak localization effects (e.g., quantized states) ; Conference proceedings
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    Notes: Summary The optical properties of multiple quantum wells in the transition from isolated wells to a superlattice are investigated theoretically and experimentally. For superlattices with a miniband width similar to the binding energy of the 2s exciton of the isolated quantum well we find an exciton state energetically between the 1s exciton state and the onset of the miniband absorption. We find this state to be an interwell exciton, with the electron confined to one well and the hole to the neighboring well. The interwell exciton resembles the first Wannier-Stark localized exciton of a biased superlattice. However, the localization in the present case is mediated by the Coulomb interaction of the electron and hole. The state has considerable oscillator strength and is observed experimentally in linear and nonlinear experiments.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1395-1400 
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    Keywords: Excitons and related phenomena (including electron-hole drops) ; Tunneling ; Other semiconductor-to-semiconductor contacts,p-n junctions, and heterojunctions ; Electroluminescence ; Conference proceedings
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    Topics: Physics
    Notes: Summary The electroluminescence of p-i-n GaAs double-barrier resonant tunnelling diodes has been investigated for various concentrations of free carriers (either holes or electrons) in the quantum well (QW). For these structures it is possible to change the relative electron or hole density quasi-continuously with applied bias-voltage. New low-temperature excitonic recombinations are identified in the electroluminescence spectra. Continuous variation of the carrier density in the quantum well from a hole-rich into an electron-rich environment leads to the observation of positively charged excitons (X 2 +), neutral excitons (X), negatively charged excitons (X −) and finally an unbound electron-hole recombination. An increased temperature causes the dissociation of the charged excitons in favour of the neutral heavy-hole free excitonX. In high magnetic fields the unbound electron-hole recombination is transferred into an excitonic recombination.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1417-1422 
    ISSN: 0392-6737
    Keywords: Polaritons (including photon-phonon and photon-magnon interactions) ; Excitons and related phenomena (including electron-hole drops) ; Conference proceedings
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    Topics: Physics
    Notes: Summary The excitonic energies are computed in superlattices from the poles in the imaginary part of the susceptibility, using an appropriate Green's function expression for the coherent amplitude function of the electron-hole pair. Solving the Maxwell equations, the polariton states are obtained and the optical properties are computed. The procedure includes the effects of coherence between the carriers and the electromagnetic field, and it does not require any type of additional boundary conditions. Detailed calculations in GaAs/Ga1−x Al x As superlattices show polaritonic effects in the optical properties, in particular a peculiar fine-structure lineshape of then=1 andn=2 hh and lh excitons is obtained. A comparison with similar calculations in bulk GaAs clearly shows the roles of quantum confinement and of anisotropy in the superlattice.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1441-1446 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Polaritons (including photon-phonon and photon-magnon interactions) ; Optical properties of thin films, surfaces, and layer structures (superlattices, heterojunctions, and multilayers) ; II–VI compounds and other chalcogenides ; Conference proceedings
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    Notes: Summary Experimental studies of high-resolution reflectivity and a temperature dependence of the photoluminescence for periodic MQWs of CdTe/CdZnTe with periods equal to a one-half and one-forth of the light wavelength are presented. Energies and radiative lifetimes of the polariton-like excitations in the relevant structures are computed, and linked to experimental observations.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1465-1471 
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    Keywords: Surface and interface electron states ; Excitons and related phenomena (including electron-hole drops) ; Conference proceedings
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    Notes: Summary The localization of the wave function on the scale length of a single monolayer has been studied by magnetophotoluminescence in GaSb/AlGaSb quantum wells. The studied range of well width includes the direct-indirect transition involvingL-point conduction states and Γ-point valence states induced by quantum size effects. Separate carrier localization dominates at higher field values (B〉2T), whereas the excitonic effects are important only in the low field range. Variational calculations of the excitonic transverse extension provide a quantitative description of the experimental data. The dependence of the effective reduced mass on the well width has been obtained experimentally by magnetoluminescence that is highly sensitive to the modifications of the wave function, even on the scale of a single monolayer.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1505-1508 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Electron states in low-dimensional structures (including quantum wells, superlattices, layer structures, and intercalation compounds) ; III-V and II-VI inorganics ; Conference proceedings
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    Notes: Summary A model for calculating exciton binding energies in quantum wells (QWs) is presented, which can be applied to situations in which one of the two band discontinuities is large, while the second one can be arbitrary (positive, negative, or zero). The model is applied to ZnSe/ZnSSe and InGaAs/GaAs QWs., The crossover between strong-and weak-confinement, regimes for excitons in narrow QWs is studied within a simplified model with two one-parameter, variational wave functions.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1549-1553 
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    Keywords: Studies of specific magnetic materials ; Excitons and related phenomena (including electron-hole drops) ; Conference proceedings
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    Notes: Summary The influence of the electron spin splitting on the stability of quasi-two-dimensional donor bound excitons (D 0 X) has been investigated in the Cd0.97Mn0.03Te/Cd0.7Mg0.3Te semi-magnetic semiconductor quantum well (QW) structure. TheD 0 X binding energy has been measured directly from emission spectra recorded at magnetic field parallel to the QW plane. Complexes have been shown to dissociate into a neutral donor and a free exciton with a spin flip of the electron at high magnetic fields when the electron spin splitting exceeds the binding energy.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1629-1633 
    ISSN: 0392-6737
    Keywords: III–V semiconductors ; Excitons and related phenomena (including electron-hole drops) ; III–V compounds and systems ; Conference proceedings
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    Notes: Summary We report on the biexciton formation of the pseudodirect exciton consisting of the AlAs-X z electron and the GaAs-Γ heavy hole in (GaAs) m /(AlAs) m type-II superlattices withm=10, 12, and 13 monolayers. The photoluminescence lineshape of the pseudodirect exciton exhibits a doublet feature having an energy separation of ∼3 meV at the excitation power of the order of mW/cm2 in all the samples, and the low-energy band grows superlinearly. The transient profiles of the doublet photoluminescence band early indicate the biexciton formation: the delay of the rise of the biexciton photoluminescence on the low-energy side and its shorter decay time in comparison with the exciton photoluminescence.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1487-1492 
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    Keywords: III-V semiconductors ; Electron states in low-dimensional structures (including, quantum wells, superlattices, layer structures, and intercalation compounds) ; Excitons and related phenomena (including electron-hole drops) ; Brillouin and Rayleigh scattering ; Conference proceedings
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    Notes: Summary A detailed study of the relative role played by localized and/or propagating intermediate excitonic states in, resonant Rayleigh scattering (RRS) is presented for a large set of GaAs quantum well (QW) and bulk structures. We show that the two kinds of states contribute to RRS through different mechanisms. We concluded that RRS occurs via localized states in QW heterostructures, very likely due to localization by the interface roughness, while bulk, crystals turn out to be better candidates for RRS via propagating states.
