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  • History, 20th Century  (177)
  • Protein Binding  (130)
  • History, Ancient  (96)
  • Nature Publishing Group (NPG)  (389)
  • Krefeld : Geologischer Dienst Nordhein-Westfalen
  • Irkutsk : Ross. Akad. Nauk, Sibirskoe Otd., Inst. Zemnoj Kory
  • 2005-2009  (389)
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  • 1
    Publikationsdatum: 2009-01-14
    Beschreibung: 〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340503/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340503/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kelly, Bernard T -- McCoy, Airlie J -- Spate, Kira -- Miller, Sharon E -- Evans, Philip R -- Honing, Stefan -- Owen, David J -- 090909/Wellcome Trust/United Kingdom -- MC_U105178845/Medical Research Council/United Kingdom -- England -- Nature. 2008 Dec 18;456(7224):976-79. doi: 10.1038/nature07422.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19140243" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Adaptor Protein Complex 2/*chemistry/genetics/*metabolism ; Amino Acid Motifs ; Animals ; Antigens, CD4/*chemistry/*metabolism ; Binding Sites ; Conserved Sequence ; *Endocytosis ; Humans ; Leucine/*metabolism ; Mice ; Models, Molecular ; Protein Binding ; Protein Conformation ; Protein Subunits/chemistry/genetics/metabolism ; Rats
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 2
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-01-17
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dalton, Rex -- England -- Nature. 2009 Jan 15;457(7227):241. doi: 10.1038/457241a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19148062" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Conservation of Energy Resources/*trends ; History, 20th Century ; History, 21st Century ; United States ; United States Government Agencies/*organization & administration
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 3
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-09-11
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Goode, Scott -- England -- Nature. 2009 Sep 10;461(7261):167. doi: 10.1038/461167d.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19741681" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): History, 17th Century ; History, 18th Century ; History, 19th Century ; History, 20th Century ; *Religion and Science ; Research Personnel/*history/psychology/standards
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    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 4
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-04-01
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dalton, Rex -- Witze, Alexandra -- England -- Nature. 2009 Mar 26;458(7237):396. doi: 10.1038/458396a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19334300" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Ecosystem ; *Greenhouse Effect ; History, 20th Century ; History, 21st Century ; Leadership ; Marine Biology ; United States ; United States Government Agencies/*organization & administration
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 5
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-02-20
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sunami, Atsushi -- Kurokawa, Kiyoshi -- England -- Nature. 2009 Feb 19;457(7232):960-1. doi: 10.1038/457960a.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉National Graduate Institute for Policy Studies in Tokyo. sunami-atsushi@grips.ac.jp〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19225502" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Emigrants and Immigrants ; Financing, Government/history ; Foreign Professional Personnel ; History, 20th Century ; History, 21st Century ; International Cooperation ; Japan ; *Public Policy ; Research/economics/*history/manpower/*trends
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 6
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-12-25
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kayani, Saheeb Ahmed -- England -- Nature. 2009 Dec 24;462(7276):984. doi: 10.1038/462984b.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20033020" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): *Biological Evolution ; History, 20th Century ; History, 21st Century ; Humans ; Pakistan ; *Religion and Science ; Science/history
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 7
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-06-19
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Reiff, Sarah B -- Striepen, Boris -- England -- Nature. 2009 Jun 18;459(7249):918-9. doi: 10.1038/459918a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19536248" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Humans ; Malaria, Falciparum/drug therapy/*parasitology ; Models, Biological ; Plasmodium falciparum/*metabolism ; Protein Binding ; Protein Transport ; Protozoan Proteins/antagonists & inhibitors/*metabolism ; Vacuoles/metabolism/parasitology
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 8
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-10-16
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Swaminathan, M S -- England -- Nature. 2009 Oct 15;461(7266):894. doi: 10.1038/461894a.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉M. S. Swaminathan Research Foundation, Third Cross Street, Taramani Institutional Area, Chennai 600 113, India. chairman@mssrf.res.in〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19829366" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Agriculture/*history ; Breeding/history ; Food Supply/history ; History, 20th Century ; History, 21st Century ; Humans ; Plant Diseases/genetics/microbiology ; Triticum/genetics/physiology
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 9
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-06-19
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉McDonnell, Anna -- England -- Nature. 2009 Jun 18;459(7249):909. doi: 10.1038/459909b.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19536241" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Female ; Fertility ; History, Ancient ; Humans ; Pregnancy ; Sculpture/*history
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 10
    Publikationsdatum: 2009-07-31
    Beschreibung: P2X receptors are cation-selective ion channels gated by extracellular ATP, and are implicated in diverse physiological processes, from synaptic transmission to inflammation to the sensing of taste and pain. Because P2X receptors are not related to other ion channel proteins of known structure, there is at present no molecular foundation for mechanisms of ligand-gating, allosteric modulation and ion permeation. Here we present crystal structures of the zebrafish P2X(4) receptor in its closed, resting state. The chalice-shaped, trimeric receptor is knit together by subunit-subunit contacts implicated in ion channel gating and receptor assembly. Extracellular domains, rich in beta-strands, have large acidic patches that may attract cations, through fenestrations, to vestibules near the ion channel. In the transmembrane pore, the 'gate' is defined by an approximately 8 A slab of protein. We define the location of three non-canonical, intersubunit ATP-binding sites, and suggest that ATP binding promotes subunit rearrangement and ion channel opening.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720809/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720809/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kawate, Toshimitsu -- Michel, Jennifer Carlisle -- Birdsong, William T -- Gouaux, Eric -- U54 GM075026/GM/NIGMS NIH HHS/ -- U54 GM075026-04/GM/NIGMS NIH HHS/ -- Howard Hughes Medical Institute/ -- England -- Nature. 2009 Jul 30;460(7255):592-8. doi: 10.1038/nature08198.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Vollum Institute, Oregon Health and Science University, 3181 Southwest Sam Jackson Park Road, Oregon 97239, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19641588" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Adenosine Triphosphate/metabolism ; Animals ; Binding Sites ; Cell Line ; Crystallography, X-Ray ; Gadolinium/metabolism ; Humans ; Ion Channels/antagonists & inhibitors/*chemistry ; Membrane Proteins/chemistry ; *Models, Molecular ; Protein Binding ; Protein Folding ; Protein Structure, Tertiary ; Purinergic P2 Receptor Antagonists ; Receptors, Purinergic P2/*chemistry ; Receptors, Purinergic P2X4 ; Zebrafish/*physiology ; Zebrafish Proteins/antagonists & inhibitors/*chemistry
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 11
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-06-26
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rensberger, Boyce -- England -- Nature. 2009 Jun 25;459(7250):1055-6. doi: 10.1038/4591055a.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Knight Science Journalism Fellowship programme at the Massachusetts Institute of Technology in Cambridge. boycerensberger@gmail.com〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19553977" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): History, 19th Century ; History, 20th Century ; History, 21st Century ; Journalism/history/standards/*trends ; Periodicals as Topic ; *Science
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 12
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-02-25
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Goudsmit, Jaap -- England -- Nature. 2009 Jan 22;457(7228):394. doi: 10.1038/457394a.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Jaap Goudsmit is in the Research and Development Department of Crucell Holland, PO Box 2048, Leiden, 2301 CA, the Netherlands, and in the Academic Medical Center of the University of Amsterdam. j.goudsmit@crucell.com.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19158783" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; History, 20th Century ; History, 21st Century ; Humans ; Nobel Prize ; Prion Diseases/*history/transmission ; Prions/chemistry/*history/metabolism
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 13
    Publikationsdatum: 2009-07-31
    Beschreibung: Reactive oxygen species (ROS) produced by NADPH oxidase function as defence and signalling molecules related to innate immunity and various cellular responses. The activation of NADPH oxidase in response to plasma membrane receptor activation depends on the phosphorylation of cytoplasmic oxidase subunits, their translocation to membranes and the assembly of all NADPH oxidase components. Tumour necrosis factor (TNF) is a prominent stimulus of ROS production, but the molecular mechanisms by which TNF activates NADPH oxidase are poorly understood. Here we identify riboflavin kinase (RFK, formerly known as flavokinase) as a previously unrecognized TNF-receptor-1 (TNFR1)-binding protein that physically and functionally couples TNFR1 to NADPH oxidase. In mouse and human cells, RFK binds to both the TNFR1-death domain and to p22(phox), the common subunit of NADPH oxidase isoforms. RFK-mediated bridging of TNFR1 and p22(phox) is a prerequisite for TNF-induced but not for Toll-like-receptor-induced ROS production. Exogenous flavin mononucleotide or FAD was able to substitute fully for TNF stimulation of NADPH oxidase in RFK-deficient cells. RFK is rate-limiting in the synthesis of FAD, an essential prosthetic group of NADPH oxidase. The results suggest that TNF, through the activation of RFK, enhances the incorporation of FAD in NADPH oxidase enzymes, a critical step for the assembly and activation of NADPH oxidase.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yazdanpanah, Benjamin -- Wiegmann, Katja -- Tchikov, Vladimir -- Krut, Oleg -- Pongratz, Carola -- Schramm, Michael -- Kleinridders, Andre -- Wunderlich, Thomas -- Kashkar, Hamid -- Utermohlen, Olaf -- Bruning, Jens C -- Schutze, Stefan -- Kronke, Martin -- England -- Nature. 2009 Aug 27;460(7259):1159-63. doi: 10.1038/nature08206. Epub 2009 Jul 29.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19641494" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Cell Line ; Cytochrome b Group/metabolism ; Enzyme Activation ; Fibroblasts ; Flavin Mononucleotide/metabolism ; Flavin-Adenine Dinucleotide/biosynthesis/metabolism ; HeLa Cells ; Humans ; Isoenzymes/chemistry/metabolism ; Membrane Glycoproteins/metabolism ; Mice ; NADH, NADPH Oxidoreductases/metabolism ; NADPH Oxidase/chemistry/*metabolism ; Phosphotransferases (Alcohol Group Acceptor)/deficiency/genetics/*metabolism ; Protein Binding ; Protein Structure, Tertiary ; Reactive Oxygen Species/metabolism ; Receptors, Tumor Necrosis Factor, Type I/chemistry/*metabolism
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 14
    Publikationsdatum: 2009-03-06
    Beschreibung: Osmoregulated transporters sense intracellular osmotic pressure and respond to hyperosmotic stress by accumulation of osmolytes to restore normal hydration levels. Here we report the determination of the X-ray structure of a member of the family of betaine/choline/carnitine transporters, the Na(+)-coupled symporter BetP from Corynebacterium glutamicum, which is a highly effective osmoregulated uptake system for glycine betaine. Glycine betaine is bound in a tryptophan box occluded from both sides of the membrane with aromatic side chains lining the transport pathway. BetP has the same overall fold as three unrelated Na(+)-coupled symporters. Whereas these are crystallized in either the outward-facing or the inward-facing conformation, the BetP structure reveals a unique intermediate conformation in the Na(+)-coupled transport cycle. The trimeric architecture of BetP and the break in three-fold symmetry by the osmosensing C-terminal helices suggest a regulatory mechanism of Na(+)-coupled osmolyte transport to counteract osmotic stress.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ressl, Susanne -- Terwisscha van Scheltinga, Anke C -- Vonrhein, Clemens -- Ott, Vera -- Ziegler, Christine -- England -- Nature. 2009 Mar 5;458(7234):47-52. doi: 10.1038/nature07819.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Max Planck Institute of Biophysics, Department of Structural Biology, 60438 Frankfurt am Main, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19262666" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Bacterial Proteins/*chemistry/genetics/*metabolism ; Betaine/*metabolism ; Binding Sites ; Carrier Proteins/*chemistry/genetics/*metabolism ; Corynebacterium glutamicum/*chemistry/genetics ; Crystallography, X-Ray ; Ion Transport ; Models, Molecular ; Protein Binding ; Protein Structure, Quaternary ; Protein Structure, Tertiary ; Sodium/*metabolism ; Structure-Activity Relationship
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    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 15
    Publikationsdatum: 2009-11-20
    Beschreibung: Extra-cytoplasmic polypeptides are usually synthesized as 'preproteins' carrying amino-terminal, cleavable signal peptides and secreted across membranes by translocases. The main bacterial translocase comprises the SecYEG protein-conducting channel and the peripheral ATPase motor SecA. Most proteins destined for the periplasm and beyond are exported post-translationally by SecA. Preprotein targeting to SecA is thought to involve signal peptides and chaperones like SecB. Here we show that signal peptides have a new role beyond targeting: they are essential allosteric activators of the translocase. On docking on their binding groove on SecA, signal peptides act in trans to drive three successive states: first, 'triggering' that drives the translocase to a lower activation energy state; second, 'trapping' that engages non-native preprotein mature domains docked with high affinity on the secretion apparatus; and third, 'secretion' during which trapped mature domains undergo several turnovers of translocation in segments. A significant contribution by mature domains renders signal peptides less critical in bacterial secretory protein targeting than currently assumed. Rather, it is their function as allosteric activators of the translocase that renders signal peptides essential for protein secretion. A role for signal peptides and targeting sequences as allosteric activators may be universal in protein translocases.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2823582/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2823582/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gouridis, Giorgos -- Karamanou, Spyridoula -- Gelis, Ioannis -- Kalodimos, Charalampos G -- Economou, Anastassios -- GM73854/GM/NIGMS NIH HHS/ -- R01 GM073854/GM/NIGMS NIH HHS/ -- R01 GM073854-03/GM/NIGMS NIH HHS/ -- England -- Nature. 2009 Nov 19;462(7271):363-7. doi: 10.1038/nature08559.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology-Hellas, Iraklio, Crete 71110, Greece.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19924216" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Adenosine Triphosphatases/metabolism ; Alkaline Phosphatase/metabolism ; Enzyme Activators/*metabolism ; Escherichia coli/*enzymology ; Escherichia coli Proteins/*metabolism ; Periplasmic Proteins/metabolism ; Protein Binding ; Protein Sorting Signals/*physiology ; Protein Transport
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 16
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-05-30
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gowans, James -- England -- Nature. 2009 May 28;459(7246):506. doi: 10.1038/459506c.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19478763" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Clinical Trials as Topic/history ; Correspondence as Topic/history ; Female ; Great Britain ; History, 20th Century ; Humans ; Periodicals as Topic/*history
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 17
    Publikationsdatum: 2009-11-27
    Beschreibung: Protein design provides a rigorous test of our knowledge about proteins and allows the creation of novel enzymes for biotechnological applications. Whereas progress has been made in designing proteins that mimic native proteins structurally, it is more difficult to design functional proteins. In comparison to recent successes in designing non-metalloproteins, it is even more challenging to rationally design metalloproteins that reproduce both the structure and function of native metalloenzymes. This is because protein metal-binding sites are much more varied than non-metal-containing sites, in terms of different metal ion oxidation states, preferred geometry and metal ion ligand donor sets. Because of their variability, it has been difficult to predict metal-binding site properties in silico, as many of the parameters, such as force fields, are ill-defined. Therefore, the successful design of a structural and functional metalloprotein would greatly advance the field of protein design and our understanding of enzymes. Here we report a successful, rational design of a structural and functional model of a metalloprotein, nitric oxide reductase (NOR), by introducing three histidines and one glutamate, predicted as ligands in the active site of NOR, into the distal pocket of myoglobin. A crystal structure of the designed protein confirms that the minimized computer model contains a haem/non-haem Fe(B) centre that is remarkably similar to that in the crystal structure. This designed protein also exhibits NO reduction activity, and so models both the structure and function of NOR, offering insight that the active site glutamate is required for both iron binding and activity. These results show that structural and functional metalloproteins can be rationally designed in silico.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4297211/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4297211/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yeung, Natasha -- Lin, Ying-Wu -- Gao, Yi-Gui -- Zhao, Xuan -- Russell, Brandy S -- Lei, Lanyu -- Miner, Kyle D -- Robinson, Howard -- Lu, Yi -- GM062211/GM/NIGMS NIH HHS/ -- R01 GM062211/GM/NIGMS NIH HHS/ -- England -- Nature. 2009 Dec 24;462(7276):1079-82. doi: 10.1038/nature08620. Epub 2009 Nov 25.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19940850" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Crystallization ; Iron/metabolism ; Models, Molecular ; Myoglobin/chemistry ; Nitric Oxide/metabolism ; Oxidoreductases/*chemical synthesis/*chemistry/metabolism ; Protein Binding ; Protein Structure, Tertiary
