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  • Lepidoptera  (383)
  • Springer  (383)
  • American Institute of Physics (AIP)
  • Elsevier
  • Institute of Physics
  • National Academy of Sciences
  • 2010-2014
  • 2000-2004  (26)
  • 1990-1994  (357)
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  • 1
    ISSN: 1570-7458
    Keywords: Lepidoptera ; Tortricidae ; reproduction ; tebufenozide ; methoxyfenozide ; ecdysone agonist ; Argyrotaenia velutinana ; Choristoneura rosaceana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects on the fecundity and fertility of redbanded leafroller, Argyrotaenia velutinana (Walker), and obliquebanded leafroller,Choristoneura rosaceana (Harris), exposed as adults to surfaces treated with the ecdysone agonists tebufenozide (RH-5992) and methoxyfenozide (RH-2485) were examined. The first part of the study consisted of recently emerged moths being exposed to treated surfaces continuously throughout their lives (including mating and oviposition). Continuous exposure to tebufenozide- or methoxyfenozide-treated surfaces significantly reduced the mean number of eggs laid and the percent of eggs that hatched in both species. The second part of the study involved exposure of recently emerged virgin moths (by sex) to treated surfaces for 24 h, after which, the exposed moths were paired with a nontreated partner to mate and oviposit on nontreated surfaces. In this experiment, for A. velutinana, significant reductions in fecundity occurred only when the female was exposed to methoxyfenozide-treated surfaces. Significant reductions in A. velutinana egg fertility occurred with both male and female exposure in the methoxyfenozide treatments and only female exposure in the tebufenozide treatments. For C. rosaceana, significant reductions in fecundity occurred with both male and female exposure in the tebufenozide and methoxyfenozide treatments. Significant reductions in C. rosaceana egg fertility occurred with both male and female exposure in the tebufenozide treatments and only with female exposure in the methoxyfenozide treatments.
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  • 2
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    Entomologia experimentalis et applicata 95 (2000), S. 217-227 
    ISSN: 1570-7458
    Keywords: antifeedant ; drimane ; mechanism of action ; sensory response ; toxicity ; habituation ; behavioural observation ; structure-activity relationship ; Pieris brassicae ; Lepidoptera ; Pieridae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We tested 11 analogous synthetic drimane antifeedant compounds for their feeding inhibiting effects on larvae of the large white butterfly Pieris brassicae L. (Lepidoptera: Pieridae) in no-choice tests on the host plant Brassica oleracea L. Furthermore, we observed larval feeding behaviour in no-choice tests to analyze temporal effects of five drimanes. The results show that the five analogous antifeedants differentially influence feeding behaviour and locomotion activity. Warburganal and polygodial are most likely sensory mediated antifeedants. Habituation to these compounds occurs soon after the onset of the tests (i.e., within 0.5–1.5 h). Compound 5 and confertifolin are probably not direct, sensory mediated antifeedants. After 0.5–1.5 h of exposure, these compounds inhibit not only feeding, but also locomotion behaviour, indicating postingestive, toxic effects. Isodrimenin inhibits feeding from the onset of the test and is probably a sensory mediated antifeedant. No habituation occurs to this compound, indicating that isodrimenin is either a very strong antifeedant or that it additionally has postingestive, toxic effects. Topical application of the drimanes on the larval cuticle revealed feeding inhibiting effects, but these could not be related to the occurrence of postingestive feeding inhibiting effects, indicating that this method is inappropriate to show possible postingestive effects of drimanes in P. brassicae. In conclusion, the behavioural observations performed in this research indicate that analogous drimanes inhibit feeding by P. brassicae larvae through multiple mechanisms of action. The results show that, when developing a structure activity relationship (SAR) for a series of antifeedants, it is important to distinguish the mode of action which underlies inhibition of feeding.
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  • 3
    ISSN: 1570-7458
    Keywords: Cnaphalocrocis medinalis ; rice leaffolder ; Lepidoptera ; Pyralidae ; sex pheromone ; geographical variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sex pheromone components of the Japanese rice leaffolder moth, Cnaphalocrocis medinalis Guenée (Lepidoptera: Pyralidae) were identified from ovipositor extracts of virgin females as (Z)-11-octadecenal, (Z)-13-octadecenal, (Z)-11-octadecen-1-ol and (Z)-13-octadecen-1-ol at a ratio of 11:100:24:36 by GC-EAD, GC, GC-MS. The total amount was estimated to be ca.0.9 ng/female. Field bioassays in Kagoshima, Japan, showed that the two aldehydes are essential for male attraction and the alcohols may have a synergistic effect on the aldehydes. A rubber septum containing 0.9 mg of the four components at the natural ratio was shown to be an effective lure for monitoring this pest in Japan. The above four components are quite different from the sex pheromone components reported previously for the same species of either Philippine or Indian origin; components were shown to be (Z)-11-hexadecenyl acetate and (Z)-13-octadecenyl acetate at a ratio of 98:2 in the Philippine blend and 1:10 in the Indian blend. Furthermore, in the field tests in Japan, neither the Philippine blend nor the Indian blend showed any attractive activity, while the Japanese blend attracted significant numbers of male moths. These results suggest that there are remarkable geographical variations in the sex pheromone composition of this species or there are several distinct species using different sex pheromone blends.
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  • 4
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    Entomologia experimentalis et applicata 97 (2000), S. 137-147 
    ISSN: 1570-7458
    Keywords: Etiella zinckenella ; Etiella hobsoni ; Lepidoptera ; Pyralidae ; soybean ; development ; reproduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Experiments conducted in West Java, Indonesia investigated the developmental biology and reproductive behavior of two sympatric soybean pod borers, Etiella zinckenella Treitschke and E. hobsoni Butler (Lepidoptera: Pyralidae). It was determined that: (1) significant interspecific differences occurred between the egg, larval, pupal and total egg-to-adult developmental periods of laboratory raised E. zinckenella and E. hobsoni; (2) the pre-pupal and total egg-to-adult development periods of female E. zinckenella were significantly shorter than for males; (3) the longevity of virgin female E. zinckenella was significantly longer than that of virgin males, or virgin male and female E. hobsoni; (4) interspecific differences occurred in the female: male sex-ratios of laboratory raised adults; (5) peak mating for both species occurred on the second night after eclosion; (6) interspecific differences occurred in the temporal distribution of calling and mating behaviors; (7) repeated mating was observed for both species at a very low frequency; (8) interspecific mating did not occur; (9) female E. zinckenella were significantly more fecund than E. hobsoni; (10) the duration in copulo of E. zinckenella was significantly longer than that of E. hobsoni; and (11) wingtraps baited with virgin females caught only conspecific males, and reduced numbers of males were captured in traps simultaneously baited with virgin females of both species. This study demonstrates distinct biological differences and reproductive isolation between the two Etiella spp.
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  • 5
    ISSN: 1570-7458
    Keywords: calling behaviour ; periodicity ; sex pheromone ; attraction inhibitor ; attraction synergist ; field tests ; Lepidoptera ; Gracillariidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Females of the leaf miner moth Phyllonorycter acerifoliella (Z.) [=Ph. sylvella (Hw.)] and Ph. heegerella (Z.) (Lepidoptera: Gracillariidae: Lithocolletinae) release their sex pheromone at the beginning of photophase. The periodicity of the `calling' behaviour of Ph. acerifoliella females was established. Three compounds from calling virgin Ph. heegerella females were collected by the Solid Phase Micro Extraction (SPME) technique and identified as (Z)-8-tetradecenyl acetate (Z8-14:OAc), tetradecyl acetate (14:OAc) and (Z)-8-tetradecenol (Z8-14:OH) in the ratio (88±3):(2±0.6):(10±5) by capillary gas chromatography and mass spectrometry. Field trapping experiments demonstrated that the first two compounds are important for the attraction of conspecific males. Z8-14:OAc was found to be attractive when tested separately, while 14:OAc acted as synergist. The attractivity of the three component blend was reduced by 10% admixture of either (E)-10-dodecenyl acetate (E10-12:OAc) or (Z)-10-tetradecenyl acetate (Z10-14:OAc). Field tests of Z10-, Z8- and E10-14:OAc, identified from Ph. acerifoliella females, demonstrated that the first two compounds were essential for the attraction of conspecific males; so both are sex pheromone components. The attractivity of the three component blend of Z10- Z8- and E10-14:OAc was reduced by 10% admixture of (E)-10-dodecenol (E10-12:OH). The following four semiochemical compounds, Z8-14:OAc, Z8-14:OH, E10-14:OAc and 14:OAc, identified from phyllonoryctid females, as well as two sex attraction antagonists for Ph. acerifoliella and Ph. heegerella males, E10-12:OAc and Z10-14:OAc, are new for the family Gracillariidae. The results of field trapping experiments revealed mechanisms ensuring the specificity of the chemocommunication systems in Ph. acerifoliella, Ph. heegerella and Ph. ulmifoliella (Hb.) moths.
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  • 6
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    Entomologia experimentalis et applicata 97 (2000), S. 339-346 
    ISSN: 1570-7458
    Keywords: crygenes ; PCR ; Lepidoptera ; Spodoptera ; insecticidal activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thirty-five strains of the entomopathogenic bacterium Bacillus thuringiensisactive on Spodoptera exigua, were characterized by means of serological identification and determination of crygene contents by PCR. The insecticidal activity of these 35 strains was further confirmed against S. exiguaand tested against two other species of the same genus: S. littoralisand S. frugiperda. The results indicate that serovars aizawai, thuringiensis, and kurstakiwere the most frequent within S. exigua-active strains and that serovar aizawaihad the highest number of strains exhibiting toxicity against the three species bioassayed. The presence in crygenes as determined by PCR suggests a non random distribution of some crygenes among serovars. Genes cry1C, cry1D, and cry1E, which are known to code for proteins toxic against Spodopteraspecies, were very common within S. exigua-active strains, specially in those belonging to serovar aizawai. However, some strains harbouring one or more of these genes were not toxic to S. littoralisor S. frugiperda; and some strains lacking all of the Spodoptera-active genes were found to be toxic to all three species. This suggests differences in the expression levels among strains bearing toxic genes and the involvement of other genes toxic to Spodopteraspecies. Since strains sharing the same crygenes exhibited different host ranges, the results indicate the need to perform toxicity bioassays in addition to other tests (serological identification and PCR) in order to determine the insecticidal activity of B. thuringiensisstrains.
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  • 7
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    Journal of insect behavior 13 (2000), S. 881-900 
    ISSN: 1572-8889
    Keywords: three-dimensional ; flight track recording ; orientation ; pheromones ; plant volatiles ; image analysis ; real time ; Lobesia botrana ; Lepidoptera ; Tortricidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A computer-controlled video system for real-time recording of insect flight in three dimensions is described. The flight paths of moths were recorded in a flight tunnel using two CCD cameras placed adjacent to each other at angles of 45 and 135° to the flight tunnel axis and separated by a distance of 120 cm. They were connected to two 28-level gray-scale frame grabbers via two external synchronizers. The two-dimensional coordinates of the flying insect were obtained from the two cameras at 40-ms intervals and transferred to host computer for processing and monitor for real-time display. Due to speed limitation in the image acquisition hardware, construction of the three-dimensional file was carried off-line. The flying insect was rendered as a dark spot in a bright background using a homogeneous light source. As the insect enters into the field of view of the two cameras, the light distribution changes, and the frame grabber detects only those variation in the light distribution which results from a flying insect. The target insect can be as small as 3 pixels and can be tracked in a stereoscopic field of view 60 cm long and 50 cm high. A method was developed that allowed for scalar scoring of various pheromone sources to assess their attractiveness using vector flight parameters. This method was applied successfully for optimization of pheromone blend of the grapevine moth, Lobesia botrana.
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  • 8
    ISSN: 1437-5613
    Keywords: Key words Herbivory ; Plant–herbivore interaction ; Lepidoptera ; Cruciferous plants ; Vegetation texture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Correlation between plant size and reproductive output may be modified by herbivory in accordance with host plant density and the presence of nonhost plants. To elucidate the effects of nonhost plant density and host plant density on the intensity of herbivory and reproductive output of the host plant in relation to plant size under natural conditions, we investigated the abundance of three lepidopteran insects, Plutella maculipennis, Anthocharis scolymus, and Pieris rapae the intensity of herbivory, and fruit set of their host plant, Turritis glabra (Cruciferae). To elucidate the effects of nonhost and host plant density, we selected four categories of plots under natural conditions: low density of nonhost and high density of host plants; low density of both nonhost and host plants; high density of both nonhost and host plants; and high density of nonhost and low density of host plants. The plant size indicated by stem diameter was a good predictor of the abundance of all herbivorous species. The effects of density of nonhost and host plants on the abundance of insects varied among species and stages of insects. As the abundance of insects affected the intensity of herbivory, herbivory was more apparent on larger host plants in plots with low density of both nonhost and host plants. Consequently, the correlation between plant size and the number of fruits disappeared in low plots with density of both nonhost and host plants. In this T. glabra– herbivorous insect system, the density of nonhost plants and host plants plays an important role in modifying the relationship between plants and herbivores under natural conditions.
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  • 9
    ISSN: 1437-5613
    Keywords: Key words Community patterns ; Lepidoptera ; Route order ; Species richness ; Species distribution ; Environmental disturbance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Kitahara and Fujii, in 1994, analyzed the butterfly communities along a gradient of human disturbance by applying the generalist/specialist concept. Butterfly species were classified into generalist or specialist species based on their voltinism (seasonal time dimension) and potential larval resource breadth (food dimension). The community structure and species composition showed the systematic changes along the gradient. To verify the generality of those trends, we monitored five grassland butterfly communities with varying degrees of human disturbance twice a month during 1985 by the line transect method at the foot of Mt. Fuji, central Japan, and analyzed their structure in a manner similar to that employed by Kitahara and Fujii. Most results were consistent with the patterns recognized by Kitahara and Fujii. The route (community) order based on increasing human disturbance was strongly and negatively correlated with butterfly species richness but with neither butterfly species diversity (H′) nor evenness (J′). Also, the degree of human disturbance was significantly and negatively correlated with the number of specialist species, but not with that of generalists, in a community. Butterfly species richness was more strongly correlated with the number of specialist species than with that of generalists. Our analyses also showed that the generalist species were distributed more widely over the communities than were the specialists. However, in contrast to the trend revealed by Kitahara and Fujii, there was no significant difference in the population densities and in the spatial population variability between the two species groups. As a whole, our analyses confirmed the consistency of most community patterns detected by Kitahara and Fujii. The causes of the inconsistencies in some patterns were thought to be mainly the present habitat conditions with a relatively short growing season at high altitudes.
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  • 10
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    Journal of insect conservation 4 (2000), S. 73-77 
    ISSN: 1572-9753
    Keywords: mapping ; database ; bias recording ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Nature of Science, Research, Systems of Higher Education, Museum Science
    Notes: Abstract We use data from the Mersey Valley zone (3×2 km area; N = 600 I ha squares) of the Greater Manchester butterfly atlas to investigate whether recorder visits are biased by access (viz. distance from recorder's home base) and by the locations of potential hot spots. In a multiple regression analysis, visits were found to correlate significantly both with distance from home base of the recorder and with the mean and maximum number of species found in squares. Sites close to the home base of the recorder were visited more frequently than those further afield and squares with more species were visited more frequently than those squares with fewer species. Visits were also made significantly more frequently to squares with greater numbers of butterfly resources (e.g. hostplants, nectar). Furthermore, recording is biased to and away from distinct land uses, which vary significantly in species richness. Reasons are given why these biases are to be expected at all scales. The message is that future distribution mapping should be based on rigorous sampling approaches.
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  • 11
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    Oecologia 122 (2000), S. 1-10 
    ISSN: 1432-1939
    Keywords: Key words Distribution ; Gregariousness ; Larval strategy ; Lepidoptera ; Thermoregulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Group-living in animal populations has many possible ecological and evolutionary explanations, including predator evasion and feeding facilitation. In insects, the thermal balance of solitary and gregarious larvae is likely to differ and may thus have important ecological consequences. The abilities of the larvae of four species of nettle-feeding nymphalid butterflies to thermoregulate were quantified in the field. Larval surface body temperatures of the gregarious Aglais urticae (small tortoiseshell) and Inachis io (peacock) and the solitary Polygonia c-album (comma) and Vanessa atalanta (red admiral) were measured for each instar, in both sunny and overcast conditions, over a seasonal range of temperatures. The results suggested two distinct larval thermal strategies. In the presence of direct sunlight, the exposed gregarious larvae of A. urticae and I. io regulated body temperatures at 32.5 and 31.5°C, respectively, while the temperatures of concealed larvae of P. c-album and V. atalanta were largely dependent on ambient temperatures. In the sun, the range of body temperatures recorded for A. urticae and I. io larvae was fairly narrow relative to ambient temperatures. This suggests a high degree of thermal control in these species. Modal body temperatures coincided with the temperature at which development rate is maximal. Regardless of whether changes in thermoregulation are a cause or consequence of the evolution of gregariousness, the combination of behavioural thermoregulation and gregariousness in larval insects has important implications for voltinism patterns and range extension (via increased development rates). Distributional responses of gregarious and solitary larvae to climatic warming may differ as a result of changes in cloud cover as well as changes in temperature.
