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  • American Meteorological Society  (124)
  • Nature Research  (112)
  • American Institute of Physics
  • MDPI Publishing
  • Nature Publishing Group
  • Public Library of Science
  • 2020-2023  (264)
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  • 1
    Publication Date: 2022-05-25
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Malige, F., Patris, J., Buchan, S. J., Stafford, K. M., Shabangu, F., Findlay, K., Hucke-Gaete, R., Neira, S., Clark, C. W., & Glotin, H. Inter-annual decrease in pulse rate and peak frequency of Southeast Pacific blue whale song types. Scientific Reports, 10(1), (2020): 8121, doi:10.1038/s41598-020-64613-0.
    Description: A decrease in the frequency of two southeast Pacific blue whale song types was examined over decades, using acoustic data from several different sources in the eastern Pacific Ocean ranging between the Equator and Chilean Patagonia. The pulse rate of the song units as well as their peak frequency were measured using two different methods (summed auto-correlation and Fourier transform). The sources of error associated with each measurement were assessed. There was a linear decline in both parameters for the more common song type (southeast Pacific song type n.2) between 1997 to 2017. An abbreviated analysis, also showed a frequency decline in the scarcer southeast Pacific song type n.1 between 1970 to 2014, revealing that both song types are declining at similar rates. We discussed the use of measuring both pulse rate and peak frequency to examine the frequency decline. Finally, a comparison of the rates of frequency decline with other song types reported in the literature and a discussion on the reasons of the frequency shift are presented.
    Description: The authors thank the help of Explorasub diving center (Chile), Agrupación turística Chañaral de Aceituno (Chile), ONG Eutropia (Chile), Valparaiso university (Chile), the international institutions and research programs CTBTO, IWC, BRILAM STIC AmSud 17-STIC-01. S.J.B. thanks support from the Center for Oceanographic Research COPAS Sur-Austral, CONICYT PIA PFB31, Biology Department of Woods Hole Oceanographic Institution, the Office of Naval Research Global (awards N62909-16-2214 and N00014-17-2606), and a grant to the Centro de Estudios Avanzados en Zonas Ãridas (CEAZA) “Programa Regional CONICYT R16A10003”. We thank SABIOD MI CNRS, EADM MaDICS CNRS and ANR-18-CE40-0014 SMILES supporting this research. We are grateful to colleagues at DCLDE 2018 and SOLAMAC 2018 conferences for useful comments on the preliminary version of this work. In this work we used only the free and open-source softwares Latex, Audacity and OCTAVE.
    Repository Name: Woods Hole Open Access Server
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  • 2
    Publication Date: 2022-05-25
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Ward, N. D., Megonigal, J. P., Bond-Lamberty, B., Bailey, V. L., Butman, D., Canuel, E. A., Diefenderfer, H., Ganju, N. K., Goni, M. A., Graham, E. B., Hopkinson, C. S., Khangaonkar, T., Langley, J. A., McDowell, N. G., Myers-Pigg, A. N., Neumann, R. B., Osburn, C. L., Price, R. M., Rowland, J., Sengupta, A., Simard, M., Thornton, P. E., Tzortziou, M., Vargas, R., Weisenhorn, P. B., & Windham-Myers, L. Representing the function and sensitivity of coastal interfaces in earth system models. Nature Communications, 11(1), (2020): 2458, doi:10.1038/s41467-020-16236-2.
    Description: Between the land and ocean, diverse coastal ecosystems transform, store, and transport material. Across these interfaces, the dynamic exchange of energy and matter is driven by hydrological and hydrodynamic processes such as river and groundwater discharge, tides, waves, and storms. These dynamics regulate ecosystem functions and Earth’s climate, yet global models lack representation of coastal processes and related feedbacks, impeding their predictions of coastal and global responses to change. Here, we assess existing coastal monitoring networks and regional models, existing challenges in these efforts, and recommend a path towards development of global models that more robustly reflect the coastal interface.
    Description: Funding for this work was provided by Pacific Northwest National Laboratory (PNNL) Laboratory Directed Research & Development (LDRD) as part of the Predicting Ecosystem Resilience through Multiscale Integrative Science (PREMIS) Initiative. PNNL is operated by Battelle for the U.S. Department of Energy under Contract DE-AC05-76RL01830. Additional support to J.P.M. was provided by the NSF-LTREB program (DEB-0950080, DEB-1457100, DEB-1557009), DOE-TES Program (DE-SC0008339), and the Smithsonian Institution. This manuscript was motivated by discussions held by co-authors during a three-day workshop at PNNL in Richland, WA: The System for Terrestrial Aquatic Research (STAR) Workshop: Terrestrial-Aquatic Research in Coastal Systems. The authors thank PNNL artist Nathan Johnson for preparing the figures in this manuscript and Terry Clark, Dr. Charlette Geffen, and Dr. Nancy Hess for their aid in organizing the STAR workshop. The authors thank all workshop participants not listed as authors for their valuable insight: Lihini Aluwihare (contributed to biogeochemistry discussions and development of concept for Fig. 3), Gautam Bisht (contributed to modeling discussion), Emmett Duffy (contributed to observational network discussions), Yilin Fang (contributed to modeling discussion), Jeremy Jones (contributed to biogeochemistry discussions), Roser Matamala (contributed to biogeochemistry discussions), James Morris (contributed to biogeochemistry discussions), Robert Twilley (contributed to biogeochemistry discussions), and Jesse Vance (contributed to observational network discussions). A full report on the workshop discussions can be found at https://www.pnnl.gov/publications/star-workshop-terrestrial-aquatic-research-coastal-systems.
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  • 3
    Publication Date: 2022-05-25
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Liang, Y., Lo, M., Lan, C., Seo, H., Ummenhofer, C. C., Yeager, S., Wu, R., & Steffen, J. D. Amplified seasonal cycle in hydroclimate over the Amazon river basin and its plume region. Nature Communications, 11(1), (2020): 4390, doi:10.1038/s41467-020-18187-0.
    Description: The Amazon river basin receives ~2000 mm of precipitation annually and contributes ~17% of global river freshwater input to the oceans; its hydroclimatic variations can exert profound impacts on the marine ecosystem in the Amazon plume region (APR) and have potential far-reaching influences on hydroclimate over the tropical Atlantic. Here, we show that an amplified seasonal cycle of Amazonia precipitation, represented by the annual difference between maximum and minimum values, during the period 1979–2018, leads to enhanced seasonalities in both Amazon river discharge and APR ocean salinity. An atmospheric moisture budget analysis shows that these enhanced seasonal cycles are associated with similar amplifications in the atmospheric vertical and horizontal moisture advections. Hierarchical sensitivity experiments using global climate models quantify the relationships of these enhanced seasonalities. The results suggest that an intensified hydroclimatological cycle may develop in the Amazonia atmosphere-land-ocean coupled system, favouring more extreme terrestrial and marine conditions.
    Description: M.-H.L., C.-W.L., and R.-J.W. are supported by the Ministry of Science and Technology in Taiwan under grant 106-2111-M-002-010-MY4. H.S. and J.D.S. are grateful for support from NOAA NA19OAR4310376 and NA17OAR4310255. C.C.U. acknowledges support from the U.S. National Science Foundation under grant OCE-1663704. The National Center for Atmospheric Research (NCAR) is a major facility sponsored by the US National Science Foundation (NSF) under Cooperative Agreement No. 1852977. We thank Dr. Young-Oh Kwon at Woods Hole Oceanographic Institution and Dr. Who Kim at NCAR for discussions about the ocean model experiment design. We thank Dr. Mehnaz Rashid at National Taiwan University and Wen-Yin Wu at the University of Texas at Austin in helping generate the high-resolution Amazon river mask. We also thank Dr. Gael Forget at Massachusetts Institue of Technology for comments on using ECCO and other ocean-state estimate products.
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  • 4
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Climate 33(9), (2020): 3845-3862, doi:10.1175/JCLI-D-19-0215.1.
    Description: The latitudinal structure of the Atlantic meridional overturning circulation (AMOC) variability in the North Atlantic is investigated using numerical results from three ocean circulation simulations over the past four to five decades. We show that AMOC variability south of the Labrador Sea (53°N) to 25°N can be decomposed into a latitudinally coherent component and a gyre-opposing component. The latitudinally coherent component contains both decadal and interannual variabilities. The coherent decadal AMOC variability originates in the subpolar region and is reflected by the zonal density gradient in that basin. It is further shown to be linked to persistent North Atlantic Oscillation (NAO) conditions in all three models. The interannual AMOC variability contained in the latitudinally coherent component is shown to be driven by westerlies in the transition region between the subpolar and the subtropical gyre (40°–50°N), through significant responses in Ekman transport. Finally, the gyre-opposing component principally varies on interannual time scales and responds to local wind variability related to the annual NAO. The contribution of these components to the total AMOC variability is latitude-dependent: 1) in the subpolar region, all models show that the latitudinally coherent component dominates AMOC variability on interannual to decadal time scales, with little contribution from the gyre-opposing component, and 2) in the subtropical region, the gyre-opposing component explains a majority of the interannual AMOC variability in two models, while in the other model, the contributions from the coherent and the gyre-opposing components are comparable. These results provide a quantitative decomposition of AMOC variability across latitudes and shed light on the linkage between different AMOC variability components and atmospheric forcing mechanisms.
    Description: The authors gratefully acknowledge support from the Physical Oceanography Program of the U.S. National Science Foundation (Awards OCE-1756143 and OCE-1537136) and the Climate Program Office of the National Oceanic and Atmospheric Administration (Award NA15OAR4310088). Gratitude is extended to Claus Böning and Arne Biastoch who shared ORCA025 output. S. Zou thanks F. Li, M. Buckley, and L. Li for helpful discussions. We also thank three anonymous reviewers for helpful suggestions.
    Keywords: Deep convection ; Ocean circulation ; Thermocline circulation
    Repository Name: Woods Hole Open Access Server
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  • 5
    Publication Date: 2022-05-25
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Gravinese, P. M., Page, H. N., Butler, C. B., Spadaro, A. J., Hewett, C., Considine, M., Lankes, D., & Fisher, S. Ocean acidification disrupts the orientation of postlarval Caribbean spiny lobsters. Scientific Reports, 10(1), (2020): 18092, doi:10.1038/s41598-020-75021-9.
    Description: Anthropogenic inputs into coastal ecosystems are causing more frequent environmental fluctuations and reducing seawater pH. One such ecosystem is Florida Bay, an important nursery for the Caribbean spiny lobster, Panulirus argus. Although adult crustaceans are often resilient to reduced seawater pH, earlier ontogenetic stages can be physiologically limited in their tolerance to ocean acidification on shorter time scales. We used a Y-maze chamber to test whether reduced-pH seawater altered the orientation of spiny lobster pueruli toward chemical cues produced by Laurencia spp. macroalgae, a known settlement cue for the species. We tested the hypothesis that pueruli conditioned in reduced-pH seawater would be less responsive to Laurencia spp. chemical cues than pueruli in ambient-pH seawater by comparing the proportion of individuals that moved to the cue side of the chamber with the proportion that moved to the side with no cue. We also recorded the amount of time (sec) before a response was observed. Pueruli conditioned in reduced-pH seawater were less responsive and failed to select the Laurencia cue. Our results suggest that episodic acidification of coastal waters might limit the ability of pueruli to locate settlement habitats, increasing postsettlement mortality.
    Description: We thank the Steinwachs Family Foundation, which provided funding that supported Gravinese’s postdoctoral fellowship at Mote Marine Laboratory and Aquarium. We also acknowledge the partial support provided by the St. Petersburg College Titan Achievement minigrant program. Page was supported by a Mote Marine Laboratory and Aquarium Postdoctoral Research Fellowship. Postlarval spiny lobsters were collected with a state-issued Special Activity License (SAL-17-1868G-SR). We also thank those who helped with animal collection throughout this work including in-kind support provided by E. Muller and the Mote CAOS facility, as well as E. Bartels and C. Walter of the Coral Reef Monitoring and Assessment Program at Mote Marine Laboratory and Aquarium, as well as other field personnel including: L. Toth, S. Perry, T. Parker, A. Fine, L. Humphrey, and many undergraduate interns. We also thank L. Toth, E. Ross, B. Sharp, C. Crowley, J. Butler, and B. Crowder for editorial comments.
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  • 6
    Publication Date: 2022-05-25
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Huang, J., Pickart, R. S., Huang, R. X., Lin, P., Brakstad, A., & Xu, F. Sources and upstream pathways of the densest overflow water in the Nordic Seas. Nature Communications, 11(1), (2020): 5389, doi:10.1038/s41467-020-19050-y.
    Description: Overflow water from the Nordic Seas comprises the deepest limb of the Atlantic Meridional Overturning Circulation, yet questions remain as to where it is ventilated and how it reaches the Greenland-Scotland Ridge. Here we use historical hydrographic data from 2005-2015, together with satellite altimeter data, to elucidate the source regions of the Denmark Strait and Faroe Bank Channel overflows and the pathways feeding these respective sills. A recently-developed metric is used to calculate how similar two water parcels are, based on potential density and potential spicity. This reveals that the interior of the Greenland Sea gyre is the primary wintertime source of the densest portion of both overflows. After subducting, the water progresses southward along several ridge systems towards the Greenland-Scotland Ridge. Kinematic evidence supports the inferred pathways. Extending the calculation back to the 1980s reveals that the ventilation occurred previously along the periphery of the Greenland Sea gyre.
    Description: Funding for the study was provided by the US National Science Foundation under grants OCE-1558742 (J.H., R.P.) and OCE-1259618 (P.L.); the Bergen Research Foundation under grant BFS2016REK01 (A.B.); and the National Natural Science Foundation of China No. 41576018 (F.X.) and 41606020 (F.X.).
    Repository Name: Woods Hole Open Access Server
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  • 7
    Publication Date: 2022-05-25
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Colin, S. P., Costello, J. H., Sutherland, K. R., Gemmell, B. J., Dabiri, J. O., & Du Clos, K. T. The role of suction thrust in the metachronal paddles of swimming invertebrates. Scientific Reports, 10(1), (2020): 17790, doi:10.1038/s41598-020-74745-y.
    Description: An abundance of swimming animals have converged upon a common swimming strategy using multiple propulsors coordinated as metachronal waves. The shared kinematics suggest that even morphologically and systematically diverse animals use similar fluid dynamic relationships to generate swimming thrust. We quantified the kinematics and hydrodynamics of a diverse group of small swimming animals who use multiple propulsors, e.g. limbs or ctenes, which move with antiplectic metachronal waves to generate thrust. Here we show that even at these relatively small scales the bending movements of limbs and ctenes conform to the patterns observed for much larger swimming animals. We show that, like other swimming animals, the propulsors of these metachronal swimmers rely on generating negative pressure along their surfaces to generate forward thrust (i.e., suction thrust). Relying on negative pressure, as opposed to high pushing pressure, facilitates metachronal waves and enables these swimmers to exploit readily produced hydrodynamic structures. Understanding the role of negative pressure fields in metachronal swimmers may provide clues about the hydrodynamic traits shared by swimming and flying animals.
    Description: This work was funded by National Science Foundation (NSF OCE 1829913 to SPC), the Alfred P. Sloan Foundation (to BJG) and the Gordon and Betty Moore Foundation (8835 to KRS). The work was also supported by the Roger Williams Foundation to Promote Scholarship and Teaching.
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  • 8
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Hahn, L. C., Storelvmo, T., Hofer, S., Parfitt, R., & Ummenhofer, C. C. Importance of Orography for Greenland cloud and melt response to atmospheric blocking. Journal of Climate, 33(10), (2020): 4187-4206, doi:10.1175/JCLI-D-19-0527.1.
    Description: More frequent high pressure conditions associated with atmospheric blocking episodes over Greenland in recent decades have been suggested to enhance melt through large-scale subsidence and cloud dissipation, which allows more solar radiation to reach the ice sheet surface. Here we investigate mechanisms linking high pressure circulation anomalies to Greenland cloud changes and resulting cloud radiative effects, with a focus on the previously neglected role of topography. Using reanalysis and satellite data in addition to a regional climate model, we show that anticyclonic circulation anomalies over Greenland during recent extreme blocking summers produce cloud changes dependent on orographic lift and descent. The resulting increased cloud cover over northern Greenland promotes surface longwave warming, while reduced cloud cover in southern and marginal Greenland favors surface shortwave warming. Comparison with an idealized model simulation with flattened topography reveals that orographic effects were necessary to produce area-averaged decreasing cloud cover since the mid-1990s and the extreme melt observed in the summer of 2012. This demonstrates a key role for Greenland topography in mediating the cloud and melt response to large-scale circulation variability. These results suggest that future melt will depend on the pattern of circulation anomalies as well as the shape of the Greenland Ice Sheet.
    Description: This research was supported by the Woods Hole Oceanographic Institution Summer Student Fellow program, by the U.S. National Science Foundation under AGS-1355339 to C.C.U., and by the European Research Council through Grant 758005.
    Keywords: Ice sheets ; Blocking ; Cloud cover ; Topographic effects ; Climate change ; Climate variability
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  • 9
    Publication Date: 2022-05-25
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Wiechmann, A. F., Martin, T. A., & Horb, M. E. CRISPR/Cas9 mediated mutation of the mtnr1a melatonin receptor gene causes rod photoreceptor degeneration in developing Xenopus tropicalis. Scientific Reports, 10(1), (2020): 13757, doi:10.1038/s41598-020-70735-2.
    Description: Nighttime surges in melatonin levels activate melatonin receptors, which synchronize cellular activities with the natural light/dark cycle. Melatonin receptors are expressed in several cell types in the retina, including the photon-sensitive rods and cones. Previous studies suggest that long-term photoreceptor survival and retinal health is in part reliant on melatonin orchestration of circadian homeostatic activities. This scenario would accordingly envisage that disruption of melatonin receptor signaling is detrimental to photoreceptor health. Using in vivo CRISPR/Cas9 genomic editing, we discovered that a small deletion mutation of the Mel1a melatonin receptor (mtnr1a) gene causes a loss of rod photoreceptors in retinas of developing Xenopus tropicalis heterozygous, but not homozygous mutant tadpoles. Cones were relatively spared from degeneration, and the rod loss phenotype was not obvious after metamorphosis. Localization of Mel1a receptor protein appeared to be about the same in wild type and mutant retinas, suggesting that the mutant protein is expressed at some level in mutant retinal cells. The severe impact on early rod photoreceptor viability may signify a previously underestimated critical role in circadian influences on long-term retinal health and preservation of sight. These data offer evidence that disturbance of homeostatic, circadian signaling, conveyed through a mutated melatonin receptor, is incompatible with rod photoreceptor survival.
    Description: The National Xenopus Resource (NXR) Genome Editing Workshop conducted at the Marine Biological Laboratory (MBL) contributed to the early development of this project (A.F.W. & M.E.H). We thank Dr. Marcin Wlizla, Sean McNamara, Rosie Falco, and Dr. Will Ratzen of the NXR and MBL for their advice and assistance with the F0 founders. We thank Cynthia Bulmer of the NIH Diabetes CoBRE (P20GM104934) Core Histology Facility at the University of Oklahoma Health Sciences Center (OUHSC) for preparing the histology specimens. We thank Dr. David Sherry of OUHSC for critically reading the initial version of the manuscript and his helpful advice during this study.
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  • 10
    Publication Date: 2022-05-25
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Dzwonkowski, B., Coogan, J., Fournier, S., Lockridge, G., Park, K., & Lee, T. Compounding impact of severe weather events fuels marine heatwave in the coastal ocean. Nature Communications, 11(1), (2020): 4623, doi:10.1038/s41467-020-18339-2.
    Description: Exposure to extreme events is a major concern in coastal regions where growing human populations and stressed natural ecosystems are at significant risk to such phenomena. However, the complex sequence of processes that transform an event from notable to extreme can be challenging to identify and hence, limit forecast abilities. Here, we show an extreme heat content event (i.e., a marine heatwave) in coastal waters of the northern Gulf of Mexico resulted from compounding effects of a tropical storm followed by an atmospheric heatwave. This newly identified process of generating extreme ocean temperatures occurred prior to landfall of Hurricane Michael during October of 2018 and, as critical contributor to storm intensity, likely contributed to the subsequent extreme hurricane. This pattern of compounding processes will also exacerbate other environmental problems in temperature-sensitive ecosystems (e.g., coral bleaching, hypoxia) and is expected to have expanding impacts under global warming predictions.
    Description: This work would not have been possible without the help of the Tech Support Group at the Dauphin Island Sea Lab. A portion of this work was conducted at the Jet Propulsion Laboratory, California Institute of Technology, under contract with NASA. This research was made possible by the NOAA RESTORE Science Program (NA17NOS4510101 and NA19NOS4510194) and NOAA NGI NMFS Regional Collaboration Network (18-NGI3-61).
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  • 11
    Publication Date: 2022-03-01
    Description: To examine the atmospheric responses to Arctic sea ice variability in the Northern Hemisphere cold season (from October to the following March), this study uses a coordinated set of large-ensemble experiments of nine atmospheric general circulation models (AGCMs) forced with observed daily varying sea ice, sea surface temperature, and radiative forcings prescribed during the 1979–2014 period, together with a parallel set of experiments where Arctic sea ice is substituted by its climatology. The simulations of the former set reproduce the near-surface temperature trends in reanalysis data, with similar amplitude, and their multimodel ensemble mean (MMEM) shows decreasing sea level pressure over much of the polar cap and Eurasia in boreal autumn. The MMEM difference between the two experiments allows isolating the effects of Arctic sea ice loss, which explain a large portion of the Arctic warming trends in the lower troposphere and drive a small but statistically significant weakening of the wintertime Arctic Oscillation. The observed interannual covariability between sea ice extent in the Barents–Kara Seas and lagged atmospheric circulation is distinguished from the effects of confounding factors based on multiple regression, and quantitatively compared to the covariability in MMEMs. The interannual sea ice decline followed by a negative North Atlantic Oscillation–like anomaly found in observations is also seen in the MMEM differences, with consistent spatial structure but much smaller amplitude. This result suggests that the sea ice impacts on trends and interannual atmospheric variability simulated by AGCMs could be underestimated, but caution is needed because internal atmospheric variability may have affected the observed relationship.
    Description: Published
    Description: 8419–8443
    Description: 2A. Fisica dell'alta atmosfera
    Description: JCR Journal
    Keywords: Arctic ; Sea ice ; Atmospheric circulation ; Climate models ; 01.01. Atmosphere
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 12
    Publication Date: 2022-02-14
    Description: The influence of the Atlantic multidecadal variability (AMV) on the North Atlantic storm track and eddy-driven jet in the winter season is assessed via a coordinated analysis of idealized simulations with state-of-the-art coupled models. Data used are obtained from a multimodel ensemble of AMV± experiments conducted in the framework of the Decadal Climate Prediction Project component C. These experiments are performed by nudging the surface of the Atlantic Ocean to states defined by the superimposition of observed AMV± anomalies onto the model climatology. A robust extratropical response is found in the form of a wave train extending from the Pacific to the Nordic seas. In the warm phase of the AMV compared to the cold phase, the Atlantic storm track is typically contracted and less extended poleward and the low-level jet is shifted toward the equator in the eastern Atlantic. Despite some robust features, the picture of an uncertain and model-dependent response of the Atlantic jet emerges and we demonstrate a link between model bias and the character of the jet response.
    Description: Published
    Description: 347-360
    Description: 4A. Oceanografia e clima
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 13
    Publication Date: 2022-06-20
    Description: Between 2003-2016, the Greenland ice sheet (GrIS) was one of the largest contributors to sea level rise, as it lost about 255 Gt of ice per year. This mass loss slowed in 2017 and 2018 to about 100 Gt yr−1. Here we examine further changes in rate of GrIS mass loss, by analyzing data from the GRACE-FO (Gravity Recovery and Climate Experiment – Follow On) satellite mission, launched in May 2018. Using simulations with regional climate models we show that the mass losses observed in 2017 and 2018 by the GRACE and GRACE-FO missions are lower than in any other two year period between 2003 and 2019, the combined period of the two missions. We find that this reduced ice loss results from two anomalous cold summers in western Greenland, compounded by snow-rich autumn and winter conditions in the east. For 2019, GRACE-FO reveals a return to high melt rates leading to a mass loss of 223 ± 12 Gt month−1 during the month of July alone, and a record annual mass loss of 532 ± 58 Gt yr−1.
    Repository Name: EPIC Alfred Wegener Institut
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  • 14
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    Nature Publishing Group
    In:  EPIC3Nature Climate Change, Nature Publishing Group, 12(3), pp. 249-255
    Publication Date: 2022-06-20
    Repository Name: EPIC Alfred Wegener Institut
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  • 15
    Publication Date: 2022-06-21
    Description: Author Posting. © American Meteorological Society, 2022. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 52(6),(2022): 1191-1204, https://doi.org/10.1175/jpo-d-21-0242.1.
    Description: A simplified quasigeostrophic (QG) analytical model together with an idealized numerical model are used to study the effect of uneven ice–ocean stress on the temporal evolution of the geostrophic current under sea ice. The tendency of the geostrophic velocity in the QG model is given as a function of the lateral gradient of vertical velocity and is further related to the ice–ocean stress with consideration of a surface boundary layer. Combining the analytical and numerical solutions, we demonstrate that the uneven stress between the ice and an initially surface-intensified, laterally sheared geostrophic current can drive an overturning circulation to trigger the displacement of isopycnals and modify the vertical structure of the geostrophic velocity. When the near-surface isopycnals become tilted in the opposite direction to the deeper ones, a subsurface velocity core is generated (via geostrophic setup). This mechanism should help understand the formation of subsurface currents in the edge of Chukchi and Beaufort Seas seen in observations. Furthermore, our solutions reveal a reversed flow extending from the bottom to the middepth, suggesting that the ice-induced overturning circulation potentially influences the currents in the deep layers of the Arctic Ocean, such as the Atlantic Water boundary current.