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 18
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-03-28
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉England -- Nature. 2009 Mar 26;458(7237):385. doi: 10.1038/458386a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19325578" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Europe ; *Genes ; *Genetic Testing/economics/legislation & jurisprudence ; History, 20th Century ; History, 21st Century ; Humans ; Patents as Topic/history/*legislation & jurisprudence/*statistics & numerical ; data ; United States
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 19
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-04-17
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kell, Douglas -- England -- Nature. 2009 Apr 16;458(7240):831. doi: 10.1038/458831b.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19370012" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): History, 20th Century ; History, 21st Century ; Microbiological Techniques/*history ; Microbiology/*history
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 20
    Publikationsdatum: 2009-07-10
    Beschreibung: The modern Arctic Ocean is regarded as a barometer of global change and amplifier of global warming and therefore records of past Arctic change are critical for palaeoclimate reconstruction. Little is known of the state of the Arctic Ocean in the greenhouse period of the Late Cretaceous epoch (65-99 million years ago), yet records from such times may yield important clues to Arctic Ocean behaviour in near-future warmer climates. Here we present a seasonally resolved Cretaceous sedimentary record from the Alpha ridge of the Arctic Ocean. This palaeo-sediment trap provides new insight into the workings of the Cretaceous marine biological carbon pump. Seasonal primary production was dominated by diatom algae but was not related to upwelling as was previously hypothesized. Rather, production occurred within a stratified water column, involving specially adapted species in blooms resembling those of the modern North Pacific subtropical gyre, or those indicated for the Mediterranean sapropels. With increased CO(2) levels and warming currently driving increased stratification in the global ocean, this style of production that is adapted to stratification may become more widespread. Our evidence for seasonal diatom production and flux testify to an ice-free summer, but thin accumulations of terrigenous sediment within the diatom ooze are consistent with the presence of intermittent sea ice in the winter, supporting a wide body of evidence for low temperatures in the Late Cretaceous Arctic Ocean, rather than recent suggestions of a 15 degrees C mean annual temperature at this time.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Davies, Andrew -- Kemp, Alan E S -- Pike, Jennifer -- England -- Nature. 2009 Jul 9;460(7252):254-8. doi: 10.1038/nature08141.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉National Oceanography Centre Southampton, School of Ocean and Earth Science, University of Southampton, Southampton, SO14 3ZH, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19587768" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Arctic Regions ; Carbon Dioxide/metabolism ; Diatoms/metabolism ; Fossils ; Geologic Sediments/analysis/microbiology ; *Greenhouse Effect ; History, Ancient ; Ice Cover/chemistry ; Marine Biology ; Oceans and Seas ; *Seasons ; *Seawater ; *Temperature
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 21
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-05-16
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Davies, David -- England -- Nature. 2009 May 14;459(7244):163. doi: 10.1038/459163a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19444189" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): *Editorial Policies ; History, 20th Century ; Periodicals as Topic/*history
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 22
    Publikationsdatum: 2009-11-10
    Beschreibung: Abscisic acid (ABA) is a ubiquitous hormone that regulates plant growth, development and responses to environmental stresses. Its action is mediated by the PYR/PYL/RCAR family of START proteins, but it remains unclear how these receptors bind ABA and, in turn, how hormone binding leads to inhibition of the downstream type 2C protein phosphatase (PP2C) effectors. Here we report crystal structures of apo and ABA-bound receptors as well as a ternary PYL2-ABA-PP2C complex. The apo receptors contain an open ligand-binding pocket flanked by a gate that closes in response to ABA by way of conformational changes in two highly conserved beta-loops that serve as a gate and latch. Moreover, ABA-induced closure of the gate creates a surface that enables the receptor to dock into and competitively inhibit the PP2C active site. A conserved tryptophan in the PP2C inserts directly between the gate and latch, which functions to further lock the receptor in a closed conformation. Together, our results identify a conserved gate-latch-lock mechanism underlying ABA signalling.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2810868/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2810868/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Melcher, Karsten -- Ng, Ley-Moy -- Zhou, X Edward -- Soon, Fen-Fen -- Xu, Yong -- Suino-Powell, Kelly M -- Park, Sang-Youl -- Weiner, Joshua J -- Fujii, Hiroaki -- Chinnusamy, Viswanathan -- Kovach, Amanda -- Li, Jun -- Wang, Yonghong -- Li, Jiayang -- Peterson, Francis C -- Jensen, Davin R -- Yong, Eu-Leong -- Volkman, Brian F -- Cutler, Sean R -- Zhu, Jian-Kang -- Xu, H Eric -- R01 DK066202/DK/NIDDK NIH HHS/ -- R01 DK066202-04/DK/NIDDK NIH HHS/ -- R01 DK071662/DK/NIDDK NIH HHS/ -- R01 DK071662-05/DK/NIDDK NIH HHS/ -- R01 GM087413/GM/NIGMS NIH HHS/ -- R01 GM087413-01/GM/NIGMS NIH HHS/ -- R01 HL089301/HL/NHLBI NIH HHS/ -- R01 HL089301-03/HL/NHLBI NIH HHS/ -- England -- Nature. 2009 Dec 3;462(7273):602-8. doi: 10.1038/nature08613.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Structural Sciences, Van Andel Research Institute, 333 Bostwick Avenue, N.E., Grand Rapids, Michigan 49503, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19898420" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Abscisic Acid/*metabolism ; Arabidopsis/genetics/metabolism/*physiology ; Arabidopsis Proteins/*chemistry/genetics/metabolism/*physiology ; Binding Sites ; DNA Mutational Analysis ; *Models, Molecular ; Plants, Genetically Modified ; Protein Binding ; Protein Structure, Tertiary ; Signal Transduction/*physiology
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 23
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-05-16
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mellars, Paul -- England -- Nature. 2009 May 14;459(7244):176-7. doi: 10.1038/459176a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19444200" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; *Archaeology ; Female ; Germany ; History, Ancient ; Horns/chemistry ; Humans ; Sculpture/*history ; Sex Characteristics ; Symbolism
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 24
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-04-28
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gratzer, Walter -- England -- Nature. 2009 Apr 23;458(7241):983-4. doi: 10.1038/458983a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19396135" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): *Editorial Policies ; Great Britain ; History, 20th Century ; Periodicals as Topic/*history ; Physics/history ; Science/*history
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 25
    Publikationsdatum: 2009-04-28
    Beschreibung: Plant photosynthesis tends to increase with irradiance. However, recent theoretical and observational studies have demonstrated that photosynthesis is also more efficient under diffuse light conditions. Changes in cloud cover or atmospheric aerosol loadings, arising from either volcanic or anthropogenic emissions, alter both the total photosynthetically active radiation reaching the surface and the fraction of this radiation that is diffuse, with uncertain overall effects on global plant productivity and the land carbon sink. Here we estimate the impact of variations in diffuse fraction on the land carbon sink using a global model modified to account for the effects of variations in both direct and diffuse radiation on canopy photosynthesis. We estimate that variations in diffuse fraction, associated largely with the 'global dimming' period, enhanced the land carbon sink by approximately one-quarter between 1960 and 1999. However, under a climate mitigation scenario for the twenty-first century in which sulphate aerosols decline before atmospheric CO(2) is stabilized, this 'diffuse-radiation' fertilization effect declines rapidly to near zero by the end of the twenty-first century.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mercado, Lina M -- Bellouin, Nicolas -- Sitch, Stephen -- Boucher, Olivier -- Huntingford, Chris -- Wild, Martin -- Cox, Peter M -- England -- Nature. 2009 Apr 23;458(7241):1014-7. doi: 10.1038/nature07949.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Centre for Ecology and Hydrology, Wallingford OX10 8BB, UK. lmme@ceh.ac.uk〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19396143" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Aerosols/analysis/chemistry ; Atmosphere/*chemistry ; Carbon/*metabolism ; Carbon Dioxide/analysis ; *Darkness ; *Ecosystem ; Greenhouse Effect ; History, 20th Century ; History, 21st Century ; Photosynthesis/*radiation effects ; Plants/metabolism/*radiation effects ; Sulfates/metabolism ; *Sunlight ; Volcanic Eruptions
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 26
    Publikationsdatum: 2009-09-29
    Beschreibung: Activation of Janus kinase 2 (JAK2) by chromosomal translocations or point mutations is a frequent event in haematological malignancies. JAK2 is a non-receptor tyrosine kinase that regulates several cellular processes by inducing cytoplasmic signalling cascades. Here we show that human JAK2 is present in the nucleus of haematopoietic cells and directly phosphorylates Tyr 41 (Y41) on histone H3. Heterochromatin protein 1alpha (HP1alpha), but not HP1beta, specifically binds to this region of H3 through its chromo-shadow domain. Phosphorylation of H3Y41 by JAK2 prevents this binding. Inhibition of JAK2 activity in human leukaemic cells decreases both the expression of the haematopoietic oncogene lmo2 and the phosphorylation of H3Y41 at its promoter, while simultaneously increasing the binding of HP1alpha at the same site. Tauhese results identify a previously unrecognized nuclear role for JAK2 in the phosphorylation of H3Y41 and reveal a direct mechanistic link between two genes, jak2 and lmo2, involved in normal haematopoiesis and leukaemia.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3785147/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3785147/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dawson, Mark A -- Bannister, Andrew J -- Gottgens, Berthold -- Foster, Samuel D -- Bartke, Till -- Green, Anthony R -- Kouzarides, Tony -- 089957/Wellcome Trust/United Kingdom -- 12765/Cancer Research UK/United Kingdom -- G0800784/Medical Research Council/United Kingdom -- MC_UP_1102/2/Medical Research Council/United Kingdom -- Cancer Research UK/United Kingdom -- Medical Research Council/United Kingdom -- Wellcome Trust/United Kingdom -- England -- Nature. 2009 Oct 8;461(7265):819-22. doi: 10.1038/nature08448. Epub 2009 Sep 27.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cambridge Institute for Medical Research and Department of Haematology, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19783980" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Adaptor Proteins, Signal Transducing ; Animals ; Binding Sites ; Cell Line ; Cell Nucleus/enzymology ; Chromatin/chemistry/*metabolism ; Chromosomal Proteins, Non-Histone/*metabolism ; DNA-Binding Proteins/genetics ; Gene Expression Regulation, Neoplastic ; Hematopoiesis/genetics ; Hematopoietic Stem Cells/cytology/enzymology ; Histones/chemistry/genetics/*metabolism ; Humans ; Janus Kinase 2/antagonists & inhibitors/*metabolism ; LIM Domain Proteins ; Leukemia/enzymology/genetics/metabolism/pathology ; Metalloproteins/genetics ; Mice ; Oncogenes/genetics ; Phosphorylation ; Promoter Regions, Genetic/genetics ; Protein Binding ; Proto-Oncogene Proteins ; Signal Transduction ; Tyrosine/metabolism
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 27
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-11-20
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Buchenau, Jurgen -- England -- Nature. 2009 Nov 19;462(7271):284-5. doi: 10.1038/462284a.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of History at the University of North Carolina at Charlotte, 9201 University City Boulevard, Charlotte, North Carolina 28223, USA. jbuchenau@uncc.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19924194" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): *Biological Evolution ; Emigration and Immigration ; Europe ; History, 19th Century ; History, 20th Century ; Humans ; Latin America ; Public Policy/history/*trends
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 28
    Publikationsdatum: 2009-04-17
    Beschreibung: Interaction specificity is a required feature of biological networks and a necessary characteristic of protein or small-molecule reagents and therapeutics. The ability to alter or inhibit protein interactions selectively would advance basic and applied molecular science. Assessing or modelling interaction specificity requires treating multiple competing complexes, which presents computational and experimental challenges. Here we present a computational framework for designing protein-interaction specificity and use it to identify specific peptide partners for human basic-region leucine zipper (bZIP) transcription factors. Protein microarrays were used to characterize designed, synthetic ligands for all but one of 20 bZIP families. The bZIP proteins share strong sequence and structural similarities and thus are challenging targets to bind specifically. Nevertheless, many of the designs, including examples that bind the oncoproteins c-Jun, c-Fos and c-Maf (also called JUN, FOS and MAF, respectively), were selective for their targets over all 19 other families. Collectively, the designs exhibit a wide range of interaction profiles and demonstrate that human bZIPs have only sparsely sampled the possible interaction space accessible to them. Our computational method provides a way to systematically analyse trade-offs between stability and specificity and is suitable for use with many types of structure-scoring functions; thus, it may prove broadly useful as a tool for protein design.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2748673/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2748673/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Grigoryan, Gevorg -- Reinke, Aaron W -- Keating, Amy E -- GM67681/GM/NIGMS NIH HHS/ -- R01 GM067681/GM/NIGMS NIH HHS/ -- R01 GM067681-04/GM/NIGMS NIH HHS/ -- R01 GM067681-05/GM/NIGMS NIH HHS/ -- England -- Nature. 2009 Apr 16;458(7240):859-64. doi: 10.1038/nature07885.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉MIT Department of Biology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19370028" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Amino Acid Motifs ; Basic-Leucine Zipper Transcription Factors/*chemistry/classification/*metabolism ; Computational Biology/*methods ; Drug Design ; Humans ; Leucine Zippers ; Protein Array Analysis ; Protein Binding ; Protein Engineering/*methods ; Reproducibility of Results ; Substrate Specificity
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 29
    Publikationsdatum: 2009-05-05
    Beschreibung: The proteasome is a protease that controls diverse processes in eukaryotic cells. Its regulatory particle (RP) initiates the degradation of ubiquitin-protein conjugates by unfolding the substrate and translocating it into the proteasome core particle (CP) to be degraded. The RP has 19 subunits, and their pathway of assembly is not understood. Here we show that in the yeast Saccharomyces cerevisiae three proteins are found associated with RP but not with the RP-CP holoenzyme: Nas6, Rpn14 and Hsm3. Mutations in the corresponding genes confer proteasome loss-of-function phenotypes, despite their virtual absence from the holoenzyme. These effects result from deficient RP assembly. Thus, Nas6, Rpn14 and Hsm3 are RP chaperones. The RP contains six ATPases-the Rpt proteins-and each RP chaperone binds to the carboxy-terminal domain of a specific Rpt. We show in an accompanying study that RP assembly is templated through the Rpt C termini, apparently by their insertion into binding pockets in the CP. Thus, RP chaperones may regulate proteasome assembly by directly restricting the accessibility of Rpt C termini to the CP. In addition, competition between the RP chaperones and the CP for Rpt engagement may explain the release of RP chaperones as proteasomes mature.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727592/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727592/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Roelofs, Jeroen -- Park, Soyeon -- Haas, Wilhelm -- Tian, Geng -- McAllister, Fiona E -- Huo, Ying -- Lee, Byung-Hoon -- Zhang, Fan -- Shi, Yigong -- Gygi, Steven P -- Finley, Daniel -- 5F32GM75737-2/GM/NIGMS NIH HHS/ -- GM043601/GM/NIGMS NIH HHS/ -- GM67945/GM/NIGMS NIH HHS/ -- R37 GM043601/GM/NIGMS NIH HHS/ -- R37 GM043601-19/GM/NIGMS NIH HHS/ -- England -- Nature. 2009 Jun 11;459(7248):861-5. doi: 10.1038/nature08063.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19412159" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Adenosine Triphosphatases/chemistry/metabolism ; Carrier Proteins/genetics/metabolism ; Conserved Sequence ; Evolution, Molecular ; Holoenzymes/chemistry/metabolism ; Humans ; Models, Molecular ; Molecular Chaperones/genetics/*metabolism ; Mutation ; Phenotype ; Proteasome Endopeptidase Complex/*chemistry/genetics/*metabolism ; Protein Binding ; Protein Structure, Tertiary ; Proto-Oncogene Proteins/genetics/metabolism ; Saccharomyces cerevisiae/*enzymology/genetics ; Saccharomyces cerevisiae Proteins/genetics/metabolism
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 30
    Publikationsdatum: 2009-09-26