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  • 12
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    Oecologia 122 (2000), S. 36-43 
    ISSN: 1432-1939
    Keywords: Key words Life history theory ; Longevity ; Ageing ; Mortality rate ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Life history theory predicts that organisms should only invest resources into intrinsic components of life span to the degree that it pays off in terms of reproductive success. Here, we investigate if the temporal distribution of mating opportunities may have influenced the evolution of intrinsic mortality rates in the butterfly Pararge aegeria (Satyrinae). In this species, females mate only once and the frequency of male mating opportunities depends on the temporal emergence pattern of virgin females. As expected, in a population from Madeira where females emerge continuously throughout the year, there was no sex difference in adult life span, while in a Swedish population with synchronised female emergence, males had significantly shorter life spans compared to females. A logistic mortality model provided the best fit to the observed change in age-specific mortality and all categories reached an asymptotic mortality rate of a similar magnitude. However, the Swedish males reached this mortality plateau more rapidly than the other categories. External mortality, due to water and food limitation, affected the pattern of sex-specific mortality but males from Sweden still had higher rates of mortality compared to all other categories. We argue that selection on male longevity is likely to be weaker in Sweden because under synchronised emergence, all females emerge and mate within a short period of time, after which male reproductive value will quickly approach zero. On Madeira, however, male reproductive value decrease more slowly with age since the probability of finding a receptive female is constant over the year.
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  • 13
    ISSN: 1573-1561
    Keywords: Insect pheromones ; pheromone emission ; aggressive chemical mimicry ; predation ; allomone ; Araneae ; Araneidae ; Lepidoptera ; Noctuidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The adult female bolas spider Mastophora hutchinsoni feeds exclusively on attracted males of a few moth species. This exclusivity and the behavior of the approaching moths suggest that the spider aggressively mimics the sex pheromones of its prey species. Males of the bristly cutworm, Lacinipolia renigera, are a major prey of this spider, accounting for about two thirds of the biomass of prey consumed. Female bristly cutworms produce a pheromone blend consisting of (Z)-9-tetradecenyl acetate (Z9–14 : Ac) and (Z,E)-9,12-tetradecenyl acetate (ZE-9,12–14 : Ac). To determine if M. hutchinsoni females mimic the sex pheromone components and blend ratio of L. renigera, we collected volatiles from hunting adult female spiders and analyzed them with gas chromatography–electroantennographic detection (GC-EAD) and gas chromatography–mass spectrometry (GC-MS). GC-EAD analysis of volatile collections, using a male bristly cutworm antenna as the detector and two capillary columns of different polarities, revealed the presence of peaks with retention times (R ts) identical to Z9–14 : Ac and ZE-9,12– 14 : Ac. The mass spectrum of a peak with R t of Z9–14 : Ac was identical to the mass spectrum of the synthetic equivalent. There was an insufficient quantity of the compound with R t of ZE-9,12–14 : Ac to get a full spectrum, but selective detection of ions at m/z 61 and 192 at the correct R t supported the identification. On average, the blend collected from spiders contained 54.8 ± 20.8 (SE) pg/min of Z9–14 : Ac and 2.5 ± 1.7 (SE) pg/min of ZE-9,12–14 : Ac. The latter, on average, comprised 2.6 ± 0.7% of the total, which is similar to the blend ratio emitted by bristly cutworm females. Our results indicate that the adult female M. hutchinsoni produces an allomone blend that mimics not only the composition, but also the blend ratio, of the sex pheromone of a major prey species.
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  • 14
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    Journal of chemical ecology 26 (2000), S. 1983-1990 
    ISSN: 1573-1561
    Keywords: Setora nitens ; Setothosea asigna ; nettle caterpillar ; Limacodidae ; Lepidoptera ; sex pheromone ; (Z)-9-dodecenal ; (Z)-9,11-dodecadienal ; (E)-9-dodecenal ; (E)-9,11-dodecadienal ; oil palm ; Elaeis guineensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Gas chromatographic–electroantennographic detection (GC-EAD) analyses of pheromone gland extracts of female nettle caterpillars, Setora nitens, revealed four compounds that consistently elicited responses from male moth antennae. Retention indices on three fused silia columns (DB-5, DB-23, and DB-210) of two EAD-active compounds were almost identical to those of (E)-9-dodecenal (E9–12 : Ald) and (E)-9,11-dodecadienal (E9,11–12 : Ald), two pheromone components previously identified in congeneric Setothosea asigna. However, comparative GC, GC-EAD, and GC-mass spectrometry of extracted S. nitens compounds and authentic standards revealed that the candidate pheromone components were (Z)-9-dodecenal (Z9–12 : Ald) and (Z)-9,11-dodecadienal (Z9,11–12 : Ald). The two other EAD-active compounds in pheromone gland extracts proved to be the corresponding alcohols to these aldehydes. In field-trapping experiments in Tawau, Malaysia, synthetic Z9–12 : Ald and Z9,11–12 : Ald at a 1 : 1 ratio, but not singly, attracted male S. nitens. Attractiveness of these two aldehydes could not be enhanced through the addition of their corresponding alcohols. Whether these differences in pheromone biology and chemistry between S. nitens and S. asigna are sufficient to prevent cross-attraction of heterospecific males or whether nonpheromonal mechanisms are required to maintain reproductive isolation is currently being studied.
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  • 15
    ISSN: 1432-0762
    Keywords: Key words Copulation duration ; Explosive mating system ; Ejaculate size ; Lepidoptera ; Mating history
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The mating system of the Australian lycaenid butterfly, Jalmenus evagoras, is highly unusual compared to most other Lepidoptera. Characteristics of this system, which has been termed an ’explosive mating strategy,’ include the formation of an intensely competitive mating aggregation of males, a highly male biased operational sex ratio, a lack of discrimination and mate choice by both sexes, a high variance in male mating success, and female monogamy. In this study, we tested the hypothesis that multiple mating by males imposes physiological costs resulting in smaller spermatophores, and that this results in a fitness cost to females. We found that male J. evagoras transferred only 2.2% of their eclosion weight during their first mating, consistent with the hypothesis that males of monandrous species produce a relatively small investment. The wet weight of the ejaculate declined by an average of 27% at the second mating and the dry weight by 29%, and an intermating interval of 5–9 days was needed for the ejaculate to return to the size at the first mating, regardless of male size or age. Wet ejaculate mass increased proportionally with male size, though dry mass was proportionally larger in smaller males. Ejaculate mass tended to increase with male age at both first and second matings. Female characteristics, in general, did not affect ejaculate mass, although the wet weight of the ejaculate was positively associated with female weight at the second mating. Copulation duration increased from 2.4 h to approximately 3 h at the second mating, and to over 4 h at the third and fourth matings. Fecundity was positively correlated with female size but not with mating history, copulation duration, or any other characteristics measured for either males or females. Female longevity declined significantly as the number of times the male partner had previously mated increased. We conclude that despite the small male investment in ejaculate, the costs of multiple mating may nonetheless be significant, as indicated by the reduction in ejaculate mass, an increase in copulation duration, and reduction in female lifespan with increasing mating number.
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  • 16
    ISSN: 1573-1561
    Keywords: Insecta ; tobacco hornworm ; Manduca sexta ; tobacco ; Nicotiana tabacum ; ozone ; weather fleck ; Lepidoptera ; Sphingidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Tobacco plants, Nicotiana tabacum were grown under different levels of ozone (O3) in open-top chambers. Ozone concentrations were established by charcoal filtration, which reduced O3 to approximately one-half ambient, or by the addition of O3 to unfiltered air to increase concentrations to approximately 1.4 or 1.7 times ambient O3. Survival of tobacco hornworm, Manduca sexta, larvae was increased when second instars were fed tobacco leaves grown in chambers with elevated levels of O3. Second instars also gained significantly more weight when they were fed for one week on plants exposed to elevated levels of O3 than when they were fed plants grown in charcoal-filtered air. Ozone-treated tobacco plants had higher levels of total nitrogen (primarily reduced nitrogen) and soluble carbohydrates (sugars), and lower levels of leaf-surface components, starch, nicotine, and rutin. Increased survival and growth response of hornworm larvae to elevated O3 levels in these experiments suggests that similar responses could occur in the southeastern US tobacco production areas where O3 levels can be high enough to injure tobacco plants.
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  • 17
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Tortricidae ; Enarmonia formosana ; cherry bark tortrix ; kairomone ; host selection ; foraging behavior ; plant volatiles ; nonhost volatiles ; repellency/inhibition ; oviposition deterrence ; hexanol ; benzyl alcohol ; nonanal ; decanal ; α-pinene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We tested the hypothesis that males and females of the cherry bark tortrix (CBT), Enarmonia formosana, antennally perceive and behaviorally respond to volatiles from nonhost plants. Volatiles from flowering cherry trees, Prunus serrulata Kwanzan, and from nonhost trees, including trembling aspen, Populus tremuloides, grand fir, Abies grandis, Norway spruce, Picea abies, and Scots pine, Pinus sylvestris, were captured on Porapak Q and extracts analyzed by coupled gas chromatographic–electroantennographic detection (GC-EAD). Compounds that elicited responses from male and female antennae were identified by coupled GC–mass spectrometry (MS) and by comparative GC-MS and GC-EAD with authentic standards. In field cage and field experiments, nonanal from aspen trees and (±)-α-pinene from coniferous trees were effective in reducing captures of male CBT in pheromone-baited traps and deterring oviposition by female CBT on cherry blocks. Recognition of nonhost volatiles, such as nonanal and α-pinene, may allow male and/or female CBT to avoid trees that provide inadequate concealment from predators during calling, resting, and copulation and that are unsuitable for oviposition and development of offspring. Nonhost volatiles may also be exploited for control of CBT by disrupting both mate- and host-finding.
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  • 18
    ISSN: 1573-1561
    Keywords: Field trapping ; pheromone ; Lepidoptera ; Gelechiidae ; Tuta absoluta ; South American tomato moth ; Scrobipalpuloides absoluta
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Field evaluations demonstrate that the addition of the minor pheromone component (3E,8Z)-tetradecadien-1-yl acetate to the major component (3E,8Z,11Z)-tetradecatrien-1-yl acetate does not significantly increase the trap catches of Tuta absoluta males in the field. The triene acetate itself is highly attractive, catching about 869 ± 255 males per trap in three consecutive nights. The addition of two isomers of the minor component (3E,8Z)-tetradecadien-1-yl acetate, (3E,11Z)-tetradecadienyl-yl acetate and (8Z,11Z)-tetradecadien-1-yl acetate, to the major component (3E,8Z,11Z)-tetradecatrien-1-yl acetate also did not significantly alter the number of the males caught in the traps.
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  • 19
    ISSN: 1573-1561
    Keywords: Scoliopteryx libatrix ; Noctuidae ; Lepidoptera ; sex pheromone ; methylalkene ; (6Z13)-methylheneicosene ; electroantennogram ; field test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The major component of the female-produced sex pheromone of Scoliopteryx libatrix has been characterized by chemical analysis, synthesis, electrophysiological studies and field tests as (6Z,13)-methylheneicosene, probably the 13S-isomer. This is the first example of a branched chain alkene as a sex pheromone in the Noctuidae and is markedly different from the pheromones of other members of the family. The systematic position of S. libatrix, belonging to a monotypic genus of a one-member subfamily within the Noctuidae, may reflect the unusual structure of the sex pheromone.
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  • 20
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Limacodidae ; Darna trima ; Darna bradleyi ; Setothosea asigna ; Setora nitens ; nettle caterpillars ; oil palm ; sex pheromone ; 2-methylbutyl (E)-7,9-decadienoate ; (E)-2-hexenyl (E)-7,9-decadienoate ; methyl (E)-7,9-decadienoate ; isobutyl (E)-7,9-decadienoate ; (E)-9dodecenal ; (E)-9,11-dodecadienal ; (Z)-9-dodecenal ; (Z)-9,11-dodecadienal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract This study was undertaken to identify sex pheromone components of nettle caterpillars Darna trima and Darna bradleyi (Lepidoptera: Limacodidae) whose larvae defoliate oil palm, Elaeis guineensis, in southeast Asia. Coupled gas chromatographic–electroantennographic detection (GCEAD) analyses of pheromone gland extracts revealed two antennally active compounds produced by female D. trima and two by female D. bradleyi. Molecular structures of these candidate pheromone components were identified by electron-impact and chemical-ionization mass spectrometry; retention-index calculations on DB-5, DB-23, and DB-210 columns; microanalytical treatments, as well as syntheses of "auxilliary" compounds that facilitated identification of the compounds. The compounds from D. trima were 2-methylbutyl (E)-7,9-decadienoate (A) and (E)-2-hexenyl (E)-7,9decadienoate (B); from D. bradleyi we identified methyl (E)-7,9-decadienoate (C), and isobutyl (E)-7,9-decadienoate (D). In field experiments in Malaysia, (S)-2-methylbutyl (E)-7,9-decadienoate (SA) in combination with B proved to be essential and synergistic pheromone components for attraction of male D. trima. (R)-2-Methylbutyl (E)-7,9-decadienoate (RA) had no behavioral activity. Compound D singly attracted male D. bradleyi, but addition of C to D at a 1 : 10 ratio significantly enhanced attractiveness of the bait. Synthetic pheromone blends were more effective trap baits than unmated female moths and could be developed for monitoring populations of D. trima and D. bradleyi in Asian oil palm plantations.
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  • 21
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    Biodiversity and conservation 9 (2000), S. 479-486 
    ISSN: 1572-9710
    Keywords: biodiversity ; butterfly ; Ghana ; insect ; lagoon ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A variety of collection methods were used to inventory the insect diversity of the Muni-Pomadze Ramsar site along the Ghana coastline. A total of 75 butterfly species in five families were collected and identified. Twenty-six percent of the butterfly species were open country species. The butterflies were all typical of the coastal zone and no endangered or narrowly endemic species were recorded. The Muni-Pomadze site was also rich in other insect species (67 insect species belonging to 15 orders) as a result of the diverse terrestrial habitats surrounding the lagoon. Butterfly species composition changed with habitat indicating a fine-grained response by the butterfly communities to habitat changes. Data from long-term monitoring of butterfly communities at Muni-Pomadze could prove useful as indicators of habitat quality.
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  • 22
    ISSN: 1573-1561
    Keywords: Megalophanes viciella ; Psychidae ; Lepidoptera ; bagworm ; sex pheromone ; 1-methylethyl octanoate ; 2-propyl octanoate ; field tests
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Extracts obtained by rinsing the thorax and extracts from the tergal sex pheromone gland of females of the bagworm moth, Megalophanes viciella, as well as headspace samples from live animals, were analyzed to reveal the chemical structure of the pheromone. As a result, 1-methylethyl octanoate was established as a main sex pheromone component of this species. This was further confirmed by numerous catches of conspecific males in traps baited with this compound in Bulgaria and Roumania.
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  • 23
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    Journal of chemical ecology 26 (2000), S. 2549-2555 
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Carposinidae ; (Z)-12–nonadecen-9–one ; chemical identification ; trapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The female-produced sex pheromone of the New Zealand raspberry budmoth, Heterocrossa rubophaga, was investigated. Gas chromatographic–mass spectrometric analyses revealed the ketone, (Z)-12–nonadecen9–one (Z12–19–9–one). This compound had previously been found in extracts of the sex pheromone gland of the only other carposine moth for which a sex pheromone has been identified, Carposina niponensis, although its effect on the behavior of C. niponensis males had not been established. Field trapping trials in berry fruit gardens showed this compound to elicit high catches of male H. rubophaga, with the catch appearing to plateau (and perhaps decrease) above a dosage of 300 μg on a red rubber septum. In an analysis of an extract of female H. rubophaga sex pheromone glands, there was a suggestion that the homologous (Z)-7–eicosen-11–one, the known sex pheromone component of C. niponensis, was also present. However this could not be established unequivocally and, in a field trial, addition of a small amount of this compound to Z12–19–9–one resulted in no significant increase in trap catch relative to traps baited with Z12–19–9–one alone.
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  • 24
    ISSN: 1573-1561
    Keywords: Female sex pheromones ; Lepidoptera ; Geometridae ; Ennominae ; epoxynonadecene ; epoxynonadecadiene ; chiral epoxide ; chiral HPLC ; dimethyl disulfide adduct
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Biston robustum Butler, a polyphagous defoliator, multiplied on Hachijo-jima Island in 1997–1998. Based on GC-MS data of authentic standards, an analysis of a pheromone gland extract of the females indicated that it included (Z,Z)-6,9–nonadecadiene (I), (Z,Z,Z)-3,6,9–nonadecatriene (II), cis-(Z)-6,7–epoxy-9–nonadecene (III), and cis-(Z,Z)-6,7–epoxy-3,9–nonadecadiene (IV) in a ratio of 13 : 2 : 70 : 15. The structure of III was confirmed by a GC-MS analysis of another extract treated with dimethyl disulfide (DMDS). This epoxymonoene was successfully converted into the corresponding DMDS adduct that showed diagnostic ions fragmented at an epoxy ring and at thiomethoxy groups reflecting the position of an original double bond. Furthermore, the 6S,7R configuration was assigned for the epoxy ring of III by chiral HPLC analysis. Field examination of synthetic lures revealed that the two epoxy compounds (III and IV) with the 6S,7R configuration were essential components and that the two unsaturated hydrocarbons (I and II) showed a synergistic effect on male attraction.
