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  • Nature Research  (112)
  • Wiley  (102)
  • American Association for the Advancement of Science (AAAS)
  • Cell Press
  • Irkutsk : Ross. Akad. Nauk, Sibirskoe Otd., Inst. Zemnoj Kory
  • Krefeld : Geologischer Dienst Nordhein-Westfalen
  • 2020-2023  (221)
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  • 1
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    Wiley
    In:  EPIC3Journal of Geophysical Research-Atmospheres, Wiley, 127(3), pp. 1-18, ISSN: 0148-0227
    Publication Date: 2022-02-28
    Description: Fram Strait in the northern North Atlantic is a key region for marine cold air outbreaks (MCAOs), southward discharges of polar air under northerly air flow, which have a strong impact on air-sea heat fluxes, boundary layer processes and severe weather. This study investigates climatologies and decadal trends of Fram Strait MCAOs of different intensity classes based on the ERA5 reanalysis product for 1979–2020. Among striking interannual variability, it is shown that the main MCAO season is December through March, when MCAOs occur around 2/3 of the time. We report on significant decadal MCAO decreases in December and January, and a significant increase in March. While the mid-winter decrease is mainly related to the different paces of warming between the surface and the lower atmosphere, the increase in March can be related to changes in synoptic circulation patterns. As an explanation for the latter, a possible feedback between retreating Barents Sea sea ice, enhanced cyclonic activity and Fram Strait MCAOs is postulated. Exemplifying the trend toward stronger MCAOs during March, the study details the recordbreaking MCAO season in early 2020, and an observational case study of an extreme MCAO event in March 2020 is conducted. Thereby, radiosonde observations are combined with kinematic air back-trajectories to provide rare observational evidence for the diabatic cooling and drying during the MCAO preconditioning phase.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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  • 2
    Publication Date: 2022-04-24
    Description: Hillaire‐Marcelet al. bring forward several physical and geochemical arguments against our finding of an Arctic glaciolacustrine system in the past. In brief, we find that a physical approach to further test our hypothesis should additionally consider the actual bathymetry of the Greenland–Scotland Ridge (GSR), the density maximum of freshwater at 3–4°C, the sensible heat flux from rivers, and the actual volumes that are being mixed and advected. Their geochemical considerations acknowledge our original argument, but they also add a number of assumptions that are neither required to explain the observations, nor do they correspond to the lithology of the sediments. Rather than being additive in nature, their arguments of high particle flux, low particle flux, export of 230Th and accumulation of 230Th, are mutually exclusive. We first address the arguments above, before commenting on some misunderstandings of our original claim in their contribution, especially regarding our dating approach.
    Repository Name: EPIC Alfred Wegener Institut
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  • 3
    Publication Date: 2022-05-03
    Description: Shallow seabed depressions attributed to focused fluid seepage, known as pock- marks, have been documented in all continental margins. In this study, we dem- onstrate how pockmark formation can be the result of a combination of multiple factors— fluid type, overpressures, seafloor sediment type, stratigraphy and bot- tom currents. We integrate multibeam echosounder and seismic reflection data, sediment cores and pore water samples, with numerical models of groundwa- ter and gas hydrates, from the Canterbury Margin (off New Zealand). More than 6800 surface pockmarks, reaching densities of 100 per km2, and an undefined number of buried pockmarks, are identified in the middle to outer shelf and lower continental slope. Fluid conduits across the shelf and slope include shal- low to deep chimneys/pipes. Methane with a biogenic and/or thermogenic origin is the main fluid forming flow and escape features, although saline and fresh- ened groundwaters may also be seeping across the slope. The main drivers of fluid flow and seepage are overpressure across the slope generated by sediment loading and thin sediment overburden above the overpressured interval in the outer shelf. Other processes (e.g. methane generation and flow, a reduction in hydrostatic pressure due to sea- level lowering) may also account for fluid flow and seepage features, particularly across the shelf. Pockmark occurrence coin- cides with muddy sediments at the seafloor, whereas their planform is elongated by bottom currents.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , peerRev
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  • 4
    Publication Date: 2022-03-16
    Description: Tectono-stratigraphic interpretation and sequential restoration modelling was performed over two high-resolution seismic profiles crossing the Western Ionian Basin of southern Italy. This analysis was undertaken in order to provide greater insights and a more reliable assessment of the deformation rate affecting the area. Offshore seismic profiling illuminates the sub-seafloor setting where a belt of active normal faults slice across the foot of the Malta Escarpment, a regional-scale structural boundary inherited from the Permo-Triassic palaeotectonic setting. A sequential restoration workflow was established to back-deform the entire investigated sector with the primary aim of analysing the deformation history of the three major normal faults affecting the area. Restoration of the tectono-stratigraphic model reveals how deformation rates evolved through time. In the early stage, the studied area experienced a significant deformation with the horizontal component prevailing over the vertical element. In this context, the three major faults contribute to only one third of the total deformation. The overall throw and extension then notably reduced through time towards the present day and, since the middle Pliocene, ongoing crustal deformation is accommodated almost entirely by the three major normal faults. Unloading and decompaction indicate that when compared to the unrestored seismic sections, a revision and a reduction of roughly one third of the vertical displacement of the faults offset is required. This analysis ultimately allows us to better understand the seismic potential of the region.
    Description: Published
    Description: 321-341
    Description: 2T. Deformazione crostale attiva
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 5
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    Wiley
    In:  EPIC3Limnology and Oceanography Letters, Wiley, 7(2), pp. 167-174, ISSN: 2378-2242
    Publication Date: 2022-03-25
    Description: The end of the polar night with the concurrent onset of photosynthetic biomass production ultimately leads to the spring bloom, which represents the most important event of primary production for the Arctic marine ecosystem. This dataset shows, for the first time, significant in situ biomass accumulation during the dark–light transition in the high Arctic, as well as the earliest recorded positive net primary production rates together with constant chlorophyll a-normalized potential for primary production through winter and spring. The results indicate a high physiological capacity to perform photosynthesis upon re-illumination, which is in the same range as that observed during the spring bloom. Put in context with other data, the results of this study indicate that also active cells originating from the low winter standing stock in the water column, rather than solely resting stages from the sediment, can seed early spring bloom assemblages.
    Repository Name: EPIC Alfred Wegener Institut
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  • 6
    Publication Date: 2022-02-17
    Description: Free access at https://onlinelibrary.wiley.com/doi/10.1111/1755-6724.14824
    Description: Earthquake is a sudden release of energy due to fault motions. The severity of the damages can be minimized by development of a culture of prevention which includes the Seismic Hazard Assessment, microzonation studies and appropriate building codes. Earthquake risk assessment methods require seismo tectonic information usually organized in earthquake catalogues utilized in Probabilistic Seismic Hazard Assessment (PSHA) based on initial work by Cornell (1968), where probability distributions for magnitudes and source site distances reported in earthquake catalogues were utilized for the first time. In following years the method furtherly improved reporting an upper bound on the earthquake magnitude in each region avoiding the inclusion of unrealistically big earthquakes. A different approach has been followed in Countries characterized by significant incompletenesses in available earthquake catalogues. In these places the Deterministic Seismic Hazard Assessment (DSHA) methods have been often utilized. In particular the DSHA takes into account the maximum possible earthquake to evaluate the intensity of seismic ground motion distribution at a site by taking account the seismotectonic setup of the area. A deepening in the knowledge of seismotectonics and of morphostructural features of the studied area has been carried out in pattern recognition studies (Gelfand et al., 1976 and references therein). More updated applications named Neo-Deterministic Seismic Hazard Assessment (NDSHA) proposed by Wang et al. (2021) also consider morphostructural zoning which, in turn, considers nodes (fractured areas), lineaments and topographical features like the maximal elevation and the minimal elevation of the studied area. The steepness of topographic surfaces and sharp variations in morphostructural parameters indicate high tectonic activity. Some geological features are also presently utilized in PSHA methods in some Countries and considers basic parameters like the top and the bottom of seismogenic layers deduced by faults geometry within the frame of the Earthquake Rupture Forecasting (Bird and Liu, 2007).
    Description: Published
    Description: 31-33
    Description: 9T. Geochimica dei fluidi applicata allo studio e al monitoraggio di aree sismiche
    Description: JCR Journal
    Keywords: probabilistic seismic hazard assessment, deterministic seismic hazard assessment, helium isotopes, geochemical prospection, earthquake precursors ; seismic hazard estimation by geochemical methods
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 7
    Publication Date: 2022-02-21
    Description: Relative sea‐level (RSL) evolution during Marine Isotopic Stage (MIS) 5 in the Mediterranean basin is still not fully understood despite a plethora of morphological, stratigraphic and geochronological studies carried out on highstand deposits of this area. In this review we assembled a database of 323 U/Th‐dated samples (e.g. corals, molluscs, speleothems) which were used to chronologically constrain RSL evolution within MIS 5. The application of strict geochemical criteria to the U/Th samples indicates that only ~33% of data available for the Mediterranean Sea can be considered ‘reliable’. Most of these data (~65%) refer to the MIS 5e highstand, while only ~17% could be related to the MIS 5a. No attribution to MIS 5c can be unequivocally supported. Nevertheless, the resulting framework does not allow us to define a satisfactory RSL trend during the MIS 5e highstand and subsequent MIS 5 substages. Overall, the proposed selection of reliable/unreliable data would be useful for detecting areas where MIS 5 substage attributions are not supported by confident U/Th chronological data and thus the related reconstructions need to be revised. In this regard, the resulting framework calls for a reappraisal and re‐examination of the Mediterranean records with advanced geochronological methodologies.
    Description: Published
    Description: 1174-1189
    Description: 5A. Ricerche polari e paleoclima
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 8
    Publication Date: 2022-02-25
    Description: The Apennines are a retreating collisional belt where the foreland basin system, across large domains, is floored by a subaerial forebulge unconformity developed due to forebulge uplift and erosion. This unconformity is overlain by a diachronous sequence of three lithostratigraphic units made of (a) shallow-water carbonates, (b) hemipelagic marls and shales and (c) siliciclastic turbidites. Typically, the latter two have been interpreted regionally as the onset of syn-orogenic deposition in the foredeep depozone, whereas little attention has been given to the underlying unit. Accordingly, the rate of migration of the central-southern Apennine fold-thrust beltforeland basin system has been constrained, so far, exclusively considering the age of the hemipelagites and turbidites, which largely post-date the onset of foredeep depozone. In this work, we provide new high-resolution ages obtained by strontium isotope stratigraphy applied to calcitic bivalve shells sampled at the base of the first syn-orogenic deposits overlying the Eocene-Cretaceous pre-orogenic substratum. Integration of our results with published data indicates progressive rejuvenation of the strata sealing the forebulge unconformity towards the outer portions of the foldthrust belt. In particular, the age of the forebulge unconformity linearly scales with the pre-orogenic position of the analysed sites, pointing to an overall constant migration velocity of the forebulge wave in the last 25 Myr.
    Description: Published
    Description: 2817-2836
    Description: 6A. Geochimica per l'ambiente e geologia medica
    Description: JCR Journal
    Keywords: central-southern Apennines (Italy) ; fold-thrust belt ; forebulge ; foredeep
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 9
    Publication Date: 2022-01-11
    Description: Mt Etna has made headlines over the last weeks and months with spectacular eruptions, some of them highly explosive. This type of paroxysmal eruptive behaviour is characteristic of Etna’s activity over the past few decades and so it is no surprise that Etna is among the most active volcanoes worldwide. Etna is well-known for its extraordinary geology and due to its repeated eruptive activity it provides a continuous supply of new scientific opportunities to understand the inner workings of large basaltic volcanic systems. In addition to its scientific value, Etna is also a world famous tourist attraction and has been listed as a UNESCO World Heritage site in 2013 for its geological and cultural value and not least for its fine agricultural products. Etna’s status as an iconic volcano is not a recent phenomenon; in fact, Etna has been a literary fixture for at least 3000 years, giving rise to many ancient myths and legends that mark it as a special place, deserving of human respect. From the ancient eruptions to the latest events in February–April 2021, people try to explain and understand the processes that occur within and beneath the volcano. In this article, we briefly summarize the recent eruptive activity of Etna as well as the ancient myths and legends that surround this volcano, from the underground forge of Hephaestus to the adventures of Odysseus, all the way to the benefits and dangers the volcano provides to those living on its flanks today.
    Description: Published
    Description: 141-149
    Description: 2TM. Divulgazione Scientifica
    Description: N/A or not JCR
    Keywords: Etna, mythology, 2021 paroxysms, economy ; 04.08. Volcanology
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 10
    Publication Date: 2022-11-07
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Kourantidou, M., & Jin, D. Mesopelagic-epipelagic fish nexus in viability and feasibility of commercial-scale mesopelagic fisheries. Natural Resource Modeling, 35(4), (2022): e12350, https://doi.org/10.1111/nrm.12350.
    Description: While considerable scientific uncertainties persist for mesopelagic ecosystems, the fishing industry has developed a great interest in commercial exploitation with improved technologies as part of their search for new sources of feed for fishmeal and fish oil for aquaculture, which will intensify with the planet's growing population. The multiple uncertainties surrounding the ecosystem structure and particularly the size of biomass, hinder a good understanding of the risks associated with large-scale exploitation, which is needed for a management framework for sustainable ocean uses. Despite concerns regarding irreversible losses triggered by commercial fishing, work exploring the vulnerability of mesopelagic fish to harvesting is largely missing. This study investigates the economic feasibility of mesopelagic fishing which is the primary driver for any possible future expansion. Using very limited information currently available, we conduct a high-level assessment focusing on key ecological and economic interactions and develop an initial understanding of the economic feasibility of commercial harvesting for mesopelagic fish in the coming years. We conduct simulations using a classical bioeconomic model that captures two species groups, mesopelagic and epipelagic fish, using a wide range of price and cost parameters. We analyze different scenarios for the economic profitability of the fishery in a regional fishery management context. The results of our study highlight the importance of better understanding key biological and ecological mechanisms and parameters which can in turn help inform policies aimed at protecting the mesopelagic.
    Description: This study is supported by WHOI's Ocean Twilight Zone program which is part of the Audacious Project, a collaborative endeavor, housed at TED.
    Keywords: Bioeconomic analysis ; Commercial fisheries ; Ecological interactions ; Economic feasibility ; Mesopelagic fish ; Twilight zone
    Repository Name: Woods Hole Open Access Server
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  • 11
    Publication Date: 2022-11-15
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Shero, M. R., Kirkham, A. L., Costa, D. P., & Burns, J. M. Iron mobilization during lactation reduces oxygen stores in a diving mammal. Nature Communications, 13(1), (2022): 4322, https://doi.org/10.1038/s41467-022-31863-7.
    Description: The profound impacts that maternal provisioning of finite energy resources has on offspring survival have been extensively studied across mammals. This study shows that in addition to calories, high hemoprotein concentrations in diving mammals necessitates exceptional female-to-pup iron transfer. Numerous indices of iron mobilization (ferritin, serum iron, total-iron-binding-capacity, transferrin saturation) were significantly elevated during lactation in adult female Weddell seals (Leptonychotes weddellii), but not in skip-breeders. Iron was mobilized from endogenous stores for incorporation into the Weddell seal’s milk at concentrations up to 100× higher than terrestrial mammals. Such high rates of iron offload to offspring drew from the female’s own heme stores and led to compromised physiologic dive capacities (hemoglobin, myoglobin, and total body oxygen stores) after weaning their pups, which was further reflected in shorter dive durations. We demonstrate that lactational iron transfer shapes physiologic dive thresholds, identifying a cost of reproduction to a marine mammal.
    Description: This research was conducted with support from NSF ANT-0838892 to DPC; ANT-0838937 and ANT-1246463 to JMB (which also supported ALK and MRS); and The Investment in Science Fund at WHOI to MRS.
    Keywords: Animal physiology ; Ecophysiology ; Homeostasis
    Repository Name: Woods Hole Open Access Server
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  • 12
    Publication Date: 2022-11-18
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Shu, Y., Nielsen, S. G., Le Roux, V., Wörner, G., Blusztajn, J., & Auro, M. Sources of dehydration fluids underneath the Kamchatka arc. Nature Communications, 13(1), (2022): 4467, https://doi.org/10.1038/s41467-022-32211-5.
    Description: Fluids mediate the transport of subducted slab material and play a crucial role in the generation of arc magmas. However, the source of subduction-derived fluids remains debated. The Kamchatka arc is an ideal subduction zone to identify the source of fluids because the arc magmas are comparably mafic, their source appears to be essentially free of subducted sediment-derived components, and subducted Hawaii-Emperor Seamount Chain (HESC) is thought to contribute a substantial fluid flux to the Kamchatka magmas. Here we show that Tl isotope ratios are unique tracers of HESC contribution to Kamchatka arc magma sources. In conjunction with trace element ratios and literature data, we trace the progressive dehydration and melting of subducted HESC across the Kamchatka arc. In succession, serpentine (〈100 km depth), lawsonite (100–250 km depth) and phengite (〉250 km depth) break down and produce fluids that contribute to arc magmatism at the Eastern Volcanic Front (EVF), Central Kamchatka Depression (CKD), and Sredinny Ridge (SR), respectively. However, given the Tl-poor nature of serpentine and lawsonite fluids, simultaneous melting of subducted HESC is required to explain the HESC-like Tl isotope signatures observed in EVF and CKD lavas. In the absence of eclogitic crust melting processes in this region of the Kamchatka arc, we propose that progressive dehydration and melting of a HESC-dominated mélange offers the most compelling interpretation of the combined isotope and trace element data.
    Description: This study was financially supported by grants from the National Natural Science Foundation of China (NSFC) (Grant No. 41903008) and Chinese Postdoctoral Science Foundation (Grant No. 2019M660153) to Y.S., NSF (Grant No. EAR-1829546) to S.G.N. and NSF (Grant No. EAR-1855302) to V.L.R.
    Keywords: Geochemistry ; Marine chemistry
    Repository Name: Woods Hole Open Access Server
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  • 13
    Publication Date: 2022-11-18
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Tian, Y., Liu, X., Li, J., Deng, Y., DeGiorgis, J. A., Zhou, S., Caratenuto, A., Minus, M. L., Wan, Y., Xiao, G., & Zheng, Y. Farm-waste-derived recyclable photothermal evaporator. Cell Reports Physical Science, 2(9), (2021): 100549, https://doi.org/10.1016./j.xcrp.2021.100549
    Description: Interfacial solar steam generation is emerging as a promising technique for efficient desalination. Although increasing efforts have been made, challenges exist for achieving a balance among a plethora of performance indicators—for example, rapid evaporation, durability, low-cost deployment, and salt rejection. Here, we demonstrate that carbonized manure can convert 98% of sunlight into heat, and the strong capillarity of porous carbon fibers networks pumps sufficient water to evaporation interfaces. Salt diffusion within microchannels enables quick salt drainage to the bulk seawater to prevent salt accumulation. With these advantages, this biomass-derived evaporator is demonstrated to feature a high evaporation rate of 2.81 kg m−2 h−1 under 1 sun with broad robustness to acidity and alkalinity. These advantages, together with facial deployment, offer an approach for converting farm waste to energy with high efficiency and easy implementation, which is particularly well suited for developing regions.
    Description: This project is supported by the National Science Foundation through grant no. CBET-1941743. This project is based upon work supported in part by the National Science Foundation under EPSCoR Cooperative Agreement no. OIA-1655221.
    Keywords: Biomass ; Recyclable ; Manure ; Farm waste ; Photothermal evaporation ; Desalination
    Repository Name: Woods Hole Open Access Server
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  • 14
    Publication Date: 2022-06-27
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Suca, J., Ji, R., Baumann, H., Pham, K., Silva, T., Wiley, D., Feng, Z., & Llopiz, J. Larval transport pathways from three prominent sand lance habitats in the Gulf of Maine. Fisheries Oceanography, 31(3), (2022): 333– 352, https://doi.org/10.1111/fog.12580.
    Description: Northern sand lance (Ammodytes dubius) are among the most critically important forage fish throughout the Northeast US shelf. Despite their ecological importance, little is known about the larval transport of this species. Here, we use otolith microstructure analysis to estimate hatch and settlement dates of sand lance and then use these measurements to parametrize particle tracking experiments to assess the source–sink dynamics of three prominent sand lance habitats in the Gulf of Maine: Stellwagen Bank, the Great South Channel, and Georges Bank. Our results indicate the pelagic larval duration of northern sand lance lasts about 2 months (range: 50–84 days) and exhibit a broad range of hatch and settlement dates. Forward and backward particle tracking experiments show substantial interannual variability, yet suggest transport generally follows the north to south circulation in the Gulf of Maine region. We find that Stellwagen Bank is a major source of larvae for the Great South Channel, while the Great South Channel primarily serves as a sink for larvae from Stellwagen Bank and Georges Bank. Retention is likely the primary source of larvae on Georges Bank. Retention within both Georges Bank and Stellwagen Bank varies interannually in response to changes in local wind events, while the Great South Channel only exhibited notable retention in a single year. Collectively, these results provide a framework to assess population connectivity among these sand lance habitats, which informs the species' recruitment dynamics and impacts its vulnerability to exploitation.
    Description: Funding came from the National Oceanic and Atmospheric Administration Woods Hole Sea Grant Program (Woods Hole Sea Grant, Woods Hole Oceanographic Institution, NA18OAR4170104, Project No. R/O-57; RJ, HB, and JKL), the Bureau of Ocean Energy Management (IA agreement M17PG0019; DNW, HB, and JKL) including a subaward via the National Marine Sanctuary Foundation (18-11-B-203), and a National Science Foundation Long-term Ecological Research grant for the Northeast US Shelf Ecosystem (OCE 1655686; RJ and JKL). JJS was funded by the National Science Foundation Graduate Research Fellowship program.
    Keywords: Gulf of Maine ; larval retention ; otolith microstructure ; particle tracking ; population connectivity ; sand lance
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  • 15
    Publication Date: 2022-08-31
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Fay, R., Hamel, S., van de Pol, M., Gaillard, J.-M., Yoccoz, N. G., Acker, P., Authier, M., Larue, B., Le Coeur, C., Macdonald, K. R., Nicol-Harper, A., Barbraud, C., Bonenfant, C., Van Vuren, D. H., Cam, E., Delord, K., Gamelon, M., Moiron, M., Pelletier, F., Rotella, J., Teplitsky, C., Visser, M. E., Wells, C. P., Wheelwright, N. T., Jenouvrier, S., & Saether, B.-E. Temporal correlations among demographic parameters are ubiquitous but highly variable across species. Ecology Letters, 25(7), (2022): 1640-1654, https://doi.org/10.1111/ele.14026.
    Description: Temporal correlations among demographic parameters can strongly influence population dynamics. Our empirical knowledge, however, is very limited regarding the direction and the magnitude of these correlations and how they vary among demographic parameters and species’ life histories. Here, we use long-term demographic data from 15 bird and mammal species with contrasting pace of life to quantify correlation patterns among five key demographic parameters: juvenile and adult survival, reproductive probability, reproductive success and productivity. Correlations among demographic parameters were ubiquitous, more frequently positive than negative, but strongly differed across species. Correlations did not markedly change along the slow-fast continuum of life histories, suggesting that they were more strongly driven by ecological than evolutionary factors. As positive temporal demographic correlations decrease the mean of the long-run population growth rate, the common practice of ignoring temporal correlations in population models could lead to the underestimation of extinction risks in most species.