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rockstrom, Johan -- Steffen, Will -- Noone, Kevin -- Persson, Asa -- Chapin, F Stuart 3rd -- Lambin, Eric F -- Lenton, Timothy M -- Scheffer, Marten -- Folke, Carl -- Schellnhuber, Hans Joachim -- Nykvist, Bjorn -- de Wit, Cynthia A -- Hughes, Terry -- van der Leeuw, Sander -- Rodhe, Henning -- Sorlin, Sverker -- Snyder, Peter K -- Costanza, Robert -- Svedin, Uno -- Falkenmark, Malin -- Karlberg, Louise -- Corell, Robert W -- Fabry, Victoria J -- Hansen, James -- Walker, Brian -- Liverman, Diana -- Richardson, Katherine -- Crutzen, Paul -- Foley, Jonathan A -- England -- Nature. 2009 Sep 24;461(7263):472-5. doi: 10.1038/461472a.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Stockholm Resilience Centre, Stockholm University, Kraftriket 2B, 10691 Stockholm, Sweden.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19779433" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Biodiversity ; Civilization ; Conservation of Natural Resources/*methods/trends ; *Earth (Planet) ; Ecology/*methods/*trends ; *Ecosystem ; Extinction, Biological ; Fossils ; Green Chemistry Technology/*methods/trends ; Greenhouse Effect ; History, 20th Century ; History, 21st Century ; History, Ancient ; *Human Activities/history ; Humans ; Nitrogen/metabolism ; Phosphorus/metabolism
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 31
    Publikationsdatum: 2009-06-03
    Beschreibung: The rapid turnover and exfoliation of mucosal epithelial cells provides an innate defence system against bacterial infection. Nevertheless, many pathogenic bacteria, including Shigella, are able to surmount exfoliation and colonize the epithelium efficiently. Here we show that the Shigella flexneri effector OspE (consisting of OspE1 and OspE2 proteins), which is highly conserved among enteropathogenic Escherichia coli, enterohaemorrhagic E. coli, Citrobacter rodentium and Salmonella strains, reinforces host cell adherence to the basement membrane by interacting with integrin-linked kinase (ILK). The number of focal adhesions was augmented along with membrane fraction ILK by ILK-OspE binding. The interaction between ILK and OspE increased cell surface levels of 1 integrin and suppressed phosphorylation of focal adhesion kinase and paxillin, which are required for rapid turnover of focal adhesion in cell motility. Nocodazole-washout-induced focal adhesion disassembly was blocked by expression of OspE. Polarized epithelial cells infected with a Shigella mutant lacking the ospE gene underwent more rapid cell detachment than cells infected with wild-type Shigella. Infection of guinea pig colons with Shigella corroborated the pivotal role of the OspE-ILK interaction in suppressing epithelial detachment, increasing bacterial cell-to-cell spreading, and promoting bacterial colonization. These results indicate that Shigella sustain their infectious foothold by using special tactics to prevent detachment of infected cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kim, Minsoo -- Ogawa, Michinaga -- Fujita, Yukihiro -- Yoshikawa, Yuko -- Nagai, Takeshi -- Koyama, Tomohiro -- Nagai, Shinya -- Lange, Anika -- Fassler, Reinhard -- Sasakawa, Chihiro -- England -- Nature. 2009 May 28;459(7246):578-82. doi: 10.1038/nature07952.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Infectious Disease Control, International Research Center for Infectious Diseases, University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19489119" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Antigens, CD29/metabolism ; Bacterial Outer Membrane Proteins/genetics/metabolism ; Cell Adhesion/drug effects/*physiology ; Cell Polarity ; Colon/microbiology ; Epithelial Cells/cytology/microbiology ; Focal Adhesions/drug effects/*physiology ; Guinea Pigs ; HeLa Cells ; Humans ; Mice ; Nocodazole/pharmacology ; Phosphorylation ; Protein Binding ; Protein-Serine-Threonine Kinases/*metabolism ; Shigella flexneri/pathogenicity/*physiology ; Virulence Factors/deficiency/genetics/metabolism
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 32
    Publikationsdatum: 2009-06-19
    Beschreibung: Several hundred malaria parasite proteins are exported beyond an encasing vacuole and into the cytosol of the host erythrocyte, a process that is central to the virulence and viability of the causative Plasmodium species. The trafficking machinery responsible for this export is unknown. Here we identify in Plasmodium falciparum a translocon of exported proteins (PTEX), which is located in the vacuole membrane. The PTEX complex is ATP-powered, and comprises heat shock protein 101 (HSP101; a ClpA/B-like ATPase from the AAA+ superfamily, of a type commonly associated with protein translocons), a novel protein termed PTEX150 and a known parasite protein, exported protein 2 (EXP2). EXP2 is the potential channel, as it is the membrane-associated component of the core PTEX complex. Two other proteins, a new protein PTEX88 and thioredoxin 2 (TRX2), were also identified as PTEX components. As a common portal for numerous crucial processes, this translocon offers a new avenue for therapeutic intervention.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2725363/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2725363/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉de Koning-Ward, Tania F -- Gilson, Paul R -- Boddey, Justin A -- Rug, Melanie -- Smith, Brian J -- Papenfuss, Anthony T -- Sanders, Paul R -- Lundie, Rachel J -- Maier, Alexander G -- Cowman, Alan F -- Crabb, Brendan S -- R01 AI044008-11/AI/NIAID NIH HHS/ -- R01 AI44008/AI/NIAID NIH HHS/ -- Howard Hughes Medical Institute/ -- England -- Nature. 2009 Jun 18;459(7249):945-9. doi: 10.1038/nature08104.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉The Walter & Eliza Hall Institute of Medical Research, Melbourne 3052, Australia.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19536257" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Animals, Genetically Modified ; Malaria, Falciparum/*parasitology ; Models, Biological ; Multiprotein Complexes/*chemistry/*metabolism ; Plasmodium falciparum/*metabolism ; Protein Binding ; Protein Transport ; Protozoan Proteins/*metabolism
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 33
    Publikationsdatum: 2009-10-30
    Beschreibung: The recognition of specific DNA sequences by proteins is thought to depend on two types of mechanism: one that involves the formation of hydrogen bonds with specific bases, primarily in the major groove, and one involving sequence-dependent deformations of the DNA helix. By comprehensively analysing the three-dimensional structures of protein-DNA complexes, here we show that the binding of arginine residues to narrow minor grooves is a widely used mode for protein-DNA recognition. This readout mechanism exploits the phenomenon that narrow minor grooves strongly enhance the negative electrostatic potential of the DNA. The nucleosome core particle offers a prominent example of this effect. Minor-groove narrowing is often associated with the presence of A-tracts, AT-rich sequences that exclude the flexible TpA step. These findings indicate that the ability to detect local variations in DNA shape and electrostatic potential is a general mechanism that enables proteins to use information in the minor groove, which otherwise offers few opportunities for the formation of base-specific hydrogen bonds, to achieve DNA-binding specificity.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2793086/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2793086/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rohs, Remo -- West, Sean M -- Sosinsky, Alona -- Liu, Peng -- Mann, Richard S -- Honig, Barry -- GM54510/GM/NIGMS NIH HHS/ -- R01 GM030518/GM/NIGMS NIH HHS/ -- U54 CA121852/CA/NCI NIH HHS/ -- U54 CA121852-05/CA/NCI NIH HHS/ -- Howard Hughes Medical Institute/ -- England -- Nature. 2009 Oct 29;461(7268):1248-53. doi: 10.1038/nature08473.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Center for Computational Biology and Bioinformatics, Department of Biochemistry and Molecular Biophysics, Columbia University, 1130 Saint Nicholas Avenue, New York, New York 10032, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19865164" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): AT Rich Sequence/genetics ; Animals ; Arginine/metabolism ; Base Sequence ; DNA/*chemistry/genetics/*metabolism ; DNA-Binding Proteins/chemistry/*metabolism ; Databases, Factual ; Hydrogen Bonding ; Lysine ; *Nucleic Acid Conformation ; Nucleosomes/chemistry/metabolism ; Protein Binding ; Saccharomyces cerevisiae ; Static Electricity
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 34
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-09-17
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zahn, Rainer -- England -- Nature. 2009 Jul 16;460(7253):335-6. doi: 10.1038/460335a.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institucio Catalana de Recerca i Estudis Avancats (ICREA), Institut de Ciencia i Tecnologia Ambientals and Departmento de Geologia, Universitat Autonoma de Barcelona, Bellaterra E-08193, Spain. rainer.zahn@uab.cat.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19606136" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Antarctic Regions ; Atlantic Ocean ; Atmosphere/chemistry ; Carbon Dioxide/metabolism ; *Climate ; Geologic Sediments/microbiology ; History, Ancient ; Ice Cover ; Indian Ocean ; Seawater/chemistry ; *Temperature ; *Water Movements
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 35
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-01-30
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Burnap, Don -- England -- Nature. 2009 Jan 29;457(7229):533. doi: 10.1038/457533b.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19177110" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): *Biomedical Enhancement/history ; Cannabinoids/*history ; *Cognition ; Creativity ; Hallucinogens/*history ; Health ; History, 20th Century ; History, 21st Century ; Humans ; Lysergic Acid Diethylamide/*history
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 36
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    Unbekannt
    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-02-27
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Demain, Arnold L -- England -- Nature. 2009 Feb 26;457(7233):1079. doi: 10.1038/4571079c.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19242451" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Biotechnology/*history ; Congresses as Topic/history ; Cuba ; History, 20th Century ; *International Cooperation ; Politics ; Research Personnel/*history ; United States
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 37
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-03-06
    Beschreibung: 〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440453/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440453/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Burz, David S -- Shekhtman, Alexander -- R01 GM085006/GM/NIGMS NIH HHS/ -- England -- Nature. 2009 Mar 5;458(7234):37-8. doi: 10.1038/458037a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19262658" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Bacterial Proteins/chemistry/genetics ; Drug Evaluation, Preclinical/methods ; Escherichia coli/*cytology/genetics/*metabolism ; Humans ; Intracellular Space/*metabolism ; Nuclear Magnetic Resonance, Biomolecular/*methods ; Oocytes/metabolism ; Protein Binding ; Thermus thermophilus/genetics ; Xenopus laevis
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 38
    Publikationsdatum: 2009-10-27
    Beschreibung: The phytohormone abscisic acid (ABA) mediates the adaptation of plants to environmental stresses such as drought and regulates developmental signals such as seed maturation. Within plants, the PYR/PYL/RCAR family of START proteins receives ABA to inhibit the phosphatase activity of the group-A protein phosphatases 2C (PP2Cs), which are major negative regulators in ABA signalling. Here we present the crystal structures of the ABA receptor PYL1 bound with (+)-ABA, and the complex formed by the further binding of (+)-ABA-bound PYL1 with the PP2C protein ABI1. PYL1 binds (+)-ABA using the START-protein-specific ligand-binding site, thereby forming a hydrophobic pocket on the surface of the closed lid. (+)-ABA-bound PYL1 tightly interacts with a PP2C domain of ABI1 by using the hydrophobic pocket to cover the active site of ABI1 like a plug. Our results reveal the structural basis of the mechanism of (+)-ABA-dependent inhibition of ABI1 by PYL1 in ABA signalling.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Miyazono, Ken-Ichi -- Miyakawa, Takuya -- Sawano, Yoriko -- Kubota, Keiko -- Kang, Hee-Jin -- Asano, Atsuko -- Miyauchi, Yumiko -- Takahashi, Mihoko -- Zhi, Yuehua -- Fujita, Yasunari -- Yoshida, Takuya -- Kodaira, Ken-Suke -- Yamaguchi-Shinozaki, Kazuko -- Tanokura, Masaru -- England -- Nature. 2009 Dec 3;462(7273):609-14. doi: 10.1038/nature08583.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19855379" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Abscisic Acid/*physiology ; Arabidopsis/*physiology ; Arabidopsis Proteins/*chemistry/*metabolism ; Binding Sites ; *Models, Molecular ; Protein Binding ; Protein Structure, Tertiary ; Recombinant Proteins/chemistry/metabolism ; *Signal Transduction
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 39
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-05-22
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rothschild, Lynn J -- England -- Nature. 2009 May 21;459(7245):335-6. doi: 10.1038/459335a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19458704" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Biological Evolution ; *Earth (Planet) ; History, Ancient ; Hot Temperature ; *Meteoroids ; *Models, Biological ; Moon ; *Origin of Life ; Silicates/analysis ; Sterilization ; Time Factors ; Zirconium/analysis
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 40
    Publikationsdatum: 2009-10-30
    Beschreibung: Enzymes use substrate-binding energy both to promote ground-state association and to stabilize the reaction transition state selectively. The monomeric homing endonuclease I-AniI cleaves with high sequence specificity in the centre of a 20-base-pair (bp) DNA target site, with the amino (N)-terminal domain of the enzyme making extensive binding interactions with the left (-) side of the target site and the similarly structured carboxy (C)-terminal domain interacting with the right (+) side. Here we show that, despite the approximate twofold symmetry of the enzyme-DNA complex, there is almost complete segregation of interactions responsible for substrate binding to the (-) side of the interface and interactions responsible for transition-state stabilization to the (+) side. Although single base-pair substitutions throughout the entire DNA target site reduce catalytic efficiency, mutations in the (-) DNA half-site almost exclusively increase the dissociation constant (K(D)) and the Michaelis constant under single-turnover conditions (K(M)*), and those in the (+) half-site primarily decrease the turnover number (k(cat)*). The reduction of activity produced by mutations on the (-) side, but not mutations on the (+) side, can be suppressed by tethering the substrate to the endonuclease displayed on the surface of yeast. This dramatic asymmetry in the use of enzyme-substrate binding energy for catalysis has direct relevance to the redesign of endonucleases to cleave genomic target sites for gene therapy and other applications. Computationally redesigned enzymes that achieve new specificities on the (-) side do so by modulating K(M)*, whereas redesigns with altered specificities on the (+) side modulate k(cat)*. Our results illustrate how classical enzymology and modern protein design can each inform the other.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2771326/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2771326/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Thyme, Summer B -- Jarjour, Jordan -- Takeuchi, Ryo -- Havranek, James J -- Ashworth, Justin -- Scharenberg, Andrew M -- Stoddard, Barry L -- Baker, David -- GM084433/GM/NIGMS NIH HHS/ -- R00 RR024107/RR/NCRR NIH HHS/ -- R00 RR024107-03/RR/NCRR NIH HHS/ -- R00 RR024107-04/RR/NCRR NIH HHS/ -- RL1 GM084433/GM/NIGMS NIH HHS/ -- RL1 GM084433-03/GM/NIGMS NIH HHS/ -- RL1CA133832/CA/NCI NIH HHS/ -- Howard Hughes Medical Institute/ -- England -- Nature. 2009 Oct 29;461(7268):1300-4. doi: 10.1038/nature08508.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA. sthyme@u.washington.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19865174" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Binding Sites ; *Biocatalysis ; Computational Biology ; *Computer Simulation ; DNA/chemistry/metabolism ; Endonucleases/chemistry/*metabolism ; Kinetics ; Models, Molecular ; Protein Binding ; Protein Conformation ; RNA-Directed DNA Polymerase/chemistry/*metabolism ; Saccharomyces cerevisiae/metabolism ; Substrate Specificity ; *Thermodynamics
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 41
    Publikationsdatum: 2009-02-06
    Beschreibung: The Atlantic meridional overturning circulation (AMOC) transports warm salty surface waters to high latitudes, where they cool, sink and return southwards at depth. Through its attendant meridional heat transport, the AMOC helps maintain a warm northwestern European climate, and acts as a control on the global climate. Past climate fluctuations during the Holocene epoch ( approximately 11,700 years ago to the present) have been linked with changes in North Atlantic Ocean circulation. The behaviour of the surface flowing salty water that helped drive overturning during past climatic changes is, however, not well known. Here we investigate the temperature and salinity changes of a substantial surface inflow to a region of deep-water formation throughout the Holocene. We find that the inflow has undergone millennial-scale variations in temperature and salinity ( approximately 3.5 degrees C and approximately 1.5 practical salinity units, respectively) most probably controlled by subpolar gyre dynamics. The temperature and salinity variations correlate with previously reported periods of rapid climate change. The inflow becomes more saline during enhanced freshwater flux to the subpolar North Atlantic. Model studies predict a weakening of AMOC in response to enhanced Arctic freshwater fluxes, although the inflow can compensate on decadal timescales by becoming more saline. Our data suggest that such a negative feedback mechanism may have operated during past intervals of climate change.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Thornalley, David J R -- Elderfield, Harry -- McCave, I Nick -- England -- Nature. 2009 Feb 5;457(7230):711-4. doi: 10.1038/nature07717.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉The Godwin Laboratory for Palaeoclimate Research, Department of Earth Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EQ, UK. d.thornalley@cantab.net〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19194447" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Atlantic Ocean ; Calcium Carbonate/analysis ; Climate ; Feedback ; Fresh Water/analysis/chemistry ; History, Ancient ; Oxygen Isotopes ; Plankton/metabolism ; *Salinity ; Seawater/*chemistry ; *Temperature ; *Water Movements
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 42
    facet.materialart.