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  • 25
    ISSN: 1573-8248
    Keywords: biological control ; blueberry ; egg parasitoid ; inundative release ; leafroller ; Lepidoptera ; Tortricidae ; Hymenoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Inundative releases of Trichogramma minutum Riley were made in a commercialblueberry field in British Columbia, Canada, todetermine the extent of dispersal of adult wasps, andto determine whether complete parasitism of eggswithin egg masses of the target host [oblique-bandedleafroller (OBLR), Choristoneura rosaceana(Harris)] occurred. Three weekly releases of waspswere made from a single release point in the center ofeach field during the flight of first generation OBLRadults. Sticky trap, sentinel egg mass andtarget-host egg mass data all indicated only a limitedrange of dispersal of T. minutum within thefield. In addition, only partial parasitism of eggmasses of the target host occurred even in closeproximity to the release point. We discuss theimportance of determining the response of Trichogramma females to previously-parasitized eggswhen using Trichogramma wasps for biologicalcontrol of lepidopterous species that oviposit largeegg masses.
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  • 26
    ISSN: 1573-8248
    Keywords: bird predation ; forest ; interference ; Italy ; Lepidoptera ; pine processionary caterpillar ; parasitism,/kwd〉 ; Pinus ; Thaumetopoeidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The hoopoe (Upupa epops L.) is an insectivorousbird often preying on larvae and pupae of the pineprocessionary caterpillar (Thaumetopoeapityocampa Denis & Schiffermüller), the mostimportant defoliator of pines in Southern Europe andNorthern Africa. The first half of the breeding seasonof this migratory bird coincides with the pupal stageof the insect. Bird predation of pupae was quantifiedin two years in a Pinus nigra stand of northernItaly by periodic counting of the empty cocoons lefton the soil surface after extraction. The predationrate, estimated on 20 pupation sites representing thewhole stand, was 74.1% in 1993 and 68.3% in 1994.Predation was higher in the upper part of the soil andthen progressively decreased with increasing depth.The hoopoe may also prey on cocoons containing pupaeinfected by pathogenic fungi or parasitoids. Anexclosure experiment showed that the bird mayinterfere with these other mortality factors. Threepupal parasitoids, the ichneumonids Erigorgusfemorator Aubert and Coelichneumon rudis (Boyerde Fonscolombe) and the pteromalid Conomoriumpityocampae Graham, were significantly less frequentin the exposed sites. The fungus Beauveriabassiana (Balsamo) Vuillemin and the most abundantpupal parasitoid, Villa brunnea Becker (Diptera:Bombyliidae), caused the highest pupal mortality atboth protected and exposed sites. The bird seems to bean incompletely additive mortality factor acting onthe pest and it can also alter the relativemortalities imposed by the other natural enemies.
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  • 27
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    Entomologia experimentalis et applicata 71 (1994), S. 185-192 
    ISSN: 1570-7458
    Keywords: Lepidoptera ; Pyralidae ; Indianmeal moth ; semiochemicals ; attraction ; oviposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Substrates contaminated by wandering fifth instar larvae ofPlodia interpunctella (Hübner) (Lepidoptera: Pyralidae) elicit oviposition by conspecific female moths, and larval rearing diet enhances oviposition and also induces upwind flight. Two-choice oviposition assays determined that four-day-old gravid femaleP. interpunctella preferred to lay eggs on dishes containing cornmeal-based rearing diet compared to empty dishes. Pieces of cheesecloth contaminated by fifth instar larvae elicited more oviposition than untreated cheesecloth or dishes with food. The combination of larval contamination and food was preferred over food only or larval contamination only in both two- and four-choice experiments. The factor(s) in larval contamination responsible for eliciting oviposition in female moths was extracted in hexane, confirming that organic semiochemicals are responsible for the effect. The oviposition-eliciting activity of larval contamination was retained on cheesecloth for up to 30 days following treatment with larvae, suggesting the active component(s) is stable and of low relative volatility. In two-choice windtunnel bioassays female moths initiated flight only when rearing food was present in one of the treatments, and they displayed the highest landing responses to a combination of larval contamination and food. Earlier work onP. interpunctella and related pyralid species found that larval contamination due to secretions from the mandibular glands acted as both a spacing pheromone for wandering larvae and as a kairomone for host-seeking parasitoid wasps. The present study suggests that the same or a similar secretion acts as an oviposition-eliciting pheromone for conspecific females.
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  • 28
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    Entomologia experimentalis et applicata 72 (1994), S. 173-180 
    ISSN: 1570-7458
    Keywords: feeding performance ; diapausing propensity ; genetic correlation ; heritability ; host-range ; insect-plant interactions ; Lepidoptera ; Tortricidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Evolutionary constraints on the ability of herbivores to efficiently use a set of phytochemically similar hosts, while maintaining a high performance on phytochemically different hosts, are central in explaining the predominance of host specialization in phytophagous insects. Such feeding trade-offs could be manifested within insect populations as negative genetic correlations in fitness on different host species. We tested the hypothesis that feeding trade-offs were present within a population of the obliquebanded leafroller,Choristoneura rosaceana (Harris). Components of fitness were measured in families originating from an apple orchard that were fed on four host-plant species in the laboratory. Under the conditions of this experiment, all across-host genetic correlations were strongly positive, suggesting that this population comprised true generalists. With the exception of diapausing propensity, the heritability of the fitness components tended to be lower in caterpillars fed on apple leaves than in insects fed other hosts. This suggests a constraint on the selective response of the fitness components in the orchard environment.
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  • 29
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    Entomologia experimentalis et applicata 72 (1994), S. 25-31 
    ISSN: 1570-7458
    Keywords: diapause induction ; photoperiod ; temperature ; Phyllonorycter blancardella ; spotted tentiform leafminer ; Lepidoptera ; Gracillariidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The role of photoperiod and temperature in the induction of overwintering diapause inPhyllonorycter blancardella (F.) (Lepidoptera: Gracillariidae) was examined in the laboratory and field using leafminers from commercial apple orchards in Ontario, Canada.P. blancardella exhibited a long-day response to photoperiod: long daylengths resulted in uninterrupted development whereas short daylengths induced diapause. The estimated critical photoperiod for diapause induction was L14.25∶D9.75. The larvae of leafminers destined to enter diapause took ca. 3× longer to complete development than the larvae of non-diapausing leafminers. The development prolonging effect of photoperiod decreased with decreasing daylength. Temperature modified the diapause inducing effect of photoperiod. At L14.25∶D9.75, diapause incidence was similar at 15 and 20°C but was lower at 25°C. Photoperiod also altered the normal relationship between development rate and temperature. At L14.25∶D9.75, the duration of larval development of diapausing leafminers was similar at 15, 20 and 25°C. Temperature alone is unlikely to have a role in the induction of diapause because leafminers exposed to natural late summer and fall temperature regimes and L16∶D8 did not enter diapause.
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  • 30
    ISSN: 1570-7458
    Keywords: Lepidoptera ; Pyralidae ; Ectomyelois ceratoniae ; carob moth ; host-finding ; Phoenix dactylifera ; dates ; gas chromatography-electrophysiology ; mass spectrometry ; wind tunnel ; attraction ; volatiles ; headspace
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four volatile compounds emitted from fungus-infected date fruit,Phoenix dactylifera L., were identified using coupled gas chromatographic-electroantennographic recordings, coupled gas chromatographic-mass spectrometric analysis, electroantennographic assays of synthetic standards, and wind tunnel bioassays. These compounds were ethyl hexanoate, ethanol, acetaldehyde, and 2-phenylethanol. Wind tunnel bioassays showed that ethyl hexanoate was capable of stimulating upwind flight and landing on the source by mated female carob moths,Ectomyelois ceratoniae (Zeller). Addition of both ethanol and acetaldehyde to ethyl hexanoate resulted in an increase in attraction to a level similar to that found for date fruits. No such effect was noted for additions of 2-phenylethanol at the dosages tested. In this study, it appears that ethyl hexanoate is a dominant olfactory stimulant and attractant for mated female carob moths, and represents a novel compound with regard to previously identified lepidopteran host odor attractants.
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  • 31
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    Entomologia experimentalis et applicata 70 (1994), S. 295-298 
    ISSN: 1570-7458
    Keywords: forest tent caterpillar moth ; Malacosoma disstria ; fluctuating asymmetry ; survival ability ; pest management ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fluctuating asymmetry of the first tarsal segment of the proleg of the forest tent caterpiller mothMalacosoma disstria Hbn. (Lepidoptera: Lasiocampidae) was significantly inversely related to survival ability in the lab. The monitoring of population levels of fluctuating asymmetry could have important implications in pest management of this and other species by providing an indication of the health of a population.
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  • 32
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    Entomologia experimentalis et applicata 71 (1994), S. 33-39 
    ISSN: 1570-7458
    Keywords: Helicoverpa zea ; Noctuidae ; Lepidoptera ; Eucelatoria bryani ; Tachinidae ; Diptera ; host-parasitoid relationship ; host regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The parasitoidEucelatoria bryani Sabrosky regulates the larval behavior of its hostHelicoverpa zea (Boddie). Parasitized third, fourth and fifth instars burrow into the soil 0.7–3.4 days earlier than unparasitized larvae that normally enter the soil to pupate at the end of the fifth and final larval instar. Parasitized third instars molt once then burrow as fourth instars, one instar earlier than normal. WhenE. bryani pupariated on the soil surface in the field, none survived to the adult stage. However,E. bryani adults emerged from 49.2% of hosts that had burrowed into the soil. By accelerating the timing ofH. zea burrowing behavior and causing host larvae to enter the soil before death,E. bryani ensures its pupariation in an environment with improved protection against natural enemies and lethal temperatures.
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  • 33
    ISSN: 1570-7458
    Keywords: Lepidoptera ; Papilionidae ; Zerynthia ; Aristolochia ; herbivory ; phenology ; nutritional indices
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The main host plants of the butterflyZerynthia rumina L. (Lepidoptera: Papilionidae) in southern Spain occur in different habitats and in general do not grow sympatrically. Therefore, each single local butterfly population uses the particular host available within its range.Aristolochia longa L. is a tuberous perennial herb available only in the spring, whileA. baetica L. is an evergreen perennial vine with indeterminate growth. However, because of the toughness of older leaves, newly hatched larvae feed only on new leaves ofA. baetica, and most of these leaves are produced well before the larvae hatch. In laboratory experiments, caterpillars feeding on either new or matureA. longa leaves grew faster and converted food into biomass more efficiently than those feeding on newA. baetica leaves. These differences are related to variation in nutritional quality among the host plants. Estimates of butterfly abundance were lower in sites whereZ. rumina usesA. baetica, compared with those where the host isA. longa. The potential differential effect of these two food plants on the densities of local butterfly populations relying on them is discussed here.
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  • 34
    ISSN: 1570-7458
    Keywords: Lepidoptera ; Tortricidae ; Choristoneura fumiferana ; juvenile hormone analog ; fenoxycarb ; postembryonic development
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  • 35
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    Journal of insect behavior 7 (1994), S. 605-632 
    ISSN: 1572-8889
    Keywords: Heliothis ; Lepidoptera ; Noctuidae ; pheromone ; visual feedback
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract MaleHeliothis virescens (F.) (Lepidoptera: Noctuidae) were made to fly into a uniformly white and translucent tube within a large wind tunnel while responding to sex pheromone. Different visual patterns placed within the tube greatly affected the ability of the male moths to maintain upwind progress or remain oriented to the wind while in contact with the plume. Over 89% of males attempting to fly through a blank tube, lacking visual patterns, became disoriented, the males gaining or losing altitude and repeatedly hitting the sides of the tube. Patterns of 20–40 dots placed on the sides of the tube at or slightly above plume level resulted in high levels of sustained upwind flight (47–74%) relative to patterns placed directly below (30–40%), directly above (35%), or slightly below the level of the flight path (26–44%). Optimal upwind progression in pheromone-responding males occurred when image motion could be resolved both transversely (T), orthogonally to the longitudinal axis of the body relative to the horizontal plane of the environment, and longitudinally (L), along the body axis. Even very sparse patterns (single rows of dots) could elicit high levels of sustained upwind flight (53–63%) when positioned within the tube such that the males' movements would create both L and T image motion. However, successful negotiation of the tube was also unexpectedly facilitated by patterns apparently providing no horizontal transverse component for flying males but providing longitudinal flow while centering the moth in the plume through a symmetrical left-right input (4–40%).
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  • 36
    ISSN: 1572-8889
    Keywords: parasitoid ; foraging behavior ; Hymenoptera ; Cotesia ; Lepidoptera ; Pieris ; host location ; solitary ; gregarious ; specialist ; generalist
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the present study we apply a comparative approach, in combination with experimentation, to study behavior of two parasitoid species that attack caterpillar hosts with different feeding strategies (gregarious or solitary). In a semifield setup, consisting of clean cabbage plants and plants infested with one of two host species, the foraging behavior of the specialistCotesia rubecula, on obligate parasitoid of solitarily feedingPieris rapae larvae, was compared to that of the generalistCotesia glomerata, a polyphagous parasitoid of several Pieridae species (mainly the gregariously feedingPieris brassicae).Cotesia glomerata displayed equal propensity to search for and parasitize larvae of both host species. AlthoughC. glomerata exhibited a relatively plastic foraging behavior in that it searched differently under different host distribution conditions, its behavior seems more adapted to search for gregariously feeding hosts. Females exhibited a clear “area-restricted” search pattern and were more successful in finding the gregariously feeding caterpillars.Cotesia rubecula showed a higher propensity to search forP. rapae than forP. brassicae, i.e., females left the foraging setup significantly earlier when their natural hostP. rapae was not present.C. rubecula showed a more fixed foraging behavior, which seems adapted to foraging for solitarily feeding host larvae. In a setup with onlyP. rapae larvae, the foraging strategies of the two parasitoid species were quite similar. In a choice situationC. glomerata did not show a preference for one of the host species, whileCotesia rubecula showed a clear preference for its natural host species. The latter was shown by several behavioral parameters such as the number of first landings, allocation of search time, and percentage parasitization.
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  • 37
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    Journal of insect behavior 7 (1994), S. 885-889 
    ISSN: 1572-8889
    Keywords: Lepidoptera ; Noctuidae ; Mamestra brassicae ; male scents ; hair-pencils ; courtship
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  • 38
    ISSN: 1572-8889
    Keywords: Tanacetum vulgare ; Tansy ; Lobesia botrana ; European grapevine moth ; Asteracea ; Lepidoptera ; Tortricidae ; oviposition ; behavior ; nonhost plant ; semiochemicals ; plant odor ; olfaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Females ofLobesia botrana Den. et Schiff. (Lepidoptera Tortricidae) are attracted in natural conditions by volatiles released by a nonhost plant: tansy (Tanacetum vulgare L.; Asteracea). We have shown that both tansy flowers and their odor inhibit oviposition behavior and mating behavior and reduce adult longevity. The mean number of eggs laid per female isolated with tansy flowers was reduced by up to 50% every 2 days during the 6 days of exposure. This reduction was maintained after the tansy was removed. In the presence of tansy essential oil, the egg-laying reduction ranged from about 30 to 80% according to the odor concentration. The number of spermatophores found in females isolated with tansy flowers was also reduced twofold compared to the control treatment, indicating that the presence of tansy reduced mating activity. This mating activity is strongly reduced, by two-thirds, when adults face the highest dose of essential oil compared to controls. The number of eggs laid by the controls cannot be explained by the number of spermatophores. Therefore, the reduction in oviposition has been attributed to the presence of tansy flowers or to the tansy odor. Tansy flowers and tansy odor increased male mortality during the exposure (10% in the control, 50% in the tansy treatment, and up to 98% in the odor treatment). The highest rates of male mortality occurred during the 4- to 6-day period of exposure to flowers or odor. Repellence resulting in sustained locomotor activity is a possible cause of such a mortality. Female mortality was increased only in response to the highest dose of odor. This increase might be due to egg retention, and not directly to a plant effect. We discuss the effects of tansy flower odor on different patterns relative to the reproductive behavior ofL. botrana and, especially, on oviposition behavior in the ecological context of plant selection and polyphagy.
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  • 39
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    Cellular and molecular life sciences 50 (1994), S. 176-181 
    ISSN: 1420-9071
    Keywords: Cardiac glycoside loss ; Danaus plexippus ; aging ; breakdown of chemical defense ; three trophic level interactions ; automimicry ; Lepidoptera ; Asclepias
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Monarch butterflies (Danaus plexippus) are unpalatable to various vertebrate predators because their larvae sequester bitter and emetic cardiac glycosides (CGs) from milkweed plants (Asclepias spp.). Here we show that the concentration of the defensive CGs decrease as individual butterflies age, regardless of the CGs' initial amounts or specific chemical structures. Consequently, individual monarch butterflies can change from being unpalatable models to palatable mimics during their lifetime. Since monarchs breed continuously over the spring and summer in North America, freshly emerged adult butterflies may serve as noxious models for older individuals which become automimics as they age.