    Description: This work was funded by the National Key Research and Development Program of China (Grant 2017YFA0604600), the National Natural Science Foundation of China (Grant 41676019), the Fundamental Research Funds for the Central Universities (Grant 2019B81214), the Postgraduate Research and Practice Innovation Program of Jiangsu Province (Grant KYCX19_0384), and the National Science Foundation (MAS, Grants OPP-1822334, OCE-2122633).
    Keywords: Arctic ; Sea ice ; Channel flows ; Vertical motion ; Ekman pumping ; Idealized models ; Quasigeostrophic models
    Repository Name: Woods Hole Open Access Server
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  • 16
    Publication Date: 2022-05-26
    Description: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Renfrew, I. A., Pickart, R. S., Vage, K., Moore, G. W. K., Bracegirdle, T. J., Elvidge, A. D., Jeansson, E., Lachlan-Cope, T., McRaven, L. T., Papritz, L., Reuder, J., Sodemann, H., Terpstra, A., Waterman, S., Valdimarsson, H., Weiss, A., Almansi, M., Bahr, F., Brakstad, A., Barrell, C., Brooke, J. K., Brooks, B. J., Brooks, I. M., Brooks, M. E., Bruvik, E. M., Duscha, C., Fer, I., Golid, H. M., Hallerstig, M., Hessevik, I., Huang, J., Houghton, L., Jonsson, S., Jonassen, M., Jackson, K., Kvalsund, K., Kolstad, E. W., Konstali, K., Kristiansen, J., Ladkin, R., Lin, P., Macrander, A., Mitchell, A., Olafsson, H., Pacini, A., Payne, C., Palmason, B., Perez-Hernandez, M. D., Peterson, A. K., Petersen, G. N., Pisareva, M. N., Pope, J. O., Seidl, A., Semper, S., Sergeev, D., Skjelsvik, S., Soiland, H., Smith, D., Spall, M. A., Spengler, T., Touzeau, A., Tupper, G., Weng, Y., Williams, K. D., Yang, X., & Zhou, S. The Iceland Greenland Seas Project. Bulletin of the American Meteorological Society, 100(9), (2019): 1795-1817, doi:10.1175/BAMS-D-18-0217.1.
    Description: The Iceland Greenland Seas Project (IGP) is a coordinated atmosphere–ocean research program investigating climate processes in the source region of the densest waters of the Atlantic meridional overturning circulation. During February and March 2018, a field campaign was executed over the Iceland and southern Greenland Seas that utilized a range of observing platforms to investigate critical processes in the region, including a research vessel, a research aircraft, moorings, sea gliders, floats, and a meteorological buoy. A remarkable feature of the field campaign was the highly coordinated deployment of the observing platforms, whereby the research vessel and aircraft tracks were planned in concert to allow simultaneous sampling of the atmosphere, the ocean, and their interactions. This joint planning was supported by tailor-made convection-permitting weather forecasts and novel diagnostics from an ensemble prediction system. The scientific aims of the IGP are to characterize the atmospheric forcing and the ocean response of coupled processes; in particular, cold-air outbreaks in the vicinity of the marginal ice zone and their triggering of oceanic heat loss, and the role of freshwater in the generation of dense water masses. The campaign observed the life cycle of a long-lasting cold-air outbreak over the Iceland Sea and the development of a cold-air outbreak over the Greenland Sea. Repeated profiling revealed the immediate impact on the ocean, while a comprehensive hydrographic survey provided a rare picture of these subpolar seas in winter. A joint atmosphere–ocean approach is also being used in the analysis phase, with coupled observational analysis and coordinated numerical modeling activities underway.
    Description: The IGP has received funding from the U.S. National Science Foundation: Grant OCE-1558742; the U.K.’s Natural Environment Research Council: AFIS (NE/N009754/1); the Research Council of Norway: MOCN (231647), VENTILATE (229791), SNOWPACE (262710) and FARLAB (245907); and the Bergen Research Foundation (BFS2016REK01). We thank all those involved in the field work associated with the IGP, particularly the officers and crew of the Alliance, and the operations staff of the aircraft campaign.
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  • 17
    Publication Date: 2022-05-26
    Description: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Weizman, E. N., Tannenbaum, M., Tarrant, A. M., Hakim, O., & Levy, O. Chromatin dynamics enable transcriptional rhythms in the cnidarian Nematostella vectensis. Plos Genetics, 15(11), (2019): e1008397, doi: 10.1371/journal.pgen.1008397.
    Description: In animals, circadian rhythms are driven by oscillations in transcription, translation, and proteasomal degradation of highly conserved genes, resulting in diel cycles in the expression of numerous clock-regulated genes. Transcription is largely regulated through the binding of transcription factors to cis-regulatory elements within accessible regions of the chromatin. Chromatin remodeling is linked to circadian regulation in mammals, but it is unknown whether cycles in chromatin accessibility are a general feature of clock-regulated genes throughout evolution. To assess this, we applied an ATAC-seq approach using Nematostella vectensis, grown under two separate light regimes (light:dark (LD) and constant darkness (DD)). Based on previously identified N. vectensis circadian genes, our results show the coupling of chromatin accessibility and circadian transcription rhythmicity under LD conditions. Out of 180 known circadian genes, we were able to list 139 gene promoters that were highly accessible compared to common promoters. Furthermore, under LD conditions, we identified 259 active enhancers as opposed to 333 active enhancers under DD conditions, with 171 enhancers shared between the two treatments. The development of a highly reproducible ATAC-seq protocol integrated with published RNA-seq and ChIP-seq databases revealed the enrichment of transcription factor binding sites (such as C/EBP, homeobox, and MYB), which have not been previously associated with circadian signaling in cnidarians. These results provide new insight into the regulation of cnidarian circadian machinery. Broadly speaking, this supports the notion that the association between chromatin remodeling and circadian regulation arose early in animal evolution as reflected in this non-bilaterian lineage.
    Description: The research leading for this paper was funded by the Moore Foundation (https://www.moore.org), “Unwinding the Circadian Clock in a Sea Anemone” (Grant #4598) to A.T and O.L. The founders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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  • 18
    Publication Date: 2022-05-26
    Description: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Giuffre, C., Hinow, P., Jiang, H., & Strickler, J. R. Oscillations in the near-field feeding current of a calanoid copepod are useful for particle sensing. Scientific Reports, 9(1), (2019): 17742, doi: 10.1038/s41598-019-54264-1.
    Description: Calanoid copepods are small crustaceans that constitute a major element of aquatic ecosystems. Key to their success is their feeding apparatus consisting of sensor-studded mouth appendages that are in constant motion. These appendages generate a feeding current to enhance the encounter probability with food items. Additionally, sensing enables the organism to determine the position and quality of food particles, and to alter the near-field flow to capture and manipulate the particles for ingestion or rejection. Here we observe a freely swimming copepod Leptodiaptomus sicilis in multiple perspectives together with suspended particles that allow us to analyse the flow field created by the animal. We observe a highly periodic motion of the mouth appendages that is mirrored in oscillations of nearby tracer particles. We propose that the phase shift between the fluid and the particle velocities is sufficient for mechanical detection of the particles entrained in the feeding current. Moreover, we propose that an immersed algal cell may benefit from the excitation by increased uptake of dissolved inorganic compounds.
    Description: We acknowledge funding from the Simons Foundation (grant #278436 to PH) during two visits of HJ to Milwaukee. HJ was also supported by NSF grant OCE-1559062. We thank Dr. Russell Cuhel (School of Freshwater Sciences, University of Wisconsin - Milwaukee) for collecting the animals from Lake Michigan and four unknown readers for valuable comments.
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  • 19
    Publication Date: 2022-05-26
    Description: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Barry, P. H., Nakagawa, M., Giovannelli, D., de Moor, J. M., Schrenk, M., Seltzer, A. M., Manini, E., Fattorini, D., di Carlo, M., Regoli, F., Fullerton, K., & Lloyd, K. G. Helium, inorganic and organic carbon isotopes of fluids and gases across the Costa Rica convergent margin. Scientific Data, 6(1), (2019): 284, doi: 10.1038/s41597-019-0302-4.
    Description: In 2017, fluid and gas samples were collected across the Costa Rican Arc. He and Ne isotopes, C isotopes as well as total organic and inorganic carbon concentrations were measured. The samples (n = 24) from 2017 are accompanied by (n = 17) samples collected in 2008, 2010 and 2012. He-isotopes ranged from arc-like (6.8 RA) to crustal (0.5 RA). Measured dissolved inorganic carbon (DIC) δ13CVPDB values varied from 3.55 to −21.57‰, with dissolved organic carbon (DOC) following the trends of DIC. Gas phase CO2 only occurs within ~20 km of the arc; δ13CVPDB values varied from −0.84 to −5.23‰. Onsite, pH, conductivity, temperature and dissolved oxygen (DO) were measured; pH ranged from 0.9–10.0, conductivity from 200–91,900 μS/cm, temperatures from 23–89 °C and DO from 2–84%. Data were used to develop a model which suggests that ~91 ± 4.0% of carbon released from the slab/mantle beneath the Costa Rican forearc is sequestered within the crust by calcite deposition with an additional 3.3 ± 1.3% incorporated into autotrophic biomass.
    Description: This work was principally supported by a grant (G-2016-7206) from the Alfred P. Sloan Foundation and the Deep Carbon Observatory to P.H.B. In addition, P.H.B. was supported by NSF grant 1144559 during a portion of this project. D.G. was supported by an NSF grant (MCB 15–17567) and an ELSI Origins Network (EON) research Fellowship, which is supported by a grant from the John Templeton Foundation. The opinions expressed in this publication are those of the authors and do not necessarily reflect the views of the John Templeton Foundation. DG was also partially supported a Deep Life Modeling and Visualization Fellowship, which is supported by the Deep Carbon Observatory. This work was further supported in party by JSPS KAKENHI grants (JP17K14412, JP17H06105, JP17H02989) awarded to M.N., NSF OCE-1431598 and NASA Exobiology NNX16AL59G awarded to K.G.L. J.M.d.M. gratefully acknowledges funding from Universidad Nacional Costa Rica, the World Bank, and the Costa Rican Ley Transitorio 8933 used to acquire a laser carbon isotope system in collaboration with R. Sánchez-Murillo and G. Esquivel-Hernandez. M.N. produced the most data. We thank Patricia Barcala Dominguez for assistance with figure illustration. We thank Bruce Deck, Marten Wahlen and Keith Blackmon for analytical assistance at SIO. Finally, we acknowledge D. Hummer, T. Lopez, C.A. Pratt, Y. Alpízar Segura, A. Battaglia, P. Beaudry, G. Bini, M. Cascante, G. d’Errico, K. Fullerton, E. Gazel, G. González, S. A. Halldórsson, K. Iacovino, T. Ilanko, J.T. Kulongoski, M. Martínez, H. Miller, S. Ono, S. Patwardhan, C.J. Ramírez, F. Smedile, S. Turner, C. Vetriani, M. Yücel, C.J. Ballentine, T.P. Fischer, and D.R. Hilton, who were instrumental in proposal writing, sample collection, sample analysis and data analysis.
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  • 20
    Publication Date: 2022-05-26
    Description: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Chaput, D. L., Fowler, A. J., Seo, O., Duhn, K., Hansel, C. M., & Santelli, C. M. Mn oxide formation by phototrophs: spatial and temporal patterns, with evidence of an enzymatic superoxide-mediated pathway. Scientific Reports, 9(1), (2019): 18244, doi: 10.1038/s41598-019-54403-8.
    Description: Manganese (Mn) oxide minerals influence the availability of organic carbon, nutrients and metals in the environment. Oxidation of Mn(II) to Mn(III/IV) oxides is largely promoted by the direct and indirect activity of microorganisms. Studies of biogenic Mn(II) oxidation have focused on bacteria and fungi, with phototrophic organisms (phototrophs) being generally overlooked. Here, we isolated phototrophs from Mn removal beds in Pennsylvania, USA, including fourteen Chlorophyta (green algae), three Bacillariophyta (diatoms) and one cyanobacterium, all of which consistently formed Mn(III/IV) oxides. Isolates produced cell-specific oxides (coating some cells but not others), diffuse biofilm oxides, and internal diatom-specific Mn-rich nodules. Phototrophic Mn(II) oxidation had been previously attributed to abiotic oxidation mediated by photosynthesis-driven pH increases, but we found a decoupling of Mn oxide formation and pH alteration in several cases. Furthermore, cell-free filtrates of some isolates produced Mn oxides at specific time points, but this activity was not induced by Mn(II). Manganese oxide formation in cell-free filtrates occurred via reaction with the oxygen radical superoxide produced by soluble extracellular proteins. Given the known widespread ability of phototrophs to produce superoxide, the contribution of phototrophs to Mn(II) oxidation in the environment may be greater and more nuanced than previously thought.
    Description: This work was funded by a Smithsonian Scholarly Studies grant to CMS, by a Smithsonian Postdoctoral Fellowship to DLC, by the National Science Foundation, grant number CBET-1336496, to CMH and CMS, and by MnDRIVE Environment at the University of Minnesota to CMS. We thank Margaret Dunn and Cliff Denholm, Stream Restoration Inc., for assistance and access to field sites, Carolyn Zeiner (WHOI/Harvard) for useful discussions and advice regarding ROS experiments, as well as Jeff Post, Tim Rose and Tim Gooding (Smithsonian NMNH) for assistance with the SEM/EDS work. Portions of the laboratory work were conducted in and with the support of the L.A.B. facilities at the National Museum of Natural History, Smithsonian Institution.
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  • 21
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2019. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 49(12), (2019): 3127-3143, doi: 10.1175/JPO-D-19-0011.1.
    Description: The Intermediate Western Boundary Current (IWBC) transports Antarctic Intermediate Water across the Vitória–Trindade Ridge (VTR), a seamount chain at ~20°S off Brazil. Recent studies suggest that the IWBC develops a strong cyclonic recirculation in Tubarão Bight, upstream of the VTR, with weak time dependency. We herein use new quasi-synoptic observations, data from the Argo array, and a regional numerical model to describe the structure and variability of the IWBC and to investigate its dynamics. Both shipboard acoustic Doppler current profiler (ADCP) data and trajectories of Argo floats confirm the existence of the IWBC recirculation, which is also captured by our Regional Oceanic Modeling System (ROMS) simulation. An “intermediate-layer” quasigeostrophic (QG) model indicates that the ROMS time-mean flow is a good proxy for the IWBC steady state, as revealed by largely parallel isolines of streamfunction ψ⎯ and potential vorticity Q⎯; a ψ⎯−Q⎯ scatter diagram also shows that the IWBC is potentially unstable. Further analysis of the ROMS simulation reveals that remotely generated, westward-propagating nonlinear eddies are the main source of variability in the region. These eddies enter the domain through the Tubarão Bight eastern edge and strongly interact with the IWBC. As they are advected downstream and negotiate the local topography, the eddies grow explosively through horizontal shear production.
    Description: We thank Frank O. Smith for copy editing and proofreading this manuscript. This study was financed in part by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—CAPES, Brazil—Finance Code 001 and by Projeto REMARSUL (Processo CAPES 88882.158621/2014-01), Projeto VT-Dyn (Processo FAPESP 2015/21729-4) and Projeto SUBMESO (Processo CNPq 442926/2015-4). Rocha was supported by a WHOI Postdoctoral Scholarship.
    Description: 2020-06-06
    Keywords: South Atlantic Ocean ; Instability ; Mesoscale processes ; Intermediate waters ; In situ oceanic observations ; Quasigeostrophic models
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  • 22
    Publication Date: 2022-05-26
    Description: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Planes, S., Allemand, D., Agostini, S., Banaigs, B., Boissin, E., Boss, E., Bourdin, G., Bowler, C., Douville, E., Flores, J. M., Forcioli, D., Furla, P., Galand, P. E., Ghiglione, J. F., Gilson, E., Lombard, F., Moulin, C., Pesant, S., Poulain, J., Reynaud, S., Romac, S., Sullivan, M. B., Sunagawa, S., Thomas, O. P., Trouble, R., de Vargas, C., Thurber, R. V., Voolstra, C. R., Wincker, P., Zoccola, D., the Tara Pacific Consortium. The Tara Pacific expedition-A pan-ecosystemic approach of the "-omics" complexity of coral reef holobionts across the Pacific Ocean. Plos Biology, 17(9),(2019): e3000483, doi: 10.1371/journal.pbio.3000483.
    Description: Coral reefs are the most diverse habitats in the marine realm. Their productivity, structural complexity, and biodiversity critically depend on ecosystem services provided by corals that are threatened because of climate change effects—in particular, ocean warming and acidification. The coral holobiont is composed of the coral animal host, endosymbiotic dinoflagellates, associated viruses, bacteria, and other microeukaryotes. In particular, the mandatory photosymbiosis with microalgae of the family Symbiodiniaceae and its consequences on the evolution, physiology, and stress resilience of the coral holobiont have yet to be fully elucidated. The functioning of the holobiont as a whole is largely unknown, although bacteria and viruses are presumed to play roles in metabolic interactions, immunity, and stress tolerance. In the context of climate change and anthropogenic threats on coral reef ecosystems, the Tara Pacific project aims to provide a baseline of the “-omics” complexity of the coral holobiont and its ecosystem across the Pacific Ocean and for various oceanographically distinct defined areas. Inspired by the previous Tara Oceans expeditions, the Tara Pacific expedition (2016–2018) has applied a pan-ecosystemic approach on coral reefs throughout the Pacific Ocean, drawing an east–west transect from Panama to Papua New Guinea and a south–north transect from Australia to Japan, sampling corals throughout 32 island systems with local replicates. Tara Pacific has developed and applied state-of-the-art technologies in very-high-throughput genetic sequencing and molecular analysis to reveal the entire microbial and chemical diversity as well as functional traits associated with coral holobionts, together with various measures on environmental forcing. This ambitious project aims at revealing a massive amount of novel biodiversity, shedding light on the complex links between genomes, transcriptomes, metabolomes, organisms, and ecosystem functions in coral reefs and providing a reference of the biological state of modern coral reefs in the Anthropocene.
    Description: We are keen to thank the commitment of the people and the following institutions for their financial and scientific support that made this singular expedition possible: CNRS, PSL, CSM, EPHE, Genoscope/CEA, Inserm, Université Cote d’Azur, ANR, agnès b., UNESCO-IOC, the Veolia Environment Foundation, Région Bretagne, Serge Ferrari, Billerudkorsnas, Amerisource Bergen Company, Lorient Agglomeration, Oceans by Disney, the Prince Albert II de Monaco Foundation, L’Oréal, Biotherm, France Collectivités, Kankyo Station, Fonds Français pour l’Environnement Mondial (FFEM), Etienne BOURGOIS, and the Tara Ocean Foundation teams and crew. Tara Pacific would not exist without the continuous support of the participating institutes. This study has been conducted using E.U. Copernicus Marine Service Information and Mercator Ocean products. We acknowledged funding from the Investissement d’avenir projects France Génomique (ANR-10-INBS-09) and OCEANOMICS (ANR-11-BTBR-0008). RVT was funded by a Dimensions of Biodiversity NSF grant (#1442306) for this work. SS is supported by the ETH Zurich and Helmut Horten Foundation. FL is supported by Sorbonne Université, Institut Universitaire de France, and the Fondation CA-PCA. Finally, we thank the ANR for funding the project CORALGENE, which will support the work the Tara Pacific program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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  • 23
    Publication Date: 2022-05-26
    Description: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Kwon, Y., Seo, H., Ummenhofer, C. C., & Joyce, T. M. Impact of multidecadal variability in Atlantic SST on winter atmospheric blocking. Journal of Climate, 33(3), (2020): 867-892, doi: 10.1175/JCLI-D-19-0324.1.
    Description: Recent studies have suggested that coherent multidecadal variability exists between North Atlantic atmospheric blocking frequency and the Atlantic multidecadal variability (AMV). However, the role of AMV in modulating blocking variability on multidecadal times scales is not fully understood. This study examines this issue primarily using the NOAA Twentieth Century Reanalysis for 1901–2010. The second mode of the empirical orthogonal function for winter (December–March) atmospheric blocking variability in the North Atlantic exhibits oppositely signed anomalies of blocking frequency over Greenland and the Azores. Furthermore, its principal component time series shows a dominant multidecadal variability lagging AMV by several years. Composite analyses show that this lag is due to the slow evolution of the AMV sea surface temperature (SST) anomalies, which is likely driven by the ocean circulation. Following the warm phase of AMV, the warm SST anomalies emerge in the western subpolar gyre over 3–7 years. The ocean–atmosphere interaction over these 3–7-yr periods is characterized by the damping of the warm SST anomalies by the surface heat flux anomalies, which in turn reduce the overall meridional gradient of the air temperature and thus weaken the meridional transient eddy heat flux in the lower troposphere. The anomalous transient eddy forcing then shifts the eddy-driven jet equatorward, resulting in enhanced Rossby wave breaking and blocking on the northern flank of the jet over Greenland. The opposite is true with the AMV cold phases but with much shorter lags, as the evolution of SST anomalies differs in the warm and cold phases.
    Description: We gratefully acknowledge support from the NSF Climate and Large-scale Dynamics Program (AGS-1355339) to Y-OK, HS, CCU, and TMJ, the NASA Physical Oceanography Program (NNX13AM59G) to Y-OK, HS, and TMJ, NOAA CPO Climate Variability and Predictability Program (NA13OAR4310139) and DOE CESD Regional and Global Model Analysis Program (DE-SC0019492) to Y-OK, and NSF Physical Oceanography Program (OCE-1419235) to HS. We are very grateful to the three anonymous reviewers and editor Dr. Mingfang Ting, for their thorough and insightful suggestions. The NOAA 20CR dataset was downloaded from the NOAA Earth System Research Laboratory Physical Science Division webpage (https://www.esrl.noaa.gov/psd/data/20thC_Rean/). Support for the 20CR Project version 2c dataset is provided by the U.S. Department of Energy, Office of Science Biological and Environmental Research (BER), and by the National Oceanic and Atmospheric Administration Climate Program Office. The HadISST dataset was downloaded from the U.K. Met Office Hadley Centre webpage (https://www.metoffice.gov.uk/hadobs/hadisst/). The ERA-20C dataset was downloaded from the ECMWF webpage (https://apps.ecmwf.int/datasets/data/era20c-daily/). The ERSST5 dataset was provided by the NOAA Earth System Research Laboratory Physical Science Division (https://www.esrl.noaa.gov/psd/data/gridded/data.noaa.ersst.v5.html).
    Keywords: North Atlantic Ocean ; Atmosphere-ocean interaction ; Blocking ; Climate variability ; Multidecadal variability ; North Atlantic Oscillation
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  • 24
    Publication Date: 2022-05-26
    Description: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Ciacci, C., Grimmelpont, M. V., Corsi, I., Bergami, E., Curzi, D., Burini, D., Bouchet, V. M. P., Ambrogini, P., Gobbi, P., Ujiié, Y., Ishitani, Y., Coccioni, R., Bernhard, J. M., & Frontalini, F. Nanoparticle-biological interactions in a marine benthic foraminifer. Scientific Reports, (91), (2019): 19441, doi:10.1038/s41598-019-56037-2.
    Description: The adverse effects of engineered nanomaterials (ENM) in marine environments have recently attracted great attention although their effects on marine benthic organisms such as foraminifera are still largely overlooked. Here we document the effects of three negatively charged ENM, different in size and composition, titanium dioxide (TiO2), polystyrene (PS) and silicon dioxide (SiO2), on a microbial eukaryote (the benthic foraminifera Ammonia parkinsoniana) using multiple approaches. This research clearly shows the presence, within the foraminiferal cytoplasm, of metallic (Ti) and organic (PS) ENM that promote physiological stress. Specifically, marked increases in the accumulation of neutral lipids and enhanced reactive oxygen species production occurred in ENM-treated specimens regardless of ENM type. This study indicates that ENM represent ecotoxicological risks for this microbial eukaryote and presents a new model for the neglected marine benthos by which to assess natural exposure scenarios.
    Description: The authors are very grateful to two anonymous reviewers for their thoughtful and valuable comments that have greatly improved our contribution. NP characterization by DLS was conducted at the facilities of the Department of Biotechnologies, Chemistry and Pharmacy of the University of Siena (Italy). The authors acknowledge Prof. Andrea M. Atrei for the support in DLS analysis. Margot V. Grimmelpont’s stay at Urbino University was supported by an ERASMUS + fellowship.
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  • 25
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 50(2), (2020): 415-437, doi:10.1175/JPO-D-19-0019.1.
    Description: Results are presented from two dye release experiments conducted in the seasonal thermocline of the Sargasso Sea, one in a region of low horizontal strain rate (~10−6 s−1), the second in a region of intermediate horizontal strain rate (~10−5 s−1). Both experiments lasted ~6 days, covering spatial scales of 1–10 and 1–50 km for the low and intermediate strain rate regimes, respectively. Diapycnal diffusivities estimated from the two experiments were κz = (2–5) × 10−6 m2 s−1, while isopycnal diffusivities were κH = (0.2–3) m2 s−1, with the range in κH being less a reflection of site-to-site variability, and more due to uncertainties in the background strain rate acting on the patch combined with uncertain time dependence. The Site I (low strain) experiment exhibited minimal stretching, elongating to approximately 10 km over 6 days while maintaining a width of ~5 km, and with a notable vertical tilt in the meridional direction. By contrast, the Site II (intermediate strain) experiment exhibited significant stretching, elongating to more than 50 km in length and advecting more than 150 km while still maintaining a width of order 3–5 km. Early surveys from both experiments showed patchy distributions indicative of small-scale stirring at scales of order a few hundred meters. Later surveys show relatively smooth, coherent distributions with only occasional patchiness, suggestive of a diffusive rather than stirring process at the scales of the now larger patches. Together the two experiments provide important clues as to the rates and underlying processes driving diapycnal and isopycnal mixing at these scales.