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    Keywords: Surface and interface electron states ; Excitons and related phenomena (including electron-hole drops) ; Conference proceedings
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    Notes: Summary Quantum wire heterostructures, such as V- and T-shaped wires, are very promising candidates for low-threshold lasing. A crucial issue is the excitonicvs. free-carrier nature of the radiative recombination. Here, we report on magnetophotoluminescence studies of GaAs and InGaAs V-shaped wires that allow to discriminate different regimes of radiative recombination.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1601-1605 
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    Keywords: Excitons and related phenomena (including electron-hole drops) ; Polaritons (including photon-phonon and photon-magnon interactions) ; Time-resolved optical spectroscopies and other ultrafast optical measurements in condensed matter ; Conference proceedings
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    Notes: Summary Low-temperature time-resolved luminescence experiments with picosecond resolution have been performed on excitons in a microcavity containing InGaAs quantum wells. The decay curves are very dependent on the energy difference between the cavity mode and the exciton. They agree qualitatively with a model which takes into account a reservoir at the exciton energy and two splitted radiant states.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1613-1617 
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    Keywords: Excitons and related phenomena (including electron-hole drops) ; Optical properties of thin films surfaces and layer structures (superlattices, heterojunctions and multilayers) ; Conference proceedings
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    Notes: Summary We present an analysis of the inter- and intrasubband relaxation dynamics of excitons due to Fröhlich coupling in an unbiased asymmetric double quantum well made from CdTe/CdMnTe. We consider the relaxation processes that take place in the time interval between the excitation of the lowest exciton localized in the narrow well and the recombination in the wide well, by means of Fermi's golden rule. As the excitation falls in the range of a Fano resonance, we have to employ an extended set of rate equations to incorporate the effect of the continuum of initially populated states. Thus, we obtain the transient luminescence, which can directly be compared with time-resolved luminescence experiments.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1663-1667 
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    Keywords: Excitons and related phenomena (including electron-hole drops) ; III–V semiconductors ; Conference proceedings
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    Notes: Summary We calculate scattering rates of QW excitons by acoustic phonons using realistic deformation potentials for electrons and holes in structures based on GaAs. We use these rates in order to reproduce the exciton dynamics in a time-resolved photoluminescence experiment. Rise time and decay time of the luminescence signal are studied as a function of temperature and QW size. The exciton distribution function reaches a stationaryshape after the luminescence passes through its maximum. This shape shows strong deviations from the thermalequilibrium distribution, at least for temperatures below 50 K. As a consequence, exciton radiative recombination is slower than the usually expected thermal average of the radiative rate. We discuss the dependence of this effect on the well width.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1769-1774 
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    Keywords: Optical transient phenomena (including quantum beats, dephasings and revivals, photon echoes, free induction decay, and optical nutation) ; Excitons and related phenomena (including electron-hole drops) ; Electron states in low-dimensional structures (including quantum wells, superlattices, layer structures, and intercalation compounds) ; Time-resolved optical spectroscopies and other ultrafast optical measurements in condensed matter ; Conference proceedings
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    Notes: Summary We have studied experimentally the coherent exciton-photon dynamics in a single, a double, and a quintuple quantum well structure under resonant excitation. In pump and probe experiments we observe an initial fast decay of the exciton population. This initial decay depends on the number of quantum wells. The population decay is accompanied by a coherent light emission induced by the first-order polarization, the intensity of which increases quadratically with the quantum well number. Interference effects and electromagnetic coupling effects between polarizations located in distinct quantum wells probably play a crucial role in the superlinear coherent emission process.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1295-1303 
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    Keywords: Polaritons (including photon-phonon and photon-magnon interactions) ; Excitons and related phenomena (including electron-hole drops) ; Conference proceedings
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    Notes: Summary We have investigated the dynamics of impulsively excited planar microcavities in the strongly couple regime. With resonant, coherent excitation, the emitted light shows vacuum-Rabi oscillations and a lifetime corresponding to twice the cavity lifetime. As the light intensity is increased, the exciton bleaching leads to reduced normal-mode splitting. The role of interface disorder on the dynamic splitting is discussed. Coherence transfer in exciton-exciton scattering is observed. Off-resonant excitation leads to long lifetimes limited by scattering into the radiatively coupled states of the exciton-cavity system.
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  • 48
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    Keywords: Excitons and related phenomena (including electron-hole drops) ; II–VI semiconductors ; Wave propagation, transmission and absorption ; Conference proceedings
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    Notes: Summary The spectra of transmission and reflection of synthetic opal which has 3-dimensional periodic structure were measured at different orientations of incident beam relative to the sample facets. It is shown that opal behaves as «semi-metallic» photonic band gap (PBG) material in the vicinity of photon energy 2.3 eV. The synthesis of CdS microcrystals embedded in the pores of opal was made for the first time in an attempt to form a system of quantum dots. Optical spectra (reflection and transmission, photoluminescence and Raman scattering) were studied. The results demonstrate good crystallinity of microcrystals embedded in opal matrix and exhibit well-pronounced quantum confinement effects in fundamental edge absorption spectra. The spectral overlap of the PBG of opal with electronic band gap of many of II–VI semiconductors seems to make opal/semiconductor system a promising media for experimental studies of such PBG-related effects as inhibition of spontaneous emission, microcavity polariton, etc.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1377-1382 
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    Keywords: Excitons and related phenomena (including electron-hole drops) ; Time-resolved optical spectroscopies and other ultrafast optical measurements in condensed matter ; Conference proceedings
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    Notes: Summary The influence of biexciton formation on the four-wave-mixing response of multiple quantum wells having a strong inhomogeneous broadening of the exciton transitions has been investigated. The experimental results are consistent with a model which distinguishes between crystal regions where the exciton energy is almost constant over the spatial extension of the biexciton state, and regions where the fluctuations of the exciton energy are comparable to or larger than the biexciton binding energy.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1459-1463 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Other luminescence and radiative recombination ; Structural modeling: serial-addition models, computer simulation ; Conference proceedings
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    Notes: Summary We have observed an anomaly in the low-temperature photoluminescence of dipole-oriented long-lived excitons in a coupled quantum well under an electrical bias. A discrepancy between the experiment and a Monte Carlo simulation for the exciton diffusion in the system suggests a boson correlation for a two-dimensional Bose system in a random potential with repulsive interaction between bosons.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1481-1486 
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    Keywords: Excitons and related phenomena (including electron-hole drops) ; Electron states in low-dimensional structures (including quantum wells, superlattices, layers structures, and intercalation compounds) ; Optical properties of thin films, surfaces, and layer structures (superlattices, heterojunctions, and multilayers) ; Conference proceedings
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    Notes: Summary We report experimental results on photoluminescence and excitation of photoluminescence of GaAs/Ga0.7Al0.3As quantum wells (19, 38.5 and 77 Å) grown on nominal and vicinal GaAs substrates. In order to study the influence of the interface microscopic structure on the optical properties of the studied quantum wells, we perform luminescence experiments at low temperature by exciting selectively a small area of a few μm2. In this way the presence of isolated islands on the interface is evidenced by a fine structure in the photoluminescence and photoluminescence excitation spectra. The difference in localization length of excitons in samples with steps along the [110] and the [110] directions is emphasized.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1669-1673 
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    Keywords: Excitons and related phenomena (including electron-hole drops) ; Quantum Hall effect (including fractional) ; III–V semiconductors ; Conference proceedings
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    Notes: Summary Energy spectrum of an exciton and electron density distribution in it under the fractional quantum Hall regime are calculated within the framework of an anyon exciton model.
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1211-1218 
    ISSN: 0392-6737
    Keywords: Polaritons (including photon-phonon and photon-magnon interactions) ; Electrons states in low-dimensional structures (including quantum wells, superlattices, layer structures, and intercalation compounds) ; III–V and II–VI inorganics ; Excitons and related phenomena (including electron-hole drops) ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary A description of polariton effects on excitons in multiple quantum wells (MQWs), superlattices (SLs), and semiconductor microvavities is presented. The non-local semi-classical theory in a transfer matrix formulation is adopted. The radiative width in MQWs atk=0 increases first with the number of wellsN, then goes to zero asN→∞, where stationary SL polaritons are recovered. The crossover from weak- to strong-coupling regimes for QW excitons in microcavities is described. The complex energies of the mixed exciton-radiation modes are shown to be measurable by time-resolved transmission experiments.
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  • 54
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1219-1228 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Electron states in low-dimensional structures (including quantum wells, superlattices, layer structures, and intercalation compounds) ; Optical properties of thin films surfaces and layer structures (superlattices, heterojunctions and multilayers) ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary We present a comprehensive discussion of the excitonic properties of V-shaped GaAs and InGaAs quantum wires grown on patterned substrates. Systematic linear and non-linear spectroscopic studies have been performed in order to clarify the impact of lateral confinement on the exciton wave function, namely: enhanced exciton binding energy, localization in magnetic field, recombination from excited states and multiphoton absorption. The careful evaluation of the electron confinement energies, based on the actual quantum wire profile obtained by TEM micrographs, including the internal piezoelectric field induced by off-diagnoal terms of strain tensor, reproduces quite well the measured one-dimensional states. Finally, application of quantum wires in a p-i-n wave guide for bistable operation is demonstrated.