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-07-10
    Beschreibung: Many aspects of the carbon cycle can be assessed from temporal changes in the (13)C/(12)C ratio of oceanic bicarbonate. (13)C/(12)C can temporarily rise when large amounts of (13)C-depleted photosynthetic organic matter are buried at enhanced rates, and can decrease if phytomass is rapidly oxidized or if low (13)C is rapidly released from methane clathrates. Assuming that variations of the marine (13)C/(12)C ratio are directly recorded in carbonate rocks, thousands of carbon isotope analyses of late Precambrian examples have been published to correlate these otherwise undatable strata and to document perturbations to the carbon cycle just before the great expansion of metazoan life. Low (13)C/(12)C in some Neoproterozoic carbonates is considered evidence of carbon cycle perturbations unique to the Precambrian. These include complete oxidation of all organic matter in the ocean and complete productivity collapse such that low-(13)C/(12)C hydrothermal CO(2) becomes the main input of carbon. Here we compile all published oxygen and carbon isotope data for Neoproterozoic marine carbonates, and consider them in terms of processes known to alter the isotopic composition during transformation of the initial precipitate into limestone/dolostone. We show that the combined oxygen and carbon isotope systematics are identical to those of well-understood Phanerozoic examples that lithified in coastal pore fluids, receiving a large groundwater influx of photosynthetic carbon from terrestrial phytomass. Rather than being perturbations to the carbon cycle, widely reported decreases in (13)C/(12)C in Neoproterozoic carbonates are more easily interpreted in the same way as is done for Phanerozoic examples. This influx of terrestrial carbon is not apparent in carbonates older than approximately 850 Myr, so we infer an explosion of photosynthesizing communities on late Precambrian land surfaces. As a result, biotically enhanced weathering generated carbon-bearing soils on a large scale and their detrital sedimentation sequestered carbon. This facilitated a rise in O(2) necessary for the expansion of multicellular life.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Knauth, L Paul -- Kennedy, Martin J -- England -- Nature. 2009 Aug 6;460(7256):728-32. doi: 10.1038/nature08213. Epub 2009 Jul 8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉School of Earth and Space Exploration, Arizona State University, Tempe, Arizona 85287-1404, USA. Knauth@asu.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19587681" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Biomass ; Calcium Carbonate/chemistry ; Carbon/chemistry/*metabolism ; Carbon Isotopes ; Carbonates/*analysis/*chemistry ; Earth (Planet) ; Geologic Sediments/*chemistry ; History, Ancient ; Magnesium/chemistry ; Oceans and Seas ; Oxygen/analysis/metabolism ; Oxygen Isotopes ; *Photosynthesis ; Plants/metabolism
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  • 43
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-02-20
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zhdanov, Renad -- England -- Nature. 2009 Feb 19;457(7232):956. doi: 10.1038/457956a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19225497" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Europe ; History, 19th Century ; History, 20th Century ; History, 21st Century ; Research Personnel/*history ; Science/*history ; Turkey ; Universities/trends
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  • 44
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-09-19
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Monastersky, Richard -- England -- Nature. 2009 Jul 16;460(7253):314-5. doi: 10.1038/460314a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19606113" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Astronauts/economics/*history/*trends ; Europe ; History, 20th Century ; History, 21st Century ; *Moon ; Research Personnel/psychology ; Space Flight/economics/*history/*trends ; United States ; United States National Aeronautics and Space ; Administration/economics/*history/*trends
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  • 45
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-04-28
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gurney, Kevin Robert -- England -- Nature. 2009 Apr 23;458(7241):977-9. doi: 10.1038/458977a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19396132" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Atmosphere/chemistry ; Carbon/analysis/*metabolism ; Carbon Dioxide/analysis/chemistry/metabolism ; China ; *Ecosystem ; *Fossil Fuels ; History, 20th Century ; History, 21st Century ; Soil/analysis ; Trees/metabolism ; United States
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  • 46
    Publikationsdatum: 2009-03-20
    Beschreibung: Ornithischia is one of the two major groups of dinosaurs, with heterodontosauridae as one of its major clades. Heterodontosauridae is characterized by small, gracile bodies and a problematic phylogenetic position. Recent phylogenetic work indicates that it represents the most basal group of all well-known ornithischians. Previous heterodontosaurid records are mainly from the Early Jurassic period (205-190 million years ago) of Africa. Here we report a new heterodontosaurid, Tianyulong confuciusi gen. et sp. nov., from the Early Cretaceous period (144-99 million years ago) of western Liaoning Province, China. Tianyulong extends the geographical distribution of heterodontosaurids to Asia and confirms the clade's previously questionable temporal range extension into the Early Cretaceous period. More surprisingly, Tianyulong bears long, singular and unbranched filamentous integumentary (outer skin) structures. This represents the first confirmed report, to our knowledge, of filamentous integumentary structures in an ornithischian dinosaur.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zheng, Xiao-Ting -- You, Hai-Lu -- Xu, Xing -- Dong, Zhi-Ming -- England -- Nature. 2009 Mar 19;458(7236):333-6. doi: 10.1038/nature07856.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Shandong Tianyu Museum of Nature, Lianhuashan Road West, Pingyi, Shandong, 273300, China.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19295609" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; *Biological Evolution ; China ; Dentition ; Dinosaurs/*anatomy & histology/*classification ; Feathers/anatomy & histology ; Fossils ; History, Ancient ; Integumentary System/*anatomy & histology ; Phylogeny ; Skin/anatomy & histology ; Skull/anatomy & histology
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  • 47
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-11-06
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Todes, Daniel -- England -- Nature. 2009 Nov 5;462(7269):36-7. doi: 10.1038/462036a.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of the History of Medicine at Johns Hopkins University, 1900 East Monument Street, Baltimore, Maryland 21205, USA. dtodes@jhmi.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19890312" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; *Biological Evolution ; Biological Science Disciplines/*history ; *Competitive Behavior ; Cooperative Behavior ; *Cultural Diversity ; Food Supply ; Great Britain ; History, 19th Century ; History, 20th Century ; Humans ; Literature, Modern/history ; Metaphor ; Models, Biological ; Population Density ; Russia ; Selection, Genetic
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  • 48
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-01-09
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉England -- Nature. 2009 Jan 8;457(7226):129-30. doi: 10.1038/457129b.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19129800" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): *Conflict (Psychology) ; *Cultural Diversity ; History, 20th Century ; History, 21st Century ; Social Sciences/*history ; United States ; Violence ; Warfare
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  • 49
    Publikationsdatum: 2009-10-02
    Beschreibung: A key step in many chromatin-related processes is the recognition of histone post-translational modifications by effector modules such as bromodomains and chromo-like domains of the Royal family. Whereas effector-mediated recognition of single post-translational modifications is well characterized, how the cell achieves combinatorial readout of histones bearing multiple modifications is poorly understood. One mechanism involves multivalent binding by linked effector modules. For example, the tandem bromodomains of human TATA-binding protein-associated factor-1 (TAF1) bind better to a diacetylated histone H4 tail than to monoacetylated tails, a cooperative effect attributed to each bromodomain engaging one acetyl-lysine mark. Here we report a distinct mechanism of combinatorial readout for the mouse TAF1 homologue Brdt, a testis-specific member of the BET protein family. Brdt associates with hyperacetylated histone H4 (ref. 7) and is implicated in the marked chromatin remodelling that follows histone hyperacetylation during spermiogenesis, the stage of spermatogenesis in which post-meiotic germ cells mature into fully differentiated sperm. Notably, we find that a single bromodomain (BD1) of Brdt is responsible for selectively recognizing histone H4 tails bearing two or more acetylation marks. The crystal structure of BD1 bound to a diacetylated H4 tail shows how two acetyl-lysine residues cooperate to interact with one binding pocket. Structure-based mutagenesis that reduces the selectivity of BD1 towards diacetylated tails destabilizes the association of Brdt with acetylated chromatin in vivo. Structural analysis suggests that other chromatin-associated proteins may be capable of a similar mode of ligand recognition, including yeast Bdf1, human TAF1 and human CBP/p300 (also known as CREBBP and EP300, respectively). Our findings describe a new mechanism for the combinatorial readout of histone modifications in which a single effector module engages two marks on a histone tail as a composite binding epitope.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Moriniere, Jeanne -- Rousseaux, Sophie -- Steuerwald, Ulrich -- Soler-Lopez, Montserrat -- Curtet, Sandrine -- Vitte, Anne-Laure -- Govin, Jerome -- Gaucher, Jonathan -- Sadoul, Karin -- Hart, Darren J -- Krijgsveld, Jeroen -- Khochbin, Saadi -- Muller, Christoph W -- Petosa, Carlo -- England -- Nature. 2009 Oct 1;461(7264):664-8. doi: 10.1038/nature08397.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉European Molecular Biology Laboratory, Grenoble Outstation, 6 rue Jules Horowitz, BP 181, 38042 Grenoble Cedex 9, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19794495" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Acetylation ; Allosteric Regulation ; Animals ; Binding Sites ; COS Cells ; Cercopithecus aethiops ; Chromatin/chemistry/metabolism ; Crystallography, X-Ray ; Histones/*chemistry/*metabolism ; Lysine/metabolism ; Mice ; Models, Molecular ; Nuclear Proteins/*chemistry/genetics/*metabolism ; Protein Binding ; Protein Conformation ; Protein Structure, Tertiary ; Substrate Specificity
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  • 50
    Publikationsdatum: 2009-11-06
    Beschreibung: Development requires the establishment of precise patterns of gene expression, which are primarily controlled by transcription factors binding to cis-regulatory modules. Although transcription factor occupancy can now be identified at genome-wide scales, decoding this regulatory landscape remains a daunting challenge. Here we used a novel approach to predict spatio-temporal cis-regulatory activity based only on in vivo transcription factor binding and enhancer activity data. We generated a high-resolution atlas of cis-regulatory modules describing their temporal and combinatorial occupancy during Drosophila mesoderm development. The binding profiles of cis-regulatory modules with characterized expression were used to train support vector machines to predict five spatio-temporal expression patterns. In vivo transgenic reporter assays demonstrate the high accuracy of these predictions and reveal an unanticipated plasticity in transcription factor binding leading to similar expression. This data-driven approach does not require previous knowledge of transcription factor sequence affinity, function or expression, making it widely applicable.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zinzen, Robert P -- Girardot, Charles -- Gagneur, Julien -- Braun, Martina -- Furlong, Eileen E M -- England -- Nature. 2009 Nov 5;462(7269):65-70. doi: 10.1038/nature08531.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉European Molecular Biology Laboratory, D-69117 Heidelberg, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19890324" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Animals, Genetically Modified ; Artificial Intelligence ; Chromatin Immunoprecipitation ; Conserved Sequence/genetics ; Databases, Genetic ; Drosophila melanogaster/*embryology/*genetics ; Enhancer Elements, Genetic/genetics ; *Gene Expression Regulation, Developmental/genetics ; Genes, Reporter/genetics ; Mesoderm/embryology/metabolism ; *Models, Genetic ; Protein Binding ; Time Factors ; Transcription Factors/*metabolism
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  • 51
    Publikationsdatum: 2009-12-18
    Beschreibung: With polar temperatures approximately 3-5 degrees C warmer than today, the last interglacial stage (approximately 125 kyr ago) serves as a partial analogue for 1-2 degrees C global warming scenarios. Geological records from several sites indicate that local sea levels during the last interglacial were higher than today, but because local sea levels differ from global sea level, accurately reconstructing past global sea level requires an integrated analysis of globally distributed data sets. Here we present an extensive compilation of local sea level indicators and a statistical approach for estimating global sea level, local sea levels, ice sheet volumes and their associated uncertainties. We find a 95% probability that global sea level peaked at least 6.6 m higher than today during the last interglacial; it is likely (67% probability) to have exceeded 8.0 m but is unlikely (33% probability) to have exceeded 9.4 m. When global sea level was close to its current level (〉or=-10 m), the millennial average rate of global sea level rise is very likely to have exceeded 5.6 m kyr(-1) but is unlikely to have exceeded 9.2 m kyr(-1). Our analysis extends previous last interglacial sea level studies by integrating literature observations within a probabilistic framework that accounts for the physics of sea level change. The results highlight the long-term vulnerability of ice sheets to even relatively low levels of sustained global warming.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kopp, Robert E -- Simons, Frederik J -- Mitrovica, Jerry X -- Maloof, Adam C -- Oppenheimer, Michael -- England -- Nature. 2009 Dec 17;462(7275):863-7. doi: 10.1038/nature08686.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Geosciences, Princeton University, Princeton, New Jersey 08544, USA. rkopp@alumni.caltech.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20016591" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Algorithms ; Antarctic Regions ; Global Warming/*statistics & numerical data ; Greenhouse Effect ; Greenland ; History, 21st Century ; History, Ancient ; *Ice Cover ; Models, Theoretical ; Oceans and Seas ; *Probability ; Seawater/*analysis ; *Temperature ; Time Factors ; Uncertainty
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  • 52
    Publikationsdatum: 2009-10-02
    Beschreibung: The ASCE (additional strand, conserved E) superfamily of proteins consists of structurally similar ATPases associated with diverse cellular activities involving metabolism and transport of proteins and nucleic acids in all forms of life. A subset of these enzymes consists of multimeric ringed pumps responsible for DNA transport in processes including genome packaging in adenoviruses, herpesviruses, poxviruses and tailed bacteriophages. Although their mechanism of mechanochemical conversion is beginning to be understood, little is known about how these motors engage their nucleic acid substrates. Questions remain as to whether the motors contact a single DNA element, such as a phosphate or a base, or whether contacts are distributed over several parts of the DNA. Furthermore, the role of these contacts in the mechanochemical cycle is unknown. Here we use the genome packaging motor of the Bacillus subtilis bacteriophage varphi29 (ref. 4) to address these questions. The full mechanochemical cycle of the motor, in which the ATPase is a pentameric-ring of gene product 16 (gp16), involves two phases-an ATP-loading dwell followed by a translocation burst of four 2.5-base-pair (bp) steps triggered by hydrolysis product release. By challenging the motor with a variety of modified DNA substrates, we show that during the dwell phase important contacts are made with adjacent phosphates every 10-bp on the 5'-3' strand in the direction of packaging. As well as providing stable, long-lived contacts, these phosphate interactions also regulate the chemical cycle. In contrast, during the burst phase, we find that DNA translocation is driven against large forces by extensive contacts, some of which are not specific to the chemical moieties of DNA. Such promiscuous, nonspecific contacts may reflect common translocase-substrate interactions for both the nucleic acid and protein translocases of the ASCE superfamily.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2769991/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2769991/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Aathavan, K -- Politzer, Adam T -- Kaplan, Ariel -- Moffitt, Jeffrey R -- Chemla, Yann R -- Grimes, Shelley -- Jardine, Paul J -- Anderson, Dwight L -- Bustamante, Carlos -- DE-003606/DE/NIDCR NIH HHS/ -- GM-059604/GM/NIGMS NIH HHS/ -- GM-071552/GM/NIGMS NIH HHS/ -- R01 GM059604/GM/NIGMS NIH HHS/ -- R01 GM059604-09A1/GM/NIGMS NIH HHS/ -- R01 GM071552/GM/NIGMS NIH HHS/ -- R01 GM071552-04/GM/NIGMS NIH HHS/ -- Howard Hughes Medical Institute/ -- England -- Nature. 2009 Oct 1;461(7264):669-73. doi: 10.1038/nature08443.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Biophysics Graduate Group, University of California, Berkeley, California 94720, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19794496" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Adenosine Triphosphatases/chemistry/*metabolism ; Adenosine Triphosphate/metabolism ; Bacillus Phages/enzymology/genetics/*metabolism ; Bacillus subtilis/*virology ; Biological Transport ; DNA, Viral/chemistry/*metabolism ; DNA-Binding Proteins/chemistry/metabolism ; Genome, Viral ; Hydrolysis ; Molecular Motor Proteins/chemistry/*metabolism ; Phosphates/metabolism ; Protein Binding ; Substrate Specificity ; Viral Proteins/chemistry/*metabolism ; Virus Assembly/*physiology
