Publikationsdatum:
1993-02-05
Beschreibung:
Introduction of TCR alpha transgene, TCR beta transgene, or both into RAG-2-/-mice differentially rescues T cell development. RAG-2-/- mice have small numbers of TCR-CD4-CD8-(double negative, DN) thymocytes that express CD3 gamma delta epsilon and zeta proteins intracellularly. Introduction of a TCR beta transgene, but not a TCR alpha transgene, into the RAG-2-/- background restored normal numbers of thymocytes. These cells were CD4+CD8+ (double positive, DP) and expressed small amounts of surface TCR beta chain dimers in association with CD3 gamma delta epsilon but not zeta. RAG-2-/- mice that expressed alpha and beta TCR transgenes developed both DP and single positive thymocytes. Thus, the TCR beta subunit, possibly in association with a novel CD3 complex, participates in the DN to the DP transition.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shinkai, Y -- Koyasu, S -- Nakayama, K -- Murphy, K M -- Loh, D Y -- Reinherz, E L -- Alt, F W -- AI19807/AI/NIAID NIH HHS/ -- AI20047/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 1993 Feb 5;259(5096):822-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Children's Hospital, Boston, MA 02115.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8430336" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Animals
;
Antibodies, Monoclonal
;
Antigens, CD3/analysis/*genetics
;
Antigens, CD4/analysis
;
Antigens, CD8/analysis
;
Base Sequence
;
Cell Membrane/immunology
;
*DNA-Binding Proteins
;
Electrophoresis, Polyacrylamide Gel
;
Gene Expression
;
Mice
;
Mice, Transgenic
;
Molecular Sequence Data
;
Molecular Weight
;
Oligodeoxyribonucleotides
;
Polymerase Chain Reaction/methods
;
Proteins/*genetics
;
Receptors, Antigen, T-Cell, alpha-beta/analysis/*genetics
;
Receptors, Antigen, T-Cell, gamma-delta/analysis/*genetics
;
T-Lymphocyte Subsets/immunology
;
T-Lymphocytes/*immunology
;
Thymus Gland/immunology
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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