    Description: This project was funded by the CNRS, including a long-term support by the OSU-OREME. Data collection for Weddell seals was supported by the National Science Foundation, Division of Polar Programs under grant number ANT-1640481 to J.J. Rotella, R.A. Garrott and D.B. Siniff and prior NSF Grants to R. A. Garrott, J. J. Rotella, D. B. Siniff and J. Ward Testa. Stéphanie Jenouvrier acknowledges the support of the NSF 1840058.
    Keywords: capture-recapture ; demographic correlation ; demography ; environmental stochasticity ; slow-fast continuum ; stochastic population dynamics ; temporal covariation
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  • 16
    Publication Date: 2022-10-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Kuehn, E., Clausen, D. S., Null, R. W., Metzger, B. M., Willis, A. D., & Ozpolat, B. D. Segment number threshold determines juvenile onset of germline cluster expansion in Platynereis dumerilii. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, (2021.): 1-16, https://doi.org/10.1002/jez.b.23100.
    Description: Development of sexual characters and generation of gametes are tightly coupled with growth. Platynereis dumerilii is a marine annelid that has been used to study germline development and gametogenesis. P. dumerilii has germ cell clusters found across the body in the juvenile worms, and the clusters eventually form the gametes. Like other segmented worms, P. dumerilii grows by adding new segments at its posterior end. The number of segments reflect the growth state of the worms and therefore is a useful and measurable growth state metric to study the growth-reproduction crosstalk. To understand how growth correlates with progression of gametogenesis, we investigated germline development across several developmental stages. We discovered a distinct transition period when worms increase the number of germline clusters at a particular segment number threshold. Additionally, we found that keeping worms short in segment number, by manipulating environmental conditions or via amputations, supported a segment number threshold requirement for germline development. Finally, we asked if these clusters in P. dumerilii play a role in regeneration (as similar free-roaming cells are observed in Hydra and planarian regeneration) and found that the clusters were not required for regeneration in P. dumerilii, suggesting a strictly germline nature. Overall, these molecular analyses suggest a previously unidentified developmental transition dependent on the growth state of juvenile P. dumerilii leading to substantially increased germline expansion.
    Description: Research reported in this publication was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R35GM138008 (to BDÖ) and R35GM133420 (to ADW) and Hibbitt Startup Funds (to BDÖ).
    Keywords: Annelida ; Critical size ; Developmental transition ; Gametogenesis ; Sexual reproduction
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  • 17
    Publication Date: 2022-10-27
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Stolp, Z. D., Kulkarni, M., Liu, Y., Zhu, C., Jalisi, A., Lin, S., Casadevall, A., Cunningham, K. W., Pineda, F. J., Teng, X., & Hardwick, J. M. Yeast cell death pathway requiring AP-3 vesicle trafficking leads to vacuole/lysosome membrane permeabilization. Cell Reports, 39(2), (2022): 110647, https://doi.org/10.1016/j.celrep.2022.110647.
    Description: Unicellular eukaryotes have been suggested as undergoing self-inflicted destruction. However, molecular details are sparse compared with the mechanisms of programmed/regulated cell death known for human cells and animal models. Here, we report a molecular cell death pathway in Saccharomyces cerevisiae leading to vacuole/lysosome membrane permeabilization. Following a transient cell death stimulus, yeast cells die slowly over several hours, consistent with an ongoing molecular dying process. A genome-wide screen for death-promoting factors identified all subunits of the AP-3 complex, a vesicle trafficking adapter known to transport and install newly synthesized proteins on the vacuole/lysosome membrane. To promote cell death, AP-3 requires its Arf1-GTPase-dependent vesicle trafficking function and the kinase Yck3, which is selectively transported to the vacuole membrane by AP-3. Video microscopy revealed a sequence of events where vacuole permeability precedes the loss of plasma membrane integrity. AP-3-dependent death appears to be conserved in the human pathogenic yeast Cryptococcus neoformans.
    Description: Funding sources: National Institutes of Health, United States grants AI144373 and NS127076 (J.M.H.), AI115016 and AI153414 (K.W.C.), and AI052733, AI152078, and HL059842 (A.C.); National Natural Science Foundation of China 31970550; and the Priority Academic Program Development of the Jiangsu Higher Education Institutes (X.T.).
    Keywords: Yeast ; Programmed cell death ; Vesicle trafficking ; AP-3 ; Vacuole ; Cryptococcus ; Yck3 ; Regulated cell death ; Lysosome ; Vacuolar membrane permeabilization
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  • 18
    Publication Date: 2022-10-27
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Sanders‐DeMott, R., Eagle, M., Kroeger, K., Wang, F., Brooks, T., Suttles, J., Nick, S., Mann, A., & Tang, J. Impoundment increases methane emissions in Phragmites‐invaded coastal wetlands. Global Change Biology, 28(15), (2022): 4539– 4557. https://doi.org/10.1111/gcb.16217.
    Description: Saline tidal wetlands are important sites of carbon sequestration and produce negligible methane (CH4) emissions due to regular inundation with sulfate-rich seawater. Yet, widespread management of coastal hydrology has restricted tidal exchange in vast areas of coastal wetlands. These ecosystems often undergo impoundment and freshening, which in turn cause vegetation shifts like invasion by Phragmites, that affect ecosystem carbon balance. Understanding controls and scaling of carbon exchange in these understudied ecosystems is critical for informing climate consequences of blue carbon restoration and/or management interventions. Here, we (1) examine how carbon fluxes vary across a salinity gradient (4–25 psu) in impounded and natural, tidally unrestricted Phragmites wetlands using static chambers and (2) probe drivers of carbon fluxes within an impounded coastal wetland using eddy covariance at the Herring River in Wellfleet, MA, United States. Freshening across the salinity gradient led to a 50-fold increase in CH4 emissions, but effects on carbon dioxide (CO2) were less pronounced with uptake generally enhanced in the fresher, impounded sites. The impounded wetland experienced little variation in water-table depth or salinity during the growing season and was a strong CO2 sink of −352 g CO2-C m−2 year−1 offset by CH4 emission of 11.4 g CH4-C m−2 year−1. Growing season CH4 flux was driven primarily by temperature. Methane flux exhibited a diurnal cycle with a night-time minimum that was not reflected in opaque chamber measurements. Therefore, we suggest accounting for the diurnal cycle of CH4 in Phragmites, for example by applying a scaling factor developed here of ~0.6 to mid-day chamber measurements. Taken together, these results suggest that although freshened, impounded wetlands can be strong carbon sinks, enhanced CH4 emission with freshening reduces net radiative balance. Restoration of tidal flow to impounded ecosystems could limit CH4 production and enhance their climate regulating benefits.
    Description: This project was supported by USGS-NPS Natural Resources Preservation Program #2021-07, U.S. Geological Survey Coastal & Marine Hazards and Resources Program and the USGS Land Change Science Program's LandCarbon program, and NOAA National Estuarine Research Reserve Science Collaborative NA14NOS4190145. R Sanders-DeMott was supported by a USGS Mendenhall Fellowship and partnership with Restore America's Estuaries.
    Keywords: Blue carbon ; Coastal wetland ; Dike ; Eddy covariance ; Impoundment ; Methane ; Net ecosystem exchange ; Phragmites ; Restoration ; Static chambers
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  • 19
    Publication Date: 2022-10-27
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Tsakalakis, I., Follows, M. J., Dutkiewicz, S., Follett, C. L., & Vallino, J. J. Diel light cycles affect phytoplankton competition in the global ocean. Global Ecology and Biogeography, 31(9), (2022): 1838-1849, https://doi.org/10.1111/geb.13562.
    Description: Aim Light, essential for photosynthesis, is present in two periodic cycles in nature: seasonal and diel. Although seasonality of light is typically resolved in ocean biogeochemical–ecosystem models because of its significance for seasonal succession and biogeography of phytoplankton, the diel light cycle is generally not resolved. The goal of this study is to demonstrate the impact of diel light cycles on phytoplankton competition and biogeography in the global ocean. Location Global ocean. Major taxa studied Phytoplankton. Methods We use a three-dimensional global ocean model and compare simulations of high temporal resolution with and without diel light cycles. The model simulates 15 phytoplankton types with different cell sizes, encompassing two broad ecological strategies: small cells with high nutrient affinity (gleaners) and larger cells with high maximal growth rate (opportunists). Both are grazed by zooplankton and limited by nitrogen, phosphorus and iron. Results Simulations show that diel cycles of light induce diel cycles in limiting nutrients in the global ocean. Diel nutrient cycles are associated with higher concentrations of limiting nutrients, by 100% at low latitudes (−40° to 40°), a process that increases the relative abundance of opportunists over gleaners. Size classes with the highest maximal growth rates from both gleaner and opportunist groups are favoured by diel light cycles. This mechanism weakens as latitude increases, because the effects of the seasonal cycle dominate over those of the diel cycle. Main conclusions Understanding the mechanisms that govern phytoplankton biogeography is crucial for predicting ocean ecosystem functioning and biogeochemical cycles. We show that the diel light cycle has a significant impact on phytoplankton competition and biogeography, indicating the need for understanding the role of diel processes in shaping macroecological patterns in the global ocean.
    Description: Simons Collaboration on Computational Biogeochemical Modeling of Marine Ecosystems supported M.J.F. and S.D. on CBIOMES grant #549931; C.L.F. on CBIOMES grants #827829 and #553242; and J.J.V. and I.T. on CBIOMES grant #549941. The National Science Foundation supported I.T. and J.J.V. on award #1558710 and J.J.V. on awards #1637630, #1655552 and #1841599.
    Keywords: Biogeography ; Diel light cycle ; Global ocean ; Modelling ; Nutrient cycles ; Phytoplankton ; Resource competition
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  • 20
    Publication Date: 2022-10-20
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Walter, J. A., Castorani, M. C. N., Bell, T. W., Sheppard, L. W., Cavanaugh, K. C., & Reuman, D. C. Tail-dependent spatial synchrony arises from nonlinear driver-response relationships. Ecology Letters, 25, (2022): 1189– 1201, https://doi.org/10.1111/ele.13991.
    Description: Spatial synchrony may be tail-dependent, that is, stronger when populations are abundant than scarce, or vice-versa. Here, ‘tail-dependent’ follows from distributions having a lower tail consisting of relatively low values and an upper tail of relatively high values. We present a general theory of how the distribution and correlation structure of an environmental driver translates into tail-dependent spatial synchrony through a non-linear response, and examine empirical evidence for theoretical predictions in giant kelp along the California coastline. In sheltered areas, kelp declines synchronously (lower-tail dependence) when waves are relatively intense, because waves below a certain height do little damage to kelp. Conversely, in exposed areas, kelp is synchronised primarily by periods of calmness that cause shared recovery (upper-tail dependence). We find evidence for geographies of tail dependence in synchrony, which helps structure regional population resilience: areas where population declines are asynchronous may be more resilient to disturbance because remnant populations facilitate reestablishment.
    Description: This research was supported by NSF-OCE awards 2023555, 2023523, 2140335, 2023474, and the James S McDonnell Foundation. This project used data developed through the Santa Barbara Coastal Long Term Ecological Research project, funded through NSF-OCE 1831937.
    Keywords: Copula ; Disturbance ; Giant kelp ; Macrocystis pyrifera ; Nutrients ; Stability ; Synchrony ; Waves
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  • 21
    Publication Date: 2022-10-20
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Rypkema, N., Schmidt, H., & Fischell, E. Synchronous-clock range-angle relative acoustic navigation: a unified approach to multi-AUV localization, command, control, and coordination. Journal of Field Robotics, 2(1), (2022): 774–806, https://doi.org/10.55417/fr.2022026.
    Description: This paper presents a scalable acoustic navigation approach for the unified command, control, and coordination of multiple autonomous underwater vehicles (AUVs). Existing multi-AUV operations typically achieve coordination manually by programming individual vehicles on the surface via radio communications, which becomes impractical with large vehicle numbers; or they require bi-directional intervehicle acoustic communications to achieve limited coordination when submerged, with limited scalability due to the physical properties of the acoustic channel. Our approach utilizes a single, periodically broadcasting beacon acting as a navigation reference for the group of AUVs, each of which carries a chip-scale atomic clock and fixed ultrashort baseline array of acoustic receivers. One-way travel-time from synchronized clocks and time-delays between signals received by each array element allow any number of vehicles within receive distance to determine range, angle, and thus determine their relative position to the beacon. The operator can command different vehicle behaviors by selecting between broadcast signals from a predetermined set, while coordination between AUVs is achieved without intervehicle communication by defining individual vehicle behaviors within the context of the group. Vehicle behaviors are designed within a beacon-centric moving frame of reference, allowing the operator to control the absolute position of the AUV group by repositioning the navigation beacon to survey the area of interest. Multiple deployments with a fleet of three miniature, low-cost SandShark AUVs performing closed-loop acoustic navigation in real-time provide experimental results validated against a secondary long-baseline positioning system, demonstrating the capabilities and robustness of our approach with real-world data.
    Description: This work was partially supported by the Office of Naval Research, the Defense Advanced Research Projects Agency, Lincoln Laboratory, and the Reuben F. and Elizabeth B. Richards Endowed Funds at WHOI.
    Keywords: Underwater robotics ; Navigation ; Multirobot systems ; Localization ; Marine robotics
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  • 22
    Publication Date: 2022-10-20
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Castorani, M. C. N., Bell, T. W., Walter, J. A., Reuman, D. C., Cavanaugh, K. C., & Sheppard, L. W. Disturbance and nutrients synchronise kelp forests across scales through interacting Moran effects. Ecology Letters, 25(8), (2022): 1854-1868, https://doi.org/10.1111/ele.14066.
    Description: Spatial synchrony is a ubiquitous and important feature of population dynamics, but many aspects of this phenomenon are not well understood. In particular, it is largely unknown how multiple environmental drivers interact to determine synchrony via Moran effects, and how these impacts vary across spatial and temporal scales. Using new wavelet statistical techniques, we characterised synchrony in populations of giant kelp Macrocystis pyrifera, a widely distributed marine foundation species, and related synchrony to variation in oceanographic conditions across 33 years (1987–2019) and 〉900 km of coastline in California, USA. We discovered that disturbance (storm-driven waves) and resources (seawater nutrients)—underpinned by climatic variability—act individually and interactively to produce synchrony in giant kelp across geography and timescales. Our findings demonstrate that understanding and predicting synchrony, and thus the regional stability of populations, relies on resolving the synergistic and antagonistic Moran effects of multiple environmental drivers acting on different timescales.
    Description: This study was funded by the U.S. National Science Foundation (NSF) through linked NSF-OCE awards 2023555, 2023523, 2140335, and 2023474 to M.C.N.C., K.C.C., T.W.B., and D.C.R., respectively. The research was initiated during a synthesis working group at the Long Term Ecological Research Network Office and National Center for Ecological Analysis and Synthesis funded under NSF-DEB award 1545288. D.C.R. and L.W.S. were also partly supported by NSF award 1714195, the McDonnell Foundation, and the California Department of Fish and Wildlife Delta Science Program. This project used data developed through the Santa Barbara Coastal Long Term Ecological Research project, funded through NSF-OCE award 1831937.
    Keywords: Coherence ; Disturbance ; Moran effect ; Nitrate ; North Pacific Gyre Oscillation ; Oceanography ; Population dynamics ; Remote sensing ; Spatial synchrony ; Wavelet transforms
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  • 23
    Publication Date: 2022-10-14
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Marshall, T., Granger, J., Casciotti, K. L., Dahnke, K., Emeis, K.-C., Marconi, D., McIlvin, M. R., Noble, A. E., Saito, M. A., Sigman, D. M., & Fawcett, S. E. The Angola Gyre is a hotspot of dinitrogen fixation in the South Atlantic Ocean. Communications Earth & Environment, 3(1), (2022): 151, https://doi.org/10.1038/s43247-022-00474-x.
    Description: Biological dinitrogen fixation is the major source of new nitrogen to marine systems and thus essential to the ocean’s biological pump. Constraining the distribution and global rate of dinitrogen fixation has proven challenging owing largely to uncertainty surrounding the controls thereon. Existing South Atlantic dinitrogen fixation rate estimates vary five-fold, with models attributing most dinitrogen fixation to the western basin. From hydrographic properties and nitrate isotope ratios, we show that the Angola Gyre in the eastern tropical South Atlantic supports the fixation of 1.4–5.4 Tg N.a−1, 28-108% of the existing (highly uncertain) estimates for the basin. Our observations contradict model diagnoses, revealing a substantial input of newly-fixed nitrogen to the tropical eastern basin and no dinitrogen fixation west of 7.5˚W. We propose that dinitrogen fixation in the South Atlantic occurs in hotspots controlled by the overlapping biogeography of excess phosphorus relative to nitrogen and bioavailable iron from margin sediments. Similar conditions may promote dinitrogen fixation in analogous ocean regions. Our analysis suggests that local iron availability causes the phosphorus-driven coupling of oceanic dinitrogen fixation to nitrogen loss to vary on a regional basis.
    Description: This work was supported by the South African National Research Foundation (114673 and 130826 to T.M., 115335, 116142 and 129320 to S.E.F.); the US National Science Foundation (CAREER award, OCE-1554474 to J.G., OCE-1736652 to D.M.S. and K.L.C., OCE-05-26277 to K.L.C.); the German Federal Agency for Education and Research (DAAD-SPACES 57371082 to T.M.); the Royal Society (FLAIR fellowship to S.E.F.); and the University of Cape Town (T.M., J.G., S.E.F.). The authors also recognize the support of the South African Department of Science and Innovation’s Biogeochemistry Research Infrastructure Platform (BIOGRIP).
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  • 24
    Publication Date: 2022-10-12
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Struve, T., Wilson, D., Hines, S., Adkins, J., & van de Flierdt, T. A deep Tasman outflow of Pacific waters during the last glacial period. Nature Communications, 13(1), (2022): 3763, https://doi.org/10.1038/s41467-022-31116-7.
    Description: The interoceanic exchange of water masses is modulated by flow through key oceanic choke points in the Drake Passage, the Indonesian Seas, south of Africa, and south of Tasmania. Here, we use the neodymium isotope signature (εNd) of cold-water coral skeletons from intermediate depths (1460‒1689 m) to trace circulation changes south of Tasmania during the last glacial period. The key feature of our dataset is a long-term trend towards radiogenic εNd values of ~−4.6 during the Last Glacial Maximum and Heinrich Stadial 1, which are clearly distinct from contemporaneous Southern Ocean εNd of ~−7. When combined with previously published radiocarbon data from the same corals, our results indicate that a unique radiogenic and young water mass was present during this time. This scenario can be explained by a more vigorous Pacific overturning circulation that supported a deeper outflow of Pacific waters, including North Pacific Intermediate Water, through the Tasman Sea.
    Description: The authors acknowledge financial support from the Grantham Institute of Climate Change and the Environment (T.v.d.F. and T.S.), the Ministry for Science and Culture of the State of Lower Saxony (T.S.), Marie Curie Reintegration grant IRG 230828 (T.v.d.F.), Leverhulme Trust grant RPG-398 (T.v.d.F.), Natural Environment Research Council grants NE/F016751/1 (T.v.d.F.), NE/N001141/1 (T.v.d.F. and D.J.W.), and NE/T011440/1 (D.J.W.), and National Science Foundation grant OCE-1503129 (J.F.A. and S.K.V.H.). Open Access funding is enabled by the DFG open access publication fund and the Carl von Ossietzky University Oldenburg.
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  • 25
    Publication Date: 2022-10-20
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Billings, G., Walter, M., Pizarro, O., Johnson-Roberson, M., & Camilli, R. Towards automated sample collection and return in extreme underwater environments. Journal of Field Robotics, 2(1), (2022): 1351–1385, https://doi.org/10.55417/fr.2022045.
    Description: In this report, we present the system design, operational strategy, and results of coordinated multivehicle field demonstrations of autonomous marine robotic technologies in search-for-life missions within the Pacific shelf margin of Costa Rica and the Santorini-Kolumbo caldera complex, which serve as analogs to environments that may exist in oceans beyond Earth. This report focuses on the automation of remotely operated vehicle (ROV) manipulator operations for targeted biological sample-collection-and-return from the seafloor. In the context of future extraterrestrial exploration missions to ocean worlds, an ROV is an analog to a planetary lander, which must be capable of high-level autonomy. Our field trials involve two underwater vehicles, the SuBastian ROV and the Nereid Under Ice (NUI) hybrid ROV for mixed initiative (i.e., teleoperated or autonomous) missions, both equipped seven-degrees-of-freedom hydraulic manipulators. We describe an adaptable, hardware-independent computer vision architecture that enables high-level automated manipulation. The vision system provides a three-dimensional understanding of the workspace to inform manipulator motion planning in complex unstructured environments. We demonstrate the effectiveness of the vision system and control framework through field trials in increasingly challenging environments, including the automated collection and return of biological samples from within the active undersea volcano Kolumbo. Based on our experiences in the field, we discuss the performance of our system and identify promising directions for future research.
    Description: This work was funded under a NASA PSTAR grant, number NNX16AL08G, and by the National Science Foundation under grants IIS-1830660 and IIS-1830500. The authors would like to thank the Costa Rican Ministry of Environment and Energy and National System of Conservation Areas for permitting research operations at the Costa Rican shelf margin, and the Schmidt Ocean Institute (including the captain and crew of the R/V Falkor and ROV SuBastian) for their generous support and making the FK181210 expedition safe and highly successful. Additionally, the authors would like to thank the Greek Ministry of Foreign Affairs for permitting the 2019 Kolumbo Expedition to the Kolumbo and Santorini calderas, as well as Prof. Evi Nomikou and Dr. Aggelos Mallios for their expert guidance and tireless contributions to the expedition.
    Keywords: Underwater robotics ; Mobile manipulation ; Marine robotics ; Exploration
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  • 26
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Sil, S., Gangopadhyay, A., Gawarkiewicz, G., & Pramanik, S. Shifting seasonality of cyclones and western boundary current interactions in Bay of Bengal as observed during Amphan and Fani. Scientific Reports, 11(1), (2021): 22052 https://doi.org/10.1038/s41598-021-01607-6.
    Description: In recent years, the seasonal patterns of Tropical Cyclones (TC) in the Bay of Bengal have been shifting. While tropical depressions have been common in March–May (spring), they typically have been relatively weaker than the TCs during October–December. Here we show that the spatial pattern of recent warming trends during the last two decades in the southwestern Bay has allowed for stronger springtime pre-monsoon cyclones such as Amphan (May 2020, Super Cyclone) and Fani (April–May 2019, Extremely Severe Cyclone). The tracks of the pre-monsoon cyclones shifted westward, concurrent with an increasing rate of warming. This shift allowed both Fani and Amphan tracks to cross the northeastward warm Western Boundary Current (WBC) and associated warm anti-cyclonic eddies, while the weaker Viyaru (April 2013, Cyclonic Storm) did not interact with the WBC. A quantitative model linking the available along-track heat potential to cyclone’s intensity is developed to understand the impact of the WBC on cyclone intensification. The influence of the warming WBC and associated anti-cyclonic eddies will likely result in much stronger springtime TCs becoming relatively common in the future.