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  • 55
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1651-1656 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Polaritons (including photon-phonon and photon-magnon interactions) ; III–V semiconductors ; Cooperative phenomena ; superradiance and superfluorescence ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary We study excitons in double quantum wells (DQWs) embedded in a semiconductor microcavity (MC) by means of semi-classical theory, which yields analytic results in weak- and strong-coupling regimes. We show how to obtain subpicosecond radiative lifetime for the excitons. A model for disorder is investigated.
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  • 56
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1675-1680 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Electron states in low-dimensional structures (including quantum wells, superlattices, layer structures, and intercalation compounds) ; II–VI semiconductors ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary We discuss the exciton centre-of-mass dispersion in a (Ga,Al)As-GaAs-AlAs-(Ga, Al)As quantum well taking into account the Γ-X coupling. The position of the Γ andX quantum well levels is monitored by applying an external electric field. Near the degeneracy, the coupling plays an important role mixing the Γ andX levels. As a consequence, the exciton in-plane mass presents a significant non-parabolicity as its values change from a Γ-reduced mass to aX-reduced mass.
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  • 57
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1229-1237 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Electron states in low-dimensional structures (including quantum wells, superlattices, layer structures, and intercalation compounds) ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary We consider in this work the scattering of low-energy excitons in single quantum wells. In the first part we consider the elastic scattering by a static centre (charged impurity) and discuss the validity of the first Born approximation, which is expected to work well only for particles with large kinetic energy. The second part is devoted to the inelastic scatterings of individual excitons by a phonon reservoir. We discuss in particular the validity of the elastic approximation, which is well known to be very accurate for particles with large kinetic energy.
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  • 58
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1239-1245 
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; III–V semiconductors ; Photoluminescence ; Metal-insulator transitions ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary We implement optical spectroscopy to study charged excitons (trions) in modulation-doped GaAs/AlGaAs quantum wells. We observe for the first time several new trions: the positively charged exciton, the light-hole negatively charged exciton and the triplet state of the negatively charged exciton.
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  • 59
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1713-1716 
    ISSN: 0392-6737
    Keywords: Polaritons (including photon-phonon and photon-magnon interactions) ; Electron states in low-dimensional structures (including quantum wells, superlattices, layer structures, and intercalation compounds) ; III–V and II–VI inorganics ; Excitons and related phenomena (including electron-hole drops) ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary A quantum-mechanical model for quantum well polaritons in semiconductor microcavities is presented. The real and imaginary parts of the polariton dispersion are obtained. They represent, respectively, the position and the linewidth of the luminescence peaks observed in typical experiments. The model includes the features of the distributed Bragg reflectors which constitute the cavity mirrors, and has the advantage of describing the weak-coupling as well as the strong-coupling regimes characteristic of this kind of systems. Furthermore, in the limit where no cavity is present, the well-known result for quantum well polaritons is recovered.
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  • 60
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1735-1741 
    ISSN: 0392-6737
    Keywords: Wave propagation, transmission and absorption ; Optical properties of thin films, surfaces and layer structures (superlattices, heterojunctions, and multilayers) ; Excitons and related phenomena (including electron-hole drops) ; Time-resolved optical spectroscopies and other ultrafast optical measurements in condensed matter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary It is shown that collective effects determine the optical response of radiatively coupled multiple-quantum-well structures. Stimulated decay of the coherent electronic excitations is predicted for systems where the periodicity unit is an integer multiple of half the optical wavelength. For quarter wavelength structures it is shown that the induced optical coupling causes an interwell energy transport and a splitting of the excitonic resonances leading to quantum beats in the time-resolved transmitted and reflected signal.
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  • 61
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    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1759-1762 
    ISSN: 0392-6737
    Keywords: Optical transient phenomena (including quantum beats, dephasings and revivals, photon echoes, free induction decay, and optical mutation) ; Excitons and related phenomena (including electron-hole drops) ; Time-resolved optical spectroscopies and other ultrafast optical measurements in condensed matter ; Optical properties of thin films, surfaces and thin layer structures (including superlattices, heterostructures, and intercalation compounds) ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary We study exciton ionization induced by an axial electric field in a strongly coupled GaAs/Al0.3 Ga0.7 As superlattice at low temperatures. The field-induced ionization times of the heavy-hole 1s exciton in the miniband field regime are determined from transient four-wave-mixing experiments and theoretical model calculations. They are found to lie between the field ionization times of excitons in bulk semiconductors, and in strongly confined quantum well systems.
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  • 62
    ISSN: 1432-2145
    Keywords: Embryo sac ; Zea mays ; Enzymatic isolation ; Zygotic embryogenesis ; Microinjection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The long-term viability of isolated embryo sacs was studied in maize. Fertilised embryo sacs were digested in order to remove most of the nucellus cells present on their surfaces and then transferred to culture. Experiments on 161 embryo sacs showed that isolation treatments using even minimal enzymatic digestion affected the further development of the embryo sacs. Few embryo sacs survived in culture and those produced only abnormal embryos; they produced no plants. We concluded that embryo sacs isolated through enzymatic digestion may offer limited prospects for long-term studies where normal embryogenic development is required. Alternative strategies are discussed for maize.
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  • 63
    ISSN: 1432-2145
    Keywords: Zea mays ; Calcium ; Cell integrity ; Cell viability ; Sperm cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Our previous studies showed that Brewbaker and Kwack salts, which have been widely used in pollen germination and sperm isolation, are not appropriate for the maintenance of isolated maize (Zea mays L.) sperm cells. In the present study, we have characterized the effects of each BKS component salt on the integrity of isolated sperm cells using hemacytometry. At 0.01 and 0.1 mM, there were no differences in cell number between control and any salt-treated cells except a 22% decrease with 0.1 mM MgSO4 at 48 h. At the 1 mM level, cell number decreased with time in the presence of Ca(NO3)2 and MgSO4, with loss of integrity of most cells at 48 h, while KNO3 and H3BO3 had little or no effect. Further characterization of calcium-induced reduction in cell integrity using flow cytometry showed that depletion of possible residual free calcium by addition of EGTA to the suspension medium improved cell longevity and viability. Exposure of isolated sperm cells to 1 mM calcium had no effect on cell integrity and viability in 5 h; however, only 12% of cells remained intact at 24 h. The reduction in cell integrity was hastened when cells were pretreated with the calcium ionophore A23187 prior to exposure to 1 mM calcium, with a 54% reduction in cell number at 1 h and complete cell lysis at 24 h. However, depletion of cytosolic free calcium by pretreatment of cells with the calcium ionophore followed by resuspension in the presence of EGTA resulted in rapid reduction of cell integrity as well. These results collectively suggest that maize sperm cells are sensitive to exogenous free calcium; however, a certain level of cytosolic free calcium is necessary for maintenance of integrity. Mechanisms of calcium-induced reduction in cell integrity are discussed along with possible roles of the sensitivity of sperm cells to calcium in fertilization.
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  • 64
    ISSN: 1573-5028
    Keywords: GTP binding ; ADP ribosylation ; Zea mays ; Escherichia coli ; fatty acid biosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In an attempt to isolate a plant malonyl-coenzyme A:acyl carrier protein transacylase cDNA clone, by direct genetic selection in an Escherichia coli fabD mutant (LA2-89) with a maize cDNA expression library, a Zea mays cDNA clone encoding a GTP-binding protein of the ARF family was isolated. Complementation of a mutation affecting bacterial membrane lipid biosynthesis by a plant ARF protein, could indicate the existence of as yet unidentified bacterial equivalents of this ubiquitous eucaryotic GTP-binding protein.
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  • 65
    ISSN: 1573-5028
    Keywords: β-prolamin ; Coix lacryma-jobi ; different O2-binding sites ; Opaque 2 ; transcriptional regulation ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The maize opaque 2 (o2) mutation is known to have numerous pleiotropic effects. Some polypeptides have their expression depressed while others are enhanced. The best characterized effects of the o2 mutation are those exerted on endosperm genes encoding the storage protein class of the 22 kDa α-zeins and the ribosome inactivating protein b-32. The Opaque 2 (O2) locus encodes a basic domain-leucine zipper DNA-binding factor, O2, which transcriptionally regulates these genes. In the maize-related grass Coix lacryma-jobi, an O2-homologous protein regulates the 25 kDa α-coixin gene family. We show in this paper that O2 transcriptionally regulates the structurally and developmentally different class of the β-prolamins. A new O2-binding box was identified in β-prolamin genes from maize and Coix that, together with the boxes previously identified in other endosperm expressed genes, forms a curious collection of O2 cis elements. This may have regulatory implications on the role of O2 in the mechanism that controls coordinated gene expression in the developing endosperm. Considering that the O2 locus controls at least three distinct classes of genes in maize endosperm, we propose that the O2 protein may play a more general role in maize endosperm development than previously conceived.
