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  • 1
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25268 | 18721 | 2018-09-07 07:30:30 | 25268 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Marine polychaete worms living in different beds from coastal areas, so different cultures can be different effects on their development. The evaluation of this research initially, marine polychaete worms were collected in low tidal sea areas from Gulf Persian coast in Bushehr. During several stages of sampling do more than 2000 pieces of worm were taken and then transferred to Bandarga Shrimp Research Station (Iran Shrimp Research Center). So based on an observation the desired species identification (Perinereis nuntia). Then 400 pieces worm selected and transferred to tanks maintain immature worm (fiberglass tanks with dimensions of 2×2×0.5 m with 15 cm sand on floor). The results showed that even the worms (P. nuntia) in vitro reached sexual maturity able to spawn in captivity conditions and were absolute fecundity of 16010 ± 17630 eggs. After incubation eggs, larval culture into the plastic tank with 70 cm diameter, larval (1.5 to 2 mm) were introduced with density 2000 (pieces/m^2). In this research used to three different beds (tidal, filled with water all of time and wet bed) with three different food types (food shrimp, seaweed and vegetable waste), so there were 9 experimental treatments. All experimental treatments harvested and estimate production in each of them after 270 days. The seaweed tidal treatment with amount of 21.17 g/m^2 had best results and the all of wet treatments with 0 g/m^2 was the lowest production. Compare of beds showed that the tidal treatments have significant difference with other beds in growth factors (e.g. mean weight, survival percentage and total production) (P〈0.05). And for effect of food kind on growth factors, the result of difference foods in tidal treatment were analyzed. According this mater any treatments have no significant difference together in growth factors (e.g. mean weight, survival percentage and total production) (P〉0.05).
    Keywords: Aquaculture ; Iran ; Persian Gulf ; Bushehr Province ; Propagation ; Culture ; Marine ; Worm ; Perinereis nuntia ; Polychaete ; Sampling ; Shrimp ; Perinereis nuntia ; Immature ; P. nuntia ; Sexual maturity ; Survival percentage
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  • 2
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25112 | 18721 | 2018-08-23 05:48:23 | 25112 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Menmiopsis leidyi was entered into Caspian Sea from Black Sea and mass population constitutes in the southern Caspian Sea. In this surveys, the relationship between length and wet weight measured and length and weight followed by this equation: n=384, R^2=0.87 w=0.003L^9005 . The relationship of dry and wet weight calculated, in this surveys, the ratio of dry weight to wet weight was equal to 1.615% in 12 salinity. In vitro, the clearance rate at 12 and 27 C were 52.48 and 107.33 ml/h, respectively, daily ration at above temperatures were 1.98 and 16.65 respectively. Mnemiopsis leidyi can feed Acartia in accordance to 16.5 mg/1gbw (body weight) at 27 C in one day. The average digestive time in 12 C and 27 C were 77.50 min and 36 min respectively. The digestive value in 27 C was 2.5 fold than 12 C. The result indicated that clearance rate, daily ration and digest were increased in high temperature. Stomach content of Mnemiopsis leidyi was shown that this organism utilized from Acartia and Balanus, in addition, stomach content of kilka including two species of zooplankton from the southern Caspian Sea. Stomach contents of clupeonella cultiventris contain more than 54 percent of Acartia and its nauplii. This frequency observed in cluponella engrauliformis and clupeonella grimmi. On basis of zooplankton sampling in the sea, over 90 percentage of zooplankton populations contain Acartia.
    Keywords: Biology ; Iran ; Caspian Sea ; Menmiopsis leidyi ; Zooplankton ; Population ; Survey ; Feeding ; Cetenophora
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  • 3
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25324 | 18721 | 2018-09-12 09:56:53 | 25324 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was conducted in order to optimizing of the biological condition and enhancing of the harvest efficiency for cultured shrimps of Guater site. Experimental design was consisted of three treatments and each treatment with three replications (totally 9 ponds with 600 m^2 area for each pond). After preparing and watering of ponds, the ponds was stocked with shrimp post larves in a density of 0.007±0.001 g (or 20 numbers per m^2) per m^2. After 35 days, the fingerlings of gray mullet were released to shrimp ponds in densities of 0/100 m^2 (T1/), 2/100 m^2 (T2) and 4/100 m^2 (T3). During experiment, the physicochemical parameters of water (temperature, O_2, pH, water transparency and salinity) were measured daily for two times (morning and afternoon). Also, the nitrate, nitrite, ammonia, phosphate and silicate concentrations and BOD5 as well as chlorophyll (a), phytoplanktons and zooplanktons were measured every 15 days one time. To determine the organic values of the bottom sediment of the ponds, monthly sampling was conducted. Health status of shrimps was investigated monthly too. Growth rate, mean weight, survival value, Food conversion ratios (FCR) and total shrimp harvest (Kg) were measured after 107 days rearing in ponds. ANOVA analysis showed significant differences between treatments. The highest (212 Kg, 3533 Kg; weight mean was18.4 g for each shrimp) and lowest (187 Kg, 3116 Kg; weight mean was16.23 g for each shrimp) of harvested shrimps were yielded in T3 and T1 respectively. There was not significant differences between treatments in terms of survival rate (P〉0.05). After 107 days rearing, the highest (1.27 0.2) and lowest (1.2 0.1) of FCR were observed in T_3 and T_1 respectively. Although, there were no significant differences between T_2 and T_3 in ammonia, O_2, Total organic material, pH and BOD5, but, T_2 and T_3 had significant differences with T_1 in these parameters. In this study, 27 genus of phytoplanktons belonged to Diatoms (Bacillariophyceae), blue-green algal (Cyanophyceae) and dinoflagellates (Dinophyceae) were identified. Diatoms with 20 genus had more abundance than dinoflagellates (6 genus) and blue-green algal (1 genus). Also, 7 orders of zooplanktons including copepod, mollusks, nauplius of crustacean and rhizopoda were identified. Health investigation of T1 (without shrimp) showed more pathogenic pollution (parasites and bacteria) than in treatments with shrimp. In this regard, among bacteria, the Vibrio genus (V.Alginoliticus and V.Fluvialis) had more abundance and among parasites zoothamnium sp and Epistylis sp were identified which more abundance was for zoothamnium sp. In conclusion, our results concluded that mixed culturing of white shrimp and grey mullet in optimum densities is possible and is caused more production of shrimp.
    Keywords: Aquaculture ; Iran ; Chabahar ; Guater ; Litopenaeus vannamei ; Mugil cephalus ; Mixed culturing ; Polyculture ; Earthen ponds ; Shrimp ; Fingerlings ; Gray mullet ; Temperature ; O2 ; pH ; Water transparency ; Salinity ; Chlorophyll a ; Phytoplanktons ; Zooplanktons ; ANOVA ; Diatoms ; Bacillariophyceae ; Blue-green algae ; Cyanophyceae ; Copepod ; Investigation
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  • 4
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25327 | 18721 | 2018-09-12 09:54:28 | 25327 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Present project was conducted in shrimp farm located in east of Chabahar in Sistan and Balouchestan province.Twentieth sampling stations at three locations (i.e. 2 stations in supply channel,13 stations in drainage channel and 5 stations in Gwatar Gulf) were selected to determine physical and chemical factors such as; temperature, salinity, dissolved oxygen, pH, nitrate, nitrite, ammonia, phosphate and biological parameters for example Chlorophyll a, phytoplankton and zooplankton. Sampling was conducted once a month from March 2003 until January 2004. The results revealed different bioenvironmental area in the three locations (i.e. supply channel, drainage channel and Gwatar Gulf) over the study period. Concentration of salinity ,pH , nitatr ,nitrite ,ammonia and chlorophyll a in drainage channel were more than supply channel and Gawtar Gulf. While concentation dissolved oxygen and phosphate were in drainage channel less than Gawtar Gulf.Also, sediment with small grain size (clay and silt) caused uptake phosphate and amount phosphate decreased in drainage channel. Considering with lack of environment standards in Iran shrimp effluent, data compared with others countries standard. Results showed that with considerable farm area, value of temperature, salinity, dissolved oxygen and pH were in permit range in Gwatar Gulf while concentation nitrate, phosphate and chlorophyll a were sometimes more than permit range that could cause plankton bloom. Also, Gwatar Gulf were influence monsoon. Nitate, phosphate, chlorophyll a and phytoplankton increased in gwatar Gulf.Regards with that postmonsoon was simultaneus with shrimp harvest.Therefore, increase some parameters could be caused from shrimp effluent or postmonsoon. Thirty four phytoplankton genus were identified including diatoma, dinoflagellate, cyanophyta and chlorophyta. In each area maxiumum percentage of phytoplankton were belonged to diatoma (63%) in supply channel, cyanophyta (51.7%) in drainage channel and dinoflagellate (94.5%) in Gwatar Gulf.Amount and range chemical and physical factors and plankton composition were different in drainage channel, supply channel and Gwatar Gulf. Cyanophyta could grow more in drainage channel.Percentage of aboundance and diversity of phytoplankton changed in 2002 inrelation to years ago( before shrimp culture, 2000 year and 2001 year) specially in Gwatar Gulf. Mainly percentage of zooplankton were copepoda and thintinida respectively.Diversity zooplankton decreased ratio to years ago. In drainage channel, abundance total of phytoplankton and zooplankton were more than Gwatar Gulf and supply channel.
    Keywords: Aquaculture ; Iran ; Sistan and Blouchestan ; Gwatar ; Chabahar ; Environmental effect ; Shrimp culture ; Survey ; Sampling ; Temperature ; Salinity ; Dissolved oxygen ; pH ; Chlorophyll a ; Phytoplankton ; Zooplankton ; Diatoma ; Cyanophyta ; Chlorophyta ; Copepoda
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  • 5
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25333 | 18721 | 2018-09-12 09:59:44 | 25333 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study has been carried out in waters of Bushehr province in the Persian Gulf covering Nayband bay to Bahregansar between 26° 45 N to 30° 00 N and 49° 45 E to 52° 45 E, 15 main and 5 sub- stations, by R/V Ferdows 1 in winter 2004, summer 2004 and winter 2005. The physico - chemical parameters, nutrients (silicate, phosphate, nitrate and nitrite), phyto, zooplanktons, sediments and macrobenthoses were collected from water layers and sea bottom, and then analysed. The parameters were compared and evaluated with the results of the previous work, conducted in the same area. Termocline, halocline, oxycline and picnocline layers were formed in summer. These clins disappeared due to water column mixing in winter. The values of temperature, conductivity and pH in summer were higher than those of in winter, while the salinity and density values of surface and subsurface layers in winter were higher than those of in summer. The temperature and conductivity have been decreased from surface to depth, from coastal rigions to offshore and from southern waters to northwern waters, while salinity, density and turbidity have been increased. The dissolved oxygen is decreased by increasing the values of temperature, salinity and depth and it is observed that D.O. has increased in correlation with chlorophyl a in mean and subsurface layers. The pH was rather decreased from surface to depth while turbidity was increased. Orthosilicate (Si_SiO44), nitrate (N_NO3), and nitrite (N_NO2 ) concentration have increased from surface to depth while orthophostate (P_PO43) decreased. Among the phytoplanktons, Dinophycea had most abundance in winter 2004 and Diatoms had most abundance, variety and distribution in summer 2004 and winter 2005. Among the zooplanktons, Copeopda had most abundance, variety and distribution. The intensity of phytoplanktons in summer was more than those in winter; it decreased from surface to depth and from coastal rigions to offshore, at first increased and then gradually decreases. Intensity of zooplanktons in summer was more than those in winter and decreased from surface layers to depth and from coastal waters to offshore. The abundance of benthoses in summer was more than that in winter while their biomass in winter was more than that in summer. The abundance decreased from southern to northwern waters, but the rate of decrease in biomass was more than that of abundance. The mainly texture of sediments was loamy or muddy and the grain size in southeastern and northwestern areas were coarser than those in middle area. Compared with the previous study, the trend of physico chemical, silicate, nitrate and nitrite were the same but in spite of the past, the values of pH in summer were more than those in winter and also phosphate concentration had been decreased frome surface layer to depth. The overall trends of phyto and zooplanktons densities are almost similar to previous observation, but cyanophyceae significantly decreased, in comparison with spring and summer abundance. It is also observed that the zooplanktons average density has been in reverse order compared to previous observations. Average concentration of phosphate in the entire region was seven times more than that of the former study and its maximum value has rised more than 26 times in the petroleum and gas industries areas. On the other hand, the abundance and diversity of phyto and zooplanktons and also the ratio of biomass to abundance of benthoses were decreased.
    Keywords: Ecology ; Iran ; Persian Gulf ; Bushehr Region ; Nayband ; Bahregansar ; Hydrology ; Hydrobiology ; Nutrients ; Phytoplankton ; Zooplankton ; Sediments ; Macrobenthose ; Temperature ; Dissolved oxygen ; Chlorophyll a ; pH ; Diatoms ; Copeopda ; Depth ; Monitoring
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  • 6
    Publication Date: 2021-07-16
    Description: The culture of three species green tiger prown (Penaeus semisulcatus) ,white leg shrimp (Litopenaeus vannamei) and Indian white shrimp (Fenneropenaeus indicus) were grown in 7 ponds (0.4 and 0.25 hectare) for 110 days at a stocking density of 25 shrimp/m2 on Hele Research Station. The average of temperature and salinity was 30 degrees centigrade and 46-48 ppt .At the end of the culture mean weights of L.vannamei 18.68±2.6 was higer than the P.semisucatus 7.77±1.49 and F.indicus 14.66 ±2.1 also results demonstrated that there was a significant relation (P〈0.05) between mean weight. Final yield were 3114, 1983 and 848 Kg for L.vannamei, F.indicus and P.semisucatus.The FCR was better for L.vannamei (1.28) than F.indicus (1.48) and P.semisucatus (2.15).
    Keywords: Aquaculture ; Iran ; L.vanname ; F.indicus ; P.semisucatus ; Mean Weight ; Culture ; Native species ; Green tiger prown ; Penaeus semisulcatus ; Shrimp ; Indian white shrimp ; Temperature ; Salinity ; FCR
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  • 7
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25352 | 18721 | 2018-09-14 06:57:48 | 25352 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: 15000 juvenile Indian majour carps (Labeo Rohita,Cirhinus merigala, Catla catla) imported (200 ) and transferred to the earthen ponds in Astaneh Fisheries Research Station , In order to assess the viability of rearing under the climatic condition of north part of Iran and also achieve some growth parameters from the larval stage to market size.The fry with 300 mg weight, were released in to 12 ponds. Specimens were separated to tree experimental categories: 1= (30%indian carp and 70% chinees carp) , 2= (50%indian carp and 50% chinees carp) , 3= (70%indian carp and 30% chinees carp) .single chinees carp production were used as control . fishes were fed with artificial food and also with the natural products of the ponds which were enriched with organic fertilizers, after being equally stocked in the ponds .During the experiment, oxygen level ,PH, turbidity, and temperature were measured. Monthly fry samples were taken, for weight and length biometrical analysis and also determination of the weight gain percentage, average daily growth rate, specific growth rate. Results have indicated adaptations and considerable growth in this species, with an increase from the initial weight of 300 mg to an average of 13.5±1.6 mg in 50 days period until the end of November for fingerlings. Also the average weight of growing phase (second year of production ) was 647g for Labeo Rohita ,420 g. for Cirhinus merigala and 734g for Catla catla. The special growth rate (SGR)in Roho was 4.51 %.and 3.9 % for merigal and6.2 % for Catla while for Chinees carps SGR was 4.9, 6.7, 7.6 and 5.8 for silver carp,grass carp, common carp and big head respectively. Results revealed that the Indian majour carps compatibility with the condition in Gilan province was succesfull and the combination of species and optimization of their compatibility were remind for futhre studies.
