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  • Temperature  (173)
  • Caspian Sea  (154)
  • Chemistry
  • LUNAR AND PLANETARY EXPLORATION
  • Inorganic Chemistry
  • Iranian Fisheries Science Research Institute  (322)
  • 1
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25112 | 18721 | 2018-08-23 05:48:23 | 25112 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Menmiopsis leidyi was entered into Caspian Sea from Black Sea and mass population constitutes in the southern Caspian Sea. In this surveys, the relationship between length and wet weight measured and length and weight followed by this equation: n=384, R^2=0.87 w=0.003L^9005 . The relationship of dry and wet weight calculated, in this surveys, the ratio of dry weight to wet weight was equal to 1.615% in 12 salinity. In vitro, the clearance rate at 12 and 27 C were 52.48 and 107.33 ml/h, respectively, daily ration at above temperatures were 1.98 and 16.65 respectively. Mnemiopsis leidyi can feed Acartia in accordance to 16.5 mg/1gbw (body weight) at 27 C in one day. The average digestive time in 12 C and 27 C were 77.50 min and 36 min respectively. The digestive value in 27 C was 2.5 fold than 12 C. The result indicated that clearance rate, daily ration and digest were increased in high temperature. Stomach content of Mnemiopsis leidyi was shown that this organism utilized from Acartia and Balanus, in addition, stomach content of kilka including two species of zooplankton from the southern Caspian Sea. Stomach contents of clupeonella cultiventris contain more than 54 percent of Acartia and its nauplii. This frequency observed in cluponella engrauliformis and clupeonella grimmi. On basis of zooplankton sampling in the sea, over 90 percentage of zooplankton populations contain Acartia.
    Keywords: Biology ; Iran ; Caspian Sea ; Menmiopsis leidyi ; Zooplankton ; Population ; Survey ; Feeding ; Cetenophora
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  • 2
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25322 | 18721 | 2018-09-12 09:50:04 | 25322 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Hamoun fish, Schizothorax zarudnyi, is an indigenous species of the eastern waters of Iran, which is exclusively found in this region. However recently drought occurrence in the Chahnimeh reservoirs (a semi natural water body) making them vulnerable to extinction. As an appropriate action to address this problem and according to the 3 side contract between Italian Government – UNDP - Islamic republic of Iran Government and the Italian government financial support to reduce poverty in the Province of Sistan-Baluchestan through the reinforcement and dissemination of aquaculture activities the project was developed at the Hamoun Research Unit by the Chahbahar Fisheries Research Center to record the breeding normative of 1 g weight larvae for restocking and other researching purposes. 331 broodstocks of the indigenous species Shizothorax zarudnyi weighing 800-2450 g were collected from the Chahnimeh reservoirs in early autumn, 2006. From 5/3/2007 (the project is supported and communicated on 2010) , Ovulation was stimulated with three stimulators; pituitary extract (3-6 mg kg-1 body weight), GnRH-A (20-30 mg kg-1 body weight) and anti dopamine (10-15 mg kg-1 body weight) that was given in 2-3 doses to breeders. Of 169 injected breeders , some were injected On mid March of 2007 (12-13 ºC water temperature) responded to the injection 25% ,while the rest were injected On April of 2008 (14-16 ºC water temperature) responded to the injection 65%. In the present project of 167 breeders 82 were female and 87 male. Totally 30 female breeders released their eggs in different stages. 20 female breeders released their eggs completely, 3 breeders released half of their eggs and 7 released 1/3 of their eggs. The male breeders just injected in the final dose of hormontrapy and all were ready for releasing sperms however the ovulation in female breeders occurs between 353-428 h ºC and after the final dose of injection. Ripe eggs were stripped from the females and fertilization was done by the dry method. Fertilized eggs were transferred to veis incubators and troughs. Incubation period for eggs differs and larvae hatch out after about 910 days at an average water temperature of 12.5 °C. Maintained at 13-14 °C, complete absorption of yolk sac in Shizothorax zarudnyi larvae occurred after 5-8 days. Larvae were fed with a mixture of powdered milk and egg yolk in this stage followed by decapsulated Artemia cysts and nauplii of Artemia and then on formulated starter diets used for carps. Because the ponds were not ready, larvae were maintained in troughs for about ten days before they were transferred to two 1200 m2 earthen ponds where they reached a body weight of about 1 g. They were then handed over to the Iranian Fisheries department in the region. Larvae were fed with the starter feed SFCO in the earthen ponds. About 350 thousand larvae were stocked in two earthen ponds. Based on the results of present study and other studies we may conclude that artificial breeding in Schizothorax can be successfully achieved at 14-16 ºC in flow through systems using hormone therapy (combination of GnRha and anti dopamine) and larvae could be easily cultured in earthen ponds. However this species exhibits lower growth rates as compared to carps its high expenses could have an important role in economical feasible.
    Keywords: Aquaculture ; Iran ; Sistan and Baluchestan province ; Chahnimeh reservoirs ; Chahbahar ; Hamoun fish ; Schizothorax zarudnyi ; Breeding ; Species ; Aquaculture ; Temperature ; Female ; Eggs ; Hormontrapy ; Sperm ; Ovulation ; Larvae ; Fisheries ; Earthen ponds
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  • 3
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25324 | 18721 | 2018-09-12 09:56:53 | 25324 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was conducted in order to optimizing of the biological condition and enhancing of the harvest efficiency for cultured shrimps of Guater site. Experimental design was consisted of three treatments and each treatment with three replications (totally 9 ponds with 600 m^2 area for each pond). After preparing and watering of ponds, the ponds was stocked with shrimp post larves in a density of 0.007±0.001 g (or 20 numbers per m^2) per m^2. After 35 days, the fingerlings of gray mullet were released to shrimp ponds in densities of 0/100 m^2 (T1/), 2/100 m^2 (T2) and 4/100 m^2 (T3). During experiment, the physicochemical parameters of water (temperature, O_2, pH, water transparency and salinity) were measured daily for two times (morning and afternoon). Also, the nitrate, nitrite, ammonia, phosphate and silicate concentrations and BOD5 as well as chlorophyll (a), phytoplanktons and zooplanktons were measured every 15 days one time. To determine the organic values of the bottom sediment of the ponds, monthly sampling was conducted. Health status of shrimps was investigated monthly too. Growth rate, mean weight, survival value, Food conversion ratios (FCR) and total shrimp harvest (Kg) were measured after 107 days rearing in ponds. ANOVA analysis showed significant differences between treatments. The highest (212 Kg, 3533 Kg; weight mean was18.4 g for each shrimp) and lowest (187 Kg, 3116 Kg; weight mean was16.23 g for each shrimp) of harvested shrimps were yielded in T3 and T1 respectively. There was not significant differences between treatments in terms of survival rate (P〉0.05). After 107 days rearing, the highest (1.27 0.2) and lowest (1.2 0.1) of FCR were observed in T_3 and T_1 respectively. Although, there were no significant differences between T_2 and T_3 in ammonia, O_2, Total organic material, pH and BOD5, but, T_2 and T_3 had significant differences with T_1 in these parameters. In this study, 27 genus of phytoplanktons belonged to Diatoms (Bacillariophyceae), blue-green algal (Cyanophyceae) and dinoflagellates (Dinophyceae) were identified. Diatoms with 20 genus had more abundance than dinoflagellates (6 genus) and blue-green algal (1 genus). Also, 7 orders of zooplanktons including copepod, mollusks, nauplius of crustacean and rhizopoda were identified. Health investigation of T1 (without shrimp) showed more pathogenic pollution (parasites and bacteria) than in treatments with shrimp. In this regard, among bacteria, the Vibrio genus (V.Alginoliticus and V.Fluvialis) had more abundance and among parasites zoothamnium sp and Epistylis sp were identified which more abundance was for zoothamnium sp. In conclusion, our results concluded that mixed culturing of white shrimp and grey mullet in optimum densities is possible and is caused more production of shrimp.
    Keywords: Aquaculture ; Iran ; Chabahar ; Guater ; Litopenaeus vannamei ; Mugil cephalus ; Mixed culturing ; Polyculture ; Earthen ponds ; Shrimp ; Fingerlings ; Gray mullet ; Temperature ; O2 ; pH ; Water transparency ; Salinity ; Chlorophyll a ; Phytoplanktons ; Zooplanktons ; ANOVA ; Diatoms ; Bacillariophyceae ; Blue-green algae ; Cyanophyceae ; Copepod ; Investigation
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  • 4
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25327 | 18721 | 2018-09-12 09:54:28 | 25327 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Present project was conducted in shrimp farm located in east of Chabahar in Sistan and Balouchestan province.Twentieth sampling stations at three locations (i.e. 2 stations in supply channel,13 stations in drainage channel and 5 stations in Gwatar Gulf) were selected to determine physical and chemical factors such as; temperature, salinity, dissolved oxygen, pH, nitrate, nitrite, ammonia, phosphate and biological parameters for example Chlorophyll a, phytoplankton and zooplankton. Sampling was conducted once a month from March 2003 until January 2004. The results revealed different bioenvironmental area in the three locations (i.e. supply channel, drainage channel and Gwatar Gulf) over the study period. Concentration of salinity ,pH , nitatr ,nitrite ,ammonia and chlorophyll a in drainage channel were more than supply channel and Gawtar Gulf. While concentation dissolved oxygen and phosphate were in drainage channel less than Gawtar Gulf.Also, sediment with small grain size (clay and silt) caused uptake phosphate and amount phosphate decreased in drainage channel. Considering with lack of environment standards in Iran shrimp effluent, data compared with others countries standard. Results showed that with considerable farm area, value of temperature, salinity, dissolved oxygen and pH were in permit range in Gwatar Gulf while concentation nitrate, phosphate and chlorophyll a were sometimes more than permit range that could cause plankton bloom. Also, Gwatar Gulf were influence monsoon. Nitate, phosphate, chlorophyll a and phytoplankton increased in gwatar Gulf.Regards with that postmonsoon was simultaneus with shrimp harvest.Therefore, increase some parameters could be caused from shrimp effluent or postmonsoon. Thirty four phytoplankton genus were identified including diatoma, dinoflagellate, cyanophyta and chlorophyta. In each area maxiumum percentage of phytoplankton were belonged to diatoma (63%) in supply channel, cyanophyta (51.7%) in drainage channel and dinoflagellate (94.5%) in Gwatar Gulf.Amount and range chemical and physical factors and plankton composition were different in drainage channel, supply channel and Gwatar Gulf. Cyanophyta could grow more in drainage channel.Percentage of aboundance and diversity of phytoplankton changed in 2002 inrelation to years ago( before shrimp culture, 2000 year and 2001 year) specially in Gwatar Gulf. Mainly percentage of zooplankton were copepoda and thintinida respectively.Diversity zooplankton decreased ratio to years ago. In drainage channel, abundance total of phytoplankton and zooplankton were more than Gwatar Gulf and supply channel.
    Keywords: Aquaculture ; Iran ; Sistan and Blouchestan ; Gwatar ; Chabahar ; Environmental effect ; Shrimp culture ; Survey ; Sampling ; Temperature ; Salinity ; Dissolved oxygen ; pH ; Chlorophyll a ; Phytoplankton ; Zooplankton ; Diatoma ; Cyanophyta ; Chlorophyta ; Copepoda
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  • 5
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25333 | 18721 | 2018-09-12 09:59:44 | 25333 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study has been carried out in waters of Bushehr province in the Persian Gulf covering Nayband bay to Bahregansar between 26° 45 N to 30° 00 N and 49° 45 E to 52° 45 E, 15 main and 5 sub- stations, by R/V Ferdows 1 in winter 2004, summer 2004 and winter 2005. The physico - chemical parameters, nutrients (silicate, phosphate, nitrate and nitrite), phyto, zooplanktons, sediments and macrobenthoses were collected from water layers and sea bottom, and then analysed. The parameters were compared and evaluated with the results of the previous work, conducted in the same area. Termocline, halocline, oxycline and picnocline layers were formed in summer. These clins disappeared due to water column mixing in winter. The values of temperature, conductivity and pH in summer were higher than those of in winter, while the salinity and density values of surface and subsurface layers in winter were higher than those of in summer. The temperature and conductivity have been decreased from surface to depth, from coastal rigions to offshore and from southern waters to northwern waters, while salinity, density and turbidity have been increased. The dissolved oxygen is decreased by increasing the values of temperature, salinity and depth and it is observed that D.O. has increased in correlation with chlorophyl a in mean and subsurface layers. The pH was rather decreased from surface to depth while turbidity was increased. Orthosilicate (Si_SiO44), nitrate (N_NO3), and nitrite (N_NO2 ) concentration have increased from surface to depth while orthophostate (P_PO43) decreased. Among the phytoplanktons, Dinophycea had most abundance in winter 2004 and Diatoms had most abundance, variety and distribution in summer 2004 and winter 2005. Among the zooplanktons, Copeopda had most abundance, variety and distribution. The intensity of phytoplanktons in summer was more than those in winter; it decreased from surface to depth and from coastal rigions to offshore, at first increased and then gradually decreases. Intensity of zooplanktons in summer was more than those in winter and decreased from surface layers to depth and from coastal waters to offshore. The abundance of benthoses in summer was more than that in winter while their biomass in winter was more than that in summer. The abundance decreased from southern to northwern waters, but the rate of decrease in biomass was more than that of abundance. The mainly texture of sediments was loamy or muddy and the grain size in southeastern and northwestern areas were coarser than those in middle area. Compared with the previous study, the trend of physico chemical, silicate, nitrate and nitrite were the same but in spite of the past, the values of pH in summer were more than those in winter and also phosphate concentration had been decreased frome surface layer to depth. The overall trends of phyto and zooplanktons densities are almost similar to previous observation, but cyanophyceae significantly decreased, in comparison with spring and summer abundance. It is also observed that the zooplanktons average density has been in reverse order compared to previous observations. Average concentration of phosphate in the entire region was seven times more than that of the former study and its maximum value has rised more than 26 times in the petroleum and gas industries areas. On the other hand, the abundance and diversity of phyto and zooplanktons and also the ratio of biomass to abundance of benthoses were decreased.
    Keywords: Ecology ; Iran ; Persian Gulf ; Bushehr Region ; Nayband ; Bahregansar ; Hydrology ; Hydrobiology ; Nutrients ; Phytoplankton ; Zooplankton ; Sediments ; Macrobenthose ; Temperature ; Dissolved oxygen ; Chlorophyll a ; pH ; Diatoms ; Copeopda ; Depth ; Monitoring
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  • 6
    Publication Date: 2021-07-16
    Description: The culture of three species green tiger prown (Penaeus semisulcatus) ,white leg shrimp (Litopenaeus vannamei) and Indian white shrimp (Fenneropenaeus indicus) were grown in 7 ponds (0.4 and 0.25 hectare) for 110 days at a stocking density of 25 shrimp/m2 on Hele Research Station. The average of temperature and salinity was 30 degrees centigrade and 46-48 ppt .At the end of the culture mean weights of L.vannamei 18.68±2.6 was higer than the P.semisucatus 7.77±1.49 and F.indicus 14.66 ±2.1 also results demonstrated that there was a significant relation (P〈0.05) between mean weight. Final yield were 3114, 1983 and 848 Kg for L.vannamei, F.indicus and P.semisucatus.The FCR was better for L.vannamei (1.28) than F.indicus (1.48) and P.semisucatus (2.15).
    Keywords: Aquaculture ; Iran ; L.vanname ; F.indicus ; P.semisucatus ; Mean Weight ; Culture ; Native species ; Green tiger prown ; Penaeus semisulcatus ; Shrimp ; Indian white shrimp ; Temperature ; Salinity ; FCR
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  • 7
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25342 | 18721 | 2018-09-13 12:35:36 | 25342 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was conducted during April to June 2007 .Pomferet brooders were captured by gill net (5 m Height, 300 m length, 7 cm size) during tidal time with 20-40 minutes interval. Khor Mermis, Khor Patil and Khor Mavi were subjected for broodstock captures. Totally 237 brooders were captured (126 male and 111 female) and were kept in 300 L. tanks. Semi dried method were used for breeding with 0.6 % fertility solution. Hatching were placed during 16-18 hoers. Fish larvae were kept in 4 T. tanks and were fed by fresh rotifer .temperature and salinity was kept 31-28 degree centigrade and 45-47 ppt. respectively .results shows successful breeding of pomferet in 2007 .egg diameter was 1.1 mm and hatching were completed during 16-18 hours at 31-29 degree centigrade. After 31 days length of larvae was 40 mm. while for 7 and 15 days length of larvae was 4.5 and 8.4 mm respectively.
    Keywords: Aquaculture ; Iran ; Feasibility ; Pomferet ; Pampus argentus ; Breeding ; Marine ; Gill net ; Height ; Length ; Size ; Broodstock ; Fertility ; Larvae ; Temperature ; Salinity
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  • 8
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25352 | 18721 | 2018-09-14 06:57:48 | 25352 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: 15000 juvenile Indian majour carps (Labeo Rohita,Cirhinus merigala, Catla catla) imported (200 ) and transferred to the earthen ponds in Astaneh Fisheries Research Station , In order to assess the viability of rearing under the climatic condition of north part of Iran and also achieve some growth parameters from the larval stage to market size.The fry with 300 mg weight, were released in to 12 ponds. Specimens were separated to tree experimental categories: 1= (30%indian carp and 70% chinees carp) , 2= (50%indian carp and 50% chinees carp) , 3= (70%indian carp and 30% chinees carp) .single chinees carp production were used as control . fishes were fed with artificial food and also with the natural products of the ponds which were enriched with organic fertilizers, after being equally stocked in the ponds .During the experiment, oxygen level ,PH, turbidity, and temperature were measured. Monthly fry samples were taken, for weight and length biometrical analysis and also determination of the weight gain percentage, average daily growth rate, specific growth rate. Results have indicated adaptations and considerable growth in this species, with an increase from the initial weight of 300 mg to an average of 13.5±1.6 mg in 50 days period until the end of November for fingerlings. Also the average weight of growing phase (second year of production ) was 647g for Labeo Rohita ,420 g. for Cirhinus merigala and 734g for Catla catla. The special growth rate (SGR)in Roho was 4.51 %.and 3.9 % for merigal and6.2 % for Catla while for Chinees carps SGR was 4.9, 6.7, 7.6 and 5.8 for silver carp,grass carp, common carp and big head respectively. Results revealed that the Indian majour carps compatibility with the condition in Gilan province was succesfull and the combination of species and optimization of their compatibility were remind for futhre studies.
    Keywords: Aquaculture ; Iran ; Guilan Province ; Astaneh ; Indian carp ; Growth ; Labeo Rohita ; Cirhinus merigala ; Catla catla ; Polyculture ; Juvenile ; Earthen ponds ; Fisheries ; Rearing ; Larval stage ; Fry ; Specimens ; Artificial food ; Fertilizers ; pH ; Turbidity ; Temperature
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  • 9
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25362 | 18721 | 2018-09-14 07:41:38 | 25362 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Golestan coast at south of Caspian Sea is the most important aquasystems, because of its relation to lagoon, bay and fresh water rivers and of its role in life cycle of bony fishes. The study of heavy metals, as a kind of chemical and environmental pollution is an essential program; because, they accumulated in the aquatic environment and human food chain that cause ecological damages. In this study, we determine the concentration of four heavy metals (Cd, Cr, Zn, Pb) in some most consumed fishes, such as Liza auratus, Cyprinus carpio and Rutilus frisii kutum in winter, spring and summer seasons locating at 11 sites (west and east coast, Gomishan Lagoon and Gorgan Bay). The fishes which were caught from the mentioned areas after biometry, immediately freezed and transferred to laboratory for further analyses. Then, water and sediment samples were acid digested and assessed for determination of Cadmium, Lead, Zinc and Chromium levels using atomic absorption spectrometer (AAS). The results showed that the highest concentration of Lead (214.83 ppb) was related to Gomishan Lagoon and the lowest Chromium level (28.83 ppb) was observed in the water specimens sampled from east coast in winter. Otherwise, the highest concentration of Zinc (300.7 ppb) was found in sediment specimens sampled from Gomishan in summer and the lowest concentration of Cadmium (12.7 ppb) in Gorgan Bay in summer. According to the results, the concentrations of heavy metals in liver tissues excised from fishes were higher than that in muscle tissues. Also, there was significant differences were observed between Chromium concentrations in muscle tissue of Cyprinus carpio with sex but there was not for other fish. In this investigation, we found a positive liner relationship between accumulation lead with age for Rutilus frisii kutum. On the other hand, no significant relationship was found between accumulations of heavy metals with age for other fish. Comparison of the resulted mean concentrations of heavy metals with international standards (WHO, UK (MAFF), NHMRC), showed lower concentrations in liver and muscle tissues of sampled fishes.
    Keywords: Pollution ; Iran ; Caspian Sea ; Golestan Province ; Gomishan Lagoon ; Gorgan Bay ; Heavy metals ; Liza auratus ; Cyprinus carpio ; Rutilus frisii kutum ; Sediment ; Aquasystem ; Bony fishes ; Pollution ; Aquatic ; Specimens ; Muscle tissue ; Sample
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  • 10
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25373 | 18721 | 2018-09-14 07:27:49 | 25373 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The project of "Assessment of different methods of water treatment on decreasing pathogenic bacteria in shrimp hatcheries" achieved by mean of reviewing the effectiveness of various methods on the water treatment to reduce bacterial load of sea water. The project performed in Bandargah region of Bushehr province in order to checking the effect of different methods on the sea water and bacterial population. This project has been done over a year. In this study a total of 138 samples was collected during seven times and was evaluated as well as 7 treatments. In this study, the comparison of the effect of each factors (sand filter, chlorine, ultra violet ray, chlorine combined with ultra violet ray) was analyzed on the sea water by means of disinfection. During this study, other environmental factors such as water temperature, dissolved oxygen, pH and salinity was measured till we can ensure that they don’t have any negative impact on the treatments. Results showed that the density of total bacteria in sea water (Control) was averagely 5187 CFU/ml, this was done while the total density of bacteria in the sea water, reservation pool sample, sand filter water, water that irradiated with ultraviolet rays, chlorinated water and the water that was influenced by both chlorine and ultra violet ray was respectively 5187 2137, 4449 2042, 2782 1335, 1164 692, 143 104, 87 76 CFU/ml. Therefore, reservation pool, sand filter, ultra violet ray, chlorine, chlorine-ultra violet ray each of them was reduce respectively 14, 46, 77, 97, 98 percent of the density of total bacteria or in other words they reduced 0.06, 0.27, 0.65, 1.56, 1.77 log of the total population of bacteria in water. Moreover, the results from the total count of vibrios showed that reservation pool, sand filter, ultra violet ray, chlorine, chlorine-ultra violet ray each of them was reduce respectively 17, 47, 74, 98, 99 percent of the density of total vibrios or in other words they reduced respectively 0.08, 0.27, 0.59, 1.66, and 1.95 log of the total population of vibrios in sea water. Also the bacteria that isolated from each treatment were identified with the use of biochemical methods that totally were includes a wide range of bacteria, the identified vibrios were: Vibrio alginolyticus, V. costicola, V. fischeri, V. fluvialis I, V. fluvialis II, V. harveyi, V. natriegens, V. nigripulchritudo, V. parahaemolyticus, V. plagius I, V. vulnificus, V. anguilarum I, V. campbellii, V. nereis, V. proteolyticus, V. splendidus, and Unknown V. spp. Other identified bacteria belonging to the following genus: Acinetobacter, Aeromonas, Alcaligenes, Bacillus, Borkholderia, Eschricia, Enteroacter, Flavobacterium, Micococcus, Proteus, Pseudomonas, Serratia, Staphaphylococcus, Streptococcus, and Unknown Bacteria spp. By comparing the results, we concluded that the best result was related to treatment that the sea water had been disinfected after the combined effects of chlorine and ultraviolet ray. It was determined that this process in compared with other treatments, had been reduced the total number of bacteria, the total number of Vibrios, the number of sucrose fermentative vibrios, and the number of non-sucrose fermentative vibrios respectively 98, 99, 98, 99 percent. In other words, the number of bacteria was reduced respectively 1.77, 1.94, 1.82, 2.23 log. So in this study, the applied method was introduced as the most effective way of disinfecting sea water. Meanwhile, none of pathogenic bacteria for shrimp including Vibrio harveyi was isolated by using this treatment. Comparing the results showed that the combined effects of chlorine and ultraviolet radiation is the most effective applied method for disinfecting sea water.
    Keywords: Aquaculture ; Iran ; Bushehr province ; Bandargah region ; Shrimp ; Water treatment ; Sea Dissolved oxygenwater ; Bacteria ; Hatchery ; Assessment ; Population ; Temperature
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  • 11
    Publication Date: 2021-07-16
    Description: Fish soup powder is produced from three kinds of fish in Chabahar catched in Oman sea white flesh of many low value fish like threadfin bream, perches etc. can be used to prepare instant fish soup powder. This product has a high consumer acceptability in many countries in this research project we used 4 low value fishes with local name of Sarm, Chaman, Aroos and Talal belonging to families Carangidea, Lethrinidea, Drepanidae and Scombroidea. soup powder packed in laminated pouches and the shelf life of it is one year in ambient temperatures. The quality used to be good up to 6 month but after this time the quality decreases gradually. The experiment on fish soup showed that the percentage of protein was 32 percent, fat 11 percent and humidity 12 percent and ash 4 percent. The experiment conducted on fish soup powder was peroxide, total microbial count, organoleptic test for 6 month.
    Keywords: Fisheries ; Iran ; Oman sea ; Sistanblochestan province ; Chabahar ; Fish Soup Powder ; Species ; Fish ; Threadfin bream ; Carangidea ; Lethrinidea ; Drepanidae ; Scombroidea ; Temperature ; Protein ; Microbial count
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  • 12
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25423 | 18721 | 2018-09-23 16:29:10 | 25423 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The Blooming due to the some species of phytoplanktons especially Dynoflagellates has made some problems for water ecosystems and aquaculture. In this study, the density of phytoplanktons specially Cochlodinium sp. and also environmental factors such as temperature, pH, dissolved Oxygen, and transparency were recorded two weekly in 18 stations of Hormozgan province, Iran in order to monitoring of the possibility of phytoplankton blooming. During six months monitoring, the target phytoplankton, Cochlodinium sp was not observed in shrimp farms. But, other phytoplanktons and zooplanktons were observed as follow: 13 genus of phytoplankton and six genuses of zooplanktons has found in ponds, main water channel and sea. The diatoms with 10 genuses had the highest abundance and Dynoflagellates with 3 genuses had the lowest abundance and blue-green phytoplankton with one genus was in lowest group. Totally, diatoms with 77%, Dynoflagellate with 15% and blue-green alga with 8% abundance were the main populations of planktons in the studied area.
    Keywords: Aquaculture ; Iran ; Hormozgan Province ; Tiab ; Algae Cochlodinium sp ; Shrimp farms ; Species ; Phytoplanktons ; Dynoflagellates ; Aquaculture ; Temperature ; pH ; Dissolved oxygen ; Transparency ; Zooplanktons ; Blue-green algae
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  • 13
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25436 | 18721 | 2018-09-23 16:56:01 | 25436 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Harmful algal blooms resulting in red discoloration of coastal waters in the Persian Gulf, Iran were first observed in January 2007. The species responsible for the bloom, which was identified as Cochlodinium polykrikoides, coincided with massive aquatic organisms’ mortalities in the Persian Gulf. In order to provide optimum growth and bloom forming, C. polykrikoides cells were sampled during the bloom conditions in the coastal waters of Persian Gulf. After adaptation in filtered seawater, they isolated by positive phototropism characteristic of this species to light. They were grown in modified media culture at different salinity (30, 32 and 35ppt), temperature (20, 23, 26 and 28ºC) and intensity (35, 70 and 90 µmol m^-2 s^-1) with an initial cell density of 50 cell mL^−1. The results of the present study clearly showed that the highest alga biomass was obtained following culture by using A^2 medium under the 32ppt salinity, 26°C temperature, and under a 11h light:13h dark photoperiod regime at a light intensity of 90 µmol m^-2 s^-1 provided by cool white fluorescent tubes. Mean cell density of C. polykrikoides in a 60 liter tank for ten days reached to 32 × 10^6 cell L^−1. Moreover, individual C. polykrikoides chain with 18 cells was observed for the first time in cultures. Based on the results from the present study, as mentioned above, providing suitable media culture and physical condition (light intensity and temperature), bloom forming of C. polykrikoides start from day 8 to 10 and will be continued until day 24 t0 28. In the other hand, C. polykrikoide cells immediately crashed and destroyed.
    Keywords: Ecology ; Iran ; Persian Gulf ; Harmful algal bloom ; Cochlodinium polykrikoides ; Isolation ; Optimum growth ; Environmental parameters ; Phototropism characteristic ; Species ; Density ; C. polykrikoides ; Temperature ; Growth ; Biomass ; Bloom
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25444 | 18721 | 2018-09-23 17:01:05 | 25444 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The project of the Study on fishing and resource management of fisheries within Southern Caspian Sea was conducted on the base of an agreement made between fisheries organization (Shilat) and the Iranian fishery research organization (IFRO) signed in 2010. In this library-based study the current situation of bony fish fisheries and biology of these species has been surveyed in Iranian waters of the Caspian Sea . In this survey internal and external environmental factors of bony fishes activities were considered. Using SWOT analysis method, current situation of fisheries was investigated..One of the most important strategies for conserving anadromous species such as Caspian kutum , common carp, roach, bream and …. is rehabilitation of the natural spawning ground in rivers, coastal lagoon specially Anzali lagoon .By conducting this strategy not only restocks of bony fishes is done ,but also reduced the cost of artificial restocking programs and releasing fingerlings .In short term , it is nessesary to enhance the stocks of the species that have low catch is done by huge releasing of the fingerlings and study the qualification of the program.