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  • 66
    ISSN: 1573-5028
    Keywords: elongation factor 1α ; EF-1α ; Zea mays ; cDNA sequence ; gene
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    Topics: Biology
    Notes: Abstract A cDNA (zmEF1A) and the corresponding genomic clone (zmgEF1A) of a member of the gene family encoding the α subunit of translation elongation factor 1 (EF-1α) have been isolated from maize. The deduced amino acid sequence is 447 residues long interrupted by one intron. Southern blot analysis reveals that the cloned EF-1α gene is one member out of a family consisting of at least six genes. As shown by northern hybridizations in leaves the mRNA level increases at low temperature whereas time-course experiments over 24 h at 5°C show that in roots the overall mRNA level of EF-1α is transiently decreased. These results indicate that the expression of EF-1α is differently regulated in leaves and roots under cold stress.
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  • 67
    ISSN: 1573-5028
    Keywords: anaerobiosis ; BMS cells ; glyceraldehyde-3-phosphate dehydrogenase ; transient gene expression ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cytosolic glyceraldehyde-3-phosphate dehydrogenase (GapC) gene family of maize is differentially expressed in response to anaerobic stress. While GapC1 and GapC2 are downregulated, GapC3 and GapC4 are anaerobically induced. We have sequenced and analyzed a 3073 bp promoter fragment of GapC4. The promoter confers anaerobic induction of a reporter gene construct in a transient gene expression system in maize. Deletion analysis of the GapC4 promoter revealed a 270 bp long DNA region required for anaerobic induction. This region contains sequence motifs resembling the cis-acting sequences of the anaerobically induced maize Adh1 and Adh2 genes. Furthermore, the 3073 bp GapC4 promoter fragment displays homology to long terminal repeats of maize retrotransposons and to the 3′ region of the maize anthocyanin regulatory locus C1.
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  • 68
    ISSN: 1573-5028
    Keywords: carbon catabolite repression ; cDNA ; gene expression ; stress-induced genes ; glucose-starvation ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to isolate glucose-starvation-related cDNAs in maize (Zea mays L.) root tips, a cDNA library was constructed with poly(A)+ mRNA from 24 h starved root tips. After differential screening of the library, we isolated six different cDNAs (named pZSS2 and pZSS7) which were expressed during glucose starvation. Time course analysis revealed that maximum expression of five of these genes occurs 30 h after the onset of the starvation treatment. On the contrary, the expression of mRNAs corresponding to pZSS4 was maximal at an early stage of starvation and then dramatically decreased. The expression of this gene did not seem to be specific for glucose starvation. The pattern of induction of the genes corresponding to pZSS2, pZSS3, pZSS5, pZSS6 and pZSS7 revealed that non-metabolizable sugars such as L-glucose and mannitol induce mRNA transcription similarly to glucose starvation. When D-glucose or any other metabolizable sugar was supplied, the level of transcripts was reduced. Nucleotide sequence analyses of the six cDNAs allowed identification of five of them by comparison with sequence data bases. The protein encoded by clone pZSS2 is analogous to a wound-induced protein from barley. Clones pZSS4 to pZSS7 encode, respectively, a transmembrane protein, a cysteine protease, a metallothionein-like protein and a chymotrypsin/subtilisin-like protease inhibitor. Clone pZSS3 shares no significant homology with any known sequence.
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  • 69
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    Plant molecular biology 28 (1995), S. 667-676 
    ISSN: 1573-5028
    Keywords: gene expression ; promoter ; glyceraldehyde-3-phosphate dehydrogenase ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transient expression experiments show that the maize GapA1 promoter exhibits a requirement for sequences contained within intron 1 and surrounding exon border regions for expression in maize Black Mexican Sweet cells. Maize GapA1-promoter constructs lacking intron 1 are inactive. Intron 1 and its exon border sequences, when reintroduced into constructs lacking introns, restore gene activity whereas intron 2 and its exon borders to not. The minimal promoter so defined encompasses roughly 250 bp upstream of the in vivo transcription start and appears also to include intron 1. An octameric sequence was identified in intron 1 of maize GapA1 which is similar to sequence motifs found in other maize introns known to increase transient expression. Partial restoration of gene expression in GapA1 constructs lacking intron 1 was achieved through insertion of the identified octameric sequence.
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  • 70
    ISSN: 1573-5028
    Keywords: calmodulin ; cDNA library ; embryogenesis ; PCR ; transition stage ; Zea mays
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    Notes: Abstract One hundred maize zygotic embryos microdissected at the transition stage were used to construct a cDNA library after non-selective PCR (NS-PCR) amplification of whole cDNA populations. The library contains 2.3 × 105 recombinants and two different calmodulin cDNAs were cloned using a heterologous probe from petunia. Calmodulin expression was confirmed throughout maize embryogenesis at the mRNA, amplified cDNA and protein levels. Sequence analysis suggests a maize origin for both clones and negligible nucleotide changes linked to PCR. This library is the first described for early plant embryos and represents a breakthrough to isolate genes involved in embryo differentiation.
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  • 71
    ISSN: 1573-5028
    Keywords: Zea mays ; thiamine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thiamine or vitamin B-1, is an essential constituent of all cells since it is a cofactor for two enzyme complexes involved in the citric acid cycle, pyruvate dehydrogenase and α-ketoglutarate dehydrogenase. Thiamine is synthesized by plants, but it is a dietary requirement for humans and other animals. The biosynthetic pathway for thiamine in plants has not been well characterized and none of the enzymes involved have been isolated. Here we report the cloning and characterization of two cDNAs representing members of the maize thi1 gene family encoding an enzyme of the thiamine biosynthetic pathway. This assignment was made based on sequence homology to a yeast thiamine biosynthetic gene and by functional complementation of a yeast strain in which the endogenous gene was inactivated. Using immunoblot analysis, the thi1 gene product was found to be located in a plastid membrane fraction. RNA gel blot analysis of various tissues and developmental stages indicated thi1 expression was differentially regulated in a manner consistent with what is known about thiamine synthesis in plants. This is the first report of cDNAs encoding proteins involved in thiamine biosynthesis for any plant species.
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  • 72
    ISSN: 1432-2048
    Keywords: Anoxia ; Biochemical pH-stat ; Cytoplasmic pH ; Ethanol production ; Pyruvate decarboxylase ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ethanol production by maize (Zea mays L.) root tips, measured by an enzymic assay of the suspending medium, was correlated with changes in the cytoplasmic pH, determined by in-vivo 31P nuclear magnetic resonance (NMR) spectroscopy, following the onset of anoxia. Strong evidence for the role of the cytoplasmic pH in triggering the switch to ethanol production under anoxia was obtained by: (i) varying the pH of the suspending medium between pH 4 and pH 10; and (ii) using the permeant weak base methylamine to combat the acidification of the cytoplasm induced by the anoxic conditions. Experimentally, it proved to be much easier to manipulate the cytoplasmic pH under anoxia after the pH had stabilised, rather than during the initial rapid acidification that occurred following the onset of anoxia, and in the presence of methylamine, it was possible to impose a normal aerobic cytoplasmic pH value on tissue that was metabolising anaerobically. By this means it was possible to demonstrate the reversibility of the pH effect on ethanol production under anoxia and thus to provide good evidence in support of the biochemical pH-stat model of the anoxic response. The NMR measurement of the cytoplasmic pH in the presence of methylamine was achieved by using a manganese pretreatment technique to eliminate interference between the cytoplasmic and vacuolar Pi signals, and it seems likely that the experimental approach used here will have further applications in studies of the metabolic response to anoxia.