    Keywords: Aquaculture ; Iran ; Guilan Province ; Astaneh ; Indian carp ; Growth ; Labeo Rohita ; Cirhinus merigala ; Catla catla ; Polyculture ; Juvenile ; Earthen ponds ; Fisheries ; Rearing ; Larval stage ; Fry ; Specimens ; Artificial food ; Fertilizers ; pH ; Turbidity ; Temperature
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  • 8
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25400 | 18721 | 2018-09-20 12:09:36 | 25400 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The project carried out at Shrimp Research station of Bandargah in 2011 to determine the production parameters such as; Average body weight, production, survival and feed conversion ratio in closed- recirculating aquaculture system for shrimp culture. This project was performed in 4 m3 fiberglass tanks. 3 tanks was chosen for the control and 3 were assigned to treatments. Considered Species in this treatment was white shrimp (Litopenaeus vannamei) that with a density of 60 pieces per square meter, and was reared for 90 days. Culture in control as usual and change water regularly, but the treatments did not change water during the period of culture. Water in the treatment tanks was going to turn the filter system and waste was removed by mechanical and coal filter embedded in the route, and after filtering was transferred to the wastewater tank. Wastewater was aerated in this tank and after 24 hours was pumped out and return to treatments tanks. In the final of project, mean weight, survival rate and production for control and treatment, was respectively, 18.27 ± 2.84 and 20.70 ± 1.47 g, 73.83 ± 4.14 and 77.17± 13.66 percent, and 0.81 ± 0.14 and 0.97 ± 0.23 kg/m^2. Statistical analysis results show that there are no significant differences found between treatments and control (P〉 0.05), although in all the above criteria, the relative improvement was achieved to compared of control. But the results of feed conversion ratio was 1.27 ± 0.16 for treatments that are significantly different (P 〈0.05) with controls (1.56 ± 0.23). This result showed that efficiency of the food was better in closed- recirculating shrimp culture system.
    Keywords: Aquaculture ; Iran ; Bandargah ; Shrimp ; Culture ; Litopenaeus vannamei ; Weight ; Survival rate ; Species ; Feeding
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  • 9
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25145 | 18721 | 2018-08-26 13:53:20 | 25145 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: A survey on Parasitic infections on endemic fishes of North of west Azarbaijan province were done during July 2001 Oct 2002. Altogether 267 specimens from 11 different fish species were examined. The fishes were collected from seven stations of Aras, Zangbar and Ghotor Rivers. The fishes were transported alive to the laboratory where they were meaursed and weighted. Then they were killed by cutting the spinal cord.. According to the results of this study 28 different parasites were revealed, 23 of them were detected to the species level and 5 of them to the genus level. The metazoans are as follows: The monogeneans, are Dactylogyrus extensus , D. chramuli, D. lenkorani, D. kendalanicus, Silurodiscoides siluri, Diplozoon megan and Gyrodoctilus sp. Two digeneans :Diplostomum spathaceum , Allocreadium ispoporum. Three Cestods: Ligula intestinalis, Digrama sp. and Caryophylleous laticeps. Two nematods: Rhabdochona hellichi, Eustrongylides excisus and only one acanthocephalus, Neoechinorhynchus rutili . The Protozoans are: Ichtyophthirius multifilis, Trichodina perforata, Chilodonella sp., Amphileptus branchiarum, Apiosoma sp/. Vorticella sp/. Myxobolus musayevi, M. cristatus. And two protozans from freshwater fishes: Stylonchia pustulata. and Arcella vulgaris. According the results of this study five new parasites and six new hosts for Iranian. Freshwater fishes were introduced. Diplozoon megan from: Abramis brama, Carassius carassius, Capoeta capoeta, Cyprinus carpio, Rutilus rutilus and Alburnoides bipunctatus. Trichodina perforata from Lucioperca lucioperca, Capoeta capoeta, Alburnoides bipunctatus, Barbus capito and Silurus glanis. Myxobolus cristatus, Tetrahymena pyriformis and Amphileptus branchiarum from Capoeta capoeta. Finally , Stylonchia pustulata and Arcella vulgaris for the first time were recoreded from freshwaters of Iran.
    Keywords: Health ; Iran ; West Azerbaijan province ; Aras River ; Zangbar ; Ghotor River ; Survey ; Specimens ; Species ; Genus ; Parasite ; Dactylogyrus extensus ; D. chramuli ; D. lenkorani ; D. kendalanicus ; Silurodiscoides siluri ; Diplozoon megan ; Gyrodoctilus sp. ; Allocreadium ispoporum ; Digrama sp. ; Rhabdochona hellichi ; Eustrongylides excisus
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  • 10
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25150 | 18721 | 2018-08-26 16:58:21 | 25150 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This Research has been done monthly in Sefidroud River and its tributaries from Agust 2003 to July 2004. From the total number of 2027 specimens of caught fish, 800 and 629 were males and females respectively, and 598 specimens were undistinguished. Samples were caught by cast nets. Fork length range of all specimen was 33-255 mm (119.56 ±46) and their weight rang was 0.42-257.5 gr (34.4 ±39.06) gr and their age range was 0-5 (1.76 ±0.959) year. The average of relative frequency in C.c.gracilis was 65.1 % . The average of condition factor was 1.07±0.13. Between Fork length and weight of C.c.gracilis. Was exponential relation and coefficient of correlation was estimated 99.11%. Growth cofficient (k) was 0.114 ; L_∞ was estimated 373.5 mm. C.c.g consumed of Chrysophyta phylum more than other Phytoplankton phyla in Sefidroud river and from Bentic point of view, consumed of clay, Filamentous algae detritus, insects, seeds. Absolute fecundity range was 1823-9274 (4553.8 ±2169.1) eggs. Considering the GSI and frequency percent of maturity stages, spawning period of the fish begins from April and peak of spawning is in June and terminates in July.
    Keywords: Biology ; Iran ; Sefidroud River ; Survey ; Capoeta capoeta gracilis ; Aquaculturize ; Species ; Specimens ; Males ; Females ; Fish ; Samples ; Weight ; C.c.gracilis ; Growth ; Chrysophyta phylum ; Phytoplankton phyla ; Filamentous algae detritus ; GSI ; Maturity ; Spawning
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  • 11
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25423 | 18721 | 2018-09-23 16:29:10 | 25423 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The Blooming due to the some species of phytoplanktons especially Dynoflagellates has made some problems for water ecosystems and aquaculture. In this study, the density of phytoplanktons specially Cochlodinium sp. and also environmental factors such as temperature, pH, dissolved Oxygen, and transparency were recorded two weekly in 18 stations of Hormozgan province, Iran in order to monitoring of the possibility of phytoplankton blooming. During six months monitoring, the target phytoplankton, Cochlodinium sp was not observed in shrimp farms. But, other phytoplanktons and zooplanktons were observed as follow: 13 genus of phytoplankton and six genuses of zooplanktons has found in ponds, main water channel and sea. The diatoms with 10 genuses had the highest abundance and Dynoflagellates with 3 genuses had the lowest abundance and blue-green phytoplankton with one genus was in lowest group. Totally, diatoms with 77%, Dynoflagellate with 15% and blue-green alga with 8% abundance were the main populations of planktons in the studied area.
    Keywords: Aquaculture ; Iran ; Hormozgan Province ; Tiab ; Algae Cochlodinium sp ; Shrimp farms ; Species ; Phytoplanktons ; Dynoflagellates ; Aquaculture ; Temperature ; pH ; Dissolved oxygen ; Transparency ; Zooplanktons ; Blue-green algae
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  • 12
    Publication Date: 2021-07-16
    Description: The survey effect of salinity, turbidity and dissolved oxygen performed on Rutilus frisii Kutum juveniles with aim, which was determined about survival rate and histological changes in gill and kidney tissues. Juveniles provided from the Rajai's fish propagation center and then examined in Caspian Sea Ecology Research Center laboratory. The juveniles were investigated on three weight group (200-〈400, 400-〈600, 600-〈1000 mg) and two dissolved oxygen levels (7.6 ± 0.19, 3.8 ± 0.15 mg/L)in fresh water and Caspian sea water(12.5 ppt). Also, six turbidity levels (50 FTU, 430FTU, 2600 FTU, 7800 FTU, 15600 FTU) were probed in freshwater during 168hr. Each treatment has three replications. The factorial test evaluated for survival rate in treatments. The results shown that there was a significant difference among treatments in the different levels of factors, (p〈0.05, Duncan test). The juveniles' survival rate has decreased than control treatment in brackish water and turbidity in freshwater. It was demonstrated that with increasing fish weight, therefore, the survival rate is increased. The lowest survival rates under salinity stress (〉75%) had shown in 400-〈600mg weight group with dissolve oxygen: 3.8±0.15SE mg/l. In addition, the lowest survival rates under turbidity stress (95.83%) had shown that in weight group less than 400 mg with turbidity 15600 FTU. Therefore, survival of juveniles was high. However, gill tissue of juveniles under turbidity and salinity stress became short (necrosis) and thick (edema and hyperplasia) in the end of experiments. The juveniles gill tissues were not changes in the river clear freshwater. Therefore, the factors of gill tissue deformation were salinity and suspended particles in the water. However, the structures of kidney in the different groups of juveniles were similar. Nevertheless, juveniles glomerular diameter increased with increasing weight (p〈0.05, Duncan test). Furthermore, there was a increasing the internal cavity of the proximal and distal tubules and decreasing of glomeruli in diameter in the transfer of juveniles from freshwater to brackish water. Although the survival rate of juveniles is acceptable in this study, but there was abnormal changes in the structure of them gills. It appears that this change will cause a disruption in trend of juvenile's growth.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Rutilus frisii Kutum ; Juvenile ; Salinity ; Turbidity ; Oxygen ; Survey ; Survival rate ; Gill tissue ; Freshwater ; Brackishwater
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  • 13
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25168 | 18721 | 2018-08-26 17:35:37 | 25168 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study, influence of modified atmosphere packaging on shelf life of trout (Oncorhynchus mykiss) (whole fish without visceral and without head and tail fish) stored in 4 to 6ºc was examined. Fish stored in MAP condition and control samples, in different time, were tested for spoilage chemical factors (TVN, PV and pH), microbial parameters (total viable count, clostridium botulinum) and sensory factors too. Mixed gases including co2 (30-50%), N2 (40-65%) and o2 (0 to 20%) were used for trout (without head and tail =6 treatments) and (whole fish without visceral and control = 2 treatments) statistical the analysis results showed that examined factors were significant difference during storage (P〈0.001). Mixed gases haven t had inhibitory effect on spoilage factors (chemical and microbial parameters). However spoilage process was delayed. Increasing of chemical and microbial changes in control samples was higher than treatment samples especially TVN. The results also showed that shelf life of control samples stored 4-6ºc were between 6-12 days but in MAP samples were 19 days. Mixed gases including CO2 (40%), N2 (55%) and O2 (5%) were the best formula and the shelf life of fish (without head and tail) was 16 days where it was 19 days in whole fish (Lack of visceral). The results showed that storage of trout in MAP condition facilities storage and increasing of fish shelf life too.
    Keywords: Fisheries ; Iran ; Oncorhynchus mykiss ; Modified Atmosphere Packaging ; Shelflife ; Rainbow trout ; Samples ; pH ; Microbial parameters ; CO2 ; N2 ; O2
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  • 14
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25171 | 18721 | 2018-09-03 17:06:33 | 25171 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Larvae of the chironomid midges (blood worms) are known as use full live food for cultured aquatics. There are endemic blood worms in sediments of the earthponds of Bafgh area at center of Iran. This study tended to identification of two chironomid species, Chironomus aprilinus Meigen, 1830 and Dicrotendipes sudanicus Freeman, 1959 & 1961, from Iran as a new record. Culture of blood worms was successful in glass thanks with 16h. Light, at 24-26°C temperature. Eggs were incubated after 14 days. Suitable concentration of harvesting is 1-2 egg masses (1250eggs/m2). Enrichment of earth ponds or plastic thanks including sedime is offered for culture of chironomid larvae. Collectors prepared with low cost materials are useful for collection of egg masses. May, June and July are the best time for egg collection. Large blood worms are mainly available in winter. Results of body analysis of the larvae indicated high dry matter with good quality.
    Keywords: Aquaculture ; Iran ; Bafgh area ; Chironomid ; Identification ; Culture ; Collector ; Brackish water ; Chironomidae ; Aquatic ; Larvae ; Blood worm ; Sediments ; Species ; Chironomus aprilinus ; Chironomus aprilinus ; Feed ; Feeding
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  • 15
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25172 | 18721 | 2018-09-03 14:19:51 | 25172 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Abbarik River is an important river in Kerman. Study of limnology of Abbarik River for aquatics & culture was started from Mehr 1381 to Day 1382. Base of this study was physical- chemical and Biological factors. In this research 3 stations was appointed. PH- oxygen- salinity- Ca- Mg- Na- K- Cl and was majored and bentetic- fish was recognized. This study was achievement in 4 season and twice in month. Bentosis recognize was Ephemeroptera- Diptera- Anisoptera- Cleoptera Trichoptera Fishes recognize was Capoeta damascina- Nemachilus sargadensis- Cyprinion watsoni.
    Keywords: Limnology ; Iran ; Kerman Province ; Abbarik River ; Limnology ; Bentosis fish ; Aquatic ; Culture ; PH ; Salinity ; Oxygen ; Ephemeroptera ; Diptera ; Anisoptera ; Cleoptera Trichoptera ; Capoeta damascina ; Nemachilus sargadensis ; Cyprinion watsoni
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  • 16
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25174 | 18721 | 2018-09-03 17:15:43 | 25174 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The population genetic structure of five Caspian Sea sturgeon species was investigated. Totally 1121 samples of caudal and unault's fin tissue of the sturgeons (Acipenser persicus, A. gueldenstaedtii, A. stellatus, A. nudiventris and Huso huso) were collected from the Volga River (Russia), Ural River (Kazakhstan), Kura River (Azerbaijan), Sepidrud River and the coastline of the south Caspian in the Iranian waters as well as from the sampling stations selected for the marine survey for sturgeon stock assessment in the Caspian Sea. All samples were stored in 96% ethyl alcohol and transferred to the genetic laboratory of the International Sturgeon Research Institute. Genomic DNA was extracted using phenol-chloroform method. The quality and quantity of DNA was assessed by Agarose gel (1%) electrophoresis and spectrophotometry. The population genetic structure of Ship and Persian sturgeon was studied using both PCR-RFLP (D-loop and ND5/6 gene) and microsatellite technique and that of H. huso, A. stellatus and A. persicus were studied using microsatellite technique. After amplification of genes using PCR, the RFLP technique was used to digest mtDNA using restriction enzyme. The PCR products were electrophoresed on 6% sequencing polyacrylamide gels followed by silver nitrate staining. Data for PCR-RFLP were analyzed using REAP program and those from microsatellite technique were analyzed using Gene Alex. Population genetic parameters including allele frequency, expected and observed heterozygosity, effective allele, Shannon's index were determined. Genetic identity and distance were calculated following Nei criteria and Hardy Weinberg equilibrium was tested based on X2 and analysis of molecular variance (AMOVA) using Reap and Gen Alex at 99% confidence limit. Phylogenetic relationship was determined and drawn using TFPGA program. The population genetic structure and genetic diversity of the 1121 sturgeon specimens were determined. Three independent populations were identified for Acipenser persicus (two populations in the south Caspian in the Iranian waters and one in the north Caspian). Three independent populations were identified for A. gueldenstaedtii (Volga, Ural and South Caspian populations) using the microsatellite technique. Population genetic structure using PCR-RFLP revealed no genetic differentiation among the A. gueldenstaedtii specimens studied from the different regions using ND5/6 gene, while two populations (Ural and south Caspian populations) were detected for this species with the same technique using D-loop genes. Four independent populations (Volga, Ural, Kura and Sepidrud populations) were reported for A. stellatus using the microsatellite technique and four more populations which most probably belong to the autumn and spring races of the above mentioned independent populations were identified for this species. The present study also identified two populations for H. huso; The North Caspian population (in Volga and Ural Rivers) and The South Caspian population (in Golestan and Guilan regions) which were significantly different from each other (P〈0.01). The genetic population structure of A. nudiventris was studied using the microsatellite and PCR-RFLP techniques which revealed two populations for this species one in the Ural River and the other in the Sepidrud River (South Caspian). Comparison of the ND5/6 and D-loop genes studies in Russian sturgeon revealed that the D-loop gene is better than the ND5/6 genes in population's differentiation and is therefore strongly recommended for population genetic studies on sturgeons in the Caspian Sea. Genetic diversity studied using microsatellite technique was higher and more accurate as compared to that using RFLP. Nevertheless the RFLP technique was able to introduce molecular markers for the population’s species pacific identification. On developing suitable primers these studies can be speeded up and the cost of such studies can be cut down. However the drawback in using microsatellite technique for population genetic studies is that it cannot introduce a molecular marker for the identification of populations. The present study was able to introduce molecular markers to differentiate the ship sturgeon population in the south Caspian from that in the Ural River using the PCR-RFLP technique Based on the results obtained it is strongly recommended that all activities related to restocking and rehabilitation of sturgeon stocks in Iran be conducted on the basis of genetic principles. Also serious and immediate measures should be taken for the restoration and conservation of rare population of native species of Iran particularly in the Sepidrud region using genetic markers before they are become extinct.