    Keywords: Ecology ; Management ; Iran ; Caspian Sea ; Anzali lagoon ; Bony fishes ; Distribution ; Stocks ; Biology ; Fishing ; Species ; Caspian kutum ; Common carp ; Roach ; Bream ; Spawning ; Fingerlings
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  • 15
    Publication Date: 2021-07-16
    Description: Study on Viral Nervouse necrosis (isolation, characterisation and pathogenesis) in Golden grey mullet in the Caspian Sea and study of pathogenecity and possibility of transmission to the other fish species (Sturgeon fishes, Rutilus frisii kutum and reared Rainbow trout and Carp) Viral Nervous Necrosis (VNN) is a worldwide disease affecting several species of cultured marine fish. For the past two decades, betanodavirus infections that cause Viral Nervous Necrosis (VNN) have emerged as major constraints on the culture and sea ranching of marine fish in almost all parts of the world. More than forty species mainly of marine origin have been so far affected and this number is likely to rise in future following the introduction of new species and the increase of aquaculture trade. Unknown acute mortality occurred in wild golden grey mullet Lisa auratus and Liza saliens in Iranian waters of Caspian Sea in recent years. In order to isolation and confirmation of causative agents of golden grey mullet mortality in the Caspian Sea, a complementary research investigation project was designed in 2005 and approved immediately in Iranian Fisheries Research Organization (IFRO). Many diagnostic aspects such as Virology (Cell culture and Elctereone Microscopy), Hemathology, Bacteriology, Histopathology, Molecular biology (NestedRT-PCR), Heavy metals measuerment and Serology (IFAT and IHC) were employed in mentioned multidiciplinary project. About 322 moribund fish samples which revealed skin darkening, erratic swimming behavior such as spiral and belly-up at rest and high distention of swimming bladder. Suspected samples were collected from coastal capture sites in iranian north proviences in 2006 till 2009. Targets tissue such as brain and eye were removed in strile condition and then kept in -80oC frezzer for cell culture and Nested-RT-PCR. Other tissue samples from liver, kidney, intestine, stomach, gill, skin and muscle, gall bladder and gonads were taken and fixed in 10% buffer formalin and same parts fixed in glutaraldehyde 3% for histopathology, IHC and EM respectively. Cytopathic effect (CPE) was observed in those cell cultures just six days after inoculation with the dilutions of the tested 312 homogenate supernatants. CPE in monolayers of cells cultured (SSN-1 cell line) was characterised by thin or rounded, refractile, granular cells with vacuoles. Nine samples were positive in virology assay. Nested- RT-PCR was done on suspected tissue samples and supernatant of CPE positive samples and 21 tissue samples and all CPE positive samples were positive. IFAT was selected as a confirmatory method for identifying viral strains replicating on cell cultures and carried out with rabbit anti-betanodavirus serum on suspected tissue samples and some smears of CPE positive samples. Some bright points approved betanodavirus antigen and confirmed cell culture and Nested-RT-PCR findings. In fixed tissue samples widespread and massive vacuolation were observed in brain, spinal cord, retina and optical nerve. In order to confirmation of diagnostic findings , IHC was done with monoclonal antibody antibetanodavirus and some red-brown points were observed. Theses findings revealed expected viral antigens and confirmed previous results. Moreover, virus particles with 25-30 nm in diameter were visualized in infected brain and retina using positive staining in TEM. Also pathogenicity test was employed to confirm the obtained results. So Guppy fish Poecilia reticulata and sturgeon fry were used instead of the experimental host due to ease of handling and susceptibility. After 15 days post infection, guppy bathed in VNN-infected tissue culture with 104 TCID50 showed clinical signs similar to naturally infected Golden grey mullet, and the mortality rate reached up to 100% in 75 dpi. When target organs were examined by cell culture isolation, serology, and histopathology, all revealed the presence of virus in the Guppy. Suspected supernatant injected to sturgeon fry through intravitreous injection and widespread vacuolation were observed in brain and spinal cord buy IHC and Real time PCR were negative. In conclusion, with attntion to obtained results in this investigation such as ecological factors, clinical signs, histopathological, virological and bacteriological results, molecular analysis, (IHC, IFAT, PCR), TEM demonstration, serological and hematological findings, it could be confirmed that VNNV was the main causative agent for disease outbreak in Golden grey mullet in Southern coastline of Caspian Sea.
    Keywords: Aquaculture ; Health ; Iran ; Caspian Sea ; Viral nervous necrosis ; Golden grey mullet ; Liza aurata ; Liza saliens ; Histopathology ; Virology ; Bacteriology ; IHC ; IFAT ; PCR ; TEM ; Species ; Sturgeon ; Rutilus frisii kutum ; Rainbow trout ; Carp
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  • 16
    Publication Date: 2021-07-16
    Description: The survey effect of salinity, turbidity and dissolved oxygen performed on Rutilus frisii Kutum juveniles with aim, which was determined about survival rate and histological changes in gill and kidney tissues. Juveniles provided from the Rajai's fish propagation center and then examined in Caspian Sea Ecology Research Center laboratory. The juveniles were investigated on three weight group (200-〈400, 400-〈600, 600-〈1000 mg) and two dissolved oxygen levels (7.6 ± 0.19, 3.8 ± 0.15 mg/L)in fresh water and Caspian sea water(12.5 ppt). Also, six turbidity levels (50 FTU, 430FTU, 2600 FTU, 7800 FTU, 15600 FTU) were probed in freshwater during 168hr. Each treatment has three replications. The factorial test evaluated for survival rate in treatments. The results shown that there was a significant difference among treatments in the different levels of factors, (p〈0.05, Duncan test). The juveniles' survival rate has decreased than control treatment in brackish water and turbidity in freshwater. It was demonstrated that with increasing fish weight, therefore, the survival rate is increased. The lowest survival rates under salinity stress (〉75%) had shown in 400-〈600mg weight group with dissolve oxygen: 3.8±0.15SE mg/l. In addition, the lowest survival rates under turbidity stress (95.83%) had shown that in weight group less than 400 mg with turbidity 15600 FTU. Therefore, survival of juveniles was high. However, gill tissue of juveniles under turbidity and salinity stress became short (necrosis) and thick (edema and hyperplasia) in the end of experiments. The juveniles gill tissues were not changes in the river clear freshwater. Therefore, the factors of gill tissue deformation were salinity and suspended particles in the water. However, the structures of kidney in the different groups of juveniles were similar. Nevertheless, juveniles glomerular diameter increased with increasing weight (p〈0.05, Duncan test). Furthermore, there was a increasing the internal cavity of the proximal and distal tubules and decreasing of glomeruli in diameter in the transfer of juveniles from freshwater to brackish water. Although the survival rate of juveniles is acceptable in this study, but there was abnormal changes in the structure of them gills. It appears that this change will cause a disruption in trend of juvenile's growth.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Rutilus frisii Kutum ; Juvenile ; Salinity ; Turbidity ; Oxygen ; Survey ; Survival rate ; Gill tissue ; Freshwater ; Brackishwater
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  • 17
    Publication Date: 2021-07-16
    Description: Bony fish stocks are being legally utilized by 134 fishing cooperatives in the Iranian side of the Caspian Sea mainly through beach seine. This fishing method has been invariantly used over the past. Fifty years with actually no alteration or technical modifications in the fishing nets despite the substantial changes in the quality & quantity of bony fish resources. Based on the data obtained from the stock assessment project for bony fish, the amount of premature or young fish (substandard size) caught during the past decade has been far greater than those of authorized fish size and even the figures for certain species suggest heavy over fishing to such an extent that in the case of frisii kutum, there has been a reduction by 50% in the overall kutum landings. Thus in order to modify the technical design of the fishing with a view to alleviate the catch of non-standard fish and whereby prevent over fishing by beach seine, it seems imperative therefore to launch a biometric study on bony fish stocks particularly the two intended fish species namely Rutilus frisii kutum and mullet which would involve identification of their standard weight and length using pertinent mathematical equations as well as determining proper mesh size of the code-end and other parts of the nets & consequently the H. R, d/a coefficients. The operations related to project were carried out in the "Inland Aquaculture Research Institute" net mating plant and beach seine site in Anzali during 2005-7 which aimed at designing and constructing standardized beach seines and comparing the fishing performance & yields with those of ordinary beach seine nets under equal conditions with a ratio of 3:1. The results concerning the quality and quantity of fish caught during a whole fishing cycle were recorded in the related data forms. The statistical analysis consisting the comparison of the quality of fishing performance in both the experimental and control beach seine nets was made using T. student and excel, spss 12 software packages. The results indicated that the reduction in the amount of non-standard fish caught in standardized beach seine net (the experimental net) was meaningfully different from that of the ordinary (control) net showing the superior fishing performance of the former. The catch level of non-standard R.frisii kutum decreased from 67.1% in the control beach seine net down to 7.1% in the standardized beach seine net and these figures for mullet were 29.98% and 1.1% respectively. Based on the results obtained and considering the apparent superiority of the experimental net in catching greater number of mature fish and less number of premature or non-standard sized fish, the use of this modified beach seine net can be proposed to Iranian fisheries organization as a viable alternative to the traditional beach seine nets.
    Keywords: Fisheries ; Iran ; Caspian Sea ; Anzali ; Beach seine net ; Rutilus frisii kutum ; Mullet ; Standard sized fish ; Non-standard sized fish ; Bony fish ; Fishing ; Stock assessment ; R.frisii kutum
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  • 18
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25174 | 18721 | 2018-09-03 17:15:43 | 25174 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The population genetic structure of five Caspian Sea sturgeon species was investigated. Totally 1121 samples of caudal and unault's fin tissue of the sturgeons (Acipenser persicus, A. gueldenstaedtii, A. stellatus, A. nudiventris and Huso huso) were collected from the Volga River (Russia), Ural River (Kazakhstan), Kura River (Azerbaijan), Sepidrud River and the coastline of the south Caspian in the Iranian waters as well as from the sampling stations selected for the marine survey for sturgeon stock assessment in the Caspian Sea. All samples were stored in 96% ethyl alcohol and transferred to the genetic laboratory of the International Sturgeon Research Institute. Genomic DNA was extracted using phenol-chloroform method. The quality and quantity of DNA was assessed by Agarose gel (1%) electrophoresis and spectrophotometry. The population genetic structure of Ship and Persian sturgeon was studied using both PCR-RFLP (D-loop and ND5/6 gene) and microsatellite technique and that of H. huso, A. stellatus and A. persicus were studied using microsatellite technique. After amplification of genes using PCR, the RFLP technique was used to digest mtDNA using restriction enzyme. The PCR products were electrophoresed on 6% sequencing polyacrylamide gels followed by silver nitrate staining. Data for PCR-RFLP were analyzed using REAP program and those from microsatellite technique were analyzed using Gene Alex. Population genetic parameters including allele frequency, expected and observed heterozygosity, effective allele, Shannon's index were determined. Genetic identity and distance were calculated following Nei criteria and Hardy Weinberg equilibrium was tested based on X2 and analysis of molecular variance (AMOVA) using Reap and Gen Alex at 99% confidence limit. Phylogenetic relationship was determined and drawn using TFPGA program. The population genetic structure and genetic diversity of the 1121 sturgeon specimens were determined. Three independent populations were identified for Acipenser persicus (two populations in the south Caspian in the Iranian waters and one in the north Caspian). Three independent populations were identified for A. gueldenstaedtii (Volga, Ural and South Caspian populations) using the microsatellite technique. Population genetic structure using PCR-RFLP revealed no genetic differentiation among the A. gueldenstaedtii specimens studied from the different regions using ND5/6 gene, while two populations (Ural and south Caspian populations) were detected for this species with the same technique using D-loop genes. Four independent populations (Volga, Ural, Kura and Sepidrud populations) were reported for A. stellatus using the microsatellite technique and four more populations which most probably belong to the autumn and spring races of the above mentioned independent populations were identified for this species. The present study also identified two populations for H. huso; The North Caspian population (in Volga and Ural Rivers) and The South Caspian population (in Golestan and Guilan regions) which were significantly different from each other (P〈0.01). The genetic population structure of A. nudiventris was studied using the microsatellite and PCR-RFLP techniques which revealed two populations for this species one in the Ural River and the other in the Sepidrud River (South Caspian). Comparison of the ND5/6 and D-loop genes studies in Russian sturgeon revealed that the D-loop gene is better than the ND5/6 genes in population's differentiation and is therefore strongly recommended for population genetic studies on sturgeons in the Caspian Sea. Genetic diversity studied using microsatellite technique was higher and more accurate as compared to that using RFLP. Nevertheless the RFLP technique was able to introduce molecular markers for the population’s species pacific identification. On developing suitable primers these studies can be speeded up and the cost of such studies can be cut down. However the drawback in using microsatellite technique for population genetic studies is that it cannot introduce a molecular marker for the identification of populations. The present study was able to introduce molecular markers to differentiate the ship sturgeon population in the south Caspian from that in the Ural River using the PCR-RFLP technique Based on the results obtained it is strongly recommended that all activities related to restocking and rehabilitation of sturgeon stocks in Iran be conducted on the basis of genetic principles. Also serious and immediate measures should be taken for the restoration and conservation of rare population of native species of Iran particularly in the Sepidrud region using genetic markers before they are become extinct.
    Keywords: Biology ; Iran ; Caspian Sea ; Guilan Province ; Golestan province ; Sepidrud River ; Assessment ; Sturgeons ; Population ; Genetic ; PCR-RFLP ; Microsatellite ; Species ; Samples ; Tissue ; Acipenser persicus ; A. gueldenstaedtii ; A. stellatus ; A. nudiventris ; Huso huso ; Survey
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  • 19
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25176 | 18721 | 2018-09-03 17:05:47 | 25176 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In the early 1980s, an alien ctenophore Mnemiopsis leidyi already known as a gelatinous zooplankton was transported (likely via ballast waters) to the Caspian Sea from its introduced or native water of the Black Sea or western Atlantic which caused negative impacts. In this report, distribution of M. leidyi, planktonic and benthic organisms had been investigated in the Southern Caspian Sea (Iranian waters) in 6 transects consisted of Lisar, Anzali, Sefidroud, Nowshar, Babolsar and Amirabad at 26 stations during 2005 and 2006. As a whole, 1422 samples had been analyzed included 258, 346, 217, 117 and 484 belong to ctenophore, phytoplankton, zooplankton, benthos and physicochemical parameters, respectively. M. leidyi abundance and biomass were fluctuated between 284-2751 ind.m^-2 and 16.9- 390/9 g.m^-2 respectively in 2005 while maximum abundance and biomass were recorded in autumn and minimum in winter. In 2006, its mean abundance and biomass were 184-2150 ind.m^-2 and 9.1- 209/8 g.m^-2 respectively. M. leidyi maximum abundance and biomass were noted in summer and its minimum in spring and winter months. Overall, mean M. leidyi abundance and biomass were recorded in 20 m within 1202 ind.m^-2 and 139.5 g.m^-2 respectively. Maximum size of the ctenophore was recorded as 55 and 60 mm in 2005 to 2006 respectively, while less than 10 mm length frequency consisted 83.16 and 82.88 per cent of total population. Spatial_ temporal distribution of M. leidyi in different regions showed it was more abundant in west and east (714- 4494 ind.m^-2) ratio to central parts (13-1519 ind.m^-2) of the Southern Caspian Sea. Species composition of zooplankton had shown the negatively impacts of M. leidyi invasion in the period of sampling as the only 17 holozooplankton were determined with Rotatoria (7 species), Copepoda (4 species), Ciliophora (4 species) and cladocera (1 species). Zooplankton maximum abundance and biomass were recorded in February (3039742901 ind.m^-3 and 372.1575.4 mg.m^-3) respectively and its minimum were in July (12211601 ind.m^-3 and 6.9 7.7 mg.m^-3) respectively which was belonged to Rotatoria (76%) and Copepoda (10%). In this study, 21 species of benthic fauna were deter minted belong to Pseudocumidae (5 species), Gamaridae (4 species) Amphartidae (3 species), Oligochaeta, Balanidae, Xantidae, Nereidae, Scarbicularidae, Carididae, Mytilidae, Herpobdellidae and Chironomidae (each 1 species). Oligocheata was recorded with maximum abundance (943 2502 ind.m-2) and Bivalve consisted of higher biomass (68.7162.5 g.m^-2). From five phyla of phytoplankton, 172 species were determined of Chrysophyta (74 species), Cyanophyta (32 species), Chlorophyta (32 species), Pyrrophyta (24 species) and Euglenophyta (13 species), in which Chrysophyta and Pyrrophyta were noted as main groups with their maximum abundance (up to 65%) and biomass (93%). Maximum water temperature was recorded in August with 29.52.5 ֯C and minimum in February by 9.31.3 ֯C. Mean value of salinity was 12.010.90 ppt, secchi disk ranged between 0.2 to 7 m and oxygen demand varied from 5.95 to 10.54 mg. l^-1). Moreover, silicate concentration was recorded between 200 to 300μg.L^-1, Phosphate measured from 31-47 μg.L^-1, Ammonia varied in 10-29 μg.L^-1, Nitrite (0.6- 1.7 μg.L^-1), Nitrate (0.06-4.20 μg.L^-1, which mainly accumulated in coastal waters of the southern Caspian Sea.
    Keywords: Ecology ; Iran ; Caspian Sea ; Anzali ; Sefidroud ; Nowshar ; Babolsar ; Mnemiopsis leidyi ; M.leidyi ; Benthic ; Organisms ; Phytoplankton ; Zooplankton ; Benthos ; Gamaridae ; Amphartidae ; Oligochaeta ; Balanidae ; Xantidae ; Nereidae ; Scarbicularidae ; Carididae ; Mytilidae ; Herpobdellidae ; Distribution ; Species ; Biomass ; Abundance
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  • 20
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25179 | 18721 | 2018-09-03 17:23:17 | 25179 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study two species of algae, Sargassum glaucescens and Padina boergeseni that found plenteously in Persian Gulf and Bushehr coast, were collected and hot water extracts of them were lyophilized. F. indicus (11.32±1.20 g), after two weeks adaptation in Shoghab research station were immersed in seawater (39 ppt and 25±1 ○C) containing hot-water extract of each brown algae, S.glaucescens and P.boergeseni, at 100, 300 and 500 mg/l concentration, Survival rate and immunological parameters (total haemocyte count (THC), total plasma protein (TPP), Phagocytic activity, bacterial clearance efficiency and bactericidal activity) were examined. In addition effect of dietary administration of beta 1, 3 1, 6 glucan on prevention of White Spot Disease and immunological parameters of shrimp were investigated. According to results, immersion in seawater containing 300 and 500 mg/l concentration of algal hot-water extract after 2 and 3 hours or oral administration of beta 1,3 1,6 glucan at level of 10 g/kg diet for 14 days significantly enhanced THC, TPP, Phagocytic activity, bacterial clearance efficiency and bactericidal activity. Immersion in seawater containing 100, 300 and 500 mg/l hot-water extract of S.glaucescens after 3 hours, improved the survival rate of WSSV-infected F. indicus.
    Keywords: Biology ; Chemistry ; Iran ; Persian Gulf ; Bushehr Province ; Beta-glucan ; Sargassum ; Padina algae ; WSD ; Fenneropenaeus indicus ; Species ; Algae ; Brown Algae ; S.glaucescens ; P.boergeseni ; Survival rate ; White Spot Disease ; Shrimp
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  • 21
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25183 | 18721 | 2018-09-03 17:12:18 | 25183 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Possibility of rearing silver bream (Sparidentex hasta) in the earthen pond was experimented using 2 types of available artificial trout feed (FFT and GFT1). The experiment was carried out in 2 phases in Shahid-Kiani Extension Shrimp Farm in Chowibdeh Shrimp Site-Abadan Southern Iran, and earthen ponds with 0.25 ha. Area were used for both phases of the study. Juvenile fish of 3.2 g initial weight with the density of 9324 ind./ha. Were stocked for 196 days in the first phase. The initial weight, stocking density and rearing period of the fish in the second phase were 108.60 g, 9800 ind./ha. And 237 days respectively. Maximum amount of WG, FCR, PER and SVR of the fish in the first phase of the study were 100.12 g, more than 6, 0.38 and 55.32% respectively. WG and SR of the fish in the second phase respectively were 417 g and 20.74%. The Salinity of pond water was in the range between 9 and 22 ppt, and the water temperature was between 8.3 ֯C (mid December morning) and 34.8 ֯C (early Sep.....
    Keywords: Aquaculture ; Iran ; Abadan ; Chowibdeh ; Cultuation ; Silver Bream ; Sparidentexhasta ; Earthen Pond ; Artificial feed ; Rearing ; Shrimp ; Juvenile ; Weight ; Density ; Temperature
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  • 22
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25519 | 18721 | 2018-10-05 15:40:50 | 25519 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The Persian sturgeon (Acipenser persicus) is more abundant sturgeon species in the South Caspian Sea and consist the highest proportion of Iranian Caviar, meat as well as bringing maximum foreign currency income, however from systematic point of view and differentiation of this species from Russian sturgeon (Acipenser gueldenstadttii) a serious challenging issues remain, where some Russian scientist are believe that the Persian sturgeon is not as an valid species and consider it as a subspecies of Russian sturgeon. This research conducted with the objective of identification and introducing a molecular marker based on specific DNA for differentiation of two species of Persian sturgeon and Russian sturgeon via a proved molecular marker method. For this purposes 8 different molecular approaches such: Microsatellite, AFLP, RAPD, sequencing of Cytb, 16sDNA, ND5, Growth Hormone gene and finally Single Nucleotide Polymorphism (SNP) were investigated. Based on applied methodology, between 5 to 16 caudal fin tissues were sampled for each species from different region of the Caspian Sea, Sefiedrud River, Ural and Volga rivers. Following DNA extraction, its quality and quantity were determined and the PCR experiment has been conducted using 5-110 primers according to various methods and type of gene. The PCR products were electrophoresed on Polyacrilamid or agarose gels and followed by silver and Ethidium Bromide staining. In RAPD method, polymorphic DNA band was cut on the gel followed by purification and then the segments were cloned in vector in Top10 strain of E.coli, and then sequenced. Meanwhile for Growth Hormone gene in Persian and Russian sturgeon the MEGA 4, Gene runner software were used to design the appropriate primers for PCR amplification. The PCR products were cloned in PTZ57R/T vector and transformed in Top10 E.coli strain and sequenced finally. For all other genes, similar methods were applied for PCR amplification and its products were sequenced and statistical analysis as well as phylogenetical tree was performed. In Single Nucleotide Polymorphism (SNP) method, after genomic library construction, in total 14.4 billion nucleotides were sequenced and similarity/ differentiation analysis of two species were investigated using specific bioinformatic software. Results indicated that Microsatellite and AFLP methods showed high level of genetic variation both within and between species. The Cytb gene, when 4 sample sequences from each species were compared two species were differentiated, however when analysis repeated over 15 samples, the sequence comparison couldn't differentiate two above mentioned species. Full sequence comparison of 16sDNA and mtDNA-ND5 gene showed variation in some nucleotide in both species of Persian and Russian sturgeon but no significant. Results of sequences obtained from cloned segment with RAPD method and also specific primer design based on produced sequences could succeed to discover a variable DNA band that able to differentiate two species from each other. Results of the present study also showed that the growth hormone gene (GH) of Persian and Russian sturgeon consists of 645 nucleotide that translate to 214 Amino Acids. The sequence comparison indicated that the gene coding growth hormone in Persian and Russian sturgeon had the highest similarity with GH of Mammals (71%), Anguilaformes (63%) and less similarity with bony fish (37%). Phylogenetic analysis indicates that Persian and Russian sturgeon in compare to other organism are ancient species and this gene is originated from a common ancestor. At present study the most appropriate results obtained from Single Nucleotide Polymorphism (SNP) method by sequencing 14.4 billion nucleotide from genome of two species of Persian and Russian sturgeon from North and the South Caspian Sea could prove that the Persian sturgeon is a valid and independent specie. This excellent results is the biggest scientific achievement for differentiation of two highly commercial important sturgeon species in the Caspian Sea in last two decades.
    Keywords: Biology ; Iran ; Caspian Sea ; 16S rDNA ; Acipenser persicus ; Persian sturgeon ; Acipenser gueldenstadttii ; DNA ; Russian sturgeon ; Species differentiation ; Molecular Marker ; SNP
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  • 23
    Publication Date: 2021-07-16
    Description: Exploitation of bony fishes resources started on 12 October 2005 and finished on 9 April 2006 in 2005-2006. 142 beach seines caught about 14333.4 tons of bony fishes by 47101 hauling. The total catch was estimated 21844.7 tones (Includes illegal fishery). Kutum, mullets and common carp comprised more than 97 percent of total catch. Length classes 39-40, 31-32 and 40-41 cm predominated for kutum, golden grey mullet and common carp, respectively. Age groups 3 to 5 year comprised 84.2, 74.8 and 83.7 percent for Kutum, golden grey mullet and common carp, respectively and age group 4 year was dominant with 42.0, 35.9 and 43.0 percent, respectively. K value and L_∞ calculated 0.26 (/year) and 58.3 cm for Kutum and 0.15 (/year) and 61.5 cm for golden grey mullet, respectively. The total biomass and MSY estimated about 24733.7 and 8550.4 tones for kutum and about 16948.0 and 4999.0 tones for golden grey mullet, respectively. Exploitation rate (E) calculated 0.71 and 0.70 for Kutum and golden grey mullet, respectively. In 2006-2007, the exploitation of bony fishes resources started on 12 October 2006 and finished on 7 April 2007 in 2006-2007. 134 beach seines caught about 14120.0 tons of bony fishes by 48470 hauling. The total catch was estimated 23801.8 tones (Includes illegal fishery). Kutum, mullets and common carp comprised more than 98.5 percent of total catch. Length classes 39-40 and 38-39 cm predominated for kutum and common carp, respectively and length classes 27-28 and 29-30 cm predominated for golden grey mullet. Age groups 3 to 5 year comprised 80.2 and 71.8 percent for kutum and golden grey mullet, respectively and age groups 4 to 6 year comprised 71.8 percent for common carp. Age group 3, 4 and 5 year was dominant with 35.5, 32.1 and 27.1 percent for kutum, golden grey mullet and common carp respectively. K value and L∞ calculated 0.27 (/year) and 60.7 cm for Kutum, 0.2 (/year) and 58.4 cm for golden grey mullet and 0.19 (/year) and 66.7 cm for common carp, respectively. The total biomass and MSY estimated about 46654.9 and 14801.4 tones for Kutum, about 19549.0 and 5748.4 tones for golden grey mullet and about 10584.3 and 2186.0 tones for common carp, respectively. Exploitation rate (E) calculated 0.69, 0.53 and 0.52 for kutum, golden grey mullet and common carp, respectively.
    Keywords: Fisheries ; Iran ; Caspian Sea ; Bony fishes ; Kuttum ; Golden grey mullet ; Growth and mortality parameters ; Biomass ; MSY ; Common carp ; Coastal waters
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  • 24
    Publication Date: 2021-07-16
    Description: Experiments were conducted to evaluate the effects of different dietary levels of protein (%30, %35, %40, %45), fat (%8, %12, %16, %20) and total energy (3500,4000,4500,5000 kcal/kg) on growth of fingerling Roach (Rutilus rutilus caspicus knipowitschi ) in four treatments with triplicates. The crude protein of experiment, fish averaged 2.60 gr stoking density of 25 fish/tank in a during of 57 days, the crude fat of experiment, averaged 5.30 gr stoking density of 15 fish/tank in a during of 67 days and the gross energy of experiment, averaged 5.30 gr stoking density of 25 fish/tank in a during of 57 days which reared into 100L-fiber glass tanks. At the end of each experiments, growth average parameters, such as weight gain(%), specific growth rate, food conversion ratio, condition factor and survival (%) were not significantly different among all treatments (P〉0/05) but protein efficiency ratio showed in fish fed of %30 and %35 protein at levels significantly different with another treatments (P〈0/05). In all of experimentes, significant difference was found whole-body protein, lipid contents of fish fed the different dietary protein, lipid and energy levels indicated that significant difference among of treatments. As regards the economics of feeding and effective protein utilization, the dietary containing %30, %16 fat levels and total energy 5000 kcal/kg would be suitable to growth roach fingerling.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Protein ; Fat ; Energy ; Growgh ; Fish roach ; Rutilus rutilus caspicus knipowitschi ; Fingerlings ; Survival ; Lipid ; Feeding
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  • 25
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25569 | 18721 | 2018-10-06 17:07:25 | 25569 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The Caspian Sea is an important source of water in terms of having valuable resources of sturgeon and bony fish is taken into consideration roach(Rutilus rutilus caspicus (L.)) is one of the most important commercial species in the southern coast of the Caspian Sea in Iran , the catch of this species has declined substantially in the last 10 years, The management and exploitation need of comprehensive review of its biology .This study as part of a comprehensive study of feeding , spawning and growth of this species.In this study, using samples caught in beach seine along the southern coast of the Caspian Sea coastal ( Iranian waters ) took place. The fork length and total weight ranged between 12.5 to29.5(cm) and 29 to293( grm),10.5 to23(cm) and17.2 to21(grm) in Golestan and Gilan Province respectively. The b value of the length-weight relationship ranged 3.02 to 3.25 and 3.28 to 3.75 for female and male, in Golestan and Gilan Province respectively. The age composition of the catch was from 1 to 4 year in both Province, there was one spawning peak and Fecundity variations were high and ranged 7260 to 231965 eggs. Average growth in length was described with the Von Bertalanffy growth model: L (t) = 30.94(1-exp (0.42(t-0.18) and L (t) = 20.49(1-exp (0.53(t-1). The percent of empty stomach and prey dominant evaluated during different seasons by specific formula. Result showed that gastropod, Polychaete worms and molluscs were dominated and specific food items respectively. Shrimp, fish, insects, zooplankton and clams were scare of prey.
    Keywords: Biology ; Iran ; Caspian Sea ; Rutilus rutilus caspicus ; Spawning ; Feeding ; Growth ; Length
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  • 26
    Publication Date: 2021-07-16
    Description: Investigation on small pelagic fishes in the north of the Persian Gulf (Qeshm Island and Bandar Lengeh) coastal waters during October 2006 to September 2008, illustrated that 6 sardine and 2 anchovy species were locally existed. Sind sardinella (Sardinella sindensis) and Buccaneer anchovy (Encrasicholina punctifer) were dominated in this area. The peak of sexual maturity for Sind sardinella and Buccaneer anchovy occurred in spring and summer respectively. Reproduction period for Sind sardinella was from the middle of winter to late spring, and Buccaneer anchovy encompassed reproduction capacity all the year. The female: male ratio was calculated 1:0.7 and 1:0.9 for Sind sardinella, 1:1.27 and 1:1 for Buccaneer anchovy in Qeshm Island and Bandar lengeh sequentially. The mean of absolute fecundity were estimated 16234 and 16168 for Sind sardinella, 1277 and 1141 for Buccaneer anchovy in Qeshm Island and Bandar Lengeh respectively. L_M50 and L_C50 were calculated and the results showed both species mainly caught before length of maturity, and the mass populations have not sufficient opportunity to reproduction. Both species were planktonivore, theirs food prefect mainly consisted of copepods, naplius and bivalves. Vacuity index indicated both spices were semi-voracious appetite fishes. Based on calculated length-weight relationships in S. sindensis and E. punctifer, they have isometric growth. Growth parameters were estimated, the results illustrated that they were growth fast and short live species, which suggested S. sardinella and E. punctifer have 3 and 2 years maximum life span respectively. The recruitment pattern indicated double recruitment peaks per year in both species. Annually, four cohorts were distinguished in Sind sardinella and 3 cohorts in Buccaneer anchovy. Total mortality (Z), natural mortality (M) and fishing mortality (F) were estimated in both species, and the values of exploitation ratio (0.78 & 0.74) in Sind sardinella and (0.72 & 0.41) in Buccaneer anchovy were reasonable for current fishing effort due their short life span. Analysis of the results showed that CPUE in sardine and anchovy affected by reproduction and feeding processes. Results showed significant correlation between sardine CPUE and Sardine GaSI (P〈0.05, r= 0.499), similarly between anchovy CPUE and anchovy GSI (P〈0.05, r=-0.635). Further, significant correlation between sea surface temperature with sardine CPUE (P〈0.05, r=0.493), phytoplankton distribution (P〈0.01, r=0.560), anchovy GSI (P〈0.05, r=0.526), anchovy GaSI (P〈0.05, r=-0.500) and chlorophyll a (P〈0.01, r=0.780). Phytoplankton distribution with sardine GSI (P〈0.05, r=0.453), zooplankton distribution with anchovy GSI (P〈0.05, r=0.671), and chlorophyll with sardine GSI (P〈0.01, r=0.761) have significant correlation.
