Publication Date:
2002-01-19
Description:
Application of nerve growth factor (NGF) covalently cross-linked to beads increased the phosphorylation of TrkA and Akt, but not of mitogen-activated protein kinase, in cultured rat sympathetic neurons. NGF beads or iodine-125-labeled NGF beads supplied to distal axons resulted in the survival of over 80% of the neurons for 30 hours, with little or no retrograde transport of iodine-125-labeled NGF; whereas application of free iodine-125-labeled NGF (0.5 nanograms per milliliter) produced 20-fold more retrograde transport, but only 29% of the neurons survived. Thus, in contrast to widely accepted theory, a neuronal survival signal can reach the cell bodies unaccompanied by the NGF that initiated it.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉MacInnis, Bronwyn L -- Campenot, Robert B -- New York, N.Y. -- Science. 2002 Feb 22;295(5559):1536-9. Epub 2002 Jan 17.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell Biology, 6-14 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada, T6G 2H7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11799202" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Axons/*metabolism
;
Cell Survival/drug effects
;
Cells, Cultured
;
Chromones/pharmacology
;
Cross-Linking Reagents
;
Enzyme Inhibitors/pharmacology
;
Iodine Radioisotopes
;
Microspheres
;
Mitogen-Activated Protein Kinases/metabolism
;
Morpholines/pharmacology
;
Nerve Growth Factor/*metabolism/pharmacology
;
Neurons/metabolism/*physiology
;
Phosphatidylinositol 3-Kinases/antagonists & inhibitors/metabolism
;
Phosphorylation
;
Protein Transport
;
*Protein-Serine-Threonine Kinases
;
Proto-Oncogene Proteins/metabolism
;
Proto-Oncogene Proteins c-akt
;
Rats
;
Rats, Sprague-Dawley
;
Receptor, trkA/metabolism
;
Signal Transduction
;
Superior Cervical Ganglion
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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