    Keywords: Biology ; Iran ; Caspian Sea ; Guilan Province ; Golestan province ; Sepidrud River ; Assessment ; Sturgeons ; Population ; Genetic ; PCR-RFLP ; Microsatellite ; Species ; Samples ; Tissue ; Acipenser persicus ; A. gueldenstaedtii ; A. stellatus ; A. nudiventris ; Huso huso ; Survey
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  • 17
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25179 | 18721 | 2018-09-03 17:23:17 | 25179 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study two species of algae, Sargassum glaucescens and Padina boergeseni that found plenteously in Persian Gulf and Bushehr coast, were collected and hot water extracts of them were lyophilized. F. indicus (11.32±1.20 g), after two weeks adaptation in Shoghab research station were immersed in seawater (39 ppt and 25±1 ○C) containing hot-water extract of each brown algae, S.glaucescens and P.boergeseni, at 100, 300 and 500 mg/l concentration, Survival rate and immunological parameters (total haemocyte count (THC), total plasma protein (TPP), Phagocytic activity, bacterial clearance efficiency and bactericidal activity) were examined. In addition effect of dietary administration of beta 1, 3 1, 6 glucan on prevention of White Spot Disease and immunological parameters of shrimp were investigated. According to results, immersion in seawater containing 300 and 500 mg/l concentration of algal hot-water extract after 2 and 3 hours or oral administration of beta 1,3 1,6 glucan at level of 10 g/kg diet for 14 days significantly enhanced THC, TPP, Phagocytic activity, bacterial clearance efficiency and bactericidal activity. Immersion in seawater containing 100, 300 and 500 mg/l hot-water extract of S.glaucescens after 3 hours, improved the survival rate of WSSV-infected F. indicus.
    Keywords: Biology ; Chemistry ; Iran ; Persian Gulf ; Bushehr Province ; Beta-glucan ; Sargassum ; Padina algae ; WSD ; Fenneropenaeus indicus ; Species ; Algae ; Brown Algae ; S.glaucescens ; P.boergeseni ; Survival rate ; White Spot Disease ; Shrimp
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  • 18
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25520 | 18721 | 2018-10-05 07:57:39 | 25520 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this survey, hydrology and hydrobiologycal studies in the northern part of the Oman Sea and Strait of Hormuz within the Iranian waters were conducted during 2007 and 2009.The project was implemented using Ferdous research vessel.The sampling area included the whole stretch of the Iranian waters along the northern part of the Oman Sea from the northeast of the Iranian marine border of 30 miles Bay of Chabahar to the mouth of the Hormuz Strait in Hormuzgan province. Along this stretch 10 equally distributed transects at a distance of 30 miles away from each other were designated, 4 main sampling stations at a distance of 10 miles from one another were fixed at each transect. Sampling was conducted twice a year (spring and autumn 2007 and 2009). According to achieved results; we can point out to the following outlines below: permanent thermocline exists annually in this body of water; where only the point of start, and the fracture width of thermocline layer; alter with alteration of seasons. Horizontal and vertical distribution of electrical conductivities, obey exactly from thermal degree structure.Level of salinity increase from east to west, but it decrease; from surface to deeper layers. In deeper regions, especially in the midway east of the Oman Sea; the level of salinity in the deeper waters from 150-300m, there is a considerable increase observed in salinity, due to very high salinity waters of The Persian Gulf; that are entering the Sea of Oman, via the Striate of Hormouz from the bottom; where they are having their effect following the whole length of the Sea of Oman; through their path. The level of water density from offshore to onshore, and from surface to deeper levels increase in such a way that. The level of Chlorophyll-a in surface waters, in the northeastern midway; is higher than the northwestern midway of the Sea of Oman, and it decreases from inshore to offshore waters, but its vertical distribution; has caused somehow in a way, that the highest distribution and concentration of chlorophyll-a; to be formed in deeper layers, between about 10-40m depths. Then by the increase in water depth, its concentration decreases drastically; reaching zero in magnitude. Concentration of dissolved oxygen is higher in spring in comparison to that of autumn, and the highest level is achieved in water layers located at 10-40m depth (where the level of chlorophyll-a is higher). In addition to which, that its vertical structure; shows the existence of a permanent oxycline layer at this region, in a way that; by seasonal alterations (similar to that of thermocline layer), only the point of start and that of the thickness of oxycline layer changes.pH level decreased from water surface, and its vertical alteration trend; obeyed to that of the same for temperature and chlorophyll-a vertical structure, especially for dissolved oxygen parameter, where the maximum decrease was recorded coinciding with formation of oxycline layer.Nutrient levels increased; at surface water layers from offshore to inshore, and from west to east, for the same body of water. In addition to that, concentration of nutrients in autumn (after monsoon); were more than that of, spring season (before monsoon), where; concentration of their vertical structure increase from water surface toward deeper sections. In this survey six phylum of phytoplankton including in Bacillariophyta (88) Pyrophyta (111), Cyanophyta (6), Chropmophyta (2), Euglenaphyta and silicoflagellate (1) were identified. A result showed that density decreased in most transects from inshore toward sea and maximum density was in photic layer (0-25m). Phytoplankton densities (without Cochlodinium density) in post-monsoon were recorded higher than pre-monsoon, furthermore phytoplankton density in 2009 was more than 2007(6073±1038) Species richness was indexed in the pre-monsoon and post-monsoon 2007 (0.337-0.519), (0.296-0.396) respectively and to (0.967-1.525), (1.407-1.531) for 2009. Zooplankton population in this study is characterized by eight phyla, eight classes, 15 orders, 35 families and 78 genus. In 2007, the pre-monsoon percentage of groups were frequently as following: Copepoda with stages of Nauplius and Copepeditide were 78%, Ciliphora 8%, Sarcomastigophora 4% and Hemichordata 6%. In post-monsoon order of frequency Copepoda 80%, Ciliophora 6%, Coelenterata 5%. Copepoda was the most abundant group of zooplankton.In 2009, The pre-monsoon Copepoda (82%), Cilillophora(9%), Sacromastigophora(4%) and Hemichordata (2%) in post-monsoon Copepoda(70%), Ciliophora (20%),Sarcomastigophora(4%) and Hemichordat (2%) dominante group were respectively. The static result showed significance betweendifferent transects and layers.We were recorded 31 ichthyoplankton families with different density and distribution during 2 years. Among benthic invertebrate polychaete with average 800 ind-2 and then amphipods and gastropods were the most abundance.Yearly density of macrobenthos showed polychaete with the average of 500 ind.-2 in 2007 and 1000 ind.-2 in 2009 was the most abundance . Macrobenthos density in postmonsoon was more than premonsoon.p〈0.05. An increasing and decreasing in density and biodiversity was obtain from coast to depth respectively p〈0.05.There was no significant different between macrobenthos density from Hormoz strait to Golf of Guiter.p〉0.05.There was a negative regression between macrobentos density and depth(p〈0.05) and positive rsgresion between macrobenthos and oxygen concentration and temperature.(p〈0.05).There was also a significant relationship between macrobenthos ,coppepda and polychaete density with organic carbon.p〈0.05.Bimass changed from strait to Golf and coast to depth increasingly.p〈0.05. The results of the measurement of heavy metals in water and Sea bed showed that the Concentration of heavy metals in surface layers in waters of the northern half of Oman Sea and Strait of Hormuz in spring (pre-monsoon) is more than autumn (after monsoon), and Degree of pollution in these areas are low compared to the global scale, Also, The results of the degree of contamination of sediments across the northern half of Oman Sea and comparison Global indicators of environmental quality assessment, it was found that the degree of contamination in the area in spring and autumn are low pollution. Comparison of the TPAH concentration of in Iranian waters of the Gulf of Oman, with other parts of the world indicate that degree of pollution of the waters in the spring 86 in the other seasons too low to medium level is low; but the degree of contamination in sediments, both in spring and in the autumn is much less; It means that the current risk on behalf of these contaminants, does not threaten the fish and benthos. The results of the risk assessment (THQ) of heavy metals in consumer, for yields Sangsar fish and fish Gish showed that the risk for both all the heavy metals, significantly lower than the one(1〈) and This means that urban communities in the province does not present any danger of feeling threatened.
    Keywords: Ecology ; Iran ; Oman Sea ; Strait of Hormuz ; Bay of Chabahar ; Hydrologi ; Sampling ; Chlorophyll-a ; pH ; Nutrient ; Bacillariophyta ; Pyrophyta ; Cyanophyta ; Chropmophyta ; Euglenaphyta ; Silicoflagellate ; Phytoplankton ; Copepeditide ; Ciliphora ; Sarcomastigophora ; Hemichordata ; Copepoda ; Ciliophora ; Coelenterata ; Zooplankton ; Polychaete ; Macrobenthos
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  • 19
    Publication Date: 2021-07-16
    Description: Survey is conducted to evaluate the effects of different intensities and durations of red light spectrums on growth performance and survival of Caspian trout (Salmo trutta caspius) in Coldwater Fishes Research Center (CFRC). According to the standard methods and manuals, selected 1.1±0.2 g (1755 individuals) larvae transferred from a registered and well-known hatchery and introduced to the fish tanks in CFRC after quarantine and adaptation procedures. The trial designed for 9 treatments with 3 repeats and fish tanks (California troughs) stocked by 65 larvae. All tanks were separated by dark plastic sheets individually to more controlling actions on special lighting regimes, except controls. Lighting actions were conducted as long as survey stage for 36 days by economic lamps (P: 40/50 W, =550/880 lum) on lighting durations of 12/12, 10/14, 14/10 h (on/off) and 24 (on) by 40/50 W lamps respectively for treatment 1 to 8. Controls are considered in treatment No. 9 by natural and environmental lighting regime. The biometry operations were carried out in a 6 days interval. Results were analyzed with SPSS (Version 12) software and by ANOVA one way analysis method and multiple levels of Duncan Test; graphs were illustrated by Excel software. According to the results, most of the measured factors as growth indicators (FW, WG, FCR and SGR) in treatments (1-8) had showed significant differences in compare of control. In this case, oily drips in retina layer of eye s larvae absorb the short light spectrums and let the long spectrums to be transferred. Thus because of more vision, feeding behavior could develop with lower stress condition. Moreover, some growth factor (SGR, WG and FCR) rates in larvae showed better values significantly in treatment (1) (12/12 h lighting with 550 lum) in compare of other treatments and controls (P≤ 0.05). In addition, there are not any harmonized and expected growth parameters with synchronized red lighting intensities and durations in the Caspian trout larvae. It seems that recent demonstrations were influenced by physiological effects of some endocrinal glands and brain neurotransmitters.
    Keywords: Aquaculture ; Iran ; Caspian trout ; Salmo trutta caspius ; Larvae ; Red light ; Light strengthen ; Light duration ; Growth ; Survey ; Survival ; Coldwater ; Feeding
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  • 20
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25563 | 18721 | 2018-10-05 16:51:28 | 25563 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study, the characteristics of physico-chemical parameters of water done in coastal part of the southern Caspian Sea during 2010-2011 (four seasons) in 8 transects (Astara, Anzali, Sefidrour, Tonkabon, Noshahr, Babolsar, Amirabad and Torkman). For measurement of water quality parameters, 480 samples were collected in different water layers by using of APHA standards methods. According to modeling and statistical methods was used multivariate for evaluation of factor analysis (MFA) in general groups and discriminant analysis (MDA) for spatial and temporal with water quality parameters. The statistical procedure of MFA was used for data reduction and finally six factors selected with about 80.48 percent of total variances related to them. The statistical procedures of MDA were used for the role of spatial and temporal of water quality parameters in different water layer. The function one gave eight parameters (pH, salinity, EC, DO, NO^3-, water temperature, NH^4+ and N^-total) affording more than 80, 67, 80, 77 and 71 % correct assignations (return to the same transects) in spatial analysis. In all transects during four seasons, function one gave five parameters (water temperature, salinity, EC, NO^2- and NH^4+) to afford 100, 100, 100, 97 and 97 % correct assignations in temporal analysis. The results showed that, the average amounts in light penetration layers during four seasons were for EC(15.04±0.18ms/cm), pH(8.38±0.01unit), salinity (10.31±0.16g/l), DO(5.67±0.09ml/l) and water temperature (18.28 ±0.72°C). The average amount of nutrients for parameters such as organic and inorganic phosphorus, total phosphorus, NO^2-, NO^3-, NH^4+, N^-Total and SiO_2 were 0.50±0.02, 0.32±0.01, 0.82±0.02, 1.76±0.10, 0.10±0.01, 1.51±0.10, 46.82±1.80 and 8.60±0.29µM, respectively. The average amounts in dark layers during four seasons were for EC (15.46±0.38ms/cm), pH (8.34±0.03unit), salinity (10.70±0.35g/l), DO (4.55±0.13ml/l) and water temperature (9.62 ±0.16°C). The average amount of nutrients for parameters such as organic and inorganic phosphorus, total phosphorus, NO^2-, NO^3-, NH^4+, N^-Total and SiO_2 were 0.54 ±0.05, 0.33 ±0.02, 0.87±0.06, 1.92±0.21 , 0.08± 0.01, 1.55±0.25, 45.10±3.25 and 10.05±0.62 µM, respectively. The average amounts of light penetration in all transects during four seasons 4.49±0.38 meters. The maximum light penetrations during spring, summer, fall and winter seasons were 9, 8, 8.20 and 7 meters, respectively. The maximum water temperatures in light and dark layers were 32.36 and 11.50°C. Also, the maximum differences about thermocline temperature during summer and fall seasons were 19.6°C and 10°C, respectively in southern part of Caspian Sea. Data in spatial analysis were not significant (p〉0.05), it means if add or removed any transect did not change occurred. But in temporal analysis were significant (p〈0.01), and it means no way remove any seasons for a research work on Caspian Sea in southern part. In those transects with 100m depth. Water temperature is only highly effective parameter between other parameters. In function 1 between 3 functions in transects with 100m depth water temperature with 60% variance was the main role and effective between different seasons in depth part were very high.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Astara ; Anzali ; Sefidrour ; Tonkabon ; Noshahr ; Babolsar ; Physico-chemical parameters ; Nutrient maters ; Sea water ; APHA ; pH ; Salinity ; Temperature ; Nutrients
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  • 21
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25200 | 18721 | 2018-09-03 17:38:28 | 25200 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Anzali wetland at south west of Caspian located along of North West and southeast and with its unique feature were add on the Ramsar convention list in 1975. There were recorded a high rich of fauna and flora including 276 plant species, 23 reptiles and amphibians, 60 fishes, 26 mammals and 195 birds. Anzali wetland were encountered with numerous disorder with the eutrophication and the early dismissing. The first studies on Anzali wetland started by many international institutions in 1964. In this study the data of biotic and abiotic factors in Anzali wetland analyzed which belongs to different sites (15 to 38 stations) during 1991 to 2000 and were sampled seasonally or monthly in different years. The results showed the increasing trend of phytoplankton abundance from 7.9 to 66 million C./l. and the western part had the most abundant. The Cyanophyta had the most development than other 7 phylum observed in the area. In Anzali wetland identified 143 phytoplankton genus that 9 genus dominated in more than 80 % replicates and 7 genus had the abundance of more than 5 million cell/l. The Zooplankton abundant increased from 580 to 2200 ind./l. The Rotatoria had the most abundant followed by Copepoda. There was identified 104 zooplankton genus that 5 genus were dominated in more than 80 % replicates and 11 genus had the abundance of more than 30 ind./L. The chlorophyll a varied from 8.8 to 50.2 μg/l. and had a increasing trend after 1996, meanwhile it was measured higher than 25 μg/l in most of the stations. The results of benthic study showed that the Chironomidae and Tubificidae were observed more than other organisms which comprised 12.2 to 23.5% of total biomass of benthic organisms in studied period. The range of biomass was 1.1 to 7.8 g/m2. The ichthyologic survey showed presence of 60 species that 11 species were exotic. 28 species belong to freshwater, 17 species were anadromous and 8 species were resident in estuary. The highest and lowest fish catch were 640 and 288 ton in 1994 and 1999 respectively and it was about 500 ton at the end of period. The hydrochemical factors had the various fluctuation so that the nutrients parameters had a significant difference over studied period. Total nitrogen was varied from 0.78 to 1.41 mg/l and total phosphorus varied from 0.05 to 0.1 μg/l.. The oxygen dissolved amount was from 4.6 to 8.7. EC was between 3 to 5 ms/cm during September and October that indicated of Caspian water projection. According to geographic position of station, all biotic and abiotic data, were arranged. The data bank is used by Idrisi software to produce of necessary maps. Analysis of data showed that there must be a responsible department with a vast authority. Conservation and eutrophication prevention of Anzali wetland connect to special attention to catchment area and landuse management of the wetland. Also there is an urgent need for well-developed technologies and managements to reduce the organic pollution and its environmental impacts on the Anzali Lagoon.