    Description: The authors gratefully acknowledge the financial and infrastructural support from the Indian Institute of Technology Bhubaneswar to carry out this research. SS acknowledges the financial assistance from Science and Engineering Research Board (SERB), Government of India (Grant No. CRG/2019/005842). All the figures are prepared using MATLAB. AG and SS appreciate the support of SERB's VAJRA Faculty Scheme (VJR/2018/000108) for the initiation of this collaborative work between SMAST and IITBBS. AG also acknowledges partial support from NSF (OCE 1851242) in completing this manuscript. GG was supported by a Grant from the Office of Naval Research as part of the Task Force Ocean initiative.
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  • 27
    Publication Date: 2022-05-27
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Jenouvrier, S., Long, M. C., Coste, C. F. D., Holland, M., Gamelon, M., Yoccoz, N., & Saether, B.-E. Detecting climate signals in populations across life histories. Global Change Biology, 28, (2022): 2236– 2258, https://doi.org/10.1111/gcb.16041.
    Description: Climate impacts are not always easily discerned in wild populations as detecting climate change signals in populations is challenged by stochastic noise associated with natural climate variability, variability in biotic and abiotic processes, and observation error in demographic rates. Detection of the impact of climate change on populations requires making a formal distinction between signals in the population associated with long-term climate trends from those generated by stochastic noise. The time of emergence (ToE) identifies when the signal of anthropogenic climate change can be quantitatively distinguished from natural climate variability. This concept has been applied extensively in the climate sciences, but has not been explored in the context of population dynamics. Here, we outline an approach to detecting climate-driven signals in populations based on an assessment of when climate change drives population dynamics beyond the envelope characteristic of stochastic variations in an unperturbed state. Specifically, we present a theoretical assessment of the time of emergence of climate-driven signals in population dynamics (ToEpop). We identify the dependence of (ToEpop)on the magnitude of both trends and variability in climate and also explore the effect of intrinsic demographic controls on (ToEpop). We demonstrate that different life histories (fast species vs. slow species), demographic processes (survival, reproduction), and the relationships between climate and demographic rates yield population dynamics that filter climate trends and variability differently. We illustrate empirically how to detect the point in time when anthropogenic signals in populations emerge from stochastic noise for a species threatened by climate change: the emperor penguin. Finally, we propose six testable hypotheses and a road map for future research.
    Description: We acknowledge the support of NASA 80NSSC20K1289 to SJ, ML, and MH; NSF OPP 1744794 to SJ and NSF OPP 2037561 to SJ and MH.
    Keywords: climate change ; emperor penguin ; life histories ; population trend ; population variability ; signal to noise ; time of emergence
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  • 28
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Mayorova, T. D., Hammar, K., Jung, J. H., Aronova, M. A., Zhang, G., Winters, C. A., Reese, T. S., & Smith, C. L. Placozoan fiber cells: mediators of innate immunity and participants in wound healing. Scientific Reports, 11(1), (2021): 23343, https://doi.org/10.1038/s41598-021-02735-9.
    Description: Placozoa is a phylum of non-bilaterian marine animals. These small, flat organisms adhere to the substrate via their densely ciliated ventral epithelium, which mediates mucociliary locomotion and nutrient uptake. They have only six morphological cell types, including one, fiber cells, for which functional data is lacking. Fiber cells are non-epithelial cells with multiple processes. We used electron and light microscopic approaches to unravel the roles of fiber cells in Trichoplax adhaerens, a representative member of the phylum. Three-dimensional reconstructions of serial sections of Trichoplax showed that each fiber cell is in contact with several other cells. Examination of fiber cells in thin sections and observations of live dissociated fiber cells demonstrated that they phagocytose cell debris and bacteria. In situ hybridization confirmed that fiber cells express genes involved in phagocytic activity. Fiber cells also are involved in wound healing as evidenced from microsurgery experiments. Based on these observations we conclude that fiber cells are multi-purpose macrophage-like cells. Macrophage-like cells have been described in Porifera, Ctenophora, and Cnidaria and are widespread among Bilateria, but our study is the first to show that Placozoa possesses this cell type. The phylogenetic distribution of macrophage-like cells suggests that they appeared early in metazoan evolution.
    Description: Open Access funding provided by the National Institutes of Health (NIH).
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  • 29
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Brun, N. R., Salanga, M. C., Mora-Zamorano, F. X., Lamb, D. C., Goldstone, J. V., & Stegeman, J. J. Orphan cytochrome P450 20a1 CRISPR/Cas9 mutants and neurobehavioral phenotypes in zebrafish. Scientific Reports, 11(1), (2021): 23892, https://doi.org/10.1038/s41598-021-03068-3.
    Description: Orphan cytochrome P450 (CYP) enzymes are those for which biological substrates and function(s) are unknown. Cytochrome P450 20A1 (CYP20A1) is the last human orphan P450 enzyme, and orthologs occur as single genes in every vertebrate genome sequenced to date. The occurrence of high levels of CYP20A1 transcripts in human substantia nigra and hippocampus and abundant maternal transcripts in zebrafish eggs strongly suggest roles both in the brain and during early embryonic development. Patients with chromosome 2 microdeletions including CYP20A1 show hyperactivity and bouts of anxiety, among other conditions. Here, we created zebrafish cyp20a1 mutants using CRISPR/Cas9, providing vertebrate models with which to study the role of CYP20A1 in behavior and other neurodevelopmental functions. The homozygous cyp20a1 null mutants exhibited significant behavioral differences from wild-type zebrafish, both in larval and adult animals. Larval cyp20a1-/- mutants exhibited a strong increase in light-simulated movement (i.e., light–dark assay), which was interpreted as hyperactivity. Further, the larvae exhibited mild hypoactivity during the adaptation period of the optomotor assays. Adult cyp20a1 null fish showed a pronounced delay in adapting to new environments, which is consistent with an anxiety paradigm. Taken together with our earlier morpholino cyp20a1 knockdown results, the results described herein suggest that the orphan CYP20A1 has a neurophysiological role.
    Description: These studies were supported in part by the Boston University Superfund Research Program NIH 5P42ES007381 (MCS, NRB, FXM, JVG, JJS), the Woods Hole Center for Oceans and Human Health (NIH: P01ES021923 and P01ES028938; NSF: OCE-1314642 and OCE-1840381; NRB and JJS), and EBI/EMBL Medakatox NIEHS R01ES029917 (JVG). DCL was funded by a UK-US Fulbright Scholarship.
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  • 30
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Beman, J. M., Vargas, S. M., Wilson, J. M., Perez-Coronel, E., Karolewski, J. S., Vazquez, S., Yu, A., Cairo, A. E., White, M. E., Koester, I., Aluwihare, L. I., & Wankel, S. D. Substantial oxygen consumption by aerobic nitrite oxidation in oceanic oxygen minimum zones. Nature Communications, 12(1), (2021): 7043, https://doi.org/10.1038/s41467-021-27381-7.
    Description: Oceanic oxygen minimum zones (OMZs) are globally significant sites of biogeochemical cycling where microorganisms deplete dissolved oxygen (DO) to concentrations 〈20 µM. Amid intense competition for DO in these metabolically challenging environments, aerobic nitrite oxidation may consume significant amounts of DO and help maintain low DO concentrations, but this remains unquantified. Using parallel measurements of oxygen consumption rates and 15N-nitrite oxidation rates applied to both water column profiles and oxygen manipulation experiments, we show that the contribution of nitrite oxidation to overall DO consumption systematically increases as DO declines below 2 µM. Nitrite oxidation can account for all DO consumption only under DO concentrations 〈393 nM found in and below the secondary chlorophyll maximum. These patterns are consistent across sampling stations and experiments, reflecting coupling between nitrate reduction and nitrite-oxidizing Nitrospina with high oxygen affinity (based on isotopic and omic data). Collectively our results demonstrate that nitrite oxidation plays a pivotal role in the maintenance and biogeochemical dynamics of OMZs.
    Description: This work was supported by NSF CAREER Grant OCE-1555375 to J.M.B. Metagenome sequencing was supported by the UCMEXUS-CONACyT Collaborative Grants Program (joint awards to J.M.B. and José García Maldonado).
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  • 31
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Remple, K. L., Silbiger, N. J., Quinlan, Z. A., Fox, M. D., Kelly, L. W., Donahue, M. J., & Nelson, C. E. Coral reef biofilm bacterial diversity and successional trajectories are structured by reef benthic organisms and shift under chronic nutrient enrichment. Npj Biofilms and Microbiomes, 7(1), (2021): 84, https://doi.org/10.1038/s41522-021-00252-1.
    Description: Work on marine biofilms has primarily focused on host-associated habitats for their roles in larval recruitment and disease dynamics; little is known about the factors regulating the composition of reef environmental biofilms. To contrast the roles of succession, benthic communities and nutrients in structuring marine biofilms, we surveyed bacteria communities in biofilms through a six-week succession in aquaria containing macroalgae, coral, or reef sand factorially crossed with three levels of continuous nutrient enrichment. Our findings demonstrate how biofilm successional trajectories diverge from temporal dynamics of the bacterioplankton and how biofilms are structured by the surrounding benthic organisms and nutrient enrichment. We identify a suite of biofilm-associated bacteria linked with the orthogonal influences of corals, algae and nutrients and distinct from the overlying water. Our results provide a comprehensive characterization of marine biofilm successional dynamics and contextualize the impact of widespread changes in reef community composition and nutrient pollution on biofilm community structure.
    Description: This work was supported through grants from the National Science Foundation for Biological Oceanography (1923877 to C.E.N. and M.J.D., 1949033 to C.E.N. and 2118687 to L.W.K., and 1924281 to N.J.S.) and the National Fish and Wildlife Foundation (grant no. 44447 to C.E.N.). This paper is funded in part by the National Oceanic and Atmospheric Administration, Project A/AS-1, which is sponsored by the University of Hawaii Sea Grant College Program, SOEST, under Institutional Grant No. NA18OAR4170076 from NOAA Office of Sea Grant, Department of Commerce. This is CSUN marine biology contribution #365, UH Sea Grant contribution UNIHI-SEAGRANT-JC-21-06, and UH SOEST contribution 11435.
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  • 32
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in James, B. D., Kimmins, K. M., Nguyen, M.-T., Lausch, A. J., & Sone, E. D. Attachment of zebra and quagga mussel adhesive plaques to diverse substrates. Scientific Reports, 11(1), (2021): 23998, https://doi.org/10.1038/s41598-021-03227-6.
    Description: Like marine mussels, freshwater zebra and quagga mussels adhere via the byssus, a proteinaceous attachment apparatus. Attachment to various surfaces allows these invasive mussels to rapidly spread, however the adhesion mechanism is not fully understood. While marine mussel adhesion mechanics has been studied at the individual byssal-strand level, freshwater mussel adhesion has only been characterized through whole-mussel detachment, without direct interspecies comparisons on different substrates. Here, adhesive strength of individual quagga and zebra mussel byssal plaques were measured on smooth substrates with varying hydrophobicity—glass, PVC, and PDMS. With increased hydrophobicity of substrates, adhesive failures occurred more frequently, and mussel adhesion strength decreased. A new failure mode termed 'footprint failure' was identified, where failure appeared to be adhesive macroscopically, but a microscopic residue remained on the surface. Zebra mussels adhered stronger and more frequently on PDMS than quagga mussels. While their adhesion strengths were similar on PVC, there were differences in the failure mode and the plaque-substrate interface ultrastructure. Comparisons with previous marine mussel studies demonstrated that freshwater mussels adhere with comparable strength despite known differences in protein composition. An improved understanding of freshwater mussel adhesion mechanics may help explain spreading dynamics and will be important in developing effective antifouling surfaces.
    Description: This work was supported by Discovery grant (#342455) to EDS from the National Sciences and Engineering Research Council (NSERC) of Canada (RGPIN-2019-06210).
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  • 33
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Johnson, M. D., Swaminathan, S. D., Nixon, E. N., Paul, V. J., & Altieri, A. H. Differential susceptibility of reef-building corals to deoxygenation reveals remarkable hypoxia tolerance. Scientific Reports, 11(1), (2021): 23168, https://doi.org/10.1038/s41598-021-01078-9.
    Description: Ocean deoxygenation threatens the persistence of coastal ecosystems worldwide. Despite an increasing awareness that coastal deoxygenation impacts tropical habitats, there remains a paucity of empirical data on the effects of oxygen limitation on reef-building corals. To address this knowledge gap, we conducted laboratory experiments with ecologically important Caribbean corals Acropora cervicornis and Orbicella faveolata. We tested the effects of continuous exposure to conditions ranging from extreme deoxygenation to normoxia (~ 1.0 to 6.25 mg L−1 dissolved oxygen) on coral bleaching, photophysiology, and survival. Coral species demonstrated markedly different temporal resistance to deoxygenation, and within a species there were minimal genotype-specific treatment effects. Acropora cervicornis suffered tissue loss and mortality within a day of exposure to severe deoxygenation (~ 1.0 mg L−1), whereas O. faveolata remained unaffected after 11 days of continuous exposure to 1.0 mg L−1. Intermediate deoxygenation treatments (~ 2.25 mg L−1, ~ 4.25 mg L−1) elicited minimal responses in both species, indicating a low oxygen threshold for coral mortality and coral resilience to oxygen concentrations that are lethal for other marine organisms. These findings demonstrate the potential for variability in species-specific hypoxia thresholds, which has important implications for our ability to predict how coral reefs may be affected as ocean deoxygenation intensifies. With deoxygenation emerging as a critical threat to tropical habitats, there is an urgent need to incorporate deoxygenation into coral reef research, management, and action plans to facilitate better stewardship of coral reefs in an era of rapid environmental change.
    Description: This research was funded by an award from the National Oceanic and Atmospheric Administration’s National Centers for Coastal Ocean Science Competitive Research Program to AA, MJ, and VP (NA18NOS4780170) through the University of Florida. MJ was funded by postdoctoral fellow awards from the Smithsonian Institution's Marine Global Earth Observatory (MarineGEO), the Smithsonian Marine Station, and Woods Hole Oceanographic Institution. This material is based on work supported by the National Science Foundation Graduate Research Fellowship under Grant No. DGE-1842473. This is contribution 259 from the Coastal Hypoxia Research Program, 93 from the Smithsonian’s MarineGEO and Tennenbaum Marine Observatories Network, and 1167 from the Smithsonian Marine Station at Fort Pierce.
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  • 34
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Tyne, R. L., Barry, P. H., Lawson, M., Byrne, D. J., Warr, O., Xie, H., Hillegonds, D. J., Formolo, M., Summers, Z. M., Skinner, B., Eiler, J. M., & Ballentine, C. J. Rapid microbial methanogenesis during CO2 storage in hydrocarbon reservoirs. Nature, 600(7890), (2021): 670-674, https://doi.org/10.1038/s41586-021-04153-3.
    Description: Carbon capture and storage (CCS) is a key technology to mitigate the environmental impact of carbon dioxide (CO2) emissions. An understanding of the potential trapping and storage mechanisms is required to provide confidence in safe and secure CO2 geological sequestration1,2. Depleted hydrocarbon reservoirs have substantial CO2 storage potential1,3, and numerous hydrocarbon reservoirs have undergone CO2 injection as a means of enhanced oil recovery (CO2-EOR), providing an opportunity to evaluate the (bio)geochemical behaviour of injected carbon. Here we present noble gas, stable isotope, clumped isotope and gene-sequencing analyses from a CO2-EOR project in the Olla Field (Louisiana, USA). We show that microbial methanogenesis converted as much as 13–19% of the injected CO2 to methane (CH4) and up to an additional 74% of CO2 was dissolved in the groundwater. We calculate an in situ microbial methanogenesis rate from within a natural system of 73–109 millimoles of CH4 per cubic metre (standard temperature and pressure) per year for the Olla Field. Similar geochemical trends in both injected and natural CO2 fields suggest that microbial methanogenesis may be an important subsurface sink of CO2 globally. For CO2 sequestration sites within the environmental window for microbial methanogenesis, conversion to CH4 should be considered in site selection.
    Description: R.L.T. was supported by a Natural Environment Research Council studentship (grant reference NE/L002612/1). C.J.B. and P.H.B. acknowledge A. Regberg and B. Meurer for their support of the project and help with sample collection. C.J.B. was part supported by an Earth4D CIFAR fellowship. P.H.B. was supported by NSF awards 1923915 and 2015789. O.W. was supported by Natural Sciences and Engineering Research Council of Canada Discovery and Accelerator grants awarded to the Sherwood Lollar research group and acknowledges B. Sherwood Lollar’s support for the project. Z.M.S. acknowledges J. Biddle and G. Christman for their help in generating the microbial data.
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  • 35
    Publication Date: 2022-08-03
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Schmidbaur, H., Kawaguchi, A., Clarence, T., Fu, X., Hoang, O. P., Zimmermann, B., Ritschard, E. A., Weissenbacher, A., Foster, J. S., Nyholm, S., Bates, P. A., Albertin, C. B., Tanaka, E., & Simakov, O. Emergence of novel cephalopod gene regulation and expression through large-scale genome reorganization. Nature Communications, 13(1), (2022): 2172, https://doi.org/10.1038/s41467-022-29694-7.
    Description: Coleoid cephalopods (squid, cuttlefish, octopus) have the largest nervous system among invertebrates that together with many lineage-specific morphological traits enables complex behaviors. The genomic basis underlying these innovations remains unknown. Using comparative and functional genomics in the model squid Euprymna scolopes, we reveal the unique genomic, topological, and regulatory organization of cephalopod genomes. We show that coleoid cephalopod genomes have been extensively restructured compared to other animals, leading to the emergence of hundreds of tightly linked and evolutionary unique gene clusters (microsyntenies). Such novel microsyntenies correspond to topological compartments with a distinct regulatory structure and contribute to complex expression patterns. In particular, we identify a set of microsyntenies associated with cephalopod innovations (MACIs) broadly enriched in cephalopod nervous system expression. We posit that the emergence of MACIs was instrumental to cephalopod nervous system evolution and propose that microsyntenic profiling will be central to understanding cephalopod innovations.
    Description: H.S., O.P.H., E.R., and O.S. were supported by the Austrian Science Fund (FWF) grant P30686-B29. O.S. was supported by Whitman Center Early Career Fellowship (Frank R. Lillie Quasi-Endowment Fund, L. & A. Colwin Summer Research Fellowship, Bell Research Award in Tissue Engineering). H.S. was supported by the short-term grant abroad (KWA) of the University of Vienna. H.S. and O.S. were supported by the University of Chicago/Vienna Strategic Partnership Programme Mobility Grant. A.K. was supported by the JSPS Postdoctoral Fellowship for Overseas Researchers program from Japan. C.B.A. was supported by the Hibbitt Early Career Fellowship. Eggs and paralarvae of E. scolopes were generated in part by support by the NASA Space Biology 80NSSC18K1465 awarded to J.S.F. S.V.N. was supported by the National Science Foundation IOS-1557914. This work was supported by the Francis Crick Institute, which receives its core funding from Cancer Research UK (FC0001003), the UK Medical Research Council (FC001003), and the Wellcome Trust (FC001003).
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  • 36
    Publication Date: 2022-07-26
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Carson, M., Doberneck, D., Hart, Z., Kelsey, H., Pierce, J., Porter, D., Richlen, M., Schandera, L., & Triezenberg, H. A strategic framework for community engagement in oceans and human health, Community Science, 1(1), (2022): e2022CSJ000001, https://doi.org/10.1029/2022csj000001.
    Description: Over the past two decades, scientific research on the connections between the health and resilience of marine ecosystems and human health, well-being, and community prosperity has expanded and evolved into a distinct “metadiscipline” known as Oceans and Human Health (OHH), recognized by the scientific community as well as policy makers. OHH goals are diverse and seek to improve public health outcomes, promote sustainable use of aquatic systems and resources, and strengthen community resilience. OHH research has historically included some level of community outreach and partner involvement; however, the increasing disruption of aquatic environments and urgency of public health impacts calls for a more systematic approach to effectively identify and engage with community partners to achieve project goals and outcomes. Herein, we present a strategic framework developed collaboratively by community engagement personnel from the four recently established U.S. Centers for Oceans and Human Health (COHH). This framework supports researchers in defining levels of community engagement and in aligning partners, purpose, activities, and approaches intentionally in their community engagement efforts. Specifically, we describe: (a) a framework for a range of outreach and engagement approaches; (b) the need for identifying partners, purpose, activities, and approaches; and (c) the importance of making intentional alignment among them. Misalignment across these dimensions may lead to wasting time or resources, eroding public trust, or failing to achieve intended outcomes. We illustrate the framework with examples from current COHH case studies and conclude with future directions for strategic community engagement in OHH and other environmental health contexts.
    Description: This publication was prepared by Heather Triezenberg and the team under award NA180AR4170102 from the National Oceanic and Atmospheric Administration, U.S. Department of Commerce through the Regents of the University of Michigan, and supported by funding from the NIH (1P01ES028939-01) and the NSF (1840715) to the Bowling Green State University Great Lakes Center for Fresh Waters and Human Health. Funding for M. L. Richlen was provided by the NSF (OCE1840381) and NIH (1P01-ES028938-01) through the Woods Hole Center for Oceans and Human Health. Research at the Center for Oceans and Human Health and Climate Change Interactions (OHHC2I) at the University of South Carolina is supported by the NIH Award Number P01ES028942, granted to Principal Investigators Geoffrey Scott and Paul Sandifer. M. A. Carson, Z. Hart, H. Kelsey, D. E. Porter, and L. Schandera are Community Engagement Core investigators at this Center. Funding for J. Pierce is provided by the NSF (grant number OCE-1841811) and the NIH (P01ES028949) through the Greater Caribbean Center for Ciguatera Research at the Florida Gulf Coast University.
    Keywords: harmful algal blooms ; human health ; pollutants ; ocean health
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  • 37
    Publication Date: 2022-08-04
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Caudron, C., Vandemeulebrouck, J., & Sohn, R. A. Turbulence-induced bubble nucleation in hydrothermal fluids beneath Yellowstone Lake. Communications Earth & Environment, 3(1), (2022): 103, https://doi.org/10.1038/s43247-022-00417-6.
    Description: Volcanic systems generate large amounts of gas, and understanding gas fluxes is a fundamental aspect of volcanology and hazard mitigation. Volcanic gases can be challenging to measure, but acoustic methods hold promise in underwater environments because gas bubbles are powerful sound sources. We deployed an acoustic system to study the nature of gas discharge at a large (~30 MW) thermal field on the floor of Yellowstone Lake, which has experienced numerous hydrothermal explosions since the last glaciation (~13.4 ka). We find that small (〈10 Pa) turbulent flow instabilities trigger the nucleation of CO2 bubbles in the saturated fluids. The observation of CO2 bubbles nucleating in hydrothermal fluids due to small pressure perturbations informs our understanding of hydrothermal explosions in Yellowstone Lake, and demonstrates that acoustic data in underwater environments can provide insight into the stability of gas-rich systems, as well as gas fluxes.
    Description: This research was supported by the National Science Foundation grant EAR-1516361 to R.A.S. All work in Yellowstone National Park was completed under an authorized research permit (YELL-2018-SCI-7018). We also acknowledge the IRGA 2021 Volquan project (funded by Université Grenoble Alpes) and Thomas Jefferson Fund Face Foundation (project TJF20_009 ‘Quantifying underwater volcano degassing using novel seismo-acoustic approaches’).