    Keywords: Fisheries ; Iran ; Persian Gulf ; Qeshm Island ; Bandar Lengeh ; Sardinella sindensis ; Encrasicholina punctifer ; Reproduction ; Feeding ; Growth and mortality ; Catch per unit effort ; Sea surface temperature ; Chlorophyll a ; Sexual maturity ; Plankton ; Naplius ; CPUE ; GSI ; Sardine ; Anchovy ; Coastal waters ; Surface ; Temperature ; Distribution
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  • 27
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25563 | 18721 | 2018-10-05 16:51:28 | 25563 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study, the characteristics of physico-chemical parameters of water done in coastal part of the southern Caspian Sea during 2010-2011 (four seasons) in 8 transects (Astara, Anzali, Sefidrour, Tonkabon, Noshahr, Babolsar, Amirabad and Torkman). For measurement of water quality parameters, 480 samples were collected in different water layers by using of APHA standards methods. According to modeling and statistical methods was used multivariate for evaluation of factor analysis (MFA) in general groups and discriminant analysis (MDA) for spatial and temporal with water quality parameters. The statistical procedure of MFA was used for data reduction and finally six factors selected with about 80.48 percent of total variances related to them. The statistical procedures of MDA were used for the role of spatial and temporal of water quality parameters in different water layer. The function one gave eight parameters (pH, salinity, EC, DO, NO^3-, water temperature, NH^4+ and N^-total) affording more than 80, 67, 80, 77 and 71 % correct assignations (return to the same transects) in spatial analysis. In all transects during four seasons, function one gave five parameters (water temperature, salinity, EC, NO^2- and NH^4+) to afford 100, 100, 100, 97 and 97 % correct assignations in temporal analysis. The results showed that, the average amounts in light penetration layers during four seasons were for EC(15.04±0.18ms/cm), pH(8.38±0.01unit), salinity (10.31±0.16g/l), DO(5.67±0.09ml/l) and water temperature (18.28 ±0.72°C). The average amount of nutrients for parameters such as organic and inorganic phosphorus, total phosphorus, NO^2-, NO^3-, NH^4+, N^-Total and SiO_2 were 0.50±0.02, 0.32±0.01, 0.82±0.02, 1.76±0.10, 0.10±0.01, 1.51±0.10, 46.82±1.80 and 8.60±0.29µM, respectively. The average amounts in dark layers during four seasons were for EC (15.46±0.38ms/cm), pH (8.34±0.03unit), salinity (10.70±0.35g/l), DO (4.55±0.13ml/l) and water temperature (9.62 ±0.16°C). The average amount of nutrients for parameters such as organic and inorganic phosphorus, total phosphorus, NO^2-, NO^3-, NH^4+, N^-Total and SiO_2 were 0.54 ±0.05, 0.33 ±0.02, 0.87±0.06, 1.92±0.21 , 0.08± 0.01, 1.55±0.25, 45.10±3.25 and 10.05±0.62 µM, respectively. The average amounts of light penetration in all transects during four seasons 4.49±0.38 meters. The maximum light penetrations during spring, summer, fall and winter seasons were 9, 8, 8.20 and 7 meters, respectively. The maximum water temperatures in light and dark layers were 32.36 and 11.50°C. Also, the maximum differences about thermocline temperature during summer and fall seasons were 19.6°C and 10°C, respectively in southern part of Caspian Sea. Data in spatial analysis were not significant (p〉0.05), it means if add or removed any transect did not change occurred. But in temporal analysis were significant (p〈0.01), and it means no way remove any seasons for a research work on Caspian Sea in southern part. In those transects with 100m depth. Water temperature is only highly effective parameter between other parameters. In function 1 between 3 functions in transects with 100m depth water temperature with 60% variance was the main role and effective between different seasons in depth part were very high.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Astara ; Anzali ; Sefidrour ; Tonkabon ; Noshahr ; Babolsar ; Physico-chemical parameters ; Nutrient maters ; Sea water ; APHA ; pH ; Salinity ; Temperature ; Nutrients
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  • 28
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25568 | 18721 | 2018-10-06 17:03:55 | 25568 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The beach seines catch and effort data from 1996 to 2011 categorized in four periods and used in this research. Geographical coordinates of beach seines determined by portable GPS and distribution maps of important and commercial species prepared by catch per unit effort (CPUE). The concentration of beach seines in the western coast of Golestan province, Kiashahr area and eastern part of Anzali was more than another part. The daily effort in Kiashahr and Anzali area was very high, thus the fishing stress in these areas was more than another part of Iranian coastal waters of the Caspian Sea. The number of species in catch composition increased from east to west and the beach seines of Guilan province had total of commercial species, but the number of commercial species rarely was five species in Golestan province and especially in eastern coast. Relative frequency and distribution of Kuttum in the east Babolsar area and then between Sepidroud and Anzali lagoon were more than another part. The ISI index of Kuttum was high in all of the area except east coast of Golestan province. The main distribution of Mullets determined in the western coast of Golestan province and the Amirabad fishing area and in these areas, the ISI index of Mullets was high. The main distribution area of common carp determined in east coast of Golestan and the ISI index of common carp was very low in other parts. Density and distribution of Kolmeh were high in eastern and western coasts of Iranian waters of the Caspian Sea and the density of this species was very low in Mazandaran province. The density of Zander was very high in surrounding of Sepidroud River and Anzali lagoon. The ISI index of commercial species in Guilan province was more than other provinces except Kuttum, mullets and common carp.
    Keywords: Biology ; Ecology ; Iran ; Caspian Sea ; Golestan province ; Guilan province ; Mazandaran province ; Babolsar ; Anzali ; Kiashahr ; Bony fishes ; CPUE ; ISI index ; Distribution map ; Species ; Fishing ; Kuttum ; Mullets ; Common carp ; Density
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  • 29
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25200 | 18721 | 2018-09-03 17:38:28 | 25200 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Anzali wetland at south west of Caspian located along of North West and southeast and with its unique feature were add on the Ramsar convention list in 1975. There were recorded a high rich of fauna and flora including 276 plant species, 23 reptiles and amphibians, 60 fishes, 26 mammals and 195 birds. Anzali wetland were encountered with numerous disorder with the eutrophication and the early dismissing. The first studies on Anzali wetland started by many international institutions in 1964. In this study the data of biotic and abiotic factors in Anzali wetland analyzed which belongs to different sites (15 to 38 stations) during 1991 to 2000 and were sampled seasonally or monthly in different years. The results showed the increasing trend of phytoplankton abundance from 7.9 to 66 million C./l. and the western part had the most abundant. The Cyanophyta had the most development than other 7 phylum observed in the area. In Anzali wetland identified 143 phytoplankton genus that 9 genus dominated in more than 80 % replicates and 7 genus had the abundance of more than 5 million cell/l. The Zooplankton abundant increased from 580 to 2200 ind./l. The Rotatoria had the most abundant followed by Copepoda. There was identified 104 zooplankton genus that 5 genus were dominated in more than 80 % replicates and 11 genus had the abundance of more than 30 ind./L. The chlorophyll a varied from 8.8 to 50.2 μg/l. and had a increasing trend after 1996, meanwhile it was measured higher than 25 μg/l in most of the stations. The results of benthic study showed that the Chironomidae and Tubificidae were observed more than other organisms which comprised 12.2 to 23.5% of total biomass of benthic organisms in studied period. The range of biomass was 1.1 to 7.8 g/m2. The ichthyologic survey showed presence of 60 species that 11 species were exotic. 28 species belong to freshwater, 17 species were anadromous and 8 species were resident in estuary. The highest and lowest fish catch were 640 and 288 ton in 1994 and 1999 respectively and it was about 500 ton at the end of period. The hydrochemical factors had the various fluctuation so that the nutrients parameters had a significant difference over studied period. Total nitrogen was varied from 0.78 to 1.41 mg/l and total phosphorus varied from 0.05 to 0.1 μg/l.. The oxygen dissolved amount was from 4.6 to 8.7. EC was between 3 to 5 ms/cm during September and October that indicated of Caspian water projection. According to geographic position of station, all biotic and abiotic data, were arranged. The data bank is used by Idrisi software to produce of necessary maps. Analysis of data showed that there must be a responsible department with a vast authority. Conservation and eutrophication prevention of Anzali wetland connect to special attention to catchment area and landuse management of the wetland. Also there is an urgent need for well-developed technologies and managements to reduce the organic pollution and its environmental impacts on the Anzali Lagoon.
    Keywords: Limnology ; Iran ; Caspian Sea ; Anzali wetland ; Ramsar ; Anzali Lagoon ; Limnological ; Survey ; GIS ; Fauna ; Flora ; Cyanophyta ; Phylum ; Phytoplankton ; Zooplankton ; Chlorophyll a ; Biomass ; Species ; Hydrochemical ; Nutrients ; Dissolved oxygen
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  • 30
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25201 | 18721 | 2018-09-05 16:03:37 | 25201 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Use and enrichment of live food resource in fish farms have been interested and highly demanded. Crustacean are one of the important groups. The Pontogammarus maeoticus dominated in southern Caspian Sea shore with a high abundance. This study was designed in order to adaptation and usage of amphipoda in fish culture ponds. The first part have been surveyed the laboratory experiments including of; to increasing and developing of P.maeoticus in 200 litter container, the effects of salinity on growth and survival of amphipods in many aquariums, the culture of common carp with amphipods and growth determination of them. Chemical composition analysis of P.maeoticus and carps fed by amphipods in compare to cultured carps from ordinary ponds. Due to concern about common healthy the heavy metal concentration has been measured in P. maeoticus, carp which were fed by amphipods and the cultured carps in earth ponds. In second phase; the adaptation of two amphipods species, P. maeoticus and Obesogammarus acuminatus was studied in fish ponds where some cages with sandy soft substrate had been provided for amphipoda replacement. Also a small surface of ponds surrounded by net and covered by Azola plant, a habitat suitable for to putting of O. acuminatus. Production of amphipoda had not the successfully results in large tanks. Aquariums with Caspian Sea water had the prosper results where the specimens were breeding and developing properly, even though in some aquarium with freshwater increased the amphipods number. The chemical composition had not significant difference between two kind of cultured carps while the organic component in amphipoda had a high quality. The better quality of cultured carp by amphipod diet have been confirmed by organoleptic test. The results of heavy metal measurement in amphipoda showed a high concentration which some of them were transmitted to cultured carps. Result of amphipoda replacement in cage was not satisfy and the specimens were died after some days. According to hydro-chemical parameters the oxygen poorness and high trophy levels were the affective factor to abolish of specimens in cages. It seems that there are many type of P.maeoticus that can be adapted in different salinities. The molecular differentiation should be investigated to choose the suitable type of this spices for utilization in freshwater fish ponds. In other hand it can be used in fish culture ponds that will be supplied by brackish water.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Adaptation ; Amphipoda ; Fish ; Culture ; Ponds ; Enrichment ; Pontogammarus maeoticus ; Amphipoda ; Survey ; P.maeoticus ; Common carp ; O. acuminatus ; Oxygen ; Specimens ; Brackish water
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  • 31
    Publication Date: 2021-07-16
    Description: In this study population genetic structure of Mnemiopsis leidyi were examined by 200 samples from the Caspian Sea. Samples were collected from Guilan province (Astara region), Mazandaran province (Amir Abad region), Golestan province (Gomishan region) and north part of the Caspian Sea. 50 samples were collected from each region. DNA was extracted by phenol - chlorophorm method and its concentration was 50 to 100 nanogram. PCR was performed using 18s rRNA gene. The PCR products of samples were digested by 12 restriction endonuclease enzymes. The digested products accompanied with standard marker (50 bp lader ). In order to measure fragment size samples were run on a 6% vertical polyacrylamide gel. The fragments were visualized by silver staining of the polyacrylamide gel. In this study, Dra I and Alu I enzymes showed different digestion pattern, as each of these enzymes had two genotypes. Other enzymes had similar digestion pattern. RAPD method used by 19 random primers. Ten of nineteen primers showed polymorphism patterns. Statistical analysis of data was performed by Popgene software. In this study, the maximum of genetic variation was in north of the Caspian Sea. Also, the maximum of genetic distance was between north area and Golestan coasts (0.089) and the minimum of genetic distance was between Mazandaran and Guilan coasts (0.001). The UOGMA dendogram showed two clusters. The samples of Mazandaran , Guilan and Golestan coasts placed in one cluster and samples of north area in other cluster The genetic diversity was significantly different between samples of north area and Golestan coasts (p≤0.05). As result, there is a significant genetic divergence between some of samples. Therefore, two genetic group of Mnemiopsis leidyi were identified in the Caspian Sea.
    Keywords: Biology ; Iran ; Caspian Sea ; Guilan province ; Mazandaran province ; Golestan province ; RFLP ; RAPD ; Mnemiopsis leidyi ; Genetic variation ; Population ; Molecular Test ; Samples ; Enzymes ; Genotype
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  • 32
    Publication Date: 2021-07-16
    Description: After the arrival of the invasive spexies (Mnemiopsis leidyi) in the ecosystem of Caspian Sea in 1999, It had notable impacts. In this study, we,re assessing impacts of quantitative M. leidyi as a biopllutant on zooplankton community in the southern part of the Caspian Sea during 2001-2010 period. It becomes clear that Abundance and Distribution Range (ADR) of M.leidyi during 2001-2009 was in class E(occurrence in high numbers in all localities) and in year 2010 was in class D(occurrence in moderate numbers in all localities). from 2001- 2009, ADR in Summer season was in class E, but in summer 2010, relatinve biomass of M.leidyi has decreased and ADR was evaluated as D. During the fall seasons of 2001 -2010, only in fall of 2003 and 2010, the ADR was C(occurrence in low numbers in all localities) and D respectively and in other years, the ADR was in class E. In winter season relative biomass of M.leidyi decreased and only in winter of 2001 relative biomass was high and the highest ADR (class E). In winter 2003 and 2004, ADR was in class D. The biomass of M.leidyi was found to be zero in winter 2008. From winter of 2009-2010,ADR, were assessed B and A respectively. In the spring, ADR was evaluated A and D. Considering the obtained results, the yesr 2001, which the relative niomass was more than 90 percent, could be considered as the expantion phase and the following years (2002-2010) with regard to decrease of relative biomass as the adjustment phase. With regards to the loss of the keystone species (Eurytemora sP.) and some other native species, our evalution showed that the impact of M.leidyi on structure of zooplankton community was assessed as massive (C4).During summer and fall seasons that biomass of M.leidyi was higher than 50%, only A.tonsa exist, but during spring and winter seasons that biomass of M.leidyi was decreased and more species have a chance to exist.
    Keywords: Ecology ; Iran ; Caspian Sea ; S Caspian Sea ; Mnemiopsid leidyi ; Zooplankton Community ; Native species ; Bioinvasions
    Repository Name: AquaDocs
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25618 | 18721 | 2018-10-07 16:34:53 | 25618 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Chitin and chitosan are 2 very important products of biopolymer that enjoy high consumption in industry, but their production sources are very limited. In this study, Artemia urmiana cyst shells were obtained from previously collected and stored ones in Iranian Artemia Research Center. 20 kg of Artemia urmiana cyst shells were sampled, cleaned, separated, dried and transferred to Iranian Artemia Research Center Laboratory to extract their Chitin and chitosan. Their chitin and chitosan initially were extracted using optimized common chemical methods. Their properties were compared to 2 other types of Chitin and chitosan obtained from crab and shrimp manufactured by Vietnam and China, respectively. To determine their quality, elemental analysis device, infrared spectrophotometry, x –ray radiography, determination of viscosity , molecular weight, crystallinity percent, color, de stylization measure, empirical and molecular formulas were made. The results showed that the percentage of chitin obtained from Artemia cyst Shells in Chemical method was 28 ± 3 % by weight and efficiency into chitosan (grade steel relief) in this method was 50± 5%. To optimize the extraction procedure and the removal of proteins of chitin by biological practices that were done by sodium hydroxide in the chemical method, it was replaced by the bacterium Bacillus subtilis. And in the bio- phase of chitosan de steelation fungus Aspergillus niger enzyme was replaced instead of sodium hydroxide at high temperatures. The results showed that chitin and chitosan can be extracted from Artemia cyst shell using biological method and their characteristics included as in chitin 49.6% C, 8.2 % N, 7.5 % H, and 34.5 %O. Also the same levels for chitosan were 44.4 %, 8.9, 7.2 and 39.5 %, respectively. Their other quality characteristics were included chitin average molecular weight 4.9×10^6 Dalton, crystallinity percentage of 36.4, viscosity at 20°C 31 centipoise and its color was gray to brown. In the biologic method, the average molecular weight of chitosan, crystallinity percentage, viscosity at 20°C, were 5.1×105 Dalton, 94.5, and 18 centipoises, respectively. Also, its color was pale brown. Chemical structure of extracted chitin and chitosan from the shell of Artemia urmiana cysts were C_7H_12NO_4 and C_6H_11 NO_4c, respectively. The comparison of chitin and chitosan obtained from each chemical and biological method revealed that replacing biological methods instead of chemical methods is possible in achieving these products at suitable condition and better quality. This can eliminate the use of chemicals damaging the environment such as sodium hydroxide and decrease environmental pollution.
    Keywords: Biology ; Chemistry ; Iran ; Artemia urmiana cyst shell ; Chitin ; Chitosan ; Chemical ; Biological methods ; Crab shell ; Shrimp shell ; Biotechnology
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25612 | 18721 | 2018-10-07 16:15:05 | 25612 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Kutum fish (Rutilus kutum) is an anadromous cyprinid species that spawns in adjusted rivers to the Caspian Sea. Many million fingerlings were artificially produced and annually released in these rivers specifically in Sefidrud River by Iranian Fisheries Organization. In this study were estimated the staying period of Kutum fingerlings in Sefidrud River and its diet and growth. A small piece of the caudal fin was cut as a tagging method. About 50000 and 5200 individuals were marked during July and September 2013, respectively. The marked fish had an average total length 53.2±5.6 mm and body weight 1.11±0.26 g and were released in river around two kilometers of the estuary. The sampling of fishes had been earlier started from Feb. 2012 and continued until Feb. 2013 at five stations. Four stations were along river from three Kilometer of estuary to estuary and one station was in right side of estuary in the sea coast. The results showed that marked samples after releasing time were distributed in all studied area from 3 kilometers of estuary to estuary but they migrate mainly toward Caspian Sea in a short time. However the marked samples were observed in estuary and the sea coast after 6 and 24 hours of their releasing time, respectively, Kutum fingerlings remained in Sefidrud river ecosystem in a longer time, at least until end of our sampling period i.e. 105 days. The coefficient of vacuity index of the natural feeding study was 0.0, 49.0 and 30.6 % for phytoplankton, zooplankton and benthos, respectively. The average of GastroSomatic index, intensity of fullness and condition factor were calculated 6.30±3.25, 174.6±153.2 and 0.92±0.09, respectively. A temporal and spatial variation on main food items were observed in gut content of fish fingerlings from different locations and seasons. Gut contents were generally included 59 genera of phytoplankton, 15 groups of zooplankton and 10 groups of benthos. Nitzschia, Navicula and Synedra were dominated among phytoplankton genera with 45.83, 18.02 and 15.99 %, respectively. Among zooplankton groups; the Rotaria, Moeina and Difflugia were dominated with 22.30, 20.86 and 15.83%, respectively. Chironomidae and Gammaridae were the main food items among fed benthic animals with 83.67 and 11.15%, respectively. Furthermore 48 phytoplankton genera, 32 zooplankton genera and 6 benthic families were identified in ecosystem of Sefidrud river. Bacillariophyta phylum (with 23 genus), Rotatoria (with17 genus) and Chironomidae family were dominated in each mentioned groups, respectively. It is concluded that the Kutum fingerlings stay in Sefidrud River for a long time, although most of them migrate to Caspian Sea during first month after releasing. However dietary indices were normal in studied samples, the condition factor index seems to be low.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Rutilus kutum ; Fingerlings ; Kutum ; Fish ; Rotaria ; Moeina ; Difflugia ; Phytoplankton
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25617 | 18721 | 2018-10-07 16:24:05 | 25617 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The project is funded by the CASPECO Project which is the Third Phase of the GEF supported project in the Caspian Sea region titled “The Caspian Sea: Restoring Depleted Fisheries and Consolidation of a Permanent Regional Environmental Governance Framework”, and implemented by the Iranian Fisheries Research Organization (IFRO) in partnership with four Coastal Research Centers affiliated to IFRO, Coldwater Fishes Research Centre (CFRC).The project's objective is to improve the conservation and restoration of Caspian Salmon (Salmo trutta caspius) stocks. In accordance with IUCN criteria Caspian Salmon is placed in the red list of endangered fish. In the recent decades the destruction of habitats for juveniles, reduction of genetic diversity and fish immune system were main causes of the Caspian salmon decline. Numbers of public meetings were held with participation of fishing communities’ representatives, experts, relevant local authorities and experts of the IFRO and Iranian Fisheries Organization (IFO), Iran Environment Protection Organization (IEPO), Water Resource Management (Ministry of Energy) and University senior professors, in order to create interaction and understanding of the project objectives. Educational awareness and extension program were conducted with the involvement of fisheries communities, private sector and NGO associations. The results of the project suggests that cross-sectoral coordination for sustainable fisheries can be achieved through building partnerships and enhancing communication between the environment, private, fisheries sectors and academia. Cross-sectoral cooperation through this project improved public awareness on growing risks facing Caspian Salmon from different sources including local sources (illegal fishing and over-fishing, urban wastewater, environment destruction, industrial pollutants, pollution of air and water resources), new emerging and invasive organisms, climate change, implications of the Caspian salmon population decline on economic and social affairs of the people who depend on them for their livelihood and food security and their role in fish stock protection. The project, through its capacity building, extension and educational programs and efforts to link science to management, could translate scientific findings into an action agenda for managers and policy makers that can bring an improvement in the future of the Caspian Sea salmon stock.
    Keywords: Management ; Iran ; Caspian Sea ; Conservation ; Salmo trutta caspius ; Caspian salmon ; Fisheries ; IFRO ; Juvenile ; Genetic diversity ; Immune system ; Illegal fishing ; Pollution
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25622 | 18721 | 2018-10-08 05:50:41 | 25622 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The overfishing and changing environmental following the introduction of the comb jelly Mnemiopsis leidyi in the Caspian Sea, the population structure has a strong influence on kilka fishes in the Caspian Sea and therefore to in this investigation which had been done in commercial catch regions (where discharged theirs catch) in three ports Babolsar, Amirabad (in mazandaran) and Anzali (in Guilan), stimated catch and catch per unit effort, the age structur of catch, length- weight relationship, von Bertalanffy growth parameters, condition factor, sex ratios, maturity stags, spawning and feeding. The result shown that catch of kilka in Iranian coastal in 2012 decreased from 24080 ton to 22696 ton in 2013. But CPUE increased was 2.7 ton (Vessel ×Night) and there was no significant changes. Common kilka was the predominant all months of the year, 98.1 and 98.9 percent in 2012 and 2013 respectively. The mean length was 104.4±10.5 and 101.6±11.2 at the same time and range length 92/ 5 – 117/5 mm were the dominant population(about 75%). Relative abundance of fish less than 77.5 mm and more than 127.5 mm is very low. Age abundance during this period have not been substantially changed and always fishes with 3 and 4 year olds had the highest frequency73.6 and 73.5 percent respectively. The parameters of the Von Bertalanffy growth curve were (8.141) 048.1 for common kilka. The instantaneous coefficient of natural mortality (M) was 0.506 yr-1. The instantaneous coefficient of fihing mortality and total mortality were 0.694 yr-1 and 1.2 yr^-1 , respectively. The exploitation rate of common kilka varied during 2012-2013 between 0.41-0.50. The mean condition factor 1.41±0.14 (n=5801) and 1.38±0.16 (n=6754) at the same time respectively. According to statistical analysis Anova oneway were a significant difference between condition factor at the same time . Main prey common kilka was Acartia tonsa accounting for over 80%. It seems Common kilka due to a decrease in the frequency of other zooplankton species depend more than ever on Acartia tonsa and this species is probably a concentration of prey for Common kilka.
    Keywords: Biology ; Fisheries ; Iran ; Caspian Sea ; Mazandaran Province ; Guilan Province ; Anzali ; Babolsar ; Kilka fishes ; Age ; Growth parameters ; Condition factor ; Exploitation rate ; Feeding ; Reproduction ; Overfishing ; Comb jelly ; Mnemiopsis leidyi ; Population ; Sex ratios ; Maturity ; Spawning ; Mortality ; ANOVA ; Acartia tonsa
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25229 | 18721 | 2018-09-06 06:14:35 | 25229 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Study and recognition of the Quanat water of Rafsanjan, Sirjan and Ravar cities has been beginning since the November of 2004. Among 228 Quanat chains were studied in these cities, 165 quanat chains had a discharge more than 10 liter per second (L/Se) which were sampled. In this research oxygen, pH, discharge, water temperature and air temperature were measured in two times and in the local place. Height from the sea level, longitude and latitude were measured (by GPS Machine) in one time and in the local place and other physical and chemical factors including No_2, No_3, Co_2, total hardness, NH_3 and So_4 were measured one time in the laboratory. In this manner, the condition of connection ways of considered Quanat with city was studied. With examination on the Quanat water in above said cities, all of Quant was within desired extents for aquaculture activities. Moreover in some of them, water temperature was higher than 20 degree centigrade that with some changes and in specific months can be used in aquaculture.
    Keywords: Biology ; Iran ; Rafsanjan ; Sirjan ; Ravar ; Fish ; Aquaculture ; Quant ; Water ; Oxygen ; pH ; Temperature ; No2 ; No3 ; Co2 ; NH3
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25627 | 18721 | 2018-10-07 16:48:29 | 25627 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study have been conducted entitle biological and statistical sturgeon in the southern Caspian Sea (Mazandaran waters)” from 2009 to 2013. Sampling was manthly. Overal, 1859 specimens of different species of sturgeon was caught including Acipenserpersicus (69%), A. stellatus (19%), Husohuso (6%), A. nudiventris (4%) and A. guldenstaedtii (2%). Totally, the meat and caviar of five species were found 53263.6 kg and 5633 kg respectively and caviar to meat ratio was 10.6%. The results show that ration of meat and caviar whole species reduced from 2009 to 2012 in which the meat and caviar of A. persicus was declined from 12146 to 4465 kg in meat and caviar from 1358 to 487 kg were extremely reduced respectively. The CPUE (catch per unit effort) of sturgeon has fluctuated from 2009 to 2012. Totally, the CPUE of A. persicus was decreased from 0.150 (2009) to 0.130 (2012) kg boat per day, A. stellatue from 0.009 (2009) to 0.021 (2012) kg boat/day, A. guldenstaedtii from 0.002 (2009) to 0.003 (2012) kg boat/day, A. nudiventris from 0.027 (2009) to 0.001 (2012) kg boat/day and H. huso from 0.123 (2009) to 0.018 (2012) kg boat /day. 1061 specimens of fish has been caviar including A. persicus with 69%, A. stellatus (18.1%), H. huso (5.5%), A. guldenstaedtii (2.7%). The frequency of caviar categorize was included 1 , 2, 3 and massive with 49.6%, 38%), 10.6% and 1.9%, respectively. The highest of meat and caviar belong to A. persicus with 31414 kg and 3515.7 kg, respectively were found and the lowest was 841kg and 105.9 kg pertain to A. guldenstaedtii. The results show that 65.9% of sturgeon was caught by legal catch (landing) and 34.1% by beach seine remained. 391 individuals was age determined that the minimum and maximum age was 9 and 41 year comprised to H.huso and A. stellatus, respectively. A. persicus and A. stellatus were majority caught compare to other species. A. persicus has the highest frequency in 14-15 year age groups with 50% and 57% in 2009 and 2011, respectively and 15-16 year age group with 41% and 56% in 2010 and 2012, respectively. The results show that mean of fork length, body weight, caviar weight of A. persicus was declined because the FL and weight average (±SD) obtained from 152.4±16.7cm and 25.8±9.2 kg in 2009 to 146.5± 18.7cm and 22.1±9.2kg in 2012, respectively. In contrast, the average (±SD) FL and weight of H. huso was increased from 218.8±41.1cm and 137.3±96.6kg in 2009 to 231.3±45.2cm in FL and 146.2±78.5kg body weight in 2012, respectively. Consequently, the results show that stocks of sturgeon in the Caspian Sea has a trend declined because of some species in IUCN categorize list was Endangered in 2006 but at this moment lie in Critically Endangered list of IUCN categorize were down which could be a serious alarm for the valuable sturgeon fish in the Caspian Sea. Therefore, recommendation that trade catch of sturgeon in the Caspian Sea must be forbidden and continue legal catch for spawner fish that needede to artificial propagation only.