    Keywords: Limnology ; Iran ; Caspian Sea ; Anzali wetland ; Ramsar ; Anzali Lagoon ; Limnological ; Survey ; GIS ; Fauna ; Flora ; Cyanophyta ; Phylum ; Phytoplankton ; Zooplankton ; Chlorophyll a ; Biomass ; Species ; Hydrochemical ; Nutrients ; Dissolved oxygen
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25201 | 18721 | 2018-09-05 16:03:37 | 25201 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Use and enrichment of live food resource in fish farms have been interested and highly demanded. Crustacean are one of the important groups. The Pontogammarus maeoticus dominated in southern Caspian Sea shore with a high abundance. This study was designed in order to adaptation and usage of amphipoda in fish culture ponds. The first part have been surveyed the laboratory experiments including of; to increasing and developing of P.maeoticus in 200 litter container, the effects of salinity on growth and survival of amphipods in many aquariums, the culture of common carp with amphipods and growth determination of them. Chemical composition analysis of P.maeoticus and carps fed by amphipods in compare to cultured carps from ordinary ponds. Due to concern about common healthy the heavy metal concentration has been measured in P. maeoticus, carp which were fed by amphipods and the cultured carps in earth ponds. In second phase; the adaptation of two amphipods species, P. maeoticus and Obesogammarus acuminatus was studied in fish ponds where some cages with sandy soft substrate had been provided for amphipoda replacement. Also a small surface of ponds surrounded by net and covered by Azola plant, a habitat suitable for to putting of O. acuminatus. Production of amphipoda had not the successfully results in large tanks. Aquariums with Caspian Sea water had the prosper results where the specimens were breeding and developing properly, even though in some aquarium with freshwater increased the amphipods number. The chemical composition had not significant difference between two kind of cultured carps while the organic component in amphipoda had a high quality. The better quality of cultured carp by amphipod diet have been confirmed by organoleptic test. The results of heavy metal measurement in amphipoda showed a high concentration which some of them were transmitted to cultured carps. Result of amphipoda replacement in cage was not satisfy and the specimens were died after some days. According to hydro-chemical parameters the oxygen poorness and high trophy levels were the affective factor to abolish of specimens in cages. It seems that there are many type of P.maeoticus that can be adapted in different salinities. The molecular differentiation should be investigated to choose the suitable type of this spices for utilization in freshwater fish ponds. In other hand it can be used in fish culture ponds that will be supplied by brackish water.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Adaptation ; Amphipoda ; Fish ; Culture ; Ponds ; Enrichment ; Pontogammarus maeoticus ; Amphipoda ; Survey ; P.maeoticus ; Common carp ; O. acuminatus ; Oxygen ; Specimens ; Brackish water
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25206 | 18721 | 2018-09-04 08:43:13 | 25206 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: From June 2004 to March 2006, a survey in Iran waters (Bushehr province) has completed. Sampling was accomplished in two area, north and south. In this period 15 cruises achieved in summer and 3 cruises in autumn and winter. In each cruise of north and south area 18 and 30 stations were sampled respectively. Stations row on three depth stratums that is, below 10 m, 10-20 m, and 20-30 m in total area from Bahrekan to Motaf. Some Biological parameters such as sex stages, sex ratio, mean total length, species composition were measured. Duration of shrimp fishing season in years 2004, 2005 and 2006 were 40, 44 and 40 days in summer respectively. In June and august the Catch per Unit Effort (CPUE) in south was more than northern area. CPUE increases in north area especially at the end of summer during fishing season gradually. Computing on Biomass estimation resulted 674, 1024 and 873 ton in years 2004, 2005 and 2006 respectively for all shrimp species (usually tiger shrimp, white shrimp and other). Total commercial landing of shrimp catch in above years were 1602, 1597 and 1110 ton respectively. Percentage of tiger shrimp in commercial landing in above years was 77, 75 and 84 respectively. We found that the least mean length of tiger shrimp was observed in southern area especially from Motaf to Rodmond stations. The schools of prawn penaeus semisulcatus enter to Bushehr waters from southern area that is Motaf region. Other than Motaf; Nakhilu, Rase khan and Rod-mond show same characteristics. Also in this region, density of shrimp increases with depth in most summer cruises. The result from analyses of gonad maturation studies shows the adult and mature females (stages III and IV) of P. semisulcatus accumulate in northern area at the end of autumn. This stock catches in autumn by illegal fishing.
    Keywords: Fisheries ; Iran ; Persian Gulf ; Bushehr province ; Biomass ; Shrimp ; Penaeus semisulcatus ; Fishery ; Green tiger prawn ; Opening season ; Closure season ; Survey ; Sampling ; Sex ratio ; Species ; CPUE ; Illegal fishing ; Parameters
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25207 | 18721 | 2018-09-05 16:22:47 | 25207 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Increasing of biodiversity and reduction of production costs and alo increasing of shrimp production are the main elements of production security and continuation of aquaculture of shrimp in the country. The hypothesis of this study is made on the basis of aquaculture of pacific white legged prawn, litopenaeus vannamei, and that is economically better than Indian white prawn in Bushehr area. This study was carried out with the objectives of determination of bionormatives of pacific white legget prawn and yield estimation per square meter and has been compared with Indian white prawn. During 1384-85. In this study, the field operations were carried out after primary preparation and water enrichment with the juvenile prawn (pL 15) with 20 prawn per squar meter in the 3 ponds. During the study the phisico-chemical parameters such as water temperature, ocsysion, pH,two times per day , in the morning and evening and salinity , transpiration and depth water , only one time in a day were measured .The health of prawns and also sampling for the calculation of body weight were carried out in every 10 days . Feeding in the first month was randomly and after that was being continued based on the mean of the body weight. The results of this study have showed that the growth of this species is very fast so that during 90 days with the capacity of 20 prawns per squar , meter, it can be reached to 19.59 gr body weight with the growth rate of 0.217 gr . The mean of total production was 2737.5 kg per hectar with FCR=1. During this study the salinity from 33 ppt to 54 ppt, water temperature from 24.3°c to 35.2°c, occsysion from 2.4 to 11.89 mg/l , pH from 7.83 to 9.01 , transparency from 35 cm to 90 cm and water depth from 100cm to 145 cm were measured and recorded . In the second year, the comparison of growth rate of white legged prawn and Indian white prawn was carried out in the four ponds. The results of this study showed that during 110 days. White legged prawns was reached to average of body weight 14.66 gr and Indian white shrimp, reached to 10.75 gr. The economical assessment showed that white legged prawn has an economical advantage around 2.59 times during 110 days with the capacity of 35 prawns per hectar.
    Keywords: Aquaculture ; Iran ; Bushehr ; Western white legged shrimp ; Indian white shrimp ; Culture ; Bionormative ; Commercial production ; Aquaculture
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25618 | 18721 | 2018-10-07 16:34:53 | 25618 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Chitin and chitosan are 2 very important products of biopolymer that enjoy high consumption in industry, but their production sources are very limited. In this study, Artemia urmiana cyst shells were obtained from previously collected and stored ones in Iranian Artemia Research Center. 20 kg of Artemia urmiana cyst shells were sampled, cleaned, separated, dried and transferred to Iranian Artemia Research Center Laboratory to extract their Chitin and chitosan. Their chitin and chitosan initially were extracted using optimized common chemical methods. Their properties were compared to 2 other types of Chitin and chitosan obtained from crab and shrimp manufactured by Vietnam and China, respectively. To determine their quality, elemental analysis device, infrared spectrophotometry, x –ray radiography, determination of viscosity , molecular weight, crystallinity percent, color, de stylization measure, empirical and molecular formulas were made. The results showed that the percentage of chitin obtained from Artemia cyst Shells in Chemical method was 28 ± 3 % by weight and efficiency into chitosan (grade steel relief) in this method was 50± 5%. To optimize the extraction procedure and the removal of proteins of chitin by biological practices that were done by sodium hydroxide in the chemical method, it was replaced by the bacterium Bacillus subtilis. And in the bio- phase of chitosan de steelation fungus Aspergillus niger enzyme was replaced instead of sodium hydroxide at high temperatures. The results showed that chitin and chitosan can be extracted from Artemia cyst shell using biological method and their characteristics included as in chitin 49.6% C, 8.2 % N, 7.5 % H, and 34.5 %O. Also the same levels for chitosan were 44.4 %, 8.9, 7.2 and 39.5 %, respectively. Their other quality characteristics were included chitin average molecular weight 4.9×10^6 Dalton, crystallinity percentage of 36.4, viscosity at 20°C 31 centipoise and its color was gray to brown. In the biologic method, the average molecular weight of chitosan, crystallinity percentage, viscosity at 20°C, were 5.1×105 Dalton, 94.5, and 18 centipoises, respectively. Also, its color was pale brown. Chemical structure of extracted chitin and chitosan from the shell of Artemia urmiana cysts were C_7H_12NO_4 and C_6H_11 NO_4c, respectively. The comparison of chitin and chitosan obtained from each chemical and biological method revealed that replacing biological methods instead of chemical methods is possible in achieving these products at suitable condition and better quality. This can eliminate the use of chemicals damaging the environment such as sodium hydroxide and decrease environmental pollution.
    Keywords: Biology ; Chemistry ; Iran ; Artemia urmiana cyst shell ; Chitin ; Chitosan ; Chemical ; Biological methods ; Crab shell ; Shrimp shell ; Biotechnology
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25229 | 18721 | 2018-09-06 06:14:35 | 25229 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Study and recognition of the Quanat water of Rafsanjan, Sirjan and Ravar cities has been beginning since the November of 2004. Among 228 Quanat chains were studied in these cities, 165 quanat chains had a discharge more than 10 liter per second (L/Se) which were sampled. In this research oxygen, pH, discharge, water temperature and air temperature were measured in two times and in the local place. Height from the sea level, longitude and latitude were measured (by GPS Machine) in one time and in the local place and other physical and chemical factors including No_2, No_3, Co_2, total hardness, NH_3 and So_4 were measured one time in the laboratory. In this manner, the condition of connection ways of considered Quanat with city was studied. With examination on the Quanat water in above said cities, all of Quant was within desired extents for aquaculture activities. Moreover in some of them, water temperature was higher than 20 degree centigrade that with some changes and in specific months can be used in aquaculture.
    Keywords: Biology ; Iran ; Rafsanjan ; Sirjan ; Ravar ; Fish ; Aquaculture ; Quant ; Water ; Oxygen ; pH ; Temperature ; No2 ; No3 ; Co2 ; NH3
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25239 | 18721 | 2018-09-07 06:54:42 | 25239 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: To study the preparation of frozen minced meat from abramis brama orientalis commonly known as sim from Arras lake and from silver carp cultured in the Azerbaijan province, and later this minced meat converted to fish ball and fish burger, in this process microbial, chemical and organoleptic tests was done on samples for frozen minced meat on Sims fish the TVN was 10.1 in zero phase and 14.1 after three month and peroxide value from 0.9 t0 3.1 at the same duration. But he value was higher when converted to fish ball, TVN ranged from 14 to17.6 and per oxide from 1.1 to 4.7 after three month, the unit for TVN was mg/100gram and peroxide unit meq/1000 gram, the microbial tests was also resulted in an acceptable range, the meat of Sims fish had no significant difference with the silver carp comparatively only the color and the smell of the fish was little different, so we could conclude that we can use Sim fish meat for frozen minced meat product and this can increase the consumption of fish in the region in the future.
    Keywords: Fisheries ; Iran ; Azerbaijan province ; Aras lake ; Minced fish meat ; Frozen fish ball ; Abramis brama ; Silver Carp ; Culture ; Oreganoleptic test ; Samples
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25250 | 18721 | 2018-09-06 06:29:01 | 25250 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: An artificial Reef is a fabricated, underwater structure, typically built for promoting marine life in areas of generally featureless bottom. Creating artificial reefs began from 2000 in southern provinces in Iran. This study has surveyed about the Bushehr and Khozestan states. The objective is to determine the effectiveness of artificial reefs in attracting and enhancing the production different aquatics through increasing of fish assemblage and fishery. The performance of artificial reefs deployed off the coast of the Persian Gulf in 10 to 15m, was evaluated. The reefs comprised of seven artificial and one control (natural) statistical tests plan in three replicates. Three types of artificial reefs were used in this study. That two were designed reefs and one was not designed or used material. The experimental plan consisted (i) Reef ball (R), designed hemispherical shaped; (ii) Laneh Mahi (L), designed pyramid shaped; (iii) Used material (U); (iv) mixed (RL); (v) mixed (RU); (vi) mixed (LU); (vii) mixed (RLU); and (viii) control site (CS). Trap nets and under-water visual census surveys were adopted for seasonal sampling of fish aggregation. Results of statistical analysis using ANOVA and T-test of the mean Catch per Unit Effort (CPUE) showed significant difference (p〈0.05) in term of computing number of fish for aggregation of fishes. The study has concluded that reef deployments have influenced favorably the fish communities and fish harvests. Therefore, the artificial reefs, especially the mixed RLU, are appropriate tools for future fishing enhancement in the Persian Gulf of Iran.