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  • 38
    Publication Date: 2022-08-15
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Sievert, S. M., Buehring, S., Gulmann, L. K., Hinrichs, K.-U., Ristova, P. P., & Gomez-Saez, G. Fluid flow stimulates chemoautotrophy in hydrothermally influenced coastal sediments. Communications Earth & Environment, 3(1), (2022): 96, https://doi.org/10.1038/s43247-022-00426-5.
    Description: Hydrothermalism in coastal sediments strongly impacts biogeochemical processes and supports chemoautotrophy. Yet, the effect of fluid flow on microbial community composition and rates of chemoautotrophic production is unknown because rate measurements under natural conditions are difficult, impeding an assessment of the importance of these systems. Here, in situ incubations controlling fluid flow along a transect of three geochemically distinct locations at a shallow-water hydrothermal system off Milos (Greece) show that Campylobacteria dominated chemoautotrophy in the presence of fluid flow. Based on injected 13C-labelled dissolved inorganic carbon and its incorporation into fatty acids, we constrained carbon fixation to be as high as 12 µmol C cm−3 d−1, corresponding to areal rates up to 10-times higher than previously reported for coastal sediments, and showed the importance of fluid flow for supplying the necessary substrates to support chemoautotrophy. Without flow, rates were substantially lower and microbial community composition markedly shifted. Our results highlight the importance of fluid flow in shaping the composition and activity of microbial communities of shallow-water hydrothermal vents, identifying them as hotspots of microbial productivity.
    Description: Open Access funding enabled and organized by Projekt DEAL.
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  • 39
    Publication Date: 2022-08-15
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Sufke, F., Gutjahr, M., Keigwin, L. D., Reilly, B., Giosan, L., & Lippold, J. Arctic drainage of Laurentide Ice Sheet meltwater throughout the past 14,700 years. Communications Earth & Environment, 3(1), (2022): 98, https://doi.org/10.1038/s43247-022-00428-3.
    Description: During the last deglaciation substantial volumes of meltwater from the decaying Laurentide Ice Sheet were supplied to the Arctic, Gulf of Mexico and North Atlantic along different drainage routes, sometimes as catastrophic flood events. These events are suggested to have impacted global climate, for example initiating the Younger Dryas cold period. Here we analyze the authigenic Pb isotopic composition of sediments in front of the Arctic Mackenzie Delta, a sensitive tracer for elevated freshwater runoff of the retreating Laurentide Ice Sheet. Our data reveal continuous meltwater supply to the Arctic along the Mackenzie River since the onset of the Bølling–Allerød. The strongest Lake Agassiz outflow event is observed at the end of the Bølling–Allerød close to the onset of the Younger Dryas. In context of deglacial North American runoff records from the southern and eastern outlets, our findings provide a detailed reconstruction of the deglacial drainage chronology of the disintegrating Laurentide Ice Sheet.
    Description: Open Access funding enabled and organized by Projekt DEAL.
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  • 40
    Publication Date: 2022-08-30
    Description: Most tropical corals live in symbiosis with Symbiodiniaceae algae whose photosynthetic production of oxygen (O2) may lead to excess O2 in the diffusive boundary layer (DBL) above the coral surface. When flow is low, cilia-induced mixing of the coral DBL is vital to remove excess O2 and prevent oxidative stress that may lead to coral bleaching and mortality. Here, we combined particle image velocimetry using O2-sensitive nanoparticles (sensPIV) with chlorophyll (Chla)-sensitive hyperspectral imaging to visualize the microscale distribution and dynamics of ciliary flows and O2 in the coral DBL in relation to the distribution of Symbiodiniaceae Chla in the tissue of the reef building coral, Porites lutea. Curiously, we found an inverse relation between O2 in the DBL and Chla in the underlying tissue, with patches of high O2 in the DBL above low Chla in the underlying tissue surrounding the polyp mouth areas and pockets of low O2 concentrations in the DBL above high Chla in the coenosarc tissue connecting neighboring polyps. The spatial segregation of Chla and O2 is related to ciliary-induced flows, causing a lateral redistribution of O2 in the DBL. In a 2D transport-reaction model of the coral DBL, we show that the enhanced O2 transport allocates parts of the O2 surplus to areas containing less chla, which minimizes oxidative stress. Cilary flows thus confer a spatially complex mass transfer in the coral DBL, which may play an important role in mitigating oxidative stress and bleaching in corals.
    Repository Name: EPIC Alfred Wegener Institut
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  • 41
    Publication Date: 2022-08-19
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Albertin, C. B., Medina-Ruiz, S., Mitros, T., Schmidbaur, H., Sanchez, G., Wang, Z. Y., Grimwood, J., Rosenthal, J. J. C., Ragsdale, C. W., Simakov, O., & Rokhsar, D. S. Genome and transcriptome mechanisms driving cephalopod evolution. Nature Communications, 13(1), (2022): 2427, https://doi.org/10.1038/s41467-022-29748-w.
    Description: Cephalopods are known for their large nervous systems, complex behaviors and morphological innovations. To investigate the genomic underpinnings of these features, we assembled the chromosomes of the Boston market squid, Doryteuthis (Loligo) pealeii, and the California two-spot octopus, Octopus bimaculoides, and compared them with those of the Hawaiian bobtail squid, Euprymna scolopes. The genomes of the soft-bodied (coleoid) cephalopods are highly rearranged relative to other extant molluscs, indicating an intense, early burst of genome restructuring. The coleoid genomes feature multi-megabase, tandem arrays of genes associated with brain development and cephalopod-specific innovations. We find that a known coleoid hallmark, extensive A-to-I mRNA editing, displays two fundamentally distinct patterns: one exclusive to the nervous system and concentrated in genic sequences, the other widespread and directed toward repetitive elements. We conclude that coleoid novelty is mediated in part by substantial genome reorganization, gene family expansion, and tissue-dependent mRNA editing.
    Description: We thank the Marine Resources Center and the Cephalopod program at the Marine Biological Laboratory for supplying D. pealeii, R. Hanlon for the image in Fig. 1a, R. Hanlon and S. Senft for help with tissue dissection, Dr. Chuck Winkler for supplying O. bimaculoides, B. Burford and W. Gilly for assistance with D. opalescens collection, and the Vienna Zoo (Tiergarten Schönbrunn), particularly R. Halbauer, A. Weissenbacher, and the aquarist team for E. scolopes husbandry. Computation was done using the Life Science Cluster at the University of Vienna. This project began with generous funding from the Grass Foundation, administered by the MBL through J.J.R. It was also supported by Austrian Science fund FWF (P30686-B29) to H.S. and O.S., the Whitman Center Early Career Fellowship to O.S., the Okinawa Institute of Science and Technology Molecular Genetics Unit, Chan-Zuckerberg BioHub, and the Marthella Foskett Brown Chair in Computational Biology to D.S.R, NSF grant (IOS-1354898) to C.W.R, and the Hibbitt Early Career Fellowship to C.B.A. Sequencing at the University of Chicago Functional Genomics Facility was partially supported by the NIH (5UL1TR002389-02 and UL1 TR000430).
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  • 42
    Publication Date: 2022-08-19
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Yuan, D., Yin, X., Li, X., Corvianawatie, C., Wang, Z., Li, Y., Yang, Y., Hu, X., Wang, J., Tan, S., Surinati, D., Purwandana, A., Wardana, A., Ismail, M., Budiman, A., Bayhaqi, A., Avianto, P., Santoso, P., Kusmanto, E., Dirhamsyah, Arifin, Z., & Pratt, L. A Maluku Sea intermediate western boundary current connecting Pacific Ocean circulation to the Indonesian Throughflow. Nature Communications, 13(1), (2022): 2093, https://doi.org/10.1038/s41467-022-29617-6.
    Description: The Indonesian Throughflow plays an important role in the global ocean circulation and climate. Existing studies of the Indonesian Throughflow have focused on the Makassar Strait and the exit straits, where the upper thermocline currents carry North Pacific waters to the Indian Ocean. Here we show, using mooring observations, that a previous unknown intermediate western boundary current (with the core at ~1000 m depth) exists in the Maluku Sea, which transports intermediate waters (primarily the Antarctic Intermediate Water) from the Pacific into the Seram-Banda Seas through the Lifamatola Passage above the bottom overflow. Our results suggest the importance of the western boundary current in global ocean intermediate circulation and overturn. We anticipate that our study is the beginning of more extensive investigations of the intermediate circulation of the Indo-Pacific ocean in global overturn, which shall improve our understanding of ocean heat and CO2 storages significantly.
    Description: This study is supported by NSFC (D.Y., Z.W., Y.L., Y.Y., S.T., J.W., and X.L.: 41720104008; D.Y., J.W., Y.L., X.L., Y.Y., S.T., X.H., and X.Y.: 91858204), the National Key Research and Development Program of China (D.Y. and X.L.: 2020YFA0608800), CAS (D.Y., Z.W., J.W., and Y.L.: XDB42000000), projects. Affiliations 1 and 2 share the first position. D.Y. is supported by QMSNL (2018SDKJ0104-02), and Shandong Provincial (U1606402) and the “Kunpeng Outstanding Scholar Program” of the FIO/NMR of China, J.W. supported by NSFC (41776011), Z.W. by NSFC (41876025).
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  • 43
    Publication Date: 2022-06-29
    Description: Rapid and profound climatic and environmental changes have been predicted for the Antarctic Peninsula with so far unknown impact on the biogeochemistry of the continental shelves. In this study, we investigate benthic carbon sedimentation, remineralization and iron cycling using sediment cores retrieved on a 400 mile transect with contrasting sea ice conditions along the eastern shelf of the Antarctic Peninsula. Sediments at comparable water depths of 330-450 m showed sedimentation and remineralization rates of organic carbon, ranging from 2.5-13 and 1.8-7.2 mmol C m-2 d-1, respectively. Both rates were positively correlated with the occurrence of marginal sea ice conditions (5-35% ice cover) along the transect, suggesting a favorable influence of the corresponding light regime and water column stratification on algae growth and sedimentation rates. From south to north, the burial efficiency of organic carbon decreased from 58% to 27%, while bottom water temperatures increased from -1.9 to -0.1 °C. Net iron reduction rates, as estimated from pore-water profiles of dissolved iron, were significantly correlated with carbon degradation rates and contributed 0.7-1.2% to the total organic carbon remineralization. Tightly coupled phosphate-iron recycling was indicated by significant covariation of dissolved iron and phosphate concentrations, which almost consistently exhibited P/Fe flux ratios of 0.26. Iron efflux into bottom waters of 0.6-4.5 µmol Fe m-2 d-1 was estimated from an empirical model. Despite the deep shelf waters, a clear bentho-pelagic coupling is indicated, shaped by the extent and duration of marginal sea ice conditions during summer, and likely to be affected by future climate change.
    Repository Name: EPIC Alfred Wegener Institut
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  • 44
    Publication Date: 2022-06-29
    Description: The possibility that Arctic sea ice loss weakens mid-latitude westerlies, promoting more severe cold winters, has sparked more than a decade of scientific debate, with apparent support from observations but inconclusive modelling evidence. Here we show that sixteen models contributing to the Polar Amplification Model Intercomparison Project simulate a weakening of mid-latitude westerlies in response to projected Arctic sea ice loss. We develop an emergent constraint based on eddy feedback, which is 1.2 to 3 times too weak in the models, suggesting that the real-world weakening lies towards the higher end of the model simulations. Still, the modelled response to Arctic sea ice loss is weak: the North Atlantic Oscillation response is similar in magnitude and offsets the projected response to increased greenhouse gases, but would only account for around 10% of variations in individual years. We further find that relationships between Arctic sea ice and atmospheric circulation have weakened recently in observations and are no longer inconsistent with those in models.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , NonPeerReviewed , info:eu-repo/semantics/article
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  • 45
    Publication Date: 2022-06-24
    Description: A variety of tectonic processes spread along the circum-Mediterranean orogenic belts driven by the convergence of major plates, episodes of slab retreat and lateral and vertical mantle flows. Here, we provide an updated view of crustal stress and strain-rate fields for the Albanides belt in the eastern Adria-Eurasia convergence boundary. We framed a new geodetic-based source model for the 2019 Mw6.4 Durrёs earthquake in light of the regional deformation, propending for a transpressional west-dipping seismogenic fault. Our results highlight a fault-scale complexity which mirrors the long-time scale deformation of the Albanides plate boundary, where the rotation induced by the fast Hellenic rollback is accommodated also by transpression on inherited structures.
    Description: Published
    Description: 244–252
    Description: 3T. Fisica dei terremoti e Sorgente Sismica
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 46
    Publication Date: 2022-05-24
    Description: Pyroclastic currents are described as gravity currents, and the classic conceptual model gives a first-order importance to the density of such currents. This directs quantitative models to assume specific flow structures (shallow water or equilib rium turbulent boundary layer), which may apply to restricted volcanic areas inde pendently of source dynamics or may correspond to source dynamics separate from topographic interaction. The recent introduction of two end-members of pyroclastic currents, inertial and forced, is further developed here, leading to a global conceptual model in which source dynamics and topographic interaction are both taken into account. The concept of energy facies is defined here as the ensemble of the first order indicators of pyroclastic currents (topological aspect ratio, competence ratio and emplacement temperature) that are proxies of the energy of such currents. Nine energy facies are introduced with general applicability and with the goal to globally characterize pyroclastic currents from vent to deposit.
    Description: Published
    Description: 1-11
    Description: 4V. Processi pre-eruttivi
    Description: 5V. Processi eruttivi e post-eruttivi
    Description: JCR Journal
    Keywords: Energy facies ; pyroclastic currents
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 47
    Publication Date: 2022-09-15
    Description: Phytoplankton stand at the base of the marine food-web, and play a major role in global carbon cycling. Rising CO2 levels and temperatures are expected to enhance growth and alter carbon:nutrient stoichiometry of marine phytoplankton, with possible consequences for the functioning of marine food-webs and the oceanic carbon pump. To date, however, the consistency of phytoplankton stoichiometric responses remains unclear. We therefore performed a meta-analysis on data from experimental studies on stoichiometric responses of marine phytoplankton to elevated pCO2 and 3–5° warming under nutrient replete and limited conditions. Our results demonstrate that elevated pCO2 increased overall phytoplankton C:N (by 4%) and C:P (by 9%) molar ratios under nutrient replete conditions, as well as phytoplankton growth rates (by 6%). Nutrient limitation amplified the CO2 effect on C:N and C:P ratios, with increases to 27% and 17%, respectively. In contrast to elevated pCO2, warming did not consistently alter phytoplankton elemental composition. This could be attributed to species- and study-specific increases and decreases in stoichiometry in response to warming. While our observed moderate CO2-driven changes in stoichiometry are not likely to drive marked changes in food web functioning, they are in the same order of magnitude as current and projected estimations of oceanic carbon export. Therefore, our results may indicate a stoichiometric compensation mechanism for reduced oceanic carbon export due to declining primary production in the near future.
    Repository Name: EPIC Alfred Wegener Institut
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  • 48
    Publication Date: 2022-09-14
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Thomas, M., Jensen, F. H., Averly, B., Demartsev, V., Manser, M. B., Sainburg, T., Roch, M. A., & Strandburg-Peshkin, A. A practical guide for generating unsupervised, spectrogram-based latent space representations of animal vocalizations. The Journal of Animal Ecology, 91(8), (2022): 1567– 1581, https://doi.org/10.1111/1365-2656.13754.
    Description: 1. Background: The manual detection, analysis and classification of animal vocalizations in acoustic recordings is laborious and requires expert knowledge. Hence, there is a need for objective, generalizable methods that detect underlying patterns in these data, categorize sounds into distinct groups and quantify similarities between them. Among all computational methods that have been proposed to accomplish this, neighbourhood-based dimensionality reduction of spectrograms to produce a latent space representation of calls stands out for its conceptual simplicity and effectiveness. 2. Goal of the study/what was done: Using a dataset of manually annotated meerkat Suricata suricatta vocalizations, we demonstrate how this method can be used to obtain meaningful latent space representations that reflect the established taxonomy of call types. We analyse strengths and weaknesses of the proposed approach, give recommendations for its usage and show application examples, such as the classification of ambiguous calls and the detection of mislabelled calls. 3. What this means: All analyses are accompanied by example code to help researchers realize the potential of this method for the study of animal vocalizations.
    Description: This work was supported by HFSP Research Grant RGP0051/2019 to ASP, MBM and MAR, and funded by the Deutsche Forschungsgemeinschaft (DFG) under Germany's Excellence Strategy (EXC-2117-422037984). ASP received additional funding from the Gips-Schüle Stiftung, the Zukunftskolleg at the University of Konstanz and the Max-Planck-Institute of Animal Behaviour. VD was funded by the Minerva Stiftung and Alexander von Humboldt Foundation.
    Keywords: animal sounds ; animal vocalizations ; bioacoustics ; call classification ; dimensionality reduction ; spectrogram ; UMAP ; unsupervised learning
    Repository Name: Woods Hole Open Access Server
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  • 49
    Publication Date: 2022-09-23
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Pattanayak, R., Underwood, R., Crowley, M. R., Crossman, D. K., Morgan, J. R., & Yacoubian, T. A. Deletion in chromosome 6 spanning alpha-synuclein and multimerin1 loci in the Rab27a/b double knockout mouse. Scientific Reports, 12(1), (2022): 9837, https://doi.org/10.1038/s41598-022-13557-8.
    Description: We report an incidental 358.5 kb deletion spanning the region encoding for alpha-synuclein (αsyn) and multimerin1 (Mmrn1) in the Rab27a/Rab27b double knockout (DKO) mouse line previously developed by Tolmachova and colleagues in 2007. Western blot and RT-PCR studies revealed lack of αsyn expression at either the mRNA or protein level in Rab27a/b DKO mice. PCR of genomic DNA from Rab27a/b DKO mice demonstrated at least partial deletion of the Snca locus using primers targeted to exon 4 and exon 6. Most genes located in proximity to the Snca locus, including Atoh1, Atoh2, Gm5570, Gm4410, Gm43894, and Grid2, were shown not to be deleted by PCR except for Mmrn1. Using whole genomic sequencing, the complete deletion was mapped to chromosome 6 (60,678,870–61,037,354), a slightly smaller deletion region than that previously reported in the C57BL/6J substrain maintained by Envigo. Electron microscopy of cortex from these mice demonstrates abnormally enlarged synaptic terminals with reduced synaptic vesicle density, suggesting potential interplay between Rab27 isoforms and αsyn, which are all highly expressed at the synaptic terminal. Given this deletion involving several genes, the Rab27a/b DKO mouse line should be used with caution or with appropriate back-crossing to other C57BL/6J mouse substrain lines without this deletion.
    Description: This study was supported by NIH [R56NS115767 (TAY), RF1NS115767-01A1 (TAY), P50NS108675 (TAY), and NINDS/NIA RF1NS078165 (JRM)].
    Repository Name: Woods Hole Open Access Server
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  • 50
    Publication Date: 2022-07-20
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Ballou, L., Brankovits, D., Chavez-Solis, E. M., Diaz, J. M. C., Gonzalez, B. C., Rohret, S., Salinas, A., Liu, A., Simoes, N., Alvarez, F., Miglietta, M. P., Iliffe, T. M., & Borda, E. An integrative re-evaluation of Typhlatya shrimp within the karst aquifer of the Yucatan Peninsula, Mexico. Scientific Reports, 12(1), (2022): 5302, https://doi.org/10.1038/s41598-022-08779-9.
    Description: The Yucatán Peninsula, Mexico is a carbonate platform well-known for extensive karst networks of densely stratified aquifer ecosystems. This aquifer supports diverse anchialine fauna, including species of the globally distributed anchialine shrimp genus Typhlatya (Atyidae). Four species (T. campecheae, T. pearsei, T. dzilamensis and T. mitchelli) are endemic to the Peninsula, of which three are federally listed in Mexico. This first integrative evaluation (i.e., molecular, morphological, broad geographic and type locality sampling, and environmental data) of Yucatán Typhlatya reveals considerable species identity conflict in prior phylogenetic assessments, broad species ranges, syntopy within cave systems and five genetic lineages (of which two are new to science). Despite sampling from the type locality of endangered T. campecheae, specimens (and molecular data) were indistinguishable from vulnerable T. pearsei. Ancestral/divergence reconstructions support convergent evolution of a low-salinity ancestor for a post-Paleogene arc Yucatán + Cuba Typhlatya clade within the anchialine Atyidae clade. A secondary adaptation for the coastal-restricted euryhaline (2–37 psu), Typhlatya dzilamensis (unknown conservation status) was identified, while remaining species lineages were low-salinity (〈 5 psu) adapted and found within the meteoric lens of inland and coastal caves. This study demonstrates the need for integrative/interdisciplinary approaches when conducting biodiversity assessments in complex and poorly studied aquifers.
    Description: Financial support for this study was provided by Texas A&M-CONACYT (TI, FA), TI, FA), PAPIIT IN208519 (DGAPA-UNAM) (FA), CONACYT Ciencia Básica A1-S-32846 (FA), Texas A&M University San Antonio (TAMUSA) Start-up Funds (EB), TAMUSA Research Council Grant (EB), TAMUSA Summer Faculty Grant and Fellowship (EB). NSF-REU/OCE: 1560242 supported AL and EB, and TAMU-Louis Stokes Alliance for Minority Participation (HRD: 1612776) supported SR, LS. This work was also supported by the NSF Graduate Research Fellowship Program (M1703014) and the Cave Conservancy Foundation (LB). Typhlatya sampling was sanctioned under collection permits SEMARNAT/SGPA/DGVS 05263/14, 004471/18, 05996/19.
    Repository Name: Woods Hole Open Access Server
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  • 51
    Publication Date: 2022-07-21
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Kim, S., Park, J.-H., & Kug, J.-S. Tropical origins of the record-breaking 2020 summer rainfall extremes in East Asia. Scientific Reports, 12(1), (2022): 5366, https://doi.org/10.1038/s41598-022-09297-4.
    Description: The East Asian countries have experienced heavy rainfalls in boreal summer 2020. Here, we investigate the dynamical processes driving the rainfall extremes in East Asia during July and August. The Indian Ocean basin warming in June can be responsible for the anticyclonic anomalies in the western North Pacific (WNP), which modulate the zonally-elongated rainfalls in East Asia during July through an atmospheric Rossby wave train. In August, the East Asian rainfall increase is also related to the anticyclonic anomalies in the subtropical WNP, although it is located further north. The north tropical Atlantic warming in June partly contributes to the subtropical WNP rainfall decrease in August through a subtropical teleconnection. Then the subtropical WNP rainfall decrease drives the local anticyclonic anomalies that cause the rainfall increase in East Asia during August. The tropical Indian Ocean anomalously warmed in June and the subtropical WNP rainfall decreased in August 2020, which played a role in modulating the WNP anticyclonic anomalies. Therefore, the record-breaking rainfall extremes in East Asia that occurred during summer 2020 can be explained by the teleconnections associated with the tropical origins among the Indian, Pacific, and Atlantic Oceans and their interbasin interactions.
    Description: This work is supported by the National Research Foundation of Korea (NRF-2018R1A5A1024958 & NRF-2021M3I6A1086808).