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  • 73
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    The journal of membrane biology 143 (1995), S. 19-28 
    ISSN: 1432-1424
    Keywords: Plant membrane ; Lipid domain ; Fluorescence photobleaching recovery ; Electron paramagnetic resonance ; Temperature stress ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Translational diffusion of a fluorescent sterol probe was measured in the plasma membranes of protoplasts isolated from cortical cells of the primary root of maize seedlings. The apparent lateral diffusion coefficient was typically observed to be nearly insensitive to temperature, while the mobile fraction increased with increasing temperature. These fluorescence photobleaching recovery (FPR) measurements were compared with the electron paramagnetic resonance (EPR) spectra of the methyl ester of 13-doxyl palmitic acid in membranes of corn root tissue in situ. The complex spectra observed with this probe were analyzed as weighted sums of simpler spectra of various order parameters and rotational correlation times. The reconstituted spectra calculated from the model show that EPR also detects a mobile (less ordered, fluid) fraction, distinguished by the order parameter S=0.1 to 0.2, which becomes more abundant as temperature increases and is qualitatively comparable to the mobile fraction determined by the FPR method. The observed results on the mobile fractions and the diffusion rates for translational (FPR) as well as rotational (EPR) motions are interpreted in terms of membrane organization, thus providing information on the population and structural patterns of the coexisting domains with a special emphasis on the response of the membrane to temperature changes.
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  • 74
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    Theoretical and applied genetics 90 (1995), S. 1198-1203 
    ISSN: 1432-2242
    Keywords: Diplospory ; RFLP ; Bulk-segregant analysis ; Genome similarity ; Intergeneric hybrids ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polyploid plants in the genus Tripsacum, a wild relative of maize, reproduce through gametophytic apomixis of the diplosporous type, an asexual mode of reproduction through seed. Moving gene(s) responsible for the apomictic trait into crop plants would open new areas in plant breeding and agriculture. Efforts to transfer apomixis from Tripsacum into maize at CIMMYT resulted in numerou intergeneric F1 hybrids obtained from various Tripsacum species. A bulk-segregant analysis was carried out to identify molecular markers linked to diplospory in T. dactyloides. This was possible because of numerous genome similarities among related species in the Andropogoneae. On the basis of maize RFLP probes, three restriction fragments co-segregating with diplospory were identified in one maize-Tripsacum dactyloides F1 population that segregated 1∶1 for the mode of reproduction. The markers were also found to be linked in the maize RFLP map, on the distal end of the long arm of chromosome 6. These results support a simple inheritance of diplospory in Tripsacum. Manipulation of the mode of reproduction in maize-Tripsacum backcross generations, and implications for the transfer of apomixis into maize, are discussed.
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  • 75
    ISSN: 1615-6102
    Keywords: Drosera rotundifolia ; Embryogenic callus ; Extracellular matrix ; Scanning electron microscopy ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Primary embryogenic callus ofDrosera rotundifolia and long-term cultured embryogenic callus ofZea mays possess a conspicuous extracellular matrix (ECM) around and between embryogenic cells. The structural arrangement of ECM depends on the developmental stage of the embryogenic cells. Single embryoid cells were covered with, and connected by net-like material. However, surface cells of young globular embryoids were covered with a coherent layer of ECM which forms bridges with net-like material between the cells which was gradually reduced to coarse strands. When protodermis was formed on the surface of globular embryoids, the ECM disappeared completely. The ECM network was never observed on the surface of heart- and torpedo-shaped embryoids. Safranine (especially 0.1%) stabilized the structure of ECM. Digestion with pronase E and proteinase K indicated that the ECM contains proteinaceous components. Similar developmental patterns of ECM were observed in dicotyledonous and monocotyledonous examples. The ECM represents a stable morphological structure even during long-term embryogenic culture in maize.
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  • 76
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    Protoplasma 187 (1995), S. 3-12 
    ISSN: 1615-6102
    Keywords: Gamete protoplasts ; In vitro fertilization ; Early embryogenesis ; Developmental mutations ; Polymerase chain reaction ; cDNA libraries ; Gene cloning ; Angiosperm ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The development of in vitro fertilization methods in plants, the characterization of developmental mutants, and the adaptation of molecular biology techniques to construct cDNA libraries from minute samples, all represent important recent technical break-throughs. They allow the study of fertilization and early embryogenesis at a molecular level and considerable improvement in the under-standing of higher plant reproduction can be predicted over the next few years. Important biological questions, such as polyspermy, gamete fusion physiology, asymmetrical cell division, embryo axis formation, can now be addressed experimentally in maize, which appears as a major study model in this area.
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  • 77
    ISSN: 1615-6102
    Keywords: Biolistics ; Microspores ; GUS-expression ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ability to recover male gametophyte derived plants, which is necessary to get transformed haploid plants, was verified for a hybrid of maize. Using the isolated microspore culture technique, a 9 × 10−5 plant regeneration frequency was obtained. Maize microspores were bombarded with tungsten particles using a PDS He/1000 apparatus. GUS expression in the microspores was maximum with 1.1 μm diameter tungsten microprojectiles for 1100 and 1350 psi helium pressures at a 6 cm distance between the launch point and the target cells. Increasing the amount of DNA coated on the microparticles from 1.66 to 4 μg DNA/mg of particles allowed a two-fold and four-fold increase of the GUS-expressing microspore frequency for 1100 and 1350 psi helium pressure bombardment, respectively. Optimal concentration of solidifying agent in the bombardment support culture medium was found to be 1%. Cell density ranging from 25000 microspores/bombardment to 100000 microspores/bombardment did not affect the frequency of GUS-expressing microspores. Using these optimal conditions, the maximum frequency of GUS-expressing microspores was found to be about 9 × 10−4, while maintaining an embryo formation frequency about 5 × 10−4.
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  • 78
    ISSN: 1615-6102
    Keywords: Auxin ; Cell wall ; Coleoptile ; Pectin ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Aiming to elucidate the possible involvement of pectins in auxin-mediated elongation growth the distribution of pectins in cell walls of maize coleoptiles was investigated. Antibodies against defined epitopes of pectin were used: JIM 5 recognizing pectin with a low degree of esterification, JIM 7 recognizing highly esterified pectin and 2F4 recognizing a pectin epitope induced by Ca2+. JIM 5 weakly labeled the outer third of the outer epidermal wall and the center of filled cell corners in the parenchyma. A similar labeling pattern was obtained with 2F4. In contrast, JIM 7 densely labeled the whole outer epidermal wall except the innermost layer, the middle lamellae, and the inner edges of open cell corners in the parenchyma. Enzymatic de-esterification with pectin methylesterase increased the labeling by JIM 5 and 2F4 substantially. A further increase of the labeling density by JIM 5 and 2F4 and an extension of the labeling over the whole outer epidermal wall could be observed after chemical de-esterification with alkali. This indicates that both methyl- and other esters exist in maize outer epidermal walls. Thus, in the growth-controlling outer epidermal wall a clear zonation of pectin fractions was observed: the outermost layer (about one third to one half of wall thickness) contains unesterified pectin epitopes, presumably cross-linked by Ca2+ extract. Tracer experiments with3H-myo-inositol showed rapid accumulation of tracer in all extractable pectin fractions and in a fraction tightly bound to the cell wall. A stimulatory effect of IAA on tracer incorporation could not be detected in any fraction. Summarizing the data a model of the pectin distribution in the cell walls of maize coleoptiles was developed and its implications for the mechanism of auxin-induced wall loosening are discussed.