    Keywords: Aquaculture ; Iran ; Persian Gulf ; Khozestan Province ; Bushehr Province ; Artificial reefs ; Fisheries ; Marine ; Survey ; Hemispherical ; ANOVA ; CPUE ; Underwater structure
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25245 | 18721 | 2018-09-07 07:22:16 | 25245 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Annually, many data of hydrology and hydrobiology of the southern Caspian Sea at Iran's coasts were collected by Caspian Sea Research Institute of Ecology. The data which had been collected by researchers submitted annually in several reports but has not been processed all at once. Fluctuations of various parameters were surveyed and the relationships between them were analyzed. The main goal of this research is to analysis the data were obtained from 1994 to 2006 and show changes the concentration of various parameters in the Caspian Sea. Physicochemical data indicated that the average trend of transparency and salinity was decreased from 1994 to 2006 annually, where the salinity value was decreased from 12.37 ppt (part per thousand) to 11.5 ppt. The average of dissolved oxygen was increased from 6.4 to 8.6mg/l. pH has slightly fluctuated from 8.15 to 8.31. However, with increasing depth from surface to bottom, the average of dissolved oxygen was sharply decreased (DO concentration in surface and 800 m was 7.18 and 1.45 mg/l, respectively). The trend of salinity and pH fluctuation was very slow with depth but the salinity has an increasing with incrementing of depth but pH has a decreased trend. The average of organic and inorganic phosphorus and TP was nearly increased. The average of Nitrate and Silica sharply increased comparing to depth but the average of Nitrite and organic Nitrogen decreased. The results showed that the temperature fluctuation in different water layers was low in winter but the dramatic decreasing of temperature was occurred at 10-20 m (in spring), 20-50 m (in winter) and 50-100 m (in autumn). With increasing of depth (Slope of the Sea), transparency and salinity values have an increasing while DO and pH showed decreased. As a whole, 335 species of phytoplankton were identified, there are 70 species in advance as these species were not seen in this period but 96 of novel's species have been recorded. The lowest and the most of phytoplankton biomass was recorded in 2006 and 2001 while the biomass was 59 and 1034 mg/m^3, respectively. Albet, the lowest biomass was recorded at the depth of 100 m (24.1 mg/m^3) but the maximum was observed in surface layer (1344.1 mg/m^3). The average of phytoplankton biomass was increased after arrival of the ctenophore. There was a significant difference between the average of biomass in different seasons before and after of the ctenophore invasion (P〈0.001). Bacillariophyta and Pyrrophyta have the most cell abundance with a total of biomass of 52.7 and 37, respectively. From 1994 to 2006, species diversity (Shanon-Niner), evenness and richness were estimated between 3.02-1.29, 0.28-0.61 and 4.32-7.60, respectively. A total of 65 zooplankton species were identified with a frequent species in the Caspian Sea before the ctenophore invasion, while the species diversity decreased after the invasion. The high and the lowest of species diversity was recorded in 1994-1995 and 2006 and in 1999 the biomass of the zooplankton were observed between 12.6 mg/l and 363.8 mg/l, respectively. After arrival of ctenophore, the averages of zooplankton biomass at of the different depths were sharply decreased and were less than the ctenophore invasion. The result showed that there was a significant difference among the average of zooplankton biomass in two periods, seasons and west, middle and east regions as follows: P〈0.001, P〈0.008 and P〈0.01. The maximum abundance and biomass of zooplankton belonged to copepoda with %51.5 and %63, respectively. Rotatoria falls in the second class from 1994 to 2006 (during 1994-2006), species diversity, evenness and richness were varied between 0.19-1.6, 0.08-0.52 and 0.37-2.29, respectively. The species diversity of macrobenthic organisms at the same period s followd by zooplankton changes which sharply decreased. The average of benthic biomass reduced from 13.7 g/m^2 in 1994-1995 to 1.8 g/m^2 in 2004 but sharply increased in 2005 with a maximum value of 46.7 g/m^2. With increasing of depth, the average of benthic biomass was sharply decreased. There was a significant difference (P〈0.001) in macrobethic organisms abundance before and after the ctenophore invasion, but it did not show a significant difference between seasons and different regions (as follows: P〉0.137, P〉0.782). Before the presence of ctenophore, the worms had been constituted a dominate group with an approximately %45 of total abundance and also %20 of their biomass. After this period, their frequency was sharply increased (more than %85) but the maximum value pertains to Cardidae (more than %90). The species diversity, evenness and richness were varied between .69-2.51, 0.23-0.63 and .011-3.79, respectively. AMBI software, Shanon-Viner parameter was moderate in all seasons from 1994 to 1996 but this parameter was reduced in a bad limit (boundary) in the most seasons from 2002 to 2005. The main parameter of M-AMBI consists of several parameters and also approximately showed similar changes such as Shanon -viner parameter. On basis of this parameter, the ecological quality condition of Caspian Sea was superior limit in all seasons from 1994 to 1996 but after the following years were decreased from good to moderate limits (After the presence of ctenophore). Relationship between abiotic variants and phytoplankton indicated that there was a direct relationship between transparency and salinity while there was a powerful and reversal significant relationship between transparency water temperature, transparency in organic Nitrate, phytoplankton number and transparency phytoplankton biomass. The comparison between different variants average in two periods (before and after the ctenophore invasion) indicated that the average of Kilka catch was decreased, the relative frequency of clupeonella engrauliformis and clupenoella grimmi was sharply reduced but the relative frequency of Clupeonella cultriventris was sharply increased, the species diversity, evenness and richness and the number of zooplankton species were sharply decreased, the average of biomass and transparency was sharply reduced, the average of dissolved oxygen and liza saliens or liza auratus catch were increased. These difference were often significant (P〈0.05). Therefore, with the ctenophore invasion into the Caspian Sea, the primary production was increased, the biomass value of zooplankton and specially Kilka which fed on zooplankton were sharply decreased while the fish such as Rutilus frisii kutum and mullet (Liza salins or Liza aurratus) which fed on benthic were increased.
    Keywords: Ecology ; Iran ; Caspian Sea ; Transparency ; Salinity ; Dissolved oxygen ; pH ; Nitrate ; Nitrite ; Species ; Phytoplankton ; Biomass ; Bacillariophyta ; Pyrrophyta ; Diversity ; Zooplankton ; Rutilus frisi kutum ; Liza salins ; Liza aurratus
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25256 | 18721 | 2018-09-07 07:21:26 | 25256 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Sampling was done using 90 post larvae which were produced by reproduction of some broodstock of Penaeus indicus in one day and reared in the same situation for 4 month. Samples were classified in 3 group high growth, medium and low (according to their weight and length). Genomic DNA was extracted from a 1cm^2 piece of muscle using the phenol-chloroform method. The polymerase chain reaction (PCR) was done using 21 RAPD loci. And PCR products were separated on 3% Agarose gels. From 21 studied loci, 12 produced polymorphic bands. The most polymorphic band produced using OPAQ 9 and the least by OPAQ 7. According to Nei 1972, the highest distance (0.457) was between low growth group and medium and the lowest (0.091) between high growth group and medium, therefore the highest identity (0.912) was between high growth group and medium and the lowest (0.633) between low growth group and medium. Consensus neighbour-joining tree using Nei (1972 and 1978) resulted in two clades, the first including high and medium growth groups and the second low growth group, it appears that low growth group are depended on separated population of the two others. With considering of mean weight of F1(16.25±1.5), mean weight of 15 ±1.2 and mean weight of parent 31.6, response to selection (R) and heritability for growth in this species were estimated 1.2±0.2 and 0.07±0.01 respectively. In another part of this study Sequencing of specific bands and primer design were done and examining of them on the same age specimens is necessary in following.
    Keywords: Biology ; Iran ; Penaeus indicus ; High growth ; RAPD marker ; Genetic ; Shrimp ; Breeding ; Culture ; Sampling ; Broodstock ; Phenol-chloroform methods ; Population ; Species ; Specimens
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25642 | 18721 | 2018-10-14 02:40:17 | 25642 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Padina boergesenii is one of the most abundant brown algae distributed in the north of Persian Gulf and Oman Sea. In this study after sampling and preparation of Padina boergesenii by Chroform-Etanol (3-1) solvent and by Methanol has been extract. Separation and purification of the compounds was carried out using thin layer, general and inverse column chromatography, Cephadex and high-performance liquid chromatography (HPLC). Structural elucidation of the constituents was based on the data obtained from H-NMR, 13C-NMR, HSQC, HMBC, DEPT and Cephadex LH-20. The steroids compounds separated from above alga were identified as 22dehydrocholesterol (1), cholesterol (2), fucosterol (3), β-sitosterol (4), stigmasterol (5), ostreasterol (6) and two epimer of hyroxyestrol(7), based on their spectral data and from comparison with those previously reported in the literature.
    Keywords: Biology ; Chemistry ; Iran ; Oman Sea ; Persian Gulf ; Brown Algae ; Padina boergesenii ; Steroids compounds ; Extraction ; Purification ; Identification ; Amount verification ; Sargassum glaucescens ; Algae ; Abundant ; Sampling ; Chromatography ; Cephadex ; Dehydrocholesterol ; Cholesterol ; Fucosterol ; β-sitosterol ; Stigmasterol ; Ostreasterol
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25792 | 18721 | 2018-10-13 08:54:26 | 25792 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Cheshmehkileh River and adjacent mountainous streams, play a strategic role as a historical axis for anthropogenic civilization, human welfare also habitat and migration pathway of commercial – biologic valuable fishes e.g. Caspian trout, Caspian kuttum, members of Cyprinidae family in south Caspian Sea drainage. Treats such as overfishing of Caspian trout and Red spotted trout stocks in mountainous headwaters, barriers construction and manipulations those are out of river carrying capacity developed by human activities, affected normal function of river as well. Sand mining big factories establishment next to the river, legal and illegal trade of river sediments, direct entry of Tonekabon landfill leakage into the river, development of Rainbow trout farms since 3 decades and huge effluents into the river containing dead fish and types of solids, escapement of cultured Rainbow trouts into the river, … are major minimum factors which needs basic information for integrating inclusively drainage management system. Cheshmehkileh River contains Headwaters of Dohezar (Daryasar & Nusha), Sehezar and Valamroud rivers during 13 monthly sampling phases between September 2009 and October 2010 based on macrozoobenthoses investigations by EPT, EPT/C EPA protocols, measurements of nominated physic-chemical and microbiologic parameters. Probability of Rainbow trouts escapement and invasion, existence, nutrition in Cheshmehkileh environment indeed investigated. Data analysis explained significant differences (P〈0.05) between groups of measured parameters in different sampling stations. Dendogram of clustered analysis based on consolidation of major biologic/ physic-chemical and microbiologic parameters, separated stations No. 1, 3, 2, 4 in one group and remained classified in different groups. Station 8 and 9 similarly separated which expressed general similarities according to Sehezar river environment which were differs in comparison with other stations. Station 11 separated according to its natural quality of water and environment. Similarities between station 10 to Sehezar river stations 8 and 9 expressed general influence of Sehezar River more than Dohezar River in Cheshmehkileh condition especially in station No. 10. High scores of EPT and EPT/C indices in upstream stations 1, 3 and 8 also low score of indices in stations 7, 13 and 6 expressed levels of environment quality between these groups of stations. Maximum average biomass of macroinvertebrates belongs to Trichoptera order in Cheshmehkileh River. Significant decrease of biomass in stations 11, 12 and 13 in comparison with other stations stated environment degradation in mentioned stations relevant to excessive sand mining as well. Pollution resistant groups of invertebrates significantly increased in downstreams against upstream stations. Also disappearing of Plecoptera order in station No. 7, 9, 10 and 13 stated low quality of environment in comparison with upstream stations. Confirmation of effects quality and quantity for point and non-point sources of imported pollutants require specific management considerations in order to present exploitations, pollutants control and emergencies for river monitoring in forthcoming years.
    Keywords: Aquaculture ; Management ; Iran ; Tonekabon ; Cheshmehkileh ; River ; Pollution ; Aquaculture ; EPTC ; Assessment ; Macroinvertebrates ; Chemistry ; Microbiology
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25833 | 18721 | 2018-10-13 10:28:58 | 25833 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study aimed to determine domain the adaptability of rainbow trout(Oncorhynchus mykiss) fingerlings in fresh water up to 20 per thousand (grams per liter) for providing facilities for growing this valuable species in the aquatic environment with salinity unconventional been completed. For this purpose the biochemical, bloody and physiologic parameters rainbow trout were studied to determine the adjustment range .Fingerlings fishes from cold water fish farm in the province Mazandran were prepared and for experiments were transferred to the Ecological Institute of Caspian Sea . Fingerlings with an average weight 31.56 ± 0. 07 SE g and average fork length 13.80 ± 0.15 SE cm, in 3 treatments in water with salinities (fresh, 13 and 20 grams per thousand) with a density of 15 numbers in polyethylene to 300-liter tank containing the 250 liters of water testing were introduced. Fresh water from Tajan rivers and water psu13 from the water Caspian Sea and water psu20 by mixing water the Caspian Sea and Sea salt was prepared . The daily amount to 50 percent of the of water tankss been replaced .During the experimental period was for 7 days and were not fed during the experiment.The water parameters was measured during the experiment included 6 ppm dissolved oxygen, pH equal to 8.2 and temperature 15.5 ° C . In the experimental period were not observed Losses in the experimental groups . The results showed that fish gill and kidney introduced in different salinities by making appropriate changes in chloride cells in the gills through increasing the number and the volume of these cells at the base of secondary blades and tubules in the kidney tubules to create greater interior space, are adapted to By changing salinity. Relatively parameters osmolarity, sodium, chloride, magnesium, cortisol, calcium, hematocrit, hemoglobin, number of red blood and white cells in water saltier than freshwater environment was higher (0.05〈 p, Duncan). Changes in hematological and blood plasma ionic parameters and vital organs Fingerlings indicates a willingness adaptability and the ability physiological adaptation fingerling was consistent with changes to environmental salinity brackish water .So, the factors measured with increasing salinity the uptrend that the range of variation for the osmolarite 449-281 mOsmol kg, for sodium, chloride, magnesium, cortisol, respectively, 211- 151, 165121, 3/3 7/0, 87. 53 mmol and the calcium 22-13 mg per dL. The measured values for hematocrit 32.2- 38.8%, hemoglobin 6.2 - 8.6 g per deciliter and the red and white blood cells, was respectively, 1.2-1.7×106 and the 15.6 -18.9×103.
    Keywords: Aquaculture ; Biology ; Iran ; Caspian Sea ; Adaptation ; Rainbow trout ; Gills ; Kidney ; Survey ; Oncorhynchus mykiss ; Fingerlings
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25835 | 18721 | 2018-10-13 10:49:48 | 25835 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was conducted for investigation on algae bloom monitoring and oscillations of biotic and non-biotic parameters in coastal waters of Bandar Abbas from October 2013 to Septembre 2014. In this project, planktonic distribution and environmental data were collected from five stations, every 10 days. Four order and 45 genus of phytoplankton (Bacilliarophycea 29 genous, Dinophycea 13 genus, Cyanophycea 2 genus, Raphidophycea 1 genus) were identified, as well as , 10 order of zooplanckton consisted of Copepoda, Mollusca, Chaetognatha, Sarcomastigophora, Urochordata, Annalida, Nematoda, Echinodermata, Ciliophora and Granuloretica. Results showed that Bacillariophycea were dominate among phytoplankton species in all season. So that, Leptocylindrus, Chaetoceros and Nitzschia from Bacillarriophycea, Protoperidinium and Gymnodinium belong to Dinophycea, and Oscillatoria belong to Cyanophycea were dominate genus in Bandar Abbas coastal waters. The maximum and minimum density and frequency for phytoplankton were recorded respectively from Shilat Jety and Airport Stations. Based on ANOVA analysis on phytoplankton density no significant were observed seasonally (p〉0.05), but significant differences were observed between stations (p〈0.05). Algal blooms were recorded for Protoperidinum, Chaetoceros and Noctiluca form Shilat Jety in the study period. Result showed that Crustacea have more density amongst zooplankton community, although Arthropoda were dominate in all season. The maximum frequencies were recorded for Nauplius (78%) and Copepoda (21%), also Among copepod, Calanoida order observed 68.5% frequency in the study period. The ANOVA analysis showed significant differences both in stations and seasons for zooplankton density (p〈0.05). The seasonal range of temperature were recorded between (19.43±2.58 - 31.98±2.48 ), salinity (27.15±9.48 - 38.13± 0.72ppt), dissolved Oxygen (2.8±1.81-6.87±1.25) mg/L, and pH (8.06±0.49- 8.69±0.23). Evaluation of biotic and abiotic factors indicate the probability of unhealthy and unstable Shilat jety and Gorzoozan stations coastal waters of Bandar Abbas City. One of these cases can be mentioned to high density of cyanophyceae in the estuary Gorzoozan compared other stations , high density of Phytoplankton and low diversity in the Shilat station. While the increase in Harpacticoida order of copepods As an indicator of pollution than on previous reports of other researchers In the this area, suggests pollution is pushed into the area.