    Keywords: Biology ; Iran ; Caspian Sea ; Biology ; Sturgeons ; A. persicus ; A. stellatus ; A. guldenstaedtii ; A. nudiventris ; H. huso ; CPUE
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25245 | 18721 | 2018-09-07 07:22:16 | 25245 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Annually, many data of hydrology and hydrobiology of the southern Caspian Sea at Iran's coasts were collected by Caspian Sea Research Institute of Ecology. The data which had been collected by researchers submitted annually in several reports but has not been processed all at once. Fluctuations of various parameters were surveyed and the relationships between them were analyzed. The main goal of this research is to analysis the data were obtained from 1994 to 2006 and show changes the concentration of various parameters in the Caspian Sea. Physicochemical data indicated that the average trend of transparency and salinity was decreased from 1994 to 2006 annually, where the salinity value was decreased from 12.37 ppt (part per thousand) to 11.5 ppt. The average of dissolved oxygen was increased from 6.4 to 8.6mg/l. pH has slightly fluctuated from 8.15 to 8.31. However, with increasing depth from surface to bottom, the average of dissolved oxygen was sharply decreased (DO concentration in surface and 800 m was 7.18 and 1.45 mg/l, respectively). The trend of salinity and pH fluctuation was very slow with depth but the salinity has an increasing with incrementing of depth but pH has a decreased trend. The average of organic and inorganic phosphorus and TP was nearly increased. The average of Nitrate and Silica sharply increased comparing to depth but the average of Nitrite and organic Nitrogen decreased. The results showed that the temperature fluctuation in different water layers was low in winter but the dramatic decreasing of temperature was occurred at 10-20 m (in spring), 20-50 m (in winter) and 50-100 m (in autumn). With increasing of depth (Slope of the Sea), transparency and salinity values have an increasing while DO and pH showed decreased. As a whole, 335 species of phytoplankton were identified, there are 70 species in advance as these species were not seen in this period but 96 of novel's species have been recorded. The lowest and the most of phytoplankton biomass was recorded in 2006 and 2001 while the biomass was 59 and 1034 mg/m^3, respectively. Albet, the lowest biomass was recorded at the depth of 100 m (24.1 mg/m^3) but the maximum was observed in surface layer (1344.1 mg/m^3). The average of phytoplankton biomass was increased after arrival of the ctenophore. There was a significant difference between the average of biomass in different seasons before and after of the ctenophore invasion (P〈0.001). Bacillariophyta and Pyrrophyta have the most cell abundance with a total of biomass of 52.7 and 37, respectively. From 1994 to 2006, species diversity (Shanon-Niner), evenness and richness were estimated between 3.02-1.29, 0.28-0.61 and 4.32-7.60, respectively. A total of 65 zooplankton species were identified with a frequent species in the Caspian Sea before the ctenophore invasion, while the species diversity decreased after the invasion. The high and the lowest of species diversity was recorded in 1994-1995 and 2006 and in 1999 the biomass of the zooplankton were observed between 12.6 mg/l and 363.8 mg/l, respectively. After arrival of ctenophore, the averages of zooplankton biomass at of the different depths were sharply decreased and were less than the ctenophore invasion. The result showed that there was a significant difference among the average of zooplankton biomass in two periods, seasons and west, middle and east regions as follows: P〈0.001, P〈0.008 and P〈0.01. The maximum abundance and biomass of zooplankton belonged to copepoda with %51.5 and %63, respectively. Rotatoria falls in the second class from 1994 to 2006 (during 1994-2006), species diversity, evenness and richness were varied between 0.19-1.6, 0.08-0.52 and 0.37-2.29, respectively. The species diversity of macrobenthic organisms at the same period s followd by zooplankton changes which sharply decreased. The average of benthic biomass reduced from 13.7 g/m^2 in 1994-1995 to 1.8 g/m^2 in 2004 but sharply increased in 2005 with a maximum value of 46.7 g/m^2. With increasing of depth, the average of benthic biomass was sharply decreased. There was a significant difference (P〈0.001) in macrobethic organisms abundance before and after the ctenophore invasion, but it did not show a significant difference between seasons and different regions (as follows: P〉0.137, P〉0.782). Before the presence of ctenophore, the worms had been constituted a dominate group with an approximately %45 of total abundance and also %20 of their biomass. After this period, their frequency was sharply increased (more than %85) but the maximum value pertains to Cardidae (more than %90). The species diversity, evenness and richness were varied between .69-2.51, 0.23-0.63 and .011-3.79, respectively. AMBI software, Shanon-Viner parameter was moderate in all seasons from 1994 to 1996 but this parameter was reduced in a bad limit (boundary) in the most seasons from 2002 to 2005. The main parameter of M-AMBI consists of several parameters and also approximately showed similar changes such as Shanon -viner parameter. On basis of this parameter, the ecological quality condition of Caspian Sea was superior limit in all seasons from 1994 to 1996 but after the following years were decreased from good to moderate limits (After the presence of ctenophore). Relationship between abiotic variants and phytoplankton indicated that there was a direct relationship between transparency and salinity while there was a powerful and reversal significant relationship between transparency water temperature, transparency in organic Nitrate, phytoplankton number and transparency phytoplankton biomass. The comparison between different variants average in two periods (before and after the ctenophore invasion) indicated that the average of Kilka catch was decreased, the relative frequency of clupeonella engrauliformis and clupenoella grimmi was sharply reduced but the relative frequency of Clupeonella cultriventris was sharply increased, the species diversity, evenness and richness and the number of zooplankton species were sharply decreased, the average of biomass and transparency was sharply reduced, the average of dissolved oxygen and liza saliens or liza auratus catch were increased. These difference were often significant (P〈0.05). Therefore, with the ctenophore invasion into the Caspian Sea, the primary production was increased, the biomass value of zooplankton and specially Kilka which fed on zooplankton were sharply decreased while the fish such as Rutilus frisii kutum and mullet (Liza salins or Liza aurratus) which fed on benthic were increased.
    Keywords: Ecology ; Iran ; Caspian Sea ; Transparency ; Salinity ; Dissolved oxygen ; pH ; Nitrate ; Nitrite ; Species ; Phytoplankton ; Biomass ; Bacillariophyta ; Pyrrophyta ; Diversity ; Zooplankton ; Rutilus frisi kutum ; Liza salins ; Liza aurratus
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25253 | 18721 | 2018-09-07 06:53:45 | 25253 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Acipenser persicus, one of the native Species living in the southern part of the Caspian Sea, has high Percentage of larval mortality. Therefor to decrease the rate of mortality of larvae survival and to increase to regenerate properly during the active feeding process of fish larvae, while under artificial reproduction they were fed withe fresh water rotifer. Four types of feeding treatments were applied as follow: Treatment 1: Similar to normal process, first withe Artemia cyst then withe Daphnia Treatment 2: Mixture of Artemia, Rotifer and Daphnia; Treatment 3 freshwater Rotifera ;Treatment 4: fresh water Rotifer enriched with Vitamin C (Ascorbic acid- 6- Palmytat).Three replicates were used for each treatment. In the process of each observation 45 larvae in containers withe 100 liter capacity filled with 30 liters of water, after 8 days of external feeding process, were inspected closely, during which , they were under biometric measurement once every two days. Larvae were fed Four times a day at a rate of 25% of their body weight. The average temperature estimation was 22.5±0.5 centigrade, pH .of water being 85±0.1 while Oxygen proved to be 9.58±0.2 mg/l. At the end of the observation, the profile fatty acids of the remains were studied. The growth factors, the mouth size of the larvae and the expansion of the mouth size compared to the total length of the larvae during the whole observation were inspected. Credibility of data designated was examined by Shapiro Wilks test. Specific growth rate (SGR), weight growth (WG) and feed conversion ratio (FCR) were contemplated by one-way analysis test, and significant difference Tukey's test. The most notable diversity was evident between treatment 2, whose indicative factors were in the following order: 4.65± 0.06, 45.18±0.66, 4/48 ± 0/07 and treatment 4 whose indicative factors showed the following results: 10.47±0.04, 124.42±0.62, 1.51±0/008.For the determination of fatness rate and weight gains, Kruskal Valis and Man vit ni test were used .In this part of study , the highest diversity fatness appeared to be between treatment 4 ,reflecting 0.79±0.07 and treatment 2 showing the result 0.62±0.05.The highest gain was related to treatment 4, whit the rate of 99.33±0/68 mgr and the lowest gain occurred in treatment 2 white the rate 62.36 ± 0.65 mgr. This study also revealed that, the survival ratio connected with treatments 3 and 4, compared treatments 1 and 2, were significantly different. The profile of fatty acids also showed much higher percentage of PUFA and HUFA in treatments 3 and 4, than those in other treatments. Furthermore Brachionus calyciflorus proved to be a proper food supply for larvae. Therefore by building suitable pools next to the work side, other kinds of species fresh water rotifer could also be used as food improvements for feeding larvae.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Brachionus calyciflorus ; Acipenser persicus ; Fatty acid ; Larvae culture ; Vitamin C ; Larval survival ; Species ; Mortality ; Feeding ; Artemia ; Cyst ; Ascorbic acid ; Oxygen ; Growth ; SGR ; FCR ; Fresh water
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25642 | 18721 | 2018-10-14 02:40:17 | 25642 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Padina boergesenii is one of the most abundant brown algae distributed in the north of Persian Gulf and Oman Sea. In this study after sampling and preparation of Padina boergesenii by Chroform-Etanol (3-1) solvent and by Methanol has been extract. Separation and purification of the compounds was carried out using thin layer, general and inverse column chromatography, Cephadex and high-performance liquid chromatography (HPLC). Structural elucidation of the constituents was based on the data obtained from H-NMR, 13C-NMR, HSQC, HMBC, DEPT and Cephadex LH-20. The steroids compounds separated from above alga were identified as 22dehydrocholesterol (1), cholesterol (2), fucosterol (3), β-sitosterol (4), stigmasterol (5), ostreasterol (6) and two epimer of hyroxyestrol(7), based on their spectral data and from comparison with those previously reported in the literature.
    Keywords: Biology ; Chemistry ; Iran ; Oman Sea ; Persian Gulf ; Brown Algae ; Padina boergesenii ; Steroids compounds ; Extraction ; Purification ; Identification ; Amount verification ; Sargassum glaucescens ; Algae ; Abundant ; Sampling ; Chromatography ; Cephadex ; Dehydrocholesterol ; Cholesterol ; Fucosterol ; β-sitosterol ; Stigmasterol ; Ostreasterol
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25792 | 18721 | 2018-10-13 08:54:26 | 25792 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Cheshmehkileh River and adjacent mountainous streams, play a strategic role as a historical axis for anthropogenic civilization, human welfare also habitat and migration pathway of commercial – biologic valuable fishes e.g. Caspian trout, Caspian kuttum, members of Cyprinidae family in south Caspian Sea drainage. Treats such as overfishing of Caspian trout and Red spotted trout stocks in mountainous headwaters, barriers construction and manipulations those are out of river carrying capacity developed by human activities, affected normal function of river as well. Sand mining big factories establishment next to the river, legal and illegal trade of river sediments, direct entry of Tonekabon landfill leakage into the river, development of Rainbow trout farms since 3 decades and huge effluents into the river containing dead fish and types of solids, escapement of cultured Rainbow trouts into the river, … are major minimum factors which needs basic information for integrating inclusively drainage management system. Cheshmehkileh River contains Headwaters of Dohezar (Daryasar & Nusha), Sehezar and Valamroud rivers during 13 monthly sampling phases between September 2009 and October 2010 based on macrozoobenthoses investigations by EPT, EPT/C EPA protocols, measurements of nominated physic-chemical and microbiologic parameters. Probability of Rainbow trouts escapement and invasion, existence, nutrition in Cheshmehkileh environment indeed investigated. Data analysis explained significant differences (P〈0.05) between groups of measured parameters in different sampling stations. Dendogram of clustered analysis based on consolidation of major biologic/ physic-chemical and microbiologic parameters, separated stations No. 1, 3, 2, 4 in one group and remained classified in different groups. Station 8 and 9 similarly separated which expressed general similarities according to Sehezar river environment which were differs in comparison with other stations. Station 11 separated according to its natural quality of water and environment. Similarities between station 10 to Sehezar river stations 8 and 9 expressed general influence of Sehezar River more than Dohezar River in Cheshmehkileh condition especially in station No. 10. High scores of EPT and EPT/C indices in upstream stations 1, 3 and 8 also low score of indices in stations 7, 13 and 6 expressed levels of environment quality between these groups of stations. Maximum average biomass of macroinvertebrates belongs to Trichoptera order in Cheshmehkileh River. Significant decrease of biomass in stations 11, 12 and 13 in comparison with other stations stated environment degradation in mentioned stations relevant to excessive sand mining as well. Pollution resistant groups of invertebrates significantly increased in downstreams against upstream stations. Also disappearing of Plecoptera order in station No. 7, 9, 10 and 13 stated low quality of environment in comparison with upstream stations. Confirmation of effects quality and quantity for point and non-point sources of imported pollutants require specific management considerations in order to present exploitations, pollutants control and emergencies for river monitoring in forthcoming years.
    Keywords: Aquaculture ; Management ; Iran ; Tonekabon ; Cheshmehkileh ; River ; Pollution ; Aquaculture ; EPTC ; Assessment ; Macroinvertebrates ; Chemistry ; Microbiology
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25818 | 18721 | 2018-10-13 10:23:49 | 25818 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The ecological study of the Persian Gulf Martyres Lake carried out at the 5 stations between 2013 and 2014. This study identified 35 phytoplankton taxa. The diatoms taxa was dominated and their abundance recorded highest (2000 000 cell. L^-1) in the lake. The annual phytoplankton abundance was measured as 2500 000 cell. L^-1 during the study. The total nitrogen and water temperature were the significant a biotic parameters to increase cyanophytes abundance. Furthermore, 37 zooplankton taxa were identified. The Rotatoria abundance was dominated zooplankton. The annual zooplankton abundance was measured as 72 ind.l-1. Based on the CCA, there was no correlation between Rotatoria abundance and a biotic parameters. The study benthos showed Ephemeroptera and Diptera abundance were dominated; artificial bottom and lack of sediment and organic matters were the main reasons in decreasing of the benthos density. The Chitgar lake is the poorest lake in Iran due to low density of plankton and benthos organisms. The estimation of fish production was 123 kg/ha and for the lake was determaind 16 tonne. The finding display, invasive species was the main fish Chitgar lake that would be negative effect and increase eutriphication trend in the lake. Phosphorus parameter was limited parameters and trophy level recorded low due to high N/P ration in the lake. In overall the Chitgar lake situation is in Oligotrophic category with the low trophy level.
    Keywords: Ecology ; Iran ; Persian Gulf ; Chitgar Lake ; Phytoplankton ; Zooplankton ; Benthose ; Trophy ; Abundance ; Nitrogen ; Temperature
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25821 | 18721 | 2018-10-13 10:32:05 | 25821 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: A mass-balance Ecopath model of coastal areas in the Caspian Sea has been constructed for a quantitative description of the trophic structure of the ecosystem. The model is used to estimate the important biological parameters and relationships among the different ecologically important groups. Twenty five species were used in the present analysis. The model is based on the data collected of Kilka fisheries, beach seining (Bony fish fisheries), and set Gillnet (Sturgeon Fisheries) and also dietary information. Total landings along the Iranian portion of southern Caspian coast reached 39647 t, including sturgeon (41 t), kilka (22873 t) and bony fishes (16733 t) in 2014. Two species of kilka (Common kilka and Anchovy) are important commercially in the Caspian Sea, together accounting in the past decade for 〉 60 % of the total catch, as well as being a crucial part of the food chain. The mean average trophic level was estimated at 3.17 by Ecopath software. In this research, the mean level were studied between eight species varied from 2.56 to 4.04, Sander lucioperca occupy the highest and the as vimba vimba was the lowest level. The ranges of total mortality varied from 0.5 to 2.56 per years. The food consumption rate was estimated about 101.56 per year Mixd trophic level index (MTI) showed small pelagic fishes as prey, have crucial role in feeding of pelagic predators population such as invasion of the ctenophore Mnemiopsis leidyi, bream fish and benthic fish such as sturgeon. In general, niche overlap was greater in more species such as Alburnus chalcoides, Rutilus rutilus, Clupeonella cultriventris, Rutilus kutum and M. leidyi who consumed large amounts of plankton. In conclusion small pelagic fish exert a major control on the trophic dynamics of the Caspian Sea ecosystems and constitute midtrophic-level populations.
    Keywords: Ecology ; Fisheries ; Iran ; Caspian Sea ; Trophic level ; Food consumption ; Total mortality ; Interactions ; Marine ; Food ; Food webs ; Pelagic ; Fish
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25822 | 18721 | 2018-10-13 10:12:48 | 25822 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The Anzali wetland (37.28N – 49.25E) with less than 100 km2 surface area is located in the southwestern shores of the Caspian Sea in the Guilan province. The benthic macro-invertebrates were studied as a part of a main project titled study of aquatic communities variation in the Anzali wetland. The sampling was performed at the 10 stations with using a Van-Veen grab during 2014-2015. The results showed, 14 macro-invertebrates taxa were identified as Chironomidae (Insecta, Diptera) and Tubificidae (Annelida, Oligochaeta), they were dominated in the wetland. The maximum and minimum densities of macro-invertebrates were observed with 609±1119 and 93.32±27.92 n/m^2 respectively at the stations 5 and 1. The silt-clay constitute more than 85 percentage of the wetland sediments with maximum of 93.2±27.92 percentage at the 2 station and minimum 72.21±27.92 at the 5 station. Kruskal Wallis non parametric test (sig. ≤0.05), revealed significant differences for all studied factors at stations while of %tom and grain size had no significant variation. The CCA analysis revealed no accordance of total organic matter (TOM ) and silt- clay percentage vectors with Tubificidae and total benthic frequencies, while Chironomidae frequency is in accordance. Intensifying eutrification process in the Anzali wetland has affected its biota as the benthic fauna are almost limited in to most tolerant taxa which reflect the day after day increasing pollution the Anzali wetland.
    Keywords: Biology ; Ecology ; Iran ; Caspian Sea ; Guilan Province ; Anzali wetland ; Macroinvertebrates ; Eutrification ; Aquatic ; Tubificidae ; Chironomidae ; Insecta ; Diptera ; Annelida ; Oligochaeta ; Benthic fauna
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25828 | 18721 | 2018-10-13 10:19:08 | 25828 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Different studies on Litopenaeus vannamei (Boone, 1931) has performed in Iran, but less noted in water quality conditions. Water quality, in addition to creating optimal environment for the growth of vannamei shrimp, as well as cause the live food production and improve economic performance are produced. This study was conducted to evaluate the water quality environment vannamei shrimp in the south of Caspian Sea (Mazandaran province) and in the Caspian Ecology Research Center. The results showed that the enrichment of water out of the ponds, can provide favorable conditions for water quality in the culture of vannamei shrimp with different densities. As a result, Mazandaran province environmental conditions (particularly temperature and salinity of the Caspian Sea) has perfectly suited for optimal growth vannamei shrimp. Therefore, vannamei shrimp (PL12) achieved a final individual weight of 21.1gr in a period culture of 85-day with a density of 35 ind/m^2. Also, The nutrients and water quality parameters (Temperature: 27.4±1.79 ° C, Transparency: 10.7±1.2 cm, Salinity: 10.57±0.78 psu, pH: 8.42±0.38, Dissolved Oxygen 8.04±1.35 mg per liter) were within normal limits during the culture period.
    Keywords: Aquaculture ; Ecology ; Environment ; Iran ; Caspian Sea ; Mazandaran Province ; Whiteleg shrimp ; Litopenaeus vannamei ; Salinity ; Physicochemical ; Shrimp
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25830 | 18721 | 2018-10-13 10:49:04 | 25830 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study aimed to assess the social, economic and fish farming in cages in the Southern Caspian Sea. The Caspian Sea is considered as one of the most important water resources of Iran, in which due to the appropriate weather conditions in the north of the country, has the potential for marine aquaculture. With the implementation of this plan would say that with respect to the proposed initial selection from Kheshtsar in Mazandaran province and Kiashahr in Guilan province with along coastline of about 250 km from the depth of 20 to 100 meters and an the area of about 2027km^2 has the cage aquaculture talent. Of course, at the depths of greater than 100 M by using the world technology procedure, there is also the possibility of the establishment of marine cage aquaculture. Now, with the regards of the above potential in the Caspian Sea coastline, it is expected at least 200 cages fish sites farming in with the establishment of 40 cages per site and produce 160,000 tons with creating of 1,000 direct jobs and 2,000 indirect jobs and income 2000 billion gross annual average net profit of 40% could be 800 billion in which will produce of proteins needed part of the country, creating jobs, welfare, prosperity and economy in the region. With the implementation of such national project, it is needed to establish pilot farms to reach the goals: environmental assessment studies, risk assessment, aquatic selection and the use of appropriate cage structures according to ecological conditions of southern Caspian Sea.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Mazandaran Province ; Guilan Province ; Cage ; Economic activity ; Profitability ; Rainbow trout ; Ecological conditions
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25833 | 18721 | 2018-10-13 10:28:58 | 25833 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study aimed to determine domain the adaptability of rainbow trout(Oncorhynchus mykiss) fingerlings in fresh water up to 20 per thousand (grams per liter) for providing facilities for growing this valuable species in the aquatic environment with salinity unconventional been completed. For this purpose the biochemical, bloody and physiologic parameters rainbow trout were studied to determine the adjustment range .Fingerlings fishes from cold water fish farm in the province Mazandran were prepared and for experiments were transferred to the Ecological Institute of Caspian Sea . Fingerlings with an average weight 31.56 ± 0. 07 SE g and average fork length 13.80 ± 0.15 SE cm, in 3 treatments in water with salinities (fresh, 13 and 20 grams per thousand) with a density of 15 numbers in polyethylene to 300-liter tank containing the 250 liters of water testing were introduced. Fresh water from Tajan rivers and water psu13 from the water Caspian Sea and water psu20 by mixing water the Caspian Sea and Sea salt was prepared . The daily amount to 50 percent of the of water tankss been replaced .During the experimental period was for 7 days and were not fed during the experiment.The water parameters was measured during the experiment included 6 ppm dissolved oxygen, pH equal to 8.2 and temperature 15.5 ° C . In the experimental period were not observed Losses in the experimental groups . The results showed that fish gill and kidney introduced in different salinities by making appropriate changes in chloride cells in the gills through increasing the number and the volume of these cells at the base of secondary blades and tubules in the kidney tubules to create greater interior space, are adapted to By changing salinity. Relatively parameters osmolarity, sodium, chloride, magnesium, cortisol, calcium, hematocrit, hemoglobin, number of red blood and white cells in water saltier than freshwater environment was higher (0.05〈 p, Duncan). Changes in hematological and blood plasma ionic parameters and vital organs Fingerlings indicates a willingness adaptability and the ability physiological adaptation fingerling was consistent with changes to environmental salinity brackish water .So, the factors measured with increasing salinity the uptrend that the range of variation for the osmolarite 449-281 mOsmol kg, for sodium, chloride, magnesium, cortisol, respectively, 211- 151, 165121, 3/3 7/0, 87. 53 mmol and the calcium 22-13 mg per dL. The measured values for hematocrit 32.2- 38.8%, hemoglobin 6.2 - 8.6 g per deciliter and the red and white blood cells, was respectively, 1.2-1.7×106 and the 15.6 -18.9×103.
    Keywords: Aquaculture ; Biology ; Iran ; Caspian Sea ; Adaptation ; Rainbow trout ; Gills ; Kidney ; Survey ; Oncorhynchus mykiss ; Fingerlings
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25839 | 18721 | 2018-10-13 10:48:08 | 25839 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The aim of this project is to study water quality and the trophic status at different months and depths in the southeast Caspian Sea-Goharbaran based on environmental parameters and nutrients during 2013-2014 in order the feasibility of fish cage culture. Results showed that water temperature, transparency, pH, dissolved oxygen, BOD5, COD, total alkalinity and TSS were varied 9.00-29.00C, 0.50-12.00 m, 8.05-8.74, 5.76-12.85, 14-101, 21-195 and 0.00-0.12 mg/l at the different months and depths, respectively. In addition, the values of NH^4+, NH_3, NO^2-, NO^3- and PO^4-3 were 0.007-0.051, 0.001-0.010, 0.002-0.015, 0.043-0.477 and 0.014-0.077 mg/l, respectively. Chl-a concentration was recorded as 0.060-8.02 mg/l. Scaled and unscaled trophic indices were equal to 3.42-5.52 and 2.61-5.85, respectively. The proper temperature for cultivation of salmonids species in this area began from October to March. The range of the standard of pH and dissolved oxygen level for fish farming cage were 7.80-8.50 and 〉5 mg /l that in current study results of pH and dissolved oxygen which were consistent in the current study. Various forms of nitrogen (ammonium, nitrite and nitrate ) at the different layers were acceptable and less than the threshold limit of Australia and New Zealand. The results of the nutrients concentrations compared with data from the reference years (1996-1996 ) in the Caspian Sea (Goharbaran region) showed that this ecosystem passed from oligotrophic status and shifted to mezotrophic and eutrophic condition. As a conclusion, although nutrients concentrations were acceptable in terms of fish farming cages in this region, some parameters such as chemical oxygen demand and transparency are not suitable for aquaculture industry. Meanwhile, based on various trophic levels, this ecosystem (Gohrabaran region) has a mezotrophic status with a high risk of eutrophication.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Goharbaran ; Physico-chemical parameters ; Water ; Fish cage culture ; Temperature ; Transparency ; pH ; Dissolved oxygen
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25838 | 18721 | 2018-10-13 10:39:41 | 25838 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Region South East of the Caspian Sea (area Goharbaran) having valuable resources biological flora and fauna, the diversity of commercial fishes, especially reserves the exclusive sturgeon as well as reserves abiotic such as oil and gas resources and transit of goods through Bandar Amirabad to Central Asia , the ecological conditions for the implementation of the project is very necessary. The aim of this study was to determine species composition, spatial and temporal distribution of plankton and fish of this region. Plankton sampled from different depths (5, 10 and 15 m) were carried out. Sampling of phytoplankton did by Ruttner and sampling of zooplankton did by net with mesh size 100 microns. The fish were sampled monthly from December 2013 to July 2014 and within months was carried out. From livestock gill Monofilament.. In this study, a total of 130 species of phytoplankton of 7 filums Bacillariophyta (60 species), Pyrrophyta (23 species), Cyanophyta (22 species), Chlorophyta (14 species), Euglnophyta (9 species), Haptophyta (1 species) and Chrysophyta (1 species )and 24 species of zooplankton branch of Copepoda (5 species), Rotatoria (7 species), porotozoa (3 species), Cladocera (9 species), and Meroplankton (2 species) of larvae of Cypris Balanus and bivalves Lamellibranchiate larvae and 256 fish Acipenser persicus at 5, Alosa braschnikowi 71 number, Alosa caspia 40 number, Benthophilius lipidus 1 number, Cluponella cultriventris 72 number, Cyprinus carpio 1 number, Liza saliens 15 number, Neogobius bathybius 1 number, Neogobius caspia 5 pcs, Neogobius flauviatilis 19 number, Neogobius gorlab 6 number, Rutilus kutum 14 number, Rutilus rutilus 2 pcs, vimba vimba 4 number was observed.Different ecological conditions appointment dietary needs and relationships of organisms and their adaptations to the environment, the density and distribution of different species of phytoplankton, zooplankton and fish specifies.Also the Caspian Sea due to the type of biological species and number of endemic species (42%) in addition to comb jelly invasion, were force of the effects some species like Gloeotrichia Echinulata and as a result, now or in the future, more species will be observed and recognized will be of most interest. Also Psedonitzschia seriata ability to produce Domick acid that can be hazardous to aquatic animals and even human, was in Goharbaran area. This was considered for fishes of the southern Caspian Sea and ecological distribution of most species depends on the region. The abundance of two species of whitefish and pelagic fish in Ghahrebaran region is more than the whole Caspian Sea. On the other hand, the dominant phytoplankton of this region is Bacillariophyta and dominant zooplankton is Copepoda, which shows the goodness of these plankton branches, and by changing the various factors as the different terms of receiving solar energy and resulting in temperature and water currents can cause seasonal differences in the density of the Bacillariophyta branch and also the Copepoda, therefore one of the most important factors is season, and in the winter, when the aquatic rotation of this ecosystem increases, it increases the nutrients and moves it from the floor to the water column, and as a result, increasing the amount of silica in various levels of water can affect the nutrition of fish.
    Keywords: Ecology ; Iran ; Caspian Sea ; Mazandaran Province ; Goharbaran ; Plankton ; Diversity ; Abundance ; Biomass ; Pelagic fishes
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    Publication Date: 2021-07-16
    Description: Mnemiopsis leidyi is one the species of comb-jelly. It belongs to the Ctenophore phylum and Lobate order. It is a purely marine, free-swimming animal with a transparent jelly-like walnut-shaped body, which was transported from the Black Sea into Caspian possibly at the end of 1990s. This species is food rival of pelagic fish. It has been reported that the ctenophore caused the dramatic decrease of zooplankton and pelagic fish stock in the Black Sea. In this study, sampling on Mnemiopsis leidyi, zooplankton, phytoplankton was conducted from December 2003 to March 2005, from a total of 11 stations located along four transects (Anzali, Khazarabad, Tourkman and Gorgan Bay) by METU net in the Iranian coasts of the Caspian Sea. Clupeonella catch was done by light fishing with fishing vessel in Anzali region. The results showed that the biomass of M.leidyi had some fluctuation in different seasons and its maximum biomass was recorded in summer with a figure of 524.4±156.2 g.m^2 Tourkman region and the minimum biomass was observed in winter with a figure of 5.15±1.81 g.m^2 in Anzali region. Study of stomach of Mnemiopsis showed, that Acartia (belonged of Copepoda with 66 %), lamlibranchia (13 %) has the highest frequency and the lowest were Balanus nauplii, Rotatoria, Tintinnopsis and Podon polyphemoides. The maximum mean abundance of zooplankton was 29368±24318 n.m3 in winter (Anzali region) and the minimum was recorded 4170±5014 n.m3 in summer (Tourkman region). Copepoda was dominant zooplankton in the Iranian coasts of the Caspian Sea. Chrysophyta with 150000000±440000000 n.m3 and Euglenophyta with 260000±521000 n.m3 had the highest and the lowest frequency respectively in the Caspian Sea. The main foods of Clupeonella were Acartia with 80 %. The survey of stomach of Clupeonella showed that the feeding of these species was not good and the highest fullness index was recorded 220.95±314.67. These results showed, the main food item of Ctenophora and Clupeonella were Copepoda (Acartia). It seems, the impact of Mnemiopsis leidyi feeding has been the important factor in declining zooplankton populations, Clupeonella stocks, and also increasing of phytoplankton abundance.
    Keywords: Biology ; Iran ; Caspian Sea ; Gorgan Bay ; Bandar Tourkman ; Khazarabad ; Anzali ; Ctenophora ; Mnemiopsis leidyi ; Diet ; Species ; Phylum ; Pelagic fishes ; Sampling ; Zooplankton ; Phytoplankton ; Acartia ; Ctenophora ; Population ; Abundance
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25254 | 18721 | 2018-09-07 07:13:59 | 25254 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Present study carried out in 6 earthen ponds (3500 m^2 each) at the Zahak fish propagation and cultivation center. In July 2005 ponds were prepared and stocked with 1100 fish specimens with 40 g average weight. Stocking normative in two ponds (1 and 4) were 220 common carp, 110 grass carp, 715 silver carp, and 55 big head specimens. In the next two ponds (5 and 6) common carp reduced to 50% and Shizotorax zarudnyi was replaced. In the rest two ponds (7 and 8) common carp reduced to zero and totally replaced with Shizotorax zarudnyi. Manure and inorganic fertilizer were used during cultivation period. Carp was fed by concentrated food and grass carp was fed by alfalfa. At the end of the cultivation period (16 months) mean weights of common carp, grass carp, silver carp, big head, and Shizotorax zarudnyi reached 1201 g, 1572 g, 313 g, 486 g, 531 g respectively. Mean daily growth were 2.41 g, 3.19 g, 0.56 g, 0.92 g, 1 g respectively. Survival rate during cultivation period were 97.57%, 68.3%, 94%, 85.45%, 46.85% respectively. Biological parameters were recorded once a month. Other chemical and physical parameters such as air and water temperatures, oxygen, pH, twice per day and factors such as nitrite, nitrate, Ammoniac, transparency, salinity and phosphate were measured and recorded once per week. During cultivation period average pH was 7-10.34, water temperature was 0.5-33 °C, transparency was 12-115cm, salinity was 1.1-5 mg/l, oxygen was 2-10 mg/l, nitrite was 0.86-0.16 mg/l, nitrate was 0.06- 2.21 mg/l, and phosphate was 0.1-1.8 mg/l. Average total yields in the ponds 1 and 4 with standard normative for the region were 554, and 623 kg. In the ponds 5 and 6 with 50% Shizotorax zarudnyi were 514 and 518 kg, and in the ponds 7 and 8 with total Shizotorax zarudnyi replacement were 533 and 386 kg.
    Keywords: Aquaculture ; Iran ; Sistan region ; Fish culture ; Poly-culture ; Shizotorax zarudnyi ; Earthen ponds ; Specimen ; Common carp ; Grass carp ; Silver Carp ; Fertilizer ; Survival rate ; Transparency ; Salinity ; Temperature ; Nitrite ; Nitrate ; Ammoniac ; Tehran ; Iran
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25258 | 18721 | 2018-09-07 07:37:38 | 25258 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Neogobius caspius is a small benthic fish that is native to the Caspian Sea. The importance of this fish is because of it is role as a main food resource of the Sturgeon fish. The genetic diversity of N.caspius populations in the Caspian Sea was studied using microsatellite technique. In the study 95 Samples of Neogobius caspius from two regions (Torkman port and Chalus) in south Caspian Sea were collected. DNA was extracted using 12 pairs of microstellite primers polymerase chain Reaction (PCR) was conducted. DNA Bands were analyzed using uvdoct and GenAlEx software package. Out of 12 microsatellite primers, 11 loci were produced, in which 5 of them were polymorphic, 6 monomorph and one of them showed smear. Average of observed and expected hetrozygosity was 0.798 and 0/843 respectively. Significant genetic differences between 2 regions was observed (P〈0.01). Deviations from hardy- Weinberg equilibrium were in all cases. These results indicate that at least, 2 population of Neogobius caspius exist in the south Caspian Sea.