    Description: Results are presented from two dye release experiments conducted in the seasonal thermocline of the Sargasso Sea, one in a region of low horizontal strain rate (~10−6 s−1), the second in a region of intermediate horizontal strain rate (~10−5 s−1). Both experiments lasted ~6 days, covering spatial scales of 1–10 and 1–50 km for the low and intermediate strain rate regimes, respectively. Diapycnal diffusivities estimated from the two experiments were κz = (2–5) × 10−6 m2 s−1, while isopycnal diffusivities were κH = (0.2–3) m2 s−1, with the range in κH being less a reflection of site-to-site variability, and more due to uncertainties in the background strain rate acting on the patch combined with uncertain time dependence. The Site I (low strain) experiment exhibited minimal stretching, elongating to approximately 10 km over 6 days while maintaining a width of ~5 km, and with a notable vertical tilt in the meridional direction. By contrast, the Site II (intermediate strain) experiment exhibited significant stretching, elongating to more than 50 km in length and advecting more than 150 km while still maintaining a width of order 3–5 km. Early surveys from both experiments showed patchy distributions indicative of small-scale stirring at scales of order a few hundred meters. Later surveys show relatively smooth, coherent distributions with only occasional patchiness, suggestive of a diffusive rather than stirring process at the scales of the now larger patches. Together the two experiments provide important clues as to the rates and underlying processes driving diapycnal and isopycnal mixing at these scales.
    Description: 2020-08-06
    Keywords: Ocean ; Atlantic Ocean ; Diapycnal mixing ; Diffusion ; Dispersion ; Mixing
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  • 26
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of the Atmospheric and Oceanic Technology 37(5), (2020): 789-806, doi:10.1175/JTECH-D-18-0244.1.
    Description: Realistic ocean state prediction and its validation rely on the availability of high quality in situ observations. To detect data errors, adequate quality check procedures must be designed. This paper presents procedures that take advantage of the ever-growing observation databases that provide climatological knowledge of the ocean variability in the neighborhood of an observation location. Local validity intervals are used to estimate binarily whether the observed values are considered as good or erroneous. Whereas a classical approach estimates validity bounds from first- and second-order moments of the climatological parameter distribution, that is, mean and variance, this work proposes to infer them directly from minimum and maximum observed values. Such an approach avoids any assumption of the parameter distribution such as unimodality, symmetry around the mean, peakedness, or homogeneous distribution tail height relative to distribution peak. To reach adequate statistical robustness, an extensive manual quality control of the reference dataset is critical. Once the data have been quality checked, the local minima and maxima reference fields are derived and the method is compared with the classical mean/variance-based approach. Performance is assessed in terms of statistics of good and bad detections. It is shown that the present size of the reference datasets allows the parameter estimates to reach a satisfactory robustness level to always make the method more efficient than the classical one. As expected, insufficient robustness persists in areas with an especially low number of samples and high variability.
    Description: This study has been conducted using EU Copernicus Marine Service Information and was supported by the European Union within the EU Copernicus Marine Service In Situ phase-I and phase-II contracts led by Ifremer. The publication was also supported by SOERE CTDO2 in France. The Argo data were collected and made freely available by the International Argo Program and the national programs that contribute to it (see http://www.argo.ucsd.edu, http://argo.jcommops.org). The Argo Program is part of the Global Ocean Observing System (http://doi.org/10.17882/42182). The marine mammal data were collected and made freely available by the International MEOP Consortium and the national programs that contribute to it (see http://www.meop.net; https://doi.org/10.17882/45461). Aleix Gelabert and Dídac Costa were the skippers of the OPOO, sponsored by the Intergovernmental Oceanographic Commission (UNESCO) and Pharmaton. The BWR is a periodic oceanic race organized by the Fundació Navegació Oceànica de Barcelona (FNOB). Reviewer D. Briand provided some useful comments on the final version of the draft paper before submission.
    Description: 2020-11-04
    Keywords: Ocean ; Climatology ; Salinity ; Temperature ; Data quality control ; Oceanic variability
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  • 27
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 50(5), (2020): 1245-1263, doi:10.1175/JPO-D-19-0213.1.
    Description: We use laboratory experiments and theoretical modeling to investigate the surface expression of a subglacial discharge plume, as occurs at many fjords around Greenland. The experiments consider a fountain that is released vertically into a homogeneous fluid, adjacent either to a vertical or a sloping wall, that then spreads horizontally at the free surface before sinking back to the bottom. We present a model that separates the fountain into two separate regions: a vertical fountain and a horizontal, negatively buoyant jet. The model is compared to laboratory experiments that are conducted over a range of volume fluxes, density differences, and ambient fluid depths. It is shown that the nondimensionalized length, width, and aspect ratio of the surface expression are dependent on the Froude number, calculated at the start of the negatively buoyant jet. The model is applied to observations of the surface expression from a Greenland subglacial discharge plume. In the case where the discharge plume reaches the surface with negative buoyancy the model can be used to estimate the discharge properties at the base of the glacier.
    Description: We gratefully acknowledge technical assistance from Anders Jensen and thank anonymous reviewers for improving the clarity of the manuscript. CM thanks the Weston Howard Jr. Scholarship for funding. Support to CC was given by NSF project OCE-1434041 and OCE-1658079.
    Description: 2020-10-27
    Keywords: Ocean ; Glaciers ; Ice sheets ; Convection ; Laboratory/physical models
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  • 28
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Marzen, R. E., Shillington, D. J., Lizarralde, D., Knapp, J. H., Heffner, D. M., Davis, J. K., & Harder, S. H. Limited and localized magmatism in the Central Atlantic Magmatic Province. Nature Communications, 11(1), (2020): 3397, doi:10.1038/s41467-020-17193-6.
    Description: The Central Atlantic Magmatic Province (CAMP) is the most aerially extensive magmatic event in Earth’s history, but many questions remain about its origin, volume, and distribution. Despite many observations of CAMP magmatism near Earth’s surface, few constraints exist on CAMP intrusions at depth. Here we present detailed constraints on crustal and upper mantle structure from wide-angle seismic data across the Triassic South Georgia Rift that formed shortly before CAMP. Lower crustal magmatism is concentrated where synrift sedimentary fill is thickest and the crust is thinnest, suggesting that lithospheric thinning influenced the locus and volume of magmatism. The limited distribution of lower crustal intrusions implies modest total CAMP volumes of 85,000 to 169,000 km3 beneath the South Georgia Rift, consistent with moderately elevated mantle potential temperatures (〈1500 °C). These results suggest that CAMP magmatism in the South Georgia Rift is caused by syn-rift decompression melting of a warm, enriched mantle.
    Description: This project was funded by an NSF GRFP fellowship DGE 16-44869 and a grant from the National Science Foundation’s Division of Earth Sciences (NSF-EAR) EarthScope program through the collaborative awards EAR-1144534/−1144829/−1144391. Data collection was made possible with help from IRIS PASSCAL, the University of Texas El Paso Seismic Source Facility, the teams of students who deployed and recovered geophones, and the support of landowners and county and state officials. We thank Alistair Harding for the VMTomo code, Nathan Miller for the PyVM toolbox, and William Wilcock for maintaining the Upicker package to pick arrivals.
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  • 29
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 50(3), (2020): 679-694, doi:10.1175/JPO-D-19-0218.1.
    Description: The zonally integrated flow in a basin can be separated into the divergent/nondivergent parts, and a uniquely defined meridional overturning circulation (MOC) can be calculated. For a basin with significant volume exchange at zonal open boundaries, this method is competent in removing the components associated with the nonzero source terms due to zonal transports at open boundaries. This method was applied to the zonally integrated flow in the Indian Ocean basin extended all the way to the Antarctic by virtue of the ECCO dataset. The contributions due to two major zonal flow systems at open boundaries, the Indonesian Throughflow (ITF) and the Antarctic Circumpolar Current (ACC), were well separated from the rotational flow component, and a nondivergent overturning circulation pattern was identified. Comparisons with previous studies on the MOC of the Indian Ocean in different seasons showed overall consistency but with refinements in details to the south of the entry of the ITF, reflecting the influence of ITF on the MOC pattern in the domain. Other options of decomposition are also examined.
    Description: LH was supported by the National Basic Research Program of China through Grant 2019YFA0606703 and “The Fundamental Research Funds of Shandong University” (2019GN051). The authors thank the anonymous reviewers and the editor for their constructive comments. Code availability: The Matlab code that performs the decomposition and produces some figures in this paper is available at https://github.com/lei-han-SDU/IMOC/.
    Description: 2020-09-02
    Keywords: Meridional overturning circulation ; Ocean circulation ; Streamfunction
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  • 30
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Hopkinson, B. M., King, A. C., Owen, D. P., Johnson-Roberson, M., Long, M. H., & Bhandarkar, S. M. Automated classification of three-dimensional reconstructions of coral reefs using convolutional neural networks. PLoS One, 15(3), (2020): e0230671, doi: 10.1371/journal.pone.0230671.
    Description: Coral reefs are biologically diverse and structurally complex ecosystems, which have been severally affected by human actions. Consequently, there is a need for rapid ecological assessment of coral reefs, but current approaches require time consuming manual analysis, either during a dive survey or on images collected during a survey. Reef structural complexity is essential for ecological function but is challenging to measure and often relegated to simple metrics such as rugosity. Recent advances in computer vision and machine learning offer the potential to alleviate some of these limitations. We developed an approach to automatically classify 3D reconstructions of reef sections and assessed the accuracy of this approach. 3D reconstructions of reef sections were generated using commercial Structure-from-Motion software with images extracted from video surveys. To generate a 3D classified map, locations on the 3D reconstruction were mapped back into the original images to extract multiple views of the location. Several approaches were tested to merge information from multiple views of a point into a single classification, all of which used convolutional neural networks to classify or extract features from the images, but differ in the strategy employed for merging information. Approaches to merging information entailed voting, probability averaging, and a learned neural-network layer. All approaches performed similarly achieving overall classification accuracies of ~96% and 〉90% accuracy on most classes. With this high classification accuracy, these approaches are suitable for many ecological applications.
    Description: This study was funded by grants from the Alfred P. Sloan Foundation (BMH, BR2014-049; https://sloan.org), and the National Science Foundation (MHL, OCE-1657727; https://www.nsf.gov). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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  • 31
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Domeignoz-Horta, L. A., Pold, G., Liu, X. A., Frey, S. D., Melillo, J. M., & DeAngelis, K. M. Microbial diversity drives carbon use efficiency in a model soil. Nature Communications, 11(1), (2020): 3684, doi:10.1038/s41467-020-17502-z.
    Description: Empirical evidence for the response of soil carbon cycling to the combined effects of warming, drought and diversity loss is scarce. Microbial carbon use efficiency (CUE) plays a central role in regulating the flow of carbon through soil, yet how biotic and abiotic factors interact to drive it remains unclear. Here, we combine distinct community inocula (a biotic factor) with different temperature and moisture conditions (abiotic factors) to manipulate microbial diversity and community structure within a model soil. While community composition and diversity are the strongest predictors of CUE, abiotic factors modulated the relationship between diversity and CUE, with CUE being positively correlated with bacterial diversity only under high moisture. Altogether these results indicate that the diversity × ecosystem-function relationship can be impaired under non-favorable conditions in soils, and that to understand changes in soil C cycling we need to account for the multiple facets of global changes.
    Description: Funding for this project was provided by the Department of Energy grant DE-SC0016590 to K.M.D. and S.D.F., and an American Association of University Women Dissertation fellowship to G.P. We would also like to thank Stuart Grandy and Kevin Geyer for the fruitful discussions and Mary Waters, Courtney Bly and Ana Horta for their help with samples processing.
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  • 32
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Tao, C., Seyfried, W. E., Jr., Lowell, R. P., Liu, Y., Liang, J., Guo, Z., Ding, K., Zhang, H., Liu, J., Qiu, L., Egorov, I., Liao, S., Zhao, M., Zhou, J., Deng, X., Li, H., Wang, H., Cai, W., Zhang, G., Zhou, H., Lin, J., & Li, W. Deep high-temperature hydrothermal circulation in a detachment faulting system on the ultra-slow spreading ridge. Nature Communications, 11(1), (2020): 1300, doi:10.1038/s41467-020-15062-w.
    Description: Coupled magmatic and tectonic activity plays an important role in high-temperature hydrothermal circulation at mid-ocean ridges. The circulation patterns for such systems have been elucidated by microearthquakes and geochemical data over a broad spectrum of spreading rates, but such data have not been generally available for ultra-slow spreading ridges. Here we report new geophysical and fluid geochemical data for high-temperature active hydrothermal venting at Dragon Horn area (49.7°E) on the Southwest Indian Ridge. Twin detachment faults penetrating to the depth of 13 ± 2 km below the seafloor were identified based on the microearthquakes. The geochemical composition of the hydrothermal fluids suggests a long reaction path involving both mafic and ultramafic lithologies. Combined with numerical simulations, our results demonstrate that these hydrothermal fluids could circulate ~ 6 km deeper than the Moho boundary and to much greater depths than those at Trans-Atlantic Geotraverse and Logachev-1 hydrothermal fields on the Mid-Atlantic Ridge.
    Description: This work was supported by National Key R&D Program of China under contract no. 2018YFC0309901, 2017YFC0306603, 2017YFC0306803, and 2017YFC0306203, COMRA Major Project under contract No. DY135-S1-01-01 and No. DY135-S1-01-06.
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  • 33
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published inWeber, L., & Apprill, A. Diel, daily, and spatial variation of coral reef seawater microbial communities. PLoS One, 15(3), (2020): e0229442, doi: 10.1371/journal.pone.0229442.
    Description: Reef organisms influence microorganisms within the surrounding seawater, yet the spatial and temporal dynamics of seawater microbial communities located in proximity to corals are rarely investigated. To better understand reef seawater microbial community dynamics over time and space, we collected small-volume seawater samples during the day and night over a 72 hour period from three locations that differed in spatial distance from 5 Porites astreoides coral colonies on a shallow reef in St. John, U.S. Virgin Islands: near-coral (sampled 5 cm horizontally from each colony), reef-depth (sampled 2 m above each colony) and surface seawater (sampled 1 m from the seawater surface). At all time points and locations, we quantified abundances of microbial cells, sequenced small subunit rRNA genes of bacterial and archaeal communities, and measured inorganic nutrient concentrations. Prochlorococcus and Synechococcus cells were consistently elevated at night compared to day and these abundances changed over time, corresponding with temperature, nitrite, and silicate concentrations. During the day, bacterial and archaeal alpha diversity was significantly higher in reef-depth and near-coral seawater compared to the surface seawater, signifying that the reef influences the diversity of the seawater microorganisms. At night, alpha diversity decreased across all samples, suggesting that photosynthesis may favor a more taxonomically diverse community. While Prochlorococcus exhibited consistent temporal rhythmicity, additional taxa were enriched in reef seawater at night compared to day or in reef-depth compared to surface seawater based on their normalized sequence counts. There were some significant differences in nutrient concentrations and cell abundances between reef-depth and near-coral seawater but no clear trends. This study demonstrates that temporal variation supersedes small-scale spatial variation in proximity to corals in reef seawater microbial communities. As coral reefs continue to change in benthic composition worldwide, monitoring microbial composition in response to temporal changes and environmental fluctuations will help discern normal variability from longer lasting changes attributed to anthropogenic stressors and global climate change.
    Description: This work was supported by a National Science Foundation (NSF; https://www.nsf.gov/) Graduate Research Fellowship award to L.Weber. This research was also supported by NSF award OCE-1536782 to A. Mooney, J. Llopiz, and A. Apprill and NSF award OCE-1736288 to A. Apprill. Additionally, this work was supported by the NOAA Cooperative Institutes award NA19OAR4320074 to A.A. and E. Kujawinski and the Andrew W. Mellon Foundation Endowed Fund for Innovative Research to A.A.
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  • 34
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Keya, J. J., Kudoh, H., Kabir, A. M. R., Inoue, D., Miyamoto, N., Tani, T., Kakugo, A., & Shikinaka, K. Radial alignment of microtubules through tubulin polymerization in an evaporating droplet. Plos One, 15(4), (2020): e0231352, doi:10.1371/journal.pone.0231352.
    Description: We report the formation of spherulites from droplets of highly concentrated tubulin solution via nucleation and subsequent polymerization to microtubules (MTs) under water evaporation by heating. Radial alignment of MTs in the spherulites was confirmed by the optical properties of the spherulites observed using polarized optical microscopy and fluorescence microscopy. Temperature and concentration of tubulins were found as important parameters to control the spherulite pattern formation of MTs where evaporation plays a significant role. The alignment of MTs was regulated reversibly by temperature induced polymerization and depolymerization of tubulins. The formation of the MTs patterns was also confirmed at the molecular level from the small angle X-ray measurements. This work provides a simple method for obtaining radially aligned arrays of MTs.
    Description: Fund receiver: Akira Kakugo Grant-in-Aid for Scientific Research on Innovative Areas (Grant Nos. JP24104004 and 18H05423) and a Grant-in-Aid for Scientific Research (A) (Grant No. 18H03673) from kaken. NO - The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
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  • 35
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Bajnai, D., Guo, W., Spötl, C., Coplen, T. B., Methner, K., Löffler, N., Krsnik, E., Gischler, E., Hansen, M., Henkel, D., Price, G. D., Raddatz, J., Scholz, D., & Fiebig, J. Dual clumped isotope thermometry resolves kinetic biases in carbonate formation temperatures. Nature Communications, 11(1), (2020): 4005, doi:10.1038/s41467-020-17501-0.
    Description: Surface temperature is a fundamental parameter of Earth’s climate. Its evolution through time is commonly reconstructed using the oxygen isotope and the clumped isotope compositions of carbonate archives. However, reaction kinetics involved in the precipitation of carbonates can introduce inaccuracies in the derived temperatures. Here, we show that dual clumped isotope analyses, i.e., simultaneous ∆47 and ∆48 measurements on the single carbonate phase, can identify the origin and quantify the extent of these kinetic biases. Our results verify theoretical predictions and evidence that the isotopic disequilibrium commonly observed in speleothems and scleractinian coral skeletons is inherited from the dissolved inorganic carbon pool of their parent solutions. Further, we show that dual clumped isotope thermometry can achieve reliable palaeotemperature reconstructions, devoid of kinetic bias. Analysis of a belemnite rostrum implies that it precipitated near isotopic equilibrium and confirms the warmer-than-present temperatures during the Early Cretaceous at southern high latitudes.
    Description: This work became possible through DFG grant “INST 161/871-1” and the Investment in Science Fund at Woods Hole Oceanographic Institution. The authors would like to thank Sven Hofmann and Manuel Schumann for their assistance in the joint Goethe University – Senckenberg BiK-F Stable Isotope Facility at the Institute of Geosciences, Goethe University Frankfurt. K.M. acknowledges funding through “DFG ME 4955/1-1”, E.K. through “DFG MU 2845/6-1”, D.S. through “DFG SCHO 1274/8-1” and “DFG SCHO 1274/11-1”, and M.H. through “DFG HA 8694/1-1”. C.S. acknowledges funding from the University of Innsbruck. A review of the manuscript by David Evans on behalf of the USGS is acknowledged.
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  • 36
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Rodysill, J. R., Donnelly, J. P., Sullivan, R., Lane, P. D., Toomey, M., Woodruff, J. D., Hawkes, A. D., MacDonald, D., d'Entremont, N., McKeon, K., Wallace, E., & van Hengstum, P. J. Historically unprecedented Northern Gulf of Mexico hurricane activity from 650 to 1250 CE. Scientific Reports, 10(1), (2020): 19092. doi:10.1038/s41598-020-75874-0.
    Description: Hurricane Michael (2018) was the first Category 5 storm on record to make landfall on the Florida panhandle since at least 1851 CE (Common Era), and it resulted in the loss of 59 lives and $25 billion in damages across the southeastern U.S. This event placed a spotlight on recent intense (exceeding Category 4 or 5 on the Saffir-Simpson Hurricane Wind Scale) hurricane landfalls, prompting questions about the natural range in variability of hurricane activity that the instrumental record is too short to address. Of particular interest is determining whether the frequency of recent intense hurricane landfalls in the northern Gulf of Mexico (GOM) is within or outside the natural range of intense hurricane activity prior to 1851 CE. In this study, we identify intense hurricane landfalls in northwest Florida during the past 2000 years based on coarse anomaly event detection from two coastal lacustrine sediment archives. We identified a historically unprecedented period of heightened storm activity common to four Florida panhandle localities from 650 to 1250 CE and a shift to a relatively quiescent storm climate in the GOM spanning the past six centuries. Our study provides long-term context for events like Hurricane Michael and suggests that the observational period 1851 CE to present may underrepresent the natural range in landfalling hurricane activity.
    Description: Funding for this project was provided by the Strategic Environmental Research and Development Program (SERDP) grant and NSF awards 0903020, 1902463, and 1854980 awarded to Jeffrey Donnelly, and the USGS Land Change Science Program.
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  • 37
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Kennedy, E. B. L., Buresch, K. C., Boinapally, P., & Hanlon, R. T. Octopus arms exhibit exceptional flexibility. Scientific Reports, 10(1), (2020): 20872. doi:10.1038/s41598-020-77873-7.
    Description: The octopus arm is often referred to as one of the most flexible limbs in nature, yet this assumption requires detailed inspection given that this has not been measured comprehensively for all portions of each arm. We investigated the diversity of arm deformations in Octopus bimaculoides with a frame-by-frame observational analysis of laboratory video footage in which animals were challenged with different tasks. Diverse movements in these hydrostatic arms are produced by some combination of four basic deformations: bending (orally, aborally; inward, outward), torsion (clockwise, counter-clockwise), elongation, and shortening. More than 16,500 arm deformations were observed in 120 min of video. Results showed that all eight arms were capable of all four types of deformation along their lengths and in all directions. Arms function primarily to bring the sucker-lined oral surface in contact with target surfaces. Bending was the most common deformation observed, although the proximal third of the arms performed relatively less bending and more shortening and elongation as compared with other arm regions. These findings demonstrate the exceptional flexibility of the octopus arm and provide a basis for investigating motor control of the entire arm, which may aid the future development of soft robotics.
    Description: We gratefully acknowledge funding from Grant N00014-19-1-2445 from the Office of Naval Research, Tom McKenna and Marc Steinberg, Program Managers. We also thank the staff of the Marine Resources Center at MBL for assistance with water quality measurements, seawater system maintenance, and collection of food items for octopuses.
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  • 38
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Naert, T., Tulkens, D., Edwards, N. A., Carron, M., Shaidani, N. I., Wlizla, M., Boel, A., Demuynck, S., Horb, M. E., Coucke, P., Willaert, A., Zorn, A. M., & Vleminckx, K. Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos. Scientific Reports, 10(1), (2020): 14662, doi:10.1038/s41598-020-71412-0.
    Description: CRISPR/Cas9 genome editing has revolutionized functional genomics in vertebrates. However, CRISPR/Cas9 edited F0 animals too often demonstrate variable phenotypic penetrance due to the mosaic nature of editing outcomes after double strand break (DSB) repair. Even with high efficiency levels of genome editing, phenotypes may be obscured by proportional presence of in-frame mutations that still produce functional protein. Recently, studies in cell culture systems have shown that the nature of CRISPR/Cas9-mediated mutations can be dependent on local sequence context and can be predicted by computational methods. Here, we demonstrate that similar approaches can be used to forecast CRISPR/Cas9 gene editing outcomes in Xenopus tropicalis, Xenopus laevis, and zebrafish. We show that a publicly available neural network previously trained in mouse embryonic stem cell cultures (InDelphi-mESC) is able to accurately predict CRISPR/Cas9 gene editing outcomes in early vertebrate embryos. Our observations can have direct implications for experiment design, allowing the selection of guide RNAs with predicted repair outcome signatures enriched towards frameshift mutations, allowing maximization of CRISPR/Cas9 phenotype penetrance in the F0 generation.
    Description: Research in the Vleminckx laboratory is supported by the Research Foundation—Flanders (FWO-Vlaanderen) (Grants G0A1515N and G029413N), by the Belgian Science Policy (Interuniversity Attraction Poles—IAP7/07) and by the Concerted Research Actions from Ghent University (BOF15/GOA/011). Further support was obtained by the Hercules Foundation, Flanders (Grant AUGE/11/14) and the Desmoid Tumor Research Foundation and the Desmoid Tumour Foundation Canada. T.N. is funded by “Kom op tegen Kanker” (Stand up to Cancer), the Flemish cancer society and previously held PhD fellowship with VLAIO-HERMES during the course of this work. D.T. and M. C. hold a PhD fellowship from the Research Foundation-Flanders (FWO-Vlaanderen). The Zorn Lab is supported by Funding from NIH National Institute of Child Health and Human Development (NICHD) P01 HD093363. A.W. and A.B. are supported by the Ghent University (Universiteit Gent) Methusalem grant BOFMET2015000401 to Anne De Paepe. The National Xenopus Resource and Horb lab is supported by funding from the National Institutes of Health (P40 OD010997 and R01 HD084409).
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  • 39
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    American Meteorological Society
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 50(9), (2020): 2491-2506, doi:10.1175/JPO-D-20-0056.1.
    Description: An idealized two-layer shallow water model is applied to the study of the dynamics of the Arctic Ocean halocline. The model is forced by a surface stress distribution reflective of the observed wind stress pattern and ice motion and by an inflow representing the flow of Pacific Water through Bering Strait. The model reproduces the main elements of the halocline circulation: an anticyclonic Beaufort Gyre in the western basin (representing the Canada Basin), a cyclonic circulation in the eastern basin (representing the Eurasian Basin), and a Transpolar Drift between the two gyres directed from the upwind side of the basin to the downwind side of the basin. Analysis of the potential vorticity budget shows a basin-averaged balance primarily between potential vorticity input at the surface and dissipation at the lateral boundaries. However, advection is a leading-order term not only within the anticyclonic and cyclonic gyres but also between the gyres. This means that the eastern and western basins are dynamically connected through the advection of potential vorticity. Both eddy and mean fluxes play a role in connecting the regions of potential vorticity input at the surface with the opposite gyre and with the viscous boundary layers. These conclusions are based on a series of model runs in which forcing, topography, straits, and the Coriolis parameter were varied.
    Description: This study was supported by National Science Foundation Grant OPP-1822334. Comments and suggestions from two anonymous referees greatly helped to improve the paper.
    Description: 2021-02-17
    Keywords: Eddies ; Ekman pumping/transport ; Ocean circulation ; Ocean dynamics ; Potential vorticity ; Shallow-water equations
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  • 40
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Boissin, E., Neglia, V., Baksay, S., Micu, D., Bat, L., Topaloglu, B., Todorova, V., Panayotova, M., Kruschel, C., Milchakova, N., Voutsinas, E., Beqiraj, S., Nasto, I., Aglieri, G., Taviani, M., Zane, L., & Planes, S. Chaotic genetic structure and past demographic expansion of the invasive gastropod Tritia neritea in its native range, the Mediterranean Sea. Scientific Reports, 10(1), (2020): 21624. doi:10.1038/s41598-020-77742-3.
    Description: To better predict population evolution of invasive species in introduced areas it is critical to identify and understand the mechanisms driving genetic diversity and structure in their native range. Here, we combined analyses of the mitochondrial COI gene and 11 microsatellite markers to investigate both past demographic history and contemporaneous genetic structure in the native area of the gastropod Tritia neritea, using Bayesian skyline plots (BSP), multivariate analyses and Bayesian clustering. The BSP framework revealed population expansions, dated after the last glacial maximum. The haplotype network revealed a strong geographic clustering. Multivariate analyses and Bayesian clustering highlighted the strong genetic structure at all scales, between the Black Sea and the Adriatic Sea, but also within basins. Within basins, a random pattern of genetic patchiness was observed, suggesting a superimposition of processes involving natural biological effects (no larval phase and thus limited larval dispersal) and putative anthropogenic transport of specimens. Contrary to the introduced area, no isolation-by-distance patterns were recovered in the Mediterranean or the Black Seas, highlighting different mechanisms at play on both native and introduced areas, triggering unknown consequences for species’ evolutionary trajectories. These results of Tritia neritea populations on its native range highlight a mixture of ancient and recent processes, with the effects of paleoclimates and life history traits likely tangled with the effects of human-mediated dispersal.
    Description: This project was funded by the European FP7 CoCoNet project (Ocean.2011-4, grant agreement #287844) and we are grateful to the whole CoCoNET consortium. We are grateful to the following people for their critical help with logistics and field work ‘Antheus srl (Lecce, Italy)’; S Bevilacqua, G Guarnieri, S Fraschetti and T Terlizzi (University of Salento, Italy); L Angeletti and M Sigovini (ISMAR, Italy); D Shamrey (IBSS, Sevastopol); A Anastasopoulou, MA Pancucci-Papadopoulou and S Reizopoulou (HCMR, Greece) and E Hajdëri (Catholic University ‘Our Lady of Good Counsel’, Tirana). Thank you to J Almany for English corrections. This is ISMAR-CNR scientific contribution n1987. E Boissin was supported by a European Marie Curie postdoctoral fellowship MC-CIG-618480.
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  • 41
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Bulletin of the American Meteorological Society 101(6), (2020): E897-E904, doi:10.1175/BAMS-D-19-0047.1.
    Description: Over the past 15 years, numerous studies have suggested that the sinking branches of Earth’s Hadley circulation and the associated subtropical dry zones have shifted poleward over the late twentieth century and early twenty-first century. Early estimates of this tropical widening from satellite observations and reanalyses varied from 0.25° to 3° latitude per decade, while estimates from global climate models show widening at the lower end of the observed range. In 2016, two working groups, the U.S. Climate Variability and Predictability (CLIVAR) working group on the Changing Width of the Tropical Belt and the International Space Science Institute (ISSI) Tropical Width Diagnostics Intercomparison Project, were formed to synthesize current understanding of the magnitude, causes, and impacts of the recent tropical widening evident in observations. These working groups concluded that the large rates of observed tropical widening noted by earlier studies resulted from their use of metrics that poorly capture changes in the Hadley circulation, or from the use of reanalyses that contained spurious trends. Accounting for these issues reduces the range of observed expansion rates to 0.25°–0.5° latitude decade‒1—within the range from model simulations. Models indicate that most of the recent Northern Hemisphere tropical widening is consistent with natural variability, whereas increasing greenhouse gases and decreasing stratospheric ozone likely played an important role in Southern Hemisphere widening. Whatever the cause or rate of expansion, understanding the regional impacts of tropical widening requires additional work, as different forcings can produce different regional patterns of widening.
    Description: We thank U.S. CLIVAR and ISSI for funding the two working groups. We thank all members of the working groups for helpful discussions, and the U.S. CLIVAR and ISSI offices and their sponsoring agencies (NASA, NOAA, NSF, DOE, ESA, Swiss Confederation, Swiss Academy of Sciences, and University of Bern) for supporting these groups and activities.
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  • 42
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Liu, D., Ma, Q., Valiela, I., Anderson, D. M., Keesing, J. K., Gao, K., Zhen, Y., Sun, X., & Wang, Y. Role of C4 carbon fixation in Ulva prolifera, the macroalga responsible for the world's largest green tides. Communications Biology, 3(1), (2020): 494, doi:10.1038/s42003-020-01225-4.
    Description: Most marine algae preferentially assimilate CO2 via the Calvin-Benson Cycle (C3) and catalyze HCO3− dehydration via carbonic anhydrase (CA) as a CO2-compensatory mechanism, but certain species utilize the Hatch-Slack Cycle (C4) to enhance photosynthesis. The occurrence and importance of the C4 pathway remains uncertain, however. Here, we demonstrate that carbon fixation in Ulva prolifera, a species responsible for massive green tides, involves a combination of C3 and C4 pathways, and a CA-supported HCO3− mechanism. Analysis of CA and key C3 and C4 enzymes, and subsequent analysis of δ13C photosynthetic products showed that the species assimilates CO2 predominately via the C3 pathway, uses HCO3− via the CA mechanism at low CO2 levels, and takes advantage of high irradiance using the C4 pathway. This active and multi-faceted carbon acquisition strategy is advantageous for the formation of massive blooms, as thick floating mats are subject to intense surface irradiance and CO2 limitation.
    Description: This work was supported by the State Key Project of Research and Development Plan, Ministry of Science and Technology of the Peopleʼs Republic of China (2016YFC1402106). Support for D.M.A. provided by the Woods Hole Oceanographic Institution—Ocean University of China Cooperative Research Initiative. We thank Dr. Juntian Xu, Jing Ma, Ying Li, and Chenglong Ji for assisting culture experiments and sample analysis.
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  • 43
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Jiao, F., Cannon, K. S., Lin, Y. C., Gladfelter, A. S., & Scheuring, S. The hierarchical assembly of septins revealed by high-speed AFM. Nature Communications, 11(1), (2020): 5062, doi:10.1038/s41467-020-18778-x.
    Description: Septins are GTP-binding proteins involved in diverse cellular processes including division and membrane remodeling. Septins form linear, palindromic heteromeric complexes that can assemble in filaments and higher-order structures. Structural studies revealed various septin architectures, but questions concerning assembly-dynamics and -pathways persist. Here we used high-speed atomic force microscopy (HS-AFM) and kinetic modeling which allowed us to determine that septin filament assembly was a diffusion-driven process, while formation of higher-order structures was complex and involved self-templating. Slightly acidic pH and increased monovalent ion concentrations favor filament-assembly, -alignment and -pairing. Filament-alignment and -pairing further favored diffusion-driven assembly. Pairing is mediated by the septin N-termini face, and may occur symmetrically or staggered, likely important for the formation of higher-order structures of different shapes. Multilayered structures are templated by the morphology of the underlying layers. The septin C-termini face, namely the C-terminal extension of Cdc12, may be involved in membrane binding.
    Description: We thank J. Thorner for the generous gift of the CTE mutant plasmids. K.S.C. was supported in part by a grant from NIGMS under award T32 GM119999 and A.S.G., F.J. and S.S. were supported by NIH RO1 GM130934.
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  • 44
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Winkler, T. S., van Hengstum, P. J., Donnelly, J. P., Wallace, E. J., Sullivan, R. M., MacDonald, D., & Albury, N. A. Revising evidence of hurricane strikes on Abaco Island (the Bahamas) over the last 700 years. Scientific Reports, 10(1), (2020): 16556, doi:10.1038/s41598-020-73132-x.
    Description: The northern Bahamas have experienced more frequent intense-hurricane impacts than almost anywhere else in the Atlantic since 1850 CE. In 2019, category 5 (Saffir-Simpson scale) Hurricane Dorian demonstrated the destructive potential of these natural hazards. Problematically, determining whether high hurricane activity levels remained constant through time is difficult given the short observational record (〈 170 years). We present a 700-year long, near-annually resolved stratigraphic record of hurricane passage near Thatchpoint Blue Hole (TPBH) on Abaco Island, The Bahamas. Using longer sediment cores (888 cm) and more reliable age-control, this study revises and temporally expands a previous study from TPBH that underestimated the sedimentation rate. TPBH records at least 13 ≥ category 2 hurricanes per century between 1500 to 1670 CE, which exceeds the 9 ≥ category 2 hurricanes per century within 50 km of TPBH since 1850 CE. The eastern United States also experienced frequent hurricanes from 1500 to 1670 CE, but frequency was depressed elsewhere in the Atlantic Ocean. This suggests that spatial heterogeneity in Atlantic hurricane activity since 1850 CE could have persisted throughout the last millennium. This heterogeneity is impacted by climatic and stochastic forcing, but additional high-resolution paleo-hurricane reconstructions are required to assess the mechanisms that impact regional variability.
    Description: Field support was provided by Jody Albury and the staff of Friends of the Environment in Marsh Harbour, The Bahamas, and technical support was provided was provided by M. Horgan and S. Molodtsov. Funding for this project was provided by NSF Awards OCE-1356509, OCE-1356708, OCE-1854917, OCE-1903616, and ICER-1854980. The open access publishing fees for this article have been covered by the Texas A&M University Open Access to Knowledge Fund (OAKFund), supported by the University Libraries.
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  • 45
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 50(4), (2020): 887-905, doi:10.1175/JPO-D-19-0110.1.
    Description: The Equatorial Undercurrent (EUC) encounters the Galápagos Archipelago on the equator as it flows eastward across the Pacific. The impact of the Galápagos Archipelago on the EUC in the eastern equatorial Pacific remains largely unknown. In this study, the path of the EUC as it reaches the Galápagos Archipelago is measured directly using high-resolution observations obtained by autonomous underwater gliders. Gliders were deployed along three lines that define a closed region with the Galápagos Archipelago as the eastern boundary and 93°W from 2°S to 2°N as the western boundary. Twelve transects were simultaneously occupied along the three lines during 52 days in April–May 2016. Analysis of individual glider transects and average sections along each line show that the EUC splits around the Galápagos Archipelago. Velocity normal to the transects is used to estimate net horizontal volume transport into the volume. Downward integration of the net horizontal transport profile provides an estimate of the time- and areal-averaged vertical velocity profile over the 52-day time period. Local maxima in vertical velocity occur at depths of 25 and 280 m with magnitudes of (1.7 ± 0.6) × 10−5 m s−1 and (8.0 ± 1.6) × 10−5 m s−1, respectively. Volume transport as a function of salinity indicates that water crossing 93°W south (north) of 0.4°S tends to flow around the south (north) side of the Galápagos Archipelago. Comparisons are made between previous observational and modeling studies with differences attributed to effects of the strong 2015/16 El Niño event, the annual cycle of local winds, and varying longitudes between studies of the equatorial Pacific.
    Description: This work was supported by National Science Foundation (Grants OCE-1232971 and OCE-1233282) and the NASA Earth and Space Science Fellowship Program (Grant 80NSSC17K0443).
    Keywords: Tropics ; Boundary currents ; Topographic effects ; Transport ; Upwelling/downwelling ; In situ oceanic observations
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  • 46
    Publication Date: 2022-05-26
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Mayfield, K. K., Eisenhauer, A., Santiago Ramos, D. P., Higgins, J. A., Horner, T. J., Auro, M., Magna, T., Moosdorf, N., Charette, M. A., Gonneea, M. E., Brady, C. E., Komar, N., Peucker-Ehrenbrink, B., & Paytan, A. Groundwater discharge impacts marine isotope budgets of Li, Mg, Ca, Sr, and Ba. Nature Communications, 12(1), (2021): 148-020-20248-3, doi:10.1038/s41467-020-20248-3.
    Description: Groundwater-derived solute fluxes to the ocean have long been assumed static and subordinate to riverine fluxes, if not neglected entirely, in marine isotope budgets. Here we present concentration and isotope data for Li, Mg, Ca, Sr, and Ba in coastal groundwaters to constrain the importance of groundwater discharge in mediating the magnitude and isotopic composition of terrestrially derived solute fluxes to the ocean. Data were extrapolated globally using three independent volumetric estimates of groundwater discharge to coastal waters, from which we estimate that groundwater-derived solute fluxes represent, at a minimum, 5% of riverine fluxes for Li, Mg, Ca, Sr, and Ba. The isotopic compositions of the groundwater-derived Mg, Ca, and Sr fluxes are distinct from global riverine averages, while Li and Ba fluxes are isotopically indistinguishable from rivers. These differences reflect a strong dependence on coastal lithology that should be considered a priority for parameterization in Earth-system models.
    Description: We thank A. Beck, H. Dulai, I. Santos, C. Benitez-Nelson, W. Moore, A. Martin, and H. Windom for sample access. We also thank A. Kolevica, A. Heuser, H. Pryer, J. Middleton, R. Franks, F. Lon, N. Slater, and O. Šebek for their laboratory and analytical assistance. This material is based upon research supported by the National Science Foundation Graduate Research Fellowship Program and an internship provided through the U.S. Geological Survey Graduate Research Internship Program (GRIP). This research was also supported by grants from: the German Academic Exchange Service (DAAD), Northern California chapter of the Achievement Rewards for College Scientists Foundation, International Association of GeoChemistry, Geological Society of America, Northern California Geological Society, Myers Trust, Friends of Long Marine Lab, and UC MEXUS (to K.K.M.). We acknowledge funding from EU-ITN Horizon project 643084 (to A.E. and T.M.) and NSF grant Award Number 1259440 (to A.P.). We also acknowledge funding from NSF grant award number OCE-1736949 (to T.J.H.). Any use of trade, firm or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.
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  • 47
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 50(10), (2020): 2849-2871, https://doi.org/10.1175/JPO-D-20-0086.1.
    Description: The structure, transport, and seasonal variability of the West Greenland boundary current system near Cape Farewell are investigated using a high-resolution mooring array deployed from 2014 to 2018. The boundary current system is comprised of three components: the West Greenland Coastal Current, which advects cold and fresh Upper Polar Water (UPW); the West Greenland Current, which transports warm and salty Irminger Water (IW) along the upper slope and UPW at the surface; and the Deep Western Boundary Current, which advects dense overflow waters. Labrador Sea Water (LSW) is prevalent at the seaward side of the array within an offshore recirculation gyre and at the base of the West Greenland Current. The 4-yr mean transport of the full boundary current system is 31.1 ± 7.4 Sv (1 Sv ≡ 106 m3 s−1), with no clear seasonal signal. However, the individual water mass components exhibit seasonal cycles in hydrographic properties and transport. LSW penetrates the boundary current locally, through entrainment/mixing from the adjacent recirculation gyre, and also enters the current upstream in the Irminger Sea. IW is modified through air–sea interaction during winter along the length of its trajectory around the Irminger Sea, which converts some of the water to LSW. This, together with the seasonal increase in LSW entering the current, results in an anticorrelation in transport between these two water masses. The seasonality in UPW transport can be explained by remote wind forcing and subsequent adjustment via coastal trapped waves. Our results provide the first quantitatively robust observational description of the boundary current in the eastern Labrador Sea.
    Description: A.P., R.S.P., F.B., D.J.T., and A.L.R. were funded by Grants OCE-1259618 and OCE-1756361 from the National Science Foundation. I.L.B, F.S., and J.H. were supported by U.S. National Science Foundation Grants OCE-1258823 and OCE-1756272. Mooring data from MA2 was funded by the European Union 7th Framework Programme (FP7 2007-2013) under Grant 308299 (NACLIM) and the Horizon 2020 research and innovation program under Grant 727852 (Blue-Action). J.K. and M.O. acknowledge EU Horizon 2020 funding Grants 727852 (Blue-action) and 862626 (EuroSea) and from the German Ministry of Research and Education (RACE Program). G.W.K.M. acknowledges funding from the Natural Sciences and Engineering Research Council.
    Keywords: Boundary currents ; Convection ; Deep convection ; Transport ; In situ oceanic observations ; Seasonal cycle
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  • 48
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Bulletin of the American Meteorological Society 101(11), (2020): E1996-E2004, https://doi.org/10.1175/BAMS-D-19-0305.1.
    Description: A long-standing challenge in oceanography is the observing, modeling, and prediction of vertical transport, which links the sunlit and atmospherically mediated surface boundary layer with the deeper ocean. Vertical motions play a critical role in the exchange of heat, freshwater, and biogeochemical tracers between the surface and the ocean interior. The most intense vertical velocities occur at horizontal scales less than 10 km, making them difficult to observe in the ocean and to resolve in models. Understanding how finescale turbulent motions and 0.1–10 km submesoscale processes contribute to the large-scale budgets of nutrients, oxygen, carbon, and heat and affect sea surface temperature, the air–sea exchange of gases, and the carbon cycle is one of the key challenges in oceanography.
    Description: CALYPSO is a Departmental Research Initiative (DRI) funded by the U.S. Office of Naval Research (ONR). It is a collaborative program involving more than 30 scientists and students and multiple institutions in the United States, Spain, and Italy. Measurements were conducted from the NRV Alliance, Pourquoi Pas?, and SOCIB. We are grateful to the captains and crews of these research vessels and the technical and scientific staff involved in making measurements, running models, analyzing data, and providing support.
    Description: 2021-05-01
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  • 49
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Skottene, E., Tarrant, A. M., Altin, D., Olsen, R. E., Choquet, M., & Kvile, K. O. Lipid metabolism in Calanus finmarchicus is sensitive to variations in predation risk and food availability. Scientific Reports, 10(1),(2020): 22322, https://doi.org/10.1038/s41598-020-79165-6.
    Description: Late developmental stages of the marine copepods in the genus Calanus can spend extended periods in a dormant stage (diapause) that is preceded by the accumulation of large lipid stores. We assessed how lipid metabolism during development from the C4 stage to adult is altered in response to predation risk and varying food availability, to ultimately understand more of the metabolic processes during development in Calanus copepods. We used RNA sequencing to assess if perceived predation risk in combination with varied food availability affects expression of genes associated with lipid metabolism and diapause preparation in C. finmarchicus. The lipid metabolism response to predation risk differed depending on food availability, time and life stage. Predation risk caused upregulation of lipid catabolism with high food, and downregulation with low food. Under low food conditions, predation risk disrupted lipid accumulation. The copepods showed no clear signs of diapause preparation, supporting earlier observations of the importance of multiple environmental cues in inducing diapause in C. finmarchicus. This study demonstrates that lipid metabolism is a sensitive endpoint for the interacting environmental effects of predation pressure and food availability. As diapause may be controlled by lipid accumulation, our findings may contribute towards understanding processes that can ultimately influence diapause timing.
    Description: ES was funded by the Department of Biology, Norwegian University of Science and Technology. Support for AMT was provided by the National Science Foundation (NSF) OPP-1746087. KØK was funded by VISTA—a basic research program in collaboration between The Norwegian Academy of Science and Letters, and Equinor. The RNA seq work was provided by the Genomics Core Facility (GCF). GCF is funded by the Faculty of Medicine and Health Sciences at NTNU and Central Norway Regional Health Authority. The authors thank Lotte Thommesen for help conducting the experiment, Mari-Ann Østensen for performing RNA extraction with ES and preparing cDNA libraries for Illumina RNAseq, and the EMBRC-ERIC Laboratory for Low Level Trophic Interactions at NTNU SeaLab for access to the Calanus finmarchicus culture.
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  • 50
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 50(4), (2020): 1045-1064, doi:10.1175/JPO-D-19-0137.1.
    Description: Three simulations of the circulation in the Gulf of Mexico (the “Gulf”) using different numerical general circulation models are compared with results of recent large-scale observational campaigns conducted throughout the deep (〉1500 m) Gulf. Analyses of these observations have provided new understanding of large-scale mean circulation features and variability throughout the deep Gulf. Important features include cyclonic flow along the continental slope, deep cyclonic circulation in the western Gulf, a counterrotating pair of cells under the Loop Current region, and a cyclonic cell to the south of this pair. These dominant circulation features are represented in each of the ocean model simulations, although with some obvious differences. A striking difference between all the models and the observations is that the simulated deep eddy kinetic energy under the Loop Current region is generally less than one-half of that computed from observations. A multidecadal integration of one of these numerical simulations is used to evaluate the uncertainty of estimates of velocity statistics in the deep Gulf computed from limited-length (4 years) observational or model records. This analysis shows that the main deep circulation features identified from the observational studies appear to be robust and are not substantially impacted by variability on time scales longer than the observational records. Differences in strengths and structures of the circulation features are identified, however, and quantified through standard error analysis of the statistical estimates using the model solutions.
    Description: This work was supported by the Gulf Research Program of the National Academy of Sciences under Awards 2000006422 and 2000009966. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Gulf Research Program or the National Academy of Sciences. The authors acknowledge the GLORYS project for providing the ocean reanalysis data used in the ROMS simulation. GLORYS is jointly conducted by MERCATOR OCEAN, CORIOLIS, and CNRS/INSU. Installation, recovery, data acquisition, and processing of the CANEK group current-meter moorings were possible because of CICESE-PetróleosMexicanos Grant PEP-CICESE 428229851 and the dedicated work of the crew of the B/O Justo Sierra and scientists of the CANEK group. The authors thank Dr. Aljaz Maslo, CICESE, for assistance with analysis of model data. The Bureau of Ocean Energy Management (BOEM), U.S. Dept. of the Interior, provided funding for the Lagrangian Study of the Deep Circulation in the Gulf of Mexico and the Observations and Dynamics of the Loop Current study. HYCOM simulation data are available from the HYCOM data server (https://www.hycom.org/data/goml0pt04/expt-02pt2), MITgcm data are available from the ECCO data server (http://ecco.ucsd.edu/gom_results2.html), and the ROMS simulation data are available from GRIIDC (NA.x837.000:0001).
    Keywords: Ocean circulation ; Abyssal circulation ; Bottom currents/bottom water ; Eddies ; Ocean models
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  • 51
    Publication Date: 2022-10-14
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Marshall, T., Granger, J., Casciotti, K. L., Dahnke, K., Emeis, K.-C., Marconi, D., McIlvin, M. R., Noble, A. E., Saito, M. A., Sigman, D. M., & Fawcett, S. E. The Angola Gyre is a hotspot of dinitrogen fixation in the South Atlantic Ocean. Communications Earth & Environment, 3(1), (2022): 151, https://doi.org/10.1038/s43247-022-00474-x.
    Description: Biological dinitrogen fixation is the major source of new nitrogen to marine systems and thus essential to the ocean’s biological pump. Constraining the distribution and global rate of dinitrogen fixation has proven challenging owing largely to uncertainty surrounding the controls thereon. Existing South Atlantic dinitrogen fixation rate estimates vary five-fold, with models attributing most dinitrogen fixation to the western basin. From hydrographic properties and nitrate isotope ratios, we show that the Angola Gyre in the eastern tropical South Atlantic supports the fixation of 1.4–5.4 Tg N.a−1, 28-108% of the existing (highly uncertain) estimates for the basin. Our observations contradict model diagnoses, revealing a substantial input of newly-fixed nitrogen to the tropical eastern basin and no dinitrogen fixation west of 7.5˚W. We propose that dinitrogen fixation in the South Atlantic occurs in hotspots controlled by the overlapping biogeography of excess phosphorus relative to nitrogen and bioavailable iron from margin sediments. Similar conditions may promote dinitrogen fixation in analogous ocean regions. Our analysis suggests that local iron availability causes the phosphorus-driven coupling of oceanic dinitrogen fixation to nitrogen loss to vary on a regional basis.
    Description: This work was supported by the South African National Research Foundation (114673 and 130826 to T.M., 115335, 116142 and 129320 to S.E.F.); the US National Science Foundation (CAREER award, OCE-1554474 to J.G., OCE-1736652 to D.M.S. and K.L.C., OCE-05-26277 to K.L.C.); the German Federal Agency for Education and Research (DAAD-SPACES 57371082 to T.M.); the Royal Society (FLAIR fellowship to S.E.F.); and the University of Cape Town (T.M., J.G., S.E.F.). The authors also recognize the support of the South African Department of Science and Innovation’s Biogeochemistry Research Infrastructure Platform (BIOGRIP).
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  • 52
    Publication Date: 2022-10-14
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Houskeeper, H. F., Rosenthal, I. S., Cavanaugh, K. C., Pawlak, C., Trouille, L., Byrnes, J. E. K., Bell, T. W., & Cavanaugh, K. C. Automated satellite remote sensing of giant kelp at the Falkland Islands (Islas Malvinas). Plos One, 17(1), (2022): e0257933, https://doi.org/10.1371/journal.pone.0257933.
    Description: Giant kelp populations that support productive and diverse coastal ecosystems at temperate and subpolar latitudes of both hemispheres are vulnerable to changing climate conditions as well as direct human impacts. Observations of giant kelp forests are spatially and temporally uneven, with disproportionate coverage in the northern hemisphere, despite the size and comparable density of southern hemisphere kelp forests. Satellite imagery enables the mapping of existing and historical giant kelp populations in understudied regions, but automating the detection of giant kelp using satellite imagery requires approaches that are robust to the optical complexity of the shallow, nearshore environment. We present and compare two approaches for automating the detection of giant kelp in satellite datasets: one based on crowd sourcing of satellite imagery classifications and another based on a decision tree paired with a spectral unmixing algorithm (automated using Google Earth Engine). Both approaches are applied to satellite imagery (Landsat) of the Falkland Islands or Islas Malvinas (FLK), an archipelago in the southern Atlantic Ocean that supports expansive giant kelp ecosystems. The performance of each method is evaluated by comparing the automated classifications with a subset of expert-annotated imagery (8 images spanning the majority of our continuous timeseries, cumulatively covering over 2,700 km of coastline, and including all relevant sensors). Using the remote sensing approaches evaluated herein, we present the first continuous timeseries of giant kelp observations in the FLK region using Landsat imagery spanning over three decades. We do not detect evidence of long-term change in the FLK region, although we observe a recent decline in total canopy area from 2017–2021. Using a nitrate model based on nearby ocean state measurements obtained from ships and incorporating satellite sea surface temperature products, we find that the area of giant kelp forests in the FLK region is positively correlated with the nitrate content observed during the prior year. Our results indicate that giant kelp classifications using citizen science are approximately consistent with classifications based on a state-of-the-art automated spectral approach. Despite differences in accuracy and sensitivity, both approaches find high interannual variability that impedes the detection of potential long-term changes in giant kelp canopy area, although recent canopy area declines are notable and should continue to be monitored carefully.
    Description: This work was funded by the National Aeronautics and Space Administration as part of the Citizen Science for Earth Systems Program (https://earthdata.nasa.gov/esds/competitive-programs/csesp) with grant #80NSSC18M0103 (awarded to JEKB), which also provided salary to HFH, and by the National Science Foundation through the Santa Barbara Coastal Long-Term Environmental Research (https://sbclter.msi.ucsb.edu) program with grants #OCE 0620276 and 1232779. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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  • 53
    Publication Date: 2022-10-12
    Description: Author Posting. © American Meteorological Society, 2022. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 52(10), (2022): 2431-2444, https://doi.org/10.1175/jpo-d-22-0024.1.
    Description: A three-dimensional inertial model that conserves quasigeostrophic potential vorticity is proposed for wind-driven coastal upwelling along western boundaries. The dominant response to upwelling favorable winds is a surface-intensified baroclinic meridional boundary current with a subsurface countercurrent. The width of the current is not the baroclinic deformation radius but instead scales with the inertial boundary layer thickness while the depth scales as the ratio of the inertial boundary layer thickness to the baroclinic deformation radius. Thus, the boundary current scales depend on the stratification, wind stress, Coriolis parameter, and its meridional variation. In contrast to two-dimensional wind-driven coastal upwelling, the source waters that feed the Ekman upwelling are provided over the depth scale of this baroclinic current through a combination of onshore barotropic flow and from alongshore in the narrow boundary current. Topography forces an additional current whose characteristics depend on the topographic slope and width. For topography wider than the inertial boundary layer thickness the current is bottom intensified, while for narrow topography the current is wave-like in the vertical and trapped over the topography within the inertial boundary layer. An idealized primitive equation numerical model produces a similar baroclinic boundary current whose vertical length scale agrees with the theoretical scaling for both upwelling and downwelling favorable winds.
    Description: This research is supported in part by the China Scholarship Council (201906330102). H. G. is financially supported by the China Scholarship Council to study at WHOI for 2 years as a guest student. M.S. is supported by the National Science Foundation Grant OCE-1922538. Z. C. is supported by the ‘Taishan/Aoshan’ Talents program (2017ASTCPES05) the Fundamental Research Funds for the Central Universities (202072001).
    Description: 2023-03-30
    Keywords: Ekman pumping/transport ; Upwelling/downwelling ; Coastal flows
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  • 54
    Publication Date: 2022-10-12
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Struve, T., Wilson, D., Hines, S., Adkins, J., & van de Flierdt, T. A deep Tasman outflow of Pacific waters during the last glacial period. Nature Communications, 13(1), (2022): 3763, https://doi.org/10.1038/s41467-022-31116-7.
    Description: The interoceanic exchange of water masses is modulated by flow through key oceanic choke points in the Drake Passage, the Indonesian Seas, south of Africa, and south of Tasmania. Here, we use the neodymium isotope signature (εNd) of cold-water coral skeletons from intermediate depths (1460‒1689 m) to trace circulation changes south of Tasmania during the last glacial period. The key feature of our dataset is a long-term trend towards radiogenic εNd values of ~−4.6 during the Last Glacial Maximum and Heinrich Stadial 1, which are clearly distinct from contemporaneous Southern Ocean εNd of ~−7. When combined with previously published radiocarbon data from the same corals, our results indicate that a unique radiogenic and young water mass was present during this time. This scenario can be explained by a more vigorous Pacific overturning circulation that supported a deeper outflow of Pacific waters, including North Pacific Intermediate Water, through the Tasman Sea.
    Description: The authors acknowledge financial support from the Grantham Institute of Climate Change and the Environment (T.v.d.F. and T.S.), the Ministry for Science and Culture of the State of Lower Saxony (T.S.), Marie Curie Reintegration grant IRG 230828 (T.v.d.F.), Leverhulme Trust grant RPG-398 (T.v.d.F.), Natural Environment Research Council grants NE/F016751/1 (T.v.d.F.), NE/N001141/1 (T.v.d.F. and D.J.W.), and NE/T011440/1 (D.J.W.), and National Science Foundation grant OCE-1503129 (J.F.A. and S.K.V.H.). Open Access funding is enabled by the DFG open access publication fund and the Carl von Ossietzky University Oldenburg.
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  • 55
    Publication Date: 2022-10-12
    Description: Author Posting. © American Meteorological Society, 2022. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 52(10), (2022): 2325–2341, https://doi.org/10.1175/jpo-d-21-0015.1.
    Description: The ocean surface boundary layer is a gateway of energy transfer into the ocean. Wind-driven shear and meteorologically forced convection inject turbulent kinetic energy into the surface boundary layer, mixing the upper ocean and transforming its density structure. In the absence of direct observations or the capability to resolve subgrid-scale 3D turbulence in operational ocean models, the oceanography community relies on surface boundary layer similarity scalings (BLS) of shear and convective turbulence to represent this mixing. Despite their importance, near-surface mixing processes (and ubiquitous BLS representations of these processes) have been undersampled in high-energy forcing regimes such as the Southern Ocean. With the maturing of autonomous sampling platforms, there is now an opportunity to collect high-resolution spatial and temporal measurements in the full range of forcing conditions. Here, we characterize near-surface turbulence under strong wind forcing using the first long-duration glider microstructure survey of the Southern Ocean. We leverage these data to show that the measured turbulence is significantly higher than standard shear-convective BLS in the shallower parts of the surface boundary layer and lower than standard shear-convective BLS in the deeper parts of the surface boundary layer; the latter of which is not easily explained by present wave-effect literature. Consistent with the CBLAST (Coupled Boundary Layers and Air Sea Transfer) low winds experiment, this bias has the largest magnitude and spread in the shallowest 10% of the actively mixing layer under low-wind and breaking wave conditions, when relatively low levels of turbulent kinetic energy (TKE) in surface regime are easily biased by wave events.
    Description: This paper is VIMS Contribution 4103. Computational resources were provided by the VIMS Ocean-Atmosphere and Climate Change Research Fund. AUSSOM was supported by the OCE Division of the National Science Foundation (1558639).
    Keywords: Turbulence ; Wind shear ; Boundary layer ; Parameterization
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  • 56
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Nielsen, S. G., Bekaert, D. V., & Auro, M. Isotopic evidence for the formation of the moon in a canonical giant impact. Nature Communications, 12(1), (2021): 1817, https://doi.org/10.1038/s41467-021-22155-7.
    Description: Isotopic measurements of lunar and terrestrial rocks have revealed that, unlike any other body in the solar system, the Moon is indistinguishable from the Earth for nearly every isotopic system. This observation, however, contradicts predictions by the standard model for the origin of the Moon, the canonical giant impact. Here we show that the vanadium isotopic composition of the Moon is offset from that of the bulk silicate Earth by 0.18 ± 0.04 parts per thousand towards the chondritic value. This offset most likely results from isotope fractionation on proto-Earth during the main stage of terrestrial core formation (pre-giant impact), followed by a canonical giant impact where ~80% of the Moon originates from the impactor of chondritic composition. Our data refute the possibility of post-giant impact equilibration between the Earth and Moon, and implies that the impactor and proto-Earth mainly accreted from a common isotopic reservoir in the inner solar system.
    Description: This study was funded by NASA Emerging Worlds grant NNX16AD36G to S.G.N. We thank NASA-JSC, Tony Irving, and Thorsten Kleine for access to meteorite and Apollo mission samples. US Antarctic meteorite samples are recovered by the Antarctic Search for Meteorites (ANSMET) program, which has been funded by NSF and NASA, and characterized and curated by the Astromaterials Curation Office at NASA Johnson Space Center and the Department of Mineral Sciences of the Smithsonian Institution. J. Blusztajn is thanked for help with mass spectrometry support at WHOI.
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  • 57
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Wei, C., Hoffmann-Kuhnt, M., Au, W. W. L., Ho, A. Z. H., Matrai, E., Feng, W., Ketten, D. R., & Zhang, Y. Possible limitations of dolphin echolocation: a simulation study based on a cross-modal matching experiment. Scientific Reports, 11(1), (2021): 6689, https://doi.org/10.1038/s41598-021-85063-2.
    Description: Dolphins use their biosonar to discriminate objects with different features through the returning echoes. Cross-modal matching experiments were conducted with a resident bottlenose dolphin (Tursiops aduncus). Four types of objects composed of different materials (water-filled PVC pipes, air-filled PVC pipes, foam ball arrays, and PVC pipes wrapped in closed-cell foam) were used in the experiments, respectively. The size and position of the objects remained the same in each case. The data collected in the experiment showed that the dolphin’s matching accuracy was significantly different across the cases. To gain insight into the underlying mechanism in the experiments, we used finite element methods to construct two-dimensional target detection models of an echolocating dolphin in the vertical plane, based on computed tomography scan data. The acoustic processes of the click’s interaction with the objects and the surrounding media in the four cases were simulated and compared. The simulation results provide some possible explanations for why the dolphin performed differently when discriminating the objects that only differed in material composition in the previous matching experiments.
    Description: One of the authors, Wei. C is supported by a Forrest Research Foundation Fellowship. Support for D. Ketten for this effort was provided by the Joint Industry Programme and by the Helmholtz Foundation. This work was also supported by the Hawaii Institute of Marine Biology (HIMB) contribution No. 1630 and School of Ocean and Earth Science and Technology (SOEST) contribution No. 9452.
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    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Tani, T., Koike-Tani, M., Tran, M. T., Shribak, M., & Levic, S. Postnatal structural development of mammalian basilar membrane provides anatomical basis for the maturation of tonotopic maps and frequency tuning. Scientific Reports, 11(1), (2021): 7581, https://doi.org/10.1038/s41598-021-87150-w.
    Description: The basilar membrane (BM) of the mammalian cochlea constitutes a spiraling acellular ribbon that is intimately attached to the organ of Corti. Its graded stiffness, increasing from apex to the base of the cochlea provides the mechanical basis for sound frequency analysis. Despite its central role in auditory signal transduction, virtually nothing is known about the BM’s structural development. Using polarized light microscopy, the present study characterized the architectural transformations of freshly dissected BM at time points during postnatal development and maturation. The results indicate that the BM structural elements increase progressively in size, becoming radially aligned and more tightly packed with maturation and reach the adult structural signature by postnatal day 20 (P20). The findings provide insight into structural details and developmental changes of the mammalian BM, suggesting that BM is a dynamic structure that changes throughout the life of an animal.
    Description: This work was supported by Medical Research Council grant MR/ N004299/1 (to SL), University of Sussex Research Innovation Funds (to SL), Brighton and Sussex Medical School research funds (to SL), National Institute of General Medical Sciences, NIH grant R01 GM101701 (to MS), NIH R01 GM100160 (to TT) and KAKENHI JP18K19962 (to TT).
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  • 59
    Publication Date: 2022-05-27
    Description: Author Posting. © American Meteorological Society, 2021. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 51(1), (2021): 19-35, https://doi.org/10.1175/JPO-D-19-0233.1.
    Description: In the Beaufort Sea in September of 2015, concurrent mooring and microstructure observations were used to assess dissipation rates in the vicinity of 72°35′N, 145°1′W. Microstructure measurements from a free-falling profiler survey showed very low [O(10−10) W kg−1] turbulent kinetic energy dissipation rates ε. A finescale parameterization based on both shear and strain measurements was applied to estimate the ratio of shear to strain Rω and ε at the mooring location, and a strain-based parameterization was applied to the microstructure survey (which occurred approximately 100 km away from the mooring site) for direct comparison with microstructure results. The finescale parameterization worked well, with discrepancies ranging from a factor of 1–2.5 depending on depth. The largest discrepancies occurred at depths with high shear. Mean Rω was 17, and Rω showed high variability with values ranging from 3 to 50 over 8 days. Observed ε was slightly elevated (factor of 2–3 compared with a later survey of 11 profiles taken over 3 h) from 25 to 125 m following a wind event which occurred at the beginning of the mooring deployment, reaching a maximum of ε= 6 × 10−10 W kg−1 at 30-m depth. Velocity signals associated with near-inertial waves (NIWs) were observed at depths greater than 200 m, where the Atlantic Water mass represents a reservoir of oceanic heat. However, no evidence of elevated ε or heat fluxes was observed in association with NIWs at these depths in either the microstructure survey or the finescale parameterization estimates.
    Description: This work was supported by NSF Grants PLR 14-56705 and PLR-1303791 and by NSF Graduate Research Fellowship Grant DGE-1650112.
    Keywords: Ocean ; Arctic ; Internal waves ; Turbulence ; Diapycnal mixing
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  • 60
    Publication Date: 2022-05-27
    Description: Author Posting. © American Meteorological Society, 2021. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 51(1),(2021): 3-17, https://doi.org/10.1175/JPO-D-20-0064.1.
    Description: The strong El Niño of 2014–16 was observed west of the Galápagos Islands through sustained deployment of underwater gliders. Three years of observations began in October 2013 and ended in October 2016, with observations at longitudes 93° and 95°W between latitudes 2°N and 2°S. In total, there were over 3000 glider-days of data, covering over 50 000 km with over 12 000 profiles. Coverage was superior closer to the Galápagos on 93°W, where gliders were equipped with sensors to measure velocity as well as temperature, salinity, and pressure. The repeated glider transects are analyzed to produce highly resolved mean sections and maps of observed variables as functions of time, latitude, and depth. The mean sections reveal the structure of the Equatorial Undercurrent (EUC), the South Equatorial Current, and the equatorial front. The mean fields are used to calculate potential vorticity Q and Richardson number Ri. Gradients in the mean are strong enough to make the sign of Q opposite to that of planetary vorticity and to have Ri near unity, suggestive of mixing. Temporal variability is dominated by the 2014–16 El Niño, with the arrival of depressed isopycnals documented in 2014 and 2015. Increases in eastward velocity advect anomalously salty water and are uncorrelated with warm temperatures and deep isopycnals. Thus, vertical advection is important to changes in heat, and horizontal advection is relevant to changes in salt. Implications of this work include possibilities for future research, model assessment and improvement, and sustained observations across the equatorial Pacific.
    Description: We gratefully acknowledge the support of the National Science Foundation (OCE-1232971, OCE-1233282) and the Ocean Observing and Monitoring Division of the National Oceanographic and Atmospheric Administration (NA13OAR4830216).
    Keywords: Ocean ; Tropics ; Currents ; El Nino ; In situ oceanic observations
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  • 61
    Publication Date: 2022-05-27
    Description: Author Posting. © American Meteorological Society, 2021. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of the Atmospheric and Oceanic Technology 38(1), (2021): 3-16, https://doi.org/10.1175/JTECH-D-20-0110.1.
    Description: Airborne expendable bathythermographs (AXBTs) are air-launched, single-use temperature–depth probes that telemeter temperature observations as VHF-modulated frequencies. This study describes the AXBT Real-Time Editing System (ARES), which is composed of two components: the ARES Data Acquisition System, which receives telemetered temperature–depth profiles with no external hardware other than a VHF radio receiver, and the ARES Profile Editing System, which quality controls AXBT temperature–depth profiles. The ARES Data Acquisition System performs fast Fourier transforms on windowed segments of the demodulated signal transmitted from the AXBT. For each segment, temperature is determined from peak frequency and depth from elapsed time since profile start. Valid signals are distinguished from noise by comparing peak signal levels and signal-to-noise ratios to predetermined thresholds. When evaluated using 387 profiles, the ARES Data Acquisition System produced temperature–depth profiles nearly identical to those generated using a Sippican MK-21 processor, while reducing the amount of noise from VHF interference included in those profiles. The ARES Profile Editor applies a series of automated checks to identify and correct common profile discrepancies before displaying the profile on an editing interface that provides simple user controls to make additional corrections. When evaluated against 1177 tropical Atlantic and Pacific AXBT profiles, the ARES automated quality control system successfully corrected 87% of the profiles without any required manual intervention. Necessary future work includes improvements to the automated quality control algorithm and algorithm evaluation against a broader dataset of temperature–depth profiles from around the world across all seasons.
    Description: This work was sponsored by the Office of Naval Research (Grants N000141812819 and N0001420WX00345) and the U.S. Navy’s Civilian Institution Office with the MIT–WHOI Joint Program.
    Keywords: Ocean ; In situ oceanic observations ; Profilers, oceanic
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  • 62
    Publication Date: 2022-05-27
    Description: Author Posting. © American Meteorological Society, 2021. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Bulletin of the American Meteorological Society 102(1), (2021): E99-E122, https://doi.org/10.1175/BAMS-D-19-0005.1.
    Description: The Red Sea, home to the second-longest coral reef system in the world, is a vital resource for the Kingdom of Saudi Arabia. The Red Sea provides 90% of the Kingdom’s potable water by desalinization, supporting tourism, shipping, aquaculture, and fishing industries, which together contribute about 10%–20% of the country’s GDP. All these activities, and those elsewhere in the Red Sea region, critically depend on oceanic and atmospheric conditions. At a time of mega-development projects along the Red Sea coast, and global warming, authorities are working on optimizing the harnessing of environmental resources, including renewable energy and rainwater harvesting. All these require high-resolution weather and climate information. Toward this end, we have undertaken a multipronged research and development activity in which we are developing an integrated data-driven regional coupled modeling system. The telescopically nested components include 5-km- to 600-m-resolution atmospheric models to address weather and climate challenges, 4-km- to 50-m-resolution ocean models with regional and coastal configurations to simulate and predict the general and mesoscale circulation, 4-km- to 100-m-resolution ecosystem models to simulate the biogeochemistry, and 1-km- to 50-m-resolution wave models. In addition, a complementary probabilistic transport modeling system predicts dispersion of contaminant plumes, oil spill, and marine ecosystem connectivity. Advanced ensemble data assimilation capabilities have also been implemented for accurate forecasting. Resulting achievements include significant advancement in our understanding of the regional circulation and its connection to the global climate, development, and validation of long-term Red Sea regional atmospheric–oceanic–wave reanalyses and forecasting capacities. These products are being extensively used by academia, government, and industry in various weather and marine studies and operations, environmental policies, renewable energy applications, impact assessment, flood forecasting, and more.
    Description: The development of the Red Sea modeling system is being supported by the Virtual Red Sea Initiative and the Competitive Research Grants (CRG) program from the Office of Sponsored Research at KAUST, Saudi Aramco Company through the Saudi ARAMCO Marine Environmental Center at KAUST, and by funds from KAEC, NEOM, and RSP through Beacon Development Company at KAUST.
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  • 63
    Publication Date: 2022-05-27
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 50(11), (2020): 3267–3294, https://doi.org/10.1175/JPO-D-19-0310.1.
    Description: As part of the Flow Encountering Abrupt Topography (FLEAT) program, an array of pressure-sensor equipped inverted echo sounders (PIESs) was deployed north of Palau where the westward-flowing North Equatorial Current encounters the southern end of the Kyushu–Palau Ridge in the tropical North Pacific. Capitalizing on concurrent observations from satellite altimetry, FLEAT Spray gliders, and shipboard hydrography, the PIESs’ 10-month duration hourly bottom pressure p and round-trip acoustic travel time τ records are used to examine the magnitude and predictability of sea level and pycnocline depth changes and to track signal propagations through the array. Sea level and pycnocline depth are found to vary in response to a range of ocean processes, with their magnitude and predictability strongly process dependent. Signals characterized here comprise the barotropic tides, semidiurnal and diurnal internal tides, southeastward-propagating superinertial waves, westward-propagating mesoscale eddies, and a strong signature of sea level increase and pycnocline deepening associated with the region’s relaxation from El Niño to La Niña conditions. The presence of a broad band of superinertial waves just above the inertial frequency was unexpected and the FLEAT observations and output from a numerical model suggest that these waves detected near Palau are forced by remote winds east of the Philippines. The PIES-based estimates of pycnocline displacement are found to have large uncertainties relative to overall variability in pycnocline depth, as localized deep current variations arising from interactions of the large-scale currents with the abrupt topography around Palau have significant travel time variability.
    Description: Support for this research was provided by Office of Naval Research Grants N00014-16-1-2668, N00014-18-1-2406, N00014-15-1-2488, and N00014-15-1-2622. R.C.M. was additionally supported by the Postdoctoral Scholar Program at the Woods Hole Oceanographic Institution, with funding provided by the Weston Howland Jr. Postdoctoral Scholarship.
    Keywords: Tropics ; Currents ; Eddies ; ENSO ; Internal waves ; Mesoscale processes
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  • 64
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    American Meteorological Society
    Publication Date: 2022-05-27
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 50(11),(2020): 3331–3351, https://doi.org/10.1175/JPO-D-20-0035.1.
    Description: This study examines the generation of warm spiral structures (referred to as spiral streamers here) over Gulf Stream warm-core rings. Satellite sea surface temperature imagery shows spiral streamers forming after warmer water from the Gulf Stream or newly formed warm-core rings impinges onto old warm-core rings and then intrudes into the old rings. Field measurements in April 2018 capture the vertical structure of a warm spiral streamer as a shallow lens of low-density water winding over an old ring. Observations also show subduction on both sides of the spiral streamer, which carries surface waters downward. Idealized numerical model simulations initialized with observed water-mass densities reproduce spiral streamers over warm-core rings and reveal that their formation is a nonlinear submesoscale process forced by mesoscale dynamics. The negative density anomaly of the intruding water causes a density front at the interface between the intruding water and surface ring water, which, through thermal wind balance, drives a local anticyclonic flow. The pressure gradient and momentum advection of the local interfacial flow push the intruding water toward the ring center. The large-scale anticyclonic flow of the ring and the radial motion of the intruding water together form the spiral streamer. The observed subduction on both sides of the spiral streamer is part of the secondary cross-streamer circulation resulting from frontogenesis on the stretching streamer edges. The surface divergence of the secondary circulation pushes the side edges of the streamer away from each other, widens the warm spiral on the surface, and thus enhances its surface signal.
    Description: Authors W. G. Zhang and D. J. McGillicuddy are both supported by the National Science Foundation through Grant OCE 1657803.
    Keywords: Buoyancy ; Eddies ; Frontogenesis/frontolysis ; Mesoscale processes ; Transport ; Vertical motion
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  • 65
    Publication Date: 2022-05-27
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 50(11), (2020): 3235–3251, https://doi.org/10.1175/JPO-D-20-0095.1.
    Description: The dense outflow through Denmark Strait is the largest contributor to the lower limb of the Atlantic meridional overturning circulation, yet a description of the full velocity field across the strait remains incomplete. Here we analyze a set of 22 shipboard hydrographic–velocity sections occupied along the Látrabjarg transect at the Denmark Strait sill, obtained over the time period 1993–2018. The sections provide the first complete view of the kinematic components at the sill: the shelfbreak East Greenland Current (EGC), the combined flow of the separated EGC, and the North Icelandic Jet (NIJ), and the northward-flowing North Icelandic Irminger Current (NIIC). The total mean transport of overflow water is 3.54 ± 0.29 Sv (1 Sv ≡ 106 m3 s−1), comparable to previous estimates. The dense overflow is partitioned in terms of water mass constituents and flow components. The mean transports of the two types of overflow water—Atlantic-origin Overflow Water and Arctic-origin Overflow Water—are comparable in Denmark Strait, while the merged NIJ–separated EGC transports 55% more water than the shelfbreak EGC. A significant degree of water mass exchange takes place between the branches as they converge in Denmark Strait. There are two dominant time-varying configurations of the flow that are characterized as a cyclonic state and a noncyclonic state. These appear to be wind-driven. A potential vorticity analysis indicates that the flow through Denmark Strait is subject to symmetric instability. This occurs at the top of the overflow layer, implying that the mixing/entrainment process that modifies the overflow water begins at the sill.
    Description: Funding for the study was provided by National Science Foundation (NSF) Grants OCE-1259618, OCE-1756361, and OCE-1558742. The German research cruises were financially supported through various EU Projects (e.g. THOR, NACLIM) and national projects (most recently TRR 181 “Energy Transfer in Atmosphere and Ocean” funded by the German Research Foundation and RACE II “Regional Atlantic Circulation and Global Change” funded by the German Federal Ministry for Education and Research). GWKM acknowledges the support of the Natural Sciences and Engineering Research Council of Canada. LP was supported by NSF Grant OCE-1657870.
    Keywords: Currents ; Instability ; Ocean circulation ; Ocean dynamics ; Potential vorticity
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  • 66
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in MacKinnon, J. A., Simmons, H. L., Hargrove, J., Thomson, J., Peacock, T., Alford, M. H., Barton, B., Boury, S., Brenner, S. D., Couto, N., Danielson, S. L., Fine, E. C., Graber, H. C., Guthrie, J., Hopkins, J. E., Jayne, S. R., Jeon, C., Klenz, T., Lee, C. M., Lenn, Y-D., Lucas, A. J., Lund, B., Mahaffey, C., Norman, L., Rainville, L., Smith, M. M., Thomas, L. N., Torres-Valdés, S., & Wood, K. R. A warm jet in a cold ocean. Nature Communications, 12(1), (2021): 2418, https://doi.org/10.1038/s41467-021-22505-5.
    Description: Unprecedented quantities of heat are entering the Pacific sector of the Arctic Ocean through Bering Strait, particularly during summer months. Though some heat is lost to the atmosphere during autumn cooling, a significant fraction of the incoming warm, salty water subducts (dives beneath) below a cooler fresher layer of near-surface water, subsequently extending hundreds of kilometers into the Beaufort Gyre. Upward turbulent mixing of these sub-surface pockets of heat is likely accelerating sea ice melt in the region. This Pacific-origin water brings both heat and unique biogeochemical properties, contributing to a changing Arctic ecosystem. However, our ability to understand or forecast the role of this incoming water mass has been hampered by lack of understanding of the physical processes controlling subduction and evolution of this this warm water. Crucially, the processes seen here occur at small horizontal scales not resolved by regional forecast models or climate simulations; new parameterizations must be developed that accurately represent the physics. Here we present novel high resolution observations showing the detailed process of subduction and initial evolution of warm Pacific-origin water in the southern Beaufort Gyre.
    Description: Support for this work was provided by the US Office of Naval Research Stratified Ocean Dynamics of the Arctic program (grant numbers N000141512903, N000141612378, N000141612377, N000141612379, N0001416123450, N000141612360, N000141612349, N000141812007, N000141812475, and N000141912514). Additional support for biogeochemistry sampling was provided by UK (NERC) and Germany (BMBF) through the Changing Arctic Ocean Program’s ARISE (NE/P006035/1, NE/P006000/2), PEANUTS (NE/R01275X/1, NE/R012547/2, and BMBF 03F0804) projects and the UK-France PhD program DGA/Dstl. Float deployments and hydrographic data compilations were supported in part by North Pacific Research Board grants A91-99a and A91-00a. The deployment of autonomous ocean profilers was supported by ONR, NOAA Research, and the Joint Institute for the Study of the Atmosphere and Ocean (JISAO) under NOAA Cooperative Agreement NA15OAR4320063.
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  • 67
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Sivaguru, M., Todorov, L. G., Fouke, C. E., Munro, C. M. O., Fouke, K. W., Fouke, K. E., Baughman, M. E., & Fouke, B. W. Corals regulate the distribution and abundance of Symbiodiniaceae and biomolecules in response to changing water depth and sea surface temperature. Scientific Reports, 11(1), (2021): 2230, https://doi.org/10.1038/s41598-021-81520-0.
    Description: The Scleractinian corals Orbicella annularis and O. faveolata have survived by acclimatizing to environmental changes in water depth and sea surface temperature (SST). However, the complex physiological mechanisms by which this is achieved remain only partially understood, limiting the accurate prediction of coral response to future climate change. This study quantitatively tracks spatial and temporal changes in Symbiodiniaceae and biomolecule (chromatophores, calmodulin, carbonic anhydrase and mucus) abundance that are essential to the processes of acclimatization and biomineralization. Decalcified tissues from intact healthy Orbicella biopsies, collected across water depths and seasonal SST changes on Curaçao, were analyzed with novel autofluorescence and immunofluorescence histology techniques that included the use of custom antibodies. O. annularis at 5 m water depth exhibited decreased Symbiodiniaceae and increased chromatophore abundances, while O. faveolata at 12 m water depth exhibited inverse relationships. Analysis of seasonal acclimatization of the O. faveolata holobiont in this study, combined with previous reports, suggests that biomolecules are differentially modulated during transition from cooler to warmer SST. Warmer SST was also accompanied by decreased mucus production and decreased Symbiodiniaceae abundance, which is compensated by increased photosynthetic activity enhanced calcification. These interacting processes have facilitated the remarkable resiliency of the corals through geological time.
    Description: Financial support for this work was provided by the Office of Naval Research (N00014-00-1-0609), the Illinois Carl R. Woese Institute for Genomic Biology Undergraduate Summer Research Fellowship, the Illinois Carl R. Woese Institute for Genomic Biology Mark Tracy Fellowship for Translational Research, and the Illinois Department of Molecular and Cellular Biology Jenner Family Summer Research Fellowship and the Edward and Barbara Weil Research Fund provided to the University of Illinois Urbana-Champaign.
    Keywords: Autofluorescence ; Two-photon microscopy ; X-ray Micro CT ; Water Depth ; Bathymetry ; Sea surface temperature ; Symbiodiniaceae symbiont ; chromatophores ; calmodulin ; carbonic anhydrase ; mucus ; Scleractinian corals ; Orbicella annularis ; Orbicella faveolata
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  • 68
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Johnson, M. D., Scott, J. J., Leray, M., Lucey, N., Bravo, L. M. R., Wied, W. L., & Altieri, A. H. Rapid ecosystem-scale consequences of acute deoxygenation on a Caribbean coral reef. Nature Communications, 12(1), (2021): 4522, https://doi.org/10.1038/s41467-021-24777-3.
    Description: Loss of oxygen in the global ocean is accelerating due to climate change and eutrophication, but how acute deoxygenation events affect tropical marine ecosystems remains poorly understood. Here we integrate analyses of coral reef benthic communities with microbial community sequencing to show how a deoxygenation event rapidly altered benthic community composition and microbial assemblages in a shallow tropical reef ecosystem. Conditions associated with the event precipitated coral bleaching and mass mortality, causing a 50% loss of live coral and a shift in the benthic community that persisted a year later. Conversely, the unique taxonomic and functional profile of hypoxia-associated microbes rapidly reverted to a normoxic assemblage one month after the event. The decoupling of ecological trajectories among these major functional groups following an acute event emphasizes the need to incorporate deoxygenation as an emerging stressor into coral reef research and management plans to combat escalating threats to reef persistence.
    Description: M.D.J. was funded by postdoctoral fellow awards from the Smithsonian Institution’s Marine Global Earth Observatory (MarineGEO) and the Smithsonian Tropical Research Institute (STRI); M.L. and N.L. were funded by postdoctoral support from the STRI Office of Fellowships. J.J.S. was funded by a grant from the Gordon and Betty Moore Foundation awarded to STRI and UC Davis (doi:10.37807/GBMF5603). L.M.R.B., W.L.W., and A.H.A. were supported by MarineGEO, a private funder, and STRI funds to A.H.A. Many of the computations were conducted on the Smithsonian High-Performance Cluster (SI/HPC), Smithsonian Institution (doi:10.25572/SIHPC). We thank Rachel Collin for facilities support at the Bocas del Toro Research Station, Plinio Gondola and the research station staff for logistical support, Roman Barco for insight into the functional analyses, Sherly Castro for informative feedback, and Mike Fox for assistance with community analyses. Research permits were provided by the Autoridad Nacional del Ambiente de Panamá. This paper is the result of research funded by the National Oceanic and Atmospheric Administration’s National Centers for Coastal Ocean Science Competitive Research Program under award NA18NOS4780170 to A.H.A. and M.D.J. through the University of Florida. This is contribution 257 from the Coastal Hypoxia Research Program and 86 from the Smithsonian’s MarineGEO and Tennenbaum Marine Observatories Network.
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  • 69
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Brandt, P. D., Sturzenegger Varvayanis, S., Baas, T., Bolgioni, A. F., Alder, J., Petrie, K. A., Dominguez, I., Brown, A. M., Stayart, C. A., Singh, H., Van Wart, A., Chow, C. S., Mathur, A., Schreiber, B. M., Fruman, D. A., Bowden, B., Wiesen, C. A., Golightly, Y. M., Holmquist, C. E., Arneman, D., Hall, J. D., Hyman, L. E., Gould, K. L., Chalkley, R., Brennwald, P. J., Layton, R. L. A cross-institutional analysis of the effects of broadening trainee professional development on research productivity. Plos Biology, 19(7), (2021): e3000956, https://doi.org/10.1371/journal.pbio.3000956.
    Description: PhD-trained scientists are essential contributors to the workforce in diverse employment sectors that include academia, industry, government, and nonprofit organizations. Hence, best practices for training the future biomedical workforce are of national concern. Complementing coursework and laboratory research training, many institutions now offer professional training that enables career exploration and develops a broad set of skills critical to various career paths. The National Institutes of Health (NIH) funded academic institutions to design innovative programming to enable this professional development through a mechanism known as Broadening Experiences in Scientific Training (BEST). Programming at the NIH BEST awardee institutions included career panels, skill-building workshops, job search workshops, site visits, and internships. Because doctoral training is lengthy and requires focused attention on dissertation research, an initial concern was that students participating in additional complementary training activities might exhibit an increased time to degree or diminished research productivity. Metrics were analyzed from 10 NIH BEST awardee institutions to address this concern, using time to degree and publication records as measures of efficiency and productivity. Comparing doctoral students who participated to those who did not, results revealed that across these diverse academic institutions, there were no differences in time to degree or manuscript output. Our findings support the policy that doctoral students should participate in career and professional development opportunities that are intended to prepare them for a variety of diverse and important careers in the workforce.
    Description: Funding sources included the Common Fund NIH Director’s Biomedical Research Workforce Innovation Broadening Experiences in Scientific Training (BEST) Award. The following institutional NIH BEST awards (alphabetical by institution) included: DP7OD020322 (Boston University; AFB, ID, BMS, LEH); DP7OD020316 (University of Chicago; CAS); DP7OD018425 (Cornell University; SSV); DP7OD018428 (Virginia Polytechnic Institute; AVW, BB); DP7OD020314 (Rutgers University; JA); DP7OD020315 (University of Rochester; TB); DP7OD018423 (Vanderbilt University; KAP, AMB, KLG, RC); DP7OD020321 (University of California, Irvine; HS, DAF); DP7OD020317 (University of North Carolina, Chapel Hill; PDB, PJB, RLL); DP7 OD018427 (Wayne State University; CSC, AM). National Institutes of Health (NIH) General Medical Sciences - Science of Science Policy Approach to Analyzing and Innovating the Biomedical Research Enterprise (SCISIPBIO) Award (GM-19-011) - 1R01GM140282-01 (University of North Carolina at Chapel Hill; RLL, PDB, PJB).
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  • 70
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Wu, T., Tivey, M. A., Tao, C., Zhang, J., Zhou, F., & Liu, Y. An intermittent detachment faulting system with a large sulfide deposit revealed by multi-scale magnetic surveys. Nature Communications, 12(1), (2021): 5642, https://doi.org/10.1038/s41467-021-25880-1.
    Description: Magmatic and tectonic processes can contribute to discontinuous crustal accretion and play an important role in hydrothermal circulation at ultraslow-spreading ridges, however, it is difficult to accurately describe the processes without an age framework to constrain crustal evolution. Here we report on a multi-scale magnetic survey that provides constraints on the fine-scale evolution of a detachment faulting system that hosts hydrothermal activity at 49.7°E on the Southwest Indian Ridge. Reconstruction of the multi-stage detachment faulting history shows a previous episode of detachment faulting took place 0.76~1.48 My BP, while the present fault has been active for the past ~0.33 My and is just in the prime of life. This fault sustains hydrothermal circulation that has the potential for developing a large sulfide deposit. High resolution multiscale magnetics allows us to constrain the relative balance between periods of detachment faulting and magmatism to better describe accretionary processes on an ultraslow spreading ridge.
    Description: This work was supported by National Key R&D Program of China under contract no. 2018YFC0309901, National Natural Science Foundation of China (41906065), National Key R&D Program of China (2017YFC0306803) and COMRA Major Project under contract no. DY135-S1-01-06 and no. DY135-S1-01-01.
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  • 71
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Li, S., Raychaudhuri, S., Lee, S. A., Brockmann, M. M., Wang, J., Kusick, G., Prater, C., Syed, S., Falahati, H., Ramos, R., Bartol, T. M., Hosy, E., & Watanabe, S. Asynchronous release sites align with NMDA receptors in mouse hippocampal synapses. Nature Communications, 12(1), (2021): 677, https://doi.org/10.1038/s41467-021-21004-x.
    Description: Neurotransmitter is released synchronously and asynchronously following an action potential. Our recent study indicates that the release sites of these two phases are segregated within an active zone, with asynchronous release sites enriched near the center in mouse hippocampal synapses. Here we demonstrate that synchronous and asynchronous release sites are aligned with AMPA receptor and NMDA receptor clusters, respectively. Computational simulations indicate that this spatial and temporal arrangement of release can lead to maximal membrane depolarization through AMPA receptors, alleviating the pore-blocking magnesium leading to greater activation of NMDA receptors. Together, these results suggest that release sites are likely organized to activate NMDA receptors efficiently.
    Description: e also thank the Marine Biological Laboratory and their Neurobiology course for supporting the initial set of experiments (course supported by National Institutes of Health grant R25NS063307). S.W. and this work were supported by start-up funds from the Johns Hopkins University School of Medicine, Johns Hopkins Discovery funds, and the National Science Foundation (1727260), the National Institutes of Health (1DP2 NS111133-01 and 1R01 NS105810-01A1) awarded to S.W. S.W. is an Alfred P. Sloan fellow, McKnight Foundation Scholar, and Klingenstein and Simons Foundation scholar. G.K. was supported by a grant from the National Institutes of Health to the Biochemistry, Cellular and Molecular Biology Program of the Johns Hopkins University School of Medicine (T32 GM007445) and is a National Science Foundation Graduate Research Fellow (2016217537). E.H. and T.M.B. are supported by CRCNS-NIH-ANR grant AMPAR-T. The EM ICE high-pressure freezer was purchased partly with funds from an equipment grant from the National Institutes of Health (S10RR026445) awarded to Scot C Kuo.
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  • 72
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Sil, S., Gangopadhyay, A., Gawarkiewicz, G., & Pramanik, S. Shifting seasonality of cyclones and western boundary current interactions in Bay of Bengal as observed during Amphan and Fani. Scientific Reports, 11(1), (2021): 22052 https://doi.org/10.1038/s41598-021-01607-6.
    Description: In recent years, the seasonal patterns of Tropical Cyclones (TC) in the Bay of Bengal have been shifting. While tropical depressions have been common in March–May (spring), they typically have been relatively weaker than the TCs during October–December. Here we show that the spatial pattern of recent warming trends during the last two decades in the southwestern Bay has allowed for stronger springtime pre-monsoon cyclones such as Amphan (May 2020, Super Cyclone) and Fani (April–May 2019, Extremely Severe Cyclone). The tracks of the pre-monsoon cyclones shifted westward, concurrent with an increasing rate of warming. This shift allowed both Fani and Amphan tracks to cross the northeastward warm Western Boundary Current (WBC) and associated warm anti-cyclonic eddies, while the weaker Viyaru (April 2013, Cyclonic Storm) did not interact with the WBC. A quantitative model linking the available along-track heat potential to cyclone’s intensity is developed to understand the impact of the WBC on cyclone intensification. The influence of the warming WBC and associated anti-cyclonic eddies will likely result in much stronger springtime TCs becoming relatively common in the future.
    Description: The authors gratefully acknowledge the financial and infrastructural support from the Indian Institute of Technology Bhubaneswar to carry out this research. SS acknowledges the financial assistance from Science and Engineering Research Board (SERB), Government of India (Grant No. CRG/2019/005842). All the figures are prepared using MATLAB. AG and SS appreciate the support of SERB's VAJRA Faculty Scheme (VJR/2018/000108) for the initiation of this collaborative work between SMAST and IITBBS. AG also acknowledges partial support from NSF (OCE 1851242) in completing this manuscript. GG was supported by a Grant from the Office of Naval Research as part of the Task Force Ocean initiative.
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  • 73
    Publication Date: 2022-05-27
    Description: Author Posting. © American Meteorological Society, 2021. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Bulletin of the American Meteorological Society 102(10), (2021): E1897–E1935, https://doi.org/10.1175/BAMS-D-19-0316.1.
    Description: Life on Earth vitally depends on the availability of water. Human pressure on freshwater resources is increasing, as is human exposure to weather-related extremes (droughts, storms, floods) caused by climate change. Understanding these changes is pivotal for developing mitigation and adaptation strategies. The Global Climate Observing System (GCOS) defines a suite of essential climate variables (ECVs), many related to the water cycle, required to systematically monitor Earth’s climate system. Since long-term observations of these ECVs are derived from different observation techniques, platforms, instruments, and retrieval algorithms, they often lack the accuracy, completeness, and resolution, to consistently characterize water cycle variability at multiple spatial and temporal scales. Here, we review the capability of ground-based and remotely sensed observations of water cycle ECVs to consistently observe the hydrological cycle. We evaluate the relevant land, atmosphere, and ocean water storages and the fluxes between them, including anthropogenic water use. Particularly, we assess how well they close on multiple temporal and spatial scales. On this basis, we discuss gaps in observation systems and formulate guidelines for future water cycle observation strategies. We conclude that, while long-term water cycle monitoring has greatly advanced in the past, many observational gaps still need to be overcome to close the water budget and enable a comprehensive and consistent assessment across scales. Trends in water cycle components can only be observed with great uncertainty, mainly due to insufficient length and homogeneity. An advanced closure of the water cycle requires improved model–data synthesis capabilities, particularly at regional to local scales.
    Description: WD acknowledges ESA’s QA4EO (ISMN) and CCI Soil Moisture projects. WD, CRV, AG, and KL acknowledge the G3P project, which has received funding from the European Union’s Horizon 2020 research and innovation programme under Grant Agreement 870353. MIH and MS acknowledge ESA’s CCI Water Vapour project. MS and RH acknowledges the support by the EUMETSAT member states through CM SAF. DGM acknowledges support from the European Research Council (ERC) under Grant Agreement 715254 (DRY–2–DRY). Part of this research was carried out at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration (80NM0018D0004).
    Description: 2022-04-01
    Keywords: Hydrologic cycle ; Satellite observations ; Surface fluxes ; Surface observations ; Water masses/storage ; Water budget/balance
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  • 74
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Lenz, P. H., Roncalli, V., Cieslak, M. C., Tarrant, A. M., Castelfranco, A. M., & Hartline, D. K. Diapause vs. reproductive programs: transcriptional phenotypes in a keystone copepod. Communications Biology, 4(1), (2021): 426, https://doi.org/10.1038/s42003-021-01946-0.
    Description: Many arthropods undergo a seasonal dormancy termed “diapause” to optimize timing of reproduction in highly seasonal environments. In the North Atlantic, the copepod Calanus finmarchicus completes one to three generations annually with some individuals maturing into adults, while others interrupt their development to enter diapause. It is unknown which, why and when individuals enter the diapause program. Transcriptomic data from copepods on known programs were analyzed using dimensionality reduction of gene expression and functional analyses to identify program-specific genes and biological processes. These analyses elucidated physiological differences and established protocols that distinguish between programs. Differences in gene expression were associated with maturation of individuals on the reproductive program, while those on the diapause program showed little change over time. Only two of six filters effectively separated copepods by developmental program. The first one included all genes annotated to RNA metabolism and this was confirmed using differential gene expression analysis. The second filter identified 54 differentially expressed genes that were consistently up-regulated in individuals on the diapause program in comparison with those on the reproductive program. Annotated to oogenesis, RNA metabolism and fatty acid biosynthesis, these genes are both indicators for diapause preparation and good candidates for functional studies.
    Description: This work was supported by National Science Foundation Grants (NSF) OCE-1459235 and OCE-1756767 to P.H.L., D.K.H. and AE Christie and OPP-1746087 to A.M.T.
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  • 75
    Publication Date: 2022-05-27
    Description: Author Posting. © American Meteorological Society, 2021. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 51(2), (2021): 457–474, https://doi.org/10.1175/JPO-D-20-0088.1.
    Description: The meridional shift of the Kuroshio Extension (KE) front and changes in the formation of the North Pacific Subtropical Mode Water (STMW) during 1979–2018 are reported. The surface-to-subsurface structure of the KE front averaged over 142°–165°E has shifted poleward at a rate of ~0.23° ± 0.16° decade−1. The shift was caused mainly by the poleward shift of the downstream KE front (153°–165°E, ~0.41° ± 0.29° decade−1) and barely by the upstream KE front (142°–153°E). The long-term shift trend of the KE front showed two distinct behaviors before and after 2002. Before 2002, the surface KE front moved northward with a faster rate than the subsurface. After 2002, the surface KE front showed no obvious trend, but the subsurface KE front continued to move northward. The ventilation zone of the STMW, defined by the area between the 16° and 18°C isotherms or between the 25 and 25.5 kg m−3 isopycnals, contracted and displaced northward with a shoaling of the mixed layer depth hm before 2002 when the KE front moved northward. The STMW subduction rate was reduced by 0.76 Sv (63%; 1 Sv ≡ = 106 m3 s−1) during 1979–2018, most of which occurred before 2002. Of the three components affecting the total subduction rate, the temporal induction (−∂hm/∂t) was dominant accounting for 91% of the rate reduction, while the vertical pumping (−wmb) amounted to 8% and the lateral induction (−umb ⋅ ∇hm) was insignificant. The reduced temporal induction was attributed to both the contracted ventilation zone and the shallowed hm that were incurred by the poleward shift of KE front.
    Description: Xiaopei Lin is supported by the National Natural Science Foundation of China (41925025 and 92058203) and China’s national key research and development projects (2016YFA0601803). Baolan Wu is supported by the China Scholarship Council (201806330010). Lisan Yu thanks NOAA for support for her study on climate change and variability.
    Keywords: Boundary currents ; Decadal variability ; Fronts ; Water masses/storage
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  • 76
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Eide, M., Zhang, X., Karlsen, O. A., Goldstone, J., Stegeman, J., Jonassen, I., & Goksoyr, A. The chemical defensome of five model teleost fish. Scientific Reports, 11(1), (2021): 10546, https://doi.org/10.1038/s41598-021-89948-0.
    Description: How an organism copes with chemicals is largely determined by the genes and proteins that collectively function to defend against, detoxify and eliminate chemical stressors. This integrative network includes receptors and transcription factors, biotransformation enzymes, transporters, antioxidants, and metal- and heat-responsive genes, and is collectively known as the chemical defensome. Teleost fish is the largest group of vertebrate species and can provide valuable insights into the evolution and functional diversity of defensome genes. We have previously shown that the xenosensing pregnane x receptor (pxr, nr1i2) is lost in many teleost species, including Atlantic cod (Gadus morhua) and three-spined stickleback (Gasterosteus aculeatus), but it is not known if compensatory mechanisms or signaling pathways have evolved in its absence. In this study, we compared the genes comprising the chemical defensome of five fish species that span the teleosteii evolutionary branch often used as model species in toxicological studies and environmental monitoring programs: zebrafish (Danio rerio), medaka (Oryzias latipes), Atlantic killifish (Fundulus heteroclitus), Atlantic cod, and three-spined stickleback. Genome mining revealed evolved differences in the number and composition of defensome genes that can have implication for how these species sense and respond to environmental pollutants, but we did not observe any candidates of compensatory mechanisms or pathways in cod and stickleback in the absence of pxr. The results indicate that knowledge regarding the diversity and function of the defensome will be important for toxicological testing and risk assessment studies.
    Description: The work was supported by the Norwegian Research Council as part of the iCOD and iCOD 2.0 projects (Grant Nos. 192441/I30 and 244654/E40), and the dCod 1.0 project (Grant No. 248840) which is part of Centre for Digital Life Norway. The American collaborators were funded by the National Institute of Health (USA) NIH P42ES007381 (Boston University Superfund Center to JJS and JVG), NIH R21HD073805 (JVG) and NHI R01ES029917 (JVG) grants. The Ocean Outlook exchange program funded the trans-Atlantic collaboration.
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  • 77
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Lapointe, B. E., Brewton, R. A., Herren, L. W., Wang, M., Hu, C., McGillicuddy, D. J., Lindell, S., Hernandez, F. J., & Morton, P. L. Nutrient content and stoichiometry of pelagic Sargassum reflects increasing nitrogen availability in the Atlantic Basin. Nature Communications, 12(1), (2021): 3060, https://doi.org/10.1038/s41467-021-23135-7.
    Description: The pelagic brown macroalgae Sargassum spp. have grown for centuries in oligotrophic waters of the North Atlantic Ocean supported by natural nutrient sources, such as excretions from associated fishes and invertebrates, upwelling, and N2 fixation. Using a unique historical baseline, we show that since the 1980s the tissue %N of Sargassum spp. has increased by 35%, while %P has decreased by 44%, resulting in a 111% increase in the N:P ratio (13:1 to 28:1) and increased P limitation. The highest %N and δ15N values occurred in coastal waters influenced by N-rich terrestrial runoff, while lower C:N and C:P ratios occurred in winter and spring during peak river discharges. These findings suggest that increased N availability is supporting blooms of Sargassum and turning a critical nursery habitat into harmful algal blooms with catastrophic impacts on coastal ecosystems, economies, and human health.
    Description: This work was funded by the US NASA Ocean Biology and Biogeochemistry Program (80NSSC20M0264, NNX16AR74G) and Ecological Forecast Program (NNX17AF57G), NOAA RESTORE Science Program (NA17NOS4510099), National Science Foundation (NSF-OCE 85–15492 and OCE 88–12055), “Save Our Seas” Specialty License Plate funds, granted through the Harbor Branch Oceanographic Institute Foundation, Ft. Pierce, FL, and a Red Wright Fellowship from the Bermuda Biological Station. A portion of this work was performed at the National High Magnetic Field Laboratory, which is supported by National Science Foundation Cooperative Agreement No. DMR-1644779 and the State of Florida. D.J.M. gratefully acknowledges the Holger W. Jannasch and Columbus O’Donnell Iselin Shared Chairs for Excellence in Oceanography, as well as support from the Mill Reef Fund.
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  • 78
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in McPherson, M. L., Finger, D. J., I., Houskeeper, H. F., Bell, T. W., Carr, M. H., Rogers-Bennett, L., & Kudela, R. M. Large-scale shift in the structure of a kelp forest ecosystem co-occurs with an epizootic and marine heatwave. Communications Biology, 4(1), (2021): 298, https://doi.org/10.1038/s42003-021-01827-6.
    Description: Climate change is responsible for increased frequency, intensity, and duration of extreme events, such as marine heatwaves (MHWs). Within eastern boundary current systems, MHWs have profound impacts on temperature-nutrient dynamics that drive primary productivity. Bull kelp (Nereocystis luetkeana) forests, a vital nearshore habitat, experienced unprecedented losses along 350 km of coastline in northern California beginning in 2014 and continuing through 2019. These losses have had devastating consequences to northern California communities, economies, and fisheries. Using a suite of in situ and satellite-derived data, we demonstrate that the abrupt ecosystem shift initiated by a multi-year MHW was preceded by declines in keystone predator population densities. We show strong evidence that northern California kelp forests, while temporally dynamic, were historically resilient to fluctuating environmental conditions, even in the absence of key top predators, but that a series of coupled environmental and biological shifts between 2014 and 2016 resulted in the formation of a persistent, altered ecosystem state with low primary productivity. Based on our findings, we recommend the implementation of ecosystem-based and adaptive management strategies, such as (1) monitoring the status of key ecosystem attributes: kelp distribution and abundance, and densities of sea urchins and their predators, (2) developing management responses to threshold levels of these attributes, and (3) creating quantitative restoration suitability indices for informing kelp restoration efforts.
    Description: M.H.C. received support from the National Science Foundation (OCE‐1538582).
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  • 79
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Lai, C.-Y., Stevens, L. A., Chase, D. L., Creyts, T. T., Behn, M. D., Das, S. B., & Stone, H. A. Hydraulic transmissivity inferred from ice-sheet relaxation following Greenland supraglacial lake drainages. Nature Communications, 12(1), (2021): 3955, https://doi.org/10.1038/s41467-021-24186-6.
    Description: Surface meltwater reaching the base of the Greenland Ice Sheet transits through drainage networks, modulating the flow of the ice sheet. Dye and gas-tracing studies conducted in the western margin sector of the ice sheet have directly observed drainage efficiency to evolve seasonally along the drainage pathway. However, the local evolution of drainage systems further inland, where ice thicknesses exceed 1000 m, remains largely unknown. Here, we infer drainage system transmissivity based on surface uplift relaxation following rapid lake drainage events. Combining field observations of five lake drainage events with a mathematical model and laboratory experiments, we show that the surface uplift decreases exponentially with time, as the water in the blister formed beneath the drained lake permeates through the subglacial drainage system. This deflation obeys a universal relaxation law with a timescale that reveals hydraulic transmissivity and indicates a two-order-of-magnitude increase in subglacial transmissivity (from 0.8 ± 0.3 mm3 to 215 ± 90.2 mm3) as the melt season progresses, suggesting significant changes in basal hydrology beneath the lakes driven by seasonal meltwater input.
    Description: C.-Y.L. and L.A.S thank Lamont-Doherty Earth Observatory for funding through the Lamont Postdoctoral Fellowships. D.L.C acknowledges support from the National Science Foundation (NSF) Graduate Research Fellowship. T.T.C. was supported by NSF’s Office of Polar Programs (NSF-OPP) through OPP-1643970, the National Aeronautics and Space Administration (NASA) through NNX16AJ95G, and a grant from the Vetlesen Foundation. S.B.D. and M.D.B. acknowledge funding from NSF-OPP and NASA’s Cryospheric Sciences Program through OPP-1838410, ARC-1023364, ARC-0520077, and NNX10AI30G. H.A.S. thanks the High Meadows Environmental Institute and the Carbon Mitigation Initiative at Princeton University. This publication was supported by the Princeton University Library Open Access Fund.
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  • 80
    Publication Date: 2022-05-27
    Description: Author Posting. © American Meteorological Society, 2021. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 51(8),(2021): 2463–2482, https://doi.org/10.1175/JPO-D-20-0291.1.
    Description: This paper presents analyses of drifters with drogues at different depths—1, 10, 30, and 50 m—that were deployed in the Mediterranean Sea to investigate frontal subduction and upwelling. Drifter trajectories were used to estimate divergence, vorticity, vertical velocity, and finite-size Lyapunov exponents (FTLEs) and to investigate the balance of terms in the vorticity equation. The divergence and vorticity are O(f) and change sign along trajectories. Vertical velocity is O(1 mm s−1), increases with depth, indicates predominant upwelling with isolated downwelling events, and sometimes changes sign between 1 and 50 m. Vortex stretching is one of the significant terms, but not the only one, in the vorticity balance. Two-dimensional FTLEs are 2 × 10−5 s−1 after 1 day, 2 times as large as in a 400-m-resolution numerical model. Three-dimensional FTLEs are 50% larger than 2D FTLEs and are dominated by the vertical shear of horizontal velocity. Bootstrapping suggests uncertainty levels of ~10% of the time-mean absolute values for divergence and vorticity. Analysis of simulated drifters in a model suggests that drifter-based estimates of divergence and vorticity are close to the Eulerian model estimates, except when drifters get aligned into long filaments. Drifter-based vertical velocity is close to the Eulerian model estimates at 1 m but differs at deeper depths. The errors in the vertical velocity are largely due to the lateral separation between drifters at different depths and are partially due to only measuring at four depths. Overall, this paper demonstrates how drifters, heretofore restricted to 2D near-surface observations, can be used to learn about 3D flow properties throughout the upper layer of the water column.
    Description: Authors Rypina and Pratt were supported by U.S. Office of Naval Research (ONR) Grant N000141812417. Author Getscher acknowledges support from the U.S. Navy Civilian Institution Office with the MIT–WHOI Joint Program. Author Mourre acknowledges support from ONR Grant N00014-16-1-3130. We also thank Eugenio Cutolo for the initial technical support in the implementation of the ultra-high-resolution WMOP simulation. CALYPSO is a Departmental Research Initiative funded by the ONR.
    Description: 2022-01-16
    Keywords: Convergence/divergence ; Fronts ; Nonlinear dynamics ; Small scale processes ; Trajectories ; Upwelling/downwelling ; Vertical motion
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  • 81
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Lin, Y., Moreno, C., Marchetti, A., Ducklow, H., Schofield, O., Delage, E., Meredith, M., Li, Z., Eveillard, D., Chaffron, S., & Cassar, N. Decline in plankton diversity and carbon flux with reduced sea ice extent along the Western Antarctic Peninsula. Nature Communications, 12(1), (2021): 4948, https://doi.org/10.1038/s41467-021-25235-w.
    Description: Since the middle of the past century, the Western Antarctic Peninsula has warmed rapidly with a significant loss of sea ice but the impacts on plankton biodiversity and carbon cycling remain an open question. Here, using a 5-year dataset of eukaryotic plankton DNA metabarcoding, we assess changes in biodiversity and net community production in this region. Our results show that sea-ice extent is a dominant factor influencing eukaryotic plankton community composition, biodiversity, and net community production. Species richness and evenness decline with an increase in sea surface temperature (SST). In regions with low SST and shallow mixed layers, the community was dominated by a diverse assemblage of diatoms and dinoflagellates. Conversely, less diverse plankton assemblages were observed in waters with higher SST and/or deep mixed layers when sea ice extent was lower. A genetic programming machine-learning model explained up to 80% of the net community production variability at the Western Antarctic Peninsula. Among the biological explanatory variables, the sea-ice environment associated plankton assemblage is the best predictor of net community production. We conclude that eukaryotic plankton diversity and carbon cycling at the Western Antarctic Peninsula are strongly linked to sea-ice conditions.
    Description: This work is supported by NSF OPP-1643534 to N.C., NSF OPP-1341479 to A.M., and NSF PLR-1440435 to H.D. and O.S. (Palmer LTER).
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  • 82
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Keikhosravi, A., Shribak, M., Conklin, M. W., Liu, Y., Li, B., Loeffler, A., Levenson, R. M., & Eliceiri, K. W. Real-time polarization microscopy of fibrillar collagen in histopathology. Scientific Reports, 11(1), (2021): 19063, https://doi.org/10.1038/s41598-021-98600-w.
    Description: Over the past two decades, fibrillar collagen reorganization parameters such as the amount of collagen deposition, fiber angle and alignment have been widely explored in numerous studies. These parameters are now widely accepted as stromal biomarkers and linked to disease progression and survival time in several cancer types. Despite all these advances, there has not been a significant effort to make it possible for clinicians to explore these biomarkers without adding steps to the clinical workflow or by requiring high-cost imaging systems. In this paper, we evaluate previously described polychromatic polarization microscope (PPM) to visualize collagen fibers with an optically generated color representation of fiber orientation and alignment when inspecting the sample by a regular microscope with minor modifications. This system does not require stained slides, but is compatible with histological stains such as H&E. Consequently, it can be easily accommodated as part of regular pathology review of tissue slides, while providing clinically useful insight into stromal composition.
    Description: This work was supported by NIH R01 CA238191 (KWE), NIH P41GM135019 (KWE), NIH R01 GM101701 (MS), funding from the Morgridge Institute for Research (KWE), the Semiconductor Research Corporation (SRC) (KWE), and the William T. Golden Endowment (MS).
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  • 83
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Brun, N. R., Panlilio, J. M., Zhang, K., Zhao, Y., Ivashkin, E., Stegeman, J. J., & Goldstone, J. Developmental exposure to non-dioxin-like polychlorinated biphenyls promotes sensory deficits and disrupts dopaminergic and GABAergic signaling in zebrafish. Communications Biology, 4(1), (2021): 1129, https://doi.org/10.1038/s42003-021-02626-9.
    Description: The most abundant polychlorinated biphenyl (PCB) congeners found in the environment and in humans are neurotoxic. This is of particular concern for early life stages because the exposure of the more vulnerable developing nervous system to neurotoxic chemicals can result in neurobehavioral disorders. In this study, we uncover currently unknown links between PCB target mechanisms and neurobehavioral deficits using zebrafish as a vertebrate model. We investigated the effects of the abundant non-dioxin-like (NDL) congener PCB153 on neuronal morphology and synaptic transmission linked to the proper execution of a sensorimotor response. Zebrafish that were exposed during development to concentrations similar to those found in human cord blood and PCB contaminated sites showed a delay in startle response. Morphological and biochemical data demonstrate that even though PCB153-induced swelling of afferent sensory neurons, the disruption of dopaminergic and GABAergic signaling appears to contribute to PCB-induced motor deficits. A similar delay was observed for other NDL congeners but not for the potent dioxin-like congener PCB126. The effects on important and broadly conserved signaling mechanisms in vertebrates suggest that NDL PCBs may contribute to neurodevelopmental abnormalities in humans and increased selection pressures in vertebrate wildlife.
    Description: This work was supported by the Swiss National Science Foundation P2EZP2_165200 (NRB), the Boston University Superfund Research Program NIH 5P42ES007381 (J.J.S. and J.V.G.), the Woods Hole Center for Oceans and Human Health (NIH: P01ES021923 and P01ES028938; NSF: OCE-1314642 and OCE-1840381) (N.R.B., J.M.P., and J.J.S.), and the National Natural Science Foundation of China 22006099 (K.Z. and Y.Z.) and the Shanghai Pujiang Program 19PJ1404900 (K.Z. and Y.Z.).
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  • 84
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Mayorova, T. D., Hammar, K., Jung, J. H., Aronova, M. A., Zhang, G., Winters, C. A., Reese, T. S., & Smith, C. L. Placozoan fiber cells: mediators of innate immunity and participants in wound healing. Scientific Reports, 11(1), (2021): 23343, https://doi.org/10.1038/s41598-021-02735-9.
    Description: Placozoa is a phylum of non-bilaterian marine animals. These small, flat organisms adhere to the substrate via their densely ciliated ventral epithelium, which mediates mucociliary locomotion and nutrient uptake. They have only six morphological cell types, including one, fiber cells, for which functional data is lacking. Fiber cells are non-epithelial cells with multiple processes. We used electron and light microscopic approaches to unravel the roles of fiber cells in Trichoplax adhaerens, a representative member of the phylum. Three-dimensional reconstructions of serial sections of Trichoplax showed that each fiber cell is in contact with several other cells. Examination of fiber cells in thin sections and observations of live dissociated fiber cells demonstrated that they phagocytose cell debris and bacteria. In situ hybridization confirmed that fiber cells express genes involved in phagocytic activity. Fiber cells also are involved in wound healing as evidenced from microsurgery experiments. Based on these observations we conclude that fiber cells are multi-purpose macrophage-like cells. Macrophage-like cells have been described in Porifera, Ctenophora, and Cnidaria and are widespread among Bilateria, but our study is the first to show that Placozoa possesses this cell type. The phylogenetic distribution of macrophage-like cells suggests that they appeared early in metazoan evolution.
    Description: Open Access funding provided by the National Institutes of Health (NIH).
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  • 85
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Brun, N. R., Salanga, M. C., Mora-Zamorano, F. X., Lamb, D. C., Goldstone, J. V., & Stegeman, J. J. Orphan cytochrome P450 20a1 CRISPR/Cas9 mutants and neurobehavioral phenotypes in zebrafish. Scientific Reports, 11(1), (2021): 23892, https://doi.org/10.1038/s41598-021-03068-3.
    Description: Orphan cytochrome P450 (CYP) enzymes are those for which biological substrates and function(s) are unknown. Cytochrome P450 20A1 (CYP20A1) is the last human orphan P450 enzyme, and orthologs occur as single genes in every vertebrate genome sequenced to date. The occurrence of high levels of CYP20A1 transcripts in human substantia nigra and hippocampus and abundant maternal transcripts in zebrafish eggs strongly suggest roles both in the brain and during early embryonic development. Patients with chromosome 2 microdeletions including CYP20A1 show hyperactivity and bouts of anxiety, among other conditions. Here, we created zebrafish cyp20a1 mutants using CRISPR/Cas9, providing vertebrate models with which to study the role of CYP20A1 in behavior and other neurodevelopmental functions. The homozygous cyp20a1 null mutants exhibited significant behavioral differences from wild-type zebrafish, both in larval and adult animals. Larval cyp20a1-/- mutants exhibited a strong increase in light-simulated movement (i.e., light–dark assay), which was interpreted as hyperactivity. Further, the larvae exhibited mild hypoactivity during the adaptation period of the optomotor assays. Adult cyp20a1 null fish showed a pronounced delay in adapting to new environments, which is consistent with an anxiety paradigm. Taken together with our earlier morpholino cyp20a1 knockdown results, the results described herein suggest that the orphan CYP20A1 has a neurophysiological role.
    Description: These studies were supported in part by the Boston University Superfund Research Program NIH 5P42ES007381 (MCS, NRB, FXM, JVG, JJS), the Woods Hole Center for Oceans and Human Health (NIH: P01ES021923 and P01ES028938; NSF: OCE-1314642 and OCE-1840381; NRB and JJS), and EBI/EMBL Medakatox NIEHS R01ES029917 (JVG). DCL was funded by a UK-US Fulbright Scholarship.
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  • 86
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Beman, J. M., Vargas, S. M., Wilson, J. M., Perez-Coronel, E., Karolewski, J. S., Vazquez, S., Yu, A., Cairo, A. E., White, M. E., Koester, I., Aluwihare, L. I., & Wankel, S. D. Substantial oxygen consumption by aerobic nitrite oxidation in oceanic oxygen minimum zones. Nature Communications, 12(1), (2021): 7043, https://doi.org/10.1038/s41467-021-27381-7.
    Description: Oceanic oxygen minimum zones (OMZs) are globally significant sites of biogeochemical cycling where microorganisms deplete dissolved oxygen (DO) to concentrations 〈20 µM. Amid intense competition for DO in these metabolically challenging environments, aerobic nitrite oxidation may consume significant amounts of DO and help maintain low DO concentrations, but this remains unquantified. Using parallel measurements of oxygen consumption rates and 15N-nitrite oxidation rates applied to both water column profiles and oxygen manipulation experiments, we show that the contribution of nitrite oxidation to overall DO consumption systematically increases as DO declines below 2 µM. Nitrite oxidation can account for all DO consumption only under DO concentrations 〈393 nM found in and below the secondary chlorophyll maximum. These patterns are consistent across sampling stations and experiments, reflecting coupling between nitrate reduction and nitrite-oxidizing Nitrospina with high oxygen affinity (based on isotopic and omic data). Collectively our results demonstrate that nitrite oxidation plays a pivotal role in the maintenance and biogeochemical dynamics of OMZs.
    Description: This work was supported by NSF CAREER Grant OCE-1555375 to J.M.B. Metagenome sequencing was supported by the UCMEXUS-CONACyT Collaborative Grants Program (joint awards to J.M.B. and José García Maldonado).
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  • 87
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Remple, K. L., Silbiger, N. J., Quinlan, Z. A., Fox, M. D., Kelly, L. W., Donahue, M. J., & Nelson, C. E. Coral reef biofilm bacterial diversity and successional trajectories are structured by reef benthic organisms and shift under chronic nutrient enrichment. Npj Biofilms and Microbiomes, 7(1), (2021): 84, https://doi.org/10.1038/s41522-021-00252-1.
    Description: Work on marine biofilms has primarily focused on host-associated habitats for their roles in larval recruitment and disease dynamics; little is known about the factors regulating the composition of reef environmental biofilms. To contrast the roles of succession, benthic communities and nutrients in structuring marine biofilms, we surveyed bacteria communities in biofilms through a six-week succession in aquaria containing macroalgae, coral, or reef sand factorially crossed with three levels of continuous nutrient enrichment. Our findings demonstrate how biofilm successional trajectories diverge from temporal dynamics of the bacterioplankton and how biofilms are structured by the surrounding benthic organisms and nutrient enrichment. We identify a suite of biofilm-associated bacteria linked with the orthogonal influences of corals, algae and nutrients and distinct from the overlying water. Our results provide a comprehensive characterization of marine biofilm successional dynamics and contextualize the impact of widespread changes in reef community composition and nutrient pollution on biofilm community structure.
    Description: This work was supported through grants from the National Science Foundation for Biological Oceanography (1923877 to C.E.N. and M.J.D., 1949033 to C.E.N. and 2118687 to L.W.K., and 1924281 to N.J.S.) and the National Fish and Wildlife Foundation (grant no. 44447 to C.E.N.). This paper is funded in part by the National Oceanic and Atmospheric Administration, Project A/AS-1, which is sponsored by the University of Hawaii Sea Grant College Program, SOEST, under Institutional Grant No. NA18OAR4170076 from NOAA Office of Sea Grant, Department of Commerce. This is CSUN marine biology contribution #365, UH Sea Grant contribution UNIHI-SEAGRANT-JC-21-06, and UH SOEST contribution 11435.
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  • 88
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in James, B. D., Kimmins, K. M., Nguyen, M.-T., Lausch, A. J., & Sone, E. D. Attachment of zebra and quagga mussel adhesive plaques to diverse substrates. Scientific Reports, 11(1), (2021): 23998, https://doi.org/10.1038/s41598-021-03227-6.
    Description: Like marine mussels, freshwater zebra and quagga mussels adhere via the byssus, a proteinaceous attachment apparatus. Attachment to various surfaces allows these invasive mussels to rapidly spread, however the adhesion mechanism is not fully understood. While marine mussel adhesion mechanics has been studied at the individual byssal-strand level, freshwater mussel adhesion has only been characterized through whole-mussel detachment, without direct interspecies comparisons on different substrates. Here, adhesive strength of individual quagga and zebra mussel byssal plaques were measured on smooth substrates with varying hydrophobicity—glass, PVC, and PDMS. With increased hydrophobicity of substrates, adhesive failures occurred more frequently, and mussel adhesion strength decreased. A new failure mode termed 'footprint failure' was identified, where failure appeared to be adhesive macroscopically, but a microscopic residue remained on the surface. Zebra mussels adhered stronger and more frequently on PDMS than quagga mussels. While their adhesion strengths were similar on PVC, there were differences in the failure mode and the plaque-substrate interface ultrastructure. Comparisons with previous marine mussel studies demonstrated that freshwater mussels adhere with comparable strength despite known differences in protein composition. An improved understanding of freshwater mussel adhesion mechanics may help explain spreading dynamics and will be important in developing effective antifouling surfaces.
    Description: This work was supported by Discovery grant (#342455) to EDS from the National Sciences and Engineering Research Council (NSERC) of Canada (RGPIN-2019-06210).
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  • 89
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Johnson, M. D., Swaminathan, S. D., Nixon, E. N., Paul, V. J., & Altieri, A. H. Differential susceptibility of reef-building corals to deoxygenation reveals remarkable hypoxia tolerance. Scientific Reports, 11(1), (2021): 23168, https://doi.org/10.1038/s41598-021-01078-9.
    Description: Ocean deoxygenation threatens the persistence of coastal ecosystems worldwide. Despite an increasing awareness that coastal deoxygenation impacts tropical habitats, there remains a paucity of empirical data on the effects of oxygen limitation on reef-building corals. To address this knowledge gap, we conducted laboratory experiments with ecologically important Caribbean corals Acropora cervicornis and Orbicella faveolata. We tested the effects of continuous exposure to conditions ranging from extreme deoxygenation to normoxia (~ 1.0 to 6.25 mg L−1 dissolved oxygen) on coral bleaching, photophysiology, and survival. Coral species demonstrated markedly different temporal resistance to deoxygenation, and within a species there were minimal genotype-specific treatment effects. Acropora cervicornis suffered tissue loss and mortality within a day of exposure to severe deoxygenation (~ 1.0 mg L−1), whereas O. faveolata remained unaffected after 11 days of continuous exposure to 1.0 mg L−1. Intermediate deoxygenation treatments (~ 2.25 mg L−1, ~ 4.25 mg L−1) elicited minimal responses in both species, indicating a low oxygen threshold for coral mortality and coral resilience to oxygen concentrations that are lethal for other marine organisms. These findings demonstrate the potential for variability in species-specific hypoxia thresholds, which has important implications for our ability to predict how coral reefs may be affected as ocean deoxygenation intensifies. With deoxygenation emerging as a critical threat to tropical habitats, there is an urgent need to incorporate deoxygenation into coral reef research, management, and action plans to facilitate better stewardship of coral reefs in an era of rapid environmental change.
    Description: This research was funded by an award from the National Oceanic and Atmospheric Administration’s National Centers for Coastal Ocean Science Competitive Research Program to AA, MJ, and VP (NA18NOS4780170) through the University of Florida. MJ was funded by postdoctoral fellow awards from the Smithsonian Institution's Marine Global Earth Observatory (MarineGEO), the Smithsonian Marine Station, and Woods Hole Oceanographic Institution. This material is based on work supported by the National Science Foundation Graduate Research Fellowship under Grant No. DGE-1842473. This is contribution 259 from the Coastal Hypoxia Research Program, 93 from the Smithsonian’s MarineGEO and Tennenbaum Marine Observatories Network, and 1167 from the Smithsonian Marine Station at Fort Pierce.
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  • 90
    Publication Date: 2022-05-27
    Description: Author Posting. © American Meteorological Society, 2021. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Bulletin of the American Meteorological Society 102(10), (2021): E1936–E1951, https://doi.org/10.1175/BAMS-D-20-0113.1.
    Description: In the Bay of Bengal, the warm, dry boreal spring concludes with the onset of the summer monsoon and accompanying southwesterly winds, heavy rains, and variable air–sea fluxes. Here, we summarize the 2018 monsoon onset using observations collected through the multinational Monsoon Intraseasonal Oscillations in the Bay of Bengal (MISO-BoB) program between the United States, India, and Sri Lanka. MISO-BoB aims to improve understanding of monsoon intraseasonal variability, and the 2018 field effort captured the coupled air–sea response during a transition from active-to-break conditions in the central BoB. The active phase of the ∼20-day research cruise was characterized by warm sea surface temperature (SST 〉 30°C), cold atmospheric outflows with intermittent heavy rainfall, and increasing winds (from 2 to 15 m s−1). Accumulated rainfall exceeded 200 mm with 90% of precipitation occurring during the first week. The following break period was both dry and clear, with persistent 10–12 m s−1 wind and evaporation of 0.2 mm h−1. The evolving environmental state included a deepening ocean mixed layer (from ∼20 to 50 m), cooling SST (by ∼1°C), and warming/drying of the lower to midtroposphere. Local atmospheric development was consistent with phasing of the large-scale intraseasonal oscillation. The upper ocean stores significant heat in the BoB, enough to maintain SST above 29°C despite cooling by surface fluxes and ocean mixing. Comparison with reanalysis indicates biases in air–sea fluxes, which may be related to overly cool prescribed SST. Resolution of such biases offers a path toward improved forecasting of transition periods in the monsoon.
    Description: This work was supported through the U.S. Office of Naval Research’s Departmental Research Initiative: Monsoon Intraseasonal Oscillations in the Bay of Bengal, the Indian Ministry of Earth Science’s Ocean Mixing and Monsoons Program, and the Sri Lankan National Aquatic Resources Research and Development Agency. We thank the Captain and crew of the R/V Thompson for their help in data collection. Surface atmospheric fields included fluxes were quality controlled and processed by the Boundary Layer Observations and Processes Team within the NOAA Physical Sciences Laboratory. Forecast analysis was completed by India Meteorological Department. Drone image was taken by Shreyas Kamat with annotations by Gualtiero Spiro Jaeger. We also recognize the numerous researchers who supported cruise- and land-based measurements. This work represents Lamont-Doherty Earth Observatory contribution number 8503, and PMEL contribution number 5193.