    Keywords: Ecology ; Iran ; Persian Gulf ; Bandar Abbas ; Environmental parameters ; Algal bloom ; Phytoplankton ; Zooplankton ; Survey ; Planktonic communities
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25839 | 18721 | 2018-10-13 10:48:08 | 25839 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The aim of this project is to study water quality and the trophic status at different months and depths in the southeast Caspian Sea-Goharbaran based on environmental parameters and nutrients during 2013-2014 in order the feasibility of fish cage culture. Results showed that water temperature, transparency, pH, dissolved oxygen, BOD5, COD, total alkalinity and TSS were varied 9.00-29.00C, 0.50-12.00 m, 8.05-8.74, 5.76-12.85, 14-101, 21-195 and 0.00-0.12 mg/l at the different months and depths, respectively. In addition, the values of NH^4+, NH_3, NO^2-, NO^3- and PO^4-3 were 0.007-0.051, 0.001-0.010, 0.002-0.015, 0.043-0.477 and 0.014-0.077 mg/l, respectively. Chl-a concentration was recorded as 0.060-8.02 mg/l. Scaled and unscaled trophic indices were equal to 3.42-5.52 and 2.61-5.85, respectively. The proper temperature for cultivation of salmonids species in this area began from October to March. The range of the standard of pH and dissolved oxygen level for fish farming cage were 7.80-8.50 and 〉5 mg /l that in current study results of pH and dissolved oxygen which were consistent in the current study. Various forms of nitrogen (ammonium, nitrite and nitrate ) at the different layers were acceptable and less than the threshold limit of Australia and New Zealand. The results of the nutrients concentrations compared with data from the reference years (1996-1996 ) in the Caspian Sea (Goharbaran region) showed that this ecosystem passed from oligotrophic status and shifted to mezotrophic and eutrophic condition. As a conclusion, although nutrients concentrations were acceptable in terms of fish farming cages in this region, some parameters such as chemical oxygen demand and transparency are not suitable for aquaculture industry. Meanwhile, based on various trophic levels, this ecosystem (Gohrabaran region) has a mezotrophic status with a high risk of eutrophication.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Goharbaran ; Physico-chemical parameters ; Water ; Fish cage culture ; Temperature ; Transparency ; pH ; Dissolved oxygen
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25260 | 18721 | 2018-09-07 07:24:11 | 25260 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In order to study the Inheritance of Microsatellite Markers in the hybrid of Chalcalburnus chalcoides and Vimba vimba persa in the Southern part of the Caspian Sea, hybridization between the species was carried out during spring (2009) in Shahid Ansari reproduction and culture workstation. 30 samples from the produced larva and also small piece of dorsal and pectoral fin of their parents were collected and stored in pure ethanol (96%) and transferred to the biotechnology of the Caspian Ecology Academy. DNA was extracted from fin samples of the parents, whilst the whole F1 progeny was used to isolate the DNA by Phenol-chloroform method. Quantity and quality of extracted DNA was determined using 1% Agarose electrophorus. DNA was used for PCR ampilification of 10 microsatellite loci. The amplifications were run on 6% Polyacrylamid electrophorus. The present study showed that 7 out of 10 markers were polymorphic and variated between the parents. Goodness of fit to the Mendelian inheritance ratio was determined by X2 analysis. 4 primers were segregrated according to Mendelian expectations at a significance level of P 0.05. Based on UPGMA dendrogram (Nei, 1978) two different clusters were obtained in which Chalcalburnus chalcoides and hybrid were in the same cluster but Vimba vimba persa was in the other. It seems that Vimba vimba persa is a joint ancestor for these two. High heterozygosity presence in parents and their F1 shows the high potential of genetic variety in Chalcalburnus chalcoides and Vimba vimba persa which from the aquaculture management and conservation view is important and considering the high genetic variety in their hybrid, the hybrid has the sufficient potential for race improvement.
    Keywords: Biology ; Iran ; Caspian Sea ; Chalcalburnus chalcoides ; Vimba vimba persa ; Hybrid ; Microsatellite ; Hybridization ; Culture ; Samples ; Larvae ; DNA ; Inheritance ratio ; Heterozygosity
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25272 | 18721 | 2018-09-07 08:25:12 | 25272 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This experiment conducted to evaluate the effect of different dietary and bed treatments on earthworm (Eisenia fetida) for aquaculture. Concurrent with initial proliferation of earthworms, a pilot experiment also performed. In pilot experiment that conducted to become familiar with specialty and requirements of Eisenia fetida earthworms, lasted 4 months. The pilot treatments were including: 1) cow manure with covering its surface (control group), 2) cow manure (C.M.) without covering its surface, 3) C.M.+ 5% wind sand, 4) C.M.+30% saw dust, 5) urban compost, 6) C.M.+20% alfalfa meal, 7) C.M.+ 20% wheat bran and 8) C.M.+20% rice bran. After initial proliferation of earthworms and performing a pilot experiment, two trials designed. The first trial performed in outdoor environment, inside the plots dugged in the earh, with six treatments. The treatments were including: 1) cow manure(control group), 2) cow manure (C.M.) with vertical ventilation, 3) C.M.+ 30% saw dust (S.D.), 4) C.M.+30% S.D. with vertical ventilation, 5) urban compost+20% C.M. and 6) urban compost+40% C.M. The experiment lasted 6 months. In the end of trial in every plot, from 4 points, sampling for number of mature and immature earthworms, cocoons and the weight of earthworms were measured. The second trial performed in greenhouse by using wooden boxes. In this trial the treatments were including: 1) C.M. as control group, 2) C.M.+30% S.D., 3) C.M.+30% S.D.+ 10% alfalfa meal, 4) C.M.+30% S.D.+10% wheat bran, 5) C.M.+30% S.D.+10% rice bran, 6) C.M.+30% S.D.+10% soil, 7) C.M.+30% S.D.+5% fine sand, 8) C.M.+ 30% S.D.+1% lime powder and 9) C.M.+30% S.D.+200 ppm ammonium nitrate. Every treatment performed in 3 replicates. The earthworms reared in controlled condition for moisture and temperature, for about 4 months. The pH of treatments measured monthly by paper pH meter and by method of solution extraction. In the end of trial the number of mature and immature worms, cocoons and the weight of earthworms in every box were measured and the average weight of every earthworm calculated. The results of pilot experiment showed that covering surface the bed is necessary for maintaining moisture and darkness for earthworms. In wind sand treatment, the bed had sticky and compacted statues and appeared that the earthworms moved and activated difficultly. The saw dust treatment had a wet- spongy environment and the earthworms distributed well in it and their size was larger than control. Urban compost treatment unlike its suitable pH (equal 8), hardly accepted by the earthworms and they stayed in the corner of bed for more than one month. The wheat bran and rice bran treatments, both had sticky texture at the beginning but by spending time, their beds improved. The results of first trial showed the Eisenia fetida earthworms can survive and growth in outdoor environment even in cold season of winter. In this trial the highest total number of eartworms and cocoons and the total weight of earthworms was belong to control group, that had significant difference with the treatments C.M. + 30% S.D., urban compost + 20% C.M. and urban compost + 40% C.M. (p〈0.05). The performing of ventilation in experimental treatments had not significant effect on the number of mature earthworms, total number of earthworms and their total weight compare to control group. The urban compost was not suitable for rearing Eisenia fetida, although addition of 20 and 40 % of cow manure could improve it for usage by the earthworms. The results of second trial showed that, addition of alfalfa powder, wheat bran, rice bran and lime powder to cow manure had not significant effect on the total number and total weight of earthworms compare to control group. The usage of soil in treatment C.M. + 30% S.D. + 10% soil caused significant decrease in number of mature earthworms compare to control group (p〈0.05). The treatments including addition of saw dust and saw dust + fine sand to cow manure increased the number of mature earthworms, cocoons and total weight of earthworms compare to control group significantly (p〈0.05) and treatment of ammonium nitrate caused significant increase in total weight of earthworms (p〈0.05) .
    Keywords: Aquaculture ; Iran ; Diet ; Treatment ; Earthworm ; Eisenia fetida ; Aquaculture ; Dietary ; Ammonium ; Nitrate ; Temperature ; pH ; Mature ; Immature ; Growth
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25276 | 18721 | 2018-09-07 07:59:49 | 25276 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Following to introduce Vannamei shrimp (Litopenaeus vannamei) into Iran aquaculture industries by fisheries organization its postlarvae were successfully reared in ponds in Bushehr and Choebdeh in Khuzestan. Due to activity of Shrimp site and importance of input and output water quality, this study was done in 11 stations including: two stations in Bahmanshir river, two station in C4 and C5 irrigation canals, six staions in active farms along C4 cannel (Yonesi, Mosavei and Salman Zadeh), and C5 cannel (Mohamadi, Khairi and Ashraf Por) and one staion in output draing were selected. Sampling were carried out from May2009 befor beginning stocking to harvest time in October 2009. Physico- chemical parameters and plankton sampled biweekly and benthic animals and heavy metals were sampled monthly. Some parameters such as pH and temperature by using portable multy parameters and others. Analyzed with standard methods. Heavy metals were measured by plarography method (797 Vamtro). Plankton and benthos samples were studied only in Bahmanshir station. According to results the maximum values for DO (11.1 ppm) PO4 (1.86 ppm) NO3 (8.4 ppm) and TSS (4992 ppm), pH (8.4) NO2 (0.18 ppm) BOD5 (9.06 ppm) were measured. In comparison to aquaculture water quality standards except for TSS in river stations others are located in allowable range. We didn’t observed any main changes in water quality from river to output drainage. The results of biotic parameters in river stations showed that diatoms were the most group of phytoplanktons. Zooplankton groups were Copepods, Rotifera and Protozoa. Tintinids and Nematoda larvae were the most abundant zooplankton. Benthic animals were included nine crustacean species and two annelid species. Isopods with 52% were the most and ant group.
    Keywords: Aquaculture ; Ecology ; Iran ; Khuzestan Province ; Abadan ; Choebdeh ; Bahmanshir River ; Vannamei shrimp ; Litopenaeus vannamei ; Water quality ; Abadan aquaculture site ; Rearing ; Shrimp ; Ponds ; Sampling ; Plankton ; Heavy metals ; pH ; Temperature ; Benthos ; Phytoplankton ; Zooplankton ; Copepods ; Nematoda larvae
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    Publication Date: 2021-07-16
    Description: Rutilus frisii kutum is one of the important fish of Caspian Sea which has significant economical role in the region .The objective of this project is a comparative study on traditional culture of fishes versus slurry. This survey were conducted on nine pound of 1.7 hec triplicate with their treatment with stocking densities of 1.7 million fish larva at Dr yousefpoor center (Affiliated of Shahid Beheshti culture and propagation) at Siakal village , 32 km far from Rasht city in North of Iran, the first treatment considered as control which practice traditionally (using concentrated food plus grinded kilka fish) .In the second treatment for 13 days the pound supplied only by slurry then followed by concentrated fish food only .The tired treatment started with slurry for 13 days and followed by slurry plus concentrated food. The physical and chemical parameters of water, plankton, biometry of fish, growth indices such as daily weight growth (DWG), daily length growth (DLG) as well as specific ratio (SGR) were monitored weekly. The result indicate that net fish production was 1.7 to 2 times higher in slurry than traditional treatment also the survival rate were 1.7 times higher in slurry treatment . The treatment of slurry follow by addition of concentrated food plus slurry showed higher yield camper to others. Slurry with several active substances is more effective and can promote the growth of zooplanktons which is the food of larval stage of rutilus frisi kutum. In second phase of this project the effect of slurry was surveyed on Chinese carp. This experiment was conducted in triplicate with two treatment (one treatment is control which use cow manure plus chemical fertilizer and second treatment was conducted with slurry) and stocked with 2375 N/hec (Silver carp 60%, Big head 15%, common carp 17% and grass carp 8%) at June. In this survey common carp and grass carp were feed with concentrate food and fresh grasses respectively. Chemical factors were measured once for each two week and density of plankton was determined when it was necessarily. Nutritional content of phosphorous, nitrogen, calcium, potassium and magnesium after anaerobic fermentation as well as protein and lipid content with 1.4and 1.9 times respectively were higher in slurry treatment than control. The results showed that survival rate and yield were higher in slurry than control. The increasing percent of yield were 13.5, 2.6, 18.4 and 85.3 in Silver carp, Big head, common carp and grass carp respectively. The survival rate of grass carp was twice higher in slurry treatment than control. Zooplankton abundant in slurry pounds was more but blue- green algae density was less than control. Protein and lipid analysis reviled no significant difference between control and slurry. In general the result indicate that the slurry with higher nutritional content is more effective on the survival and growth rate of fishes and also is more efficient in proliferation of plankton in particular zooplankton.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Slurry ; Rutilus frisii kutum ; Cow fertilize ; Larviculture ; Carp ; Culture ; Plankton ; SGR ; Zooplanktons ; Silver Carp ; Big head carp ; Common carp ; Grass carp ; Phosphorous ; Nitrogen ; Survival rate ; Protein ; Lipid
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25301 | 18721 | 2018-09-08 16:29:41 | 25301 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was performed as a research program in Agricultural Research center of Kerman since 2000. During one year identification of fish in qanat basins of Markazi desert and Sirjan desert was carried out. In this study Electroshoker and salik net with o.5 and 1 cm network and padel net with 1cm were used to catch the fishes from 73 series of qanat. Parameters estimated in qanats (pH, Weather Temperature, Oxygen , Ec,...) Specified in sum 767 from fishes which were in 2 order 3 family and 3 species as: 1-Order: Cypriniformes. Family: Cyprinidaea Species: Capoeta damascina (Valenciennes, 1849) family: Balitoridaeb species: Schistura sargadensis (Nikolskii, 1900) 2-Order: Cyprinodontiforms Family: Poecilidaea Species: Gambusia affinis (Baird and Girard, 1853)
    Keywords: Biology ; Iran ; Kerman ; Sirjan ; Qanats ; Fish ; Capoeta damascina ; Gambusia affinis ; Schistura sargadensis ; pH ; Weather Temperature ; Oxygen ; Species ; Cypriniformes ; Cyprinidaea ; Balitoridaeb ; Cyprinodontiforms ; Poecilidaea ; Identification
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25304 | 18721 | 2018-09-08 16:49:09 | 25304 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The Common carp is the most important culture fish in the word, that can be consider as domestic fish. The Common carp of Caspian Sea is one of the economic fish, that have some morphenological differences, resistance in salt water and better quality in meet with domestic once and for using this fish in culture system, it is necessary to establish genetically parameters especially the heritability for selection purpose. The present study have been started from 2007 and lasted 3 years. The breeders were collected from the Gomishan station in south-east of Caspian Sea by fisherman and stocked in S.Rajaei fish restocking center and propagate based on the routine propagation technique in this farm. For heritability estimation a factorial crosses by 7×7 fish were done. Therefore, the fingerlings of 49 crosses were achieved, that kept first separately fiberglass tank and then in pond separately. Weight and length of fish were choice for heritability estimation. The average weight of juvenile common carp in 3 sampling stage of 90, 180 and 360 days, were 5.9, 35.7 and 84.7 gram and length of 9.6, 14.7 and 19.2 cm respectively. The heritability estimation for weight was 0.26 and length 0.25. Selection response based on selection difference by collection the more average breeders among their progeny were 36.4 g that means by selection every generation, there is about 4 percent improving in fish weight.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Gomishan ; Common carp ; Cyprinus carpio ; Heritability ; Selection Response ; Culture ; Weight ; Length ; Fish ; Sampling ; Generation
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25326 | 18721 | 2018-09-12 10:09:55 | 25326 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Recently, the ecology of the Caspian Sea coastal has undergone with increasing development of human activities and industrial wastewater discharge. Therefore, considering the current situation, evaluate of pollutants issue in the Caspian Sea is very important. Results of this study showed that levels of heavy metals (Cd, Cr, Cu, Fe, Pb, and Zn) of water had lower than standard and also acceptable for aquatic life. Significant accumulation of metals such as Co, Cu and Cr in the sediment was observed at transect Sefidrood and Anzali in comparison with six other transects (Turkmen, Amirabad, Babolsar, Noshar, Tonekabon and Astara) and for Ni at transects Sefidrood and Astara compared with six other transects (Turkmen, Amirabad, Babolsar, Noshahr, Tonekabon and Anzali) (P〈0.05). The amount of Zn in fish muscle Liza saliens was maximum and then metals such as Fe, Cu, Pb, Cd, Ni, Hg and Co were recorded (P〈0.05). In the north of Iran, two kind of bony fish, such as R..frisii kutum and Liza.s have maximum catchments as compared with other bony fish in the Caspian Sea. Based on calculation of risk of Target Hazard Quotient (THQ), we obtained THQ was less than one for all metals in young and adults human. Thus, consumption of R..frisii kutum and Liza.s per capita with 6 kg will not be at the risk for young and adults. Among of oil derivatives, polyaromatic hydrocarbon (PAHs) is hazardous compounds in the environment. Environmental Protection Agency (EPA) was announced 16 compositions of PAHs as an index. The results of this project showed that concentration of two and three rings of PAHs was lower than 4, 5 and 6 rings of water, sediment and fish at most stations and seasons. Among of 16 PAHs, only Benz(α)anthracene, chrysene, Benzo(α)pyrene and Dibenz (α, h)anthracene compounds were contained with ecological risks (HQ) more than one in the water and sediment. In addition, three compounds such as Chrysene, Benz(α)anthracene and Benzo(α)pyrene were observed in the edible fish tissues (Liza.s and R..frisii kutum) which have high ecological risks in the water and sediment. During four seasons (spring, summer, fall and winter), maximum concentration of organochlorine residue in the surface water (5m depth) were belong to β-Endosulfan (Turkmen), Dieldrin (Babolsar, Tonekabon) and endosulfan sulfate (Astara) and at 10m depth were γ-BHC, Dieldrin compounds (Tonekabon), DDT (Turkmen) and Aldrin (Babolsar). This values at 50m depth were belong to γ-BHC (Anzali), δ-BHC (Tonekabon), Dieldrin (Amirabad), and heptachlor (Amirabad). Maximum percentage of organochlorine pesticides residue in the sediments was belonging to only Aldrin compound at stations Turkmen, Babolsar and Tonekabon. In addition, maximum concentration organochlorine pesticides residue in fish tissue (Liza.s and R..frisii kutum) was DDT and Endrin aldehyde compounds. Mean surfactant concentration (LAS) of surface water (5, 10 and 50 m depths) at eight transects was 0.017±0.049 mg/l. The maximum concentration of LAS were 0.084 mg/l and 0.082 mg/l in spring and summer (Anzali transect), respectively. This value was 0.035 mg/l and 0.060 mg/l in autumn and winter (Sefidrood transect), respectively. As a conclusion, the concentration of surfactant in the Caspian Sea basin is not critical as compared with standard level.