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    Chemoecology 5-6 (1994), S. 75-77 
    ISSN: 1423-0445
    Keywords: larval host plants ; distribution ; Lepidoptera ; Noctuidae ; Othreis fullonia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The adult fruit piercing moth,Othreis fullonia, a native of the indo-Malaysian region, causes severe damage to fruits grown throughout the tropical and subtropical belt from Africa through Asia and Australia to the Pacific Islands. Plants of the family Menispermaceae and the genusErythrina (Fabaceae) serve as larval hosts but the adult moths prefer Menispermaceae plants for oviposition. In Africa, Asia and Australia, the moth does not lay eggs onErythrina since members of the Menispermaceae are abundant. However in the insular Pacific region, where most islands have few or no species of Menispermaceae, the introduced fruit piercing moth utilizesErythrina as an alternate larval host, and either depletes, endangers or causes the possible extinction of Menispermaceae.
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  • 41
    ISSN: 1423-0445
    Keywords: oviposition ; stimulants ; deterrents ; glucosinolates ; Lepidoptera ; Pieridae ; Pieris rapae ; Pieris napi oleracea ; Alliaria petiolata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Differential acceptance of garlic mustard,Alliaria petiolata byPieris rapae L. andP. napi oleracea is explained by their differential sensitivities to oviposition stimulants and deterrents in the plant. Fractions containing the stimulants and deterrents were isolated by solvent partitioning between water and n-butanol and by open-column chromatography followed by HPLC.P. napi oleracea showed no preference when offered a choice ofA. petiolata or cabbage, but was strongly stimulated to oviposit by post-butanol water extracts ofA. petiolata. The most abundant glucosinolate in this extract was identified as sinigrin, which could explain the high degree of stimulatory activity.P. rapae preferred cabbage plants overA. petiolata, and the relatively low stimulatory activity was also associated with the glucosinolate-containing aqueous extract. However, this species was strongly stimulated by a fraction that contained small amounts of glucotropaeolin along with unknown compounds. Deterrents to both species were found in the butanol extract fromA. petiolata, andP. napi oleracea was more sensitive thanP. rapae to these deterrents. Some HPLC fractions from the BuOH extract were strongly deterrent toP. napi oleracea, but were inactive toP. rapae. The ecological significance of these behavioral differences between the twoPieris species is discussed.
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  • 42
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    Chemoecology 5-6 (1994), S. 127-138 
    ISSN: 1423-0445
    Keywords: sequestration ; defence substances ; toxic substances ; pheromones ; host selection ; aristolochic acids ; pyrrolizidine alkaloids ; grayanotoxins ; cyanoglycosides ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A number of aposematic butterfly and moth species sequester toxic substances from their host plants. Some of these insects can detect the toxic compounds during food assessment. Some pipevine swallowtails use aristolochic acids among the host finding cues during oviposition and larval feeding and accumulate the toxins in the body tissues throughout all life stages. Likewise, a danaine butterfly,Idea leuconoe, which sequesters high concentrations of pyrrolizidine alkaloids in the body, lays eggs in response to the specific alkaloid components contained in the apocynad host. Insect species sharing the same poisonous host plants may differ in the degree of sequestration of toxins. Two closely ralated aposematic geometrid moth species,Arichanna gaschkevitchii andA. melanaria, sequester a series of highly toxic diterpenoids (grayanotoxins) in different degrees, while a cryptic geometrid species,Biston robstus, does not sequester the toxins, illustrating the diversity in adaptation mechanisms even within the same subfamily. By contrast, a number of lepidopteran species store the same compounds though feeding upon taxonomically diverse plant species. A bitter cyanoglycoside, sarmentosin, was characterised from several moth species in the Geometridae, Zygaenidae and Yponomeutidae, and from the apollo butterflies,Parnassius spp. (Papilionidae), although each species feeds on different groups of plants. Interspecific similarities and differences in life history and ecology are discussed in relation to variable characteristics of sequestration of plant compounds among these lepidopteran insects.
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  • 43
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    Chemoecology 5-6 (1994), S. 101-117 
    ISSN: 1423-0445
    Keywords: cardenolides ; cardiac glycosides ; chemical defence induction ; latex ; parasitism ; predation ; sequestration ; Insecta ; Diptera ; Tachinidae ; Lepidoptera ; Nymphalidae ; Danainae ; Danaus plexippus ; Asclepiadaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The contribution of Miriam Rothschild to the “monarch cardenolide story” is reviewed in the light of the 1914 challenge by the evolutionary biologist, E.B. Poulton for North American chemists to explain the chemical basis of unpalatability in monarch butterflies and their milkweed host plants. This challenge had lain unaccepted for nearly 50 years until Miriam Rothschild took up the gauntlet and showed with the help of many able colleagues that monarchs are aposematically coloured because they sequester toxic cardenolides from milkweed host plants for use as a defence against predators. By virtue of Dr Rothschild's inspiration and industry, and subsequently that of Lincoln Brower and his colleagues, this tritrophic interaction has become a familiar paradigm for the evolution of chemical defences and warning colouration. We now know that the cardenolide contents of different milkweeds vary quantitatively, qualitatively and spatially, both within and among species and we are starting to appreciate the implications of such variation. However, as Dr Rothschild has pointed out in her publications, cardenolides have sometimes blinded us to reality and it is curious how little evidence there is for a defensive function to cardenolides in plants — especially against adapted specialists such as the monarch. Thus the review will conclude with a discussion of the significance of temporal variation and induction of cardenolide production in plants, the “lethal plant defence paradox” and an emphasis on the dynamics of the cardenolide-mediated interaction between milkweeds and monarch larvae.
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  • 44
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    Chemoecology 5-6 (1994), S. 139-146 
    ISSN: 1423-0445
    Keywords: biochemistry of plants ; sequestration by insects ; transformation by insects ; pyrrolizidine alkaloids ; alkaloidN-oxides ; Asteraceae ; Senecio ; Lepidoptera ; Arctiidae ; Tyria ; Creatonotos
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Among alkaloids the pyrrolizidine alkaloids (PAs) play a unique role in the interactions between plants and adapted insects. InSenecio spp. (Asteraceae) PAs are synthesized in the roots as alkaloidN-oxides which are specifically translocated into shootsvia the phloem-path and channeled to the preferred sites of storage (e.g. inflorescences) where they are stored in the cell vacuoles. In differentSenecio spp. senecionineN-oxide is produced as the common product of biosynthesis, which subsequentlyvia a number of simple but specific reactions is transformed into typical speciesspecific PA-patterns. Insects from diverse taxa sequester PAs for their own defense. Lepidopterans (e.g. arctiids such asTyria jacobaeae andCreatonotos transiens) may hydrolyze plant acquired ester-PAs and convert the resulting necine base into insect-specific PAs by esterification with an acid of their own metabolism. Adapted arctiids and the grasshopperZonocerus take up PAs in the state of the tertiary amine.N-Oxides are reduced in the guts prior to uptake. In the bodies the tertiary PAs are rapidlyN-oxidized by a specific mixed-function oxigenase and are maintained in theN-oxide state. The importance of the reversible interconversion of the nontoxicN-oxide (pro-toxine) into the toxic tertiary alkaloid is discussed as the specific feature of PAs in plant-insect interactions.
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  • 45
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    Chemoecology 5-6 (1994), S. 167-171 
    ISSN: 1423-0445
    Keywords: inhibition ; indigestibility ; defence ; alkaloid ; glycosidases ; Lepidoptera
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    Topics: Biology
    Notes: Summary Glycosidase inhibitors are widespread in plants and can be sequestered by Lepidoptera, for which they can presumably serve as defences by making the insects indigestible to a range of potential predators. As a result of this study of eight British species of moth and butterfly it was found that glycosidase inhibitors in the insects could then be detected in the larval food plants which were not previously known to contain them; however, some were only detectable in the plants after concentration. In some cases the inhibition of specific glycosidases by Lepidoptera was detected even though the insects had not apparently acquired them from their food plants. Inhibition ofβ-N-acetylglucosaminidase was observed in most of the adult Lepidoptera analysed but further work is required to identify the inhibitors, though they are likely to be nitrogen-containing compounds. Weak anti-HIV activity was also observed in the glycosidase-inhibiting fractions ofAcherontia atropos and the plantUrtica dioica.
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  • 46
    ISSN: 1423-0445
    Keywords: predation ; plant-insect interactions ; tritrophic level interactions ; iridoid glycosides ; catalpol ; Lepidoptera ; Nymphalidae ; Junonia coenia ; Hymenoptera ; Formicidae ; Camponotus floridanus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We investigated the role of the iridoid glycoside, catalpol, as a deterrent to the predator,Camponotus floridanus. Four laboratory colonies of this ant were offered buckeye caterpillars (Junonia coenia: Nymphalidae) raised on diets with and without catalpol. The same colonies were offered sugar-water solutions containing varying concentrations of catalpol, in both no-choice and choice tests. Regardless of diet, buckeye caterpillars appeared to be morphologically protected from predation by the ants, possibly because of their large spines or tough cuticle. However, buckeyes raised on diets with catalpol had high concentrations of catalpol in their hemolymph; extracts of this high-catalpol hemolymph proved to be an effective deterrent to the ants. When starved ants were not given the choice of food items, they were more likely to consume sucrose solutions that contained 5 mg catalpol/ml or 10 mg catalpol/ml than they were to consume solutions with 20 mg catalpol/ml. When they were given a choice of sugar solution or a sugar solution containing catalpol, the ants avoided solutions with catalpol at any of these concentrations. Ant colony responses to catalpol in sucrose solutions varied considerably over time and among colonies.
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  • 47
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    Chemoecology 5-6 (1994), S. 26-36 
    ISSN: 1423-0445
    Keywords: chemoreception ; olfaction ; plant volatiles ; electroantennogram ; combined GC-EAG ; evolutionary adaptation ; Lepidoptera ; Papilionidae ; Papilio polyxenes ; Papilio machaon hippocrates ; Papilio troilus ; Apiaceae ; Daucus carota ; Pastinaca sativa ; Asteraceae ; Artemisia dracunculus
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    Notes: Summary Antennae of femalePapilio butterflies perceive many volatile plant constituents with widely differing, constituent-specific sensitivities. We compared the responses of threePapilio species to volatiles from host and non-host plants to assess species-specificity and the degree of evolutionary conservatism in olfactory responses. Since previous studies had demonstrated that the polar constituents in odor fromDaucus carota stimulate oviposition behavior inPapilio polyxenes, we collected headspace volatiles fromD. carota, Pastinaca sativa (both Apiaceae) andArtemisia dracunculus (Asteraceae) and separated the polar fraction of these volatiles by gas chromatography. GC-coupled electroantennograms (GC-EAG) were recorded from the speciesPapilio polyxenes, P. machaon hippocrates andP. troilus. In addition, the responses of the three species to five compounds known as generally occurring constituents of plant odor were recorded. The relative sensitivities for these compounds were nearly identical in all threePapilio species. The response spectra to the separated plant volatiles also showed considerable similarities among the species. From the limited set of GC peaks evoking a response in one of the species, 64% (D. carota), 44% (P. sativa) and 29% (A. dracunculus) also evoked a response in both of the other species. The responses of the two closely related Apiaceae feeders (P. polyxenes, P. m. hippocrates) to volatiles fromD. carota were more similar to each other than was either to the response ofP. troilus, which feeds on Lauraceae. However, this was not true for the responses to volatiles fromP. sativa. The least congruence among the three species was found in the responses to volatiles fromA. dracunculus, a non-host for all of them. The differences and similarities found in the response profiles of the threePapilio species are discussed with respect to evolutionary adaptation to host odor versus evolutionary conservatism in adaptation of olfactory receptors.
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  • 48
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    Journal of chemical ecology 20 (1994), S. 231-238 
    ISSN: 1573-1561
    Keywords: Trichoplusia ni ; Lepidoptera ; Noctuidae ; sex pheromone ; behavior ; evolution ; sexual selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Male cabbage looper moths,Trichoplusia ni, from two colonies in which all females express an abnormal sex pheromone production phenotype were evaluated in a laboratory wind tunnel for upwind flight responses to the normal and abnormal sex pheromones. The abnormal sex pheromone blend consisted of 20 times as much (Z)-9-tetradecenyl acetate and 30-fold less (Z)-5-dodecenyl acetate compared to the normal pheromone blend. Initially, these males exhibited poor behavioral responses to the abnormal sex pheromone and maximum responses to the normal pheromone blend, indicating that there was no linkage between signal production and response. After 49 generations of laboratory rearing, males from the mutant colonies maintained good responses to the normal pheromone and increased their behavioral response to the abnormal sex pheromone to the same levels as for the normal pheromone. Over the same period, normal males maintained their preference for the normal pheromone. These results indicated that evolution had occurred in mutant colonies in favor of greater male responsiveness to the abnormal sex pheromone, resulting in the broadening of the response spectrum to pheromone blend ratios. This evolution presumably resulted from a mating advantage to those males that did not discriminate against mutant-type females in the mutant colonies.
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  • 49
    ISSN: 1573-1561
    Keywords: Pieris rapae ; Pieris napi oleracea ; Lepidoptera ; Pieridae ; Barbarea vulgaris ; oviposition ; stimulants ; glucosinolates ; glucobarbarin ; glucobrassicin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The closely related butterflies,Pieris rapae andP. napi oleracea, readily laid eggs onBarbarea vulgaris in greenhouse cages. When offered a choice between cabbage andB. vulgaris, P. rapae showed no preference, butP. napi oleracea preferredB. vulgaris. Bioassays of extracts ofB. vulgaris foliage revealed the presence of oviposition deterrent(s) in l-butanol extracts as well as stimulants in the postbutanol water extracts. However, the deterrent effect was apparently outweighed by the strong stimulatory effect in the whole plants. The postbutanol water extract was preferred over an equivalent cabbage extract by both species, but more significantly in the case ofP. napi oleracea. The stimulants were isolated by open column chromatography and HPLC, and the activity was associated with three glucosinolates.P. napi oleracea was more sensitive thanP. rapae to the natural concentration of compounds1 and3, whereas both species were strongly stimulated to oviposit by natural concentrations of compound2. Compounds1 and2 were identified as (2R)-glucobarbarin and (2S)-glucobarbarin, respectively, and3 was identified as glucobrassicin, on the basis of their UV, mass, and NMR spectra. When the pure compounds were tested at the same concentrations applied to bean plants, the (2R)-glucobarbarin at 0.2 mg/plant was preferred over a standard cabbage extract by both butterfly species. However, at a dose of 0.02 mg/plant,P. rapae preferred the cabbage extract whereasP. napi oleracea still preferred the (2R)-glucobarbarin. No such difference in response of the two species to the same two concentrations of (2S)-glucobarbarin was obtained. The results indicate a distinct difference in sensitivity of these butterflies to the epimers of glucobarbarin, and the differences in behavioral responses of the two butterfly species depend to a large extent on the concentration of stimulant present.
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  • 50
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    Journal of chemical ecology 20 (1994), S. 1039-1051 
    ISSN: 1573-1561
    Keywords: Pieris rapae ; Pieris napi oleracea ; Lepidoptera ; Pieridae ; oviposition ; deterrents ; cardenolides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Oviposition responses ofPieris rapae andP. napi oleracea to 18 cardenolides were compared under the same conditions. Effects of different concentrations of selected cardenolides were also tested. Most of the compounds were deterrent to oviposition by both insects, but to significantly different degrees.P. rapae were strongly deterred by K-strophanthoside, K-strophanthin-β, cymarin, convallatoxin, oleandrin, erysimoside, erychroside, and gitoxigenin. The most deterrent compounds forP. napi oleracea were erychroside, cymarin, erysimoside, convallatoxin, and K-strophanthoside. Strophanthidin-based glycosides were more deterrent than digitoxigenin-based ones, and the number and type of sugar substitutions can have profound effects on activity. Both similarities and contrasts were found in responses ofP. rapae andP. napi oleracea to these cardenolides. Cymarin was equally deterrent to bothPieris species at all concentrations tested. However, when compared withP. rapae, P. napi oleracea was less sensitive to most of the cardenolides.P. napi oleracea was insensitive to K-strophanthin-β and oleandrin at 0.5 × 10−4 M, which were highly deterrent toP. rapae.
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  • 51
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    Journal of chemical ecology 20 (1994), S. 1063-1073 
    ISSN: 1573-1561
    Keywords: Oxime ether ; NMR data ; pheromone mimics ; ESG studies ; structure-response relationships ; turnip moth ; Agrotis segetum Schiff. ; Lepidoptera ; Noctuidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Oxime ether analogs of sex pheromone components of the turnip moth (Agrotis segetum Schiff.) were synthesized by the acidolytic opening of cyclic enol ethers withO-alkyl hydroxylamine hydrochlorides. The compounds varying in chain lengths and in the position of the C=N double bond were studied by electrophysiological single sensillum recordings (electrosen-sillography: ESG). The ESG data indicate in general reduced receptor interaction of all analogs investigated in comparison with natural pheromone components of the turnip moth. The data also show that the grade of decrease of receptor interaction depends on specific structural changes within the molecule. The results demonstrate high complementary pheromone-receptor relationships, predominantly depending on the position of the unsaturated group in the chain, whereas analogs with other structural changes are still recognized as a pheromone-like compound by the receptor.