    Keywords: Biology ; Iran ; Caspian Sea ; Torkman port ; Chalus ; Microsatellite ; Genetic ; Population ; Neogobius caspius ; Benthic ; Native ; Sturgeon ; Diversity ; Samples ; PCR
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25260 | 18721 | 2018-09-07 07:24:11 | 25260 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In order to study the Inheritance of Microsatellite Markers in the hybrid of Chalcalburnus chalcoides and Vimba vimba persa in the Southern part of the Caspian Sea, hybridization between the species was carried out during spring (2009) in Shahid Ansari reproduction and culture workstation. 30 samples from the produced larva and also small piece of dorsal and pectoral fin of their parents were collected and stored in pure ethanol (96%) and transferred to the biotechnology of the Caspian Ecology Academy. DNA was extracted from fin samples of the parents, whilst the whole F1 progeny was used to isolate the DNA by Phenol-chloroform method. Quantity and quality of extracted DNA was determined using 1% Agarose electrophorus. DNA was used for PCR ampilification of 10 microsatellite loci. The amplifications were run on 6% Polyacrylamid electrophorus. The present study showed that 7 out of 10 markers were polymorphic and variated between the parents. Goodness of fit to the Mendelian inheritance ratio was determined by X2 analysis. 4 primers were segregrated according to Mendelian expectations at a significance level of P 0.05. Based on UPGMA dendrogram (Nei, 1978) two different clusters were obtained in which Chalcalburnus chalcoides and hybrid were in the same cluster but Vimba vimba persa was in the other. It seems that Vimba vimba persa is a joint ancestor for these two. High heterozygosity presence in parents and their F1 shows the high potential of genetic variety in Chalcalburnus chalcoides and Vimba vimba persa which from the aquaculture management and conservation view is important and considering the high genetic variety in their hybrid, the hybrid has the sufficient potential for race improvement.
    Keywords: Biology ; Iran ; Caspian Sea ; Chalcalburnus chalcoides ; Vimba vimba persa ; Hybrid ; Microsatellite ; Hybridization ; Culture ; Samples ; Larvae ; DNA ; Inheritance ratio ; Heterozygosity
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25261 | 18721 | 2018-09-07 07:21:13 | 25261 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Report of the project entitled "determination of the global population structure of the great sturgeon (Huso huso) with emphasis on the Golestan Province" is in front of your attention. Outcome is the results of study on the samples collected from the total catch in the landing sites namely Bandar-e-Anzali, Babolsar, and Ashooradeh, in the three Northern provinces. 224 specimens were collected according to the sampling manual during the fishing seasons in 2005 and 2006. Three methods were applied in this study to find out the population structure of the great sturgeon in the southern Caspian Sea. These were as follows: Morpho-metric meristic Population dynamics Population genetics (microsatellite loci) Results indicate that the range of different age groups of the great sturgeon in the Golestan Province is larger than the other two provinces. Results show that the older age groups are found in the samples from the Golestan Province rather than the other two provinces. Results demonstrate that the sample size for the two methods (morpho-metric meristic and population dynamics) is not enough. In order to obtain precise results with less uncertainty one needs to collect more specimens. Therefore, according to the few number of the great sturgeon in the total sturgeon landing, it is recommended that researchers apply methods other than the two methods which are mentioned above in order to make sure that the results are most reliable. Population genetics methods including microsatellite loci are among the methods which could produce good results with minimum bias in this regard. Results indicate that there are two distinguishable populations of the great sturgeon (Huso huso) in the southern part of the Caspian Sea off the shores of the three Northern provinces. Results also show that these two populations are scattered in the two east end (Golestan Province) and west end (Guilan Province) of the southern Caspian Sea. The two populations have overlapped each other in the Mazandaran Province.
    Keywords: Biology ; Iran ; Caspian Sea ; Golestan Province ; Guilan Province ; Mazandaran Province ; Babolsar ; Bandar-e-Anzali ; Population ; Sturgeon ; Huso huso ; Sampling ; Morpho-metric ; Samples ; Meristic ; Genetic ; Microsatellite ; Specimens
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25270 | 18721 | 2018-09-07 07:54:06 | 25270 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Sampling of Astacus leptodactylusc were conducted in order to determination of biometrical and biological parameters suchas length, weight, sex ratio, fecundity and natural reproduction time. Two transect were selected at 49 34 and 49 36 geographical position on east Caspian Sea near to Anzali city. Metallic folding trap with Silurus glanis as attractive diet were used to catch Astacus leptodactylusc at each line the traps were set on depth of 35, 45, 55 and 65 (5 trap at each depth). Random sampling from each depth on tow lin for one year were conducted and the biometry performed on catched Astacus leptodactylusc where their sext uality and their ration were determined for eacd month , season and whole year. Absolute fecundity determined by cooking Astacus leptodactylusc , taking out the ovary weighing and counting them .Working fecundity assesed by separating eggs from their swiming leges while enomerate all egg . Complete randomized test of ANOVA for analysing the data were employed. The results showed average length and welght were calculated 122/07±1/74mm and 57/96±1/81gr respectively. Average absolute fecundity was 310/22 ±10/72 eggs , average working fecundity was 251/84±8/84 eggs, Average ovary weight was determined 4.31±0.619 gr and average number of eggs in one gr was 74/52±1/53 eggs. The sextual ratio in all year long was 1:1.32. The reproduction season is about seven month from Feburary to July and the moulting of males occurs two times each year. One of time is at May and the other is in September. The female molt thtina as the male start for second time.
    Keywords: Biology ; Iran ; Caspian Sea ; Bandar Anzali ; Biology ; Astacus leptodactylus eichwaldi ; Sex ratio ; Fecundity ; Silurus glanis ; ANOVA ; Female ; Male ; Coastal water
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25272 | 18721 | 2018-09-07 08:25:12 | 25272 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This experiment conducted to evaluate the effect of different dietary and bed treatments on earthworm (Eisenia fetida) for aquaculture. Concurrent with initial proliferation of earthworms, a pilot experiment also performed. In pilot experiment that conducted to become familiar with specialty and requirements of Eisenia fetida earthworms, lasted 4 months. The pilot treatments were including: 1) cow manure with covering its surface (control group), 2) cow manure (C.M.) without covering its surface, 3) C.M.+ 5% wind sand, 4) C.M.+30% saw dust, 5) urban compost, 6) C.M.+20% alfalfa meal, 7) C.M.+ 20% wheat bran and 8) C.M.+20% rice bran. After initial proliferation of earthworms and performing a pilot experiment, two trials designed. The first trial performed in outdoor environment, inside the plots dugged in the earh, with six treatments. The treatments were including: 1) cow manure(control group), 2) cow manure (C.M.) with vertical ventilation, 3) C.M.+ 30% saw dust (S.D.), 4) C.M.+30% S.D. with vertical ventilation, 5) urban compost+20% C.M. and 6) urban compost+40% C.M. The experiment lasted 6 months. In the end of trial in every plot, from 4 points, sampling for number of mature and immature earthworms, cocoons and the weight of earthworms were measured. The second trial performed in greenhouse by using wooden boxes. In this trial the treatments were including: 1) C.M. as control group, 2) C.M.+30% S.D., 3) C.M.+30% S.D.+ 10% alfalfa meal, 4) C.M.+30% S.D.+10% wheat bran, 5) C.M.+30% S.D.+10% rice bran, 6) C.M.+30% S.D.+10% soil, 7) C.M.+30% S.D.+5% fine sand, 8) C.M.+ 30% S.D.+1% lime powder and 9) C.M.+30% S.D.+200 ppm ammonium nitrate. Every treatment performed in 3 replicates. The earthworms reared in controlled condition for moisture and temperature, for about 4 months. The pH of treatments measured monthly by paper pH meter and by method of solution extraction. In the end of trial the number of mature and immature worms, cocoons and the weight of earthworms in every box were measured and the average weight of every earthworm calculated. The results of pilot experiment showed that covering surface the bed is necessary for maintaining moisture and darkness for earthworms. In wind sand treatment, the bed had sticky and compacted statues and appeared that the earthworms moved and activated difficultly. The saw dust treatment had a wet- spongy environment and the earthworms distributed well in it and their size was larger than control. Urban compost treatment unlike its suitable pH (equal 8), hardly accepted by the earthworms and they stayed in the corner of bed for more than one month. The wheat bran and rice bran treatments, both had sticky texture at the beginning but by spending time, their beds improved. The results of first trial showed the Eisenia fetida earthworms can survive and growth in outdoor environment even in cold season of winter. In this trial the highest total number of eartworms and cocoons and the total weight of earthworms was belong to control group, that had significant difference with the treatments C.M. + 30% S.D., urban compost + 20% C.M. and urban compost + 40% C.M. (p〈0.05). The performing of ventilation in experimental treatments had not significant effect on the number of mature earthworms, total number of earthworms and their total weight compare to control group. The urban compost was not suitable for rearing Eisenia fetida, although addition of 20 and 40 % of cow manure could improve it for usage by the earthworms. The results of second trial showed that, addition of alfalfa powder, wheat bran, rice bran and lime powder to cow manure had not significant effect on the total number and total weight of earthworms compare to control group. The usage of soil in treatment C.M. + 30% S.D. + 10% soil caused significant decrease in number of mature earthworms compare to control group (p〈0.05). The treatments including addition of saw dust and saw dust + fine sand to cow manure increased the number of mature earthworms, cocoons and total weight of earthworms compare to control group significantly (p〈0.05) and treatment of ammonium nitrate caused significant increase in total weight of earthworms (p〈0.05) .
    Keywords: Aquaculture ; Iran ; Diet ; Treatment ; Earthworm ; Eisenia fetida ; Aquaculture ; Dietary ; Ammonium ; Nitrate ; Temperature ; pH ; Mature ; Immature ; Growth
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25276 | 18721 | 2018-09-07 07:59:49 | 25276 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Following to introduce Vannamei shrimp (Litopenaeus vannamei) into Iran aquaculture industries by fisheries organization its postlarvae were successfully reared in ponds in Bushehr and Choebdeh in Khuzestan. Due to activity of Shrimp site and importance of input and output water quality, this study was done in 11 stations including: two stations in Bahmanshir river, two station in C4 and C5 irrigation canals, six staions in active farms along C4 cannel (Yonesi, Mosavei and Salman Zadeh), and C5 cannel (Mohamadi, Khairi and Ashraf Por) and one staion in output draing were selected. Sampling were carried out from May2009 befor beginning stocking to harvest time in October 2009. Physico- chemical parameters and plankton sampled biweekly and benthic animals and heavy metals were sampled monthly. Some parameters such as pH and temperature by using portable multy parameters and others. Analyzed with standard methods. Heavy metals were measured by plarography method (797 Vamtro). Plankton and benthos samples were studied only in Bahmanshir station. According to results the maximum values for DO (11.1 ppm) PO4 (1.86 ppm) NO3 (8.4 ppm) and TSS (4992 ppm), pH (8.4) NO2 (0.18 ppm) BOD5 (9.06 ppm) were measured. In comparison to aquaculture water quality standards except for TSS in river stations others are located in allowable range. We didn’t observed any main changes in water quality from river to output drainage. The results of biotic parameters in river stations showed that diatoms were the most group of phytoplanktons. Zooplankton groups were Copepods, Rotifera and Protozoa. Tintinids and Nematoda larvae were the most abundant zooplankton. Benthic animals were included nine crustacean species and two annelid species. Isopods with 52% were the most and ant group.
    Keywords: Aquaculture ; Ecology ; Iran ; Khuzestan Province ; Abadan ; Choebdeh ; Bahmanshir River ; Vannamei shrimp ; Litopenaeus vannamei ; Water quality ; Abadan aquaculture site ; Rearing ; Shrimp ; Ponds ; Sampling ; Plankton ; Heavy metals ; pH ; Temperature ; Benthos ; Phytoplankton ; Zooplankton ; Copepods ; Nematoda larvae
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    Publication Date: 2021-07-16
    Description: Rutilus frisii kutum is one of the important fish of Caspian Sea which has significant economical role in the region .The objective of this project is a comparative study on traditional culture of fishes versus slurry. This survey were conducted on nine pound of 1.7 hec triplicate with their treatment with stocking densities of 1.7 million fish larva at Dr yousefpoor center (Affiliated of Shahid Beheshti culture and propagation) at Siakal village , 32 km far from Rasht city in North of Iran, the first treatment considered as control which practice traditionally (using concentrated food plus grinded kilka fish) .In the second treatment for 13 days the pound supplied only by slurry then followed by concentrated fish food only .The tired treatment started with slurry for 13 days and followed by slurry plus concentrated food. The physical and chemical parameters of water, plankton, biometry of fish, growth indices such as daily weight growth (DWG), daily length growth (DLG) as well as specific ratio (SGR) were monitored weekly. The result indicate that net fish production was 1.7 to 2 times higher in slurry than traditional treatment also the survival rate were 1.7 times higher in slurry treatment . The treatment of slurry follow by addition of concentrated food plus slurry showed higher yield camper to others. Slurry with several active substances is more effective and can promote the growth of zooplanktons which is the food of larval stage of rutilus frisi kutum. In second phase of this project the effect of slurry was surveyed on Chinese carp. This experiment was conducted in triplicate with two treatment (one treatment is control which use cow manure plus chemical fertilizer and second treatment was conducted with slurry) and stocked with 2375 N/hec (Silver carp 60%, Big head 15%, common carp 17% and grass carp 8%) at June. In this survey common carp and grass carp were feed with concentrate food and fresh grasses respectively. Chemical factors were measured once for each two week and density of plankton was determined when it was necessarily. Nutritional content of phosphorous, nitrogen, calcium, potassium and magnesium after anaerobic fermentation as well as protein and lipid content with 1.4and 1.9 times respectively were higher in slurry treatment than control. The results showed that survival rate and yield were higher in slurry than control. The increasing percent of yield were 13.5, 2.6, 18.4 and 85.3 in Silver carp, Big head, common carp and grass carp respectively. The survival rate of grass carp was twice higher in slurry treatment than control. Zooplankton abundant in slurry pounds was more but blue- green algae density was less than control. Protein and lipid analysis reviled no significant difference between control and slurry. In general the result indicate that the slurry with higher nutritional content is more effective on the survival and growth rate of fishes and also is more efficient in proliferation of plankton in particular zooplankton.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Slurry ; Rutilus frisii kutum ; Cow fertilize ; Larviculture ; Carp ; Culture ; Plankton ; SGR ; Zooplanktons ; Silver Carp ; Big head carp ; Common carp ; Grass carp ; Phosphorous ; Nitrogen ; Survival rate ; Protein ; Lipid
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25304 | 18721 | 2018-09-08 16:49:09 | 25304 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The Common carp is the most important culture fish in the word, that can be consider as domestic fish. The Common carp of Caspian Sea is one of the economic fish, that have some morphenological differences, resistance in salt water and better quality in meet with domestic once and for using this fish in culture system, it is necessary to establish genetically parameters especially the heritability for selection purpose. The present study have been started from 2007 and lasted 3 years. The breeders were collected from the Gomishan station in south-east of Caspian Sea by fisherman and stocked in S.Rajaei fish restocking center and propagate based on the routine propagation technique in this farm. For heritability estimation a factorial crosses by 7×7 fish were done. Therefore, the fingerlings of 49 crosses were achieved, that kept first separately fiberglass tank and then in pond separately. Weight and length of fish were choice for heritability estimation. The average weight of juvenile common carp in 3 sampling stage of 90, 180 and 360 days, were 5.9, 35.7 and 84.7 gram and length of 9.6, 14.7 and 19.2 cm respectively. The heritability estimation for weight was 0.26 and length 0.25. Selection response based on selection difference by collection the more average breeders among their progeny were 36.4 g that means by selection every generation, there is about 4 percent improving in fish weight.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Gomishan ; Common carp ; Cyprinus carpio ; Heritability ; Selection Response ; Culture ; Weight ; Length ; Fish ; Sampling ; Generation
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25319 | 18721 | 2018-09-11 09:09:09 | 25319 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Grey mullet, Mugil cephalus is one of the most famous and valuable fish on the fisheries and aquaculture industry. This species is the most suitable to introducing the southern, northern and central regions of our country due to high resistant to large variation of water salinity and temperature. On the monthly sampling period biometric data including total length and total weight recorded and length and weight classes for every sex were determined. To understanding of physiological functions and sexual development of reproductive organs, this investigation carried out with Grey mullet imported from Hong Kong by 1993. Findings of this research can be used for management of artificial breeding of this species. Daily variation of pond water temperature was recorded. Sex steroid hormones and other plasma metabolites such as Ca^2+, cholesterol, triglycerides and total proteins were measured by blood sampling. Gonad samples were prepared in order to histological studies. Oocyte growth was studied by measurement of egg diameters. Measured values of cholesterol, triglycerides of blood plasma revealed that these metabolites have seasonal variation and severely deposited on the summer and mobilized on the spawning seasons. Findings of this research suggested that egg diameters, values of sex steroid hormones (estradiol and progesterone) and Calcium concentrations of fish blood plasma shortening increased a long with day length shortening.
    Keywords: Aquaculture ; Iran ; Golestan province ; Mugil cephalus ; Sex steroid hormones ; Plasma metabolites ; Gonads ; Gray mullet ; Mugil cephalus ; Fisheries ; Aquaculture ; Species ; Salinity ; Temperature ; Sampling ; Estradiol ; Progesterone ; Calcium
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25326 | 18721 | 2018-09-12 10:09:55 | 25326 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Recently, the ecology of the Caspian Sea coastal has undergone with increasing development of human activities and industrial wastewater discharge. Therefore, considering the current situation, evaluate of pollutants issue in the Caspian Sea is very important. Results of this study showed that levels of heavy metals (Cd, Cr, Cu, Fe, Pb, and Zn) of water had lower than standard and also acceptable for aquatic life. Significant accumulation of metals such as Co, Cu and Cr in the sediment was observed at transect Sefidrood and Anzali in comparison with six other transects (Turkmen, Amirabad, Babolsar, Noshar, Tonekabon and Astara) and for Ni at transects Sefidrood and Astara compared with six other transects (Turkmen, Amirabad, Babolsar, Noshahr, Tonekabon and Anzali) (P〈0.05). The amount of Zn in fish muscle Liza saliens was maximum and then metals such as Fe, Cu, Pb, Cd, Ni, Hg and Co were recorded (P〈0.05). In the north of Iran, two kind of bony fish, such as R..frisii kutum and Liza.s have maximum catchments as compared with other bony fish in the Caspian Sea. Based on calculation of risk of Target Hazard Quotient (THQ), we obtained THQ was less than one for all metals in young and adults human. Thus, consumption of R..frisii kutum and Liza.s per capita with 6 kg will not be at the risk for young and adults. Among of oil derivatives, polyaromatic hydrocarbon (PAHs) is hazardous compounds in the environment. Environmental Protection Agency (EPA) was announced 16 compositions of PAHs as an index. The results of this project showed that concentration of two and three rings of PAHs was lower than 4, 5 and 6 rings of water, sediment and fish at most stations and seasons. Among of 16 PAHs, only Benz(α)anthracene, chrysene, Benzo(α)pyrene and Dibenz (α, h)anthracene compounds were contained with ecological risks (HQ) more than one in the water and sediment. In addition, three compounds such as Chrysene, Benz(α)anthracene and Benzo(α)pyrene were observed in the edible fish tissues (Liza.s and R..frisii kutum) which have high ecological risks in the water and sediment. During four seasons (spring, summer, fall and winter), maximum concentration of organochlorine residue in the surface water (5m depth) were belong to β-Endosulfan (Turkmen), Dieldrin (Babolsar, Tonekabon) and endosulfan sulfate (Astara) and at 10m depth were γ-BHC, Dieldrin compounds (Tonekabon), DDT (Turkmen) and Aldrin (Babolsar). This values at 50m depth were belong to γ-BHC (Anzali), δ-BHC (Tonekabon), Dieldrin (Amirabad), and heptachlor (Amirabad). Maximum percentage of organochlorine pesticides residue in the sediments was belonging to only Aldrin compound at stations Turkmen, Babolsar and Tonekabon. In addition, maximum concentration organochlorine pesticides residue in fish tissue (Liza.s and R..frisii kutum) was DDT and Endrin aldehyde compounds. Mean surfactant concentration (LAS) of surface water (5, 10 and 50 m depths) at eight transects was 0.017±0.049 mg/l. The maximum concentration of LAS were 0.084 mg/l and 0.082 mg/l in spring and summer (Anzali transect), respectively. This value was 0.035 mg/l and 0.060 mg/l in autumn and winter (Sefidrood transect), respectively. As a conclusion, the concentration of surfactant in the Caspian Sea basin is not critical as compared with standard level.
    Keywords: Pollution ; Iran ; Caspian Sea ; Anzali ; Babolsar ; Astara ; Noshar ; Sefidrood ; Survey ; Pollutants ; Heavy metals ; Hydrocarbons ; Aquatic ; Bony fish ; R.frisii kutum ; Liza ; PAHs
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25329 | 18721 | 2018-09-13 12:35:03 | 25329 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This project were investigated seasonally from march 2008 up to one year, in southern Caspian Sea iranian coast /Sampling was carried out at 8 stations of between 5 and 100 m depth and taken in triple with a 0/1m² van Veen grab/Total twenty four species and one family and one order were identified, presenting low dominance and frequency/ polychaeties 56%,oligochaetes 39% bivalves 〈1% amphipodes1% Cumacea and diptera and cirrepedia %1 of whole macrobenthos compositions/ Polychaetes and oligochaeta dominated, representing 95% of the whole fauna/Average of species were 11/8 species per grab/max 21 species / grab were found in Tonkabon stations at 100 meter depths and minmum 4 species/grab in 20 m in Bandar anzali /Maxmiun density were 4783 n/m^2 in spring and minmum 3177 n/m^2 in winter/Highest density were recorded in Amirabad stations and lowest in nooshar/ Maximum density showed in east, mimun in middle area of sea The biomass of benthos adventages in west/Average biomass of whole area were 6/932 gr/m^2/Mean total organic matter were 3/11±1/1 and silt- clay 62/92±6/12precent and Mean dissolve oxygen and salinity were 8/3±0/15 mg/l and salinity 12/2 ±0/4 ppm and water tempreture were 14/9±0/18ْC/ Mean grain size, fine sand, very fine sand, silt and clay contents were among the main factors related to the patterns of macrofaunal distribution, density and diversity and dominance of trophic groups.
    Keywords: Ecology ; Iran ; Caspian Sea ; Tonkabon ; Bandar Anzali ; Macrobenthos ; Disturbiotion ; Diversity ; Productions ; Survey ; Diversity ; Abundance ; Biomass ; Macrobenthic Fauna ; Sampling ; Species ; Polychaeties ; Oligochaetes ; Bivalves ; Dissolved oxygen ; Salinity ; Temperature
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25335 | 18721 | 2018-09-13 12:35:23 | 25335 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was conducted to study of Hydrology, Hydrobiology and Environmental pollution in the southern of the Caspian Sea. 33 stations were selected from Astara (in the west) to Hassan gholi (in the east). In each station, sampling carried out at suface, 10, 20, 50 and 100m depths. Sampling was performed during four seasons in 2004. Sampling stations also divided into three regions, including west (from Astara to Ramsar), middle (from Ramsar to Sari) and east (from Amirabad to Hassengholi). The results showed that maximum of water temperature was 24 ?C at summer in the eastern region while the minimum of water temperature in winter season has been 9.7 ?c in the western region. The average of dissolved oxygen concontration in spring, summer, autumn and winter season were 6.9 , 6.6, 8.2 and 6.7 mg/l , respectively. The maximum value of salinity was observed in spring (13.55 ppt) and minmum value in winter (8.24 ppt). The average of total nitrogen concentration were season was 711, 884, 583 and 669 µg/l in spring, summer, autumn and winter repectively. The average of total phosphorus concentration was 27.9, 37.1 and 42.6 µg/l in spring, summer, autumn and winter, respectively. The average of dissolved silica concentration has varied from 167 to 923.6 µg/l. The five phyla of phytoplankton have identified. The most species diversity was observed in winter (70 species) but the low species diversity has obtained in autumn. The most phytoplankton population was in spring and summer seasons. The cell abundance of phytoplankton in different seasons was fluctuated from 3914*103 to 17337*103 ind/m^3. In addition, the phytoplankton biomas was varied 11.4 to 30.7 mg/l. Result showed that distribution of phytoplankton are varied at different areas, layer, seasons and stations. The average of cell abundance of zooplanktons was varied from 2023 to 12364 and/m^3 at different seasons. The mean of bioman varied from 9.40 to 43.48 mg/l. In this study, 17 species and one class of macrobenthos were identified. The frequency of Annelida and Biovalvia was higher than all macrobenthos. The average of macrobenthos was 1218±851 ind/m^2 and their biomass was 14±15 g/l. The average of PAHs concentration were 0.038, 0.028 and 0.21 mg/l in spring , summer and winter, respectively. The decending trend of heavy metal concentration were Fe〉Mn〉Zn〉Cr〉Pb〉Co〉Cd〉Cu at different seasons in 2004. The maximum concentrations of organochlorine pesticides (OCPs) components in three regions of sampling zone such as , -BHC (middle), β,-BHC (middle), DDT (east) and dieldrin (east part) were 88.3, 35.0, 74.3 & 79.1 g/l during spring, summer, fall and winter, respectively.
    Keywords: Ecology ; Iran ; Caspian Sea ; Astara ; Physo-chemical parameters ; Plankton ; Benthos ; Enviromental Pollutions ; Hydrology ; Hydrobiology ; Sampling ; Temperature ; Dissolved oxygen ; Dissolved silica ; Species ; Phytoplankton ; Biomass
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25334 | 18721 | 2018-09-12 09:56:40 | 25334 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Distribution of freshwater shrimp (Macrobrachium nipponense) in the freshwater ecosystems such as Alagol, Almagol, Ajigol lagoons, Gomishan lagoon and Gorganrud River. Sampling were done from may 2009 to my 2010 parameters such as, length frequency, growth rate, sampling, feeding, environmental factors and sedimentation were investigated. 3689 females and 8551 males were collected and biometrical parameters were recorded. Total length ranged between 3.2 to 98.7 mm. The parameters b of length-weight relationship in Alagol, Almagol, Ajigol were 3.49, 3.15, 3.45, 3.22 and 3.22, 3.28 for male and female, respectively. It shows in all lagoons for male and female growth is positive Allometric. Spawning period was from May to June. The length first at maturity (total and/or carapace) for three lagoons was 50.5 mm and 133 mm, respectively. The minimum and maximum of egg volume were 0.12 mm3 in Ajigol and 0.07 mm3 Alagol, respectively. The relationship between fecundity and length was found to be linear regression models for Alagol, Ajigol and nonlinear regression models for Almagol lagoon. Result showed that this species is omnivorous. The minimum and maximum temperature was 6.5ْ C in fall and 33ْ C in summer. The lowest and highest fluctuations of pH level in Ajigol and alagol lagoons were 8.2-9.2 and 7.2-9.8. Among three lagoons, the most EC was 5.9 Ms/c in Alagol lagoon. The Gorganrud River shows the maximum EC, 16.7 Ms/c.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Golestan province ; Alagol lagoon ; Almagol lagoon ; Ajigol lagoon ; Freshwater shrimp ; Macrobrachium nipponense ; Wetland ; Biology ; Ecosystems ; Sampling ; Growth ; Eggs ; Identification ; Distribution
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25353 | 18721 | 2018-09-14 07:27:04 | 25353 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Gillan , Mazandaran and Gorgan provinces have temperate climate, thus they have more potential for aquatic animal culture. There are thousands hectare of lands for Aquaculture and fisheries in adjacent to Caspian Sea in this provinces. These areas (North alborz) have provided a favorable back ground for aquatic animal breeding and stock enhancement of species in the sea. As a result, this investigation executed during two stages (phases). At first stage, the goal of this project included the feasibility study and demonstrating the existing situation of fish stock enhancement activities in North Alborz area and second phase is to survey on distribution, and production activities of stock enhancing hatcheries as well as their constructions, and production time table. Survey was started from 2006 to 2008 by using qestionaries in 7 hatcheries from tree provinces. Results reviled that there are more than 10 species was subjected to restocking .Total releasing was about 300 millions of fry and finger lings in to the Caspian sea.
    Keywords: Management ; Iran ; Caspian Sea ; Guilan Province ; Mazandaran Province ; Gorgan ; North Alborz ; Stock Enhancement ; Broodstock ; Production ; Yield ; Artificial propagation ; Bony fishes ; Sturgeon fishes ; Restocking ; Aquatic ; Culture ; Aquaculure ; Fisheries ; Breeding ; Survey ; Hatcheries ; Fry ; Fingerlings
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25363 | 18721 | 2018-09-14 07:46:07 | 25363 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This project was carried out in order to determine the hatching conditions and laboratory culture methods of Fairy Shrimps (Phallocryptus spinosa). Physico- chemical factors and phytoplankton of natural habitat of this organism was investigated in Khasellou region around Azarshahr – East Azarbaijan province. The results showed that occurance and growth of fairy shrimps in their habitates were beginning from early Aprill and their survival was decreased with increasing temperature, precipitation and increasing the salinity of their culture medium. Then they disappeared. Their habitat phytoplankton contained 3 phylla and 10 genera, from which green algae enjoyed the greatest density in May. The pool salinity was around 20-17 g/l, when metanauplii was observed in early spring. In the pool containing and without fairy shrimps the oxygen concentration was high (9 mg/l) and low (2.9 mg/l), respectively. In 9 prawn ornamental fish genera, feeding with fairy shrimp showed a significant higher fecundity and hatching percentage compared to manual diets (cow liver, spinach). In all groups fed with fairy shrimp the duration of spawning time were reduced from 15 to 8-10 days and the color was enhanced. The means of cyst number per captured female also, cyst, decapsulated cyst and Naupilli diameters were mesured 142.9±19.0 cysts, 273.2±4.9µ, 242.4±3.8µ and 542.6±27.0 µ, respectively. The hatching rate was increased by reducing temperature and salinity of their culture mediums from 25 to 15 ◦C , and 28 to 18 g/l, respectively. The highest hatching was observed on 5th day at 15 ◦C and 18 g/l (5, 33 %). In addition, low but multiple hatching was observed. Therefore, water temperature and salinity affected hatching rate in this species. The hatching success of Phallocryptus spinosa cysts was better when collecting cysts together with dry sediments of their habitat without separation sensitive cysts. In addition to cold keeping and freezing, the hydration – dehydration method caused more diapause deactivation for hatching induction. The laboratory results revealed that the survival of fairy shrimps were highest at 15 ◦C (42%) and lowest at 25◦C (26%), respectively. Also, their life was inhibited in 30◦C. In different salinities, the mean survival rate were 26% (Salinity= 15 g/l) and 20% (Salinity= 25 g/l), respectively. The mean survival percentage of Phallocryptus spinosa cultured in natural habitat water (64.3%) was higher than aerated tap water (23, 3 %). Also, feeding with Nanochlorepsis caused higher survival rates. On the other hand, Beakers yeast caused higher mortality rates. Therefore, regarding to relatively high sensitivity of these species to physico– chemical and nutritional conditions of their culture medium, it is better to culture these species with expansion of their natural habitat in order to preserve their biodiversity, culture and increase stocking density with regard to environmental considerations .