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  • 79
    ISSN: 1615-6102
    Keywords: Chromosome endoreduplication ; Endosperm ; Protein accumulation ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chromosome endoreduplication is a very common process in higher plants but its function and genetic control are still to be clarified. In our experiments we analyzed, by Feulgen cytophotometry, chromosome endoreduplication in endosperm cells of two maize genotypes, IHP and ILP, having high and low protein content in their seed, respectively. Chromosome endoreduplication occurs in both lines within 24 days after pollination, attaining a maximum ploidy level of 384C (7 DNA replication rounds) in IHP and of 192C (6 replication rounds) in ILP. In the mature seed, endosperms of the two lines show different mean ploidy level. In reciprocal crosses between IHP and ILP the f1 endosperms have mean ploidy levels analogous to that of the maternal parent, showing that the difference in ploidy level between the two genotypes is maintained. After selfing of the f1 plants, the difference in ploidy level between the two F2 populations is reduced. In F2 the mean ploidy level is as variable as in f1, indicating the absence of genetic segregation. From our data, it is apparent that both the genetic constitution (cytoplasmic and nuclear) of the maternal parent and the genotype of the individual endosperms influence the ploidy level. An analysis of the protein content in endosperms carried out on the same seed sample as analyzed cytophotometrically showed that the protein content increases, during seed development, parallel to chromosome endoreduplication and varies, in the two lines, in reciprocal crosses and their progeny, according to the same trend as mean ploidy level, suggesting a correlation between the two parameters.
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  • 80
    ISSN: 1615-6102
    Keywords: Avena sativa ; Elongation growth ; Glycine max ; Hexacyanoferrate III (ferricyanide) ; Indole-3-acetic acid ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The influence of exogenous potassium hexacyanoferrate (III) (HCF III) on elongation of maize (Zea mays L.) coleoptile segments was investigated. Addition of HCF III led to a strong stimulation of growth both in the presence and absence of indole-3-acetic acid (IAA). The magnitude of growth stimulation was dependent on the presence of IAA, HCF III concentration, incubation time, and phase growth. The reduced form, potassium hexacyanoferrate (II), was without effect on growth. In the presence of HCF III, elongation was suppressed when coleoptile segments were treated with N,N′-dicyclohexylcarbodiimide, cycloheximide or atebrine (quinacrine). The addition of HCF III stimulated the IAA-induced proton extrusion, and the e−/H+ ratio decreased with incubation time. HCF III also strongly stimulated elongation ofAvena saliva L. coleoptile segments andGlycine max L. hypocotyl segments. These results suggested that a plasma membrane redox system (NADH oxidase type I) may be involved in the regulation of growth through the activity of the plasma membrane-bound ATPase.
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  • 81
    ISSN: 1615-6102
    Keywords: Zea mays ; Plasma membrane ; Proton microprobe ; Copper ; Iron ; Zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plasma membranes of maize (Zea mays L., cv. Sil Anjou 18) roots were isolated by aqueous two-phase partitioning. Multi elemental analysis by proton induced X-ray emission (PIXE) was used for the investigation of elemental composition of plasma membranes. Fe, Cu, and Zn as well as P, S, and Ca were identified. We did not find significant amounts of V, Mn, Se, Mo, or W.
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  • 82
    ISSN: 1615-6102
    Keywords: Glutathione ; Plasma membrane redox acitvity ; Electron transfer ; Zea mays ; Diamide ; Buthionine-D,L-suIfoximine ; Hexacyanoferrate III ; Hexabromoiridate IV
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We investigated changes of thiols (GSH, GSSG, and cysteine) induced by transplasma membrane electron transport after addition of artificial electron acceptors and the influence of the thiol level on redox activity. GSH, GSSG, and cysteine content of maize (Zea mays L. cv. Golden Bantam) roots and coleoptile segments was determined by high performance liquid chromatography with a fluorescence detector. GSSG increased after treatment with 0.8 mM diamide, an SH-group oxidizer. GSH level of roots increased after treatment with diamide, while GSH levels of coleoptiles decreased. Incubation of roots with the GSH biosynthesis inhibitor buthionine-D,L-sulfoximine for 6 days lowered the glutathione level up to 80%. However, the GSH/GSSG ratio of maize roots remained constant after treatment with both effectors. The GSH/GSSG ratio and the glutathione level were changed by addition of artificial electron acceptors like hexacyanoferrate (III) or hexabromoiridate (IV), which do not permeate the plasma membrane. Hexacyanoferrate (III) reduction was inhibited up to 25% after the cellular glutathione level was lowered by treatment with diamide or buthionine-D,L-sulfoximine. Proton secretion induced by reduction of the electron acceptors was not affected by both modulators. The change in glutathione level is different for roots and coleoptiles. Our data are discussed with regard to the role of GSH in electron donation for a plasma membrane bound electron transport system.
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  • 83
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    Molecular genetics and genomics 246 (1995), S. 716-722 
    ISSN: 1617-4623
    Keywords: α-tubulin gene ; DNA methylation ; Endosperm ; Parental imprinting ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have investigated the methylation status of the α-tubulin genes, and the degree of accumulation of their mRNAs in endosperm, embryo and seedling tissues of Zea mays L. We have found that many of the α-tubulin genes are differentially demethylated in the endosperm relative to the embryo and seedling. However, only for tubα2 and tubα4 could a correlation between DNA demethylation and increased RNA accumulation be detected. By analyzing the inbred lines W64A and A69Y and their reciprocal crosses, we have also identified in the endosperm two α-tubulin genes, tubα3 and tubα4, that are differentially demethylated if transmitted by the maternal germline, but that remain hypermethylated when transmitted by the paternal germline.
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  • 84
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    Molecular genetics and genomics 247 (1995), S. 255-263 
    ISSN: 1617-4623
    Keywords: Zea mays ; Mitochondrial genome ; Pulsed-field gel electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pulsed-field gel electrophoresis (PFGE) in combination with infrequently cutting restriction enzymes was used to investigate the structure of the mitochondrial (mt) genome of the maize variety Black Mexican Sweet (BMS). The mt genome of this variety was found to resemble that of the closely related B37N variety, with one recombination and five insertion/deletion events being sufficient to account for the differences observed between the two genomes. The majority of the BMS genome is organized as a number of subgenomic chromosomes with circular restriction maps. Several large repeated sequences are found in the BMS mt genome, but not all appear to be in recombinational equilibrium. No molecules large enough to contain the entire mt genome were discernible using these techniques. The mapping approach described here provides a means of quickly analyzing the large and complex rut genomes of plants.
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  • 85
    ISSN: 1617-4623
    Keywords: Zea mays ; Transposable elements ; Unstable mutation ; Variegation pattern ; Modifier gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Ac elements present in the unstable wxm7 and wx-m9 alleles of maize trigger different patterns of Ds excision in trans. To determine whether this differential regulation is a feature of the Ac alleles themselves or is mediated by genetically distinct factors, maize plants heterozygous for the wx-m7 and wx-m9 alleles were crossed to tester strains homozygous for Ds reporter alleles. Kernels showing the variegation pattern characteristic for the Ac elements carried in the wx-m7 and wx-m9 alleles were found to be present in the ratios expected from the genetic constitution of the strains. The aleurone variegation caused by excision of the Ds reporter element and the endosperm variegation caused by excision of Ac from the wx-m7 and wx-m9 alleles themselves segregated with the original wx-m alleles. In addition, stable Wx and wx derivatives of wx-m9 that have lost Ac no longer exert any trans effect on the wx-m7 allele (and vice versa). Therefore it is concluded that the observed variegation patterns are autonomously determined by specific trans effects of the particular Ac element.
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  • 86
    ISSN: 1573-5036
    Keywords: cadmium ; root distribution ; root methods ; soil pollution ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Cadmium uptake by maize from polluted river sediments covered with a clean top layer of variable thickness is discussed in relation to root distribution. Two pathways for uptake are distinguished: roots penetrating the contaminated layer or contaminants moving into the root zone. Relative Cd uptake proved to be roughly proportional to the fraction of total root length found in the contaminated layer. A deeper water table induced a deeper root development and more Cd uptake for a given thickness of clean topsoil. A model based on exponential decrease of root length density with depth is acceptable as first approximation only. Little or no evidence was found for contaminants moving into the root zone during the ten years of the experiment.