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  • 52
    Publication Date: 2022-07-21
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Kellogg, R., Moosburner, M., Cohen, N., Hawco, N., McIlvin, M., Moran, D., DiTullio, G., Subhas, A., Allen, A., & Saito, M. Adaptive responses of marine diatoms to zinc scarcity and ecological implications. Nature Communications, 13(1), (2022): 1995, https://doi.org/10.1038/s41467-022-29603-y.
    Description: Scarce dissolved surface ocean concentrations of the essential algal micronutrient zinc suggest that Zn may influence the growth of phytoplankton such as diatoms, which are major contributors to marine primary productivity. However, the specific mechanisms by which diatoms acclimate to Zn deficiency are poorly understood. Using global proteomic analysis, we identified two proteins (ZCRP-A/B, Zn/Co Responsive Protein A/B) among four diatom species that became abundant under Zn/Co limitation. Characterization using reverse genetic techniques and homology data suggests putative Zn/Co chaperone and membrane-bound transport complex component roles for ZCRP-A (a COG0523 domain protein) and ZCRP-B, respectively. Metaproteomic detection of ZCRPs along a Pacific Ocean transect revealed increased abundances at the surface (〈200 m) where dZn and dCo were scarcest, implying Zn nutritional stress in marine algae is more prevalent than previously recognized. These results demonstrate multiple adaptive responses to Zn scarcity in marine diatoms that are deployed in low Zn regions of the Pacific Ocean.
    Description: This work was funded by the National Science Foundation (OCE-1736599 and OCE-1657766), NIH (R01GM135709), Gordon and Betty Moore Foundation (GBMF3782) to M.A.S., and Simons Foundation award 544236 to N.R.C. This work was further supported by the National Science Foundation (NSF-OCE-1756884 and NSF-MCB-1818390), United States Department of Energy (DE-SC0018344), and Gordon and Betty Moore Foundation grants GBMF3828 and GBMF5006 to A.E.A.
    Repository Name: Woods Hole Open Access Server
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  • 53
    Publication Date: 2022-07-20
    Description: The Antarctic krill (Euphausia superba Dana) is a keystone species in the Southern Ocean that uses an arsenal of hydrolases for biomacromolecule decomposition to effectively digest its omnivorous diet. The present study builds on a hybrid-assembled transcriptome (13,671 ORFs) combined with comprehensive proteome profiling. The analysis of individual krill compartments allowed detection of significantly more different proteins compared to that of the entire animal (1,464 vs. 294 proteins). The nearby krill sampling stations in the Bransfield Strait (Antarctic Peninsula) yielded rather uniform proteome datasets. Proteins related to energy production and lipid degradation were particularly abundant in the abdomen, agreeing with the high energy demand of muscle tissue. A total of 378 different biomacromolecule hydrolysing enzymes were detected, including 250 proteases, 99 CAZymes, 14 nucleases and 15 lipases. The large repertoire in proteases is in accord with the protein-rich diet affiliated with E. superba’s omnivorous lifestyle and complex biology. The richness in chitin-degrading enzymes allows not only digestion of zooplankton diet, but also the utilization of the discharged exoskeleton after moulting.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , NonPeerReviewed
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  • 54
    Publication Date: 2022-07-15
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Castellan, G., Angeletti, L., Montagna, P., & Taviani, M. Drawing the borders of the mesophotic zone of the Mediterranean Sea using satellite data. Scientific Reports, 12(1), (2022): 5585, https://doi.org/10.1038/s41598-022-09413-4.
    Description: The 30–150 m bathymetric range is commonly adopted in the literature to constrain the mesophotic zone. However, such depth interval varies depending on sunlight penetration, which is primarily a function of solar radiation incidence and water clarity. This is especially obvious in the Mediterranean Sea with its peculiar biophysical properties. Integrating information on light regime in the estimation of the bathymetric range of the mesophotic zone would provide a more robust definition, orienting conservation actions targeting its ecosystems. We present a first assessment of the spatial and vertical extension of the mesophotic zone in the Mediterranean Sea based upon light penetration, comparing our prediction with literature data. Our study also represents a baseline to monitor future variations in the bathymetric interval associated with the mesophotic zone in the Mediterranean Sea in relation to global changes.
    Description: This is ISMAR-CNR Bologna scientific contribution n. 2061. This work was supported by the “Convenzione MATTMCNR per i Programmi di Monitoraggio per la Direttiva sulla Strategia Marina (MSFD, Art. 11, Dir. 2008/56/CE)”, the H2020 Project RELIANCE (Grant agreement no: 101017501), the DG Environment programme IDEM (Grant agreement no. 11.0661/2017/750680/SUB/EN V.C2) and MIUR-PRIN 2017 GLIDE 2017FREXZY. This contribution is supported by Ph.D. program in Cultural and Natural Heritage of the University of Bologna (GC).
    Repository Name: Woods Hole Open Access Server
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  • 55
    Publication Date: 2022-07-15
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Millstein, J. D., Minchew, B. M., & Pegler, S. S. Ice viscosity is more sensitive to stress than commonly assumed. Communications Earth & Environment, 3(1), (2022): 57, https://doi.org/10.1038/s43247-022-00385-x.
    Description: Accurate representation of the viscous flow of ice is fundamental to understanding glacier dynamics and projecting sea-level rise. Ice viscosity is often described by a simple but largely untested and uncalibrated constitutive relation, Glen’s Flow Law, wherein the rate of deformation is proportional to stress raised to the power n. The value n = 3 is commonly prescribed in ice-flow models, though observations and experiments support a range of values across stresses and temperatures found on Earth. Here, we leverage recent remotely-sensed observations of Antarctic ice shelves to show that Glen’s Flow Law approximates the viscous flow of ice with n = 4.1 ± 0.4 in fast-flowing areas. The viscosity and flow rate of ice are therefore more sensitive to changes in stress than most ice-flow models allow. By calibrating the governing equation of ice deformation, our result is a pathway towards improving projections of future glacier change.
    Description: .D.M. was partially funded through an NSF Graduate Research Fellowship. J.D.M. and B.M.M. where partially funded through NSF-NERC award 1853918. B.M.M. received additional funding through NSF-NERC award 1739031.
    Repository Name: Woods Hole Open Access Server
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  • 56
    Publication Date: 2022-07-15
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Zhou, Y.-L., Mara, P., Cui, G.-J., Edgcomb, V., & Wang, Y. Microbiomes in the Challenger Deep slope and bottom-axis sediments. Nature Communications, 13(1), (2022): 1515, https://doi.org/10.1038/s41467-022-29144-4.
    Description: Hadal trenches are the deepest and most remote regions of the ocean. The 11-kilometer deep Challenger Deep is the least explored due to the technical challenges of sampling hadal depths. It receives organic matter and heavy metals from the overlying water column that accumulate differently across its V-shaped topography. Here, we collected sediments across the slope and bottom-axis of the Challenger Deep that enable insights into its in situ microbial communities. Analyses of 586 metagenome-assembled genomes retrieved from 37 metagenomes show distinct diversity and metabolic capacities between bottom-axis and slope sites. 26% of prokaryotic 16S rDNA reads in metagenomes were novel, with novelty increasing with water and sediment depths. These predominantly heterotrophic microbes can recycle macromolecules and utilize simple and complex hydrocarbons as carbon sources. Metagenome and metatranscriptome data support reduction and biotransformation of arsenate for energy gain in sediments that present a two-fold greater accumulation of arsenic compared to non-hadal sites. Complete pathways for anaerobic ammonia oxidation are predominantly identified in genomes recovered from bottom-axis sediments compared to slope sites. Our results expand knowledge of microbially-mediated elemental cycling in hadal sediments, and reveal differences in distribution of processes involved in nitrogen loss across the trench.
    Description: This study was supported by the Strategic Priority Research Program B of the Chinese Academy of Sciences (No. XDB06010201 to Y.W.).
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  • 57
    Publication Date: 2022-12-12
    Description: Author Posting. © The Author(s), 2022. This is the author's version of the work. It is posted here by permission of John Wiley & Sons for personal use, not for redistribution. The definitive version was published in . Journal of Phycology (2022), https://doi.org/10.1111/jpy.13230.
    Description: The marine green alga Brilliantia kiribatiensis gen. et sp. nov. is described from samples collected from the coral reefs of the Southern Line Islands, Republic of Kiribati, Pacific Ocean. Phylogenetic analysis of sequences of the large- and small-subunit rDNA and the rDNA internal transcribed spacer region revealed that Brilliantia is a member of the Boodleaceae (Cladophorales), containing the genera Apjohnia, Boodlea, Cladophoropsis, Chamaedoris, Phyllodictyon, and Struvea. Within this clade it formed a distinct lineage, sister to Struvea elegans, but more distantly related to the bona fide Struvea species (including the type S. plumosa). Brilliantia differs from the other genera by having a very simple architecture forming upright, unbranched, single-celled filaments attached to the substratum by a rhizoidal mat. Cell division occurs by segregative cell division only at the onset of reproduction. Based on current sample collection, B. kiribatiensis seems to be largely restricted to the Southern Line Islands, although it was also observed on neighboring islands, including Orona Atoll in the Phoenix Islands of Kiribati, and the Rangiroa and Takapoto Atolls in the Tuamotus of French Polynesia. This discovery highlights the likeliness that there is still much biodiversity yet to be discovered from these remote and pristine reefs of the central Pacific.
    Description: National Geographic Society
    Description: 2022-12-12
    Keywords: 18S nuclear ribosomal DNA ; Chlorophyta ; Cladophorales ; Molecular phylogeny ; Siphonocladales ; Ulvophyceae
    Repository Name: Woods Hole Open Access Server
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  • 58
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Diaz, B. P., Knowles, B., Johns, C. T., Laber, C. P., Bondoc, K. G. V., Haramaty, L., Natale, F., Harvey, E. L., Kramer, S. J., Bolaños, L. M., Lowenstein, D. P., Fredricks, H. F., Graff, J., Westberry, T. K., Mojica, K. D. A., Haëntjens, N., Baetge, N., Gaube, P., Boss, E., Carlson, C. A., Behrenfeld, M. J., Van Mooy, B. A. S., Bidle, K. D. Seasonal mixed layer depth shapes phytoplankton physiology, viral production, and accumulation in the North Atlantic. Nature Communications, 12(1), (2021): 6634, https://doi.org/10.1038/s41467-021-26836-1.
    Description: Seasonal shifts in phytoplankton accumulation and loss largely follow changes in mixed layer depth, but the impact of mixed layer depth on cell physiology remains unexplored. Here, we investigate the physiological state of phytoplankton populations associated with distinct bloom phases and mixing regimes in the North Atlantic. Stratification and deep mixing alter community physiology and viral production, effectively shaping accumulation rates. Communities in relatively deep, early-spring mixed layers are characterized by low levels of stress and high accumulation rates, while those in the recently shallowed mixed layers in late-spring have high levels of oxidative stress. Prolonged stratification into early autumn manifests in negative accumulation rates, along with pronounced signatures of compromised membranes, death-related protease activity, virus production, nutrient drawdown, and lipid markers indicative of nutrient stress. Positive accumulation renews during mixed layer deepening with transition into winter, concomitant with enhanced nutrient supply and lessened viral pressure.
    Description: This work was made possible by NASA’s Earth Science Program in support of the North Atlantic Aerosol and Marine Ecosystem Study (15-RRNES15-0011 and 0NSSC18K1563 to K.D.B.; NNX15AF30G to M.J.B.), as well as with support from the National Science Foundation (OIA-2021032 to K.D.B., OCE-157943 to C.A.C., and OCE-1756254 to B.A.S.V.M.), the Gordon and Betty Moore Foundation (Award# 3789 to K.G.V.B.), and NASA’s Future Investigators in Space Science and Technology program (FINESST; grant #826380 to K.D.B.; graduate support to BD).
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  • 59
    Publication Date: 2022-09-16
    Description: Marine protists abound, but are challenging to study, and their interactions with other microbes in nature remain largely unknown. We captured wild predatory protists (choanoflagellates) and discovered a divergent, obligately co-associated bacterial group that lives by extracting resources from these predators.
    Type: Article , NonPeerReviewed
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  • 60
    Publication Date: 2022-11-14
    Description: The Indo-Pacific Warm Pool (IPWP) exerts a dominant role in global climate by releasing huge amounts of water vapour and latent heat to the atmosphere and modulating upper ocean heat content (OHC), which has been implicated in modern climate change1. The long-term variations of IPWP OHC and their effect on monsoonal hydroclimate are, however, not fully explored. Here, by combining geochemical proxies and transient climate simulations, we show that changes of IPWP upper (0–200 m) OHC over the past 360,000 years exhibit dominant precession and weaker obliquity cycles and follow changes in meridional insolation gradients, and that only 30%–40% of the deglacial increases are related to changes in ice volume. On the precessional band, higher upper OHC correlates with oxygen isotope enrichments in IPWP surface water and concomitant depletion in East Asian precipitation as recorded in Chinese speleothems. Using an isotope-enabled air–sea coupled model, we suggest that on precessional timescales, variations in IPWP upper OHC, more than surface temperature, act to amplify the ocean–continent hydrological cycle via the convergence of moisture and latent heat. From an energetic viewpoint, the coupling of upper OHC and monsoon variations, both coordinated by insolation changes on orbital timescales, is critical for regulating the global hydroclimate.
    Type: Article , PeerReviewed
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  • 61
    Publication Date: 2022-12-19
    Description: New sedimentological data of facies and diagenesis as well as chronological data including strontium (87Sr/86Sr)-isotope ratios and uranium (U)-series dating, radiocarbon (14C) accelerator mass spectrometry (AMS) dating and biostratigraphy from elevated reef terraces (makatea) in the southern Cook Islands of Mangaia, Rarotonga and Aitutaki contribute to controversial discussions regarding age and sea-level relationships of these occurrences during the Neogene and Quaternary. The oldest limestones of the uplifted makatea island of Mangaia include reef-related facies which are mid-Miocene in age, based on new Sr-isotope and biostratigraphical data. In between these older deposits and the lowest coastal reef terrace of marine isotope stage (MIS) 5e, various older Pleistocene reef-related facies were identified. Based on Sr-isotope ratios, these were deposited during earlier Pleistocene highstands (as old as 2.28 Ma). Rare reef terraces on Rarotonga belong to the Plio-Pleistocene and the late Miocene, according to 87Sr/86Sr ratios. The late Miocene age is enigmatic as it exceeds the age of subaerially exposed volcanic rocks of Rarotonga island. The fossil reef could have formed on an older submarine volcanic high that was later displaced by younger volcanism to its present position, or the Sr-age could be too old due to diagenetic resetting. The Plio-Pleistocene Rarotonga reef terraces are overlain irregularly by Holocene reef deposits that are interpreted as storm rubble. Reef terraces on Aitutaki represent evidence of a higher-than-present (up to 1 m) sea-level during the late Holocene, based on 14C AMS age data. They are very similar to elevated late Holocene reefs of adjacent French Polynesia with regard to composition, elevation and age.
    Type: Book chapter , NonPeerReviewed
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  • 62
    Publication Date: 2022-03-07
    Description: Fjords are recognized as hotspots of organic carbon (OC) burial in the coastal ocean. In fjords with glaciated catchments, glacier discharge carries large amounts of suspended matter. This sedimentary load includes OC from bedrock and terrigenous sources (modern vegetation, peat, soil deposits), which is either buried in the fjord or remineralized during export, acting as a potential source of CO2 to the atmosphere. In sub-Antarctic South Georgia, fjord-terminating glaciers have been retreating during the past decades, likely as a response to changing climate conditions. We determine sources of OC in surface sediments of Cumberland Bay, South Georgia, using lipid biomarkers and the bulk 14C isotopic composition, and quantify OC burial at present and for the time period of documented glacier retreat (between 1958 and 2017). Petrogenic OC is the dominant type of OC in proximity to the present-day calving fronts (60.4 ± 1.4% to 73.8 ± 2.6%) and decreases to 14.0 ± 2.7% outside the fjord, indicating that petrogenic OC is effectively buried in the fjord. Beside of marine OC, terrigenous OC comprises 2.7 ± 0.5% to 7.9 ± 5.9% and is mostly derived from modern plants and Holocene peat and soil deposits that are eroded along the flanks of the fjord, rather than released by the retreating fjord glaciers. We estimate that the retreat of tidewater glaciers between 1958 and 2017 led to an increase in petrogenic carbon accumulation of 22% in Cumberland West Bay and 6.5% in Cumberland East Bay, suggesting that successive glacier retreat does not only release petrogenic OC into the fjord, but also increases the capacity of OC burial.
    Repository Name: EPIC Alfred Wegener Institut
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  • 63
    Publication Date: 2022-04-22
    Description: Integrated electron microprobe analyses (EMPAs) on glass and Sr–Nd isotope analyses have been performed on 17 tephras from the Middle Pleistocene Mercure lacustrine succession, southern Apennines. Two 40Ar/39Ar ages and the recognition of four relevant tephras from Colli Albani, Sabatini and possibly Roccamonfina volcanoes allowed us to ascribe the investigated succession to the late Marine Isotope Stage (MIS) 15–12 interval (560–440 ka). The Sr–Nd isotopes and major element glass compositions allowed us to attribute 10 out of the other 13 tephras to a poorly known activity of the Roccamofina volcano, whereas two layers were tentatively attributed to previously unknown Middle Pleistocene activity of Ponza Island or Campanian volcanoes, and one to Salina Island. The tephrostratigraphic correlation of the Mercure tephras with the Acerno lacustrine pollen record (Campania) also allowed us to evaluate the climatostratigraphic position of the tephras within the framework of the MIS 15–12 climatic variability. These results were obtained by combining the Sr–Nd isotope ratio with EMPA and 40Ar/39Ar geochronological data. This confirms the notable consistency of this approach for studying the Mediterranean Middle Pleistocene tephrostratigraphy, which, despite its great potential for both volcanology and Quaternary studies, has been hitherto barely explored.
    Description: Published
    Description: 232–248
    Description: 2V. Struttura e sistema di alimentazione dei vulcani
    Description: 3V. Proprietà chimico-fisiche dei magmi e dei prodotti vulcanici
    Description: 4V. Processi pre-eruttivi
    Description: 6A. Geochimica per l'ambiente e geologia medica
    Description: JCR Journal
    Keywords: 40Ar/39Ar dating; EMPA glass compositions ; Middle Pleistocene; ; peri-Tyrrhenian explosive volcanisms ; Sr isotopes.
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 64
    Publication Date: 2022-01-04
    Description: We present a novel method to estimate dynamic ice loss of Greenland's three largest outlet glaciers: Jakobshavn Isbræ, Kangerlussuaq Glacier, and Helheim Glacier. We use Global Navigation Satellite System (GNSS) stations attached to bedrock to measure elastic displacements of the solid Earth caused by dynamic thinning near the glacier terminus. When we compare our results with discharge, we find a time lag between glacier speedup/slowdown and onset of dynamic thinning/thickening. Our results show that dynamic thinning/thickening on Jakobshavn Isbræ occurs 0.87 ± 0.07 years before speedup/slowdown. This implies that using GNSS time series we are able to predict speedup/slowdown of Jakobshavn Isbræ by up to 10.4 months. For Kangerlussuaq Glacier the lag between thinning/thickening and speedup/slowdown is 0.37 ± 0.17 years (4.4 months). Our methodology and results could be important for studies that attempt to model and understand mechanisms controlling short-term dynamic fluctuations of outlet glaciers in Greenland.
    Repository Name: EPIC Alfred Wegener Institut
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  • 65
    Publication Date: 2021-11-29
    Description: This work presents a novel empirical Ground Motion prediction Model (GMM) for vertical-to-horizontal (VH) response spectral amplitudes up to 10 s, peak ground acceleration and velocity for shallow crustal earthquakes in Italy. Being calibrated on the most up-to-date strong motion dataset for Italian crustal earthquakes (ITA18), the model is consistent with the ITA18 GMM for the horizontal ground motion. This property makes the model useful in probabilistic seismic hazard assessment for Italy to derive compatible vertical and horizontal response spectra. To account for the increase of VH ratios in the proximity of the seismic source, an adjustment term is introduced to improve the prediction capability of the model in near-source conditions, relying on the worldwide NEar-Source Strong motion dataset (NESS). The proposed model uses a simple functional form restricted to a limited number of predictor variables, namely, magnitude, source-to-site distance, focal mechanism, and site effects, and the variability associated with both VH and V models is provided.
    Description: Published
    Description: 4121-4141
    Description: 5T. Sismologia, geofisica e geologia per l'ingegneria sismica
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 66
    Publication Date: 2021-12-16
    Description: Diffusive gradients in thin fi lms (DGT) have been tested in CO2-rich, metal-bearing fl uids from springs in the Campo de Calatrava region in Central Spain, to assess their applicability as a monitoring tool in onshore CO2 storage projects. These fi lms are capable of adsorbing metals and recording changes in their concentration in water, sediments, and soils. Considering that CO2 dissolution promotes metal solubilization and transport, the use of these fi lms could be valuable as a monitoring tool of early leakage. A number of DGT have been deployed in selected springs with constant metal concentration. The studied waters show high concentrations of Fe, as high as 1 × 104 μg·L–1, Ni, Co, Zn, Cu, and Mn. Comparing re-calculated metal concentration in DGT with metal water concentration, two different metal behaviors are observed: (i) metals with sorption consistent with the metal concentration (i.e. plotting close to the 1:1 line in a [Me]DGT: [Me]water plot), and (ii) metals with non–linear sorption, with some data showing metal enrichment in DGT compared with the concentration in water. Metals in the fi rst group include Fe, Mn, Co, Ni, and U, and metals in the second group are Zn, Pb, Cr, Cu, and Al. From this research, it is concluded that the metals in the fi rst group can be used to monitor potential leakage by using DGT, providing effective leakage detection even considering low variations of concentrations, episodic metal release, and reducing costs compared with conventional, periodic water sampling.
    Description: Published
    Description: 163-175
    Description: 6A. Geochimica per l'ambiente e geologia medica
    Description: JCR Journal
    Keywords: Campo de Calatrava ; CO2 storage and leakage ; DGT ; metal leakage ; metal transport ; trace metals ; 03.04. Chemical and biological ; 05.04. Instrumentation and techniques of general interest
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 67
    Publication Date: 2021-12-22
    Description: We present a new geometrical method capable of quantifying and illustrating the outcomes of a three-component mixing dynamics. In a three-component mixing sce nario, classical algebraic equations and endmember mixing analysis (EMMA) can be used to quantify the contributions from each fraction. Three-component mixing of natural waters, either in an element–element plot or by using the EMMA mixing sub space is described by a triangular shaped distribution of sample points where each endmember is placed on an apex, while each side corresponds to the mixing function of the two endmembers placed at the apex, considering the third endmembers' con tribution equal to zero. Along each side, the theoretical mixing fractions can be com puted using mass balance equations. Samples with contributions from three endmembers will plot inside the triangle, while the homogeneous barycentric coordi nate projections can be projected onto the three sides. The geochemistry observed in the mineralized Ferrarelle aquifer system (southern Italy) results from three component mixing of groundwater, each with diagnostic geochemical compositions. The defined boundary conditions allow us to parameterize and validate the proce dures for modelling mixing, including selection of suitable geochemical tracer
    Description: Published
    Description: e14409
    Description: 6A. Geochimica per l'ambiente e geologia medica
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 68
    Publication Date: 2022-05-24
    Description: We have reinterpreted the causative fault parameters of the 2005 Zarand earthquake in the light of a new imagery study using Interferometric Synthetic Aperture Radar (InSAR). By conducting a joint inversion of two InSAR datasets, we can characterize the rupture as it relates to complex local structures. At first, the mainshock ruptured a nearly pure reverse fault, dipping ~65° NNW in the basement below the southeastern area of Zarand. Two more fault segments were subsequently activated: an oblique‐normal fault segment parallel to the first segment, dipping 61° to the south, and a normal‐oblique fault segment at the eastern termination of the rupture zone. The first fault segment ruptured the surface, while slip along the other two segments was confined to the lower sedimentary strata.