    Keywords: Aquaculture ; Iran ; East Azarbaijan province ; Fairy Shrimps ; Phallocryptus spinosa ; Hatching ; Culture ; Biotechnique ; Species ; Anostraca ; Phytoplankton ; Growth ; Temperature ; Salinity ; Phylla ; Feeding ; Survival rate ; Mortality ; Biodiversity
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25371 | 18721 | 2018-09-14 07:25:56 | 25371 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: At the present study, the environmental pollutants such as organochlorine pesticides (OCPs), heavy metals, hydrocarbons and surfactants were done during 22 months (Sept. 2009 through May 2011) located in southern part of Caspian Sea with longitude and latitude 48°-54° N and 36°-39° E, respectively. The aims of this study were to determine the seasonal pollutants matters in water layers and bed sediments of eight transect (24 stations) and the results are as follow: The maximum seasonal percentage range of OCPs were detected in spring water samples from 10, 50, and 50m depths such as (DDD, δ-BHC, heptachlor epoxide, endrin aldehyde),(DDD) and (aldrin, β-endosulfan) compounds about 62.5, 75 and 100%, respectively. The maximum seasonal residues fluctuation of OCPs were determined in spring water samples from 10, 50, and 50m depths such as aldrin (Babolsar station), aldrin (Tonekabon station) and heptachlor epoxide (Astara station) compounds about 5.03, 3.08 and 31.43 µg/l, respectively. The maximum percentage range of OCPs were detected in sediments samples from 10, 50, and 50m depths such as aldrin and α-BHC (winter), α-BHC (summer and winter) and aldrin (summer) compounds about 100, 75 and 87.5%, respectively. The maximum residues fluctuation of OCPs were determined in sediments samples from 10, 50, and 50m depths such as α-BHC (summer in Nushahr station), α-BHC (summer in Sefidroud station) and α-BHC (winter in Tonekabon station) and compounds about 5.96, 3.77 and 3.07 µg/l, respectively. The fluctuation and distribution of Total Petroleum Hydrocarbons (TPHs) concentration in different water layers samples were reduced from summer〉spring〉 fall 〉 winter, respectively. Also this trend occurred for bed sediments and reduced from winter 〉 summer, respectively. The mean concentrations of TPHs in water samples of all seasons, regions, depths and transects were less than maximum permissible concentration (MPC). In this research, a comparison of TPHs with EPA standards shown that the desile range organic (DRO) was close to EPA standards such as TPHs and also 95 percent of water data were less than MPC. But gasoline range organic (GRO) concentrations in all stations were less than the amount of EPA standard. A comparison of TPHs concentration in sediments shown that the concentration of all stations were less than of national research council (NRC) range except west part. The maximum annual mean concentrations of Hg and Pb elements were detected in surface station (50m) at Nushahr and Amirabad transects. The most water data of Cd, Pb and Hg elements in comparison with critical concentrations with Europe, the USA and Japan standards were less than amounts of those standards. The distribution and abundance of Cd, Pb, Hg and Ni elements in water samples were detected 98, 96, 77 and 6%, respectively less than the ISQGs (Interim marine sediment quality guidelines) standards. In sediments samples, the mean and maximum concentration of Hg element detected in winter in comparison with ISQGs standards was more. But the concentrations of Cd and Pb in sediments samples of all stations were low and less than of ISQGs standards. The maximum concentration of linear alkyl benzene sulfonate (LAS) from spring through winter in Anzali (spring), Tonekabon (summer), Anzali (fall) and Nushahr (winter) were 0.07, 0.45, 0.145 and 0.087 mg/l, respectively. The maximum concentrations of LSA were detected in spring and fall in west part and summer and winter in middle part. But the lower concentration was occurred in west of southern part of Caspian Sea. According to standards of surfactants and comparison with LAS concentration of this study were less than the critical points.
    Keywords: Pollution ; Iran ; Caspian Sea ; Babolsar ; Tonekabon ; Astara ; Organochlorine Pesticides (OCPs) ; Total Petroleum Hydrocarbons (TPHs) ; Heavy Metals ; Surfactants ; Environmental Pollution ; Hydrocarbons ; β-endosulfan ; Sediments ; Samples ; Alkyl Benzene Sulfonate
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25385 | 18721 | 2018-09-18 05:58:09 | 25385 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study ichthyoplankton, phytoplankton, zooplankton, benthic and hydrochemistry parameters were studied. 9 out of 11 sampling stations were in the Gorgan Bay area and 2 of them were in the mouth of the Bay. Ichthyoplankton survey were carried out in 6 stations and the other surveys such as phytoplankton, etc were done in 11 stations in 2009. Gorgan Bay map and position of sampling sites are given at the end of material and methods section. Biomass and frequency of phytoplankton at different stations, months and seasons were calculated that are presented in Tables 1 to 3. Hydro-chemical parameters such as water temperature, air temperature, visibility, DO, BOD5, pH, Alkalinity (bicarbonate, carbonate and total), Hardness (calcium, magnesium), CL,EC, Salinity, N (nitrate, nitrite, NH_3), Phosphate were measured and results are shown Table 11. Biomass (mgr/m^3) and numbers (ind./m^3) of 47 genus of different phylum of phytoplanktons such as Cyanophyta, Chlorophyta, Pyrophyta, Chrysophyta and Euglenaphyta as well as different phylum of zooplanktons consist of Protozoa, Arthropoda, Rotifera and Cyliophora were estimated. Frequency of phytoplanktons (ind/m^3) was 131.2, 1.6, 65.3, 18.9 and 23.8 million respectively. Furthermore, their biomass was 308.4, 1.9, 1358.9, 295.1 and 124.7 mg/m^3, respectively. The maximum and minimum numbers were observed in Leptolingbaya belonged to Cyanophyta and Diatoms of Chrysophyta 84.3 and 0.025 million (ind/ m^3) respectively. The maximum and minimum biomass was observed in Gyrosigma (Chrysophyta) and Tetradron (Chloropyta), 12317.3 and 0.014 mg/m3 respectively. The average biomass of zooplankton was measured 531.74, 30.77, 225.07, and 96.26 mg/m^3 respectively and the total was attained 883.83 mg/m3 Table 5. The maximum and minimum biomass of all zooplanktons was estimated in November and July, 3446.23 and 6 mg/m^3 respectively. Furthermore, the maximum and minimum numbers of zooplanktons were found in September and July which were 48.4 and 0.002 millions respectively and the average number was 8.4 ind/m^3 Table 4. Also numbers and biomass (gr/m^2) of 11 families of benthos which inhabit Gorgan Bay such as Annelida, Mollusca and Arthropoda were investigated in separated stations and distinctive months. The maximum and minimum biomass of benthos was observed in January (42.91 gr/m^2) and March (0.6428 gr/m^2). The biomass was considerable in April, February, August and December (Tables: 4, 5 and 6).
    Keywords: Ecology ; Iran ; Caspian Sea ; Gorgan Bay ; Ecological study ; Fisheries ; Ichthyoplanktons ; Phytoplankton ; Zooplankton ; Benthic ; Sampling ; Biomass ; Temperature ; Visibility ; pH ; Alkalinity ; Salinity ; Phosphate ; Cyanophyta ; Annelida ; Mollusca
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25397 | 18721 | 2018-09-20 11:42:43 | 25397 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Sturgeon stocks are drastically decreasing in Caspian Sea during recent decades. This project has been proposed to collect the excess sperm of Caspian Sea Southern part sturgeon breeders, to cryopreserve and hold them for further use. During two spawning seasons (2006-2007), sperm quality and quantity analysis were conducted on 34 different male sturgeons of which 12 Persian sturgeon, 4 stellate sturgeon, 1 beluga and 1 ship sturgeon with better quality were chosen to cryopreserve. After dilution of fresh sperm with sturgeon sperm diluent (115 mM Tris, 23.4 mM sucrose and 15% DMSO) at the ratio of 1:1, the 0.5 ml straws were filled using special filling and sealing apparatus and frozen with a multi step freezing rate. To access the further quality and fertilization test, samples were thawed at 40 °C for 20 seconds. During the project performance, 1010 ml of Persian sturgeon sperm , 110 ml of ship sturgeon sperm , 90 ml of stellate and 80 ml of beluga sperm were cryopreserved. The reduction of frozen-thawed sperm motility in comparison with fresh samples showed 10-52.3 % decrease, but there was not any significant differences between samples after 7 months preservation in liquid Nitrogen (P 〈 0.005). River caught Persian sturgeon samples showed higher sperm density in comparison with nonriverine breeders (P 〈 0.05). Data showed that stellate sturgeon sperm showed better resistance to freezing condition in comparison with Persian sturgeon spermatozoa. Fertilization tests (4 hours after fertilization) showed 27.4% and 58.2% fertilization rate in frozen-thawed stellate and Persian sturgeon spermatozoa, respectively.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Spermatozoa ; Cryopreservation ; Percentage of motility ; Sperm bank ; Sturgeon ; Brood stocks ; Spawning ; Fertilization ; Samples ; Density
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25405 | 18721 | 2018-09-20 12:38:52 | 25405 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The project of the Study on fishing and resource management of fisheries within Southern Caspian Sea was conducted on the base of an agreement made between fisheries organization (Shilat) and the Iranian fishery research organization (IFRO) signed in 2010. The study has several phases. The final report of the fishery on fishing and resource management of fisheries within Southern Caspian Sea (Gilan, Mazandaran & Golestan provinces) is consisted of several sections such as: 1- The Survey status of biology and catch of Kilka fishes , Bony fishes and Estrogen fishes and also the status of aquatic resource conservation in the Southern part of Caspian Sea 2- Fishing analysis in the Southern part of Caspian Sea 3- Fisheries development Strategies, policies and plans in the Southern part of Caspian Sea In this survey and library-based study, internal and external environmental factors of shilat activities are considered. Using SWOT analysis method, current situation of fisheries is investigated. In this regard, all fisheries activities were divided into four groups, then, the strengths, weaknesses, opportunities and treats of internal and external environment will be analyzed. A considerable number of tables and matrixes were prepared and policies and strategies as seen of final report were carried out.
    Keywords: Fisheries ; Iran ; Caspian Sea ; Gilan province ; Mazandaran Province ; Golestan Province ; Resource management ; IFRO ; Shilat ; Fishing ; Kilka ; Fisheries ; Survey ; Biology ; Bony fishes ; Estrogen ; Environmental factors
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25412 | 18721 | 2018-09-23 15:56:29 | 25412 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Using SWOT analysis with traditional matrix and quantitative matrix methods, the strategies to benefit from strengths and opportunities to vanishing weaknesses and decrease of threats for strategic management of aquaculture in Iranian Northern provinces was surveyed. Utilizing researches held in fisheries research centers and existence of aquaculture experts in vicinity, activation of veterinary organization in production and post production, and entering new brood stocks with desirable growth are the main strategies for developing rainbow trout and Chinese carps culture in Iranian Northern provinces. In sturgeon fishes culture, independence of farms from fingerlings produced by governmental complex and diversification the cultured fishes using new species and hybrids can develop this part of aquaculture. Avoiding illegal fishing in sea and rivers, preserving the population of Caspian Sea fishes, entering new fish species which their resources are decreased extremely to breeding and fingerling production cycle, and using pike perch and bream while they migrate to Anzali Lagoon from Caspian Sea, instead of freshwater brood stocks will cause the output of sea enhancement and sea ranching activities to increase, and fisheries return coefficient of these fishes will rise. Using quantitative matrix and scoring method, it was defined that aquaculture in Iranian Northern provinces are extremely approved and acceptable and the score of strengths is more than weaknesses and opportunities more than threats. By using strategic management, these activities can be more developed.
    Keywords: Management ; Iran ; Caspian Sea ; Anzali Lagoon ; SWOT ; Strategic management ; Aquaculture ; Sea enhancement ; Sea ranching ; Traditional matrix ; Quantitative matrix ; Fisheries ; Survey ; Culture ; Species ; Illegal fishing
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25424 | 18721 | 2018-09-23 16:21:43 | 25424 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In order to diversify the production of carp (Cyprinid) in Iran, in 2010 September, 15,000 pieces of fry from three species of indian major carp were arrived to Iran. After subtraction of the dead fishes, finally 10,500 fry were introduced to 700 m2 ponds with a density 3,500 pieces, which includes 46. 7 % Catla, (Catla catla), 35 % Rohu (Labeo rohita) and 18. 3% Mrigal Mrigal (Cirrhinus mrigala). Primary average weight and length of these fishes were 0.3±0.0 g and 23.6±0.75 mm for Rohu, 0.1±0.0 g and 33.26±0.35 for catla and 0.16±0.01 g and 34.8±0.96 mm in Mrigal respectively. Fish were reared in the Khuzestan province's climate with at least 7°C and a maximum temperature of 35°C. The culture period was 6 month. Once every 45 days by using signebeach net samples were taken from ponds and biometry tests were done. The maximum net production was observed at 50% situated treatment, with average weight 723.3 kg per 1700 m^2 ponds (4254.9 kg ha). There is no significant differences between experimental treatments with each other and with control (P 〈0. 05). Mean final weight of Rohu in 3 experimental treatments were 1136, 1129, 1054 g and in Catla and Mrigal were 726,902, 839 g and 773, 788, 894. There was not significant diference between them (P〈0.05). Survival rate in Rohu, Catla and Mrigal was 92%, 97% and 86% respectively. The maximum specific growth rate (SGR) of fish related to Catla (1.92) and the minimum SGR of fish was observed in Mrigal (1.45). In accordance with the results of this study, the Indian carp fishes can able to adapt, grow and reach to market weight in the Khuzestan province's weather conditions.
    Keywords: Aquaculture ; Iran ; Khuzestan province ; Indian carp ; SGR ; Labeo Rohita ; Cirhinus merigala ; Catla catla ; Chainess crap ; Cyprinid ; Species ; Temperature ; Survival rate
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25403 | 18721 | 2018-09-20 12:22:36 | 25403 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Sediments samples were collected using Veen Grab Sampler (0.1 square meter) at 8 transects namely Astara, Anzali, Sefidroud, Tonekabon, Noshahr, Babolsar, Amirabad, Torkman in the southern of Caspian Sea. Five stations were selected at 5, 10, 20, 50 and 100 meter depths in each transect. Sediments were sampled triplicate at each station. Samples also were collected during four seasons (spring (May), summer (July), fall (November) and winter (January)) in 2009. Results of this study showed that species composition of Macrobenthos consisted of 32 species which belonged to 7 families of Polychaeta, Crustacea and Bivalvia at studied area. In addition, Oligochaeta identified in “Class”, Chironomidae considered in Insecta categories “Family” and Streblospio spp. (Polychaeta) was recognized in “Genus”. Gammaridae and Pseudocumidae of Crustacea with 12 and 10 species had the highest species diversity compared to other groups, respectively. Polychaeta was consisted 75.5 percent of total abundance of macrobenthos which the major abundance (equal 62.4% of total abundance) were belong to Streblospio spp. from Spionidae family, while its biomass was equals 5.11% of total macrobenthos. In contrast, Cerastoderma lamarcki species from Bivalvia Class with only 1.7% of total abundance of macrobenthos allocated 69 percent of total biomass. In the southern of Caspian Sea, average total abundance was significantly less at 4 western transects (Astara, Anzali, Sefidroud, Tonekabon) compared to 4 eastern transects (Noshahr, Babolsar, Amirabad, Torkman) (p〈0.05). The highest average abundance of macrobenthos (10655±1246SE ind/m^2) was observed at transect of Torkman, and lowest value (4032 ± 686SE ind/m^2) was recorded at transect of Sefidroud (p〈 0.05). Generally, minimum species diversity were obtained at 20 m depth in all transects and the maximum value was observed at 5 m depth in most of transects (p〈0.05). In contrary, maximum average abundance of Macrobenthoses was at 20 m depth in transects of Anzali, Sefidroud, Tonekabon, Nowshahr and Amirabad compared to other depths. Macrobenthoses abundance average in 5 m depth (except Astara and Torkman) was less than other depths in 6 transect (p 〈 0.05). Total average abundance and biomass of macrobenthos was 5976±583SE ind/m^2 and 43.675 ± 11.402SE gr/ m^2, respectively. Maximum and minimum of abundance of macrobenthos were observed in summer (7714±778 ind/m^2) and winter (4071 ± 340 ind/m^2), respectively. Maximum and minimum of biomass of macrobenthos were obtained in fall (50.271±13.258SE gr/ m^2) and in summer (35.123 ± 8.903SE gr/ m^2), respectively (p〈 0.05). Percent of total organic matter (TOM) were low in 5 and 10 m depths and increased toward offshore depths. TOM percent was 2.06±0.11SE at 10 m depth and increased to 4.62 ±0.17SE in 100 m depth. Percent of silt and clay (grains size less than 63 micron) had positive significantly correlation with percent of TOM (p〈0.01).While they had negative significantly correlation with percent of sand (grains size between 63 and 1000 micron) (p〈0.01). Percent of silt and clay like organic matter, had ascending trend toward to depth increased and varied from 44.4 ± 4.06SE percent in 5 m depth to 96.5 ± 0.59SE percent in 100 m depth. In contrast, percent of sand decreased toward depth and varied from 54.5 ± 4.13SE percent in 5 m depth to 2.8 ± 0.53SE percent in 100 m depth. Result of current study showed that total abundance of macrobenthoses had positive significantly correlation with TOM percent (p〈0.01) and silt/clay percent (p〈0.05). Abundance of Oligochaeta had positive significantly correlation (p〈 0.01) with TOM and silt/clay percent. Two groups of Polychaeta, Gammaridae and Cerastoderma lamarcki had negative significantly correlation with TOM and silt/clay percent (p〈 0.01), and every four aforementioned groups had positive significantly correlation with sand percent (p〈 0.01). Overall, different correlation between abundance of various macrobenthos groups and TOM percent and type of grain size of sediment could be related to fluctuation of abundance of various macrobenthos groups at difference transects and depths. On the other hand, in study area were occurred simultaneously some phenomena such as increased abundance of Oligochaeta and Polychaeta, dominance of Streblospio Genuse (Polychaeta group), and decreases abundance of Bivalvia and appearance of Menemiopsis leidyi which need to study more and monitoring of this area.
    Keywords: Ecology ; Iran ; Caspian Sea ; Astara ; Anzali ; Tonekabon ; Noshahr ; Babolsar ; Sefidroud River ; Macrobenthos ; Diversity ; Distribution ; Abundance ; Biomass ; Survey ; Fauna ; Sediments ; Samples ; Streblospio spp. ; Cerastoderma lamarcki ; Menemiopsis leidyi
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25413 | 18721 | 2018-09-23 16:29:08 | 25413 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This survey was carried out by R/V Gilan with a conical plankton net of 100 micron mesh by vertical hauls at 8 transect in the southern Caspian sea. Transects were located at Astara, Anzali, Sefid roud, Tonekabon, Noshahr, Babolsar, Amirabad and Torkman in depths of 5, 10,20,50and 100m. Samples were taken in 4 seasons (in each season 64 samples) in 2009. In this study 23 species formed the zooplankton community including 4 species of Copepoda, 8 species of Rotatoria, 2 species of Protozoa and 8 species of Cladocera. The meroplankton blonged to 2 species of Balanus sp and Lamellibranchiata larvae. The annual results revealed that the maximum abundance of Copepoda were 3612±4839 ind/m^3 and 35/75±42/81 mg/m^3 in summer and decreased gradually since autumn and reached to minimum value in winter. The maximum abundance of Cladocera was 300± 383 ind/m^3 in spring and reached to less than 4 ind/m^3 in summer and autumn. The Rotatoria had high population (3775± 8632 ind/m^3) in winter which consisted the zooplankton population with Copepoda. The Protozoa had no efficient role in zooplankton population of Caspian sea during the year. The zooplankton population was affected by Meroplankton and Lamellibranchiata larvae in spring and winter. The Copepoda contributed in zooplankton population only in summer and autumn. The maximum zooplankton abundance and biomass were in 5m depth which included 10209±8352 ind/m^3 and 43.56±26.08mg/m^3 in spring, 8545±7127 ind/m^3 and 73.59±56.65 mg/m3 in summer (10 m depth), 8427±6711 ind/m^3 and 43.15± 29.59 mg/m3 in autumn and 24426±26975 ind/m^3 and 334.25±392.21 mg/. m3 in winter which decresed from surface to depth. The maximum abundance and biomass of zooplankton were 5119±6018 ind/m^3 in east and 28.59±24.79 mg/m^3 in central area in spring, 6628±7117 ind/m^3 in west area and 50.73±59.52 mg/m^3 in central area in summer, 5248±5489 ind/m^3 and 31/16 ±31/22 mg/ m^3 in west area in autumn and 11588±16191 ind/m^3 and 131.02±193.61 mg/ m^3 in west area in winter. The annual statistical analysis reveals that there is significant difference between Copepoda and Cirripedia population among seasons, sampling stations, depth and sampling layer (Kruskalwallis test, p〈0.05) and the total zooplankton populatin had no significant difference only between seasons (Kruskal-wallis test, p〉0.05). The Cladocera, Lamellibranchiata larvae and the Rotatoria had significant difference between seasons only (Kruskal-wallis test, p〈0.01).
    Keywords: Ecology ; Iran ; Caspian Sea ; Astara ; Anzali ; Tonekabon ; Noshahr ; Babolsar ; Zooplankton ; Copepoda ; Meroplankton ; Survey ; Plankton ; Samples ; Species ; Cladocera ; Meroplankton ; Balanus sp. ; Lamellibranchiata ; Larvae
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  • 76
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25431 | 18721 | 2018-09-23 16:47:43 | 25431 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Comb jelly Mnemiopsis leidyi investigation was conducted in the southern region of the Iranian coast of the Caspian Sea during the 2010 in the seasons of spring, summer, autumn and winter at 8 transect with depths of 5, 10, 20, 50 and 100m. Sampling was done at 0–5, 0-10, 0-20, 20-50 and 50–1000 m using a 500 µ and 50 cm mouth opening area. During 2010, a total of 757 samples of comb jelly were collected by the size length of smaller than 5 mm to a maximum of 35 mm. Mean annually Mnemiopsis abundance and biomass was 11±2 ind.m3 and 0.6±0.1 g.m^3 . Maximum abundance was recorded in autumn with 25.7±4.8 ind.m3 and maximum biomass was observed in summer of 1.2±0.1 g.m^3. Minimum abundance was found in spring with 2.3±0.4 ind.m3 and minimum biomass was in winter of 0.1±0.3 g.m^3. Statistical comparison showed that the density of comb jelly were not significantly different in spring and summer (P〉0.05, Tukey test), and winter and summer with the same density, while having the highest density of autumn compared to other seasons which showed a significant difference (P〈0.01, Tukey test). Comb jelly biomass showed summer and spring with higher than winter and autumn, compared to the statistical comparison showed no significant difference in biomass between seasons of summer- spring and fall – winter (P〉0.05). Fluctuations of comb jelly abundance and biomass in different seasons showed that the water level of 20 m had a higher value than other layers. In the spring, more than 98 percent of the comb jelly was recorded in the upper layer (less than 20 m), and only 2 percent of the animals living in layers of 20-50 m. In the summer and autumn, about 93 percent of Mnemiopsis was found in the layers above 20 m and 7 percent of those in the lower layers (maximum 50 meters). In the winter, more comb jelly were observed in layers from 20 to 50 m (about 14 percent) and about 87 percent of them were collected in layers less than 20 meters. As a whole, the total maximum annual average abundance was recorded in the autumn of layer 0-5 m with 94.0± 33.8 ind.m3 and the highest average biomass in the 0-5 m layer of summer with 3.1±1.0 g.m^3 . Frequency of group length (size) of the population M. leidyi showed that comb jellies of less than 15 mm (prior to adult) consisted of 85.8%, in which at less than 20 m, group length (size) of 15 mm included of 82-92% and at the depth of more than 50 m, these group was 8-18%. Also comb jelly adults (greater than 16 mm) in the surface layers (less than 20 m) were included of approximately 73 to 100% and below 20 m, 0-27 % of the population. Data of the 8 transect showed that the highest density and biomass of Mnemiopsis were recorded in Torkaman with 16.4± 6.5 ind.m^3 , and 0.8±0.2 g.m^3 , respectively. Statistical comparison of the mean abundance and biomass of comb jelly showed that there were a significant differences between Turkmen and Amirabad transect compared to other transects (P〈0.05). Regional distribution of M. leidyi in the southern Caspian Sea showed aggressive area in terms of density and biomass are areas with significant differences (P〈0.05). Comb jelly population showed that both West and East in terms of abundance and biomass were similar but different with the central region. Eastern and western regions compared to the central region have the higher abundance. Based on the study looks at the rise and persistence parameters of comb jelly, it seems two main factors such as water temperature to the desired value, especially in summer (22-30°C), and food availability (zooplankton) have more roles in the southern of the Caspian Sea. After the invasion of M. leidyi into the Caspian Sea, not only the abundance and biomass of zooplankton decreased but also the species diversity decreased from 36 to 15 species. Hence, it seems the decline in the South Caspian comb jelly was due to decreased of fecundity for lack of zooplankton feeding resources.
    Keywords: Aquaculture ; Ecology ; Iran ; Caspian Sea ; S Caspian Sea ; Mnemiopsis leidyi ; Abundance ; Biomass ; Distribution
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  • 77
    Publication Date: 2021-07-16
    Description: In this study, first, the influence of three precooking methods (steaming, oven-baking and microwave-cooking) on the contents of mineral elements-copper, zinc, iron, calcium and sodium-of silver carp (Hypophthalmichthys molitrix) was evaluated. Determination results of evaluated elements in raw fish and cooked samples were in range 3.05 to 4.19 for copper, 71.45 to 82.85 for zinc, 32.18 to 40.70 for iron, 425.6 to 529.46 and 315.5 to 534.76 for calcium. Results showed different precooking treatments had no significant effects on the amounts of mineral elements such as copper, zinc, calcium and sodium. The Iron content in the samples subjected to microwave cooking increased. With doing multivariate analysis, on comparing the raw and precooked fillets, steam cooking found to be the best precooking method on retain mineral elements. After choosing the best precooking method, for evaluating the influence of different filling media including sunflower oil, soybean oil, olive oil and brine, on the quality of canned silver carp, amounts of mineral elements(copper, zinc, iron, calcium and sodium) and microbial indices(total counts, thermopile count and clostridium) and sensory indices (color, smell, taste and texture) was surveyed. Assessments of microbial indices showed no microbial activity in canned products. After canning amounts of sodium increased in all the treatments. Except for iron, using soybean oil had no significant effect on the contents of other elements. The highest levels of iron and copper were observed in olive oil canned samples sterilized at 130°C. Sensory evaluating showed, kind of filling media had no significant effects on sensory indices such as taste, smell and color of canned samples. The texture of soybean oil canned samples and the quality defects of olive oil and brine canned samples had better condition than other treatments. In the last step, canned silver carp were proceed under three different temperatures (115°C, 120°C and 130°C) with equal lethality value (Fo=7min), then sensory indices and amount of mineral elements were compared. Results showed, the contents of iron, copper, sodium and calcium were changed in soybean oil canned sample. The amounts of copper and sodium in sunflower oil canned sample subjected to different heating regimes showed significant variation. The highest amount of copper was observed in the canned samples subjected to 130°C heating regime. Results showed contents of iron and copper of olive oil canned sample subjected to 120°C and 130°C heating regimes were higher than sample subjected to 115°C heating regime, while the zinc and calcium contents had no variation. In brine canned samples the highest amounts of copper and iron was obtained after sterilization in 120°C. Results of sensory evaluation showed different heating regimes had no significant effects on the indices of taste, smell, and color of products. Doing 130°C heating regime in brine canned samples led to obtain the better tenacity of texture. While this heating regime caused to increase the quality defects of soybean oil canned samples as a result of existence of hard parts of bone.
    Keywords: Chemistry ; Fisheries ; Iran ; Precooking ; Filling media ; Heating regime ; Sterilization ; Silver carp ; Mineral elements ; Hypopthalmichthys molitrix ; Samples ; Sunflower oil ; Soybean oil ; Silver Carp
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25438 | 18721 | 2018-09-23 16:51:04 | 25438 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Caspian trout is valuable, Commercial, anadromous and endemic fish species in the Caspian Sea so that, researchers attention have been focused on this species. At the moment, only pay attention to stocks rehabilitation that fisheries organization releases more than 300,000 fingerlings to the rivers ends to the Caspian Sea. Importance of this study consist (1) introducing a new species to the aquaculture system and (2) provide breeders in this research. we used from 2 treatments that consist with density of 10 kg/m^3 and 20 kg/m3 with 3 replication. Results showed, culture of Caspian trout is suitable with density 10 kg/m^3, so that weight gain, SGR and Daily growth mean are better than treatment 2(15-20 kg/m^3 )
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Salmo trutta caspius ; Growth factors ; Feasibility ; Culture ; Brackish water
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25440 | 18721 | 2018-09-23 16:55:22 | 25440 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was conducted to determine 16 Polyaromatic Hydrocarbons (16 PAHs) concentrations in water (during four seasons) and surface sediments (during summer and winter) at eight transects (Astara, Anzali, Sefidroud, Tonekabon, Nowshahr, Babolsar, Amirabad and Turkman) in the southern of Caspian Sea in 20102011. 94 samples of water and 45 samples of surface sediments were collected at 10, 50 and 100 meters depths. In addition, 28 samples of fish (Cyprinus Carpio) were collected during winter and spring. All samples were prepared by Soxhlet and extracting processes and then determined using High Performance Liquid Chromatography (HPLC) instrument. Results of current study showed that mean concentration and standard error (±SE) of 16PAHs water were observed 232 (±77), 1268 (±808), 538 (±190) and 151 (±53) µg/l in spring, summer, fall and winter, respectively. In addition, annual mean contents and standard error (±SE) of 16PAHs water were registered 24.10 (±8.12) µg/l. The Hazard Quotation (HQs) were calculated more than unit and belong to Benzo(a)pyrene and Dibenzo(a,h)anthracene compounds. Mean concentration and standard error (±SE) of 16PAHs sediment were observed 0.77 (±0.23) and 1.21 (±0.64) µg/g.dw in summer and winter, respectively. Also, the annual mean contents and standard error (±SE) of surface sediments were observed 0.93 (±0.33) µg/g.dw. The Hazard Quotation (HQs) were calculated more than unit and belong to Fluoranthene, Benzo(a)anthracene, Chryseneand Benzo(a)pyrene. The annual mean contents and standard error (±SE) of edible tissue of Cyprinus Carpio mussels were observed 2.21 (±0.42) µg/g.dw. Annually, pattern of 16PAH compounds were obtained 10, 70, 12 and 8% for 3,4,5, and 6 rings, respectively. Four ring compounds had high content and percentage and 2 ring was not observed in all samples of water. Also, pattern of 16PAH in surface sediments were obtained 31, 56 and 14% for 3, 4, and 5 rings, respectively. Four ring compounds had high content and percentage and 2 and 6 rings were not observed in all samples of surface sediments during two seasons. Result of Diagnostic Ratios analysis (DRs) of sediments showed that the source of oil compounds were petrogenic and pyrogenic in summer and winter, respectively. Based on DRs in water and sediments were observed oil compounds were either petrogenic or pyrogenic with different percentage in the southern Caspian Sea. In conclusion, the results revealed that in the some transects the 16PAHs concentrations of water were above the threshold levels and more petrogenic (85%) sources which represented polluted condition in this area. 16PAHs concentrations of sediments were below the threshold levels and conditions for this region were showed unpolluted. Also, the Caspian Sea sediments were classified in Class 2 (Fair). According to results of the evaluation’s risks to human health associated with consumption of the mussels containing 16PAHs suggest that there is risk for humans.