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  • 53
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-12-18
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dolgin, Elie -- England -- Nature. 2009 Dec 17;462(7275):843-5. doi: 10.1038/462843a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20016572" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Female ; Genome, Human/*genetics ; History, 20th Century ; History, 21st Century ; *Human Genome Project/history ; Humans ; Male ; Reproducibility of Results ; Research Design ; *Research Personnel ; Research Subjects
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  • 54
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-12-25
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hand, Eric -- England -- Nature. 2009 Dec 24;462(7276):978-83. doi: 10.1038/462978a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20033016" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Conservation of Natural Resources ; *Global Warming ; History, 20th Century ; History, 21st Century ; Nobel Prize ; *Physics ; Policy Making ; United States
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  • 55
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-10-09
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Abbott, Alison -- England -- Nature. 2009 Oct 8;461(7265):706-7. doi: 10.1038/461706a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19812642" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Cell Aging ; DNA-Directed DNA Polymerase/metabolism ; History, 20th Century ; History, 21st Century ; Humans ; *Nobel Prize ; Saccharomyces cerevisiae/genetics ; Telomerase/genetics/*metabolism ; Telomere/genetics/*metabolism ; Tetrahymena thermophila/genetics ; United States
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  • 56
    Publikationsdatum: 2009-06-19
    Beschreibung: Pluripotency of embryonic stem (ES) cells is controlled by defined transcription factors. During differentiation, mouse ES cells undergo global epigenetic reprogramming, as exemplified by X-chromosome inactivation (XCI) in which one female X chromosome is silenced to achieve gene dosage parity between the sexes. Somatic XCI is regulated by homologous X-chromosome pairing and counting, and by the random choice of future active and inactive X chromosomes. XCI and cell differentiation are tightly coupled, as blocking one process compromises the other and dedifferentiation of somatic cells to induced pluripotent stem cells is accompanied by X chromosome reactivation. Recent evidence suggests coupling of Xist expression to pluripotency factors occurs, but how the two are interconnected remains unknown. Here we show that Oct4 (also known as Pou5f1) lies at the top of the XCI hierarchy, and regulates XCI by triggering X-chromosome pairing and counting. Oct4 directly binds Tsix and Xite, two regulatory noncoding RNA genes of the X-inactivation centre, and also complexes with XCI trans-factors, Ctcf and Yy1 (ref. 17), through protein-protein interactions. Depletion of Oct4 blocks homologous X-chromosome pairing and results in the inactivation of both X chromosomes in female cells. Thus, we have identified the first trans-factor that regulates counting, and ascribed new functions to Oct4 during X-chromosome reprogramming.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057664/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057664/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Donohoe, Mary E -- Silva, Susana S -- Pinter, Stefan F -- Xu, Na -- Lee, Jeannie T -- GM58839/GM/NIGMS NIH HHS/ -- R01 GM058839/GM/NIGMS NIH HHS/ -- R01 GM058839-10/GM/NIGMS NIH HHS/ -- Howard Hughes Medical Institute/ -- England -- Nature. 2009 Jul 2;460(7251):128-32. doi: 10.1038/nature08098. Epub 2009 Jun 17.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19536159" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Cell Line ; *Chromosome Pairing ; Female ; Humans ; Male ; Mice ; Octamer Transcription Factor-3/deficiency/genetics/*metabolism ; Protein Binding ; RNA, Long Noncoding ; RNA, Untranslated/genetics ; Repressor Proteins/*metabolism ; SOXB1 Transcription Factors ; Transcriptional Activation ; X Chromosome/*genetics/*metabolism ; X Chromosome Inactivation/*genetics ; YY1 Transcription Factor/metabolism
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 57
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-04-28
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Byam Shaw, Nicholas -- England -- Nature. 2009 Apr 23;458(7241):984-5. doi: 10.1038/458984a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19396136" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): History, 19th Century ; History, 20th Century ; Literature, Modern/history ; Periodicals as Topic/history ; Publishing/*history
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 58
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-02-20
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tollefson, Jeff -- England -- Nature. 2009 Feb 19;457(7232):942-3. doi: 10.1038/457942b.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19225485" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Engineering ; *Federal Government ; Fishes ; *Greenhouse Effect ; History, 20th Century ; History, 21st Century ; Hobbies/history ; Marine Biology ; Physics ; *Research Personnel ; United States ; United States Government Agencies/*organization & administration ; Wine
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 59
    Publikationsdatum: 2009-04-24
    Beschreibung: Ca(2+) mobilization from intracellular stores represents an important cell signalling process that is regulated, in mammalian cells, by inositol-1,4,5-trisphosphate (InsP(3)), cyclic ADP ribose and nicotinic acid adenine dinucleotide phosphate (NAADP). InsP(3) and cyclic ADP ribose cause the release of Ca(2+) from sarcoplasmic/endoplasmic reticulum stores by the activation of InsP(3) and ryanodine receptors (InsP(3)Rs and RyRs). In contrast, the nature of the intracellular stores targeted by NAADP and the molecular identity of the NAADP receptors remain controversial, although evidence indicates that NAADP mobilizes Ca(2+) from lysosome-related acidic compartments. Here we show that two-pore channels (TPCs) comprise a family of NAADP receptors, with human TPC1 (also known as TPCN1) and chicken TPC3 (TPCN3) being expressed on endosomal membranes, and human TPC2 (TPCN2) on lysosomal membranes when expressed in HEK293 cells. Membranes enriched with TPC2 show high affinity NAADP binding, and TPC2 underpins NAADP-induced Ca(2+) release from lysosome-related stores that is subsequently amplified by Ca(2+)-induced Ca(2+) release by InsP(3)Rs. Responses to NAADP were abolished by disrupting the lysosomal proton gradient and by ablating TPC2 expression, but were only attenuated by depleting endoplasmic reticulum Ca(2+) stores or by blocking InsP(3)Rs. Thus, TPCs form NAADP receptors that release Ca(2+) from acidic organelles, which can trigger further Ca(2+) signals via sarcoplasmic/endoplasmic reticulum. TPCs therefore provide new insights into the regulation and organization of Ca(2+) signals in animal cells, and will advance our understanding of the physiological role of NAADP.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2761823/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2761823/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Calcraft, Peter J -- Ruas, Margarida -- Pan, Zui -- Cheng, Xiaotong -- Arredouani, Abdelilah -- Hao, Xuemei -- Tang, Jisen -- Rietdorf, Katja -- Teboul, Lydia -- Chuang, Kai-Ting -- Lin, Peihui -- Xiao, Rui -- Wang, Chunbo -- Zhu, Yingmin -- Lin, Yakang -- Wyatt, Christopher N -- Parrington, John -- Ma, Jianjie -- Evans, A Mark -- Galione, Antony -- Zhu, Michael X -- 070772/Wellcome Trust/United Kingdom -- FS/05/050/British Heart Foundation/United Kingdom -- P30 NS045758/NS/NINDS NIH HHS/ -- P30 NS045758-05/NS/NINDS NIH HHS/ -- P30 NS045758-059003/NS/NINDS NIH HHS/ -- P30-NS045758/NS/NINDS NIH HHS/ -- R01 DK081654/DK/NIDDK NIH HHS/ -- R01 DK081654-01A1/DK/NIDDK NIH HHS/ -- R01 NS042183/NS/NINDS NIH HHS/ -- R01 NS042183-04/NS/NINDS NIH HHS/ -- R21 NS056942/NS/NINDS NIH HHS/ -- R21 NS056942-01/NS/NINDS NIH HHS/ -- England -- Nature. 2009 May 28;459(7246):596-600. doi: 10.1038/nature08030. Epub 2009 Apr 22.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Centre for Integrative Physiology, College of Medicine and Veterinary Medicine, University of Edinburgh, Hugh Robson Building, Edinburgh EH8 9XD, Scotland, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19387438" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Calcium/*metabolism ; Calcium Channels/genetics/*metabolism ; *Calcium Signaling/drug effects ; Cell Line ; Chickens ; Humans ; Hydrogen-Ion Concentration ; Insulin-Secreting Cells/drug effects/metabolism ; Mice ; Mice, Knockout ; Molecular Sequence Data ; NADP/*analogs & derivatives/metabolism/pharmacology ; Organelles/drug effects/*metabolism ; Protein Binding
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 60
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-04-28
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Campbell, Philip -- England -- Nature. 2009 Apr 23;458(7241):985-6. doi: 10.1038/458985a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19396137" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): *Editorial Policies ; Greenhouse Effect ; History, 20th Century ; *Peer Review, Research/methods ; Periodicals as Topic/*history
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    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 61
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-12-18
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kuper, Adam -- England -- Nature. 2009 Dec 17;462(7275):862. doi: 10.1038/462862a.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Anthropology, Yale University, New Haven, Connecticut 06520, USA. adam.kuper@googlemail.com〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20016590" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Anthropology/*history ; Brazil ; France ; History, 20th Century ; Humans ; Indians, South American
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 62
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-07-10
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hand, Eric -- England -- Nature. 2009 Jul 9;460(7252):161. doi: 10.1038/460161a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19587733" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; *Biological Evolution ; Carbon/metabolism ; Cell Respiration ; *Earth (Planet) ; *Ecosystem ; Fossils ; History, Ancient ; Oceans and Seas ; Oxygen/analysis/*metabolism ; Photosynthesis ; Plants/*metabolism ; Seawater/chemistry
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 63
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-04-21
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Campbell, Philip -- England -- Nature. 2009 Apr 16;458(7240):807. doi: 10.1038/458807a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19378388" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Editorial Policies ; History, 20th Century ; Journalism/*history ; Peer Review, Research ; Periodicals as Topic/*history
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    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 64
    Publikationsdatum: 2009-08-08
    Beschreibung: During the twentieth century, the global population has gone through unprecedented increases in economic and social development that coincided with substantial declines in human fertility and population growth rates. The negative association of fertility with economic and social development has therefore become one of the most solidly established and generally accepted empirical regularities in the social sciences. As a result of this close connection between development and fertility decline, more than half of the global population now lives in regions with below-replacement fertility (less than 2.1 children per woman). In many highly developed countries, the trend towards low fertility has also been deemed irreversible. Rapid population ageing, and in some cases the prospect of significant population decline, have therefore become a central socioeconomic concern and policy challenge. Here we show, using new cross-sectional and longitudinal analyses of the total fertility rate and the human development index (HDI), a fundamental change in the well-established negative relationship between fertility and development as the global population entered the twenty-first century. Although development continues to promote fertility decline at low and medium HDI levels, our analyses show that at advanced HDI levels, further development can reverse the declining trend in fertility. The previously negative development-fertility relationship has become J-shaped, with the HDI being positively associated with fertility among highly developed countries. This reversal of fertility decline as a result of continued economic and social development has the potential to slow the rates of population ageing, thereby ameliorating the social and economic problems that have been associated with the emergence and persistence of very low fertility.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Myrskyla, Mikko -- Kohler, Hans-Peter -- Billari, Francesco C -- England -- Nature. 2009 Aug 6;460(7256):741-3. doi: 10.1038/nature08230.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Population Studies Center, University of Pennsylvania, 3718 Locust Walk, Philadelphia, Pennsylvania 19104, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19661915" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Age Distribution ; *Birth Rate/trends ; Cross-Sectional Studies ; Developed Countries/economics/*statistics & numerical data ; Education ; Female ; Fertility/physiology ; History, 20th Century ; History, 21st Century ; Humans ; Income ; Life Expectancy ; Longitudinal Studies ; Male ; Maternal Age ; *Population Growth ; Reproductive Behavior/history/*statistics & numerical data ; Technology/history/statistics & numerical data/trends
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 65
    Publikationsdatum: 2009-03-20
    Beschreibung: Thirty years after oxygen isotope records from microfossils deposited in ocean sediments confirmed the hypothesis that variations in the Earth's orbital geometry control the ice ages, fundamental questions remain over the response of the Antarctic ice sheets to orbital cycles. Furthermore, an understanding of the behaviour of the marine-based West Antarctic ice sheet (WAIS) during the 'warmer-than-present' early-Pliocene epoch ( approximately 5-3 Myr ago) is needed to better constrain the possible range of ice-sheet behaviour in the context of future global warming. Here we present a marine glacial record from the upper 600 m of the AND-1B sediment core recovered from beneath the northwest part of the Ross ice shelf by the ANDRILL programme and demonstrate well-dated, approximately 40-kyr cyclic variations in ice-sheet extent linked to cycles in insolation influenced by changes in the Earth's axial tilt (obliquity) during the Pliocene. Our data provide direct evidence for orbitally induced oscillations in the WAIS, which periodically collapsed, resulting in a switch from grounded ice, or ice shelves, to open waters in the Ross embayment when planetary temperatures were up to approximately 3 degrees C warmer than today and atmospheric CO(2) concentration was as high as approximately 400 p.p.m.v. (refs 5, 6). The evidence is consistent with a new ice-sheet/ice-shelf model that simulates fluctuations in Antarctic ice volume of up to +7 m in equivalent sea level associated with the loss of the WAIS and up to +3 m in equivalent sea level from the East Antarctic ice sheet, in response to ocean-induced melting paced by obliquity. During interglacial times, diatomaceous sediments indicate high surface-water productivity, minimal summer sea ice and air temperatures above freezing, suggesting an additional influence of surface melt under conditions of elevated CO(2).〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Naish, T -- Powell, R -- Levy, R -- Wilson, G -- Scherer, R -- Talarico, F -- Krissek, L -- Niessen, F -- Pompilio, M -- Wilson, T -- Carter, L -- DeConto, R -- Huybers, P -- McKay, R -- Pollard, D -- Ross, J -- Winter, D -- Barrett, P -- Browne, G -- Cody, R -- Cowan, E -- Crampton, J -- Dunbar, G -- Dunbar, N -- Florindo, F -- Gebhardt, C -- Graham, I -- Hannah, M -- Hansaraj, D -- Harwood, D -- Helling, D -- Henrys, S -- Hinnov, L -- Kuhn, G -- Kyle, P -- Laufer, A -- Maffioli, P -- Magens, D -- Mandernack, K -- McIntosh, W -- Millan, C -- Morin, R -- Ohneiser, C -- Paulsen, T -- Persico, D -- Raine, I -- Reed, J -- Riesselman, C -- Sagnotti, L -- Schmitt, D -- Sjunneskog, C -- Strong, P -- Taviani, M -- Vogel, S -- Wilch, T -- Williams, T -- England -- Nature. 2009 Mar 19;458(7236):322-8. doi: 10.1038/nature07867.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Antarctic Research Centre, Victoria University of Wellington, Kelburn Parade, PO Box 600, Wellington 6012, New Zealand. tim.naish@vuw.ac.nz〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19295607" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Antarctic Regions ; Atmosphere/analysis/chemistry ; Calibration ; Carbon Dioxide/analysis ; Diatoms/chemistry/isolation & purification ; Fossils ; History, Ancient ; *Ice Cover ; Oxygen Isotopes ; Temperature
