Publication Date:
1993-12-10
Description:
Peripheral blood mononuclear cells (PBMCs) from many asymptomatic individuals infected with human immunodeficiency virus-type 1 (HIV) are unresponsive as measured by in vitro T cell proliferation and interleukin-2 (IL-2) production to influenza virus and synthetic peptides of HIV envelope (Env). Strong influenza virus- and Env-stimulated IL-2 responses and T cell proliferation were restored when cultures were stimulated in the presence of IL-12. Interferon-gamma production by PBMCs from HIV seropositive (HIV+) patients was also restored with IL-12. Furthermore, in vitro antigen-specific production of IL-2 and proliferation of PBMCs from HIV- donors were suppressed by antibody to IL-12, but were not enhanced by addition of exogenous IL-12. Thus, IL-12 may be limiting in PBMCs from HIV+ but not HIV- individuals. These findings demonstrate that IL-12 can restore HIV-specific cell-mediated immunity in vitro in HIV-infected individuals and suggest a potential use of IL-12 in augmenting the diminished immunologic functions associated with HIV infection.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Clerici, M -- Lucey, D R -- Berzofsky, J A -- Pinto, L A -- Wynn, T A -- Blatt, S P -- Dolan, M J -- Hendrix, C W -- Wolf, S F -- Shearer, G M -- New York, N.Y. -- Science. 1993 Dec 10;262(5140):1721-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Experimental Immunology Branch, National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD 20892.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7903123" target="_blank"〉PubMed〈/a〉
Keywords:
Cells, Cultured
;
Gene Products, env/immunology
;
HIV Infections/*immunology
;
HIV-1/*immunology
;
Humans
;
Immunity, Cellular
;
Influenza A virus/immunology
;
Interferon-gamma/biosynthesis
;
Interleukin-12
;
Interleukin-2/biosynthesis/pharmacology
;
Interleukins/immunology/*pharmacology
;
Killer Cells, Natural/immunology
;
Leukocytes, Mononuclear/immunology
;
Lymphocyte Activation
;
Phytohemagglutinins/immunology
;
Recombinant Proteins/pharmacology
;
T-Lymphocytes, Helper-Inducer/*immunology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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