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  • 52
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    Journal of chemical ecology 20 (1994), S. 1825-1841 
    ISSN: 1573-1561
    Keywords: Epiphyas postvittana ; Lepidoptera ; Tortricidae ; electroantennogram ; pheromone ; dispenser ; apple ; mating ; disruption ; atmospheric concentration
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The absorption and release of the pheromone ofEpiphyas postvititana (Lepidoptera: Tortricidae),E 11–14: OAc andE,E 9, 11–14: OAc (95:5) by apple leaves was studied using electroantennograms (EAG) and sticky traps baited with pheromone-treated leaves. Leaves exposed to an airstream containing pheromone reached a constant level of pheromone release within 3 min. Release occurred over a period greater than 24 hr, following removal of leaves from the pheromone-saturated environment. Pheromone-treated leaves were effective as lures in sticky traps for at least three nights, although the average catch per night decrease logarithmically with time. In the field, pheromone was detected by EAG on leaves harvested from up to 25 cm away from a central point source of pheromone. The shape of a surface representing equal pheromone re-release from leaves around a central point source was defined by interpolation from a three-dimensional transect. Leaves harvested from 5 cm under the dispensers showed the highest pheromone release rate. Leaves downwind of the dispensers also had higher release of pheromone. In a treated orchard, significantly higher EAG measurements were recorded in the rows of trees that contained dispensers, compared to grass interrows or untreated trees. The implications of foliar pheromone adsorption and release on atmospheric concentrations and insect behavior require further investigation.
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  • 53
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Tortricidae ; Acleris variana ; sex pheromone ; (E)-11,13-tetradecadienal
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract (E)-11,13-Tetradecadienal (E11,13–14:Ald) is the major sex pheromone component of the eastern blackheaded budworm (EBB),Acleris variana (Fern.). The compound was identified in female pheromone gland extracts by coupled gas chromatographic-electroantennographic detection (GC-EAD), coupled GC-mass spectrometry in selected ion monitoring mode, and retention index calculations of candidate pheromone components.E11,13–14:Ald alone as trap bait was very attractive to male EBB. Addition of the corresponding diene alcohol or acetate or both did not enhance attraction. (Z)-11,13-Tetradecadienal in binary combination with (E)-11,13–14:Ald neither enhanced nor reduced trap catches. Increasing the amounts of pheromone from 0.01 to 10 µg increased trap catches, but increase of pheromone quantity above 100 µg proportionately reduced attraction. Stabilization of slowly polymerizingE11,13–14:Ald and development of a sustained, adequate release rate is required for pheromone-based monitoring of EBB populations.
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  • 54
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Noctuidae ; Diachrysia chrysitis ; Diachrysia tutti ; pheromones ; sibling taxa ; electroantennographic responses ; biosynthesis ; cross-attraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The noctuid sibling taxaDiachrysia chrysitis s. str. andD. tutti, of yet uncertain taxonomic status, have previously been shown to possess differences in morphology and to be attracted to different mixtures of the two presumed pheromone components (Z)-5-decenyl acetate and (Z)-7-decenyl acetate. TypicalD. tutti males (clearly broken forewing marking) are known to respond to a 2: 100 mixture of the two isomers, whereasD. chrysitis males (unbroken marking) are attracted to a 100: 10 mixture. We investigated female pheromone production and male electroantennographic (EAG) response inDiachrysia families raised in the laboratory from field-collected gravid females. Extracts of individual females from typicalD. tutti andD. chrysitis families were subjected to gas chromatography with simultaneous flame ionization and electroantennographic detection. All females produced mixtures of Z5- and Z7-10:OAc, but femaleD. chrysitis produced predominantly Z5-10:OAc and the antennae of their brothers responded more strongly to the Z5 peak than to the Z7-10:OAc peak, whereas the opposite was true forD. tutti families. The pheromone components were shown to be biosynthesized from hexadecanoic and tetradecanoic acid, respectively by Z11-desaturation followed by chain shortening, reduction, and acetylation. The EAG responses of males trapped with the typicalD. tutti andD. chrysitis blends, as well as with an intermediate blend, were investigated. Males trapped with theD. tutti mixture almost exclusively had a clearly broken wing marking and showed strongest EAG response to Z7-10:OAc. The intermediate blend and theD. chrysitis mixture gave more mixed catches, but with a prevalence of males with an unbroken (or almost unbroken) wing marking and with a higher mean response to Z5-10:OAc. Some males with typicalD. tutti EAG responses were attracted in the field to theD. chrysitis pheromone. In the flight tunnel someD. chrysitis males were attracted also to theD. tutti mixture. This indicates that cross attraction may take place between the two taxa under natural conditions.
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  • 55
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    Journal of chemical ecology 20 (1994), S. 171-181 
    ISSN: 1573-1561
    Keywords: Codling moth ; Cydia pomonella ; Lepidoptera ; Tortricidae ; communication disruption ; mating disruption ; sex pheromone ; (E,E)-8,10-dodecadien-1-ol ; (E,Z)-8,10-dodecadien-1-ol ; (Z,E)-8,10-dodecadien-1-ol ; dodecan-1-ol ; tetradecan-1-ol
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In a small section of an apple orchard, six traps were placed each in control and test areas and baited with live virgin female codling moths. Gray elastomer septa were used to dispense communication disruptants around the traps. Dyed male codling moths were released in control and test areas, and the numbers of males captured in control and test traps were compared. In 1991, linear regression curves of percent communication disruption versus logarithm of dose were obtained for three compositions: (E,E)-8,10-dodecadien-1-ol, codlemone (1); codlemone + dodecan-1-ol + tetradecan-1-ol (2); and an equilibrium mixture of the four isomers of 8,10-dodecadien-1-ol (30, (61%EE, 14%ZE, 20%EZ, and 5%ZZ). All three regressions gaver 2 values greater than 0.90. At the 95% confidence limits, slopes and intercepts of compositions 1 and 2 were equivalent, and different from that of composition 3, which produced the greatest percentages of disruption at all doses. In 1992, five treatments were compared at a single dose: 1, 3, none (4), (Z,E)-8,10-dodecadien-1-ol (5), (E,Z)-8,10-dodecadien-1-ol (6). Compositions 5 and 6 gave the greatest and similar percentages of disruption and were different from codlemone (1) and 4 (95% confidence), but not from composition 3. Communication disruption produced by composition 3 was greater than (codlemone), which was greater than 4.
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  • 56
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Tortricidae ; Cydia pomonella ; codling moth ; sex pheromone ; halogenated analogs ; isosteric replacements ; EAG ; single sensillum recording ; field trapping ; structure-activity relationship ; codlemone
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Pest monitoring and control of the codling moth,Cydia pomonella L., have been developed using the main pheromone component of this species, (E,E)-8,10-dodecadienol (codlemone). However, the activity of codlemone is not satisfactory for pest control by mating disruption. Thus, we have synthesized halogenated analogs of codlemone to see if they could be used as new agents for pest control of the codling moth. Their biological activity was measured by electrophysiological techniques. In EAG screening, codlemone was the most active compound. F(10,11)-codlemone [(E,E)-10,11-difluoro-8,10-dodecadienol] and Cl-codlemone [(E,E)-11-chloro-8,10-undecadienol] elicited significant EAG responses, F(10,11)-codlemone triggering responses not significantly different from responses to codlemone. EAG cross-adaptation experiments and single sensillum recordings revealed that these compounds were detected by the same receptor neuron type as codlemone. No competitive inhibition with codlemone was observed from nonactive compounds. In field trapping, F(10,11)-codlemone and Cl-codlemone were more attractive to male codling moths than codlemone itself. Possible explanations of this activity are discussed.
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  • 57
    ISSN: 1573-1561
    Keywords: Citrus jambhiri ; Trichoplusia ni ; Lepidoptera ; Noctuidae ; bergapten ; furanocoumarins ; phototoxins ; plant-herbivore interactions ; psoralen ; ultraviolet-B radiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Projected decreases in stratospheric ozone may result in increases in shortwave ultraviolet (UVB) irradiation at the earth's surface. Furanocoumarins, phototoxic compounds found inCitrus jambhiri foliage, increase in concentration when these plants are grown under enhanced UVB. Survivorship schedules ofTrichoplusia ni (Lepidoptera: Noctuidae) caterpillars reared on plants in the presence and absence of enhanced UVB regimes differ significantly; larvae develop more slowly in early life when reared on plants exposed to increased UVB. This same developmental pattern is observed whenT. ni larvae are reared on artificial diets amended with ecologically appropriate amounts of furanocoumarins. Thus, anthropogenically derived changes in stratospheric ozone and concomitant changes in UV light quality at the earth's surface may influence ecological interactions between insects and their host plants by altering secondary metabolism and hence foliage quality for herbivores.
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  • 58
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    Journal of chemical ecology 20 (1994), S. 639-650 
    ISSN: 1573-1561
    Keywords: Soybean ; lipoxygenase ; peroxidase ; polyphenol oxidase ; trypsin inhibitor ; ascorbate oxidase ; oxidative stress ; Lepidoptera ; Noctuidae ; Helicoverpa zea ; corn earworm ; Cerotoma trifurcata ; bean leaf beetle ; Coleoptera ; Chrysomelidae ; Spissistilus festinus ; three-cornered alfalfa hopper ; Homoptera ; Membracidae ; induced resistance ; interspecific competition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Variation in induced responses in soybean is shown to be dependent, in part, upon herbivore species. Herbivory by the phloem-feeding three-cornered alfalfa hopper caused increases in the activities of several oxidative enzymes including lipoxygenases, peroxidases, ascorbate oxidase, and polyphenol oxidase. Bean leaf beetle defoliation caused increased lipoxygenase activity, but had little effect upon peroxidase, polyphenol oxidase, ascorbate oxidase, or trypsin inhibitor levels in either field or greenhouse studies. In one field experiment, prior herbivory by the bean leaf beetle subsequently reduced the suitability of foliage to the corn earwormHelicoverpa zea. The contribution of these findings to emerging theories of insect-plant interactions is discussed.
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  • 59
    ISSN: 1573-1561
    Keywords: Sex pheromone ; (E)-11-hexadecenal ; (E)-11-hexadecen-1-yl acetate ; Palpita unionalis ; Lepidoptera ; Pyralidae ; Pyrustinae ; Anisodes sp. ; Geometridae ; jasmine moth
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract (E)-11-Hexadecenal and (E)-11-hexadecen-1-yl acetate were found in abdomen tip extracts from females of the jasmine mothPalpita unionalis (Hübn). The identification was based on capillary GC analyses, mass spectrometry, and laboratory and field tests. In laboratory bioassays, both components elicited a low level of upwind flight by males. The two components when tested separately in the field were inactive, but the blend of the two components at a ratio of (3:7) was highly attractive to males. Traps baited with 1 mg of the two-component blend were competitive to traps baited with five virgin females. The addition of Z isomers components reduced male capture.
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  • 60
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Tortricidae ; Spilonota ocellana ; eye-spotted bud moth ; sex pheromone ; (Z)-8-tetradecenyl acetate and (Z)-8-tetradecenyl alcohol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Response of male eye-spotted bud moth,Spilonota ocellana (Denis and Schiffermüller), to different ratios of synthetic sex pheromone components, (Z)-8-tetradecenyl acetate (Z8-14:OAc) and (Z)-8-tetradecenyl alcohol (Z8-14:OH), were compared in four North American locations and in one location in The Netherlands. In British Columbia, Nova Scotia, Michigan, and The Netherlands, a 99:1 blend ofZ8-14:OAc andZ8-14:OH captured significantly more maleS. ocellana thanZ8-14:OAc alone or binary blends containing 10–50%Z8-14:OH. In Ontario, where population sizes were low compared to the other four locations, trends in trap catches were similar, and there was no indication that maleS. ocellana responded differently to the tested pheromone blends. A 99:1 blend ofZ8-14:OAc andZ8-14:OH should be most effective in pheromone-based control programs ofS. ocellana in North America and in The Netherlands. Our results confirm earlier studies that a 99:1 blend ofZ8-14:OAc andZ8-14:OH captures significantly more maleS. ocellana thanZ8-14:OAc alone. However, our finding that a 99:1 blend ofZ8-14:OAc andZ8-14:OH is significantly more attractive than binary blends containing 10–50%Z8-14:OH differs from previous findings in Germany and Switzerland.
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  • 61
    ISSN: 1573-1561
    Keywords: Plutella xylostella ; Lepidoptera ; Plutellidae ; Brassica ; host plant attraction ; EAG ; bioassay ; host plant location ; plant volatiles ; mustard oils ; isothiocyanates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Olfactory attraction of female diamondback moths (Plutella xylostella) to odors of intact and homogenized host plants, as well as individual compounds characteristic of host plants, were investigated by behavioral and electrophysiological methods. Moths were attracted to odors ofBrassica juncea andB. napus seedlings in a Y-tube bioassay. Solvent fractions of homogenizedB. juncea leaves were attractive to moths whether or not isothiocyanates (IC) were present. Moths were attracted in Y-tube bioassays and to field traps baited with individual ICs. Volatiles fromB. juncea andB. napus elicited an electroantennogram (EAG) response and were attractive in the Y-tube bioassay. Allyl IC was shown to be the attractive component in homogenized plant volatiles but was found to be virtually absent from intact plant volatiles. Gas chromatographic fractionation of intact plant volatiles revealed a terpene-containing fraction to be most attractive to the moths. We were unable to isolate individual attractive compounds from this fraction. Our results suggest that certain elements of this fraction, possibly in combination, are important olfactory cues for host-plant finding by the diamondback moth with mustard oils playing an important and possibly synergistic role, particularly when plants are damaged.
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  • 62
    ISSN: 1573-1561
    Keywords: Cucurbitaceae ; Cucurbita pepo ; Insecta ; Lepidoptera ; Pyralidae ; Diaphania ; oviposition ; nitidalis ; pickleworm moth ; egg laying ; visitations ; stimulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Fourteen volatile compounds occurring in leaf trichomes of yellow squash (Cucurbita pepo L. cv. Early Prolific Straightneck) were identified. These compounds accounted for 83.5% of the volatile matrix. Ubiquitous constituents of the epidermis (myristic, palmitic, and stearic acids,n-tricosane, andn-pentacosane) accounted for 73.7%; these compounds were not bioassayed. The volatileso-,m-, andp-xylene, toluene, 2-heptanone, (R)-(+)- and (S)-(−)-limonene, and germacrene D were tested for their influence on attraction and oviposition by the pickleworm moth (Diaphania nitidalis Stoll.). No single compound, except germacrene D, was attractive. (R)-(+)-Limonene and 2-heptanone were weakly repellent. Mixtures of the highly volatile fractions were as attractive as volatiles emanating from whole, intact leaves. Oviposition levels on treated artificial sites corresponded with levels of visitation. Oviposition was significantly stimulated by “whole-leaf” volatiles, and (S)-(−)-limonene caused a slight but significant reduction.
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  • 63
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    Journal of chemical ecology 20 (1994), S. 2127-2138 
    ISSN: 1573-1561
    Keywords: Attacus atlas ; biogenic amines ; chemical defense ; defensive glands of caterpillars ; histamine ; Lepidoptera ; Saturniidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The ability ofAttacus atlas caterpillars to spray a defensive secretion seems to be due to the fine structure of the integumental glands that produce it. The giant gland cells are fixed to stable cuticular rings surrounding the gland openings and tightly closed by cuticular lids. Probably by increasing hemolymph pressure, the lids are blasted off and the secretion spouts out. The fluid contains several aromatics, biogenic amines (e.g., acetylcholine, histamine), glycerol, and trehalose and exhibits tyrosinase activity. Deterrent effects of caterpillar secretion and hemolymph on predatory ants could be shown. Presumably the spraying process serves to apply the secretion to sensitive sites of vertebrate target organisms.
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  • 64
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Pieridae ; Hymenoptera ; Braconidae ; cabbage ; Brussels sprouts ; behavior ; tritrophic interactions ; green-leaf volatiles ; herbivore-induced synomones ; elicitor ; caterpillar regurgitant ; Brassica oleracea ; Pieris brassicae ; Cotesia glomerata ; parasitoid ; wasp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Induction of plant defense in response to herbivory includes the emission of synomones that attract the natural enemies of herbivores. We investigated whether mechanical damage to Brussels sprouts leaves (Brassica oleracea var.gemmifera) is sufficient to obtain attraction of the parasitoidCotesia glomerata or whether feeding byPieris brassicae caterpillars elicits the release of synomones not produced by mechanically damaged leaves. The response of the parasitoidCotesia glomerata to different types of simulated herbivory was observed. Flight-chamber dual-choice tests showed that mechanically damaged cabbage leaves were less attractive than herbivore-damaged leaves and mechanically damaged leaves treated with larval regurgitant. Chemical analysis of the headspace of undamaged, artificially damaged, caterpillar-infested, and caterpillar regurgitant-treated leaves showed that the plant responds to damage with an increased release of volatiles. Greenleaf volatiles and several terpenoids are the major components of cabbage leaf headspace. Terpenoids are emitted in analogous amounts in all treatments, including undamaged leaves. On the other hand, if the plant is infested by caterpillars or if caterpillar regurgitant is applied to damaged leaves, the emission of green-leaf volatiles is highly enhanced. Our data are in contrast with the induction of more specific synomones in other plant species, such as Lima bean and corn.
