Publication Date:
2001-08-18
Description:
We characterized an activation mechanism of the human LTRPC2 protein, a member of the transient receptor potential family of ion channels, and demonstrated that LTRPC2 mediates Ca2+ influx into immunocytes. Intracellular pyrimidine nucleotides, adenosine 5'-diphosphoribose (ADPR), and nicotinamide adenine dinucleotide (NAD), directly activated LTRPC2, which functioned as a Ca2+-permeable nonselective cation channel and enabled Ca2+ influx into cells. This activation was suppressed by intracellular adenosine triphosphate. These results reveal that ADPR and NAD act as intracellular messengers and may have an important role in Ca2+ influx by activating LTRPC2 in immunocytes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sano, Y -- Inamura, K -- Miyake, A -- Mochizuki, S -- Yokoi, H -- Matsushime, H -- Furuichi, K -- New York, N.Y. -- Science. 2001 Aug 17;293(5533):1327-30.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Molecular Medicine Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd., 21 Miyukigaoka, Tsukuba, Ibaraki 305-8585, Japan. sano.yorikata@yamanouchi.co.jp〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11509734" target="_blank"〉PubMed〈/a〉
Keywords:
ADP-ribosyl Cyclase
;
Adenosine Diphosphate Ribose/metabolism/pharmacology
;
Adenosine Triphosphate/metabolism/pharmacology
;
*Antigens, CD
;
Antigens, CD38
;
Antigens, Differentiation/metabolism
;
Apoptosis
;
Calcium/*metabolism
;
Calcium Channels/*metabolism
;
Cell Line
;
Eosinophils/*metabolism
;
Humans
;
*Ion Channels
;
Jurkat Cells
;
Membrane Glycoproteins
;
Membrane Potentials
;
*Membrane Proteins
;
Monocytes/*metabolism
;
NAD/metabolism/pharmacology
;
NAD+ Nucleosidase/metabolism
;
Patch-Clamp Techniques
;
Reverse Transcriptase Polymerase Chain Reaction
;
T-Lymphocytes/*metabolism
;
TRPM Cation Channels
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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