    Keywords: Pollution ; Iran ; Caspian Sea ; Anzali ; Babolsar ; Astara ; Noshar ; Sefidrood ; Survey ; Pollutants ; Heavy metals ; Hydrocarbons ; Aquatic ; Bony fish ; R.frisii kutum ; Liza ; PAHs
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25329 | 18721 | 2018-09-13 12:35:03 | 25329 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This project were investigated seasonally from march 2008 up to one year, in southern Caspian Sea iranian coast /Sampling was carried out at 8 stations of between 5 and 100 m depth and taken in triple with a 0/1m² van Veen grab/Total twenty four species and one family and one order were identified, presenting low dominance and frequency/ polychaeties 56%,oligochaetes 39% bivalves 〈1% amphipodes1% Cumacea and diptera and cirrepedia %1 of whole macrobenthos compositions/ Polychaetes and oligochaeta dominated, representing 95% of the whole fauna/Average of species were 11/8 species per grab/max 21 species / grab were found in Tonkabon stations at 100 meter depths and minmum 4 species/grab in 20 m in Bandar anzali /Maxmiun density were 4783 n/m^2 in spring and minmum 3177 n/m^2 in winter/Highest density were recorded in Amirabad stations and lowest in nooshar/ Maximum density showed in east, mimun in middle area of sea The biomass of benthos adventages in west/Average biomass of whole area were 6/932 gr/m^2/Mean total organic matter were 3/11±1/1 and silt- clay 62/92±6/12precent and Mean dissolve oxygen and salinity were 8/3±0/15 mg/l and salinity 12/2 ±0/4 ppm and water tempreture were 14/9±0/18ْC/ Mean grain size, fine sand, very fine sand, silt and clay contents were among the main factors related to the patterns of macrofaunal distribution, density and diversity and dominance of trophic groups.
    Keywords: Ecology ; Iran ; Caspian Sea ; Tonkabon ; Bandar Anzali ; Macrobenthos ; Disturbiotion ; Diversity ; Productions ; Survey ; Diversity ; Abundance ; Biomass ; Macrobenthic Fauna ; Sampling ; Species ; Polychaeties ; Oligochaetes ; Bivalves ; Dissolved oxygen ; Salinity ; Temperature
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25330 | 18721 | 2018-09-13 12:40:49 | 25330 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Study of survey health management and diseases in hatcheries and fish farms can help us to knowledge and application control methods such as: prevention, treatment and increase high levels of production in hatchery and farms, finally. This survey carried out from 2005 to 2008 for 4 years in sturgeon hatcheries and farms of Golestan province. Sturgeon fishes include Huso Huso, Ship sturgeon, Acipenser persicus collected and for virology, bacteriology, fungius and hematology examined. Also, physicochemical parameters measured and recorded in different stages of culture. Results of this study showed that all of samples in virology was negative and did not observe any doubetful causes. In bacteriology CFU was variation from 3/9 ×105 to 6/9×10. The most parasites that detected in this survey was Cocolanus espherolanus, Sceria binopsulus semiarmatus and Amphilina fuliacea that separates from Acipenser Percicus, especially. The results about hematology parameters some important hematological indices of ship sturgeon include: The total RBC for female and mail specimens measured as 5.3±1.5 ×10^5, 4.8±0.5×10^5 per mm^3 respectively. The amount of haematocrit and hemoglobin for female and mail determined: 34.3±2.8, 35±1.4 percent and 10.3±0.9, 8.9±0.8 gr/dl .The MCV: 216.3± 96.2, 736.5± 102.5, MCH: 720.2±309.5, 186±0.7 and MCHC: 30±0.8, 25.5±3.4 percent respectively.The total WBC were (female, male): 21320±1054, 20580±777 per mm^3 and neutrophil: 16.4±2.5, 17±1.4 percent and lymphocyte: 74.4±2.4, 73.5± 0.7 percent and eosinophil: 6±1.4, 6.4±0.5 percent, monocyte: 2.8±0.8, 3.5±0.7 percent. There was not any significant differences (p〉0.05) between mentioned parameters in male and female (students t-test). Also evaluation of hematological parameters in bluga ( Huso huso) include: total RBC were (male , female) 5±0.3 ×105 , 4.9±0.6 ×105 per mm^3 ,respectively and hematocrit: 33.2±6.7 , 35.4±3.4 percent and hemoglobin: 11.2±1.5 , 12.2±1gr/dl and MCV: 669.9±172.2, 723.9±982.4 and MCH: 226.2±42.5, 249.5±35.4 and MCHC: 34.1±2.4, 34.6±3.6 percent respectively. The totals WBC were (male, female): 24800±707.1, 23042±1375.4 per mm^3 and neutrophil: 18.5±0.7, 21.4±1.1 percent and lymphocyte: 73.5±1.4, 68.4±1.1 percent and eosinophil: 5±2.8, 7±1.2 percent and monocyte: 3.5±3.5, 3.2±0.8 percent. According to statistically study the count of lymphocyte had significant difference between male and female fish and this count in male was higher than female. (p≥0.05).
    Keywords: Aquaculture ; Iran ; Golestan Province ; Mazandaran Province ; Guilan Province ; Fish ; Sturgeon ; Huso huso ; Ship ; Acipenser percicus ; Bacteriology ; Parasitology ; Health management ; Diseases ; Survey ; Aquaculture ; Hatcheries ; Samples ; Sceria binopsulus ; Amphilina fuliacea ; Female ; Male
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25353 | 18721 | 2018-09-14 07:27:04 | 25353 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Gillan , Mazandaran and Gorgan provinces have temperate climate, thus they have more potential for aquatic animal culture. There are thousands hectare of lands for Aquaculture and fisheries in adjacent to Caspian Sea in this provinces. These areas (North alborz) have provided a favorable back ground for aquatic animal breeding and stock enhancement of species in the sea. As a result, this investigation executed during two stages (phases). At first stage, the goal of this project included the feasibility study and demonstrating the existing situation of fish stock enhancement activities in North Alborz area and second phase is to survey on distribution, and production activities of stock enhancing hatcheries as well as their constructions, and production time table. Survey was started from 2006 to 2008 by using qestionaries in 7 hatcheries from tree provinces. Results reviled that there are more than 10 species was subjected to restocking .Total releasing was about 300 millions of fry and finger lings in to the Caspian sea.
    Keywords: Management ; Iran ; Caspian Sea ; Guilan Province ; Mazandaran Province ; Gorgan ; North Alborz ; Stock Enhancement ; Broodstock ; Production ; Yield ; Artificial propagation ; Bony fishes ; Sturgeon fishes ; Restocking ; Aquatic ; Culture ; Aquaculure ; Fisheries ; Breeding ; Survey ; Hatcheries ; Fry ; Fingerlings
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25363 | 18721 | 2018-09-14 07:46:07 | 25363 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This project was carried out in order to determine the hatching conditions and laboratory culture methods of Fairy Shrimps (Phallocryptus spinosa). Physico- chemical factors and phytoplankton of natural habitat of this organism was investigated in Khasellou region around Azarshahr – East Azarbaijan province. The results showed that occurance and growth of fairy shrimps in their habitates were beginning from early Aprill and their survival was decreased with increasing temperature, precipitation and increasing the salinity of their culture medium. Then they disappeared. Their habitat phytoplankton contained 3 phylla and 10 genera, from which green algae enjoyed the greatest density in May. The pool salinity was around 20-17 g/l, when metanauplii was observed in early spring. In the pool containing and without fairy shrimps the oxygen concentration was high (9 mg/l) and low (2.9 mg/l), respectively. In 9 prawn ornamental fish genera, feeding with fairy shrimp showed a significant higher fecundity and hatching percentage compared to manual diets (cow liver, spinach). In all groups fed with fairy shrimp the duration of spawning time were reduced from 15 to 8-10 days and the color was enhanced. The means of cyst number per captured female also, cyst, decapsulated cyst and Naupilli diameters were mesured 142.9±19.0 cysts, 273.2±4.9µ, 242.4±3.8µ and 542.6±27.0 µ, respectively. The hatching rate was increased by reducing temperature and salinity of their culture mediums from 25 to 15 ◦C , and 28 to 18 g/l, respectively. The highest hatching was observed on 5th day at 15 ◦C and 18 g/l (5, 33 %). In addition, low but multiple hatching was observed. Therefore, water temperature and salinity affected hatching rate in this species. The hatching success of Phallocryptus spinosa cysts was better when collecting cysts together with dry sediments of their habitat without separation sensitive cysts. In addition to cold keeping and freezing, the hydration – dehydration method caused more diapause deactivation for hatching induction. The laboratory results revealed that the survival of fairy shrimps were highest at 15 ◦C (42%) and lowest at 25◦C (26%), respectively. Also, their life was inhibited in 30◦C. In different salinities, the mean survival rate were 26% (Salinity= 15 g/l) and 20% (Salinity= 25 g/l), respectively. The mean survival percentage of Phallocryptus spinosa cultured in natural habitat water (64.3%) was higher than aerated tap water (23, 3 %). Also, feeding with Nanochlorepsis caused higher survival rates. On the other hand, Beakers yeast caused higher mortality rates. Therefore, regarding to relatively high sensitivity of these species to physico– chemical and nutritional conditions of their culture medium, it is better to culture these species with expansion of their natural habitat in order to preserve their biodiversity, culture and increase stocking density with regard to environmental considerations .
    Keywords: Aquaculture ; Iran ; East Azarbaijan province ; Fairy Shrimps ; Phallocryptus spinosa ; Hatching ; Culture ; Biotechnique ; Species ; Anostraca ; Phytoplankton ; Growth ; Temperature ; Salinity ; Phylla ; Feeding ; Survival rate ; Mortality ; Biodiversity
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25385 | 18721 | 2018-09-18 05:58:09 | 25385 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study ichthyoplankton, phytoplankton, zooplankton, benthic and hydrochemistry parameters were studied. 9 out of 11 sampling stations were in the Gorgan Bay area and 2 of them were in the mouth of the Bay. Ichthyoplankton survey were carried out in 6 stations and the other surveys such as phytoplankton, etc were done in 11 stations in 2009. Gorgan Bay map and position of sampling sites are given at the end of material and methods section. Biomass and frequency of phytoplankton at different stations, months and seasons were calculated that are presented in Tables 1 to 3. Hydro-chemical parameters such as water temperature, air temperature, visibility, DO, BOD5, pH, Alkalinity (bicarbonate, carbonate and total), Hardness (calcium, magnesium), CL,EC, Salinity, N (nitrate, nitrite, NH_3), Phosphate were measured and results are shown Table 11. Biomass (mgr/m^3) and numbers (ind./m^3) of 47 genus of different phylum of phytoplanktons such as Cyanophyta, Chlorophyta, Pyrophyta, Chrysophyta and Euglenaphyta as well as different phylum of zooplanktons consist of Protozoa, Arthropoda, Rotifera and Cyliophora were estimated. Frequency of phytoplanktons (ind/m^3) was 131.2, 1.6, 65.3, 18.9 and 23.8 million respectively. Furthermore, their biomass was 308.4, 1.9, 1358.9, 295.1 and 124.7 mg/m^3, respectively. The maximum and minimum numbers were observed in Leptolingbaya belonged to Cyanophyta and Diatoms of Chrysophyta 84.3 and 0.025 million (ind/ m^3) respectively. The maximum and minimum biomass was observed in Gyrosigma (Chrysophyta) and Tetradron (Chloropyta), 12317.3 and 0.014 mg/m3 respectively. The average biomass of zooplankton was measured 531.74, 30.77, 225.07, and 96.26 mg/m^3 respectively and the total was attained 883.83 mg/m3 Table 5. The maximum and minimum biomass of all zooplanktons was estimated in November and July, 3446.23 and 6 mg/m^3 respectively. Furthermore, the maximum and minimum numbers of zooplanktons were found in September and July which were 48.4 and 0.002 millions respectively and the average number was 8.4 ind/m^3 Table 4. Also numbers and biomass (gr/m^2) of 11 families of benthos which inhabit Gorgan Bay such as Annelida, Mollusca and Arthropoda were investigated in separated stations and distinctive months. The maximum and minimum biomass of benthos was observed in January (42.91 gr/m^2) and March (0.6428 gr/m^2). The biomass was considerable in April, February, August and December (Tables: 4, 5 and 6).
    Keywords: Ecology ; Iran ; Caspian Sea ; Gorgan Bay ; Ecological study ; Fisheries ; Ichthyoplanktons ; Phytoplankton ; Zooplankton ; Benthic ; Sampling ; Biomass ; Temperature ; Visibility ; pH ; Alkalinity ; Salinity ; Phosphate ; Cyanophyta ; Annelida ; Mollusca
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25394 | 18721 | 2018-09-18 05:38:30 | 25394 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: For the improve handling of carp cultured fishes in Kermanshah province we chose 4 treatment out of that one treatment was the best, this included fish+powdered ice with C.S.W tank transported with a roof covered car in all treatment fish are tested physically and chemically and microbiologically, also temperature of fish body and environment was taken periodically andattempt for biometry and weight ranges of fishes also did in each study.
    Keywords: Aquaculture ; Iran ; Kermanshah province ; Warm water fish ; Culture ; Carp ; Weight ; Fish
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25405 | 18721 | 2018-09-20 12:38:52 | 25405 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The project of the Study on fishing and resource management of fisheries within Southern Caspian Sea was conducted on the base of an agreement made between fisheries organization (Shilat) and the Iranian fishery research organization (IFRO) signed in 2010. The study has several phases. The final report of the fishery on fishing and resource management of fisheries within Southern Caspian Sea (Gilan, Mazandaran & Golestan provinces) is consisted of several sections such as: 1- The Survey status of biology and catch of Kilka fishes , Bony fishes and Estrogen fishes and also the status of aquatic resource conservation in the Southern part of Caspian Sea 2- Fishing analysis in the Southern part of Caspian Sea 3- Fisheries development Strategies, policies and plans in the Southern part of Caspian Sea In this survey and library-based study, internal and external environmental factors of shilat activities are considered. Using SWOT analysis method, current situation of fisheries is investigated. In this regard, all fisheries activities were divided into four groups, then, the strengths, weaknesses, opportunities and treats of internal and external environment will be analyzed. A considerable number of tables and matrixes were prepared and policies and strategies as seen of final report were carried out.