    Description: Published
    Description: 274-283
    Description: 2T. Deformazione crostale attiva
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 69
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Boissin, E., Neglia, V., Baksay, S., Micu, D., Bat, L., Topaloglu, B., Todorova, V., Panayotova, M., Kruschel, C., Milchakova, N., Voutsinas, E., Beqiraj, S., Nasto, I., Aglieri, G., Taviani, M., Zane, L., & Planes, S. Chaotic genetic structure and past demographic expansion of the invasive gastropod Tritia neritea in its native range, the Mediterranean Sea. Scientific Reports, 10(1), (2020): 21624. doi:10.1038/s41598-020-77742-3.
    Description: To better predict population evolution of invasive species in introduced areas it is critical to identify and understand the mechanisms driving genetic diversity and structure in their native range. Here, we combined analyses of the mitochondrial COI gene and 11 microsatellite markers to investigate both past demographic history and contemporaneous genetic structure in the native area of the gastropod Tritia neritea, using Bayesian skyline plots (BSP), multivariate analyses and Bayesian clustering. The BSP framework revealed population expansions, dated after the last glacial maximum. The haplotype network revealed a strong geographic clustering. Multivariate analyses and Bayesian clustering highlighted the strong genetic structure at all scales, between the Black Sea and the Adriatic Sea, but also within basins. Within basins, a random pattern of genetic patchiness was observed, suggesting a superimposition of processes involving natural biological effects (no larval phase and thus limited larval dispersal) and putative anthropogenic transport of specimens. Contrary to the introduced area, no isolation-by-distance patterns were recovered in the Mediterranean or the Black Seas, highlighting different mechanisms at play on both native and introduced areas, triggering unknown consequences for species’ evolutionary trajectories. These results of Tritia neritea populations on its native range highlight a mixture of ancient and recent processes, with the effects of paleoclimates and life history traits likely tangled with the effects of human-mediated dispersal.
    Description: This project was funded by the European FP7 CoCoNet project (Ocean.2011-4, grant agreement #287844) and we are grateful to the whole CoCoNET consortium. We are grateful to the following people for their critical help with logistics and field work ‘Antheus srl (Lecce, Italy)’; S Bevilacqua, G Guarnieri, S Fraschetti and T Terlizzi (University of Salento, Italy); L Angeletti and M Sigovini (ISMAR, Italy); D Shamrey (IBSS, Sevastopol); A Anastasopoulou, MA Pancucci-Papadopoulou and S Reizopoulou (HCMR, Greece) and E Hajdëri (Catholic University ‘Our Lady of Good Counsel’, Tirana). Thank you to J Almany for English corrections. This is ISMAR-CNR scientific contribution n1987. E Boissin was supported by a European Marie Curie postdoctoral fellowship MC-CIG-618480.
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  • 70
    Publication Date: 2022-05-26
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Mayfield, K. K., Eisenhauer, A., Santiago Ramos, D. P., Higgins, J. A., Horner, T. J., Auro, M., Magna, T., Moosdorf, N., Charette, M. A., Gonneea, M. E., Brady, C. E., Komar, N., Peucker-Ehrenbrink, B., & Paytan, A. Groundwater discharge impacts marine isotope budgets of Li, Mg, Ca, Sr, and Ba. Nature Communications, 12(1), (2021): 148-020-20248-3, doi:10.1038/s41467-020-20248-3.
    Description: Groundwater-derived solute fluxes to the ocean have long been assumed static and subordinate to riverine fluxes, if not neglected entirely, in marine isotope budgets. Here we present concentration and isotope data for Li, Mg, Ca, Sr, and Ba in coastal groundwaters to constrain the importance of groundwater discharge in mediating the magnitude and isotopic composition of terrestrially derived solute fluxes to the ocean. Data were extrapolated globally using three independent volumetric estimates of groundwater discharge to coastal waters, from which we estimate that groundwater-derived solute fluxes represent, at a minimum, 5% of riverine fluxes for Li, Mg, Ca, Sr, and Ba. The isotopic compositions of the groundwater-derived Mg, Ca, and Sr fluxes are distinct from global riverine averages, while Li and Ba fluxes are isotopically indistinguishable from rivers. These differences reflect a strong dependence on coastal lithology that should be considered a priority for parameterization in Earth-system models.
    Description: We thank A. Beck, H. Dulai, I. Santos, C. Benitez-Nelson, W. Moore, A. Martin, and H. Windom for sample access. We also thank A. Kolevica, A. Heuser, H. Pryer, J. Middleton, R. Franks, F. Lon, N. Slater, and O. Šebek for their laboratory and analytical assistance. This material is based upon research supported by the National Science Foundation Graduate Research Fellowship Program and an internship provided through the U.S. Geological Survey Graduate Research Internship Program (GRIP). This research was also supported by grants from: the German Academic Exchange Service (DAAD), Northern California chapter of the Achievement Rewards for College Scientists Foundation, International Association of GeoChemistry, Geological Society of America, Northern California Geological Society, Myers Trust, Friends of Long Marine Lab, and UC MEXUS (to K.K.M.). We acknowledge funding from EU-ITN Horizon project 643084 (to A.E. and T.M.) and NSF grant Award Number 1259440 (to A.P.). We also acknowledge funding from NSF grant award number OCE-1736949 (to T.J.H.). Any use of trade, firm or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.
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  • 71
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Skottene, E., Tarrant, A. M., Altin, D., Olsen, R. E., Choquet, M., & Kvile, K. O. Lipid metabolism in Calanus finmarchicus is sensitive to variations in predation risk and food availability. Scientific Reports, 10(1),(2020): 22322, https://doi.org/10.1038/s41598-020-79165-6.
    Description: Late developmental stages of the marine copepods in the genus Calanus can spend extended periods in a dormant stage (diapause) that is preceded by the accumulation of large lipid stores. We assessed how lipid metabolism during development from the C4 stage to adult is altered in response to predation risk and varying food availability, to ultimately understand more of the metabolic processes during development in Calanus copepods. We used RNA sequencing to assess if perceived predation risk in combination with varied food availability affects expression of genes associated with lipid metabolism and diapause preparation in C. finmarchicus. The lipid metabolism response to predation risk differed depending on food availability, time and life stage. Predation risk caused upregulation of lipid catabolism with high food, and downregulation with low food. Under low food conditions, predation risk disrupted lipid accumulation. The copepods showed no clear signs of diapause preparation, supporting earlier observations of the importance of multiple environmental cues in inducing diapause in C. finmarchicus. This study demonstrates that lipid metabolism is a sensitive endpoint for the interacting environmental effects of predation pressure and food availability. As diapause may be controlled by lipid accumulation, our findings may contribute towards understanding processes that can ultimately influence diapause timing.
    Description: ES was funded by the Department of Biology, Norwegian University of Science and Technology. Support for AMT was provided by the National Science Foundation (NSF) OPP-1746087. KØK was funded by VISTA—a basic research program in collaboration between The Norwegian Academy of Science and Letters, and Equinor. The RNA seq work was provided by the Genomics Core Facility (GCF). GCF is funded by the Faculty of Medicine and Health Sciences at NTNU and Central Norway Regional Health Authority. The authors thank Lotte Thommesen for help conducting the experiment, Mari-Ann Østensen for performing RNA extraction with ES and preparing cDNA libraries for Illumina RNAseq, and the EMBRC-ERIC Laboratory for Low Level Trophic Interactions at NTNU SeaLab for access to the Calanus finmarchicus culture.
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  • 72
    Publication Date: 2022-10-26
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Jin, D., Hoagland, P., & Ashton, A. D. Risk averse choices of managed beach widths under environmental uncertainty. Natural Resource Modeling, (2021): e12324, https://doi.org/10.1111/nrm.12324.
    Description: Applying a theoretical geo-economic approach, we examined key factors affecting decisions about the choice of beach width when eroded coastal beaches are being nourished (i.e., when fill is placed to widen a beach). Within this geo-economic framework, optimal beach width is positively related to its values for hazard protection and recreation and negatively related to nourishment costs and the discount rate. Using a dynamic modeling framework, we investigated the time paths of beach width and nourishment that maximized net present value under an accelerating sea level. We then analyzed how environmental uncertainty about expected future beach width, arising from natural shoreline dynamics, intermittent large storms, or sea-level rise, leads to economic choices favoring narrower beaches. Risk aversion can affect a coastal property owner's choice of beach width in contradictory ways: the expected benefits of hazard protection must be balanced against the expected costs of repeated nourishment actions.
    Description: Support for this study was provided by NSF Grant No. ARG 1518503, WHOI Sea Grant (NOAA Award Number: NA18OAR4170104), and the J. Seward Johnson Fund in Support of the Marine Policy Center.
    Keywords: Beach nourishment ; Beach width ; Coastal protection ; Risk management ; Shoreline change
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  • 73
    Publication Date: 2022-10-26
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Farrell, U. C., Samawi, R., Anjanappa, S., Klykov, R., Adeboye, O. O., Agic, H., Ahm, A.-S. C., Boag, T. H., Bowyer, F., Brocks, J. J., Brunoir, T. N., Canfield, D. E., Chen, X., Cheng, M., Clarkson, M. O., Cole, D. B., Cordie, D. R., Crockford, P. W., Cui, H., Dahl, T. W., Mouro, L. D., Dewing, K., Dornbos, S. Q., Drabon, N., Dumoulin, J. A., Emmings, J. F., Endriga, C. R., Fraser, T. A., Gaines, R. R., Gaschnig, R. M., Gibson, T. M., Gilleaudeau, G. J., Gill, B. C., Goldberg, K., Guilbaud, R., Halverson, G. P., Hammarlund, E. U., Hantsoo, K. G., Henderson, M. A., Hodgskiss, M. S. W., Horner, Tristan J., Husson, J. M., Johnson, B., Kabanov, P., Brenhin K. C., Kimmig, J., Kipp, M. A., Knoll, A. H., Kreitsmann, T., Kunzmann, M., Kurzweil, F., LeRoy, M. A., Li, C., Lipp, A. G., Loydell, D. K., Lu, X., Macdonald, F. A., Magnall, J. M., Mänd, K., Mehra, A., Melchin, M. J., Miller, A. J., Mills, N. T., Mwinde, C. N., O'Connell, B., Och, L. M., Ossa Ossa, F., Pagès, A., Paiste, K., Partin, C. A., Peters, S. E., Petrov, P., Playter, T. L., Plaza-Torres, S., Porter, Susannah M., Poulton, S. W., Pruss, S. B., Richoz, S., Ritzer, S. R., Rooney, A. D., Sahoo, S. K., Schoepfer, S. D., Sclafani, J. A., Shen, Y., Shorttle, O., Slotznick, S. P., Smith, E. F., Spinks, S., Stockey, R. G., Strauss, J. V., Stüeken, E. E., Tecklenburg, S., Thomson, D., Tosca, N. J., Uhlein, G. J., Vizcaíno, M. N., Wang, H., White, T., Wilby, P. R., Woltz, C. R., Wood, R. A., Xiang, L., Yurchenko, I. A., Zhang, T., Planavsky, N. J., Lau, K. V., Johnston, D. T., Sperling, E. A., The Sedimentary Geochemistry and Paleoenvironments Project. Geobiology. 00, (2021): 1– 12,https://doi.org/10.1111/gbi.12462.
    Description: Geobiology explores how Earth's system has changed over the course of geologic history and how living organisms on this planet are impacted by or are indeed causing these changes. For decades, geologists, paleontologists, and geochemists have generated data to investigate these topics. Foundational efforts in sedimentary geochemistry utilized spreadsheets for data storage and analysis, suitable for several thousand samples, but not practical or scalable for larger, more complex datasets. As results have accumulated, researchers have increasingly gravitated toward larger compilations and statistical tools. New data frameworks have become necessary to handle larger sample sets and encourage more sophisticated or even standardized statistical analyses. In this paper, we describe the Sedimentary Geochemistry and Paleoenvironments Project (SGP; Figure 1), which is an open, community-oriented, database-driven research consortium. The goals of SGP are to (1) create a relational database tailored to the needs of the deep-time (millions to billions of years) sedimentary geochemical research community, including assembling and curating published and associated unpublished data; (2) create a website where data can be retrieved in a flexible way; and (3) build a collaborative consortium where researchers are incentivized to contribute data by giving them priority access and the opportunity to work on exciting questions in group papers. Finally, and more idealistically, the goal was to establish a culture of modern data management and data analysis in sedimentary geochemistry. Relative to many other fields, the main emphasis in our field has been on instrument measurement of sedimentary geochemical data rather than data analysis (compared with fields like ecology, for instance, where the post-experiment ANOVA (analysis of variance) is customary). Thus, the longer-term goal was to build a collaborative environment where geobiologists and geologists can work and learn together to assess changes in geochemical signatures through Earth history.
    Description: We thank the donors of The American Chemical Society Petroleum Research Fund for partial support of SGP website development (61017-ND2). EAS is funded by National Science Foundation grant (NSF) EAR-1922966. BGS authors (JE, PW) publish with permission of the Executive Director of the British Geological Survey, UKRI.
    Keywords: Consortium ; Database ; Earth history ; Geochemistry ; Website
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  • 74
    Publication Date: 2022-10-20
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Umanzor, S., Li, Y., Bailey, D., Augyte, S., Huang, M., Marty-Rivera, M., Jannink, J., Yarish, C., & Lindell, S. Comparative analysis of morphometric traits of farmed sugar kelp and skinny kelp, Saccharina spp., strains from the Northwest Atlantic. Journal of the World Aquaculture Society, (2021), https://doi.org/10.1111/jwas.12783.
    Description: Our team has initiated a selective breeding program for regional strains of sugar kelp, Saccharina latissima, to improve the competitiveness of kelp farming in the United States. Within our breeding program, we also include an endemic putative species, Saccharina angustissima, locally referred to as skinny kelp. We crossed uniclonal gametophyte cultures derived from 37 wild‐collected blades representing five sugar kelp strains and one skinny kelp strain to produce 104 unique crosses. Each cross was outplanted on a near‐shore research farm located in the Gulf of Maine (GOM). After the first farming season, our results indicated that sugar kelp and skinny kelp were interfertile, and produced mature and reproductively viable sporophytes. Morphological traits of individual blades varied depending on the parental contribution (sugar vs. skinny), with significant differences found in progeny blade length, width, thickness, and in stipe length and diameter. Despite these differences, wet weight and blade density per plot showed no statistical differences regardless of the cross. Given their published genetic similarity and their interfertility shown here, S. angustissima and S. latissima may not be different species, and may each contribute genetic diversity to breeding programs aimed at meeting ocean farming and market needs.
    Description: Funding was provided by the U.S. Department of Energy, ARPAe MARINER project contract number DE‐AR0000915 and DE‐AR0000911, AgCore Technologies of Rhode Island, and the Massachusetts Clean Energy Center, AmplifyMass Program.
    Keywords: Morphometrics ; Phenotyping ; Saccharina angustissima ; Saccharina latissima ; Seaweed aquaculture ; Selective breeding
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  • 75
    Publication Date: 2022-10-20
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Caruso, F., Hickmott, L., Warren, J. D., Segre, P., Chiang, G., Bahamonde, P., Español-Jiménez, S., Li, S., & Bocconcelli, A. Diel differences in blue whale (Balaenoptera musculus) dive behavior increase nighttime risk of ship strikes in northern Chilean Patagonia. Integrative Zoology, (2020): 1-18, doi:10.1111/1749-4877.12501.
    Description: The northern Chilean Patagonia region is a key feeding ground and a nursing habitat in the southern hemisphere for blue whales (Balaenoptera musculus). From 2014 to 2019, during 6 separate research cruises, the dive behavior of 28 individual blue whales was investigated using bio‐logging tags (DTAGs), generating ≈190 h of data. Whales dove to significantly greater depths during the day compared to nighttime (day: 32.6 ± 18.7 m; night: 6.2 ± 2.7 m; P 〈 0.01). During the night, most time was spent close to the surface (86% ± 9.4%; P 〈 0.01) and at depths of less than 12 m. From 2016 to 2019, active acoustics (scientific echosounders) were used to record prey (euphausiids) density and distribution simultaneously with whale diving data. Tagged whales appeared to perform dives relative to the vertical migration of prey during the day. The association between diurnal prey migration and shallow nighttime dive behavior suggests that blue whales are at increased risk of ship collisions during periods of darkness since the estimated maximum ship draft of vessels operating in the region is also ≈12 m. In recent decades, northern Chilean Patagonia has seen a large increase in marine traffic due to a boom in salmon aquaculture and the passenger ship industry. Vessel strike risks for large whales are likely underestimated in this region. Results reported in this study may be valuable for policy and mitigation decisions regarding conservation of the endangered blue whale.
    Description: This work was conducted under Chilean research permit PINV 38–2014 Ballena Azul, Golfo Corcovado, from the Ministerio de Economia, Fomento y Turismo, Subsecreteria de Pesca y Acuicultura. We would like to thank the crews of the vessels Centinela, Khronos and Solidaridad for their involvement in the fieldwork. Special thanks to Rafaela Landea‐Briones, Gloria Howes, Esteban Tapia Brunet, Pepe Montt, Thomas Montt, and Daniel Casado for helping and welcoming us in Patagonia. Thanks to MERI Foundation and their students Carlos Cantergiani, Andrea Hirmas and Elvira Vergara for their support and contributions to field efforts. We extend our gratitude to our collaborators Laela Sayigh, Michael Moore, Daniel Zitterbart, Frants Jensen, Aran Mooney, John Durban, Jeremy Goldbogen, and Dave Cade. Thanks to WHOI for financial and technical support. The data analysis and paper writing was financially supported by the National Key Research and Development Program of China (Grant number 2016YFC0300802); the biodiversity investigation, observation and assessment program (2019‐2023) of the Ministry of Ecology and Environment of China; and Indian Ocean Ninety‐east Ridge Ecosystem and Marine Environment Monitoring and Protection, supported by the China Ocean Mineral Resources R&D Association (no. DY135‐E2‐4). Additionally, FC thanks the President's International Fellowship Initiative (PIFI) of the Chinese Academy of Sciences.
    Keywords: Bio‐logging tags ; Blue whale ; Diving profile ; Ocean conservation ; Prey distribution
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  • 76
    Publication Date: 2022-10-26
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Patrick, S. C., Martin, J. G. A., Ummenhofer, C. C., Corbeau, A., & Weimerskirch, H. Albatrosses respond adaptively to climate variability by changing variance in a foraging trait. Global Change Biology, (2021), https://doi.org/10.1111/gcb.15735.
    Description: The ability of individuals and populations to adapt to a changing climate is a key determinant of population dynamics. While changes in mean behaviour are well studied, changes in trait variance have been largely ignored, despite being assumed to be crucial for adapting to a changing environment. As the ability to acquire resources is essential to both reproduction and survival, changes in behaviours that maximize resource acquisition should be under selection. Here, using foraging trip duration data collected over 7 years on black-browed albatrosses (Thalassarche melanophris) on the Kerguelen Islands in the southern Indian Ocean, we examined the importance of changes in the mean and variance in foraging behaviour, and the associated effects on fitness, in response to the El Niño Southern Oscillation (ENSO). Using double hierarchical models, we found no evidence that individuals change their mean foraging trip duration in response to a changing environment, but found strong evidence of changes in variance. Younger birds showed greater variability in foraging trip duration in poor conditions as did birds with higher fitness. However, during brooding, birds showed greater variability in foraging behaviour under good conditions, suggesting that optimal conditions allow the alteration between chick provisioning and self-maintenance trips. We found weak correlations between sea surface temperature and the ENSO, but stronger links with sea-level pressure. We suggest that variability in behavioural traits affecting resource acquisition is under selection and offers a mechanism by which individuals can adapt to a changing climate. Studies which look only at effects on mean behaviour may underestimate the effects of climate change and fail to consider variance in traits as a key evolutionary force.
    Description: The authors thank the Institut Polaire Français Paul Emile Victor (IPEV, programme 109 to HW) for providing financial and logistical support for the field work at Kerguelen, and to the Terres Australes et Antarctique Francaises (TAAF). The usage of the following data sets is gratefully acknowledged: SOI, NCEP/NCAR SLP and NOAA OISST v2, all provided by NOAA/OAR/ESRL PSD, Boulder, Colorado, USA, through https://www.esrl.noaa.gov/psd. CCU acknowledges support from the Joint Initiative Awards Fund from the Andrew W. Mellon Foundation and the James E. and Barbara V. Moltz Fellowship for Climate-Related Research.
    Keywords: Bet-hedging ; Intra-individual variability ; Resource acquisition ; Salt-water immersion logger ; Seabirds ; Southern Oscillation Index
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  • 77
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Nielsen, S. G., Bekaert, D. V., & Auro, M. Isotopic evidence for the formation of the moon in a canonical giant impact. Nature Communications, 12(1), (2021): 1817, https://doi.org/10.1038/s41467-021-22155-7.
    Description: Isotopic measurements of lunar and terrestrial rocks have revealed that, unlike any other body in the solar system, the Moon is indistinguishable from the Earth for nearly every isotopic system. This observation, however, contradicts predictions by the standard model for the origin of the Moon, the canonical giant impact. Here we show that the vanadium isotopic composition of the Moon is offset from that of the bulk silicate Earth by 0.18 ± 0.04 parts per thousand towards the chondritic value. This offset most likely results from isotope fractionation on proto-Earth during the main stage of terrestrial core formation (pre-giant impact), followed by a canonical giant impact where ~80% of the Moon originates from the impactor of chondritic composition. Our data refute the possibility of post-giant impact equilibration between the Earth and Moon, and implies that the impactor and proto-Earth mainly accreted from a common isotopic reservoir in the inner solar system.
    Description: This study was funded by NASA Emerging Worlds grant NNX16AD36G to S.G.N. We thank NASA-JSC, Tony Irving, and Thorsten Kleine for access to meteorite and Apollo mission samples. US Antarctic meteorite samples are recovered by the Antarctic Search for Meteorites (ANSMET) program, which has been funded by NSF and NASA, and characterized and curated by the Astromaterials Curation Office at NASA Johnson Space Center and the Department of Mineral Sciences of the Smithsonian Institution. J. Blusztajn is thanked for help with mass spectrometry support at WHOI.
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  • 78
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Wei, C., Hoffmann-Kuhnt, M., Au, W. W. L., Ho, A. Z. H., Matrai, E., Feng, W., Ketten, D. R., & Zhang, Y. Possible limitations of dolphin echolocation: a simulation study based on a cross-modal matching experiment. Scientific Reports, 11(1), (2021): 6689, https://doi.org/10.1038/s41598-021-85063-2.