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 66
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-05-30
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hand, Eric -- England -- Nature. 2009 May 28;459(7246):495. doi: 10.1038/459495a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19478752" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): History, 20th Century ; History, 21st Century ; United States ; United States National Aeronautics and Space Administration/*organization & ; administration
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 67
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-04-03
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Abbott, Alison -- England -- Nature. 2009 Apr 2;458(7238):564-7. doi: 10.1038/458564a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19340056" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Academies and Institutes/economics/trends ; Anniversaries and Special Events ; History, 20th Century ; History, 21st Century ; Italy ; Nerve Growth Factor/*history ; *Nobel Prize ; Silver Staining/history ; United States ; Women's Rights/history/trends
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 68
    Publikationsdatum: 2009-11-10
    Beschreibung: The plant hormone abscisic acid (ABA) has a central role in coordinating the adaptive response in situations of decreased water availability as well as the regulation of plant growth and development. Recently, a 14-member family of intracellular ABA receptors, named PYR/PYL/RCAR, has been identified. These proteins inhibit in an ABA-dependent manner the activity of a family of key negative regulators of the ABA signalling pathway: the group-A protein phosphatases type 2C (PP2Cs). Here we present the crystal structure of Arabidopsis thaliana PYR1, which consists of a dimer in which one of the subunits is bound to ABA. In the ligand-bound subunit, the loops surrounding the entry to the binding cavity fold over the ABA molecule, enclosing it inside, whereas in the empty subunit they form a channel leaving an open access to the cavity, indicating that conformational changes in these loops have a critical role in the stabilization of the hormone-receptor complex. By providing structural details on the ABA-binding pocket, this work paves the way for the development of new small molecules able to activate the plant stress response.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Santiago, Julia -- Dupeux, Florine -- Round, Adam -- Antoni, Regina -- Park, Sang-Youl -- Jamin, Marc -- Cutler, Sean R -- Rodriguez, Pedro Luis -- Marquez, Jose Antonio -- England -- Nature. 2009 Dec 3;462(7273):665-8. doi: 10.1038/nature08591. Epub 2009 Nov 8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Instituto de Biologia Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientificas-Universidad Politecnica de Valencia, ES-46022 Valencia, Spain.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19898494" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Abscisic Acid/*metabolism ; Arabidopsis ; Arabidopsis Proteins/*chemistry/*metabolism ; Membrane Transport Proteins/*chemistry/*metabolism ; *Models, Molecular ; Protein Binding ; Protein Structure, Tertiary
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 69
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-01-23
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Eggleston, Angela K -- England -- Nature. 2009 Jan 22;457(7228):395. doi: 10.1038/457395a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19158784" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Gene Knockdown Techniques/history ; History, 20th Century ; History, 21st Century ; Humans ; MicroRNAs/genetics/metabolism ; Nobel Prize ; *RNA Interference
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 70
    Publikationsdatum: 2009-06-26
    Beschreibung: Dietary restriction extends longevity in diverse species, suggesting that there is a conserved mechanism for nutrient regulation and prosurvival responses. Here we show a role for the HECT (homologous to E6AP carboxy terminus) E3 ubiquitin ligase WWP-1 as a positive regulator of lifespan in Caenorhabditis elegans in response to dietary restriction. We find that overexpression of wwp-1 in worms extends lifespan by up to 20% under conditions of ad libitum feeding. This extension is dependent on the FOXA transcription factor pha-4, and independent of the FOXO transcription factor daf-16. Reduction of wwp-1 completely suppresses the extended longevity of diet-restricted animals. However, the loss of wwp-1 does not affect the long lifespan of animals with compromised mitochondrial function or reduced insulin/IGF-1 signalling. Overexpression of a mutant form of WWP-1 lacking catalytic activity suppresses the increased lifespan of diet-restricted animals, indicating that WWP-1 ubiquitin ligase activity is essential for longevity. Furthermore, we find that the E2 ubiquitin conjugating enzyme, UBC-18, is essential and specific for diet-restriction-induced longevity. UBC-18 interacts with WWP-1 and is required for the ubiquitin ligase activity of WWP-1 and the extended longevity of worms overexpressing wwp-1. Taken together, our results indicate that WWP-1 and UBC-18 function to ubiquitinate substrates that regulate diet-restriction-induced longevity.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2746748/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2746748/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Carrano, Andrea C -- Liu, Zheng -- Dillin, Andrew -- Hunter, Tony -- AG 027463/AG/NIA NIH HHS/ -- AG 032560/AG/NIA NIH HHS/ -- CA 14195/CA/NCI NIH HHS/ -- CA 54418/CA/NCI NIH HHS/ -- CA 82683/CA/NCI NIH HHS/ -- DK 070696/DK/NIDDK NIH HHS/ -- P01 CA054418/CA/NCI NIH HHS/ -- P01 CA054418-110010/CA/NCI NIH HHS/ -- P30 CA014195/CA/NCI NIH HHS/ -- P30 CA014195-35/CA/NCI NIH HHS/ -- R01 AG027463/AG/NIA NIH HHS/ -- R01 AG027463-01A2/AG/NIA NIH HHS/ -- R01 CA082683/CA/NCI NIH HHS/ -- R01 CA082683-07/CA/NCI NIH HHS/ -- R01 CA082683-08/CA/NCI NIH HHS/ -- R01 DK070696/DK/NIDDK NIH HHS/ -- R01 DK070696-04/DK/NIDDK NIH HHS/ -- R21 AG032560/AG/NIA NIH HHS/ -- R21 AG032560-01/AG/NIA NIH HHS/ -- England -- Nature. 2009 Jul 16;460(7253):396-9. doi: 10.1038/nature08130. Epub 2009 Jun 24.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19553937" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Animals, Genetically Modified ; Caenorhabditis elegans/genetics/*physiology ; Caenorhabditis elegans Proteins/genetics/*metabolism ; *Caloric Restriction ; DNA-Binding Proteins/metabolism ; Heat-Shock Response ; Ligases/genetics/*metabolism ; Longevity/*physiology ; Protein Binding ; Receptors, Nicotinic/genetics/metabolism ; Trans-Activators/genetics/metabolism ; Transcription Factors/metabolism ; Ubiquitin-Protein Ligases/genetics/*metabolism ; Ubiquitination/*physiology
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  • 71
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-12-17
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nayar, Anjali -- England -- Nature. 2009 Dec 10;462(7274):717-9. doi: 10.1038/462717a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20010663" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Cameroon/epidemiology ; Communicable Diseases, Emerging/epidemiology/prevention & ; control/*transmission/*virology ; Disease Outbreaks/prevention & control/veterinary ; History, 20th Century ; History, 21st Century ; Humans ; Population Surveillance/*methods ; Zoonoses/epidemiology/*transmission/*virology
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 72
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-01-23
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Abler, William L -- England -- Nature. 2009 Jan 22;457(7228):379. doi: 10.1038/457379d.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19158768" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Bees/physiology ; *Biological Evolution ; History, 20th Century ; Humans
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 73
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-03-13
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Trounson, Alan -- England -- Nature. 2009 Mar 12;458(7235):161. doi: 10.1038/458161a.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉California Institute for Regenerative Medicine, 210 King Street, San Francisco, California 94107, USA. atrounson@cirm.ca.gov〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19279628" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; *Cellular Reprogramming ; China ; Cloning, Organism/*history ; History, 20th Century ; History, 21st Century ; International Cooperation ; Nuclear Transfer Techniques/*history ; United States
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 74
    Publikationsdatum: 2009-05-22
    Beschreibung: Lunar rocks and impact melts, lunar and asteroidal meteorites, and an ancient martian meteorite record thermal metamorphic events with ages that group around and/or do not exceed 3.9 Gyr. That such a diverse suite of solar system materials share this feature is interpreted to be the result of a post-primary-accretion cataclysmic spike in the number of impacts commonly referred to as the late heavy bombardment (LHB). Despite its obvious significance to the preservation of crust and the survivability of an emergent biosphere, the thermal effects of this bombardment on the young Earth remain poorly constrained. Here we report numerical models constructed to probe the degree of thermal metamorphism in the crust in the effort to recreate the effect of the LHB on the Earth as a whole; outputs were used to assess habitable volumes of crust for a possible near-surface and subsurface primordial microbial biosphere. Our analysis shows that there is no plausible situation in which the habitable zone was fully sterilized on Earth, at least since the termination of primary accretion of the planets and the postulated impact origin of the Moon. Our results explain the root location of hyperthermophilic bacteria in the phylogenetic tree for 16S small-subunit ribosomal RNA, and bode well for the persistence of microbial biospheres even on planetary bodies strongly reworked by impacts.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Abramov, Oleg -- Mojzsis, Stephen J -- England -- Nature. 2009 May 21;459(7245):419-22. doi: 10.1038/nature08015.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉University of Colorado, Department of Geological Sciences, 2200 Colorado Avenue, UCB 399, Boulder, Colorado 80309-0399, USA. oleg.abramov@colorado.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19458721" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Bacteria/genetics/*isolation & purification ; *Earth (Planet) ; Ecosystem ; History, Ancient ; Hot Temperature ; *Meteoroids ; *Models, Biological ; *Moon ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Sterilization ; Time Factors
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 75
    Publikationsdatum: 2009-06-12
    Beschreibung: Alkyltransferase-like proteins (ATLs) share functional motifs with the cancer chemotherapy target O(6)-alkylguanine-DNA alkyltransferase (AGT) and paradoxically protect cells from the biological effects of DNA alkylation damage, despite lacking the reactive cysteine and alkyltransferase activity of AGT. Here we determine Schizosaccharomyces pombe ATL structures without and with damaged DNA containing the endogenous lesion O(6)-methylguanine or cigarette-smoke-derived O(6)-4-(3-pyridyl)-4-oxobutylguanine. These results reveal non-enzymatic DNA nucleotide flipping plus increased DNA distortion and binding pocket size compared to AGT. Our analysis of lesion-binding site conservation identifies new ATLs in sea anemone and ancestral archaea, indicating that ATL interactions are ancestral to present-day repair pathways in all domains of life. Genetic connections to mammalian XPG (also known as ERCC5) and ERCC1 in S. pombe homologues Rad13 and Swi10 and biochemical interactions with Escherichia coli UvrA and UvrC combined with structural results reveal that ATLs sculpt alkylated DNA to create a genetic and structural intersection of base damage processing with nucleotide excision repair.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2729916/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2729916/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tubbs, Julie L -- Latypov, Vitaly -- Kanugula, Sreenivas -- Butt, Amna -- Melikishvili, Manana -- Kraehenbuehl, Rolf -- Fleck, Oliver -- Marriott, Andrew -- Watson, Amanda J -- Verbeek, Barbara -- McGown, Gail -- Thorncroft, Mary -- Santibanez-Koref, Mauro F -- Millington, Christopher -- Arvai, Andrew S -- Kroeger, Matthew D -- Peterson, Lisa A -- Williams, David M -- Fried, Michael G -- Margison, Geoffrey P -- Pegg, Anthony E -- Tainer, John A -- CA018137/CA/NCI NIH HHS/ -- CA097209/CA/NCI NIH HHS/ -- CA59887/CA/NCI NIH HHS/ -- GM070662/GM/NIGMS NIH HHS/ -- R01 CA059887/CA/NCI NIH HHS/ -- R01 CA059887-12/CA/NCI NIH HHS/ -- R01 CA059887-13/CA/NCI NIH HHS/ -- R01 GM070662/GM/NIGMS NIH HHS/ -- R01 GM070662-01/GM/NIGMS NIH HHS/ -- R01 GM070662-02/GM/NIGMS NIH HHS/ -- R01 GM070662-03/GM/NIGMS NIH HHS/ -- R01 GM070662-04/GM/NIGMS NIH HHS/ -- R01 GM070662-05/GM/NIGMS NIH HHS/ -- R01 GM070662-06/GM/NIGMS NIH HHS/ -- Cancer Research UK/United Kingdom -- England -- Nature. 2009 Jun 11;459(7248):808-13. doi: 10.1038/nature08076.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Skaggs Institute for Chemical Biology and Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19516334" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Alkyl and Aryl Transferases/*chemistry/*metabolism ; Alkylation ; Binding Sites ; Crystallography, X-Ray ; DNA/chemistry/metabolism ; *DNA Damage ; *DNA Repair ; Guanine/analogs & derivatives/chemistry/metabolism ; Humans ; Models, Molecular ; Protein Binding ; Protein Conformation
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 76
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-08-08
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Adler, Daniel S -- England -- Nature. 2009 Aug 6;460(7256):695-6. doi: 10.1038/460695a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19661905" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Art/history ; Birds/anatomy & histology ; Bone and Bones ; Culture ; Germany ; History, Ancient ; Music/*history
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 77
    Publikationsdatum: 2009-06-19
    Beschreibung: A profound global climate shift took place at the Eocene-Oligocene transition ( approximately 33.5 million years ago) when Cretaceous/early Palaeogene greenhouse conditions gave way to icehouse conditions. During this interval, changes in the Earth's orbit and a long-term drop in atmospheric carbon dioxide concentrations resulted in both the growth of Antarctic ice sheets to approximately their modern size and the appearance of Northern Hemisphere glacial ice. However, palaeoclimatic studies of this interval are contradictory: although some analyses indicate no major climatic changes, others imply cooler temperatures, increased seasonality and/or aridity. Climatic conditions in high northern latitudes over this interval are particularly poorly known. Here we present northern high-latitude terrestrial climate estimates for the Eocene to Oligocene interval, based on bioclimatic analysis of terrestrially derived spore and pollen assemblages preserved in marine sediments from the Norwegian-Greenland Sea. Our data indicate a cooling of approximately 5 degrees C in cold-month (winter) mean temperatures to 0-2 degrees C, and a concomitant increased seasonality before the Oi-1 glaciation event. These data indicate that a cooling component is indeed incorporated in the delta(18)O isotope shift across the Eocene-Oligocene transition. However, the relatively warm summer temperatures at that time mean that continental ice on East Greenland was probably restricted to alpine outlet glaciers.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Eldrett, James S -- Greenwood, David R -- Harding, Ian C -- Huber, Matthew -- England -- Nature. 2009 Jun 18;459(7249):969-73. doi: 10.1038/nature08069.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Shell Exploration and Production UK Ltd, 1 Altens Farm Road, Nigg, Aberdeen, AB12 3FY, UK. james.eldrett@shell.com〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19536261" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): *Climate ; Geologic Sediments/analysis ; Greenland ; History, Ancient ; Ice Cover ; Norway ; Oceans and Seas ; Pollen ; *Seasons ; Spores ; *Temperature
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 78