    Keywords: Pollution ; Iran ; Caspian Sea ; Astara ; Anzali ; Tonekabon ; Nowshahr ; Babolsar ; Sefidroud River ; Oil pollutions ; Water ; Sediments ; Fish ; Cyprinus carpio ; Samples ; Polyaromatic ; Hydrocarbons
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  • 80
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25452 | 18721 | 2018-09-26 09:35:53 | 25452 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: At the present study, the water quality parameters monthly, environmental pollutants (pesticides, heavy metals and surfactants) seasonally, macrobenthos and bacteria (monthly) were done 2009 t0 2010 that located in Mangol Dam with longitude and latitude 52° 22' 49" N and 43° 15' 43" E, respectively. All water samples were collected from under-groundwater, surface water and wastewater in 12 stations and the results are as follow: The mean concentrations of DO, BOD_5, COD, HCO^3-, CO_3^2-, TA, Ca^2+, Mg^2+, TH, TSS, TDS, Cl^-, PO4^3-, NH^4+, NO^2- and NO^3- were determined about 11.86±0.49, 5.95±0.11, 4.96±0.59, 28.1±6.1, 14.34±1.64, 36.30±2.33, 64.66±2.34, 28.84±1.52, 271.62±8.41, 0.37±0.03, 0.45±0.02, 28.02±1.96, 0.06±0.01, 0.010±0.001, 0.01±0.001 and 0.96±0.03 mg/l, respectively and also temperature, pH and EC were 12.7±0.61°C, 8.00±0.02 unit, 0.57±0.02 ms/cm in all samples. The surface water quality of Haraz River and its tributaries were suitable for usual applications such as aquatic matters, but based on BOD_5 need to more attention for human usages. The water quality index (WQI) of surface water was classified in group II at all stations that shows serious water quality changes due to domestic and agriculture sewage. The groundwater was affected by different matters that caused change of water quality parameters. The wastewater was classified from good to moderate based on BOD5. The quality of wastewater was declined at down-stream with decreasing DO and pH and increasing BOD5 pollutants. The mean concentrations of aldrin and endrin were about 0.66 ± 0.59 and 0.71 ± 1.07 µg/l, respectively. The maximum seasonal concentration of organochlorin pesticides (OCPs) were detected in fall, winter and spring for endosulfan sulfate, γ-BHC, heptachlor, endrin aldehyde compounds about 2.85, 0.34, 0.14 and 0.14µg/l, respectively. The maximum seasonal percentage range of OCPs in fall, winter and spring for endosulfan sulfate, DDD and β-BHC were 42, 25 and 25 %, respectively. The maximum seasonal concentrations during fall, winter and spring were detected in Sorkhroud and Polechelave stations for only Fe about 1.48, 3.3 and 8.22 µg/l, respectively. Also for Hg in spring was determined about 17.2 mg/l. The mean concentration of Zn, Fe and Hg in water sampled were 0.23 ± 1.01 and 1.21 ± 2.00 µg/l, respectively and 4.65± 6.38 mg/l. The maximum concentration of linear alkyl benzene sulfonate (LAS) in summer was 971µg/l. The mean concentration of surfactant during fall, winter, spring and summer was 418.62 ± 52.16 µg/l. The maximum seasonal percentage ranges of surfactant during fall, winter, spring and summer in all stations of sampling were 76, 76, 100 and 100 %, respectively. Tottaly 15 families of macrobenthos were identified in Haraz River. The maximum mean of abundance and biomass in Sorkhroud and Nour-roud stations were 1177±256 ind/m^2 and 3609±935mg/m^2, respectively. During Seasons, the maximum mean abundance and biomass were detected in fall and winter with 1185±444 ind/m^2 and 3891±849 mg/m^2, respectively. Water quality were classified by Hilsenhoff Biotic Index such as Sorkhrood station as "Very Poor", Isar town station as "Fairly Poor" and Karesang and Nor-roud stations as "good" qualification. The maximum of total coli forms and fecal coli forms in surface water were observed in Sorkhrood about 4.9 and 2.3CFU/100ml, respectively. The maximum of total coli forms and fecal coli forms in groundwater were observed in Halomsar and Absak stations about 4 and 1.7CFU/100ml, respectively. Maximum of total coli forms and fecal coli forms in wastewater were observed in Nor-roud station about 3.1 and 1.6CFU/100ml, respectively. The Ovary of nematode in wastewater were observed in Nor-roud and Isar Town stations about 30 through 124, respectively.
    Keywords: Ecology ; Iran ; Haraz River ; Mazandaran province ; Mangol Dam basin ; Physico-chemical characteristics ; Surface water ; Ground Water ; Wastewater ; Pesticides ; Heavy Metals ; Surfactants ; Macrobenthos ; Coliforms ; Nematodes ; Environmental pollutants ; Bacterial ; Temperature ; pH ; Biomass
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25468 | 18721 | 2018-10-02 10:04:52 | 25468 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This survey was carried out by R/V Guilan ship with a conical plankton net of 100 micron mesh by vertical hauls at different stations and depths of 5, 10, 20, 50 and 100m in 4 seasons of 2010. In this study, 16 species formed the zooplankton community including 4 species of Copepoda, 4 species of Rotatoria, 2 species of Protozoa and 4 species of Cladocera from Holoplanktons and 2 species of Balanus sp and Lamellibranchiata larvae from Meroplanktons. The annual results revealed that the mean abundance of zooplankton were 5477±5815 ind/m^3 and 5131±7908 ind/m^3 in spring and winter respectively which were more than other seasons but the maximum biomass (64/58±124/61mg/m^3 ) was in winter that was affected by Rotatoria. The maximum mean abundance and biomass of Copepoda were in summer that were 2830±2342 ind/m^3 and 22/52±21/78 mg/m^3 ,respectively and the abundance decreased gradually since autumn and reached the least in winter but increased since spring. The mean maximum abundance of Cladocera (115 ±142 ind/m^3) was in spring but it decreased gradually, so it reached to less than 3 ind/m^3 in summer. The Protozoa constituted the least community and biomass of zooplankton in southern area of Caspian Sea. The zooplankton community also was affected by Meroplanktons including Cirripedia and Lamellibranchiata Larvae in spring and the Rotatoria contributed highly in zooplankton community in winter with the mean abundance and biomass of 2604±5876 ind/m^3 and 50/71±115/33 mg/m^3 respectively.Other than Copepoda, other planktonic groups contributed lowly in zooplankton community. It was observed the reduction trend in abundance from surface depths to deeper depths in whole of the year. There were 75% of zooplankton community in stations with 5 to 20m depth and 25% of community in stations with 50 to 100m depth in spring, and also 74% in stations with 5 to 20m depth and 26% in stations with 50 to 100m depth in summer, 73% in stations with 5 to 20m depth and 27% in stations with 50 to 100m depth in autumn and 85% in stations with 5 to 20m depth and 15% in stations with 50 to 100m depth in winter. In analysis of different area of southern basin of Caspian sea the maximum abundance was observed in west in spring, summer and winter which were 7514±8115 ind/m^3, 3909±2609 ind/m^3 and 8129±11587 ind/m^3, respectively. There was 2283±2134 ind/m^3 in center area in autumn. The annual statistical analysis revealed that, there was significant difference in total zooplankton community between sampling stations, depths and layers in whole of the year.
    Keywords: Ecology ; Iran ; Caspian Sea ; Zooplankton ; Meroplankton ; Species ; Abundance
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  • 82
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25165 | 18721 | 2018-09-03 17:03:11 | 25165 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The present project in related to survey of factors and hydrology and hydrochemical features (water temperature, dissolve oxygen saturation, pH, clearance, salinity, nitrogen, phosphorus and silicon) hydrobiology (zooplankton, phytoplankton, macrobenthos) and survey of bio environment pollution (oil, heavy metal, detergent) executed in lower 10m in different water larger in southern Caspian Sea in 2002-2003. For sampling 8 lines number were vertical on coast that selected from Astra in west to Gomishan in east in southern Caspian Sea basin. The result indicated the average physical factors such as pH were 8.11 and salinity12.12 ppt ,and disolve oxygen6.7 mg/l. Average chemical factors such as NO_2 , NO_3 and NH_4 were 1.2 µg/l, 25.7 µg/l, 13 µg/l respectively.Total nitogen and organic nitrogen and inorganic nitrogen were 690.2 µg/l , 667.6 µg/l , 41.6 µg/l. Average silicat were recrded 266.35 µg/l . Total Phosphorus was observed 37.35 µg/l and average of organic Phosphorus concentration was and 20.25 µg/l .Average of Total organic matter (T.O.M) was 4.98% maximum amount were observed in Lisar and minimum in Nooshahr . Concentration of heavy metal during sampling were respectively ,Fe〉Mn〉Zn〉Cr〉Ph〉Co〉Cd〉Cu. Maximum concentration of Fe were determined in winter in Nooshahr and Babolsar respectively 13/3 µg/l 17/1 µg/l. In many stations and different Season, the amount of heavy meta were lower standard of in marine water. The concentration of oil hydrocarborate (PAHs) in autumn was 0/13 ppb and in winter 0/12 ppb. The amount of (PAHs) in Southern Caspian Sea were Lower than other parts of Caspian Sea. The average of detergent concentration (LAS) was 0/036 µg/l that was two fold higher than determined in 2001. Total 107 species of phytoplankton belong to 5 phylum were identified. The numbers of species of phytoplankton groups were respectively, chrysophyta (42 species), cyanophyta (17 species), pyruphyta (17 species), chlorophyta (21 species) and euglenophyta (9 species). The maximum diversity of phytoplankton observed in summer and minimum in autumn. High diversity of chrysophta and cyanophyta observed in summer and phyrophyta and chrlophyta in spring. The composition of phytoplanhkton groups were respectively, chrysophyta (70%), phyrophyta (9%) and chlorophyta (7%) and euglonophyta (1%). Maximum density of phytoplankton was observed in autumn and minimum in winter. Total 19 species of Zooplankton were identified. Maximum diversity was observed in summer and minimum in winter. Zooplankton changes during sampling, showed amount of density of zooplankton in 5m were more than 10 m depths. Total (17 species macrobenthos were identified. The composition of macrobenthos groups were respectively , Annalida (92/7% ) , Bivalvia (2/7%) gumarida (108%) cumacea ( 1/5%) , Balanidae 103% . max . density were observed in Astara and min . in Sefied roud Average of density were 1218 0/851 ind /m^2 and biomass 14 15 g/m^2 High density were recorded in autumn and low density in winter . Correlation of phytoplankton and zooplankton with physicochemical parameter and also relation between total organic matter and sediment grain size were calculated.Ecological indicies (simpson diversity evenns diversity and shanoon-wiever diversity) were calculated for macrobenthos. Data were shown impact of cetenephora (Mnenemiopsis leidyi) on zooplankton and phytoplankton and macrobenthos density.
    Keywords: Ecology ; Pollution ; Iran ; Caspian Sea ; S Caspian Sea ; Phytoplankton ; Zooplankton ; Macrobenthos ; Heavy metals ; Pollution ; Oil pollution ; Detergent ; Hydrology ; Hydrochemistry ; Species
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  • 83
    Publication Date: 2021-07-16
    Description: The Sampling of macrobenthic communities in southeastern Caspian Sea, Iranian coast, were investigated seasonally from April to March 2012. Sampling was carried out at 8 transect as named Astara, Badar Anzali, Sefied rood, Tonekaboon, Nooshahr, Babolsar, Amirabad, Gomishan.In each transect were choice 8 stations at 5m, 10m, 20m, 50m, 100m depths respectively. Samples were taken in triplet with a 0.1m^² van Veen grab.Totally 28 macrofaunal species belonging to 10 families were identified as gamaridae (9species), corophidae (3species),psudocumidae(8 species), amphartidae (2species), balanidae, chironomidae, cardidae scorbicolaridae, nereidae, sponoidae, each one species. Polychaetes were dominated, representing 72.5% of the whole fauna while while (equal 62.4% of total abundance and 5/1%biomass) were belong to Streblospio spp. from Spionidae family. Cerastoderma lamarcki belong to bivavia consist of 2/3% total macobethos abundance and 73% biomass. Total abundance of macrobenthos of middle area were higher than east and west area in southern Caspian Sea. Average benthic population density (no/m^2) with (SE) was 4581(444 ). The benthic biomass (mg/m^2) with an overall mean of 12. 125(02). Spatial and temporal variations of benthos. In the present study showed, maximum abundance Macrobenthos 17855(5543) no/m^2) were recorded in 20 meter depths at Nooshahr trancect , while minimum 841(109 )in 10 meter depths at Amirabad trancect p〈0.05. Maximum biomass 135.10(126) g/m^2 were obtained at 10m depths in Astara minimum 0.51(0.4) ) g/m^2 at 5m depths in Nooshahr transect . maximum were obtained in winter and minimum in summer Number of species varied 3 to 18 , Generally, minimum species diversity were obtained at 20 m depth in Tonekabon transects and the maximum value was observed at 100 m depth of sefeidrood variations physicochemical factors were between salinity 12.5to12.9ppt ,temperature 8-19˚c and PH 8.1 dissolve oxygen 69-101mg/li. Total secondary production calculated, according ,104/996g AFDW/m^2/yr.Maximum were recorded in 10meter depth (30/381AFDW/m^2/yr) and minimum at 50 meter depth(11/007g AFDW/m^2/yr) respectively. Average total organic matter were 3/18 percent in sample periods. Percent of total organic matter (TOM) were low in 5 and 10 m depths and increased toward offshore depths. TOM percent was 1/67(0.11 ) at 5 m depth and increased to 4/38(0.13 )in 100 m depth. Average total silt &clay were 68/63(2.97) percent in sample periods. Positive significant relation was between silt &clay and total organic matter. Macrobenthos community reduce in high silt clay and high sandy contents. Due to increase of streptolosbio.spp, monitoring program for understanding effect of this organism on ecosystem and on fish feeding is necessary.
    Keywords: Ecology ; Iran ; Caspian Sea ; Astara ; Badar Anzali ; Tonekabon ; Macrobenthos ; Diversity ; Distribution ; Abundance ; Biomass ; Secondary production ; Species ; Gamaridae ; Corophidae ; Psudocumidae ; Amphartidae ; Balanidae ; Chironomidae ; Cardidae ; Scorbicolaridae ; Nereidae ; Sponoidae ; Salinity ; Temperature ; Streptolosbio.spp
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  • 84
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25514 | 18721 | 2018-10-05 15:36:40 | 25514 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was conducted to determine growth parameters, catch and fishing effort trends, stock assessment and Acceptable Biological Catch (ABC) of bony fishes in the Iranian coastal waters of Caspian Sea in the years 2010-2011 and 2011-2012. According to the result, the numbers of beach seines were 131 and 128 and their fishing efforts were observed 50184 and 42255 beach seining during 2010-11 and 2011-12, respectively. The catch per unit of effort CPUE) was calculated 182.9 and 205.6 kg/haul during two sampling periods, respectively. The total catches (including illegal fishing) were also obtained 16601.5 mt and 17034.1 mt during 2010-11 and 2011-12, respectively. The highest proportion of catch was belonged to kutum and golden grey mullet (86% and 88%, respectively) in two fishing seasons aforementioned above. Growth parameters of kutum were estimated as K=0.21/yr, L_∞ = 60.0 cm, t-0 = 0/yr. The Growth parameters were K=0.18/yr, L_∞ = 61.1 cm, t-0 = -0.14/yr for golden gray mullet and were K=0.12/yr, L_∞ = 73.6 cm, t-0 = 0.92/yr for common carp. Based on catch-at-age data, in the years 2010-2011 and 2011-2012, the total biomass, from the biomass-based cohort analysis were estimated 41700mt and 34400 mt for kutum and 14600 mt and 14400 mt for golden grey mullet, respectively. The reference points of F0.1 and F35% were 0.41/yr and 0.34/yr for kutum and 0.36/yr and 0.33/yr for golden grey mullet, respectively. Stock enhancement plays an important role in recovery of kutum stocks in the Iranian coastal waters of Caspian Sea. There is a significantly negative correlation between fingerlings released and condition factor (CF) and recruitment and CF. The different trends for fingerlings, recruitments and CF suggest that CF may be partly density-dependent, declining at high population sizes due to intra-specific competition. Therefore, more research should be conducted to determine the desirable level of artificial propagation. Food consumed by fish species, Rutilus frisii kutum, Rutilus rutilus caspicus, Cyprinus carpio, Liza auratus and Liza saliens were included Foraminifera, Porifera, Annelida, Mollusca, Arthropoda, filamentous algae, fish eggs and detritus.The results showed that Rutilusfrisii kutum generally feed on Balanus and Cerastorderma. The main food item for Cyprinuscarpio, Liza auratus and Liza salienswas detritus. Based on available models, the ABCs were estimated as 6600-7400 mt for kutum and 2200-2800 mt for golden grey mullet (with precautionary approach 6600 mt and 2200 mt for kutum and mullet, respectively) in 2011-12. Two species (kutum and golden grey mullet) are vulnerable to environmental factors, and these factors should be considered in the stock assessment and management of the fish. For two species, the ABC with a lower andmore accurate value based onmore information, should be selectedfor the implementation of a precautionary management approach.
    Keywords: Fisheries ; Iran ; Caspian Sea ; Bony fishes ; Growth ; Mortality ; Biomass ; Acceptable biological catch ; Population ; Stock assessment ; Fishing ; Rutilus frisii kutum ; Rutilus rutilus caspicus ; Cyprinus carpio ; Liza auratus ; Algae ; Species
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  • 85
    Publication Date: 2021-07-16
    Description: This study was conducted to determine of phytoplankton abundance and diversity of water and their spatial and temporal fluctuations in the Mazandaran coastal of Caspian Sea in 6 months, at 4 transects (Tonekabon, Nowshahr, Babolsar, Amirabad) during different season of 2012-2013. 72 samples were collected at surface layer of water in 5, 15 and 30 m depths. The samples were analyzed based on the standard methods. 112 species contributed in phytoplankton community structure which where classified in 9 phyla namly: Bacillariophyta (42 species), Pyrrophyta (18 species), Cyanophyta (14 species), Chlorophyta (15 species), Euglenophyta (11 species), Cryptophyta (2 species), Chrysophyta (3 species), Haptophyta (1 species) and Xantophyta (1 species). Meanwhile small flagellate algae with Maximum Linear Dimension (MLD) 〈10 µ observed which they classified in small flagellates. Mean annual phytoplankton abundance with standard error obtained 164±32 million Cells/m^3. Seasonal study showed that phytoplankon abndance of summer was 1.5 folds of spring. The value in auttuman was same as spring, however it increased sharply in winter. The mean phytoplankton abundance of winter was 5 folds of the other seasons. Mean phytoplankton abundance of Tonkabon and Nowshahr (west transects) were 1.6 and 2 folds of Amirabad (east transect), respectively. Bacillariophyta with 89 percent of total abundance was the predominant phylum and Pyrrophyta was the second one. The third and fourth of dominant phyla were Cyanophyta and Chlorophyta, respectively. Chrysophyta and small flagellates showed equal percentage of abundance (1.4 percent of total abundance). Monthly study showed that Chaetoceros throndsenii was the first dominant species in Ordibehesht, Tir and Shahrivar. However, the first dominant species in Aban, Day and Esfand were Thalassionema nitzschioides, Skeletonema costatum and Pseudonitzschia seratia respectively. Mean phytoplankton biomass calculated 156.5 ± 18.1 during the study period. The mean of biomass was higher in summer and winter than the two other seasons. Phytoplankton biomass was formed mainly by Bacillariophyta and Pyrrophyta in all seasons. The highest biomass were belonged to Cyclotella meneghiniana and Coscinodiscus jonesianus (Bacillariophyta) in spring and summer respectively.While in fall and winter Prorocentrum proximum was in the first place of dominat species. Small size and flagellates species of different phyla (Chrysophyta, Bacillariophyt...) had importance role for determination of ecological and water quality conditions during spring to autuman. The increasing of phytoplankton abundance within these times indicates to regeneration of nutrients or entrainment of nutrient-rich deep water. Dominant species were observed in single forms, small filament and loose colonies during spring to autuman. However, these form shifted to fair-long chains form in winter which it indicates to nutrient-rich water was brought to the surface by vertical mixing process. It seems that environmental stress and instability of ecosystem was benefit to Chaetoceros throndsenii and Pseudonitzschia seriata which are known as species with bloom potential. Ability of reproduction in sewage environment (Chaetoceros throndsenii) and toxin production (Pseudonitzschia seriata) are the ecological and physiological significant characters of the two species.
    Keywords: Ecology ; Iran ; Caspian Sea ; Mazandran Proviece ; Tonekabon ; Nowshahr ; Babolsar ; Phytoplankton ; Abundance ; Biomass ; Biodiversity ; Species ; Sample ; Bacillariophyta ; Pyrrophyta ; Cyanophyta ; Euglenophyta ; Cryptophyta ; Chrysophyta ; Haptophyta ; Xantophyta ; Chaetoceros throndsenii ; Pseudonitzschia seriata
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  • 86
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25570 | 18721 | 2018-10-06 11:30:10 | 25570 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The aim of this study was comparison the effect of cow dung as organic fertilizer and chemical fertilizer on microbial flora, fish and human pathogenic bacteria and physico-chemical parameters of cyprinus fish pond water. The water samples were collected from two ponds, pond 1 was fertilized by cow dung and pond 2 was fertilized by chemical fertilizer, respectively from May to October 2011. . The aerobic and anaerobic bacteria were enumerated in TSA by serial dilution of the sample, followed by conventional pure plate method. Coliforms bacteria were similarly isolated on Chrom agarTM ECC. Moreover, the water samples were used to analysis of BOD, dissolved oxygen, conductivity, transparency, total alkalinity, total dissolved solid, total hardness, total nitrogen and total phosphorous. Temperature and pH were measured by thermometer and portable pH meter in place of ponds. For isolating of fish and human pathogenic bacteria, thirty six fish were collected from pond 1 from July to October and then sampled from their liver and kidney and inoculated in blood agar. The mean of total count of bacteria and total coliform count in cow dung sample were 4 × 104 ± 1200 and 4700 ± 300 CFUmL^-1 respectively. The mean of water total count bacteria of pond 1 were significantly higher than the water of pond 2. The water total count bacteria of pond 1 were significantly increased in August, but water total count of pond 2 was not shown significant variation from May to October. The results showed that Pseudomonas, Klebsiella, Enterobacter, Yersinia and E.coli were isolated from water of pond 1 but just Pseudomonas and E.coli were isolated from water of pond 2. Moreover, the human pathogenic bacteria shuc as Shigella and Salmonella were not isolated from of water and fish samples from pond 1. In this study, the bacteria growths were negative in all of the inoculated plate from fish liver and kidney. The total hardness, total phosphorus, total dissolved solid and conductivity of the pond 1 water were significantly higher than the pond 2 water. But the BOD, dissolved oxygen and transparency of the pond 2 were significantly higher than the pond 1 water. There were no significant differences in the total nitrogen, total alkalinity and pH between water of pond 1 and 2. In recent years, the chemical fertilizer has been used in cyprinus fish pond but many studies have showed that they were high risked as environmental pollution. Thus, the use of livestock manure especially cow dung could be a suitable alternative. The results showed the use of cow dung increased the bacterial population and diversity. Increasing of bacteria population caused increased total phosphorus that plays an important role in bloom of plankton because we found decreasing transparency and planktons are an important food for cyprinus fish. In despite of isolation of fish and human pathogenic bacteria from water, the bacteria growths were negative in all of the inoculated plate from fish liver and kidney. In summary, it seems the cow dung is a suitable alternative for fertilizing of cyprinus fish pond water if a microbial and physico-chemical monitoring of fish and pond water were continuously arranged.
    Keywords: Aquaculture ; Health ; Iran ; Pathogenic ; Bacteria ; Cyprinid ; Cow dung ; Fertilizer ; Microbial flora ; Cyprinus fish ; Dissolved oxygen ; Transparency ; Alkalinity ; Temperature ; pH ; Kidney ; Pseudomonas ; E.coli
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  • 87
    Publication Date: 2021-07-16
    Description: This study have been conducted entitle biological and statistical sturgeon in the southern Caspian Sea (Mazandaran waters)” from 2009 to 2013. Sampling was manthly. Overal, 1859 specimens of different species of sturgeon was caught including Acipenserpersicus (69%), A. stellatus (19%), Husohuso (6%), A. nudiventris (4%) and A. guldenstaedtii (2%). Totally, the meat and caviar of five species were found 53263.6 kg and 5633 kg respectively and caviar to meat ratio was 10.6%. The results show that ration of meat and caviar whole species reduced from 2009 to 2012 in which the meat and caviar of A. persicus was declined from 12146 to 4465 kg in meat and caviar from 1358 to 487 kg were extremely reduced respectively. The CPUE (catch per unit effort) of sturgeon has fluctuated from 2009 to 2012. Totally, the CPUE of A. persicus was decreased from 0.150 (2009) to 0.130 (2012) kg boat per day, A. stellatue from 0.009 (2009) to 0.021 (2012) kg boat/day, A. guldenstaedtii from 0.002 (2009) to 0.003 (2012) kg boat/day, A. nudiventris from 0.027 (2009) to 0.001 (2012) kg boat/day and H. huso from 0.123 (2009) to 0.018 (2012) kg boat /day. 1061 specimens of fish has been caviar including A. persicus with 69%, A. stellatus (18.1%), H. huso (5.5%), A. guldenstaedtii (2.7%). The frequency of caviar categorize was included 1 , 2, 3 and massive with 49.6%, 38%), 10.6% and 1.9% , respectively. The highest of meat and caviar belong to A. persicus with 31414 kg and 3515.7 kg, respectively were found and the lowest was 841kg and 105.9 kg pertain to A. guldenstaedtii. The results show that 65.9% of sturgeon was caught by legal catch (landing) and 34.1% by beach seine remained. 391 individuals was age determined that the minimum and maximum age was 9 and 41 year comprised to H.huso and A. stellatus, respectively. A. persicus and A. stellatus were majority caught compare to other species. A. persicus has the highest frequency in 14-15 year age groups with 50% and 57% in 2009 and 2011, respectively and 15-16 year age group with 41% and 56% in 2010 and 2012, respectively. The results show that mean of fork length, body weight, caviar weight of A. persicus was declined because the FL and weight average (±SD) obtained from 152.4±16.7cm and 25.8±9.2 kg in 2009 to 146.5± 18.7cm and 22.1±9.2kg in 2012, respectively. In contrast, the average (±SD) FL and weight of H. huso was increased from 218.8±41.1cm and 137.3±96.6kg in 2009 to 231.3±45.2cm in FL and 146.2±78.5kg body weight in 2012, respectively. Consequently, the results show that stocks of sturgeon in the Caspian Sea has a trend declined because of some species in IUCN categorize list was Endangered in 2006 but at this moment lie in Critically Endangered list of IUCN categorize were down which could be a serious alarm for the valuable sturgeon fish in the Caspian Sea. Therefore, recommendation that trade catch of sturgeon in the Caspian Sea must be forbidden and continue legal catch for spawner fish that needede to artificial propagation only.
    Keywords: Biology ; Ecology ; Iran ; Caspian Sea ; Mazandaran waters ; Biology ; Sturgeons ; A. persicus ; A. stellatus ; A. guldenstaedtii ; A. nudiventris ; H. huso ; CPUE ; Survey ; Sampling ; Species ; Caviar
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  • 88
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25587 | 18721 | 2018-10-07 11:26:14 | 25587 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This project was conducted in imam Khomeini mariculture research state from October 2010 to June 2013 to compare reproductive performance between wild and domesticated Sobaity seabream broodstocks. In 3 years wild broodstocks did not spawn in 5 and 10 fiberglass and concrete tanks but domesticated broodstocks spontaneously spawned from February to March. In the first year domesticated broodstock in ratio of 3 females to 6 males allotted in 10 mt concrete tanks in triplicate. the about 2776000 eggs produced from 9 females and 18 males but further heavy mortality occurred because of winter shock syndrome and suppression of immunity system. Mean temperature, salinity and PH in incubation period was 16.5˚C (range between16-18˚C), 45ppt, 8.5 respectively. Mean floating eggs and hatch percent was 15.5% and 8.9% and about 19000 larvae produced. In the second year domesticated broodstock in ratio of 9 females to 23 males allotted in 5 mt fiberglass tanks in triplicate .about 17540000 eggs acquired from 27 female and 69 male broodstocks. Mean temperature, salinity and PH in culture period was 24.8 ˚C(range between20-28.3˚C), 47.5 ppt, 8.4 respectively. Mean floating eggs and hatch percent was 58.94% and 84% and about 8640000 larvae produced and stocking density was 10-30 larvae per liter. After 61 days mean production and survival were 503.3 (range between 140-1220) larvae per cubic meter and 5.3% (range between 0.47–12.2 %) respectively. Final mean weight of fry was 2.6 gr. In the third year domesticated broodstock in ratio of 3 females to 6 males allotted in 5 mt fiberglass tanks in triplicate. about 6570000 eggs acquired from 9 female and 18 male brood stocks. Mean temperature, salinity and pH in culture period was 23.9˚C(range between 19.5-28.4˚C), 45, 7.6 respectively. Mean floating eggs and hatch percent was 54.7% and 81.6% and about 2889800 larvae produced and stocking density was 10-30 larvae per liter. At the end of 87 and 89 days of husbandry the mean production and survival were 359 (range from 300 to 397) larvae per cubic meter and 2.7% (range between 1.32–3.8 %) respectively. Final mean weight of fry was 3.5 gram and Larvaeculture in the last 2 years carried out in 5 cubic meters fiberglass tanks. It seems to domesticated broodstocks because of spontaneous spawning, long spawning period, more brood stocks and possibility of reusing them in the next year are very economical for propagation than wild broodstocks.