    Description: Dolphins use their biosonar to discriminate objects with different features through the returning echoes. Cross-modal matching experiments were conducted with a resident bottlenose dolphin (Tursiops aduncus). Four types of objects composed of different materials (water-filled PVC pipes, air-filled PVC pipes, foam ball arrays, and PVC pipes wrapped in closed-cell foam) were used in the experiments, respectively. The size and position of the objects remained the same in each case. The data collected in the experiment showed that the dolphin’s matching accuracy was significantly different across the cases. To gain insight into the underlying mechanism in the experiments, we used finite element methods to construct two-dimensional target detection models of an echolocating dolphin in the vertical plane, based on computed tomography scan data. The acoustic processes of the click’s interaction with the objects and the surrounding media in the four cases were simulated and compared. The simulation results provide some possible explanations for why the dolphin performed differently when discriminating the objects that only differed in material composition in the previous matching experiments.
    Description: One of the authors, Wei. C is supported by a Forrest Research Foundation Fellowship. Support for D. Ketten for this effort was provided by the Joint Industry Programme and by the Helmholtz Foundation. This work was also supported by the Hawaii Institute of Marine Biology (HIMB) contribution No. 1630 and School of Ocean and Earth Science and Technology (SOEST) contribution No. 9452.
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  • 79
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Tani, T., Koike-Tani, M., Tran, M. T., Shribak, M., & Levic, S. Postnatal structural development of mammalian basilar membrane provides anatomical basis for the maturation of tonotopic maps and frequency tuning. Scientific Reports, 11(1), (2021): 7581, https://doi.org/10.1038/s41598-021-87150-w.
    Description: The basilar membrane (BM) of the mammalian cochlea constitutes a spiraling acellular ribbon that is intimately attached to the organ of Corti. Its graded stiffness, increasing from apex to the base of the cochlea provides the mechanical basis for sound frequency analysis. Despite its central role in auditory signal transduction, virtually nothing is known about the BM’s structural development. Using polarized light microscopy, the present study characterized the architectural transformations of freshly dissected BM at time points during postnatal development and maturation. The results indicate that the BM structural elements increase progressively in size, becoming radially aligned and more tightly packed with maturation and reach the adult structural signature by postnatal day 20 (P20). The findings provide insight into structural details and developmental changes of the mammalian BM, suggesting that BM is a dynamic structure that changes throughout the life of an animal.
    Description: This work was supported by Medical Research Council grant MR/ N004299/1 (to SL), University of Sussex Research Innovation Funds (to SL), Brighton and Sussex Medical School research funds (to SL), National Institute of General Medical Sciences, NIH grant R01 GM101701 (to MS), NIH R01 GM100160 (to TT) and KAKENHI JP18K19962 (to TT).
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  • 80
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in MacKinnon, J. A., Simmons, H. L., Hargrove, J., Thomson, J., Peacock, T., Alford, M. H., Barton, B., Boury, S., Brenner, S. D., Couto, N., Danielson, S. L., Fine, E. C., Graber, H. C., Guthrie, J., Hopkins, J. E., Jayne, S. R., Jeon, C., Klenz, T., Lee, C. M., Lenn, Y-D., Lucas, A. J., Lund, B., Mahaffey, C., Norman, L., Rainville, L., Smith, M. M., Thomas, L. N., Torres-Valdés, S., & Wood, K. R. A warm jet in a cold ocean. Nature Communications, 12(1), (2021): 2418, https://doi.org/10.1038/s41467-021-22505-5.
    Description: Unprecedented quantities of heat are entering the Pacific sector of the Arctic Ocean through Bering Strait, particularly during summer months. Though some heat is lost to the atmosphere during autumn cooling, a significant fraction of the incoming warm, salty water subducts (dives beneath) below a cooler fresher layer of near-surface water, subsequently extending hundreds of kilometers into the Beaufort Gyre. Upward turbulent mixing of these sub-surface pockets of heat is likely accelerating sea ice melt in the region. This Pacific-origin water brings both heat and unique biogeochemical properties, contributing to a changing Arctic ecosystem. However, our ability to understand or forecast the role of this incoming water mass has been hampered by lack of understanding of the physical processes controlling subduction and evolution of this this warm water. Crucially, the processes seen here occur at small horizontal scales not resolved by regional forecast models or climate simulations; new parameterizations must be developed that accurately represent the physics. Here we present novel high resolution observations showing the detailed process of subduction and initial evolution of warm Pacific-origin water in the southern Beaufort Gyre.
    Description: Support for this work was provided by the US Office of Naval Research Stratified Ocean Dynamics of the Arctic program (grant numbers N000141512903, N000141612378, N000141612377, N000141612379, N0001416123450, N000141612360, N000141612349, N000141812007, N000141812475, and N000141912514). Additional support for biogeochemistry sampling was provided by UK (NERC) and Germany (BMBF) through the Changing Arctic Ocean Program’s ARISE (NE/P006035/1, NE/P006000/2), PEANUTS (NE/R01275X/1, NE/R012547/2, and BMBF 03F0804) projects and the UK-France PhD program DGA/Dstl. Float deployments and hydrographic data compilations were supported in part by North Pacific Research Board grants A91-99a and A91-00a. The deployment of autonomous ocean profilers was supported by ONR, NOAA Research, and the Joint Institute for the Study of the Atmosphere and Ocean (JISAO) under NOAA Cooperative Agreement NA15OAR4320063.
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  • 81
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Sivaguru, M., Todorov, L. G., Fouke, C. E., Munro, C. M. O., Fouke, K. W., Fouke, K. E., Baughman, M. E., & Fouke, B. W. Corals regulate the distribution and abundance of Symbiodiniaceae and biomolecules in response to changing water depth and sea surface temperature. Scientific Reports, 11(1), (2021): 2230, https://doi.org/10.1038/s41598-021-81520-0.
    Description: The Scleractinian corals Orbicella annularis and O. faveolata have survived by acclimatizing to environmental changes in water depth and sea surface temperature (SST). However, the complex physiological mechanisms by which this is achieved remain only partially understood, limiting the accurate prediction of coral response to future climate change. This study quantitatively tracks spatial and temporal changes in Symbiodiniaceae and biomolecule (chromatophores, calmodulin, carbonic anhydrase and mucus) abundance that are essential to the processes of acclimatization and biomineralization. Decalcified tissues from intact healthy Orbicella biopsies, collected across water depths and seasonal SST changes on Curaçao, were analyzed with novel autofluorescence and immunofluorescence histology techniques that included the use of custom antibodies. O. annularis at 5 m water depth exhibited decreased Symbiodiniaceae and increased chromatophore abundances, while O. faveolata at 12 m water depth exhibited inverse relationships. Analysis of seasonal acclimatization of the O. faveolata holobiont in this study, combined with previous reports, suggests that biomolecules are differentially modulated during transition from cooler to warmer SST. Warmer SST was also accompanied by decreased mucus production and decreased Symbiodiniaceae abundance, which is compensated by increased photosynthetic activity enhanced calcification. These interacting processes have facilitated the remarkable resiliency of the corals through geological time.
    Description: Financial support for this work was provided by the Office of Naval Research (N00014-00-1-0609), the Illinois Carl R. Woese Institute for Genomic Biology Undergraduate Summer Research Fellowship, the Illinois Carl R. Woese Institute for Genomic Biology Mark Tracy Fellowship for Translational Research, and the Illinois Department of Molecular and Cellular Biology Jenner Family Summer Research Fellowship and the Edward and Barbara Weil Research Fund provided to the University of Illinois Urbana-Champaign.
    Keywords: Autofluorescence ; Two-photon microscopy ; X-ray Micro CT ; Water Depth ; Bathymetry ; Sea surface temperature ; Symbiodiniaceae symbiont ; chromatophores ; calmodulin ; carbonic anhydrase ; mucus ; Scleractinian corals ; Orbicella annularis ; Orbicella faveolata
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  • 82
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Jenouvrier, S., Judy, C.-C., Wolf, S., Holland, M., Labrousse, S., LaRue, M., Wienecke, B., Fretwell, P., Barbraud, C., Greenwald, N., Stroeve, J., & Trathan, P. N. The call of the emperor penguin: legal responses to species threatened by climate change. Global Change Biology, 27, (2021): 5008– 5029, https://doi.org/10.1111/gcb.15806.
    Description: Species extinction risk is accelerating due to anthropogenic climate change, making it urgent to protect vulnerable species through legal frameworks in order to facilitate conservation actions that help mitigate risk. Here, we discuss fundamental concepts for assessing climate change risks to species using the example of the emperor penguin (Aptenodytes forsteri), currently being considered for protection under the US Endangered Species Act (ESA). This species forms colonies on Antarctic sea ice, which is projected to significantly decline due to ongoing greenhouse gas (GHG) emissions. We project the dynamics of all known emperor penguin colonies under different GHG emission scenarios using a climate-dependent meta-population model including the effects of extreme climate events based on the observational satellite record of colonies. Assessments for listing species under the ESA require information about how species resiliency, redundancy and representation (3Rs) will be affected by threats within the foreseeable future. Our results show that if sea ice declines at the rate projected by climate models under current energy system trends and policies, the 3Rs would be dramatically reduced and almost all colonies would become quasi-extinct by 2100. We conclude that the species should be listed as threatened under the ESA.
    Description: We acknowledge support of NASA (80NSSC20K1289) to SJ, MH, and of NSF—OPP (1744794) to SJ, ML.
    Keywords: climate risk assessments ; Endangered Species Act ; foreseeable future ; population projections ; resiliency, redundancy and representation (3Rs) ; sea ice projections ; species distribution ; treatment of scientific uncertainty
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  • 83
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Johnson, M. D., Scott, J. J., Leray, M., Lucey, N., Bravo, L. M. R., Wied, W. L., & Altieri, A. H. Rapid ecosystem-scale consequences of acute deoxygenation on a Caribbean coral reef. Nature Communications, 12(1), (2021): 4522, https://doi.org/10.1038/s41467-021-24777-3.
    Description: Loss of oxygen in the global ocean is accelerating due to climate change and eutrophication, but how acute deoxygenation events affect tropical marine ecosystems remains poorly understood. Here we integrate analyses of coral reef benthic communities with microbial community sequencing to show how a deoxygenation event rapidly altered benthic community composition and microbial assemblages in a shallow tropical reef ecosystem. Conditions associated with the event precipitated coral bleaching and mass mortality, causing a 50% loss of live coral and a shift in the benthic community that persisted a year later. Conversely, the unique taxonomic and functional profile of hypoxia-associated microbes rapidly reverted to a normoxic assemblage one month after the event. The decoupling of ecological trajectories among these major functional groups following an acute event emphasizes the need to incorporate deoxygenation as an emerging stressor into coral reef research and management plans to combat escalating threats to reef persistence.
    Description: M.D.J. was funded by postdoctoral fellow awards from the Smithsonian Institution’s Marine Global Earth Observatory (MarineGEO) and the Smithsonian Tropical Research Institute (STRI); M.L. and N.L. were funded by postdoctoral support from the STRI Office of Fellowships. J.J.S. was funded by a grant from the Gordon and Betty Moore Foundation awarded to STRI and UC Davis (doi:10.37807/GBMF5603). L.M.R.B., W.L.W., and A.H.A. were supported by MarineGEO, a private funder, and STRI funds to A.H.A. Many of the computations were conducted on the Smithsonian High-Performance Cluster (SI/HPC), Smithsonian Institution (doi:10.25572/SIHPC). We thank Rachel Collin for facilities support at the Bocas del Toro Research Station, Plinio Gondola and the research station staff for logistical support, Roman Barco for insight into the functional analyses, Sherly Castro for informative feedback, and Mike Fox for assistance with community analyses. Research permits were provided by the Autoridad Nacional del Ambiente de Panamá. This paper is the result of research funded by the National Oceanic and Atmospheric Administration’s National Centers for Coastal Ocean Science Competitive Research Program under award NA18NOS4780170 to A.H.A. and M.D.J. through the University of Florida. This is contribution 257 from the Coastal Hypoxia Research Program and 86 from the Smithsonian’s MarineGEO and Tennenbaum Marine Observatories Network.
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  • 84
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Wu, T., Tivey, M. A., Tao, C., Zhang, J., Zhou, F., & Liu, Y. An intermittent detachment faulting system with a large sulfide deposit revealed by multi-scale magnetic surveys. Nature Communications, 12(1), (2021): 5642, https://doi.org/10.1038/s41467-021-25880-1.
    Description: Magmatic and tectonic processes can contribute to discontinuous crustal accretion and play an important role in hydrothermal circulation at ultraslow-spreading ridges, however, it is difficult to accurately describe the processes without an age framework to constrain crustal evolution. Here we report on a multi-scale magnetic survey that provides constraints on the fine-scale evolution of a detachment faulting system that hosts hydrothermal activity at 49.7°E on the Southwest Indian Ridge. Reconstruction of the multi-stage detachment faulting history shows a previous episode of detachment faulting took place 0.76~1.48 My BP, while the present fault has been active for the past ~0.33 My and is just in the prime of life. This fault sustains hydrothermal circulation that has the potential for developing a large sulfide deposit. High resolution multiscale magnetics allows us to constrain the relative balance between periods of detachment faulting and magmatism to better describe accretionary processes on an ultraslow spreading ridge.
    Description: This work was supported by National Key R&D Program of China under contract no. 2018YFC0309901, National Natural Science Foundation of China (41906065), National Key R&D Program of China (2017YFC0306803) and COMRA Major Project under contract no. DY135-S1-01-06 and no. DY135-S1-01-01.
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  • 85
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Li, S., Raychaudhuri, S., Lee, S. A., Brockmann, M. M., Wang, J., Kusick, G., Prater, C., Syed, S., Falahati, H., Ramos, R., Bartol, T. M., Hosy, E., & Watanabe, S. Asynchronous release sites align with NMDA receptors in mouse hippocampal synapses. Nature Communications, 12(1), (2021): 677, https://doi.org/10.1038/s41467-021-21004-x.
    Description: Neurotransmitter is released synchronously and asynchronously following an action potential. Our recent study indicates that the release sites of these two phases are segregated within an active zone, with asynchronous release sites enriched near the center in mouse hippocampal synapses. Here we demonstrate that synchronous and asynchronous release sites are aligned with AMPA receptor and NMDA receptor clusters, respectively. Computational simulations indicate that this spatial and temporal arrangement of release can lead to maximal membrane depolarization through AMPA receptors, alleviating the pore-blocking magnesium leading to greater activation of NMDA receptors. Together, these results suggest that release sites are likely organized to activate NMDA receptors efficiently.
    Description: e also thank the Marine Biological Laboratory and their Neurobiology course for supporting the initial set of experiments (course supported by National Institutes of Health grant R25NS063307). S.W. and this work were supported by start-up funds from the Johns Hopkins University School of Medicine, Johns Hopkins Discovery funds, and the National Science Foundation (1727260), the National Institutes of Health (1DP2 NS111133-01 and 1R01 NS105810-01A1) awarded to S.W. S.W. is an Alfred P. Sloan fellow, McKnight Foundation Scholar, and Klingenstein and Simons Foundation scholar. G.K. was supported by a grant from the National Institutes of Health to the Biochemistry, Cellular and Molecular Biology Program of the Johns Hopkins University School of Medicine (T32 GM007445) and is a National Science Foundation Graduate Research Fellow (2016217537). E.H. and T.M.B. are supported by CRCNS-NIH-ANR grant AMPAR-T. The EM ICE high-pressure freezer was purchased partly with funds from an equipment grant from the National Institutes of Health (S10RR026445) awarded to Scot C Kuo.
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  • 86
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Lenz, P. H., Roncalli, V., Cieslak, M. C., Tarrant, A. M., Castelfranco, A. M., & Hartline, D. K. Diapause vs. reproductive programs: transcriptional phenotypes in a keystone copepod. Communications Biology, 4(1), (2021): 426, https://doi.org/10.1038/s42003-021-01946-0.
    Description: Many arthropods undergo a seasonal dormancy termed “diapause” to optimize timing of reproduction in highly seasonal environments. In the North Atlantic, the copepod Calanus finmarchicus completes one to three generations annually with some individuals maturing into adults, while others interrupt their development to enter diapause. It is unknown which, why and when individuals enter the diapause program. Transcriptomic data from copepods on known programs were analyzed using dimensionality reduction of gene expression and functional analyses to identify program-specific genes and biological processes. These analyses elucidated physiological differences and established protocols that distinguish between programs. Differences in gene expression were associated with maturation of individuals on the reproductive program, while those on the diapause program showed little change over time. Only two of six filters effectively separated copepods by developmental program. The first one included all genes annotated to RNA metabolism and this was confirmed using differential gene expression analysis. The second filter identified 54 differentially expressed genes that were consistently up-regulated in individuals on the diapause program in comparison with those on the reproductive program. Annotated to oogenesis, RNA metabolism and fatty acid biosynthesis, these genes are both indicators for diapause preparation and good candidates for functional studies.
    Description: This work was supported by National Science Foundation Grants (NSF) OCE-1459235 and OCE-1756767 to P.H.L., D.K.H. and AE Christie and OPP-1746087 to A.M.T.
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  • 87
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Eide, M., Zhang, X., Karlsen, O. A., Goldstone, J., Stegeman, J., Jonassen, I., & Goksoyr, A. The chemical defensome of five model teleost fish. Scientific Reports, 11(1), (2021): 10546, https://doi.org/10.1038/s41598-021-89948-0.
    Description: How an organism copes with chemicals is largely determined by the genes and proteins that collectively function to defend against, detoxify and eliminate chemical stressors. This integrative network includes receptors and transcription factors, biotransformation enzymes, transporters, antioxidants, and metal- and heat-responsive genes, and is collectively known as the chemical defensome. Teleost fish is the largest group of vertebrate species and can provide valuable insights into the evolution and functional diversity of defensome genes. We have previously shown that the xenosensing pregnane x receptor (pxr, nr1i2) is lost in many teleost species, including Atlantic cod (Gadus morhua) and three-spined stickleback (Gasterosteus aculeatus), but it is not known if compensatory mechanisms or signaling pathways have evolved in its absence. In this study, we compared the genes comprising the chemical defensome of five fish species that span the teleosteii evolutionary branch often used as model species in toxicological studies and environmental monitoring programs: zebrafish (Danio rerio), medaka (Oryzias latipes), Atlantic killifish (Fundulus heteroclitus), Atlantic cod, and three-spined stickleback. Genome mining revealed evolved differences in the number and composition of defensome genes that can have implication for how these species sense and respond to environmental pollutants, but we did not observe any candidates of compensatory mechanisms or pathways in cod and stickleback in the absence of pxr. The results indicate that knowledge regarding the diversity and function of the defensome will be important for toxicological testing and risk assessment studies.
    Description: The work was supported by the Norwegian Research Council as part of the iCOD and iCOD 2.0 projects (Grant Nos. 192441/I30 and 244654/E40), and the dCod 1.0 project (Grant No. 248840) which is part of Centre for Digital Life Norway. The American collaborators were funded by the National Institute of Health (USA) NIH P42ES007381 (Boston University Superfund Center to JJS and JVG), NIH R21HD073805 (JVG) and NHI R01ES029917 (JVG) grants. The Ocean Outlook exchange program funded the trans-Atlantic collaboration.
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  • 88
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Lapointe, B. E., Brewton, R. A., Herren, L. W., Wang, M., Hu, C., McGillicuddy, D. J., Lindell, S., Hernandez, F. J., & Morton, P. L. Nutrient content and stoichiometry of pelagic Sargassum reflects increasing nitrogen availability in the Atlantic Basin. Nature Communications, 12(1), (2021): 3060, https://doi.org/10.1038/s41467-021-23135-7.
    Description: The pelagic brown macroalgae Sargassum spp. have grown for centuries in oligotrophic waters of the North Atlantic Ocean supported by natural nutrient sources, such as excretions from associated fishes and invertebrates, upwelling, and N2 fixation. Using a unique historical baseline, we show that since the 1980s the tissue %N of Sargassum spp. has increased by 35%, while %P has decreased by 44%, resulting in a 111% increase in the N:P ratio (13:1 to 28:1) and increased P limitation. The highest %N and δ15N values occurred in coastal waters influenced by N-rich terrestrial runoff, while lower C:N and C:P ratios occurred in winter and spring during peak river discharges. These findings suggest that increased N availability is supporting blooms of Sargassum and turning a critical nursery habitat into harmful algal blooms with catastrophic impacts on coastal ecosystems, economies, and human health.
    Description: This work was funded by the US NASA Ocean Biology and Biogeochemistry Program (80NSSC20M0264, NNX16AR74G) and Ecological Forecast Program (NNX17AF57G), NOAA RESTORE Science Program (NA17NOS4510099), National Science Foundation (NSF-OCE 85–15492 and OCE 88–12055), “Save Our Seas” Specialty License Plate funds, granted through the Harbor Branch Oceanographic Institute Foundation, Ft. Pierce, FL, and a Red Wright Fellowship from the Bermuda Biological Station. A portion of this work was performed at the National High Magnetic Field Laboratory, which is supported by National Science Foundation Cooperative Agreement No. DMR-1644779 and the State of Florida. D.J.M. gratefully acknowledges the Holger W. Jannasch and Columbus O’Donnell Iselin Shared Chairs for Excellence in Oceanography, as well as support from the Mill Reef Fund.
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  • 89
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in McPherson, M. L., Finger, D. J., I., Houskeeper, H. F., Bell, T. W., Carr, M. H., Rogers-Bennett, L., & Kudela, R. M. Large-scale shift in the structure of a kelp forest ecosystem co-occurs with an epizootic and marine heatwave. Communications Biology, 4(1), (2021): 298, https://doi.org/10.1038/s42003-021-01827-6.
    Description: Climate change is responsible for increased frequency, intensity, and duration of extreme events, such as marine heatwaves (MHWs). Within eastern boundary current systems, MHWs have profound impacts on temperature-nutrient dynamics that drive primary productivity. Bull kelp (Nereocystis luetkeana) forests, a vital nearshore habitat, experienced unprecedented losses along 350 km of coastline in northern California beginning in 2014 and continuing through 2019. These losses have had devastating consequences to northern California communities, economies, and fisheries. Using a suite of in situ and satellite-derived data, we demonstrate that the abrupt ecosystem shift initiated by a multi-year MHW was preceded by declines in keystone predator population densities. We show strong evidence that northern California kelp forests, while temporally dynamic, were historically resilient to fluctuating environmental conditions, even in the absence of key top predators, but that a series of coupled environmental and biological shifts between 2014 and 2016 resulted in the formation of a persistent, altered ecosystem state with low primary productivity. Based on our findings, we recommend the implementation of ecosystem-based and adaptive management strategies, such as (1) monitoring the status of key ecosystem attributes: kelp distribution and abundance, and densities of sea urchins and their predators, (2) developing management responses to threshold levels of these attributes, and (3) creating quantitative restoration suitability indices for informing kelp restoration efforts.
    Description: M.H.C. received support from the National Science Foundation (OCE‐1538582).
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  • 90
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Lai, C.-Y., Stevens, L. A., Chase, D. L., Creyts, T. T., Behn, M. D., Das, S. B., & Stone, H. A. Hydraulic transmissivity inferred from ice-sheet relaxation following Greenland supraglacial lake drainages. Nature Communications, 12(1), (2021): 3955, https://doi.org/10.1038/s41467-021-24186-6.