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-10-30
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tullius, Tom -- England -- Nature. 2009 Oct 29;461(7268):1225-6. doi: 10.1038/4611225a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19865161" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Base Sequence ; DNA/*chemistry/genetics/*metabolism ; DNA-Binding Proteins/chemistry/*metabolism ; Humans ; *Nucleic Acid Conformation ; Protein Binding
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 79
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-09-26
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chakravarti, Aravinda -- England -- Nature. 2009 Sep 24;461(7263):487-8. doi: 10.1038/461487a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19779444" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Asia/ethnology ; Continental Population Groups/genetics/history ; Ethnic Groups/*genetics/history ; Europe/ethnology ; Female ; Founder Effect ; Genetics, Population ; Genome, Human/genetics ; Genomics ; Genotype ; History, 16th Century ; History, 20th Century ; History, Ancient ; Humans ; India ; Language ; Male ; *Phylogeny ; Polymorphism, Single Nucleotide/genetics
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 80
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-07-10
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hartung, Thomas -- England -- Nature. 2009 Jul 9;460(7252):208-12. doi: 10.1038/460208a.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Environmental Health Sciences at the Johns Hopkins University Bloomberg School of Public Health, USA. thartung@jhsph.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19587762" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Adverse Drug Reaction Reporting Systems ; Animals ; False Positive Reactions ; History, 20th Century ; Humans ; Models, Animal ; Rats ; Reproducibility of Results ; Research Design ; Toxicity Tests ; Toxicology/history/*methods/*trends
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 81
    Publikationsdatum: 2009-09-26
    Beschreibung: Reconstructions of atmospheric CO(2) concentrations based on Antarctic ice cores reveal significant changes during the Holocene epoch, but the processes responsible for these changes in CO(2) concentrations have not been unambiguously identified. Distinct characteristics in the carbon isotope signatures of the major carbon reservoirs (ocean, biosphere, sediments and atmosphere) constrain variations in the CO(2) fluxes between those reservoirs. Here we present a highly resolved atmospheric delta(13)C record for the past 11,000 years from measurements on atmospheric CO(2) trapped in an Antarctic ice core. From mass-balance inverse model calculations performed with a simplified carbon cycle model, we show that the decrease in atmospheric CO(2) of about 5 parts per million by volume (p.p.m.v.). The increase in delta(13)C of about 0.25 per thousand during the early Holocene is most probably the result of a combination of carbon uptake of about 290 gigatonnes of carbon by the land biosphere and carbon release from the ocean in response to carbonate compensation of the terrestrial uptake during the termination of the last ice age. The 20 p.p.m.v. increase of atmospheric CO(2) and the small decrease in delta(13)C of about 0.05 per thousand during the later Holocene can mostly be explained by contributions from carbonate compensation of earlier land-biosphere uptake and coral reef formation, with only a minor contribution from a small decrease of the land-biosphere carbon inventory.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Elsig, Joachim -- Schmitt, Jochen -- Leuenberger, Daiana -- Schneider, Robert -- Eyer, Marc -- Leuenberger, Markus -- Joos, Fortunat -- Fischer, Hubertus -- Stocker, Thomas F -- England -- Nature. 2009 Sep 24;461(7263):507-10. doi: 10.1038/nature08393.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Climate and Environmental Physics, Physics Institute, University of Bern, Sidlerstrasse 5, CH-3012 Bern, Switzerland.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19779448" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Air/analysis ; Animals ; Antarctic Regions ; Anthozoa/growth & development/metabolism ; Atmosphere/chemistry ; Carbon/*analysis/*metabolism ; Carbon Dioxide/analysis/*metabolism ; Carbon Isotopes ; Climate ; Ecosystem ; History, Ancient ; Ice Cover/*chemistry ; Time Factors
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 82
    Publikationsdatum: 2009-11-20
    Beschreibung: Allosteric regulation is used as a very efficient mechanism to control protein activity in most biological processes, including signal transduction, metabolism, catalysis and gene regulation. Allosteric proteins can exist in several conformational states with distinct binding or enzymatic activity. Effectors are considered to function in a purely structural manner by selectively stabilizing a specific conformational state, thereby regulating protein activity. Here we show that allosteric proteins can be regulated predominantly by changes in their structural dynamics. We have used NMR spectroscopy and isothermal titration calorimetry to characterize cyclic AMP (cAMP) binding to the catabolite activator protein (CAP), a transcriptional activator that has been a prototype for understanding effector-mediated allosteric control of protein activity. cAMP switches CAP from the 'off' state (inactive), which binds DNA weakly and non-specifically, to the 'on' state (active), which binds DNA strongly and specifically. In contrast, cAMP binding to a single CAP mutant, CAP-S62F, fails to elicit the active conformation; yet, cAMP binding to CAP-S62F strongly activates the protein for DNA binding. NMR and thermodynamic analyses show that despite the fact that CAP-S62F-cAMP(2) adopts the inactive conformation, its strong binding to DNA is driven by a large conformational entropy originating in enhanced protein motions induced by DNA binding. The results provide strong evidence that changes in protein motions may activate allosteric proteins that are otherwise structurally inactive.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tzeng, Shiou-Ru -- Kalodimos, Charalampos G -- England -- Nature. 2009 Nov 19;462(7271):368-72. doi: 10.1038/nature08560.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry & Chemical Biology, Rutgers University, Piscataway, New Jersey 08854, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19924217" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Cyclic AMP/chemistry/metabolism ; Cyclic AMP Receptor Protein/chemistry/*metabolism ; DNA/metabolism ; *Energy Metabolism ; Escherichia coli/*metabolism ; Escherichia coli Proteins/chemistry/*metabolism ; Models, Molecular ; Protein Binding ; Protein Structure, Tertiary
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 83
    Publikationsdatum: 2009-07-15
    Beschreibung: Recent finds demonstrate that internal fertilization and viviparity (live birth) were more widespread in the Placodermi, an extinct group of armoured fishes, than was previously realized. Placoderms represent the sister group of the crown group jawed vertebrates (Gnathostomata), making their mode(s) of reproduction potentially informative about primitive gnathostome conditions. An ossified pelvic fin basipterygium discovered in the arthrodire Incisoscutum ritchiei was hypothesized to be identical in males and females, with males presumed to have an additional cartilaginous element or series forming a clasper. Here we report the discovery of a completely ossified pelvic clasper in Incisoscutum ritchiei (WAM 03.3.28) which shows that this interpretation was incorrect: the basipterygium described previously is in fact unique to females. The male clasper is a slender rod attached to a square basal plate that articulates directly with the pelvis. It carries a small cap of dermal bone covered in denticles and small hooks that may be homologous with the much larger dermal component of the ptyctodont clasper.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ahlberg, Per -- Trinajstic, Kate -- Johanson, Zerina -- Long, John -- England -- Nature. 2009 Aug 13;460(7257):888-9. doi: 10.1038/nature08176. Epub 2009 Jul 13.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Subdepartment of Evolutionary Organismal Biology, Department of Physiology and Developmental Biology, Uppsala University, Norbyvagen 18A, 752 36 Uppsala, Sweden.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19597477" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animal Structures/anatomy & histology/*physiology ; Animals ; Female ; Fertilization/*physiology ; Fishes/*anatomy & histology/*physiology ; Fossils ; History, Ancient ; Male ; Pelvis/anatomy & histology ; Viviparity, Nonmammalian/physiology
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 84
    Publikationsdatum: 2009-12-01
    Beschreibung: Type IV secretion systems are secretion nanomachines spanning the two membranes of Gram-negative bacteria. Three proteins, VirB7, VirB9 and VirB10, assemble into a 1.05 megadalton (MDa) core spanning the inner and outer membranes. This core consists of 14 copies of each of the proteins and forms two layers, the I and O layers, inserting in the inner and outer membrane, respectively. Here we present the crystal structure of a approximately 0.6 MDa outer-membrane complex containing the entire O layer. This structure is the largest determined for an outer-membrane channel and is unprecedented in being composed of three proteins. Unexpectedly, this structure identifies VirB10 as the outer-membrane channel with a unique hydrophobic double-helical transmembrane region. This structure establishes VirB10 as the only known protein crossing both membranes of Gram-negative bacteria. Comparison of the cryo-electron microscopy (cryo-EM) and crystallographic structures points to conformational changes regulating channel opening and closing.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2797999/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2797999/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chandran, Vidya -- Fronzes, Remi -- Duquerroy, Stephane -- Cronin, Nora -- Navaza, Jorge -- Waksman, Gabriel -- 082227/Wellcome Trust/United Kingdom -- England -- Nature. 2009 Dec 24;462(7276):1011-5. doi: 10.1038/nature08588. Epub 2009 Nov 29.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of Structural and Molecular Biology, University College London and Birkbeck College, Malet Street, London WC1E 7HX, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19946264" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Bacterial Outer Membrane Proteins/*chemistry/isolation & purification ; Gram-Negative Bacteria/*chemistry/*physiology ; *Models, Molecular ; Protein Binding ; Protein Structure, Quaternary
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  • 85
    Publikationsdatum: 2009-02-27
    Beschreibung: A pathological hallmark of Alzheimer's disease is an accumulation of insoluble plaque containing the amyloid-beta peptide of 40-42 amino acid residues. Prefibrillar, soluble oligomers of amyloid-beta have been recognized to be early and key intermediates in Alzheimer's-disease-related synaptic dysfunction. At nanomolar concentrations, soluble amyloid-beta oligomers block hippocampal long-term potentiation, cause dendritic spine retraction from pyramidal cells and impair rodent spatial memory. Soluble amyloid-beta oligomers have been prepared from chemical syntheses, transfected cell culture supernatants, transgenic mouse brain and human Alzheimer's disease brain. Together, these data imply a high-affinity cell-surface receptor for soluble amyloid-beta oligomers on neurons-one that is central to the pathophysiological process in Alzheimer's disease. Here we identify the cellular prion protein (PrP(C)) as an amyloid-beta-oligomer receptor by expression cloning. Amyloid-beta oligomers bind with nanomolar affinity to PrP(C), but the interaction does not require the infectious PrP(Sc) conformation. Synaptic responsiveness in hippocampal slices from young adult PrP null mice is normal, but the amyloid-beta oligomer blockade of long-term potentiation is absent. Anti-PrP antibodies prevent amyloid-beta-oligomer binding to PrP(C) and rescue synaptic plasticity in hippocampal slices from oligomeric amyloid-beta. Thus, PrP(C) is a mediator of amyloid-beta-oligomer-induced synaptic dysfunction, and PrP(C)-specific pharmaceuticals may have therapeutic potential for Alzheimer's disease.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2748841/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2748841/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lauren, Juha -- Gimbel, David A -- Nygaard, Haakon B -- Gilbert, John W -- Strittmatter, Stephen M -- 5T32GN07205/PHS HHS/ -- P30 DA018343/DA/NIDA NIH HHS/ -- R01 NS039962/NS/NINDS NIH HHS/ -- R01 NS039962-09/NS/NINDS NIH HHS/ -- R01 NS042304/NS/NINDS NIH HHS/ -- R01 NS042304-08/NS/NINDS NIH HHS/ -- R37 NS033020/NS/NINDS NIH HHS/ -- R37 NS033020-17/NS/NINDS NIH HHS/ -- England -- Nature. 2009 Feb 26;457(7233):1128-32. doi: 10.1038/nature07761.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cellular Neuroscience, Neurodegeneration and Repair Program, Yale University School of Medicine, New Haven, Connecticut 06536, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19242475" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Alzheimer Disease/metabolism/pathology/physiopathology ; Amyloid Precursor Protein Secretases/metabolism ; Amyloid beta-Peptides/*chemistry/*metabolism ; Amyloidosis/metabolism ; Animals ; COS Cells ; Cercopithecus aethiops ; Hippocampus/cytology/metabolism ; Humans ; Long-Term Potentiation/physiology ; Mice ; Mice, Inbred C57BL ; *Neuronal Plasticity ; Neurons/metabolism ; Peptide Fragments/*chemistry/*metabolism ; Prions/genetics/*metabolism ; Protein Binding ; *Protein Multimerization ; Receptors, Cell Surface/genetics/metabolism ; Synapses/*metabolism/*pathology
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 86
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-10-02
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Schiermeier, Quirin -- England -- Nature. 2009 Oct 1;461(7264):590-1. doi: 10.1038/461590a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19794471" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Europe, Eastern ; History, 20th Century ; History, 21st Century ; Research Personnel/statistics & numerical data ; Science/*economics/history/manpower/standards
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 87
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-10-02
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Schiermeier, Quirin -- England -- Nature. 2009 Oct 1;461(7264):586-9. doi: 10.1038/461586a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19794470" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Berlin ; Communism/history ; Czechoslovakia ; Democracy ; Education, Graduate ; Europe, Eastern ; Financing, Organized ; History, 20th Century ; History, 21st Century ; Humans ; Internationality ; Israel ; Poland ; Politics ; Research Personnel/economics/*education/*history/standards ; Switzerland ; United States
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 88
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-07-17
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Charron, Dominique J -- England -- Nature. 2009 Jul 16;460(7253):338. doi: 10.1038/460338a.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratoire Jean Dausset, Hopital Saint-Louis, and the Institut Universitaire d'Hematologie, 75010 Paris, France. dominique.charron@sls.aphp.fr〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19606138" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Allergy and Immunology/*history ; France ; Genetic Predisposition to Disease ; HLA Antigens/genetics/*history/*immunology ; History, 20th Century ; Humans ; Polymorphism, Genetic ; Transplantation Immunology
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 89
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-10-16
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hayden, Erika Check -- England -- Nature. 2009 Oct 15;461(7266):859. doi: 10.1038/461859a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19829341" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Algorithms ; Computational Biology/*standards ; *Computer Simulation ; Drug Design ; Ligands ; Protein Binding ; *Protein Stability ; Reproducibility of Results ; Research Personnel/ethics/standards ; *Retraction of Publication as Topic ; Scientific Misconduct ; *Substrate Specificity
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 90
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-07-08
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nicotera, Pierluigi -- MC_U132664972/Medical Research Council/United Kingdom -- England -- Nature. 2009 Jul 2;460(7251):135. doi: 10.1038/nj7251-135a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19582898" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Biomedical Research/*history/*organization & administration ; Germany ; History, 20th Century ; History, 21st Century ; Humans ; *Neurodegenerative Diseases/metabolism/pathology
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 91
    Publikationsdatum: 2009-02-20