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  • 87
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    Euphytica 82 (1995), S. 157-164 
    ISSN: 1573-5060
    Keywords: EPSP synthase ; glyphosate ; herbicide tolerance ; natural variation ; Zea mays ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Variation in susceptibility to the safe broad-spectrum herbicide glyphosate was investigated in maize. Eleven inbred lines, grown in a growth chamber, were evaluated for their tolerance to the herbicide at 2.4 mM (0.2 kg a.i. in 400 I ha-1 of water). Following treatment with glyphosate at the three-leaf stage, significant variation in damage, expressed as visual injury ratings scored 7, 14 and 21 days after the application of the herbicide, was found. Effects on dry weight and shoot height were consistent with visual scores and the carbon-exchange rate was found to be a sensitive index of differential injury. Biochemical characterization of 5-enol-pyruvyl-shikimate-3-phosphate (EPSP) synthase, the main target of the herbicide, ruled out the possibility that this differential susceptibility was due to variations in the sensitivity of the enzyme. On the contrary, a positive correlation was found between in vivo tolerance and EPSP synthase levels, measured at different stages during seedling growth. This result suggests that a naturally occurring difference in EPSP synthase levels in the tissues may contribute to the differential response observed in vivo in maize inbreds.
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  • 88
    ISSN: 1573-5060
    Keywords: Zea mays ; maize, subtropical white germplasm ; S3 recurrent selection ; inbreeding depression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In maize (Zea mays L.) source germplasm having tolerance to inbreeding is needed for the derivation of productive inbred lines. The germplasm with non-temperature adaptation, generally suffer from large inbreeding depression (ID). the objective of the present study was to improve four white subtropical populations for tolerance to ID through S3 recurrent selection. Two cycles of selection were conducted and the effect of selection was evaluated in the first selfed (S1) and random0mated (Synthetic-2=Syn-2) generations. To complete each cycle. 200 to 250 S3 progenies were evaluated and 15 to 20 superior ones selected for recombination in each population. Selection was practiced for high grain yield, vigour and other agronomie traits. The S1 and Syn-2 generations of the original (C0) and improved (C1, C2) populations were evaluated in a split-split plot design, with populations in main plots, generations in subplots and cycles in sub-subplots. In all populations, S1 and Syn-2 generations of C2 were significantly higher yielding than the C0 and the linear gain per cycle varied from 16.3 to 28.8% in S1 generation and 5.5 to 10.7% in Syn-2 generation. Selection reduced the anthesis-silking interval but slightly increased plant height, grain moisture and days to silk. An increase in plant height was expected because of emphasis on vigour during selection. For grain yield. ID decreased from 48.4% in C0 populations to 37.9% in C2, indicating an improved tolerance to inbreeding. Further, the selection accumulated favourable alleles; and Pop. 42 seemed to be the best source populations for deriving high yielding inbred lines. It may be concluded that S3 recurrent selection was effective in improving grain yield and the improved populations would provide better germplasm sources for the derivation of productive inbred lines.
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  • 89
    ISSN: 1573-5036
    Keywords: aluminium toxicity ; Eucalyptus camaldulensis ; fulvic acid ; humic acid ; root elongation ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Complexes of aluminium (Al) with organic ligands are believed to represent an important detoxification mechanism in acid soils. However, relatively little is known about the particular ligands produced by decomposing vegetation or about their effects on plant growth in the presence or absence of toxic Al. This paper reports an experiment on the effects of decomposition products of Eucalyptus camaldulensis leaves on the root elongation of maize (Zea mays) cv. DK687 in the presence or absence of Al. The static solution culture experiment used fulvic acid (FA) and humic acid (HA), extracted from E. camaldulensis leaves, at three nominal concentrations, viz. 40, 120 and 360 mg C L-1, replicated 4 times in the presence and absence of 30 µM Al. In the absence of Al, root elongation was increased by 30% by HA at 40 mg C L-1 and by 36% by FA at 120 mg C L-1. In the presence of 30 µM Al, the effects of toxic Al on root elongation were negated by FA and HA at all concentrations. Aluminium was totally complexed in all treatments except FA at 40 mg C L-1 in which treatment only 2.7 µM Al was present in the monomeric form. The E. camaldulensis FA and HA at concentrations of 40 and 120 mg C L-1, either in the presence or absence of Al, stimulated maize root elongation. Aluminium was strongly complexed by the E. camaldulensis FA and HA. The present results, in which FA and HA alleviated Al toxicity limitations on root elongation of maize, are relevant to the protection afforded to plant growth in acid soils amended with organic materials. They highlight the need to focus more on the role of FA and HA.
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  • 90
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    Plant and soil 175 (1995), S. 233-238 
    ISSN: 1573-5036
    Keywords: arbuscular mycorrhiza (AM) ; Cd ; Glomus mosseae ; phytotoxicity ; tolerance ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A Cd-tolerant arbuscular mycorrhizal fungus, Glomus mosseae, isolated from a polluted soil (P2 culture), was compared with a Cd-sensitive reference Glomus mosseae (Gm) for its capacity to colonize maize (Zea mays L.) roots and to affect plant growth and Cd uptake in sand culture at increasing exposure to Cd added with the nutrient solution (0, 0.1, 1, 5 and 10 mg L-1). After eight weeks, mycorrhizal colonization by P2 culture was relatively high (50% of the control without Cd added) up to 5 mg L-1 Cd, whereas colonization by Gm was almost totally suppressed at that Cd level. However, even mycorrhizal colonization by the Cd-tolerant P2 culture appeared more sensitive to Cd than plant growth and was completely suppressed at 10 mg L-1 Cd. AM colonization did not alleviate the negative effect of Cd on plant growth compared to the non-mycorrhizal treatment. On the contrary, at the 5 mg L-1 Cd level non-mycorrhizal plants were greater than mycorrhizal plants with lower Cd concentration.
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  • 91
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    Plant cell, tissue and organ culture 43 (1995), S. 123-126 
    ISSN: 1573-5044
    Keywords: bioreactor ; dissolved oxygen ; haploid cell suspension ; pH ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Maize (Zea mays L.) haploid cells were cultivated in a 1500 ml aerated and stirred batch bioreactor using modified BM medium. Cell growth was highly affected by pH and dissolved oxygen, and we observed two fairly distinct growth phases. During the first two days after inoculation at pH 5.8, oxygen consumption was high and the cells lowered the pH to a value around 4.3. After this period the pH stabilized at 4.5 and the dissolved oxygen reached a steady level. Decreasing dissolved oxygen concentration leads to lower growth rate and to higher pH. Both events mean stress conditions for the cell culture and probably result in increased genetic variability, and the loss of regeneration capacity. The stress condition during the adaptation phase can be eliminated by decreasing the pH of the medium to 4.7 before inoculation and by keeping dissolved oxygen above 40%. These conditions provide prolonged exponential growth dynamics and the cell suspensions could be the basis of large scale cultures also.
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  • 92
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    Plant cell, tissue and organ culture 42 (1995), S. 147-152 
    ISSN: 1573-5044
    Keywords: corn ; kernel culture ; light quality ; phytochrome ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Growth and development of plants are known to be affected by exposure to red and blue light. Mechanisms by which light quality influences gene expression in maize (Zea mays L.) embryos have not been explored. Maize kernels can be cultured in vitro allowing experimental manipulation of environmental factors during seed development. We used the in vitro kernel culture system to investigate the response of developing maize seeds, which normally develop without exposure to light, to controlled light quality. Kernels grown under red light accumulated more dry weight than those grown in darkness, whereas kernels grown under blue light accumulated less. Reciprocal color shift experiments showed that light quality during the first week in culture had more influence on kernel weight than during the subsequent three weeks in culture. Soluble sugars were higher in both light treatments than in darkness. Blue-grown kernels had higher amino acid and lower lipid levels than red-or dark-grown kernels. Embryo morphology was markedly affected by red light, under which the upper shoot axis was longer than under blue light or in darkness. Embryo morphology was influenced by light quality during the later stages of development rather than the first week. We suggest, based on these results, that gene expression in the embryo and endosperm of developing maize seeds is sensitive to light quality, and the mechanism and time dependence of this effect warrant further study. In vitro maize kernel culture affords a convenient system for such light quality experiments.
