Abstract
The bioavailability of heavy metals (Cd, Zn, Pb, Cu) and the abundance of arbuscular mycorrhiza (AM) were studied in two agricultural fields close to a Pb-Zn smelter and three fields outside the pollution zone all cultivated with maize (Zea mays L.). Metal extractability with ethylenediaminetetraacetic acid (EDTA)-NH4OAc and Ca(NO3)2, plant metal uptake, and mycorrhizal parameters (spore number, root colonization) were assessed at two growth stages (six-leaf and maturity). Despite regular liming, the availability of Cd, Zn, and Pb was markedly higher in the two metal-polluted fields than in the three uncontaminated fields. However, the AM abundance was not correlated with metal availability. Root colonization and spore numbers in the metal polluted fields were relatively high, though at plant maturity the former was significantly lower than in one of the uncontaminated fields. The very low AM abundance in the two other unpolluted fields was related to other factors, particular soil and plant P status and soil pH. AM root colonization did not substantially prevent plant metal accumulation, since the metal concentrations in maize grown on the polluted fields strongly exceeded normal values, and for Cd and Pb reached the limits of toxicity for animal feed.
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References
Angle JS, Heckman JR (1986) Effect of soil pH and sewage sludge on VA mycorrhizal infection of soybeans. Plant Soil 93:437–441
Commission of the European Community (1986) Council Directive on the protection of the environment and in particular of the soil, when sewage sludge is used in agriculture. CEC, Brussels, L 181:6–12
Dalenberg JW, Van Driel W (1990) Contribution of atmospheric deposition to heavy-metal concentrations in field crops. Neth J Agric Sci 38:369–379
Direction Régional de l'Industrie, de la Recherche et de l'Environment (1992) La qualité de l'air dans la région Nord/Pasde-Calais en 1991. DRIRE, Division Environment Industriel, Douai
Gildon A, Tinker PB (1983) Interactions of vesicular-arbuscular mycorrhizal infection and heavy metals in plants. I. The effect of heavy metals on the development of vesicular arbuscular mycorrhizas. New Phytol 95:247–261
Godin P (1986) Modèles pour une évaluation de la pollution des sols. Thèse Dr-Ing, Institut Nationale de la Recherche Agricole (INRA)/Paris-Grignon
Hepper CM, O'Shea J (1984) Vesicular-arbuscular mycorrhizal infection in lettuce (Lactuca sativa) in relation to calcium supply. Plant Soil 82:61–68
Hepper CM, Smith GA (1976) Observations on the germination of Endogone spores. Trans Br Mycol Soc 66:189–194
Hinesly TD, Jones RL, Ziegler EL, Tyler JJ (1977) Effects of annual and accumulative applications of sewage sludge on assimilation of zinc and cadmium by corn (Zea mays L.). Environ Sci Technol 11:182–188
Ietswaart JH, Griffioen WAJ, Ernst WHO (1992) Seasonality of VAM infection in three populations of Agrostis capillaris (Gramineae) on soil with or without heavy metal enrichment. Plant Soil 139:67–73
Jarvis SC, Jones LHP, Hopper MJ (1976) Cadmium uptake from solution by plants and its transport from roots to shoots. Plant Soil 44:179–191
Killham K, Firestone MK (1983) Vesicular arbuscular mycorrhizal mediation of grass response to acidic and heavy metal deposition. Plant Soil 72:39–48
Koomen I, McGrath SP, Giller I (1990) Mycorrhizal infection of clover is delayed in soils contaminated with heavy metals from past sewage sludge applications. Soil Biol Biochem 22:871–873
Koske RE, Gemma JN (1989) A modified procedure for staining roots to detect VA mycorrhizas. Mycol Res 92:486–505
Koske RE, Tessier B (1983) A convenient, permanent slide mounting medium. Mycol Soc Am Newslett 34:59
Leyval C, Berthelin J, Schontz D, Weissenhorn I, Morel JL (1991) Influence of endomycorrhizas on maize uptake of Pb, Cu, Zn and Cd applied as mineral salts or sewage sludges. In: Farmer JG (ed) Heavy metals in the environment. CEP Consultants Ltd, Edinburgh, pp 204–207
McGrath SP, Brookes PC, Giller KE (1988) Effects of potentially toxic elements in soil derived from past applications of sewage sludge on nitrogen fixation by Trifolium repens L. Soil Biol Biochem 20:415–424
Mench M, Vangronsveld J, Didier V, Clijsters H (1994) Evaluation of metal mobility, plant availability and immobilization by chemical agents in a limed silty soil. Environ Pollut 86:279–286
Menge JA, Steirle D, Bagyaraj DJ, Johnson ELV, Leonard RT (1978) Phosphorus concentrations in plants responsible for inhibition of mycorrhizal infection. New Phytol 80:575–578
Raju PS, Clark RB, Ellis JR, Maranville JW (1988) Effects of VA mycorrhizae on mineral uptake in sorghum genotypes grown on acid soil. Commun Soil Sci Plant Anal 19:909–918
Read DJ, Koucheki HK, Hodgson J (1976) Vesicular-arbuscular mycorrhiza in natural vegetation systems. I. Occurrence of infection. New Phytol 77:641–653
Sauerbeck D (1982) Welche Schwermetallgehalte in Pflanzen dürfen nicht überschritten werden, um Wachstumsbeeinträchtigungen zu vermeiden? Landwirtschaftl Forsch Sonderheft 39:105–129
Sauerbeck DR, Styperek P (1985) Significance of ion species and concentration for the determination of “available” cadmium in soil. FAO Eur Cooperative Network on Trace Elements Newslett 4:21–25
Stoeppler M (1991) Cadmium. In: Merian E (ed) Metals and their compounds in the environment. VCH, Weinheim, pp 803–851
Tinker PB, Gildon A (1983) Mycorrhizal fungi and ion uptake. In: Robb DA, Pierpoint SW (eds) Metals and micronutrients: uptake and utilization of metals by plants. Academic Press, London, pp 21–32
Trouvelot A, Kough JL, Gianinazzi-Pearson V (1986) Mesure du taux de mycorhization VA d'un système radiculaire. Recherche de méthodes d'estimation ayant une signification fonctionnelle. In: Gianinazzi-Pearson V, Gianinazzi S (eds) Aspects physiologiques et génétiques des mycorhizes. 1er Symp Eur sur les Mycorhizes. INRA, Paris, pp 217–221
Walker C, Mize CW, McNabb HS Jr (1982) Populations of endogenaceous fungi at two locations in central Iowa. Can J Bot 60:2518–2529
Wang GM, Stribley DP, Tinker PB, Walker C (1985) Soil pH and vesicular-arbuscular mycorrhizas. In: Fitter AH, Atkinson D, Read DJ, Usher MB (eds) Ecological interactions in soil: plants, microbes and animals. Blackwell, Oxford, pp 219–224
Weissenhorn I, Leyval C, Berthelin J (1993) Cd-tolerant arbuscular mycorrhizal (AM) fungi from heavy-metal polluted soils. Plant Soil 157:247–256
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Weissenhorn, I., Leyval, C. & Berthelin, J. Bioavailability of heavy metals and abundance of arbuscular mycorrhiza in a soil polluted by atmospheric deposition from a smelter. Biol Fertil Soils 19, 22–28 (1995). https://doi.org/10.1007/BF00336342
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DOI: https://doi.org/10.1007/BF00336342