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  • 65
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    World journal of microbiology and biotechnology 10 (1994), S. 406-409 
    ISSN: 1573-0972
    Keywords: Bacillus cereus-diarrhoeal-type enterotoxin ; Bacillus thuringiensis ; Coleoptera ; Diptera ; Lepidoptera ; thuringiensin
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract At moderate concentration, 23 of 40 strains of Bacillus thuringiensis isolated from Sweden were toxic to Trichoplusia ni and five were toxic to Aedes aegypti. Five of the strains were toxic to Diabrotica undecimpunctata at high concentration, two were toxic to Heliothis virescens at low concentration and five produced thuringiensin (formerly called β-exotoxin). No strain was toxic towards the beet armyworm Spodoptera exigua at low concentration. Twenty-three of the strains produced a B. cereus-diarrhoeal-type enterotoxin.
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  • 66
    ISSN: 1573-1561
    Keywords: Bombyx mori ; Lepidoptera ; Bombiridae ; silkworm ; larvae ; ecdysone ; 20-hydroxyecdysone ; feeding behavior ; electrophysiology ; sensilla response ; chemoreceptors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The feeding and gustatory responses to ecdysone and 20-hydroxyecdysone were investigated in the silkworm,Bombyx mori. 20-Hydroxyecdysone reduced feeding response strongly in fourth- and fifth-instar larvae, whereas ecdysone had no effect on feeding response. 20-Hydroxyecdysone stimulated the R receptor, the receptor to feeding deterrents, to a great degree. By contrast, ecdysone was much less effective for stimulating the R receptor. These results indicate that ecdysone and 20-hydroxyecdysone have different effects on feeding response due to different interactions with mouthpart chemoreceptors.
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  • 67
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    Journal of chemical ecology 20 (1994), S. 183-198 
    ISSN: 1573-1561
    Keywords: Induced resistance ; protein quality ; Helicoverpa zea ; soybean ; Glycine max ; protease inhibitor ; lipoxygenase ; Lepidoptera ; noctuidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Resistance in soybean toHelicoverpa zea is comprised of both constitutive and inducible factors. In this study, we investigated the induction of resistance byH. zea in both greenhouse and field studies. In a greenhouse experiment, fourth-instarH. zea growth rates were reduced by 39% after 24 hr feeding and by 27% after 48 hr when larvae fed on previously wounded V3 foliage (cv. Forrest) compared with undamaged foliage. In a field study, the weight gain by larvae was more than 52% greater when larvae fed for 72 hr on undamaged R2/R3 soybean plants (cv. Braxton) compared to those that fed on previously wounded plants. A significant component of the induced resistance is due to a decline in the nutritional quality of foliar protein following foliar damage byH. zea. Foliar protein was extracted from damaged and undamaged foliage and incorporated into artificial diets. Larval growth was reduced 26% after four days and 49% after seven days on diets containing protein from damaged plants compared to larvae feeding on foliar protein from undamaged plants. Chemical analyses of protein quality also indicated a decline in quality in damaged plants compared to unwounded plants. Increases in lipoxygenase activity (53%), lipid peroxidation products (20%), and trypsin inhibitor content (34%) were observed in protein from wounded plants. Moreover, a 5.9% loss in free amines and 19% loss in total thiols occurred in protein from wounded plants. Larval feeding causes a significant increase in foliar lipoxygenase activity that varied among genotypes. Lipoxygenase isozymes were measured at pH 5.5, pH 7.0, and pH 8.5 in V3 stage plants of Forrest, Hark, D75-1069, and PI 417061 genotypes. Lipoxygenase activity in each genotype was significantly increased after 72 hr of larval feeding at each pH level tested, with the exception of lipoxygenase isozymes at pH 5.5 in genotype PI 417061. Larval feeding on R2/R3 stage plants (field-grown cv. Braxton) for six days also increased foliar lipoxygenase activity.
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  • 68
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Geometridae ; Lambdina athasaria ; Lambdina fiscellaria fiscellaria ; Lambdina fiscellaria lugubrosa ; sex pheromone ; synergism ; 7,11-dimethylheptadecane ; 7-methylheptadecane ; 5,11-dimethylheptadecane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Two methylated hydrocarbons, 7-methylheptadecane (7) and 7,11-dimethylheptadecane (7,11), are female sex pheromone components of the spring hemlock looper (SHL),Lambdina athasaria (Walker). Compounds extracted from female pheromone glands were identified by coupled gas chromatographic-electroantennographic detection (GC-EAD) and coupled GC-mass spectrometry (GC-MS) in selected ion monitoring mode. In field trapping experiments, (7) and (7,11) by themselves were behaviorally inactive, but in combination attracted numerous male moths. (5,11)-Dimethylheptadecane (5,11) was detected in female SHL pheromone gland extracts, but did not enhance attraction to the binary blend of (7) and (7,11). The sex pheromone of SHL is related to that of congeneric eastern hemlock looper (EHL),Lambdina fiscellaria fiscellaria (Guen.) [(5,11) and 2,5-dimethylheptadecane (2,5)] and western hemlock looper (WHL),L.f. lugubrosa (Hulst) [(5,11), (2,5) and (7)]. Specificity of the pheromonal blend, spatial separation of coseasonal EHL and WHL, and temporal separation of sympatric EHL and SHL contribute to reproductive isolation.
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  • 69
    ISSN: 1573-1561
    Keywords: Cranberry fruitworm ; Lepidoptera ; Pyralidae ; Acrobasis vaccinii ; sex pheromone ; (E,Z)-8,10-pentadecadien-l-ol acetate ; (E)-9-pentadecen-l-ol acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The following compounds and (approximate ratios) were identified in sex pheromone gland extracts of femaleAcrobasis vaccinii Riley by comparison of gas chromatography-mass spectrometric traces with those of synthetic standards: (E,Z)-, (Z,E)-, (Z,Z), and (E,E)-8, 10-pentadecadien-l-ol acetates (100:1:2:12), a dodecen-l-ol acetate (8), (Z)-8-, (Z)-9-, and (E)-9-pentadecen-l-ol acetates (3:23:4), two heptadecen-l-ol acetates (4:4), tetradecyl, pentadecyl, hexadecyl, and heptadecyl acetates (3:15:10:8), dodecan-l-ol (6), tetradecan-l-ol (5), and hexadecan-l-ol (23). The amount of (E,Z)-8, 10-pentadecadien-l-ol acetate (E8,Z10–15:Ac) in the extract was about 0.5 ng/female. Electroantennographic analysis of gas chromatographic fractions of female sex pheromone gland extract showed that the fraction containingE8,Z10–15:Ac elicited the greatest response. Alone,E8,Z10–15:Ac failed to elicit upwind flight of males in flight-tunnel tests, and traps baited with it did not catch males in field experiments. WhenE8,Z10–15:Ac was combined with (E)-9-pentadecen-l-ol acetate (100:4), male upwind flight response in flight-tunnel tests was equivalent to those obtained with extract of female sex pheromone glands (synthetic, 62%; natural, 51%), but the percent of males flying upwind that contacted the source was lower (synthetic, 47%; natural, 88%). The lower percent of source contact elicited by the synthetic pheromone could be a result of the difference in isomer ratios of 8,10–15:Ac in the natural and synthetic pheromone or could indicate that the synthetic pheromone is incomplete. Traps baited with the 100:4 combination caught large numbers of males in field experiments.
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  • 70
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    Journal of chemical ecology 20 (1994), S. 395-405 
    ISSN: 1573-1561
    Keywords: Douglas fir ; Pseudotsuga menziesii ; galactose ; terpenes ; sugars ; carbohydrates ; resistance ; western spruce budworm ; mortality ; Choristoneura occidentalis ; Lepidoptera ; Tortricidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The current year's growth of Douglas fir contains galactose, unusual in that this carbohydrate makes up 78.7% of the total carbohydrate fraction. An agar diet study was undertaken to determine the effects of galactose, other carbohydrates, and terpenes on western spruce budworm larval mortality, growth rate, and adult biomas production. All concentrations of the carbohydrates and terpenes tested, as well as other mineral elements not tested, were typical of the current year's foliage of Douglas fir. In experiment I, the diet containing 5.61% total carbohydrate did not significantly affect larval mortality when compared to the control diet. However, diets containing 9.45% and 15% total carbohydrate concentrations significantly increased larval mortality 64% and 96.1%, respectively, when compared to the control. Also in experiment I, terpenes alone (78.9% morality) and terpenes in combination with 9.45% and 15% total carbohydrates significantly increased larval mortality (97.2% and 100%, respectively) when compared to mortality on the control diet (44%). To determine which carbohydrate was causing the adverse effect, 6% glucose, 6% fructose, and 6% galactose were placed individually and in combination with terpenes in diets in experiment II. The 6% galactose diet significantly increased larval mortality and reduced growth rate when compared to the control, glucose, and fructose diets. Glucose resulted in 16% less larval mortality, significantly enhanced female larval growth rate and pupal weight, but did not affect male larval growth rate and pupal weight, when compared to the control. Fructose resulted in a significant decrease in larval mortality and a general trend of enhanced female and male larval growth rate and pupal weight. Larval mortality on terpenes alone was not significantly different from the control, but terpenes with 6% galactose increased larval mortality and decreased female and male growth rate and pupal weight significantly when compared to glucose-terpene and fructose-terpene diets. No significant interactions were found between carbohydrates and terpenes in either experiment.
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  • 71
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    Journal of chemical ecology 20 (1994), S. 579-591 
    ISSN: 1573-1561
    Keywords: Aldehydes ; formulation ; 10,12-hexadecadienal ; 10,12,14-hexadecatrienal ; moth ; Manduca sexta ; Lepidoptera ; Sphingidae ; tobacco hornworm ; trapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In field experiments traps were baited with live females or with a two-, four-, or eight-component blend of the 16-carbon aldehydes previously identified as components of the sex pheromone emitted by femaleManduca sexta moths. The blends were formulated on rubber septa. Traps baited with a blend of all eight aldehydes captured moreM. sexta males than any other treatment. Septa loaded with 600 μg of the eight-component blend were attractive to males for about seven days in the field. Septa loaded with the eight-component blend and stored in a refrigerator at 4°C for a year released the conjugated diene and triene aldehydes at the same rate as freshly prepared septa and were equally attractive in the field.
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  • 72
    ISSN: 1573-1561
    Keywords: Sex pheromone ; attraction inhibitor ; behavioral antagonist ; mating disruption ; air permeation ; field EAG ; Cydia nigricana ; Tortricidae ; Lepidoptera ; pea moth ; (E,E)-8,10-dodecadien-1-yl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Synthetic sex pheromone of the pea mothCydia nigricana, (E,E)-8,10-dodecadien-1-yl acetate (E8,E10–12: Ac), was applied in polyethylene dispensers at a rate of 30 g/ha and 600 dispensers/ha in a 0.6-ha pea field. The release rate ofE8,E10–12: Ac was 140 mg/ha/day after six days, and 82 mg/ha/day after 20 days. Aerial concentrations ofE8,E10–12: Ac, as measured by a portable EAG apparatus, ranged from 2 ± 2 to 7 ± 3 ng/m3. The antennal signal was high and rather constant within pea canopy, but was lower and fluctuated strongly above canopy. Initially, 〉99% isomerically pureE8,E10–12: Ac was released, and male moths were attracted to dispensers. After nine days, isomeric blend composition had equilibrated to approx. 92%E8,E10–12: Ac and 8% of the inhibitory isomersE,Z-,Z,E-, andZ8,Z10–12: Ac. Males were then repelled from the pheromone-permeated field. Traps baited with 100 µgE8,E10–12: Ac caught 258 ± 133C. nigricana males/trap in the control, but no males at all in the disruption field.
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  • 73
    ISSN: 1573-1561
    Keywords: Centaurea maculosa ; sesquiterpene lactone ; cnicin ; host-plant selection ; oviposition behavior ; antifeedant ; attractant ; Spodoptera littoralis ; Agapeta zoegana ; Stenodes straminea ; Pterolonche inspersa ; Lepidoptera ; Noctuidae ; Cochylidae ; Pterolonchidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The sesquiterpene lactone cnicin was extracted fromCentaurea maculosa andCentaurea vallesiaca. We examined its effects on the ovipositional response and larval development of generalist and specialist insect herbivores associated withC. maculosa. For the oviposition trials, three plant species (C. maculosa, Achillea millefolium, andCichorium intybus), half of which were sprayed with 3% of cnicin, were exposed to the specialist mothsStenodes straminea, Agapeta zoegana, andPterolonche inspersa in field cages. All three species significantly preferredC. maculosa to other plants andP. inspersa significantly preferred cnicin-sprayed plants to untreated plants for oviposition. Tested over all species, cnicin significantly increased the number of eggs laid on a given plant. A larval diet test examined the toxicity of cnicin for larvae of the generalist noctuid mothSpodoptera littoralis. Cnicin concentrations of 3% and 6% were lethal and 1% and 0.5% seriously inhibited growth and development. The larvae of theC. maculosa specialistStenodes straminea survived at 6% cnicin, but none of the pupae hatched.Agapeta zoegana was able to survive at 1% and 3% cnicin. Both specialists had difficulties with the artificial diet, but weight increase and survival was not further reduced when cnicin was present compared with on the control diet. In conclusion, cnicin influenced host recognition by the specialist species, and larvae of the generalist did not survive on natural levels of cnicin. Growth and survival of the specialist were not influenced by cnicin but were considerably hampered on artificial diet.
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  • 74
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    Journal of chemical ecology 20 (1994), S. 1025-1037 
    ISSN: 1573-1561
    Keywords: Pieris rapae ; Pieris napi oleracea ; Lepidoptera ; Pieridae ; oviposition ; stimulants ; glucosinolates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The relative activities of 10 glucosinolates in stimulating oviposition byP. rapae andP. napi oleracea were compared under the same conditions. When tested at the same concentration, the structurally different glucosinolates stimulated both butterfly species to widely varying degrees. In most cases,P. rapae was more sensitive to aromatic and indole glucosinolates than to aliphatic representatives. This species responded even less to alkyl thio and sulfinyl glucosinolates. However,P. napi oleracea responded strongly to these aliphatic and sulfur-containing members of the group, and the relative activities of aromatic and aliphatic glucosinolates did not show a clear pattern for this species.P. napi oleracea was much more sensitive to low concentrations of sinigrin than wasP. rapae. The threshold concentration for response ofP. napi oleracea to sinigrin was 10−8 M, which was 100 times lower than forP. rapae, butP. rapae was more sensitive thanP. napi oleracea to changes in glucosinolate concentrations. For bothPieris species, an optimal concentration was reached, above which the response remained constant or tended to decrease.
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  • 75
    ISSN: 1573-1561
    Keywords: Cotesia rubecula ; Hymenoptera ; Braconidae ; Lepidoptera ; Pieridae ; Plutellidae ; Pieris rapae ; Brassica oleracea ; Plutella xylostella ; Phaselus vulgaris ; Geranium molle ; tritrophic interactions ; infochemicals ; volatiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The role of airborne infochemicals in host selection by the parasitoidCotesia rubecula (Marshal) (Hymenoptera: Braconidae) was examined in a wind tunnel. To elucidate the role of volatile chemicals in attractingC. rubecula to cabbage infested by the host [Pieris rapae L. (Lepidoptera: Pieridae)], the potential sources of volatiles related toP. rapae infestation on cabbage were tested individually. The responses of females to nonhost plant species, bean and geranium, as well as to frass of a nonhost lepidopteran were also examined.C. rubecula was attracted to cabbage previously infested byP. rapae and to frass and regurgitate ofP. rapae. No attraction was observed to larvae ofP. rapae alone. Females were also attracted to mechanically damaged cabbage, cabbage previously infested byPlutella xylostella L. (Lepidoptera: Plutellidae) (a nonhost lepidopteran herbivore), and cabbage previously infested by snails (a nonhost, noninsect herbivore). Intact cabbage, bean, and geranium plants elicited no attraction. A low frequency of attraction was observed to mechanically damaged bean and geranium. Attraction was also observed to frass ofP. xylostella. Volatiles from cabbage related to damage, and volatiles from frass and regurgitate of the host seem to play an important role in guidingC. rubecula to plants infested by its host.
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  • 76
    ISSN: 1573-1561
    Keywords: Cotesia rubecula ; Hymenoptera ; Braconidae ; Lepidoptera ; Pieridae ; Plutellidae ; Pieris rapae ; Plutella xylostella ; Helix aspera ; Brassica oleracea ; Phaseolus vulgaris ; tritrophic interactions ; synomones ; infochemicals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The responses of the parasitoidCotesia rubecula to differently damaged cabbages were recorded during a series of choice tests. To determine if flyingC. rubecula can discriminate differences in the blend of volatiles emitted by cabbages damaged by different causes and how plant volatiles released from a distant source affect the searching behavior ofC. rubecula once searching on a plant, wasps were presented with a choice of plants located one behind the other and separated by a distance of 15 cm. The sources of damage were: cabbage damaged by the host (Pieris rapae), by a nonhost lepidopteran herbivore (Plutella xylostella), by a nonhost, noninsect herbivore (snail), and by mechanical means. The results showed that the site of first landing and the time spent searching on the leaves was influenced by the type of damage inflicted on plants. Wasps preferred to land on cabbages damaged by host and nonhost species of Lepidoptera over those damaged by snails and mechanical means. No preference was observed for first landing between cabbages damaged by the two species of Lepidoptera or between cabbages damaged by snails and mechanical means. Cabbage damaged byP. rapae was searched most intensively, followed by cabbage damaged byP. xylostella, cabbage damaged by snails, and cabbage damaged by mechanical means.C. rubecula differentiates between the volatile blends emitted by differently damaged cabbages, and it is attracted to volatiles related to recent lepidopteran damage. Wasps searched longer on freshly damaged than on leaves with older damage.