    Keywords: Aquaculture ; Iran ; Sparidentex hasta ; Fry production ; Spawning ; Larvae culture ; Propagation ; Sobaity ; Sparidentex hasta ; Rearing ; Mariculture ; Broodstocks ; Temperature ; Salinity ; pH ; Survival
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  • 89
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25589 | 18721 | 2018-10-07 10:35:13 | 25589 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The study was done in Nutrition and Live Food Station was located in Bandar Anzali Ghaziyan. Juveniles weighted average 0.5, 1, 2.5, 5, 10, 15 and 20 g were randomly selected in three water conditions with a salinity of 11 ppt (Caspian sea water), water 7 ppt and freshwater (with three replicates per group) were included. At intervals of 0, 3, 6, 12, 24, 72, 168, 240 hours, blood samples were heparinized capillary tubes by caudal juveniles and ion concentrations Mg, Ca,Cl using the spectrophotometer and sodium and potassium ions with Flaym photometry (flame photometric), the osmotic pressure of blood plasma by osmometer and cortisol levels were measured by RIA method. To study the microstructure of gill and kidney tissue for each treatment , tissue samples by classical histological methods and stained with hematoxylin - eosin slides were prepared. The frequency and location of the enzyme Na^+, K^+ - ATPase and chloride cells with immunohistochemical localization was performed. Studies micrometric gill chloride cells and renal glomerular networks by software Image tool (version 2.0) was performed. Measurement of enzyme Na^+, K^+-ATPase, by Zaugg (1982) method was conducted. Data analyzed by one-way ANOVA (Oneway ANOVA) with Tukey's test was performed. Overall, the results of measuring ions and osmotic pressure on the tenth day of treatment, the osmotic potential juveniles 2.5, 5, 10, 20 gr in Caspian sea water and all groups except the 0.5 in water of 7 ppt confirmed. But in case of unfavorable conditions for the release in estuaries river and river, fish with weight 1 to 3 release directly to beach (where the salinity is 7 grams per liter) and fishes with weight from 10 to 20 gr to sea. Although suitable river conditions necessary condition for release of juveniles in riverine areas to adaptation juveniles occur gradually.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Rutilus frisii kutum ; Osmolarity ; Suitable size ; Release
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  • 90
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25631 | 18721 | 2018-10-07 16:55:18 | 25631 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Rutilus kutum is one of the most important economic fish species of Iranian costal of Caspian Sea. This species conducted more than 60% of fish caught in Caspian Sea annually, and more than 10 thousands fisherman engaged to its fishery. In present study, kutum fingerlings reared in two different densities include of 25 and 40 fish/m^2 with mean weight of 17.5±5.87 g. The period of test being planned 10 weeks and the test organism housed in circular concrete pools of 3 m^3 capacity with flowing water and aeration in pools are supplied. The fishes feed by extrude food with 45% crude protein and 14% crude fat. After termination of the experiment, the result showed that weight gain performance, specific growth rate, condition factor, food efficiency rate and protein efficiency rate decreased, while food conversion ratio increased with rising of density, as their different was significance in two experimental treatments (p〈0.05). The kutum reared in low density showed significantly higher survival rate (SVR) (with 88.8%) than high density (with 76.9%) (p〈0.05). Based on the different observed effects of the density on growth and survival, it is recommended the use of a 25 kutum/m^2 density for rearing by Caspian Sea water.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Rutilus kutum ; Density ; Culture ; Growth ; Survival ; Fishery ; Fingerlings ; SVR ; Rearing
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  • 91
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25626 | 18721 | 2018-10-08 07:10:37 | 25626 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Study of tagging effects on survival rate of Caspian trout (Salmo trutta caspius) fries before releasing to natural habitat was a part of a large research plan on conservation and restoration of Caspian trout stocks in the Southern Caspian by Coldwater Fishes Research Center. Catching brooders, propagation, larvae and juvenile rearing were carried out in Southern coasts and rivers of Caspian sea and in shahid Bahonar propagation center in Kelardasht, Mazandaran (North of Iran) in autumn 2012. 150 fries for each treatment (5, 10 and 20 g fries and Plastic T tag, elastomer tag and control treatments) anesthetized, weighed (g), measured (mm), tagged, disinfected and introduced to cement ponds. Fries were kept and fed for 5 days and their behavior and responses to food, infection of wound and mortality were recorded. No mortality were observed in all treatments, fries were shocked for few hours after handling and did not take food but they started to feed from the first day after tagging and there was no infection in macroscopic observation of wound.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Mazandaran Province ; Kelardasht ; Caspian trout ; Salmo trutta caspius ; Tagging ; T tag ; Elastomer tag ; Surviving Rate ; Fries ; Natural Habitat ; Survival rate ; Coldwater ; Propagation ; Larvae ; Juvenile ; Rearing ; Mortality
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  • 92
    Publication Date: 2021-07-16
    Description: This study aimed to investigate the effects of 5- parameter dissolved oxygen, PH, nitrite, nitrate and temperature of the water on Streptococcosis incidence in two groups of fry and grower fish. Research was performed at west Mazandaran province -Tonkabon region in 12 rainbow trout selected farms. Research conducted in 3 farms with well source water, 8 farms with water source of the river of Dohezar and one farm with water source of the river of Azarood, during 12 consecutive months. From 1390.04.01 to 1391.04.01, once time each month, and in each time 10 fish randomly sampled, inlet water were sampled simultaneously Of 1350 sampled fish 607 fish with an average weight 22.04 gr, average length 12.59 cm were in fry category and 743 fish with an average weight 156.25 gr, average length 23.32 cm in were grower category. Streptococcosis observed only in grower category Of 72 fish with clinical signs of the disease, 14 numbers were positive Streptococcosis (19.44%) and 58 numbers were negative Streptococcosis (80.55%). Three fish from grower category has not any clinical signs of disease and seemed to be healthy but were positive Streptococcosis in examinations (0.22% of total fish sampled. Fish with clinical signs of the disease but negative Streptococcosis were of at least 55.1 gr and at most weight 417 gr respectively. The results showed that 47.07% cases of Streptococcosis happened at 15.6°C water temperature, 35.29% at 16.98°C and 17.64% cases happened at 18.04°C so that 100% of Streptococcosis cases was observed at the average temperature of 16.99°C. In addition, the survey results show that despite relatively high levels of nitrite in source water of farms from wells, nitrite does not effect on the disease incidence. It seems that an optimal level of dissolved oxygen in water is effective in reducing the effectiveness of nitrite in this disease. According to equation coefficients logit model is as follows: According to Logit model, it seems that for every degree change in temperature and pH of water, morbidity change will diminish 0.37 % and 1.74 % respectively. 80.56% of fish sampled that had the clinical symptoms, was ranging from an average weight of 5.1 gr (fry) and 417 gr (grower fish), that despite having clinical signs of the disease were negative Streptococcosis. Isolation of Staphylococcus bacteria as well as Gram-negative bacilli from fish with clinical symptoms similar to the symptoms of Streptococcosis, may indicate the involvement of other pathogens in fish clinical signs.
    Keywords: Ecology ; Iran ; Mazandaran Province ; Tonkabon ; Streptococcusis ; Risk factors ; Cold water fish ; Survey ; Rainbow trout ; Dissolved oxygen ; pH ; Nitrite ; Clinical signs ; Temperature ; Pathogen
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  • 93
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25620 | 18721 | 2018-10-14 02:36:27 | 25620 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The aim of this study was to find the best location for establishment of rainbow trout culture and hatcheries farms on Zayanderood’s river region in Charmahal and Bakhtiyari province. This survey carried out over ten station along Zayanderood’s river. The result of physical and chemical analysis showd that the annual average of air temperature varied from 9.5oC - 10oC where the pH annual average value were between 7.5 and 8.8. the dissolve oxygen concentration in stations except in rainbow trout farm effluent were above 10 mg/l. the other chemical , pollutant as well as pesticides levels were under the limiting concentration for rainbow trout culture and Hatcheries activity. The plankton survey showed that the Bacillurophyta were the dominant group of phytoplankton where protozoa constituted the most abundant group of zooplankton the Benthic organisms sensitive to pollutant in particular Epirus were dominant group in all stations. In regard to fishes presence in river, five species of Ciprinidae, one species from Balitoridae and one species from salmonidae families were identified. The capacity for development of rainbow trout culture for tow phase period in Zayanderood’s river region with respect to self purification potential (self purification potential were determined from the oxidation of the effluent of the only active trout farm of the river region), minimum of 10 L/s water requirement for production of trout in concrete canal and pond system and 1 L/S water need for production in semi circular closed system were estimated to be 5202 metric tons.
    Keywords: Aquaculture ; Iran ; Caharmahal and Bakhtiyari province ; Positioning ; Rainbow trout ; Self purification ; Culture ; Hatchery ; Survey ; Temperature ; pH ; Dissolved oxygen ; Pollutant ; Plankton ; Bacillurophyta ; Phytoplankton ; Protozoa ; Zooplankton ; Benthic organisms ; Ciprinidae ; Balitoridae ; Salmonidae
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25637 | 18721 | 2018-10-08 08:10:22 | 25637 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Nutrients, organic matter and benthos communities of sea bed, health and growth of Staghorn corals (Acropora spp.) were investigated at two stations on the Lark Island from Jul.2012 to Feb.2013 seasonally. ANOVA test was used for the spatial and temporal variations in the studied between stations and seasons (95 percent confidence interval). Also, Principal Component Analysis (PCA) factor was used in order to identify the most important parameters influencing of coral growth. ANOVA test indicated difference between Biometrics new coral colonies at both the station and the old coral colonies in the West Island Station Lark in the summer and spring together with autumn and winter(P〈0.05), Although there was no significant difference between autumn and winter(P〉0.05). Salinity factor was similarity (P〉0.05), but the factor of temperature was difference in the summer with other at both stations in all seasons (p〈0.05). Diving observations indicated that adaptations of in the coral habitat at both stations is selected. In order to avoid of stress caused by fluctuations in temperature and salinity at both stations, Staghorn corals were distributed in the region away from the coastline. Also, these communities have chosen transverse expansion strategy a way to escape the stress of sea surface temperature fluctuations. Significant correlation was reported between temperature fluctuations and the development of the Lark island's coral bleaching. It also has been reported to the Persian Gulf corals are vulnerable to unusual changes in temperature and long term stress. Studied nutrients (nitrate, nitrite, phosphate and silicate) in front of Lark station had a higher amount of station of West Island. The results of nutrients (nitrate, nitrite, phosphate and silicate) did not show any difference in the previous studies in the larak coral ecosystems. Therefore, the probability of coral loses in front of Larak station, caused no recycling of materials, and increased accumulation of nutrients in the current situation. Benthos communities of bottom were differed (P〈0.05), but the density of these communities, there were no differed in the two stations in the same season (P〉0.05).The study of Gran size of seabed was showed the highest amount of sand in all seasons at both stations. Studies showed that, in the absence of environmental stress, the type of bottom sediments had as an important factor in the distribution and abundance of benthic organisms. Although coral life is not directly related to the richness of the seabed, but production in marine ecosystems directly and indirectly related to these communities, which is influential on the richness of these ecosystems. In studying Coral Watch method were concluded difference between indices used in seasons of summer and winter together, and with autumn and winter seasons (p〈0.05), although no differences was observed between autumn and winter(p〉0.05).The indices obtained in this study can be concluded the minimum stress during the period of the study was dominated ecosystems, also difference between summer and spring of this study could have been influenced by the effect of temperature on zooxanthella concentration. Two components were identified in this study using of principal component factor analysis to identification the most important parameters that influence the growth of corals. Also, the variability of each factor studied was different.Highest loading factor on the first component of turbidity, temperature and salinity, and the second component loading factor maximum nitrate, nitrite and phosphate are inversely associated with coral growth. According to consideration the ecological conditions were determined two area to rehabilitation stocks of Staghorn corals around the Lark Island by using ArcGis software.
    Keywords: Aquaculture ; Iran ; Persian Gulf ; Larak Island ; Rehabilitation ; Coral stock ; Acropora spp. ; Nutrients ; Organic matter ; Benthose ; ANOVA ; Growth ; Temperature ; Zooxanthellae
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25644 | 18721 | 2018-10-08 08:25:33 | 25644 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The Gorgan Bay has situated in the South east of the Caspian Sea and water entersthe Bay from the Sea .it covers an area about 450 squar kilometers that is connected to the sea only by the passage of Ashouradeh in the north east of the bay . The water balance is almost constant and is not tidal. In this study, a model made for the bay on the basis of its water quality and quantity. Its reactions evaluated under different qualified scenarios and its surrounding water quality survey during next 29 years. Mike 21 software was used for 2 dimensional modeling . At first hydrodynamic modeling of the bay was done .one of the important factor was water depth which calculated by hydrography method of the bay. Then, a model made for the salinity of the bay. Since, salinity is a stable parameter, a sub-program TM (Mike 21) which has the ability to make a model from the process of advection – dispersion was used. The amount of Manning (0.025) and Dispersion (50) coefficient calculated simultaneously with calibration of salinity model (D:square meter / second). Results from data verification of salinity model indicated about 3 percent mean error. The pollution dispersion type in the bay evaluated at six points of the bay using the method of dot constant source of pollution. Results showed that around 99.9 percent of pollution decreases under the effect of dilution process till the 100 m distance. Then modeling of dissolved oxygen, Ammonia, nitrite and nitrate was done by using of subprogram ECOLAB at the Mike software To make these models, first the sensitivity analysis of these parameters was done. Important parameters chose for model calibration and then data verified and also the model calibrated. The result of data verification test showed the mean error of oxygen, ammonia, nitrite and nitrate were respectively about 3, 4, 6.3 and 37 percent. Then modeling made according to four different scenarios which defined on the basis of pollutions originated from aquaculture in the bay (scenario 1: aquaculture at the first half year. Scenario 2: aquaculture at the 2nd half year. Scenario 3: aquaculture at the whole of year. Scenario 4: cultivation of bluga for 3 years). Water quality of the bay and also different amount of aquaculture production surveyed during next 29 years under different scenarios of aquacultures. The places under pollution were included the appropriate places for aquacultures in each scenario that was already determined at the bay .Then the reaction of the Gorgan bay was determined under each scenario. The curves of pollution fluctuations were drawn along with amount of cultivation at the each scenario. At last, appropriate amount of cultivation was calculated at each scenario with considering of self-purification of the bay that does not tend to pollution of the bay. On the other hand, the farms arrange, distances between farms and the number of cultivation ponds at the each farm was calculated. Finally, results showed that the aquaculture capacity of the bay is less than 300 tons per year.
    Keywords: Aquaculture ; Ecology ; Iran ; Caspian Sea ; Gorgan Bay ; Aquaculture ; Salinity ; Pollution ; Dissolved oxygen ; Ammonia ; Nitrite ; Cultivation ; Beluga ; Self purification
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25643 | 18721 | 2018-10-14 02:41:14 | 25643 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The project investigates the relationship between the biological parameters (phytoplankton, zooplankton, Macrobenthic and ctenophore- Mnemiopsis leidyi) and environmental parameters, nutrients and environmental pollutants (oil, pesticides, heavy metals, and detergents) in water and sediment, at the southern Caspian Sea in 2010-2011. Sampling was carried out in four seasons (spring, summer, autumn and winter) and in eight transects perpendicular to the coast (Astara, Anzali, Sefidroud, Tonekabon, Noshahr, Babolsar, Amir Abad and Bandar Turkmen). Samples were collected from the different layers at depths of 5, 10, 20, 50 and 100 meters. The relationship between biological and environmental parameters surveyed through parametric and multivariate statistical methods. Result showed that the annual mean of environmental parameters and nutrients concentration such as water temperature, pH, transparency, DO, ammonium, nitrate, inorganic nitrogen (DIN), organic nitrogen (DON), inorganic phosphorus (DIP), organic phosphorus (DOP) and soluble silicon (DSi) at euphotic layer were 16.70±0.43 (ºC), 8.38±0.01 (m), 5.48±0.05 (ml/l), 1.52±0.06 (µM), 1.80±0.08 (µM), 3.41±0.10 (µM), 43.3±0.9 (µM), 0.32±0.01 (µM), 0.52±0.02 (µM), 8.88±0.22 (µM), respectively. Meanwhile, annual mean of environmental pollutant such as PAHs and OCPs in sediment were recorded 0.88±0.16 (µg/g.dw) and 9.78±2.20 (µg/g.dw), respectively. In addition, annual mean of heavy metals such as Zn, Cu, Ni, Pb and Hg in sediment were obtained 247±46 (µg/g.dw), 29.5±1.5 (µg/g.dw), 49.9±4.9 (µg/g.dw) and 0.179±0.800 (µg/g.dw), respectively. Annual mean abundance of biological parameters namely phytoplankton, zooplankton and M. leidyi (0-20m) at photic layer were 238±17 (million cells/m^3), 4808±362 (individuals/m^3) and 26±3 (individuals /m^3) respectively, and for biomass were 747±60 (mg/m3), 44.3±5.0 (mg/m^3), 2.15±0.31 (g/m^3). Annual mean abundance of those biological parameters at below of photic layer (50-100m) were 104±35 (million cells/m^3), 843±92 (individuals/m^3) and 2±1 (individuals /m^3) respectively, and for biomass were 412±93 (mg/m3), 9.1±1.0 (mg/m^3), 0.15±0.05 (g/m^3). Annual mean abundance and biomass of macrobenthic were 5073±1225 (individuals /m^2) and 144±73 (g/m^2), respectively. Annual mean annual percentage of TOM, Gravel, Sand and Silt-clay were recorded 3.74±0.26, 0.92±0.32 , 22.51±4.97 and 76.67±5.01, respectively. The stratification of water column was strongly based on gradient of water temperature and the phenomenon (difference of temperature between water layers) was more clear in this study compared to previous years. Temperature and biological factors (phytoplankton) were effected on changes of dissolved oxygen at warm and cold seasons summer and winter), but coefficient factor of temperature was higher than biological factors in winter. The nutrients concentration (with the exception of inorganic phosphorus) in different years 2008-2009, 2009-2010 and 2010-2011 increased compared to 1995-1996 (the year of stability of ecosystem). One of the reason attribute to the presence of the ctenophore (M. leidyi) in Caspian Sea after 1999. The annual correlation of phytoplankton abundance and temperature was reversed but seasonal pattern was varied at each season (within a year). In this study, the Caspian Sea contained the conditions of nitrogen limitation (55%) and nitrogenphosphorus limitation (6-43%) as well as phosphate limitation (2-39%) (DIN/DIP〉20) . Inspite of no silica limitation (sufficient concentration of silica) in the Caspian ecosystem, Bacillariophyta was not dominance phylum at whole seasons.It seems that other factors such as the temperature changes of seasons, the effects of predation and feeding of the next chains of the food chain, the difference of the ability in the growth and reproduction, competition (uptake of nutrients) in dfferent groups of phytoplankton and stoichiometry of the nutrients (nitrogen and phosphorus) were caused of non-diatoms dominance at most seasons. As, Pyrrophyta and Bacillariophyta were dominant at spring and winter, respectively and Cyanophyta was pre-dominant at summer and autumn. Multivariate analysis showed the significant correlation between Coppepoda and oxygen and water temperature only. The other gropus of zooplankton did not show any significant correlation with environmental parameters. It might be due to stronger effects of other parameters such as food and predators on different groups of zooplankton at each season and abundance of zooplankton groups indirectly affected by environmental parameters. In this study, Shannon diversity indices of zooplankton and phytoplankton were closer to 1995-96 values and showed diferent trend compared to 2009-2010. However it is not enough reason for recovery of ecosystem in to the stability of Caspian Sea. It is because of other negative evidiance such as strong increasing trend of phytoplankton to zooplankton biomass ratio in all seasons and regions particularly the 2009-2010 and 2010-2011 years compared to 1995-96 (the year of stable ecosystem). In the other word, the balance between the biomass of the first and second of the food chain has been disturbed and the value was much much higher than the year of stable ecosystem in 1995-96. Based on multivariate analyses, there was not significant correlation between zooplankton groups and some edible phytoplankton species, vise versa zooplankton groups consumed some unsuitable species of phytoplankton (based on size, nutritional value, difficulty of digestion and absorption, the potential of toxicity and harmfulness). The lack of expected relationship and routine rules of nutritional between zooplankton and phytoplankton are the more resons of instability in the ecosystem. In current study, dominant group of macrobenthos (polychaeta) observed in depths less than 20 meters which the percentage of silt-clay and sand were 74 and 26, respectively. It seems that this ratio of silt-caly and sand was suitable for their living and accumulation. PCA analysis showed that increasing the percentage of TOM and siltclay accompanied to the decreasing of macrobenthos abundance while increasing the temperature, dissolved oxygen and pH had a positive effect on macrobenthos abundance in most seasons. Increasing the abundance of macrobenthos at all seasons (except spring) would not be a strong indication of improvement of Caspian ecosystem after the ctenophore introduction stress and unfavorable evidence such as low Shannon diversity index observe in the results. Meanwhile, in the present study, Streblospio and oligochaeta (invasive growth and advantage to the food uptake and habitat and sediment seeding) similar to the years of 2008-2009, 2009-2010 still were dominant groups insteade of Gammaridae family (feeding on suspended solids). This means that sediment has a noteworthy amount of organic matter which indicate to the trophic level of ecosystem tend to eutrophy level. The comparison of results on this study to previous studies on biological parameters (phytoplankton, zooplankton and macrobenthos) indicating to the persistence of stress (such as biological and anthropogenic) on their changing population patterns (quantitative relationships between species) and structural patterns (species composition and seasonal succession of dominant species). In other words, many species (both macroscopic and microscopic) of the Caspian Sea are still vulnerable to complications of stressor factors. In order to protection and sustainable exploitation of this worth ecosystem it is necessary to look more serious studies and practical techniques from the relevant organizations in this area.
    Keywords: Ecology ; Pollution ; Iran ; Caspian Sea ; Astara ; Anzali ; Tonekabon ; Noshahr ; Babolsar
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25652 | 18721 | 2018-10-08 08:43:54 | 25652 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: For the first time white spot disease (WSD) was reported in shrimp farms of khoozestan province, in southwest of IRAN in 2002. Then in 2005 the neighbor province, boushehr, was contaminated. In 2008 WSD outbreak reported in sistan-bloochestan province in southeast of Iran. In 2015 all of southern shrimp farms of country except Hormozgan, the middle southern province, which has remained free of WSD, are being contaminated. White Spot disease suspended shrimp culture in thousands hectares of shrimp farms. Considering that white spot disease has not been observed in Hormozgan province yet, the question is; to what extent environmental and management factors participated in preventing WSD outbreak or cause WSD outbreak. In this study (20102012), the effects of environmental factors and management, stressors that decrease immune system function of shrimp are discussed. In addition, the role of pathogen as the main factor of outbreak is discussed. The goal of this study is to define environmental parameters and management practices associates with outbreak of white spot disease in affected provinces and discover reasons of being Hormozgan province free of this disease. In this study the role of the local environmental factors and management practice stressors in susceptibility to WSD was determine. Both the effects of environmental factors in water of ponds including total ammonia, nitrogen, dissolved oxygen, pH, salinity, transparency, and temperature and management issues related to biosecurity are studied. There were overlaps on physical and chemical parameter values obtained in clear areas with contaminated areas .Results of the data analysis suggest that lack of association with WSD incidence was 7 times greater than WSD incidence despite of disease outbreak in sistan-bloochestan province, so other sources of white spot disease virus incidence was suspected in affected areas. Histopathological examinations and polymerase chain reaction (PCR) tests during project performance did not reveal white spot disease virus evidences in post larvae examined from khoozestan province stocked in farms but disease outbreak was happened in that farms , so we suspected to management practice include feed , pond preparation and carrier of disease. Recorded values of temperature and salinity in some months during inspection in Hormozgan province specified stressful condition that may lead to WSD outbreak, however the disease did not appear. Therefore the hypothesis that the water physical and chemical conditions are reasons to prevent disease outbreak in Hormozgan province is being rejected. The policy of Hormozgan’s fishery authorities, to replaced Fenneropenaeus indicus with specific pathogen free Litopenaeus vannamei, that is more resistant to some of diseases, before incidence of WSD in farms and to before being endemic in the Hormozgan province, made an advantage compare to affected southern provinces that introduced Litopenaeus vannamei after WSD prevalence to their farms. However it does not guarantee to maintain current trend of being Hormozgan province farms free of white spot disease. Therefore establishing the principals of biosecurity are strongly emphasized. Strategies taken by the proficient authorities in preparation of SPF shrimp broodstock can be the most important factor in preventing WSD. Regarding biosecurity principals purchased feed must be free of shrimp head powder. Construction the new shrimp farms should be as far as it could be away from contaminated areas.
    Keywords: Aquaculture ; Ecology ; Health ; Iran ; Khouzestan Province ; Boushehr province ; Sistan and Balochestan province ; Hormozgan Province ; Epidemiological study ; WSD ; Fenneropenaeus indicus ; Penaeus vannamei ; Shrimp ; White Spot Disease ; Temperature ; Salinity ; PCR ; Polymerase Chain Reaction ; Ammonia ; Nitrogen ; Dissolved oxygen ; pH ; Transparency
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25657 | 18721 | 2018-10-08 08:23:30 | 25657 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was conducted to spatio-temporal fluctuation and distribution of phosphorous and nitrogen species, total organic carbone and some environmental parameters at suface sediments in the southern of Caspian Sea. In current study, 48 samples were collected from four stations (Bandar Anzali, Tonekabon, Bandar Nowshahr and Bandar Amirabad) and three dephths (5, 10 and 20 meter) during four seasons in 2013-2014. Results of this study showed that annual mean of Adsorbed-P, Fe-P, Al-P, Bioava.-P, Ca-P, TIP, Rese.-P and TP were obtained 6.68±0.63, 60.90±2.44, 24.94±2.39, 94.52±3.62, 1868±36, 1963±37, 87.51±9.52 and 2050±38 µg/g.dw, respectively. Result also showed that inorganic phophotous was maximum during different seasons, however, percent mean of residue-P which containe organic compounds and non-degrediable compounds, was less that 5 percent. Percent of Ca-P was higher than 90, whereas Bioava.-P was less that 10 percent. The order of different forms of phosphorous were registered Ca-P〉Org-P〉Fe-P〉Al-P〉Adsorbed-P.Annual mean of NH4/N, NO3/N, TIN, TON and TN were observed 2.56±0.27, 0.89±0.12, 3.52±0.26, 6.06±0.27 and 9.57±0.32µg/g.dw respectively. Annual percent of TON was two folds than TIN and NH4/N was also three times than NO3/N. In addition, mean of Fe, Al, Ca, Eh and TOC% were obtained 25.39±0.65, 18.76±0.68, 60.15±1.50 mg/g.dw and 67.02±0.68, 1.11±0.09, respectively. Maximum mean of carbon (1152±171), nitrogen (0.87±0.05) and phoporous (74±23) µM/g were observed in fall, winter and summer, recpectively. Also, maximum ratios of TOC:TP (16.8±2.3) and TOC:TN (2378±363) were obtained in fall season. As a conclusion, mian role of Adsorbed-P, Fe-P and Al-P adsorbed and desorbed were temperature, Eh and pH, respectively. Results of this study showed that TOC:TN was very high which represented to excitance of organic matter with almost non-degrediable. Whereas, TOC:TP was low which showed that organic phophoros was not related to proteins and phospholipids therefore sources of org-P were belong to other compounds.
    Keywords: Ecology ; Iran ; Caspian Sea ; Anzali ; Tonekabon ; Nowshahr ; Phophorous ; Carbon ; Nitrogen ; Environmental parameters ; Surface sediments ; Nutrients ; Algal bloom ; Species ; Samples ; Temperature ; pH ; Proteins ; Phospholipid
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  • 99
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25670 | 18721 | 2018-10-08 08:56:10 | 25670 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The lack of sustainable management programs has resulted in the drastic decline of sturgeons stocks in the Caspian Sea in the past two decades. Legal catch quotas for all Caspian littoral states has dropped from 28500 tons in 1985 to 460 tons in 2007 while caviar production in the Caspian Sea during the same period decreased from 3000 tons to 70 tons. Caviar production in the I.R. of Iran dropped from 305 tons in 1985 to about 11 tons in 2007. The "Strategic and applied research planning for sturgeon management and conservation" was developed with the collaboration of academicians from universities, researchers, experts, fishery authorities and representatives of the executive government to maintain sustainable development and rational management of sturgeon stocks in the Caspian Sea. The preparation and development of this planning was carried out through 121 sessions (2100 person hours) A comprehensive report (375 pg) was prepared which was divided into five volumes; Volume I comprises an introduction, objectives, strategies and planning, present status of production and caviar harvest, catch and export quotas, revenue generated and release of sturgeon fingerlings, Volume II comprises analysis of the problem by constructing a problem tree with 8 broad categories to analyze 344 problems and an objective tree which is the hierarchic flowchart of objectives with 9 broad categories with 241 items, Volume III comprises a review and analysis of previous and ongoing research (414 projects) on different aspects of sturgeon during the past 40 years, Volume IV comprises prioritizing research objectives outlined by the objective tree and finally Volume V which prioritizes primary objectives for 14 executive and 10 research programs. On the basis of the evaluation of the present status and for the sustainable development and rational management of sturgeon stocks a strategic and applied research planning program was proposed and developed within the framework of three primary objectives; 1) Management and sustainable use of Caspian Sea resources (5 programs), 2) Rehabilitation and restoration of stocks (5 programs), 3) Development of aquaculture (4 programs). The applied research program for each proposed executive program was outlined in 10 programs, 42 comprehensive plans and 222 projects. The primary objective on management and sustainable use of Caspian Sea resources includes 5 programs, 14 comprehensive plans and 63 projects, while the primary objective on rehabilitation and restoration of stocks includes 4 programs, 16 comprehensive plans and 87 projects. The primary objective to address sturgeon aquaculture includes 1 program, 12 comprehensive plans and 72 projects. The impacts and outcome of each of these programs was determined and presented. It is evident that if the present situation persists, the catch figures for adult sturgeon specimens and caviar production in Iran will reach zero in 2021. But if concerted efforts are taken and the proposed strategic and applied research planning program is executed (commencing from 2009) we can put a halt to these declining trends. By producing sturgeon fingerlings to restore population abundances, by conserving and protecting them in the Caspian Sea and by conducting applied research we can produce 206.4 tons of caviar by the year 2033. Apart from harvesting caviar from the Caspian Sea we can also annually produce 3000 tons sturgeon meat and 60 tons farmed caviar starting from the year 2023. The total budget proposed for implementation of this program for a period of 14 years (2008-2022) is USD 2483 million. If this budget is allocated in time and the proposed strategic program is properly and totally implemented, we can not only save many sturgeon populations from extinction but also ensure job opportunities for 6000 fisherman, provide 1480 new job opportunities and annually produce 266 tons of caviar which will generate USD 4957 million.
    Keywords: Ecology ; Iran ; Caspian Sea ; Guilan Province ; Mazandaran Province ; Golestan Province ; Sturgeons ; Caviar ; Strategic ; Fingerlings ; Survey ; Fisheries
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25679 | 18721 | 2018-10-08 09:40:22 | 25679 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: From an environmental standpoint bays around the world are very important ecosystems including the Gulf of Gorgan Bay and thus are important. Which has a relatively low depth, a little flow, temperature appropriate, biogenic materials and abundant food in the sea suitable for children sometimes place (Nursery ground) and also the perfect place for power (Fattening ground) is an early sea fish. In this research effort will be the initial studies on the impact of environmental pollutants on the ecosystem of the Gulf of Gorgan and the quality and quantity of pollution, especially pollution, agricultural pesticides and entering the Gulf of Gorgan and the impact on the ecosystem's the initial assessment and the monitoring of changes in them over the years. The user in the Gulf of Gorgan, agriculture. Pollution from agricultural fields related to pesticides and fertilizers are used in the fields. According to consumer research, and the remaining amount of pesticides in water and sediments of the Gulf of measured, indicated that most of the remains of chlorinated pesticides in sediments of the pesticides used in the agricultural sector In addition, all the toxins in the summer and spring peak. In addition, the DDE concentration compared to other poisons. In the case of other contaminants in the area is now threatened by the Gulf of Gorgan, although not serious, but it seems that the increase in organic matter and reduce the amount of oxygen of the water. The near future to Eutrofication risks in the ecosystem.
    Keywords: Pollution ; Iran ; Gorgan Bay ; Pollution ; Ecosystem ; Temperature
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