    Description: Surface meltwater reaching the base of the Greenland Ice Sheet transits through drainage networks, modulating the flow of the ice sheet. Dye and gas-tracing studies conducted in the western margin sector of the ice sheet have directly observed drainage efficiency to evolve seasonally along the drainage pathway. However, the local evolution of drainage systems further inland, where ice thicknesses exceed 1000 m, remains largely unknown. Here, we infer drainage system transmissivity based on surface uplift relaxation following rapid lake drainage events. Combining field observations of five lake drainage events with a mathematical model and laboratory experiments, we show that the surface uplift decreases exponentially with time, as the water in the blister formed beneath the drained lake permeates through the subglacial drainage system. This deflation obeys a universal relaxation law with a timescale that reveals hydraulic transmissivity and indicates a two-order-of-magnitude increase in subglacial transmissivity (from 0.8 ± 0.3 mm3 to 215 ± 90.2 mm3) as the melt season progresses, suggesting significant changes in basal hydrology beneath the lakes driven by seasonal meltwater input.
    Description: C.-Y.L. and L.A.S thank Lamont-Doherty Earth Observatory for funding through the Lamont Postdoctoral Fellowships. D.L.C acknowledges support from the National Science Foundation (NSF) Graduate Research Fellowship. T.T.C. was supported by NSF’s Office of Polar Programs (NSF-OPP) through OPP-1643970, the National Aeronautics and Space Administration (NASA) through NNX16AJ95G, and a grant from the Vetlesen Foundation. S.B.D. and M.D.B. acknowledge funding from NSF-OPP and NASA’s Cryospheric Sciences Program through OPP-1838410, ARC-1023364, ARC-0520077, and NNX10AI30G. H.A.S. thanks the High Meadows Environmental Institute and the Carbon Mitigation Initiative at Princeton University. This publication was supported by the Princeton University Library Open Access Fund.
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  • 91
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Lin, Y., Moreno, C., Marchetti, A., Ducklow, H., Schofield, O., Delage, E., Meredith, M., Li, Z., Eveillard, D., Chaffron, S., & Cassar, N. Decline in plankton diversity and carbon flux with reduced sea ice extent along the Western Antarctic Peninsula. Nature Communications, 12(1), (2021): 4948, https://doi.org/10.1038/s41467-021-25235-w.
    Description: Since the middle of the past century, the Western Antarctic Peninsula has warmed rapidly with a significant loss of sea ice but the impacts on plankton biodiversity and carbon cycling remain an open question. Here, using a 5-year dataset of eukaryotic plankton DNA metabarcoding, we assess changes in biodiversity and net community production in this region. Our results show that sea-ice extent is a dominant factor influencing eukaryotic plankton community composition, biodiversity, and net community production. Species richness and evenness decline with an increase in sea surface temperature (SST). In regions with low SST and shallow mixed layers, the community was dominated by a diverse assemblage of diatoms and dinoflagellates. Conversely, less diverse plankton assemblages were observed in waters with higher SST and/or deep mixed layers when sea ice extent was lower. A genetic programming machine-learning model explained up to 80% of the net community production variability at the Western Antarctic Peninsula. Among the biological explanatory variables, the sea-ice environment associated plankton assemblage is the best predictor of net community production. We conclude that eukaryotic plankton diversity and carbon cycling at the Western Antarctic Peninsula are strongly linked to sea-ice conditions.
    Description: This work is supported by NSF OPP-1643534 to N.C., NSF OPP-1341479 to A.M., and NSF PLR-1440435 to H.D. and O.S. (Palmer LTER).
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  • 92
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Keikhosravi, A., Shribak, M., Conklin, M. W., Liu, Y., Li, B., Loeffler, A., Levenson, R. M., & Eliceiri, K. W. Real-time polarization microscopy of fibrillar collagen in histopathology. Scientific Reports, 11(1), (2021): 19063, https://doi.org/10.1038/s41598-021-98600-w.
    Description: Over the past two decades, fibrillar collagen reorganization parameters such as the amount of collagen deposition, fiber angle and alignment have been widely explored in numerous studies. These parameters are now widely accepted as stromal biomarkers and linked to disease progression and survival time in several cancer types. Despite all these advances, there has not been a significant effort to make it possible for clinicians to explore these biomarkers without adding steps to the clinical workflow or by requiring high-cost imaging systems. In this paper, we evaluate previously described polychromatic polarization microscope (PPM) to visualize collagen fibers with an optically generated color representation of fiber orientation and alignment when inspecting the sample by a regular microscope with minor modifications. This system does not require stained slides, but is compatible with histological stains such as H&E. Consequently, it can be easily accommodated as part of regular pathology review of tissue slides, while providing clinically useful insight into stromal composition.
    Description: This work was supported by NIH R01 CA238191 (KWE), NIH P41GM135019 (KWE), NIH R01 GM101701 (MS), funding from the Morgridge Institute for Research (KWE), the Semiconductor Research Corporation (SRC) (KWE), and the William T. Golden Endowment (MS).
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  • 93
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Brun, N. R., Panlilio, J. M., Zhang, K., Zhao, Y., Ivashkin, E., Stegeman, J. J., & Goldstone, J. Developmental exposure to non-dioxin-like polychlorinated biphenyls promotes sensory deficits and disrupts dopaminergic and GABAergic signaling in zebrafish. Communications Biology, 4(1), (2021): 1129, https://doi.org/10.1038/s42003-021-02626-9.
    Description: The most abundant polychlorinated biphenyl (PCB) congeners found in the environment and in humans are neurotoxic. This is of particular concern for early life stages because the exposure of the more vulnerable developing nervous system to neurotoxic chemicals can result in neurobehavioral disorders. In this study, we uncover currently unknown links between PCB target mechanisms and neurobehavioral deficits using zebrafish as a vertebrate model. We investigated the effects of the abundant non-dioxin-like (NDL) congener PCB153 on neuronal morphology and synaptic transmission linked to the proper execution of a sensorimotor response. Zebrafish that were exposed during development to concentrations similar to those found in human cord blood and PCB contaminated sites showed a delay in startle response. Morphological and biochemical data demonstrate that even though PCB153-induced swelling of afferent sensory neurons, the disruption of dopaminergic and GABAergic signaling appears to contribute to PCB-induced motor deficits. A similar delay was observed for other NDL congeners but not for the potent dioxin-like congener PCB126. The effects on important and broadly conserved signaling mechanisms in vertebrates suggest that NDL PCBs may contribute to neurodevelopmental abnormalities in humans and increased selection pressures in vertebrate wildlife.
    Description: This work was supported by the Swiss National Science Foundation P2EZP2_165200 (NRB), the Boston University Superfund Research Program NIH 5P42ES007381 (J.J.S. and J.V.G.), the Woods Hole Center for Oceans and Human Health (NIH: P01ES021923 and P01ES028938; NSF: OCE-1314642 and OCE-1840381) (N.R.B., J.M.P., and J.J.S.), and the National Natural Science Foundation of China 22006099 (K.Z. and Y.Z.) and the Shanghai Pujiang Program 19PJ1404900 (K.Z. and Y.Z.).
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  • 94
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Sauvage, J. F., Flinders, A., Spivack, A. J., Pockalny, R., Dunlea, A. G., Anderson, C. H., Smith, D. C., Murray, R. W., & D'Hondt, S. The contribution of water radiolysis to marine sedimentary life. Nature Communications, 12(1), (2021): 1297, https://doi.org/10.1038/s41467-021-21218-z.
    Description: Water radiolysis continuously produces H2 and oxidized chemicals in wet sediment and rock. Radiolytic H2 has been identified as the primary electron donor (food) for microorganisms in continental aquifers kilometers below Earth’s surface. Radiolytic products may also be significant for sustaining life in subseafloor sediment and subsurface environments of other planets. However, the extent to which most subsurface ecosystems rely on radiolytic products has been poorly constrained, due to incomplete understanding of radiolytic chemical yields in natural environments. Here we show that all common marine sediment types catalyse radiolytic H2 production, amplifying yields by up to 27X relative to pure water. In electron equivalents, the global rate of radiolytic H2 production in marine sediment appears to be 1-2% of the global organic flux to the seafloor. However, most organic matter is consumed at or near the seafloor, whereas radiolytic H2 is produced at all sediment depths. Comparison of radiolytic H2 consumption rates to organic oxidation rates suggests that water radiolysis is the principal source of biologically accessible energy for microbial communities in marine sediment older than a few million years. Where water permeates similarly catalytic material on other worlds, life may also be sustained by water radiolysis.
    Description: This project was funded by the US National Science Foundation (through grant NSF-OCE-1130735 and the Center for Deep Dark Energy Biosphere Investigations [C-DEBI; grant NSF-OCE-0939564]); the National Aeronautics and Space Administration (grant NNX12AD65G); the U.S. Science Support Program, IODP; and a Schlanger Ocean Drilling Fellowship to J.F.S. This is a contribution to the Deep Carbon Observatory (DCO). It is C-DEBI publication 553.
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  • 95
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Hawkings, J. R., Linhoff, B. S., Wadham, J. L., Stibal, M., Lamborg, C. H., Carling, G. T., Lamarche-Gagnon, G., Kohler, T. J., Ward, R., Hendry, K. R., Falteisek, L., Kellerman, A. M., Cameron, K. A., Hatton, J. E., Tingey, S., Holt, A. D., Vinsova, P., Hofer, S., Bulinova, M., Větrovský, T., Meire, L., Spencer, R. G. M. Large subglacial source of mercury from the southwestern margin of the Greenland Ice Sheet. Nature Geoscience, 14, (2021): 496-502, https://doi.org/10.1038/s41561-021-00753-w.
    Description: The Greenland Ice Sheet is currently not accounted for in Arctic mercury budgets, despite large and increasing annual runoff to the ocean and the socio-economic concerns of high mercury levels in Arctic organisms. Here we present concentrations of mercury in meltwaters from three glacial catchments on the southwestern margin of the Greenland Ice Sheet and evaluate the export of mercury to downstream fjords based on samples collected during summer ablation seasons. We show that concentrations of dissolved mercury are among the highest recorded in natural waters and mercury yields from these glacial catchments (521–3,300 mmol km−2 year−1) are two orders of magnitude higher than from Arctic rivers (4–20 mmol km−2 year−1). Fluxes of dissolved mercury from the southwestern region of Greenland are estimated to be globally significant (15.4–212 kmol year−1), accounting for about 10% of the estimated global riverine flux, and include export of bioaccumulating methylmercury (0.31–1.97 kmol year−1). High dissolved mercury concentrations (~20 pM inorganic mercury and ~2 pM methylmercury) were found to persist across salinity gradients of fjords. Mean particulate mercury concentrations were among the highest recorded in the literature (~51,000 pM), and dissolved mercury concentrations in runoff exceed reported surface snow and ice values. These results suggest a geological source of mercury at the ice sheet bed. The high concentrations of mercury and its large export to the downstream fjords have important implications for Arctic ecosystems, highlighting an urgent need to better understand mercury dynamics in ice sheet runoff under global warming.
    Description: This research is part of a European Commission Horizon 2020 Marie Skłodowska-Curie Actions fellowship ICICLES (grant agreement #793962) to J.R.H. Greenland terrestrial research campaigns were funded by a UK NERC standard grant (NE/I008845/1) and a Leverhulme Trust Research Grant (RPG-2016-439) to J.L.W., with additional support provided by a Royal Society Wolfson Merit Award to J.L.W. Additional funding came from Czech Science Foundation grants (GACR; 15-17346Y and 18-12630S) to M.S. Fjord fieldwork was supported by European Research Council grant ICY-LAB (grant agreement 678371) and Royal Society Enhancement Award (grant RGF\EA\181036) to K.R.H. L.M. was funded by research programme VENI (0.16.Veni.192.150, NWO). T.J.K. was supported by Charles University Research Centre program no. 204069. The authors thank the captain and crew of the RV Kisaq and staff at the Greenland Institute of Natural Resources for assistance during fjord fieldwork, and all those involved with fieldwork at Leverett Camp during the 2012 and 2015 field campaigns. M. Cooper is thanked for providing the geological overview file for Extended Data Fig. 1a, and K. Mankoff for help in generating the modelled GrIS discharge datasets. The authors also thank G. White in the geochemistry group at the National High Magnetic Field Geochemistry Laboratory, which is supported by NSF DMR-1644779 and the State of Florida, for analytical support.
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  • 96
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Li, F., Lozier, M. S., Bacon, S., Bower, A. S., Cunningham, S. A., de Jong, M. F., DeYoung, B., Fraser, N., Fried, N., Han, G., Holliday, N. P., Holte, J., Houpert, L., Inall, M. E., Johns, W. E., Jones, S., Johnson, C., Karstensen, J., Le Bras, I. A., P. Lherminier, X. Lin, H. Mercier, M. Oltmanns, A. Pacini, T. Petit, R. S. Pickart, D. Rayner, F. Straneo, V. Thierry, M. Visbeck, I. Yashayaev & Zhou, C. Subpolar North Atlantic western boundary density anomalies and the Meridional Overturning Circulation. Nature Communications, 12(1), (2021): 3002, https://doi.org/10.1038/s41467-021-23350-2.
    Description: Changes in the Atlantic Meridional Overturning Circulation, which have the potential to drive societally-important climate impacts, have traditionally been linked to the strength of deep water formation in the subpolar North Atlantic. Yet there is neither clear observational evidence nor agreement among models about how changes in deep water formation influence overturning. Here, we use data from a trans-basin mooring array (OSNAP—Overturning in the Subpolar North Atlantic Program) to show that winter convection during 2014–2018 in the interior basin had minimal impact on density changes in the deep western boundary currents in the subpolar basins. Contrary to previous modeling studies, we find no discernable relationship between western boundary changes and subpolar overturning variability over the observational time scales. Our results require a reconsideration of the notion of deep western boundary changes representing overturning characteristics, with implications for constraining the source of overturning variability within and downstream of the subpolar region.
    Description: We acknowledge funding from the Physical Oceanography Program of the U.S. National Science Foundation (OCE-1259398, OCE-1756231, OCE-1948335); the U.K. Natural Environment Research Council (NERC) National Capability programs the Extended Ellett Line and CLASS (NE/R015953/1), and NERC grants UK-OSNAP (NE/K010875/1, NE/K010875/2, NE/K010700/1) and U.K. OSNAP Decade (NE/T00858X/1, NE/T008938/1). Additional support was received from the European Union 7th Framework Program (FP7 2007-2013) under grant 308299 (NACLIM), the Horizon 2020 research and innovation program under grants 727852 (Blue-Action), 862626 (EuroSea). We also acknowledge support from the Royal Netherlands Institute for Sea Research, the Surface Water and Ocean Topography-Canada (SWOT-C), Canadian Space Agency, the Aquatic Climate Change Adaptation Services Program (ACCASP), Fisheries and Oceans Canada, an Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant, and from the China’s national key research and development projects (2016YFA0601803), the National Natural Science Foundation of China (41925025) and the Fundamental Research Funds for the Central Universities (201424001). Support for the 53°N array by the RACE program of the German Ministry BMBF is acknowledged, as is the contribution from Fisheries and Oceans Canada’s Atlantic Zone Monitoring Program.
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  • 97
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Pankov, K., McArthur, A. G., Gold, D. A., Nelson, D. R., Goldstone, J., & Wilson, J. Y. The cytochrome P450 (CYP) superfamily in cnidarians. Scientific Reports, 11(1), (2021): 9834, https://doi.org/10.1038/s41598-021-88700-y.
    Description: The cytochrome P450 (CYP) superfamily is a diverse and important enzyme family, playing a central role in chemical defense and in synthesis and metabolism of major biological signaling molecules. The CYPomes of four cnidarian genomes (Hydra vulgaris, Acropora digitifera, Aurelia aurita, Nematostella vectensis) were annotated; phylogenetic analyses determined the evolutionary relationships amongst the sequences and with existing metazoan CYPs. 155 functional CYPs were identified and 90 fragments. Genes were from 24 new CYP families and several new subfamilies; genes were in 9 of the 12 established metazoan CYP clans. All species had large expansions of clan 2 diversity, with H. vulgaris having reduced diversity for both clan 3 and mitochondrial clan. We identified potential candidates for xenobiotic metabolism and steroidogenesis. That each genome contained multiple, novel CYP families may reflect the large evolutionary distance within the cnidarians, unique physiology in the cnidarian classes, and/or different ecology of the individual species.
    Description: This study was supported by grants from the Natural Science Engineering Research Council of Canada in the form of a Discovery Grant (RGPIN-328204-2011 and RGPIN-05767-2016) to J.Y.W. A.G.M. held a Cisco Research Chair in Bioinformatics, supported by Cisco Systems Canada, Inc. K.V.P. was supported by a Natural Sciences and Engineering Research Council of Canada (NSERC) Undergraduate Student Research Award (USRA). Computer resources were supplied by the McMaster Service Lab and Repository computing cluster, funded in part by grants to A.G.M. from the Canadian Foundation for Innovation (34531 to A.G.M.).
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  • 98
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Zhang, M., Guo, Z., Xu, S., Barry, P. H., Sano, Y., Zhang, L., Halldorsson, S. A., Chen, A.-T., Cheng, Z., Liu, C.-Q., Li, S.-L., Lang, Y.-C., Zheng, G., Li, Z., Li, L., & Li, Y. Linking deeply-sourced volatile emissions to plateau growth dynamics in southeastern Tibetan Plateau. Nature Communications, 12(1), (2021): 4157, https://doi.org/10.1038/s41467-021-24415-y.
    Description: The episodic growth of high-elevation orogenic plateaux is controlled by a series of geodynamic processes. However, determining the underlying mechanisms that drive plateau growth dynamics over geological history and constraining the depths at which growth originates, remains challenging. Here we present He-CO2-N2 systematics of hydrothermal fluids that reveal the existence of a lithospheric-scale fault system in the southeastern Tibetan Plateau, whereby multi-stage plateau growth occurred in the geological past and continues to the present. He isotopes provide unambiguous evidence for the involvement of mantle-scale dynamics in lateral expansion and localized surface uplift of the Tibetan Plateau. The excellent correlation between 3He/4He values and strain rates, along the strike of Indian indentation into Asia, suggests non-uniform distribution of stresses between the plateau boundary and interior, which modulate southeastward growth of the Tibetan Plateau within the context of India-Asia convergence. Our results demonstrate that deeply-sourced volatile geochemistry can be used to constrain deep dynamic processes involved in orogenic plateau growth.
    Description: This work was supported by China Seismic Experimental Site (CSES) (2019CSES0104), the Strategic Priority Research Program (B) of Chinese Academy of Sciences (XDB26000000), the National Key Research and Development Program of China (2020YFA0607700), the National Natural Science Foundation of China (41930642, 41602341, 41772355, and 41702361), the Second Tibetan Plateau Scientific Expedition and Research Program (STEP) (2019QZKK0702), and the United Laboratory of High-Pressure Physics and Earthquake Science (2019HPPES02). P.H.B. was supported by the US National Science Foundation EAR Grant 1144559 during a portion of this work.
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  • 99
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Perez, E., Ryan, S., Andres, M., Gawarkiewicz, G., Ummenhofer, C. C., Bane, J., & Haines, S. Understanding physical drivers of the 2015/16 marine heatwaves in the Northwest Atlantic. Scientific Reports, 11(1), (2021): 17623, https://doi.org/10.1038/s41598-021-97012-0.
    Description: The Northwest Atlantic, which has exhibited evidence of accelerated warming compared to the global ocean, also experienced several notable marine heatwaves (MHWs) over the last decade. We analyze spatiotemporal patterns of surface and subsurface temperature structure across the Northwest Atlantic continental shelf and slope to assess the influences of atmospheric and oceanic processes on ocean temperatures. Here we focus on MHWs from 2015/16 and examine their physical drivers using observational and reanalysis products. We find that a combination of jet stream latitudinal position and ocean advection, mainly due to warm core rings shed by the Gulf Stream, plays a role in MHW development. While both atmospheric and oceanic drivers can lead to MHWs they have different temperature signatures with each affecting the vertical structure differently and horizontal spatial patterns of a MHW. Northwest Atlantic MHWs have significant socio-economic impacts and affect commercially important species such as squid and lobster.
    Description: The work was supported by a Woods Hole Oceanographic Institution Summer Student Fellowship (to E.P.) through the National Science Foundation (NSF) REU under grant OCE-1852460, a Feodor-Lynen Fellowship by the Alexander von Humboldt Foundation and the WHOI Postdoctoral Scholar program (to S.R.), NSF grants OCE-1924041 (to M.A.) and OCE-1851261 (to G.G.) and OCE-1558920 (to J.B. and H. Seim at the Univ. of North Carolina), ONR grant N-14-19-1-2646 (to G.G.), and the James E. and Barbara V. Moltz Fellowship for Climate-Related Research (to C.C.U.).
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  • 100
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Hawk, J. D., Wisdom, E. M., Sengupta, T., Kashlan, Z. D., & Colon-Ramos, D. A. A genetically encoded tool for reconstituting synthetic modulatory neurotransmission and reconnect neural circuits in vivo. Nature Communications, 12(1), (2021): 4795, https://doi.org/10.1038/s41467-021-24690-9.
    Description: Chemogenetic and optogenetic tools have transformed the field of neuroscience by facilitating the examination and manipulation of existing circuits. Yet, the field lacks tools that enable rational rewiring of circuits via the creation or modification of synaptic relationships. Here we report the development of HySyn, a system designed to reconnect neural circuits in vivo by reconstituting synthetic modulatory neurotransmission. We demonstrate that genetically targeted expression of the two HySyn components, a Hydra-derived neuropeptide and its receptor, creates de novo neuromodulatory transmission in a mammalian neuronal tissue culture model and functionally rewires a behavioral circuit in vivo in the nematode Caenorhabditis elegans. HySyn can interface with existing optogenetic, chemogenetic and pharmacological approaches to functionally probe synaptic transmission, dissect neuropeptide signaling, or achieve targeted modulation of specific neural circuits and behaviors.
    Description: This work was initiated in the Grass Laboratory at the Marine Biological Laboratories (MBL) with funding through a Grass Fellowship awarded to J.D.H. Thanks to Richard Goodman (OHSU) for encouragement during the conceptualization of the fellowship application, and the 2019 Grass Fellows, Mel Coleman (Grass Director), and Christophe Dupré (Associate Director) for advice and support during the summer fellowship. We thank the MBL Division of Education and participants in the Vendor Equipment Loan Program. Special thanks to Sutter Instruments, who generously provided all electrophysiology equipment and substantial on-site assistance, and Zeiss, who provided on-site assistance at MBL. We thank Zhao-Wen Wang and Ping Liu (UConn) for guidance and training in patch-clamp electrophysiology, as well as providing Neuro2a cells. We thank Rob Steele (UCI) for supplying Hydra, as well as advice and inspiration on Hydra biology. We thank members of the Colón-Ramos lab and Hari Shroff (NIH) for thoughtful comments on the manuscript. We thank Michael Koelle and Andrew Olson (Yale University) for advice and reagents regarding serotonin rewiring experiments. We also thank Steve Flavell (MIT) for ideas and reagents regarding the experiments associated with del-7. We thank Life Science Editors for editing assistance. D.A.C.-R. is an MBL Fellow. Research in the D.A.C.-R. lab was supported by NIH R01NS076558, DP1NS111778, and by an HHMI Scholar Award.
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