Publication Date:
1981-07-10
Description:
Applications of the new fracture-labeling techniques for the observation of cytochemical labels on platinum-carbon replicas are described. Frozen cells, embedded in a cross-linked protein matrix, and frozen tissues are fractured with a scalpel under liquid nitrogen, thawed, labeled, dehydrated by the critical point drying method, and replicated. This method allows direct, high-resolution, two-dimensional chemical and immunological characterization of the cellular membranes in situ, as well as detection of sites within cross-fractured cytoplasm and extracellular matrix.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉da Silva, P P -- Kachar, B -- Torrisi, M R -- Brown, C -- Parkison, C -- New York, N.Y. -- Science. 1981 Jul 10;213(4504):230-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7244630" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigens/analysis
;
Cells/*ultrastructure
;
Cytoplasmic Granules/ultrastructure
;
Endoplasmic Reticulum/ultrastructure
;
Erythrocytes/ultrastructure
;
Freeze Fracturing/*methods
;
Humans
;
Indicators and Reagents
;
Intracellular Membranes/ultrastructure
;
Leukocytes/ultrastructure
;
Microscopy, Electron/methods
;
Pancreas/ultrastructure
;
Pituitary Gland, Anterior/ultrastructure
;
Platinum
;
Rats
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink