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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Molecular and cellular biochemistry 212 (2000), S. 73-79 
    ISSN: 1573-4919
    Keywords: adrenergic receptors ; renin-angiotensin system (RAS) ; gene expression ; kidney
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract To investigate the molecular mechanism(s) of action of catecholamines on the expression of the angiotensinogen (ANG) gene in kidney proximal tubular cells, we used opossum kidney (OK) cells with a fusion gene containing the 5′-flanking regulatory sequence of the rat ANG gene fused with a human growth hormone (hGH) gene as a reporter, pOGH (rANG N-1498/+18), permanently integrated into their genomes. The level of expression of the ANG-GH fusion gene was quantified by the amount of immunoreactive-hGH (IR-hGH) secreted into the medium. The addition of norepinephrine (NE), isoproterenol (a β1/β2-adrenergic receptor (AR) agonist) and iodoclonidine (an α2-AR agonist) stimulated the expression of the ANG-GH fusion gene in a dose-dependent manner, whereas the addition of epinephrine and phenylephrine (α1-AR agonist) had no effect. The stimulatory effect of NE was blocked by the presence of propranolol (β-AR blocker), atenolol (β1-AR blocker), yohimbine (α2-AR blocker), Rp-cAMP (an inhibitor of cAMP-dependent protein kinase AI & AII) and staurosporine (an inhibitor of protein kinase C), but was not blocked by ICI 118, 551 (β2-AR blocker) and prazosin (α1-AR blocker). The addition of a combination of isoproterenol and iodoclonidine or a combination of 8-Bromo-cAMP (8-Br-cAMP) and phorbol 12-myristate (PMA) synergistically stimulated the expression of the ANG-GH fusion gene as compared to the addition of isoproterenol, iodoclonidine, 8-Br-cAMP or PMA alone. Furthermore, the addition of NE, 8-Br-cAMP or PMA stimulated the expression of pOGH (rANG N-806/-779/-53/+18), a fusion gene containing the putative cAMP responsive element (CRE, ANG N-806/-779) upstream of the ANG promoter (ANG N-53/+18) in OK cells, but had no effect on the expression of fusion genes containing the mutant of the CRE. Gel mobility shift assays revealed that the ANG-CRE binds with the DNA-binding domain (bZIP 254-327) of the cAMP-responsive binding protein (CREB). The binding of the labeled ANG-CRE to CREB (bZIP254-327) was displaced by unlabeled ANG-CRE and the CRE of the somatostatin gene but not by the mutants of the ANG-CRE. Finally, NE stimulated the phosphorylation of CREB in OK cells. These studies demonstrate that the molecular mechanism(s) of NE action on the expression of the ANG gene in OK cells may be mediated via both the PKA and PKC signalling pathways and via the phosphorylation of CREB. The phosphorylated CREB then interacts with the CRE in the 5′-flanking region of the ANG gene and subsequently stimulates the gene expression.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 375-376 (Mar. 2008), p. 231-235 
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper introduced the machinability and the state-of-art of Ti6Al4V machining.Turning test with thee different tungsten carbide tool have be done at cutting speed 40, 80, 120, 160m/min, the relationship between cutting force and cutting speed, cutting distance was analyzed, theinfluence of working cutting edge angle on the tool wear and cutting force was investigated as well.Finally, the tool wear and wear mechanics was analyzed
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Acta mechanica Sinica 8 (1992), S. 24-28 
    ISSN: 1614-3116
    Keywords: non-linear wave ; transient wave ; FFT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The transient surface wave motion is studied by a method which transfers the computational region into a unit circle. In place of the Green's formula of harmonic function the Taylor's series of an analytical function is used. The coefficients of the series are determined by FFT. The numerical filtration is accomplished in the Fourier domain.
    Type of Medium: Electronic Resource
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  • 4
    Publication Date: 2020-12-01
    Print ISSN: 0269-7491
    Electronic ISSN: 1873-6424
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by Elsevier
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  • 5
    Publication Date: 2017-04-15
    Description: Backgroud: A previous study has confirmed that the central melanocortin system was able to mediate skeletal muscle AMP-activated protein kinase (AMPK) activation in mice fed a high-fat diet, while activation of the AMPK signaling pathway significantly induced mitochondrial biogenesis. Our hypothesis was that melanocortin 4 receptor (MC4R) was involved in the development of skeletal muscle injury in diabetic rats. Methods: In this study, we treated diabetic rats intracerebroventricularly with MC4R agonist R027-3225 or antagonist SHU9119, respectively. Then, we measured the production of reactive oxygen species (ROS), the levels of malondialdehyde (MDA) and glutathione (GSH), the mitochondrial DNA (mtDNA) content and mitochondrial biogenesis, and the protein levels of p-AMPK, AMPK, peroxisome proliferator-activated receptor-gamma coactivator 1α (PGC-1α), sirtuin 1 (SIRT1) and manganese superoxide dismutase (MnSOD) in the skeletal muscle of diabetic rats. Results: The results showed that there was significant skeletal muscle injury in the diabetic rats along with serious oxidative stress and decreased mitochondrial biogenesis. Treatment with R027-3225 reduced oxidative stress and induced mitochondrial biogenesis in skeletal muscle, and also activated the AMPK-SIRT1-PGC-1α signaling pathway. However, diabetic rats injected with MC4R antagonist SHU9119 showed an aggravated oxidative stress and mitochondrial dysfunction in skeletal muscle. Conclusion: In conclusion, our results revealed that MC4R activation was able to attenuate oxidative stress and mitochondrial dysfunction in skeletal muscle induced by diabetes partially through activating the AMPK-SIRT1-PGC-1α signaling pathway. This article is protected by copyright. All rights reserved
    Electronic ISSN: 0091-7419
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Published by Wiley
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  • 6
    Publication Date: 2016-03-24
    Electronic ISSN: 1932-6203
    Topics: Medicine , Natural Sciences in General
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  • 7
    Publication Date: 2020-01-20
    Description: ©2019. The Authors. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
    Description: A coordinated set of large ensemble atmosphere‐only simulations is used to investigatethe impacts of observed Arctic sea ice‐driven variability (SIDV) on the atmospheric circulation during1979–2014. The experimental protocol permits separating Arctic SIDV from internal variability andvariability driven by other forcings including sea surface temperature and greenhouse gases. The geographicpattern of SIDV is consistent across seven participating models, but its magnitude strongly depends onensemble size. Based on 130 members, winter SIDV is ~0.18 hPa2for Arctic‐averaged sea level pressure(~1.5% of the total variance), and ~0.35 K2for surface air temperature (~21%) at interannual and longertimescales. The results suggest that more than 100 (40) members are needed to separate Arctic SIDV fromother components for dynamical (thermodynamical) variables, and insufficient ensemble size always leadsto overestimation of SIDV. Nevertheless, SIDV is 0.75–1.5 times as large as the variability driven by otherforcings over northern Eurasia and Arctic.
    Description: Published
    Description: e2019GL085397
    Description: 5A. Ricerche polari e paleoclima
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 8
    Publication Date: 2022-03-01
    Description: To examine the atmospheric responses to Arctic sea ice variability in the Northern Hemisphere cold season (from October to the following March), this study uses a coordinated set of large-ensemble experiments of nine atmospheric general circulation models (AGCMs) forced with observed daily varying sea ice, sea surface temperature, and radiative forcings prescribed during the 1979–2014 period, together with a parallel set of experiments where Arctic sea ice is substituted by its climatology. The simulations of the former set reproduce the near-surface temperature trends in reanalysis data, with similar amplitude, and their multimodel ensemble mean (MMEM) shows decreasing sea level pressure over much of the polar cap and Eurasia in boreal autumn. The MMEM difference between the two experiments allows isolating the effects of Arctic sea ice loss, which explain a large portion of the Arctic warming trends in the lower troposphere and drive a small but statistically significant weakening of the wintertime Arctic Oscillation. The observed interannual covariability between sea ice extent in the Barents–Kara Seas and lagged atmospheric circulation is distinguished from the effects of confounding factors based on multiple regression, and quantitatively compared to the covariability in MMEMs. The interannual sea ice decline followed by a negative North Atlantic Oscillation–like anomaly found in observations is also seen in the MMEM differences, with consistent spatial structure but much smaller amplitude. This result suggests that the sea ice impacts on trends and interannual atmospheric variability simulated by AGCMs could be underestimated, but caution is needed because internal atmospheric variability may have affected the observed relationship.
    Description: Published
    Description: 8419–8443
    Description: 2A. Fisica dell'alta atmosfera
    Description: JCR Journal
    Keywords: Arctic ; Sea ice ; Atmospheric circulation ; Climate models ; 01.01. Atmosphere
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 9
    Publication Date: 2023-03-08
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Suo, L., Gastineau, G., Gao, Y., Liang, Y.-C., Ghosh, R., Tian, T., Zhang, Y., Kwon, Y.-O., Otterå, O., Yang, S., & Matei, D. Simulated contribution of the interdecadal Pacific oscillation to the west Eurasia cooling in 1998–2013. Environmental Research Letters, 17(9), (2022): 094021, https://doi.org/10.1088/1748-9326/ac88e5.
    Description: Large ensemble simulations with six atmospheric general circulation models involved are utilized to verify the interdecadal Pacific oscillation (IPO) impacts on the trend of Eurasian winter surface air temperatures (SAT) during 1998–2013, a period characterized by the prominent Eurasia cooling (EC). In our simulations, IPO brings a cooling trend over west-central Eurasia in 1998–2013, about a quarter of the observed EC in that area. The cooling is associated with the phase transition of the IPO to a strong negative. However, the standard deviation of the area-averaged SAT trends in the west EC region among ensembles, driven by internal variability intrinsic due to the atmosphere and land, is more than three times the isolated IPO impacts, which can shadow the modulation of the IPO on the west Eurasia winter climate.
    Description: This work is funded by the JPI Climate-Oceans ROADMAP and the Blue‐Action Project (European Union's Horizon 2020 research and innovation program, 727852). The CAM6-Nor simulations were performed on resources provided by UNINETT Sigma2—the National Infrastructure for High Performance Computing and Data Storage in Norway (nn2343k, NS9015K). The simulations of IAP4.1 were supported by National Key R&D Program of China 2017YFE0111800.
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 10
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Liang, Y., Kwon, Y., Frankignoul, C., Danabasoglu, G., Yeager, S., Cherchi, A., Gao, Y., Gastineau, G., Ghosh, R., Matei, D., Mecking, J., V., Peano, D., Suo, L., & Tian, T. Quantification of the arctic sea ice-driven atmospheric circulation variability in coordinated large ensemble simulations. Geophysical Research Letters, 47(1), (2020): e2019GL085397, doi:10.1029/2019GL085397.
    Description: A coordinated set of large ensemble atmosphere‐only simulations is used to investigate the impacts of observed Arctic sea ice‐driven variability (SIDV) on the atmospheric circulation during 1979–2014. The experimental protocol permits separating Arctic SIDV from internal variability and variability driven by other forcings including sea surface temperature and greenhouse gases. The geographic pattern of SIDV is consistent across seven participating models, but its magnitude strongly depends on ensemble size. Based on 130 members, winter SIDV is ~0.18 hPa2 for Arctic‐averaged sea level pressure (~1.5% of the total variance), and ~0.35 K2 for surface air temperature (~21%) at interannual and longer timescales. The results suggest that more than 100 (40) members are needed to separate Arctic SIDV from other components for dynamical (thermodynamical) variables, and insufficient ensemble size always leads to overestimation of SIDV. Nevertheless, SIDV is 0.75–1.5 times as large as the variability driven by other forcings over northern Eurasia and Arctic.
    Description: The authors thank Editor Christina Patricola and two anonymous reviewers for their comprehensive and insightful comments, which have led to improved presentation of this manuscript. We acknowledge support by the Blue‐Action Project (European Union's Horizon 2020 research and innovation program, 727852, http://www.blue‐action.eu/index.php?id = 3498). The WHOI‐NCAR group is also supported by the US National Science Foundation (NSF) Office of Polar Programs Grants 1736738 and 1737377, and their computing and data storage resources, including the Cheyenne supercomputer (doi:10.5065/D6RX99HX), were provided by the Computational and Information Systems Laboratory at NCAR. NCAR is a major facility sponsored by the U.S. NSF under Cooperative Agreement 1852977. The LOCEAN‐IPSL group was granted access to the HPC resources of TGCC under the Allocation A5‐017403 made by GENCI. The SST and SIC data were downloaded from the U.K. Met Office Hadley Centre Observations Datasets (http://www.metoffice.gov.uk/hadobs/hadisst).
    Repository Name: Woods Hole Open Access Server
    Type: Article
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