    Beschreibung: Naturally occurring axonal pruning and neuronal cell death help to sculpt neuronal connections during development, but their mechanistic basis remains poorly understood. Here we report that beta-amyloid precursor protein (APP) and death receptor 6 (DR6, also known as TNFRSF21) activate a widespread caspase-dependent self-destruction program. DR6 is broadly expressed by developing neurons, and is required for normal cell body death and axonal pruning both in vivo and after trophic-factor deprivation in vitro. Unlike neuronal cell body apoptosis, which requires caspase 3, we show that axonal degeneration requires caspase 6, which is activated in a punctate pattern that parallels the pattern of axonal fragmentation. DR6 is activated locally by an inactive surface ligand(s) that is released in an active form after trophic-factor deprivation, and we identify APP as a DR6 ligand. Trophic-factor deprivation triggers the shedding of surface APP in a beta-secretase (BACE)-dependent manner. Loss- and gain-of-function studies support a model in which a cleaved amino-terminal fragment of APP (N-APP) binds DR6 and triggers degeneration. Genetic support is provided by a common neuromuscular junction phenotype in mutant mice. Our results indicate that APP and DR6 are components of a neuronal self-destruction pathway, and suggest that an extracellular fragment of APP, acting via DR6 and caspase 6, contributes to Alzheimer's disease.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2677572/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2677572/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nikolaev, Anatoly -- McLaughlin, Todd -- O'Leary, Dennis D M -- Tessier-Lavigne, Marc -- R01 AG025970/AG/NIA NIH HHS/ -- R01 EY007025/EY/NEI NIH HHS/ -- R01 EY007025-24/EY/NEI NIH HHS/ -- R01 EY07025/EY/NEI NIH HHS/ -- England -- Nature. 2009 Feb 19;457(7232):981-9. doi: 10.1038/nature07767.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Research, Genentech, Inc., 1 DNA Way, South San Francisco, California 94080, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19225519" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Alzheimer Disease/metabolism ; Amyloid beta-Protein Precursor/chemistry/*metabolism ; Animals ; Axons/*metabolism ; Caspase 3/metabolism ; Caspase 6/*metabolism ; Caspases/*metabolism ; Cell Death ; Ligands ; Mice ; Neurons/*cytology/*metabolism ; Peptide Fragments/chemistry/metabolism ; Protein Binding ; Receptors, Tumor Necrosis Factor/*metabolism ; Signal Transduction ; bcl-2-Associated X Protein/genetics/metabolism
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 92
    Publikationsdatum: 2009-06-02
    Beschreibung: Diverse histone modifications are catalysed and recognized by various specific proteins, establishing unique modification patterns that act as transcription signals. In particular, histone H3 trimethylation at lysine 36 (H3K36me3) is associated with actively transcribed regions and has been proposed to provide landmarks for continuing transcription; however, the control mechanisms and functions of H3K36me3 in higher eukaryotes are unknown. Here we show that the H3K36me3-specific histone methyltransferase (HMTase) Wolf-Hirschhorn syndrome candidate 1 (WHSC1, also known as NSD2 or MMSET) functions in transcriptional regulation together with developmental transcription factors whose defects overlap with the human disease Wolf-Hirschhorn syndrome (WHS). We found that mouse Whsc1, one of five putative Set2 homologues, governed H3K36me3 along euchromatin by associating with the cell-type-specific transcription factors Sall1, Sall4 and Nanog in embryonic stem cells, and Nkx2-5 in embryonic hearts, regulating the expression of their target genes. Whsc1-deficient mice showed growth retardation and various WHS-like midline defects, including congenital cardiovascular anomalies. The effects of Whsc1 haploinsufficiency were increased in Nkx2-5 heterozygous mutant hearts, indicating their functional link. We propose that WHSC1 functions together with developmental transcription factors to prevent the inappropriate transcription that can lead to various pathophysiologies.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nimura, Keisuke -- Ura, Kiyoe -- Shiratori, Hidetaka -- Ikawa, Masato -- Okabe, Masaru -- Schwartz, Robert J -- Kaneda, Yasufumi -- England -- Nature. 2009 Jul 9;460(7252):287-91. doi: 10.1038/nature08086. Epub 2009 May 31.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Gene Therapy Science, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19483677" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; DNA-Binding Proteins/metabolism ; Gene Expression Regulation ; Histone-Lysine N-Methyltransferase/deficiency/genetics/*metabolism ; Histones/*metabolism ; Homeodomain Proteins/genetics/*metabolism ; Lysine/metabolism ; Methylation ; Mice ; Mice, Inbred C57BL ; Protein Binding ; Repressor Proteins/metabolism ; Transcription Factors/genetics/*metabolism ; Transcription, Genetic ; Wolf-Hirschhorn Syndrome/*metabolism
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 93
    Publikationsdatum: 2009-05-02
    Beschreibung: Global efforts to mitigate climate change are guided by projections of future temperatures. But the eventual equilibrium global mean temperature associated with a given stabilization level of atmospheric greenhouse gas concentrations remains uncertain, complicating the setting of stabilization targets to avoid potentially dangerous levels of global warming. Similar problems apply to the carbon cycle: observations currently provide only a weak constraint on the response to future emissions. Here we use ensemble simulations of simple climate-carbon-cycle models constrained by observations and projections from more comprehensive models to simulate the temperature response to a broad range of carbon dioxide emission pathways. We find that the peak warming caused by a given cumulative carbon dioxide emission is better constrained than the warming response to a stabilization scenario. Furthermore, the relationship between cumulative emissions and peak warming is remarkably insensitive to the emission pathway (timing of emissions or peak emission rate). Hence policy targets based on limiting cumulative emissions of carbon dioxide are likely to be more robust to scientific uncertainty than emission-rate or concentration targets. Total anthropogenic emissions of one trillion tonnes of carbon (3.67 trillion tonnes of CO(2)), about half of which has already been emitted since industrialization began, results in a most likely peak carbon-dioxide-induced warming of 2 degrees C above pre-industrial temperatures, with a 5-95% confidence interval of 1.3-3.9 degrees C.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Allen, Myles R -- Frame, David J -- Huntingford, Chris -- Jones, Chris D -- Lowe, Jason A -- Meinshausen, Malte -- Meinshausen, Nicolai -- England -- Nature. 2009 Apr 30;458(7242):1163-6. doi: 10.1038/nature08019.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physics, University of Oxford, OX1 3PU, UK. myles.allen@physics.ox.ac.uk〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19407800" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Atmosphere/*chemistry ; Benchmarking ; Carbon/*analysis ; Carbon Dioxide/*analysis ; Computer Simulation ; *Greenhouse Effect ; History, 18th Century ; History, 19th Century ; History, 20th Century ; History, 21st Century ; Human Activities/history ; Industry/history ; *Models, Theoretical ; *Temperature ; Time Factors ; Uncertainty
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 94
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-10-16
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Van Noorden, Richard -- England -- Nature. 2009 Oct 15;461(7266):860. doi: 10.1038/461860a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19829342" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Anti-Bacterial Agents/pharmacology ; *Chemistry/history ; Crystallization/history ; *Crystallography, X-Ray/history ; History, 20th Century ; History, 21st Century ; Humans ; *Nobel Prize ; Ribosomes/*chemistry/drug effects/metabolism
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 95
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-06-06
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fara, Patricia -- England -- Nature. 2009 Jun 4;459(7247):642-4. doi: 10.1038/459642a.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Clare College, University of Cambridge CB2 1TL, UK. pf10006@cam.ac.uk〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19494897" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; History, 17th Century ; History, 18th Century ; History, 19th Century ; History, 20th Century ; History, 21st Century ; Humans ; Microscopy/*history/*instrumentation/standards
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 96
    Publikationsdatum: 2009-02-06
    Beschreibung: The largest extant snakes live in the tropics of South America and southeast Asia where high temperatures facilitate the evolution of large body sizes among air-breathing animals whose body temperatures are dependant on ambient environmental temperatures (poikilothermy). Very little is known about ancient tropical terrestrial ecosystems, limiting our understanding of the evolution of giant snakes and their relationship to climate in the past. Here we describe a boid snake from the oldest known neotropical rainforest fauna from the Cerrejon Formation (58-60 Myr ago) in northeastern Colombia. We estimate a body length of 13 m and a mass of 1,135 kg, making it the largest known snake. The maximum size of poikilothermic animals at a given temperature is limited by metabolic rate, and a snake of this size would require a minimum mean annual temperature of 30-34 degrees C to survive. This estimate is consistent with hypotheses of hot Palaeocene neotropics with high concentrations of atmospheric CO(2) based on climate models. Comparison of palaeotemperature estimates from the equator to those from South American mid-latitudes indicates a relatively steep temperature gradient during the early Palaeogene greenhouse, similar to that of today. Depositional environments and faunal composition of the Cerrejon Formation indicate an anaconda-like ecology for the giant snake, and an earliest Cenozoic origin of neotropical vertebrate faunas.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Head, Jason J -- Bloch, Jonathan I -- Hastings, Alexander K -- Bourque, Jason R -- Cadena, Edwin A -- Herrera, Fabiany A -- Polly, P David -- Jaramillo, Carlos A -- England -- Nature. 2009 Feb 5;457(7230):715-7. doi: 10.1038/nature07671.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biology, University of Toronto, Mississauga, Ontario L5L 1C6, Canada. jason.head@utoronto.ca〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19194448" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Atmosphere/chemistry ; Biological Evolution ; *Body Size ; Body Temperature Regulation ; Boidae/*anatomy & histology/metabolism ; Carbon Dioxide/analysis ; Colombia ; Energy Metabolism ; *Fossils ; History, Ancient ; *Temperature ; *Tropical Climate
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    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 97
    Publikationsdatum: 2009-03-28
    Beschreibung: Rivers are the dominant source of many elements and isotopes to the ocean. But this input from the continents is not balanced by the loss of the elements and isotopes through hydrothermal and sedimentary exchange with the oceanic crust, or by temporal changes in the marine inventory for elements that are demonstrably not in steady state. To resolve the problem of the observed imbalance in marine geochemical budgets, attention has been focused on uncertainties in the hydrothermal and sedimentary fluxes. In recent Earth history, temporally dynamic chemical weathering fluxes from the continents are an inevitable consequence of periodic glaciations. Chemical weathering rates on modern Earth are likely to remain far from equilibrium owing to the physical production of finely ground material at glacial terminations that acts as a fertile substrate for chemical weathering. Here we explore the implications of temporal changes in the riverine chemical weathering flux for oceanic geochemical budgets. We contend that the riverine flux obtained from observations of modern rivers is broadly accurate, but not representative of timescales appropriate for elements with oceanic residence longer than Quaternary glacial-interglacial cycles. We suggest that the pulse of rapid chemical weathering initiated at the last deglaciation has not yet decayed away and that weathering rates remain about two to three times the average for an entire late Quaternary glacial cycle. Taking into account the effect of the suggested non-steady-state process on the silicate weathering flux helps to reconcile the modelled marine strontium isotope budget with available data. Overall, we conclude that consideration of the temporal variability in riverine fluxes largely ameliorates long-standing problems with chemical and isotopic mass balances in the ocean.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Vance, Derek -- Teagle, Damon A H -- Foster, Gavin L -- England -- Nature. 2009 Mar 26;458(7237):493-6. doi: 10.1038/nature07828.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Bristol Isotope Group, Department of Earth Sciences, University of Bristol, Wills Memorial Building, Bristol BS8 1RJ, UK. d.vance@bristol.ac.uk〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19325631" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Atmosphere/chemistry ; Carbon Dioxide/analysis ; Carbonates/analysis/chemistry ; Geologic Sediments/*chemistry ; History, Ancient ; Ice Cover ; Osmium/analysis ; Rivers/*chemistry ; Seawater/*chemistry ; Strontium/analysis/chemistry ; Strontium Isotopes ; Temperature
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 98
    Publikationsdatum: 2009-04-11
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Heckenberger, Michael -- England -- Nature. 2009 Apr 9;458(7239):683. doi: 10.1038/458683e.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉University of Florida, Gainesville, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19360041" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Archaeology ; Civilization/*history ; History, Ancient ; History, Medieval ; Humans ; Peru ; Urbanization/*history
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 99
    facet.materialart.
    Unbekannt
    Nature Publishing Group (NPG)
    Publikationsdatum: 2009-05-30
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Vance, Erik -- England -- Nature. 2009 May 28;459(7246):498-9. doi: 10.1038/459498a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19478760" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Aerosols/analysis/chemistry ; Agriculture ; Air Pollutants/analysis/chemistry ; Altitude ; Atmosphere/*chemistry ; California ; History, 20th Century ; History, 21st Century ; Ozone/analysis/chemistry ; Trees/chemistry/*metabolism ; Volatile Organic Compounds/*analysis/chemistry
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 100
    Publikationsdatum: 2009-02-20
    Beschreibung: The complement system is an essential component of the innate and acquired immune system, and consists of a series of proteolytic cascades that are initiated by the presence of microorganisms. In health, activation of complement is precisely controlled through membrane-bound and soluble plasma-regulatory proteins including complement factor H (fH; ref. 2), a 155 kDa protein composed of 20 domains (termed complement control protein repeats). Many pathogens have evolved the ability to avoid immune-killing by recruiting host complement regulators and several pathogens have adapted to avoid complement-mediated killing by sequestering fH to their surface. Here we present the structure of a complement regulator in complex with its pathogen surface-protein ligand. This reveals how the important human pathogen Neisseria meningitidis subverts immune responses by mimicking the host, using protein instead of charged-carbohydrate chemistry to recruit the host complement regulator, fH. The structure also indicates the molecular basis of the host-specificity of the interaction between fH and the meningococcus, and informs attempts to develop novel therapeutics and vaccines.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2670278/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2670278/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Schneider, Muriel C -- Prosser, Beverly E -- Caesar, Joseph J E -- Kugelberg, Elisabeth -- Li, Su -- Zhang, Qian -- Quoraishi, Sadik -- Lovett, Janet E -- Deane, Janet E -- Sim, Robert B -- Roversi, Pietro -- Johnson, Steven -- Tang, Christoph M -- Lea, Susan M -- 083599/Wellcome Trust/United Kingdom -- G0400775/Medical Research Council/United Kingdom -- G0400775(71657)/Medical Research Council/United Kingdom -- G0500367/Medical Research Council/United Kingdom -- G0601195/Medical Research Council/United Kingdom -- G0601195(79743)/Medical Research Council/United Kingdom -- Medical Research Council/United Kingdom -- Wellcome Trust/United Kingdom -- England -- Nature. 2009 Apr 16;458(7240):890-3. doi: 10.1038/nature07769. Epub 2009 Feb 18.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Centre for Molecular Microbiology and Infection, Imperial College, London SW7 2AZ, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19225461" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Antigens, Bacterial/*chemistry/*metabolism ; Bacterial Proteins/*chemistry/*metabolism ; Binding Sites ; Carbohydrates/*chemistry ; Complement Factor H/*chemistry/immunology/*metabolism ; Crystallography, X-Ray ; Ligands ; Models, Molecular ; *Molecular Mimicry ; Neisseria meningitidis/chemistry/immunology/*metabolism ; Nuclear Magnetic Resonance, Biomolecular ; Protein Binding ; Protein Conformation ; Structure-Activity Relationship ; Substrate Specificity
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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