    Description: 2022-04-01
    Keywords: Atmosphere-ocean interaction ; Monsoons ; In situ atmospheric observations ; In situ oceanic observations
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  • 91
    Publication Date: 2022-05-27
    Description: This paper is not subject to U.S. copyright. The definitive version was published in Castellote, M., Mooney, A., Andrews, R., Deruiter, S., Lee, W.-J., Ferguson, M., & Wade, P. Beluga whale (Delphinapterus leucas) acoustic foraging behavior and applications for long term monitoring. Plos One, 16(11), (2021): e0260485, https://doi.org/10.1371/journal.pone.0260485.
    Description: Cook Inlet, Alaska, is home to an endangered and declining population of 279 belugas (Delphinapterus leucas). Recovery efforts highlight a paucity of basic ecological knowledge, impeding the correct assessment of threats and the development of recovery actions. In particular, information on diet and foraging habitat is very limited for this population. Passive acoustic monitoring has proven to be an efficient approach to monitor beluga distribution and seasonal occurrence. Identifying acoustic foraging behavior could help address the current gap in information on diet and foraging habitat. To address this conservation challenge, eight belugas from a comparative, healthy population in Bristol Bay, Alaska, were instrumented with a multi-sensor tag (DTAG), a satellite tag, and a stomach temperature transmitter in August 2014 and May 2016. DTAG deployments provided 129.6 hours of data including foraging and social behavioral states. A total of 68 echolocation click trains ending in terminal buzzes were identified during successful prey chasing and capture, as well as during social interactions. Of these, 37 click trains were successfully processed to measure inter-click intervals (ICI) and ICI trend in their buzzing section. Terminal buzzes with short ICI (minimum ICI 〈8.98 ms) and consistently decreasing ICI trend (ICI increment range 〈1.49 ms) were exclusively associated with feeding behavior. This dual metric was applied to acoustic data from one acoustic mooring within the Cook Inlet beluga critical habitat as an example of the application of detecting feeding in long-term passive acoustic monitoring data. This approach allowed description of the relationship between beluga presence, feeding occurrence, and the timing of spawning runs by different species of anadromous fish. Results reflected a clear preference for the Susitna River delta during eulachon (Thaleichthys pacificus), Chinook (Oncorhynchus tshawytscha), pink (Oncorhynchus gorbuscha), and coho (Oncorhynchus kisutch) salmon spawning run periods, with increased feeding occurrence at the peak of the Chinook and pink salmon runs.
    Description: Project funding was provided by Georgia Aquarium, the Marine Mammal Laboratory of the Alaska Fisheries Science Center (MML/AFSC). Tagging was funded by the NOAA Fisheries Office of Science and Technology’s Ocean Acoustics Program. DTAG data analysis was funded by the U.S. Marine Mammal Commission grant #16-239. Funding for collecting and analyzing Cook Inlet beluga acoustic data in Susitna Delta was provided by the National Marine Fisheries Service Section 6 Office to the Alaska Department of Fish and Game. This publication is partially funded by the Cooperative Institute for Climate, Ocean, and Ecosystem Studies (CICOES), University of Washington, under NOAA Cooperative Agreement NA15OAR4320063, Contribution No. 2021-1145.
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  • 92
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Tyne, R. L., Barry, P. H., Lawson, M., Byrne, D. J., Warr, O., Xie, H., Hillegonds, D. J., Formolo, M., Summers, Z. M., Skinner, B., Eiler, J. M., & Ballentine, C. J. Rapid microbial methanogenesis during CO2 storage in hydrocarbon reservoirs. Nature, 600(7890), (2021): 670-674, https://doi.org/10.1038/s41586-021-04153-3.
    Description: Carbon capture and storage (CCS) is a key technology to mitigate the environmental impact of carbon dioxide (CO2) emissions. An understanding of the potential trapping and storage mechanisms is required to provide confidence in safe and secure CO2 geological sequestration1,2. Depleted hydrocarbon reservoirs have substantial CO2 storage potential1,3, and numerous hydrocarbon reservoirs have undergone CO2 injection as a means of enhanced oil recovery (CO2-EOR), providing an opportunity to evaluate the (bio)geochemical behaviour of injected carbon. Here we present noble gas, stable isotope, clumped isotope and gene-sequencing analyses from a CO2-EOR project in the Olla Field (Louisiana, USA). We show that microbial methanogenesis converted as much as 13–19% of the injected CO2 to methane (CH4) and up to an additional 74% of CO2 was dissolved in the groundwater. We calculate an in situ microbial methanogenesis rate from within a natural system of 73–109 millimoles of CH4 per cubic metre (standard temperature and pressure) per year for the Olla Field. Similar geochemical trends in both injected and natural CO2 fields suggest that microbial methanogenesis may be an important subsurface sink of CO2 globally. For CO2 sequestration sites within the environmental window for microbial methanogenesis, conversion to CH4 should be considered in site selection.
    Description: R.L.T. was supported by a Natural Environment Research Council studentship (grant reference NE/L002612/1). C.J.B. and P.H.B. acknowledge A. Regberg and B. Meurer for their support of the project and help with sample collection. C.J.B. was part supported by an Earth4D CIFAR fellowship. P.H.B. was supported by NSF awards 1923915 and 2015789. O.W. was supported by Natural Sciences and Engineering Research Council of Canada Discovery and Accelerator grants awarded to the Sherwood Lollar research group and acknowledges B. Sherwood Lollar’s support for the project. Z.M.S. acknowledges J. Biddle and G. Christman for their help in generating the microbial data.
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  • 93
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Sauvage, J. F., Flinders, A., Spivack, A. J., Pockalny, R., Dunlea, A. G., Anderson, C. H., Smith, D. C., Murray, R. W., & D'Hondt, S. The contribution of water radiolysis to marine sedimentary life. Nature Communications, 12(1), (2021): 1297, https://doi.org/10.1038/s41467-021-21218-z.
    Description: Water radiolysis continuously produces H2 and oxidized chemicals in wet sediment and rock. Radiolytic H2 has been identified as the primary electron donor (food) for microorganisms in continental aquifers kilometers below Earth’s surface. Radiolytic products may also be significant for sustaining life in subseafloor sediment and subsurface environments of other planets. However, the extent to which most subsurface ecosystems rely on radiolytic products has been poorly constrained, due to incomplete understanding of radiolytic chemical yields in natural environments. Here we show that all common marine sediment types catalyse radiolytic H2 production, amplifying yields by up to 27X relative to pure water. In electron equivalents, the global rate of radiolytic H2 production in marine sediment appears to be 1-2% of the global organic flux to the seafloor. However, most organic matter is consumed at or near the seafloor, whereas radiolytic H2 is produced at all sediment depths. Comparison of radiolytic H2 consumption rates to organic oxidation rates suggests that water radiolysis is the principal source of biologically accessible energy for microbial communities in marine sediment older than a few million years. Where water permeates similarly catalytic material on other worlds, life may also be sustained by water radiolysis.
    Description: This project was funded by the US National Science Foundation (through grant NSF-OCE-1130735 and the Center for Deep Dark Energy Biosphere Investigations [C-DEBI; grant NSF-OCE-0939564]); the National Aeronautics and Space Administration (grant NNX12AD65G); the U.S. Science Support Program, IODP; and a Schlanger Ocean Drilling Fellowship to J.F.S. This is a contribution to the Deep Carbon Observatory (DCO). It is C-DEBI publication 553.
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  • 94
    Publication Date: 2022-05-27
    Description: Author Posting. © American Meteorological Society, 2021. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 51(1), (2021): 247-266, https://doi.org/10.1175/JPO-D-20-0098.1.
    Description: This study focuses on mechanisms of shelf valley bathymetry affecting the spread of riverine freshwater in the nearshore region. In the context of Changjiang River, a numerical model is used with different no-tide idealized configurations to simulate development of unforced river plumes over a sloping bottom, with and without a shelf valley off the estuary mouth. All simulated freshwater plumes are surface-trapped with continuously growing bulges near the estuary mouth and narrow coastal currents downstream. The simulations indicate that a shelf valley tends to compress the bulge along the direction of the valley long axis and modify the incident angle of the bulge flow impinging toward the coast, which then affects the strength of the coastal current. The bulge compression results from geostrophic adjustment and isobath-following tendency of the depth-averaged flow in the bulge region. Generally, the resulting change in the direction of the bulge impinging flow enhances down-shelf momentum advection and freshwater delivery into the coastal current. Sensitivity simulations with altered river discharges Q, Coriolis parameter, shelf bottom slope, valley geometry, and ambient stratification show that enhancement of down-shelf freshwater transport in the coastal current, ΔQc, increases with increasing valley depth within the bulge region and decreasing slope Burger number of the ambient shelf. Assuming potential vorticity conservation, a scaling formula of ΔQc/Q is developed, and it agrees well with results of the sensitivity simulations. Mechanisms of valley influences on unforced river plumes revealed here will help future studies of topographic influence on river plumes under more realistic conditions.
    Description: This work is conducted by Canbo Xiao and Weifeng (Gordon) Zhang during CX’s one-year visit at Woods Hole Oceanographic Institution (WHOI) in 2018–19. CX was supported by China Scholarship Council.
    Keywords: Continental shelf/slope ; Buoyancy ; Coastal flows ; Topographic effects ; Runoff ; Numerical analysis/modeling
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  • 95
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Spingys, C. P., Garabato, A. C. N., Legg, S., Polzin, K. L., Abrahamsen, E. P., Buckingham, C. E., Forryan, A., & Frajka-Williams, E. E. Mixing and transformation in a deep western boundary current: a case study. Journal of Physical Oceanography, 51(4), (2021): 1205-1222, https://doi.org/10.1175/JPO-D-20-0132.1
    Description: Water-mass transformation by turbulent mixing is a key part of the deep-ocean overturning, as it drives the upwelling of dense waters formed at high latitudes. Here, we quantify this transformation and its underpinning processes in a small Southern Ocean basin: the Orkney Deep. Observations reveal a focusing of the transport in density space as a deep western boundary current (DWBC) flows through the region, associated with lightening and densification of the current’s denser and lighter layers, respectively. These transformations are driven by vigorous turbulent mixing. Comparing this transformation with measurements of the rate of turbulent kinetic energy dissipation indicates that, within the DWBC, turbulence operates with a high mixing efficiency, characterized by a dissipation ratio of 0.6 to 1 that exceeds the common value of 0.2. This result is corroborated by estimates of the dissipation ratio from microstructure observations. The causes of the transformation are unraveled through a decomposition into contributions dependent on the gradients in density space of the: dianeutral mixing rate, isoneutral area, and stratification. The transformation is found to be primarily driven by strong turbulence acting on an abrupt transition from the weakly stratified bottom boundary layer to well-stratified off-boundary waters. The reduced boundary layer stratification is generated by a downslope Ekman flow associated with the DWBC’s flow along sloping topography, and is further regulated by submesoscale instabilities acting to restratify near-boundary waters. Our results provide observational evidence endorsing the importance of near-boundary mixing processes to deep-ocean overturning, and highlight the role of DWBCs as hot spots of dianeutral upwelling.
    Description: CS, ACNG, AF, and EFW were supported by the U.K. Natural Environment Research Council (NERC) Grant NE/K013181/1. ACNG was supported by the Royal Society and Wolfson Foundation. EPA and CEB were supported by NERC Grant NE/K012843/1. CEB was funded by an MSCA grant (No. 798319) from the European Union’s Horizon 2020 program. EPA was supported by NERC Grant NE/N018095/1. SL and KP were supported by U.S. National Science Foundation Grants OCE-1536453 and OCE-1536779. SL acknowledges support of Award NA18OAR4320123 from the National Oceanic and Atmospheric Administration, U.S. Department of Commerce. The statements, findings, conclusions, and recommendations are those of the authors, and do not necessarily reflect the views of the National Oceanic and Atmospheric Administration, or the U.S. Department of Commerce.
    Keywords: Bottom currents ; Diapycnal mixing ; Turbulence ; Southern Ocean
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  • 96
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Hawkings, J. R., Linhoff, B. S., Wadham, J. L., Stibal, M., Lamborg, C. H., Carling, G. T., Lamarche-Gagnon, G., Kohler, T. J., Ward, R., Hendry, K. R., Falteisek, L., Kellerman, A. M., Cameron, K. A., Hatton, J. E., Tingey, S., Holt, A. D., Vinsova, P., Hofer, S., Bulinova, M., Větrovský, T., Meire, L., Spencer, R. G. M. Large subglacial source of mercury from the southwestern margin of the Greenland Ice Sheet. Nature Geoscience, 14, (2021): 496-502, https://doi.org/10.1038/s41561-021-00753-w.
    Description: The Greenland Ice Sheet is currently not accounted for in Arctic mercury budgets, despite large and increasing annual runoff to the ocean and the socio-economic concerns of high mercury levels in Arctic organisms. Here we present concentrations of mercury in meltwaters from three glacial catchments on the southwestern margin of the Greenland Ice Sheet and evaluate the export of mercury to downstream fjords based on samples collected during summer ablation seasons. We show that concentrations of dissolved mercury are among the highest recorded in natural waters and mercury yields from these glacial catchments (521–3,300 mmol km−2 year−1) are two orders of magnitude higher than from Arctic rivers (4–20 mmol km−2 year−1). Fluxes of dissolved mercury from the southwestern region of Greenland are estimated to be globally significant (15.4–212 kmol year−1), accounting for about 10% of the estimated global riverine flux, and include export of bioaccumulating methylmercury (0.31–1.97 kmol year−1). High dissolved mercury concentrations (~20 pM inorganic mercury and ~2 pM methylmercury) were found to persist across salinity gradients of fjords. Mean particulate mercury concentrations were among the highest recorded in the literature (~51,000 pM), and dissolved mercury concentrations in runoff exceed reported surface snow and ice values. These results suggest a geological source of mercury at the ice sheet bed. The high concentrations of mercury and its large export to the downstream fjords have important implications for Arctic ecosystems, highlighting an urgent need to better understand mercury dynamics in ice sheet runoff under global warming.
    Description: This research is part of a European Commission Horizon 2020 Marie Skłodowska-Curie Actions fellowship ICICLES (grant agreement #793962) to J.R.H. Greenland terrestrial research campaigns were funded by a UK NERC standard grant (NE/I008845/1) and a Leverhulme Trust Research Grant (RPG-2016-439) to J.L.W., with additional support provided by a Royal Society Wolfson Merit Award to J.L.W. Additional funding came from Czech Science Foundation grants (GACR; 15-17346Y and 18-12630S) to M.S. Fjord fieldwork was supported by European Research Council grant ICY-LAB (grant agreement 678371) and Royal Society Enhancement Award (grant RGF\EA\181036) to K.R.H. L.M. was funded by research programme VENI (0.16.Veni.192.150, NWO). T.J.K. was supported by Charles University Research Centre program no. 204069. The authors thank the captain and crew of the RV Kisaq and staff at the Greenland Institute of Natural Resources for assistance during fjord fieldwork, and all those involved with fieldwork at Leverett Camp during the 2012 and 2015 field campaigns. M. Cooper is thanked for providing the geological overview file for Extended Data Fig. 1a, and K. Mankoff for help in generating the modelled GrIS discharge datasets. The authors also thank G. White in the geochemistry group at the National High Magnetic Field Geochemistry Laboratory, which is supported by NSF DMR-1644779 and the State of Florida, for analytical support.
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  • 97
    Publication Date: 2022-05-27
    Description: Author Posting. © American Meteorological Society, 2021. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 51(3), (2021): 955–973, https://doi.org/10.1175/JPO-D-20-0240.1.
    Description: Fresh Arctic waters flowing into the Atlantic are thought to have two primary fates. They may be mixed into the deep ocean as part of the overturning circulation, or flow alongside regions of deep water formation without impacting overturning. Climate models suggest that as increasing amounts of freshwater enter the Atlantic, the overturning circulation will be disrupted, yet we lack an understanding of how much freshwater is mixed into the overturning circulation’s deep limb in the present day. To constrain these freshwater pathways, we build steady-state volume, salt, and heat budgets east of Greenland that are initialized with observations and closed using inverse methods. Freshwater sources are split into oceanic Polar Waters from the Arctic and surface freshwater fluxes, which include net precipitation, runoff, and ice melt, to examine how they imprint the circulation differently. We find that 65 mSv (1 Sv ≡ 106 m3 s−1) of the total 110 mSv of surface freshwater fluxes that enter our domain participate in the overturning circulation, as do 0.6 Sv of the total 1.2 Sv of Polar Waters that flow through Fram Strait. Based on these results, we hypothesize that the overturning circulation is more sensitive to future changes in Arctic freshwater outflow and precipitation, while Greenland runoff and iceberg melt are more likely to stay along the coast of Greenland.
    Description: We gratefully acknowledge the U.S. National Science Foundation: this work was supported by Grants OCE-1258823, OCE-1756272, OCE-1948335, and OCE-2038481. L.H.S. thanks the U.S. Norway Fulbright Foundation for the Norwegian Arctic Chair Grant 2019-20 that made the visit to Scripps Institution of Oceanography possible. N.P.H. acknowledges support by the U.K. Natural Environment Research Council (NERC) National Capability program CLASS (NE/R015953/1), and Grants U.K.-OSNAP (NE/K010875/1, NE/K010875/2) and U.K.-OSNAP Decade (NE/T00858X/1). We acknowledge the World Climate Research Programme, which, through its Working Group on Coupled Modelling, coordinated and promoted CMIP6.
    Keywords: Arctic ; North Atlantic Ocean ; Conservation equations ; Meridional overturning circulation ; Ocean circulation ; Inverse methods
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  • 98
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Li, F., Lozier, M. S., Bacon, S., Bower, A. S., Cunningham, S. A., de Jong, M. F., DeYoung, B., Fraser, N., Fried, N., Han, G., Holliday, N. P., Holte, J., Houpert, L., Inall, M. E., Johns, W. E., Jones, S., Johnson, C., Karstensen, J., Le Bras, I. A., P. Lherminier, X. Lin, H. Mercier, M. Oltmanns, A. Pacini, T. Petit, R. S. Pickart, D. Rayner, F. Straneo, V. Thierry, M. Visbeck, I. Yashayaev & Zhou, C. Subpolar North Atlantic western boundary density anomalies and the Meridional Overturning Circulation. Nature Communications, 12(1), (2021): 3002, https://doi.org/10.1038/s41467-021-23350-2.
    Description: Changes in the Atlantic Meridional Overturning Circulation, which have the potential to drive societally-important climate impacts, have traditionally been linked to the strength of deep water formation in the subpolar North Atlantic. Yet there is neither clear observational evidence nor agreement among models about how changes in deep water formation influence overturning. Here, we use data from a trans-basin mooring array (OSNAP—Overturning in the Subpolar North Atlantic Program) to show that winter convection during 2014–2018 in the interior basin had minimal impact on density changes in the deep western boundary currents in the subpolar basins. Contrary to previous modeling studies, we find no discernable relationship between western boundary changes and subpolar overturning variability over the observational time scales. Our results require a reconsideration of the notion of deep western boundary changes representing overturning characteristics, with implications for constraining the source of overturning variability within and downstream of the subpolar region.
    Description: We acknowledge funding from the Physical Oceanography Program of the U.S. National Science Foundation (OCE-1259398, OCE-1756231, OCE-1948335); the U.K. Natural Environment Research Council (NERC) National Capability programs the Extended Ellett Line and CLASS (NE/R015953/1), and NERC grants UK-OSNAP (NE/K010875/1, NE/K010875/2, NE/K010700/1) and U.K. OSNAP Decade (NE/T00858X/1, NE/T008938/1). Additional support was received from the European Union 7th Framework Program (FP7 2007-2013) under grant 308299 (NACLIM), the Horizon 2020 research and innovation program under grants 727852 (Blue-Action), 862626 (EuroSea). We also acknowledge support from the Royal Netherlands Institute for Sea Research, the Surface Water and Ocean Topography-Canada (SWOT-C), Canadian Space Agency, the Aquatic Climate Change Adaptation Services Program (ACCASP), Fisheries and Oceans Canada, an Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant, and from the China’s national key research and development projects (2016YFA0601803), the National Natural Science Foundation of China (41925025) and the Fundamental Research Funds for the Central Universities (201424001). Support for the 53°N array by the RACE program of the German Ministry BMBF is acknowledged, as is the contribution from Fisheries and Oceans Canada’s Atlantic Zone Monitoring Program.
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  • 99
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Pankov, K., McArthur, A. G., Gold, D. A., Nelson, D. R., Goldstone, J., & Wilson, J. Y. The cytochrome P450 (CYP) superfamily in cnidarians. Scientific Reports, 11(1), (2021): 9834, https://doi.org/10.1038/s41598-021-88700-y.
    Description: The cytochrome P450 (CYP) superfamily is a diverse and important enzyme family, playing a central role in chemical defense and in synthesis and metabolism of major biological signaling molecules. The CYPomes of four cnidarian genomes (Hydra vulgaris, Acropora digitifera, Aurelia aurita, Nematostella vectensis) were annotated; phylogenetic analyses determined the evolutionary relationships amongst the sequences and with existing metazoan CYPs. 155 functional CYPs were identified and 90 fragments. Genes were from 24 new CYP families and several new subfamilies; genes were in 9 of the 12 established metazoan CYP clans. All species had large expansions of clan 2 diversity, with H. vulgaris having reduced diversity for both clan 3 and mitochondrial clan. We identified potential candidates for xenobiotic metabolism and steroidogenesis. That each genome contained multiple, novel CYP families may reflect the large evolutionary distance within the cnidarians, unique physiology in the cnidarian classes, and/or different ecology of the individual species.
    Description: This study was supported by grants from the Natural Science Engineering Research Council of Canada in the form of a Discovery Grant (RGPIN-328204-2011 and RGPIN-05767-2016) to J.Y.W. A.G.M. held a Cisco Research Chair in Bioinformatics, supported by Cisco Systems Canada, Inc. K.V.P. was supported by a Natural Sciences and Engineering Research Council of Canada (NSERC) Undergraduate Student Research Award (USRA). Computer resources were supplied by the McMaster Service Lab and Repository computing cluster, funded in part by grants to A.G.M. from the Canadian Foundation for Innovation (34531 to A.G.M.).
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  • 100
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Ashjian, C. J., Okkonen, S. R., Campbell, R. G., & Alatalo, P. Lingering Chukchi Sea sea ice and Chukchi Sea mean winds influence population age structure of euphausiids (krill) found in the bowhead whale feeding hotspot near Pt. Barrow, Alaska. Plos One, 16(7), (2021): e0254418, https://doi.org/10.1371/journal.pone.0254418.
    Description: Interannual variability in euphausiid (krill) abundance and population structure and associations of those measures with environmental drivers were investigated in an 11-year study conducted in late August–early September 2005–2015 in offshelf waters (bottom depth 〉 40 m) in Barrow Canyon and the Beaufort Sea just downstream of Distributed Biological Observatory site 5 (DBO5) near Pt. Barrow, Alaska. Statistically-significant positive correlations were observed among krill population structure (proportion of juveniles and adults), the volume of Late Season Melt Water (LMW), and late-spring Chukchi Sea sea ice extent. High proportions of juvenile and adult krill were seen in years with larger volumes of LMW and greater spring sea ice extents (2006, 2009, 2012–2014) while the converse, high proportions of furcilia, were seen in years with smaller volumes of LMW and lower spring sea ice extent (2005, 2007, 2010, 2011, 2015). These different life stage, sea ice and water mass regimes represent integrated advective responses to mean fall and/or spring Chukchi Sea winds, driven by prevailing atmospheric pressure distributions in the two sets of years. In years with high proportions of juveniles and adults, late-spring and preceding-fall winds were weak and variable while in years with high proportions of furcilia, late-spring and preceding-fall winds were strong, easterly and consistent. The interaction of krill life history with yearly differences in the northward transports of krill and water masses along with sea ice retreat determines the population structure of late-summer krill populations in the DBO5 region near Pt. Barrow. Years with higher proportions of mature krill may provide larger prey to the Pt. Barrow area bowhead whale prey hotspot. The characteristics of prey near Pt. Barrow is dependent on krill abundance and size, large-scale environmental forcing, and interannual variability in recruitment success of krill in the Bering Sea.
    Description: This research was supported by the National Science Foundation through grants PLR-1023331 (CJA), OPP-0436131 (CJA), PLR-1022139 (RGC), OPP-0436110 (RGC), PLR-1023446 (SRO), and OPP-043166 (SRO), the National Oceanic and Atmospheric Administration (NOAA) under cooperative agreement NA08OAR4320751 with the University of Alaska (SRO) and cooperative agreements NA17RJ1223 and NA09OAR4320129 with the Woods Hole Oceanographic Institution (CJA), the Bureau of Ocean Energy Management through Interagency Agreement 0106RU39923/M08PG20021 between the National Marine Fisheries Service and MMS/BOEM (CJA, RGC, SRO) and through the National Oceanographic Partnership Program award number N00014-08-1-0311 from the Office of Naval Research to the Woods Hole Oceanographic Institution (CJA, SRO, RGC). Additional support was provided by the Coastal Marine Institute at the University of Alaska (SRO, RGC) and the James M. and Ruth P. Clark Arctic Research Initiative Fund at the Woods Hole Oceanographic Institution (CJA). The participation of the K-12 teachers was supported by the National Science Foundation through the ARMADA program at the University of Rhode Island (2005, 2006) and through the POLARTrec program at the Arctic Research Consortium of the United States (2012).
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