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  • 77
    ISSN: 1573-1561
    Keywords: Galleria mellonella ; Apis mellifera ; Pyralidae ; Apidae ; Lepidoptera ; Hymenoptera ; greater wax moth ; honeybee ; propolis ; plant resins ; phenolics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Bee propolis is a sticky amalgamation of plant resins collected by honeybees (Apis mellifera L.) and used in the hive for filling cracks and repairing combs. Propolis contains a diversity of compounds of plant origin, and is reported to have medicinal, antimicrobial, insecticidal, and phytotoxic properties. We examined the physical and chemical composition of North American samples of bee propolis from several sites in North America and tested for bioactivity against larvae of the greater wax moth (Galleria mellonella L.), a common apiary pest. The amount of methanol-extractable resin in samples from Ohio and Georgia ranged from 24% to 79% by weight. Propolis collected from hives in Ohio was more chemically diverse (over 30 compounds detected by paper chromatography) than material from south Georgia (fewer than 10 major compounds) and contained a lower proportion of methanol-insoluble beeswax. The paper chromatographic surveys revealed little variation in the chemical profile of specific hives over a six-month period and no differences between propolis from adjacent hives. Four flavonoids were identified from propolis collected in Ohio: kaempferol, galangin, 3,3′-dimethoxyquercetin and 3-methoxykaempferol. When mixed into artificial diet, fractionated propolis reduced larval growth of the greater wax moth, but not dramatically. An array of phenolics reported from propolis (caffeic acid, chrysin, ferulic acid, galangin, kaempferol, and quercetin) were bioassayed individually for effects on larvae, but none reduced larval growth at the concentrations tested, suggesting that wax moths are tolerant of some phenolics in their diet.
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  • 78
    ISSN: 1573-1561
    Keywords: Pieris rapae ; Lepidoptera ; Pieridae ; Plutellidae ; Plutella xylostella ; Cotesia rubecula ; Hymenoptera ; Braconidae ; Brassica oleracea capitata ; infochemicals ; plant volatiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract To elucidate the identity of the volatile compounds that could be involved in the searching behavior of the parasitoidCotesia rubecula Marshall (Hymenoptera: Braconidae), the volatiles released by cabbage and frass of Lepidoptera feeding on cabbage were collected and analyzed using a gas chromatograph-mass spectrometer. The volatiles emitted by intact cabbage were α-pinene, β-pinene, myrcene, 1,8-cineole,n-hexyl acetate,cis-3-hexen-1-yl acetate, and dimethyl trisulfide. Mechanical damage on an intact plant induced the release of two more compounds,trans-2-hexenal and 1-methoxy-3-methylene-2-pentanone. Current feeding by larvae ofPieris rapae L. (Pieridae) induced the plant to release all the compounds released after mechanical damage and additionally 4-methyl-3-pentenal and allyl isothiocyanate. Current feeding by larvae ofPlutella xylostella L. (Plutellidae) induced the plant to release all the compounds present after mechanical damage and additionally allyl isothiocyanate. The volatiles emitted after feeding by the lepidopterans had ceased were the same as those emitted by cabbage damaged by mechanical means. The blend of volatiles emitted by frass was comprised of plant chemicals, mainly sulfur compounds. Frass ofP. rapae emitted allyl isothiocyanate, methyl isothiocyanate, methyl propyl sulfide, dimethyl trisulfide,S-methyl methane thiosulfinate, 4-methyl-3-pentenal,trans-2-hexenal, and 2,3-dihydro-4-methyl furan. Frass ofP. xylostella emitted only dimethyl trisulfide andS-methyl methane thiosulfinate. The blend of volatiles emitted by frass is herbivore-species specific.
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  • 79
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    Journal of chemical ecology 20 (1994), S. 1985-2001 
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Lasiocampidae ; Malacosoma disstria ; Orgyia leucostigma ; larva ; tannin ; tannic acid ; peritrophic membrane ; oxidation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Final-instarMalacosoma disstria fed artificial diets containing tannic acid develop lethal pupal deformities. We examined some of the factors potentially underlying tannin sensitivity in this species, including the permeability of the peritrophic envelope to tannic acid and the chemical fate of tannic acid in the gut. Tannic acid does not penetrate the peritrophic envelope ofM. disstria, demonstrating that the containment of tannic acid within the endoperitrophic space is not sufficient to protect an insect herbivore from the adverse effects of ingested tannins. Ingested tannic acid undergoes extensive chemical modification in the midgut. Only 19–21 % of the high molecular weight components of the tannic acid ingested was recovered in the frass. Of two possible chemical fates of ingested tannic acid, oxidation is the predominant chemical transformation, whereas little hydrolysis occurs. Measurements of gut redox parameters showed that conditions in the midgut favor the oxidation of phenols. However, similar conditions occur in the midguts ofOrgyia leucostigma, in which no oxidation occurs. Therefore, oxidizing gut redox conditions do not necessarily lead to polyphenol oxidation in lepidopteran larvae. We conclude that the sensitivity ofM. disstria to ingested tannins is a consequence of their oxidation in the midgut.
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  • 80
    ISSN: 1573-1561
    Keywords: Apiaceae ; Peucedanum ; Lepidoptera ; Noctuidae ; Spodoptera littoralis ; HPLC ; preparative isolation ; furocoumarins ; furanocoumarins ; pyranocoumarins ; growth inhibition ; dietary utilization ; plant chemical diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Peucedanum arenarium Waldst. & Kit.,P. austriacum (Jacq.) Koch,P. coriaceum Reichenb.,P. longifolium Waldst. & Kit,P. officinale L.,P. oreoselinum (L.) Moench,P. ostruthium L., andP. palustre (L.) Moench accumulate different structural types of coumarins including simple coumarins, linear furanocoumarins, linear dihydropyranocoumarins, angular dihydrofuranocoumarins and angular dihydropyranocoumarins. Linear furanocoumarins, known for various biological activities, include some well-known antifeedants, such as bergapten, isopimpinellin, and xanthotoxin. The aim of this investigation was to screen the diverse coumarins fromPeucedanum for insecticidal activity. LC was used to analyze and isolate coumarins for the bioassays. A growth inhibition bioassay with 17 derivatives, comprising all structural types fromPeucedanum, carried out withSpodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae) as test organism, indicated the majority of the linear furanocoumarins and the angular dihydrofuranocoumarin athamantin as active compounds. Oxygenation of the prenyl residue of linear furanocoumarins decreased activity. Further formation of an ester with angelic acid even resulted in complete inactivity. Five active linear furanocoumarins, bergapten, isopimpinellin, xanthotoxin, isoimperatorin, and imperatorin, and two linear furanocoumarins with a substituted furan ring, peucedanin and 8-methoxypeucedanin, were compared in a dietary utilization bioassay. Relative growth rate (RGR) and relative consumption rate (RCR) divided the tested coumarins in three groups of similar activity. Isopimpinellin and peucedanin slightly decreased RGR and RCR of the treated larvae, and xanthotoxin, isoimperatorin, and 8-methoxypeucedanin heavily decreased RGR and RCR. Bergapten and imperatorin differed by the lowest RGR values and rather high RCR values. The effects caused by these two coumarins indicate specific postingestive toxicity. The results obtained in this study add to the reputation of coumarins to be an effective chemical defense, postulating that chemical diversity is a necessary trait for well-defended plants.
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  • 81
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Pyralidae ; Chilo partellus ; spotted stem borer ; female sex pheromone ; (Z)-11-hexadecenal ; (Z)-11-hexadecen-1-ol ; multicomponent pheromones ; signal integrity ; dispensing technique ; trap efficiency ; proximity effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The effect of proximity of the release points of the two pheromone components (Z)-11-hexadecenal and (Z)-11-hexadecen-1-ol of the spotted stem borer,Chilo partellus (Lepidoptera: Pyralidae) on behavior of the males and on trapping efficiency was investigated. Separating the dispensers of the two components in the trap by a mere 3 cm resulted in a threefold decrease in trap performance, compared to very close release of the components. The result is attributed to possible distortion of the pheromone signal, resulting in confused behavior ofC. partellus males in the vicinity of the trap. The ethological and practical implications of the phenomenon are discussed.
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  • 82
    ISSN: 1573-1561
    Keywords: Polyommatus icarus ; Lepidoptera ; Lycaenidae ; Coronilla varia ; Medicago sativa ; Fabaceae ; flavonoids ; sequestration ; plant-insect interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Larvae of the lycaenid butterflyPolyommatus icarus were reared on inflorescences ofCoronilla varia andMedicago sativa, which are rich in flavonoids. Twelve different flavonoids (five compounds from the former and nine from the latter), including aglycones andO-glycosides of kaempferol, quercetin, and myricetin were isolated and identified by spectroscopic means. NMR and MS data for the new acylated glycoside kaempferol 3-O-6″-(3-hydroxy-3-methylglutaroyl)-β-d-glucopyranoside are reported. Comparative HPLC analysis of the respective host plants and of larvae, pupae, and imagines ofP. icarus indicated selective uptake and accumulation of kaempferol vs. quercetin and myricetin derivatives. The latter were excreted largely unchanged through the feces. Irrespective of the larval host plant kaempferol 3-O-glucoside was found as the major flavonoid in larvae, pupae, and imagines ofP. icarus, accounting for approximately 83–92% of all soluble flavonoids in adult butterflies. Within the imagines, approximately 80% of all flavonoids are stored in the wings (especially in the orange submarginal lunules), whereas the remaining 20% reside in the bodies. Feeding experiments with artificial diet demonstrated that the insects are able to form kaempferol 3-O-glucoside by glucosylation of dietary kaempferol. Possible functions of the sequestered flavonoids, especially for mate recognition ofP. icarus, are discussed.
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  • 83
    ISSN: 1573-1561
    Keywords: Pheromone titer, (Z)- and (E)-11-tetradecenyl acetate ; (Z-11-tetradecenol ; age ; time of day ; constant and fluctuating temperature ; Choristoneura rosaceana ; oblique-banded leafroller ; Lepidoptera ; Tortricidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Under a 16:8 hr light-dark photoperiod and 20†C constant temperature, the titers of (Z)-11-tetradecenyl acetate (Z11–14:Ac), (E)-11-tetradecenyl acetate (E11–14:Ac) and (Z)-11-tetradecenol (Z11–14:OH) produced by different-agedChoristoneura rosaceana virgin females varied significantly during the scotophase, with the maximum titer occurring before the onset of calling in day-0 and day-3 females, while in day-5 females the titer remained constant throughout the calling period. There was a significant decrease in the titer of all pheromone components with age, explaining the lesser attractiveness of day-5 females relative to day-0 and day-3 females observed in the field. Under a cold thermocycle simulating condition during the second flight period in the fall, the titers of all pheromone components did not vary with time of day. There was a significant decrease in the amount ofZ11–14:Ac with age but no changes occurred in the minor components. Furthermore, for any given age tested, the amount of each component produced during the period of maximal calling activity remained relatively similar at the two temperature regimes. However, as with the expression of calling behavior, pheromone production was initiated earlier at cooler than at warmer temperatures. At both temperature regimes, female age and time of day influenced the ratio of each pheromone component. These results are discussed in relation to the hypothesis that by calling earlier, less attractive older females may increase their probability of mating.
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  • 84
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    Journal of chemical ecology 20 (1994), S. 2733-2741 
    ISSN: 1573-1561
    Keywords: Sex pheromone ; pheromone components ratio ; (E,Z,Z)-4,7,10-tridecatrienyl acetate ; (E,Z)-4,7-tridecadienyl acetate ; rearing temperature ; Lepidoptera ; Gelechiidae ; potato tuberworm moth ; Phthorimaea operculella
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Rearing temperature modified the sex pheromone component ratio in the potato tuberworm moth,Phthorimaea operculella. This phenomenon seemed to be induced with ambient temperature by differences in timing and speed of biosynthesis between two pheromone components. (E,Z,Z)-4,7,10-Tridecatrienyl acetate (triene) was mainly synthesized during the pupal period, while most of (E,Z)-4,7-tridecadienyl acetate (diene) was synthesized during a short period just after emergence. Therefore, the ratio of triene in a newly emerging adult was relatively high at all temperatures although the amount of triene was relatively low at 35°C. On the other hand, the synthetic rate of accumulation of diene was clearly modified by ambient temperature. Biosynthesis of diene at 15°C was very low in the first two days and high in the third day. Consequently, a titer of the diene component at 15°C became approximately equivalent to that at 25°C one day later.
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  • 85
    ISSN: 1573-1561
    Keywords: Pyrrolizidine alkaloids ; Lepidoptera ; Nymphalidae ; Ithomiinae ; Mechanitis polymnia ; stereochemical inversion ; epimerization ; evolution of PA acquisition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Pyrrolizidine alkaloids (PAs), acquired by adults or larvae of Danainae and Ithomiinae butterflies and Arctiidae moths from plants, protect these lepidopterans against predators and are biosynthetic precursors of male sex pheromones. The investigation of PAs in many species of wild-caught adults of Ithomiinae showed lycopsamine (1) [(7R)-OH, (2′S)-OH, (3′S)-OH] as the main alkaloid. In incorporation experiments, PA-free (freshly emerged) adults of the ithomiineMechanitis polymnia were fed seven PAs: lycopsamine and four of its known natural stereoisomers—indicine (2) [(7R)-OH, (2′R)-OH, (3′S)-OH], intermedine (3) [(7R)-OH, (2′S)-OH, (3′R)-OH], rinderine (4) [(7S)-OH, (2′S)-OH, (3′R)-OH], and echinatine (5) [(7S)-OH, (2′S)-OH, (3′S)-OH], and two PAs without the 7-OH: supinine (6) [(2′S)-OH, (3′R)-OH] and amabiline (7) [(2′S)-OH, (3′S)-OH]. Males epimerized PAs 3, 4, and 5 mainly to lycopsamine (1). Females fed these same three PAs changed a smaller proportion to lycopsamine; their lesser capacity to modify PAs corresponds to their normal acquisition of already transformed PAs from males during mating rather than through visits of adults to plant sources of PAs. The alkaloids1 and2, both 7R and 3′S, were incorporated without or with minimum change by males and females. Feeding experiments with6 and7 (males only) showed an inversion at the 3′ center of6 and no change in7. The inversion from 7S to 7R (probably via oxyreduction) may be closely related to the evolution of acquisition of PAs by butterflies and moths. Two hypotheses are discussed: (1) The ancestral butterflies are probably adapted to tolerate, assimilate, and use (7R)-PAs (most common in plants; all widespread 1,2-unsaturated macrocyclic PA diesters show this configuration). The development of (7R)-PA receptors in the butterflies could lead to a specialization on this configuration in two ways: to help find PA plants and to utilize these components in sexual chemical communication. A later appearance of (7S)-PAs in plants could have selected an enzymatic system for the inversion of this chiral center in order to continue producing (7R)-PA-derived pheromones. (2) The inversion would be due to the evolution of a enzyme system specialized in the transport of (7R)-PAs to the integument; the failure of this system to carry (7S)-PAs led to an enzymatic system to invert them to transportable (7R)-PAs. In this case, the 7R configuration is an effect and not a cause of (7R)-PA-derived pheromones. In both hypotheses, the partial inversion of the 3′-asymmetric center, when the butterfly was fed intermedine (3), rinderine (4), and supinine (6), could be fortuitous due to the conformation of the molecule and/or the enzymatic system.
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  • 86
    ISSN: 1573-1561
    Keywords: Cabbage looper ; Trichoplusia ni ; Lepidoptera ; Noctuidae ; pheromone ; kairomone ; attractants ; host finding ; (S)-(+)-linalool
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Unmated female or male cabbage looper moths,Trichoplusia ni (Hübner), were attracted more often in a flight tunnel to a cage with moths of the opposite sex and a bouquet of cotton foliage. Increased sexual attractiveness of females with plants may be a result of stimulation of pheromone release in response to plant odor, since more males were attracted when odor of cotton foliage was passed over females than when odor of females was passed over cotton foliage before venting into the flight tunnel. Increased sexual attractiveness of males with plants is due in part to host odor enhancement of female attraction to male pheromone, since more females were attracted to synthetic male pheromone (a blend of enantiomers of linalool and isomers of cresol) and a cotton leaf extract than were attracted to male pheromone alone. A short synthesis procedure was developed for (S)-(+)-linalool, the major component of the male sex pheromone, isolated from hair pencils, used in these tests.
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  • 87
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    Journal of chemical ecology 20 (1994), S. 2705-2719 
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Pyralidae ; Ostrinia nubilalis ; 11-tetradecenyl acetate ; (Z)-14-fluoro-11-tetradecenyl acetate ; (Z)-14,14,14,-trifluoro-11-tetradecenyl acetate ; pheromone analogs ; fluorinated analogs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Partially fluorinated analogs of the European corn borer (Ostrinia nubilalis) female sex pheromone, 11-tetradecenyl acetate (97:3Z:E), having mono- and trifluorsubstitutions at the terminal carbon of the pheromone chain, mimicked the biological activity of the pheromone, while analogs with fluorine at either side of the double bond and a pentafluoro analog were essentially inactive. Comparison of the pheromonal activity of these analogs with the previously reported activity of similarly fluorinated pheromones in five other species of moths revealed an unpredictable relationship between fluorine substitution pattern and pheromone-mimicking activity. Fluorine substitution patterns that rendered pheromonal analogs biologically inactive in the European corn borer had no detrimental influence upon pheromonal activity in other species and the converse was also true. This is evidence that the relative importance of electronic qualities of sites within a pheromone molecule differ from species to species. Furthermore, it indicates that the biochemical components (pheromone receptor proteins, binding proteins, and enzymes) that make up moth olfactory chemosensory systems must also vary structurally from species to species, despite the fact that they are involved in olfactory sensing of compounds having very similar chemical structure.