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  • 93
    ISSN: 1573-5060
    Keywords: maize ; climatic factor ; genotype x environment interaction ; root trait ; statistical model ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The efficiency of genotopic and climatic characteristics in accounting for the interaction between genotypes and environments has been assessed in a three-year trial involving a set of genotypes presenting a range of root morphology characteristics (number and size). Climatic information on rainfall and temperature was recorded during the experiments, together with extra data on the growth and development of the genotypes. Their effects have been tested in factorial regression models. Climatic covariates were very powerful in accounting for the genotype by year interaction as well as the year main effect alone. For the number of adventitious roots on internode 7, the main effect of year could be described as a linear function of the average temperature and precipitation that occurred during the period of root initiation and growth. For internode 6, no clear conclusion was possible. For the root traits studied, 74 to 98% of the interaction could be explained by one climatic covariate. The regression coefficients can be considered as measures of genotypic stability. The genotypic covariates describing aerial development performed rather poorly, compared with environmental ones, even though the physiological and functional relationships between root and shoot are well known. Neither genotype main effect nor genotype by year interaction could be described sufficiently by factorial regression. Still, the genotypic covariates performing best clearly differed between root counts and size. Also the best genotypic covariates differed for main effect and interaction.
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  • 94
    ISSN: 1573-5060
    Keywords: germplasm registration ; maize ; Zea mays ; Isozyme ; markers ; morphological traits ; morphological variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Genetic markers (isozymes), in addition to the traits recommended by the UPOV convention, have been used in France since 1989 to characterize the maize inbred lines submitted to registration. In the years 1989. 1990 and 1991, a total of 974 inbreds has been described according to this procedure. Relationships between genetic markers were investigated and underlined the occurrence of linkage disequilibria within the tested germplasm. These disequilibria appeared to depend strongly on the breeding history of the germplasm. In some cases, these disequilibria could be related to a major progenitor (foundation effect). Relationships between genetic markers and quantitative traits were also pointed out. High coefficients of determination (up to 60%) were in some cases observed at the within group level, and also appeared to be very dependent on the major progenitors of the group of interest. The consequences of these results for breeding and distinctiveness studies are discussed.
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  • 95
    ISSN: 1573-5060
    Keywords: diallel cross ; qualitative interactions ; recurrent selection ; stability analysis ; maize ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A 10×10 diallel cross experiment involving white modified opaque-2 maize inbred lines was grown at four sites. A stability analysis, based on both a combining ability and a heterotic pattern model, was developed. The stability analysis provided valuable information on the genotype x environment interaction properties of the 10 inbred lines. The Gail & Simon (1985) test for qualitative interactions provided a means of determining the nature of these interactions. The inbred, SO507W(M), is shown to have the best potential for use in a hybrid breeding programme, in terms of having the highest weighted general combining ability and line heterosis, and the best general adaptability to all four sites. The single cross, SO713W(P) x PO558W(F), has been identified as one of the genotypes to be used in a recurrent selection programme that favours specific combining ability.
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  • 96
    ISSN: 1573-5060
    Keywords: genetic control ; maize streak virus ; major genes ; minor genes ; resistance ; Zea mays ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary S1 to S5 inbred lines, derived from a maize population bred for its overall resistance to three tropical viruses, were screened for resistance to maize streak virus (MSV) by artificial plant infection using viruliferous leafhoppers. Symptoms were rated and intra-line frequency distributions studied for all pedigree inbred lines. Mortality due to MSV was very low among these inbreds. Symptoms appeared later, developed slower and were less severe than in the susceptible control hybrid. Results of a study of 500 S1 and 93 S2 lines suggested that resistance is under genetic control via a system involving loci with major genes (with dominance for resistance) controlling high to complete resistance, associated with a genetic system involving loci with minor genes controlling partial resistance. Lines expressing complete resistance to MSV were developed from 5 cycles of inbreeding and selection. The relevance of such complete and partial resistance is discussed.
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  • 97
    ISSN: 1573-5060
    Keywords: glyphosate ; herbicide tolerance ; non-target effects ; somaclonal variation ; Zea mays ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The progeny of 104 regenerated maize plants were screened for tolerance to the safe broad-spectrum herbicide glyphosate during seed germination and early growth. Seven somaclones showed varying degrees of resistance to the application of the herbicide at 1.2 mM (0.1 kg a.i. in 400 1 ha-1 of water). Plants capable of a normal growth following treatment with 2.4 mM (0.2 kg ha-1) glyphosate at the three leaf stage were selfed, and their progeny analyzed. A family able to tolerate the exposure to glyphosate at 2.4 mM was isolated and shown to maintain a photosynthetic rate comparable with control after the application of the herbicide. The selfed progeny of the tolerant somaclone was characterized as to the properties of two targets of glyphosate, the shikimate pathway enzymes 5-enol-pyruvyl-shikimate-3-phosphate (EPSP) synthase and 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase. In vitro tests ruled out the possibility that the tolerance was due to altered forms of these enzymes. Families showed significant variability with regard to EPSP and DAHP synthase levels, measured at different stages during seedling growth; however, not even these traits were correlated with in vivo response to glyphosate. The possible role of other physiological processes in determining the increased tolerance to the herbicide is discussed.
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  • 98
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    Euphytica 82 (1995), S. 175-184 
    ISSN: 1573-5060
    Keywords: digestibility ; feeding value ; forage maize ; selection ; combining ability ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Variations in feeding value characteristics were studied on a factorial mating design, within a multilocal network. The in vitro digestibility of dry matter (IVDDM), neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), starch, soluble carbohydrate, protein and ash contents were evaluated on whole plant samples. The in vitro digestibility of the non-starch and non-soluble carbohydrate plant part (IVDNSC) was also computed from near infra-red reflectance spectroscopy estimations of IVDDM, starch and soluble carbohydrate contents. Variations due to general combining ability (GCA) were more important, for all traits, than variations due to specific combining ability (SCA). Variations due to the GCA × environment interaction were also sizeable and were partly explained by the earliness of the lines. IVDDM was primarily influenced by the relative proportions of cell walls and starch in the whole plant, which were particularly susceptible to the development stage of the plants, at harvest. When adjusted for earliness, there was generally no significant relation between the line per se value and GCA performance in the biochemical constituent contents of the plant. On the contrary, IVDNSC seemed to be a feeding value trait worth evaluating at the inbred line level. It was also almost independent of the ripening stage and could be easily computed from whole plant samples. IVDNSC, with a simultaneous control of starch content, might be a good selection criterion for evaluating and improving the nutritive value of forage maize.
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  • 99
    ISSN: 1573-5060
    Keywords: Zea mays ; insect vectors ; resistance ; maize ; maize streak virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Settling, probing and oviposition behaviour ofCicadulina mbila, the main vector of maize streak virus (MSV) disease were studied on four maize genotypes, 100MSR, HASR, Reunion and H512. Of the four, only H512 was completely susceptible to MSV while the other three genotypes were all known to have some resistance to MSV. Generally,C. mbila settled in higher numbers on the MSV susceptible genotype compared to the three virus resistant ones. However, the number of probing marks left on H512 and 100MSR were similar while more marks were made on Reunion and HASR in increasing order. The oviposition patterns were similar to those observed for probing behaviour. The results suggest the existence of potentially useful resistance mechanisms against the vector. The possible implications of these results are discussed.
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  • 100
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    Euphytica 84 (1995), S. 57-65 
    ISSN: 1573-5060
    Keywords: bush bean ; Phaseolus vulgaris ; intercropping ; maize ; sole cropping ; Zea mays ; quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The intereropping of common bean (Phaseolus vulgaris L.) and maize (Zea mays L.) is a common practice in the Northwest of the Iberian Peninsula. In this experiment, 25 populations of bush bean were evaluated for nutritional and culinary traits of dry seed and immature pod in sole crop and in intercrop in two years and in two locations, in order to determine whether selection of bush bean in sole crop can result in genetic progress for intercrop. There were no differences in the population means between cropping systems. Significant differences were detected among populations for all traits tudied except starch content, crude ash, water absorption and coat proportion. Population x environment interaction was significant for pod thickness. Bean populations did not interact with cropping systems for any trait, although there were differences between cropping systems for crude protein. These results suggest that a sole crop system provides sufficient information to select bean populations efficiently for the bush bean-maize intercropping system, although the advanced generations of the breeding program should be tested for quantitative traits such as protein content in the appropriate cropping system (intercropping) to know the competitive ability with the associated species. This would permit to choose bush bean populations which complement more efficiently and would be less competitive with the maize population.
    Type of Medium: Electronic Resource
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