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  • 88
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Psychidae ; Oiketicus kirbyi ; bagworm ; sex pheromone ; pheromone chirality ; 1-methylbutyl octanoate ; 1-methylbutyl non-anoate ; 1-methylbutyl decanoate ; 1-methylbutyl dodecanoate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Gas chromatographic-electroantennographic detection (GC-EAD) analyses of pheromone extract of female bagworms,Oiketicus kirbyi (Guilding), revealed five EAD-active compounds. Retention index calculations, GC-mass spectrometry in both full-scan and selected-ion monitoring modes and GC-EAD analyses of authentic standards identified the compounds as 1-methylbutyl octanoate (MBO), 1-methylbutyl nonanoate (MBN), 1-methylbutyl decanoate (MBD), 1-methylpentyl decanoate (MPD), and 1-methylbutyl dodecanoate (MBDD). Of these five chiral esters, MBD was most abundant in extracts and elicited the strongest antennal response. In field experiments in Costa Rica, (R)-MBD attractedO. kirbyi males, whereas (S)-MBD in combination with (R)-MBD inhibited response.R but notS enantiomers of MBO, MBN, and MBDD strongly synergized attraction to (R)-MBD. (S)-MBO and (S)-MBDD were inactive, whereas (S)-MBN was inhibitory. (R)-, (S)- and racemic MPD were inactive. Blends of (R)-MBD in ternary combination with either (R)-MBO and (R)-MBN or (R)-MBN and (R)-MBDD were as attractive as the five-ester blend. Five- and four-ester blends were equally attractive, suggesting redundancy of pheromone components for attraction of males. The multiple sex pheromone component blend of chiral esters inO. kirbyi may have evolved to maintain species-specific communication in bagworm communities of tropical Americas.
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  • 89
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    Journal of chemical ecology 20 (1994), S. 651-666 
    ISSN: 1573-1561
    Keywords: Helicoverpa zea ; Lepidoptera ; Noctuidae ; lipoxygenase ; lipid peroxidation ; resistance ; herbivory ; soybean ; tomato ; cotton ; oxidative stress ; induced defense
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The potential role of the plant enzyme lipoxygenase in host resistance against the corn earwormHelicoverpa zea was examined. Lipoxygenase is present in most of the common host plants ofH. zea, with highest activity in the leguminous hosts such as soybean and redbean. Treatment of dietary proteins with linoleic acid and lipoxygenase significantly reduced the nutritive quality of soybean protein and soy foliar protein. Larval growth was reduced from 24 to 63% depending upon treatment. Feeding byH. zea on soybean plants caused damage-induced increases in foliar lipoxygenase and lipid peroxidation products. Larvae feeding on previously wounded plant tissue demonstrated decreased growth rates compared to larvae feeding on unwounded tissue. Midgut epithelium from larvae feeding on wounded tissues showed evidence of oxidative damage as indicated by significant increases in lipid peroxidation products and losses in free primary amines. The potential role of oxidative and nutritional stress as a plant defensive response to herbivory is discussed.
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  • 90
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    Journal of chemical ecology 20 (1994), S. 1571-1581 
    ISSN: 1573-1561
    Keywords: Male sex pheromone ; n-alkanes ; leek moth ; Lepidoptera ; Acrolepiidae ; hair-pencils ; gas chromatography-mass spectrometry ; gas chromatography-FT-IR ; female arrestant ; acceptance posture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Seven compounds that do not exist in the extracts from legs of males have been isolated in the hair-pencil extracts of maleAcrolepiopsis assectella. By combining techniques of GC-MS and GC-FT-IR, six of these compounds have been identified. They are sixn-alkanes: hexadecane (C16), heptadecane (C17), octadecane (C18), nonadecane (C19), eicosane (C20), and heneicosane (C21). Twelven-alkanes of the homologous series, from the C14–C25 compounds were presented to virgin females, mated females, and males. At the end of the scotophase, four of then-alkanes (C16, C17, C19, C21) present in the hair-pencil extract induced the virgin females to adopt the acceptance posture after having induced the virgin females to remain stationary. The two othern-alkanes (C18 and C20) present in the extract have less effect on the females similar to then-alkanes not present in the males. The blends tested do not seem to indicate any synergy between the most active compounds. The threen-alkanes with an odd number of carbons and the C16 compound would thus be the principle components of the male pheromone ofA. assectella. As well as their role of female aphrodisiac, they tend to make males and fertilized females flee.
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  • 91
    ISSN: 1573-1561
    Keywords: 1,4-Naphthoquinone ; allelochemical toxicity ; detoxification ; plant-insect interaction ; Lepidoptera ; Saturniidae ; Actias luna ; Callosamia promethea ; juglone ; plumbagin ; menadione ; lawsone ; quinones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The preferred hosts of the saturniid mothActias luna include members of the Juglandaceae, whose foliage contain the toxin juglone (5-hydroxy-1,4-naphthoquinone). The performance ofActias luna andCallosamia promethea was compared when fourth-instar larvae of each were fed birch foliage, a mutually acceptable food plant, or birth supplemented with 0.05% (w/w) juglone.A. luna fed juglone exhibited no changes in developmental time or mortality compared to a diet without juglone. In contrast, juglone-supplemented diets, when fed toC. promethea, caused negative growth rate, and a 3.6-fold decrease in consumption rate. The performance ofA. luna also was compared on birch and walnut; larvae developed and grew more rapidly on an all-walnut vs. an all-birch diet. To examine the effect of 1,4-naphthoquinone structure onA. luna survival, first instars were fed on birch supplemented with varying concentrations of juglone (J), menadione (M), plumbagin (P), or lawsone (L). In diets supplemented at 0.05% (w/w), none of the compounds produced effects significantly different from controls. In diets supplemented at 0.5% (w/w), the treatments produced significant toxic effects in the order P〉M=L〉J for mortality, and P〉L〉M=J for increased developmental time. Late-instarA. luna are clearly resistant to juglone compared toC. promethea, and early-instarA. luna are resistant to several related 1,4-naphthoquinones. These results suggest a chemical basis for host choice among saturniids. In addition, the luna-walnut system may be a valuable model for studying quinone detoxication.
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  • 92
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Pieridae ; Pieris brassicae ; Host Marking Pheromone ; Cruciferae ; Brassica oleracea ; induction ; oviposition deterrency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Pieris brassicae L. butterflies secrete miriamides onto their eggs. These avenanthramide alkaloids are strong oviposition deterrents when sprayed onto a cabbage leaf. However, these compounds could not be detected in cabbage leaves from which egg batches had been removed two days after deposition and that still showed oviposition deterrency. It was concluded that the miriamides were not directly responsible for the avoidance by females of occupied leaves while searching for an oviposition site. Evidence was obtained that cabbage leaves themselves produce oviposition deterrents in response to egg batches. Fractions containing potent oviposition deterrents could be isolated from surface extracts of leaves from which previously laid egg batches had been removed. The term host marking pheromone that was used previously is not applicable in this case.
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  • 93
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    Entomologia experimentalis et applicata 69 (1993), S. 145-155 
    ISSN: 1570-7458
    Keywords: Lepidoptera ; Diptera ; crystal proteins ; Cyt proteins ; pathogenesis ; cytotoxicity ; solubility ; receptor binding ; pore formation ; synergism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 94
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    Entomologia experimentalis et applicata 68 (1993), S. 59-69 
    ISSN: 1570-7458
    Keywords: oviposition ; Pieris rapae ; Pieris napi oleracea ; Cruciferae ; Lepidoptera ; Pieridae ; stimulant ; deterrent ; insect-plant interaction ; host range
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Oviposition responses ofPieris rapae L. andP. napi oleracea Harris to nine crucifers, one Capparidaceae and one Tropaeolaceae were directly compared under controlled conditions. Chemical fractions from these plants were also tested on both insects for the presence of oviposition stimulants or deterrents. The results showed that plant chemistry is a key factor in differential selection of potential hosts by thesePieris species. Some plant species were equally acceptable to bothPieris species. However,P. rapae preferred cabbage over most test plants whereasP. napi oleracea strongly preferred plant species that were avoided byP. rapae. The observed preferences were explained in most cases by the presence of stimulants and deterrents in extracts of the plants. The twoPieris species have apparently evolved differential sensitivities to the chemical stimuli that trigger or deter oviposition. The balance of positively and negatively interpreted sensory signals evoked by plant chemicals obviously plays an important role in acceptance or rejection of a plant by both species. The role of specific glucosinolates and differing structure-activity relationships is suggested.
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  • 95
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    Entomologia experimentalis et applicata 67 (1993), S. 57-64 
    ISSN: 1570-7458
    Keywords: Cydia pomonella ; codling moth ; mating disruption ; (E,E)-8,10-dodecadien-1-ol ; pheromones ; apple ; Lepidoptera ; Tortricidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polyethylene dispensers (Shin Etsu) containing 172 ml of the sex pheromone, (E, E)-8,10-dodecadien-1-ol (63%), dodecenol (31%) and tetradecenol (6%), of codling moth (CM),Cydia pomonella (L.), were placed in apple orchards in Virginia. Two blocks of about 2 ha each were treated in 1989, and three in 1990. Dispensers were placed in trees at a density of 1000/ha shortly after apple bloom. Male orientation to pheromone traps was almost totally disrupted (a few males were captured at high population densities). In 1989, the Daleville pheromone-treated block had 0.9% and 0.8% CM-injured fruit in the center and edge, respectively; 0% and 39.5% injured fruit were found in the conventional control and abandoned blocks, respectively. The Criglersville orchard (‘organically’ managed, with high CM density) CM harvest injury was 16.0%, 16.5%, 34.5%, and 26.5% in the pheromone-treated center and edge, organic control and abandoned blocks, respectively. In 1990, the Daleville CM harvest injury was 4.7%, 7.3%, 1.1%, 0.3% and 58%, in the pheromone-treated center and edge, control center and edge, and abandoned blocks, respectively (possible reasons for the high injury in this block are discussed). Harvest injury in the Fincastle pheromone-treated and control blocks were 0.7% and 0%, respectively. The Criglersville orchard yielded 17%, 19% and 20% CM-injured fruit at harvest in the pheromone-treated, organic control and abandoned blocks, respectively. Pheromone release rate was calculated as 37 mg/ha/h in 1989.
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  • 96
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    Entomologia experimentalis et applicata 67 (1993), S. 47-56 
    ISSN: 1570-7458
    Keywords: Platynota flavedana ; variegated leafroller ; Platynota ideausalis ; tufted apple bud moth ; Argyrotaenia velutinana ; redbanded leafroller ; mating disruption ; pheromones ; apple ; Lepidoptera ; Tortricidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mating disruption for control of variegated leafroller (VLR),Platynota flavedana (Clemens), tufted apple bud moth (TBM),P. ideausalis Walker, and redbanded leafroller (RBL),Argyrotaenia velutinana (Walker), was studied in Virginia apple orchards in 1989 and 1990. In 1989, each dispenser (1000/ha) contained 190 mg of 67.2%E11–14:Ac, 28.8%Z11–14:Ac, 1.4%E11–14:OH, 0.6%Z11–14:OH, and 2%Z9–12:Ac (a putative generic leafroller disruption blend). Trap captures of VLR, TBM and RBL were reduced by 97%, 51% and 55%, respectively. Average leafroller injury in the interior and edge of the pheromone block was 3.8% and 2.7%, respectively. The conventional control and abandoned blocks had 0.05 and 27.5% injury, respectively. Dispensers containingE11–14:OH (70%) andZ11–14:OH (30%) (close to the natural blend of VLR), more effectively disrupted orientation to pheromone traps by bothPlatynota spp. than did the generic blend. In 1990, dispensers containing 150 mg ofE11–14:OH (70%) andZ11–14:OH (1000/ha) were placed in two 2-ha blocks and one 4-ha block. One pheromonetreated block was sprayed in August with phosmet for codling moth, not timed for leafrollers. Trap captures of VLR and TBM were reduced by almost 100% and 69%, respectively. RBL captures were not reduced by VLR pheromone permeation. Injury fromPlatynota spp. in pheromone block middles and edges ranged from 0.3–1.7% and 0.3–2.3%, respectively. Injury in conventional blocks ranged from 0–1.1%. RBL injury in pheromone block middles ranged from 0–6.1%, and in edges, 1.7–4.8%. Injury in control blocks ranged from 0–1.1%. Combined leafroller injury in an abandoned block was 18% (s.e. 0.3). Release rates averaged 30 and 32 mg/ha/h for the VLR and generic pheromone dispensers, respectively.
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  • 97
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    Entomologia experimentalis et applicata 67 (1993), S. 79-85 
    ISSN: 1570-7458
    Keywords: Insecta ; Lepidoptera ; Noctuidae ; host-finding ; behavior ; oviposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mated femaleTrichoplusia ni (Hubner) moths, when presented a choice of either undamaged cotton plants,Gossypium hirsutum L., or damaged plants (cut leaves or feedingT. ni larvae) in a flight tunnel, were most often attracted first to the damaged plants. However, these same moths oviposited primarily on the undamaged plants. In a similar test with cabbage plants,Brassica oleracea L., the presence of conspecific larvae decreased both attraction and oviposition. Cuts to cabbage leaves had no significant effect on attraction or oviposition. When presented one plant at a time, percentages of cabbage looper moths attracted were not affected by the presence of larvae on either cabbage or cotton plants, or by cuts to cabbage plant leaves. Percentages of moths attracted were, however, higher using cotton plants with cut leaves. The results suggest an important role for damage induced plant volatiles in host location as well as host acceptance byT. ni.
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  • 98
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    Entomologia experimentalis et applicata 68 (1993), S. 117-126 
    ISSN: 1570-7458
    Keywords: insect-plant interaction ; Lepidoptera ; Plutellidae ; crucifer ; sexual maturation ; reproductive diapause ; kairomone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The influence of the presence of seedlings of a host plant, Oriental mustard,Brassica juncea (L.) Coss., on the calling behaviour and egg maturation in the diamondback moth,Plutella xylostella (L.), was investigated. In the presence of plants, females began calling at a younger age, began calling earlier in the night, and spent more time calling. Females which were kept with plants until after their first night of calling and then were isolated from plants, subsequently called less, at a level not significantly different from females which had never been exposed to plants. Of all females, both with and without plants, which were dissected at the end of the first night of calling (n=150), 93% contained mature eggs. Heavier females were significantly more likely to have mature eggs at adult emergence, to begin calling at a younger age and to spend more time calling on their first night of calling. The number of developed eggs in calling females was not significantly different in the presence or absence of host plants on the first night of calling. However, by the second night, females in the presence of plants contained significantly more eggs, indicating that the presence of plants accelerated egg maturation, both before and after the onset of calling.
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  • 99
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    Journal of insect behavior 6 (1993), S. 771-781 
    ISSN: 1572-8889
    Keywords: foraging behavior ; caterpillar-feeding damage ; paper wasps ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Experiments were performed to determine the effect of caterpillar feeding damage on wasp foraging behavior and to determine the relative importance of visual and olfactory plant cues for foraging wasps. In an experiment using caterpillar-damaged leaves, wasps took significantly more larvae from the previously damaged plants compared to the controls in the experiments with tobacco plants, but wasps did not distinguish between damaged and control plants in the experiments with tomato plants. Another experiment indicated that wasps use a combination of visual and olfactory cues of plant damage in their search for prey rather than just visual or olfactory cues alone. Furthermore, these results suggest that leaf shape may affect wasp detection of caterpillar feeding damage and thus detection of prey.
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  • 100
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    Entomologia experimentalis et applicata 67 (1993), S. 199-207 
    ISSN: 1570-7458
    Keywords: Helicoverpa zea ; Lepidoptera ; Noctuidae ; precocene II ; growth inhibition ; excretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Newly-ecdysed last instar larvae ofH. zea grouped into 100-, 200-, 300-, or 400-mg categories were fed diet containing precocene II or given precocene II topically on the abdomen. The time for larvae to reach a maximal weight, time to pupation, growth rate, and the amount of precocene II excreted were calculated. Younger larvae of lower weights, which were fed or topically treated with precocene II required more time to reach their maximal weight, had a lower maximal weight, a lower growth rate, and required more time to pupate than control larvae. Older larvae represented by the largest weight category were less sensitive to precocene II, had a shorter delay in reaching maximal weight, and a shorter delay in the time to pupation than control larvae; larvae in the largest weight category that were fed precocene II also had smaller decreases in the growth rate. Growth rate declines for larvae given topical doses of precocene II, however, were largest for the oldest larvae. All larvae given a single topical dose excreted precocene II for several days and were most efficient at eliminating smaller doses; larger, older larvae excreted more precocene II than smaller, younger larvae. Age-dependent responses to precocene II indicate that growth and metabolic processes, as well as xenobiotic metabolism, change in last instar larvae.
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