    Keywords: Fisheries ; Iran ; Caspian Sea ; Gilan province ; Mazandaran Province ; Golestan Province ; Resource management ; IFRO ; Shilat ; Fishing ; Kilka ; Fisheries ; Survey ; Biology ; Bony fishes ; Estrogen ; Environmental factors
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25412 | 18721 | 2018-09-23 15:56:29 | 25412 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Using SWOT analysis with traditional matrix and quantitative matrix methods, the strategies to benefit from strengths and opportunities to vanishing weaknesses and decrease of threats for strategic management of aquaculture in Iranian Northern provinces was surveyed. Utilizing researches held in fisheries research centers and existence of aquaculture experts in vicinity, activation of veterinary organization in production and post production, and entering new brood stocks with desirable growth are the main strategies for developing rainbow trout and Chinese carps culture in Iranian Northern provinces. In sturgeon fishes culture, independence of farms from fingerlings produced by governmental complex and diversification the cultured fishes using new species and hybrids can develop this part of aquaculture. Avoiding illegal fishing in sea and rivers, preserving the population of Caspian Sea fishes, entering new fish species which their resources are decreased extremely to breeding and fingerling production cycle, and using pike perch and bream while they migrate to Anzali Lagoon from Caspian Sea, instead of freshwater brood stocks will cause the output of sea enhancement and sea ranching activities to increase, and fisheries return coefficient of these fishes will rise. Using quantitative matrix and scoring method, it was defined that aquaculture in Iranian Northern provinces are extremely approved and acceptable and the score of strengths is more than weaknesses and opportunities more than threats. By using strategic management, these activities can be more developed.
    Keywords: Management ; Iran ; Caspian Sea ; Anzali Lagoon ; SWOT ; Strategic management ; Aquaculture ; Sea enhancement ; Sea ranching ; Traditional matrix ; Quantitative matrix ; Fisheries ; Survey ; Culture ; Species ; Illegal fishing
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25428 | 18721 | 2018-09-23 16:44:06 | 25428 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In order to "An investigation designing a suitable plan for sholam research warm water fish ponds and culture and hatchery", survying some of refrences and scientific papers were done. As a result the infrastructures of that plan were determined, and the diagram for relation between structures was designed. Finally according to the diagram,three plans were distincted, and some of them with desire advantage was selected.
    Keywords: Aquaculture ; Iran ; Plan ; Fisheries research center ; Relation diagram ; Culture ; Hatchery ; Fish pond ; Surveying
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25403 | 18721 | 2018-09-20 12:22:36 | 25403 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Sediments samples were collected using Veen Grab Sampler (0.1 square meter) at 8 transects namely Astara, Anzali, Sefidroud, Tonekabon, Noshahr, Babolsar, Amirabad, Torkman in the southern of Caspian Sea. Five stations were selected at 5, 10, 20, 50 and 100 meter depths in each transect. Sediments were sampled triplicate at each station. Samples also were collected during four seasons (spring (May), summer (July), fall (November) and winter (January)) in 2009. Results of this study showed that species composition of Macrobenthos consisted of 32 species which belonged to 7 families of Polychaeta, Crustacea and Bivalvia at studied area. In addition, Oligochaeta identified in “Class”, Chironomidae considered in Insecta categories “Family” and Streblospio spp. (Polychaeta) was recognized in “Genus”. Gammaridae and Pseudocumidae of Crustacea with 12 and 10 species had the highest species diversity compared to other groups, respectively. Polychaeta was consisted 75.5 percent of total abundance of macrobenthos which the major abundance (equal 62.4% of total abundance) were belong to Streblospio spp. from Spionidae family, while its biomass was equals 5.11% of total macrobenthos. In contrast, Cerastoderma lamarcki species from Bivalvia Class with only 1.7% of total abundance of macrobenthos allocated 69 percent of total biomass. In the southern of Caspian Sea, average total abundance was significantly less at 4 western transects (Astara, Anzali, Sefidroud, Tonekabon) compared to 4 eastern transects (Noshahr, Babolsar, Amirabad, Torkman) (p〈0.05). The highest average abundance of macrobenthos (10655±1246SE ind/m^2) was observed at transect of Torkman, and lowest value (4032 ± 686SE ind/m^2) was recorded at transect of Sefidroud (p〈 0.05). Generally, minimum species diversity were obtained at 20 m depth in all transects and the maximum value was observed at 5 m depth in most of transects (p〈0.05). In contrary, maximum average abundance of Macrobenthoses was at 20 m depth in transects of Anzali, Sefidroud, Tonekabon, Nowshahr and Amirabad compared to other depths. Macrobenthoses abundance average in 5 m depth (except Astara and Torkman) was less than other depths in 6 transect (p 〈 0.05). Total average abundance and biomass of macrobenthos was 5976±583SE ind/m^2 and 43.675 ± 11.402SE gr/ m^2, respectively. Maximum and minimum of abundance of macrobenthos were observed in summer (7714±778 ind/m^2) and winter (4071 ± 340 ind/m^2), respectively. Maximum and minimum of biomass of macrobenthos were obtained in fall (50.271±13.258SE gr/ m^2) and in summer (35.123 ± 8.903SE gr/ m^2), respectively (p〈 0.05). Percent of total organic matter (TOM) were low in 5 and 10 m depths and increased toward offshore depths. TOM percent was 2.06±0.11SE at 10 m depth and increased to 4.62 ±0.17SE in 100 m depth. Percent of silt and clay (grains size less than 63 micron) had positive significantly correlation with percent of TOM (p〈0.01).While they had negative significantly correlation with percent of sand (grains size between 63 and 1000 micron) (p〈0.01). Percent of silt and clay like organic matter, had ascending trend toward to depth increased and varied from 44.4 ± 4.06SE percent in 5 m depth to 96.5 ± 0.59SE percent in 100 m depth. In contrast, percent of sand decreased toward depth and varied from 54.5 ± 4.13SE percent in 5 m depth to 2.8 ± 0.53SE percent in 100 m depth. Result of current study showed that total abundance of macrobenthoses had positive significantly correlation with TOM percent (p〈0.01) and silt/clay percent (p〈0.05). Abundance of Oligochaeta had positive significantly correlation (p〈 0.01) with TOM and silt/clay percent. Two groups of Polychaeta, Gammaridae and Cerastoderma lamarcki had negative significantly correlation with TOM and silt/clay percent (p〈 0.01), and every four aforementioned groups had positive significantly correlation with sand percent (p〈 0.01). Overall, different correlation between abundance of various macrobenthos groups and TOM percent and type of grain size of sediment could be related to fluctuation of abundance of various macrobenthos groups at difference transects and depths. On the other hand, in study area were occurred simultaneously some phenomena such as increased abundance of Oligochaeta and Polychaeta, dominance of Streblospio Genuse (Polychaeta group), and decreases abundance of Bivalvia and appearance of Menemiopsis leidyi which need to study more and monitoring of this area.
    Keywords: Ecology ; Iran ; Caspian Sea ; Astara ; Anzali ; Tonekabon ; Noshahr ; Babolsar ; Sefidroud River ; Macrobenthos ; Diversity ; Distribution ; Abundance ; Biomass ; Survey ; Fauna ; Sediments ; Samples ; Streblospio spp. ; Cerastoderma lamarcki ; Menemiopsis leidyi
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25413 | 18721 | 2018-09-23 16:29:08 | 25413 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This survey was carried out by R/V Gilan with a conical plankton net of 100 micron mesh by vertical hauls at 8 transect in the southern Caspian sea. Transects were located at Astara, Anzali, Sefid roud, Tonekabon, Noshahr, Babolsar, Amirabad and Torkman in depths of 5, 10,20,50and 100m. Samples were taken in 4 seasons (in each season 64 samples) in 2009. In this study 23 species formed the zooplankton community including 4 species of Copepoda, 8 species of Rotatoria, 2 species of Protozoa and 8 species of Cladocera. The meroplankton blonged to 2 species of Balanus sp and Lamellibranchiata larvae. The annual results revealed that the maximum abundance of Copepoda were 3612±4839 ind/m^3 and 35/75±42/81 mg/m^3 in summer and decreased gradually since autumn and reached to minimum value in winter. The maximum abundance of Cladocera was 300± 383 ind/m^3 in spring and reached to less than 4 ind/m^3 in summer and autumn. The Rotatoria had high population (3775± 8632 ind/m^3) in winter which consisted the zooplankton population with Copepoda. The Protozoa had no efficient role in zooplankton population of Caspian sea during the year. The zooplankton population was affected by Meroplankton and Lamellibranchiata larvae in spring and winter. The Copepoda contributed in zooplankton population only in summer and autumn. The maximum zooplankton abundance and biomass were in 5m depth which included 10209±8352 ind/m^3 and 43.56±26.08mg/m^3 in spring, 8545±7127 ind/m^3 and 73.59±56.65 mg/m3 in summer (10 m depth), 8427±6711 ind/m^3 and 43.15± 29.59 mg/m3 in autumn and 24426±26975 ind/m^3 and 334.25±392.21 mg/. m3 in winter which decresed from surface to depth. The maximum abundance and biomass of zooplankton were 5119±6018 ind/m^3 in east and 28.59±24.79 mg/m^3 in central area in spring, 6628±7117 ind/m^3 in west area and 50.73±59.52 mg/m^3 in central area in summer, 5248±5489 ind/m^3 and 31/16 ±31/22 mg/ m^3 in west area in autumn and 11588±16191 ind/m^3 and 131.02±193.61 mg/ m^3 in west area in winter. The annual statistical analysis reveals that there is significant difference between Copepoda and Cirripedia population among seasons, sampling stations, depth and sampling layer (Kruskalwallis test, p〈0.05) and the total zooplankton populatin had no significant difference only between seasons (Kruskal-wallis test, p〉0.05). The Cladocera, Lamellibranchiata larvae and the Rotatoria had significant difference between seasons only (Kruskal-wallis test, p〈0.01).
    Keywords: Ecology ; Iran ; Caspian Sea ; Astara ; Anzali ; Tonekabon ; Noshahr ; Babolsar ; Zooplankton ; Copepoda ; Meroplankton ; Survey ; Plankton ; Samples ; Species ; Cladocera ; Meroplankton ; Balanus sp. ; Lamellibranchiata ; Larvae
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    Publication Date: 2021-07-16
    Description: In this study, first, the influence of three precooking methods (steaming, oven-baking and microwave-cooking) on the contents of mineral elements-copper, zinc, iron, calcium and sodium-of silver carp (Hypophthalmichthys molitrix) was evaluated. Determination results of evaluated elements in raw fish and cooked samples were in range 3.05 to 4.19 for copper, 71.45 to 82.85 for zinc, 32.18 to 40.70 for iron, 425.6 to 529.46 and 315.5 to 534.76 for calcium. Results showed different precooking treatments had no significant effects on the amounts of mineral elements such as copper, zinc, calcium and sodium. The Iron content in the samples subjected to microwave cooking increased. With doing multivariate analysis, on comparing the raw and precooked fillets, steam cooking found to be the best precooking method on retain mineral elements. After choosing the best precooking method, for evaluating the influence of different filling media including sunflower oil, soybean oil, olive oil and brine, on the quality of canned silver carp, amounts of mineral elements(copper, zinc, iron, calcium and sodium) and microbial indices(total counts, thermopile count and clostridium) and sensory indices (color, smell, taste and texture) was surveyed. Assessments of microbial indices showed no microbial activity in canned products. After canning amounts of sodium increased in all the treatments. Except for iron, using soybean oil had no significant effect on the contents of other elements. The highest levels of iron and copper were observed in olive oil canned samples sterilized at 130°C. Sensory evaluating showed, kind of filling media had no significant effects on sensory indices such as taste, smell and color of canned samples. The texture of soybean oil canned samples and the quality defects of olive oil and brine canned samples had better condition than other treatments. In the last step, canned silver carp were proceed under three different temperatures (115°C, 120°C and 130°C) with equal lethality value (Fo=7min), then sensory indices and amount of mineral elements were compared. Results showed, the contents of iron, copper, sodium and calcium were changed in soybean oil canned sample. The amounts of copper and sodium in sunflower oil canned sample subjected to different heating regimes showed significant variation. The highest amount of copper was observed in the canned samples subjected to 130°C heating regime. Results showed contents of iron and copper of olive oil canned sample subjected to 120°C and 130°C heating regimes were higher than sample subjected to 115°C heating regime, while the zinc and calcium contents had no variation. In brine canned samples the highest amounts of copper and iron was obtained after sterilization in 120°C. Results of sensory evaluation showed different heating regimes had no significant effects on the indices of taste, smell, and color of products. Doing 130°C heating regime in brine canned samples led to obtain the better tenacity of texture. While this heating regime caused to increase the quality defects of soybean oil canned samples as a result of existence of hard parts of bone.
    Keywords: Chemistry ; Fisheries ; Iran ; Precooking ; Filling media ; Heating regime ; Sterilization ; Silver carp ; Mineral elements ; Hypopthalmichthys molitrix ; Samples ; Sunflower oil ; Soybean oil ; Silver Carp
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25438 | 18721 | 2018-09-23 16:51:04 | 25438 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Caspian trout is valuable, Commercial, anadromous and endemic fish species in the Caspian Sea so that, researchers attention have been focused on this species. At the moment, only pay attention to stocks rehabilitation that fisheries organization releases more than 300,000 fingerlings to the rivers ends to the Caspian Sea. Importance of this study consist (1) introducing a new species to the aquaculture system and (2) provide breeders in this research. we used from 2 treatments that consist with density of 10 kg/m^3 and 20 kg/m3 with 3 replication. Results showed, culture of Caspian trout is suitable with density 10 kg/m^3, so that weight gain, SGR and Daily growth mean are better than treatment 2(15-20 kg/m^3 )
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Salmo trutta caspius ; Growth factors ; Feasibility ; Culture ; Brackish water
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25452 | 18721 | 2018-09-26 09:35:53 | 25452 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: At the present study, the water quality parameters monthly, environmental pollutants (pesticides, heavy metals and surfactants) seasonally, macrobenthos and bacteria (monthly) were done 2009 t0 2010 that located in Mangol Dam with longitude and latitude 52° 22' 49" N and 43° 15' 43" E, respectively. All water samples were collected from under-groundwater, surface water and wastewater in 12 stations and the results are as follow: The mean concentrations of DO, BOD_5, COD, HCO^3-, CO_3^2-, TA, Ca^2+, Mg^2+, TH, TSS, TDS, Cl^-, PO4^3-, NH^4+, NO^2- and NO^3- were determined about 11.86±0.49, 5.95±0.11, 4.96±0.59, 28.1±6.1, 14.34±1.64, 36.30±2.33, 64.66±2.34, 28.84±1.52, 271.62±8.41, 0.37±0.03, 0.45±0.02, 28.02±1.96, 0.06±0.01, 0.010±0.001, 0.01±0.001 and 0.96±0.03 mg/l, respectively and also temperature, pH and EC were 12.7±0.61°C, 8.00±0.02 unit, 0.57±0.02 ms/cm in all samples. The surface water quality of Haraz River and its tributaries were suitable for usual applications such as aquatic matters, but based on BOD_5 need to more attention for human usages. The water quality index (WQI) of surface water was classified in group II at all stations that shows serious water quality changes due to domestic and agriculture sewage. The groundwater was affected by different matters that caused change of water quality parameters. The wastewater was classified from good to moderate based on BOD5. The quality of wastewater was declined at down-stream with decreasing DO and pH and increasing BOD5 pollutants. The mean concentrations of aldrin and endrin were about 0.66 ± 0.59 and 0.71 ± 1.07 µg/l, respectively. The maximum seasonal concentration of organochlorin pesticides (OCPs) were detected in fall, winter and spring for endosulfan sulfate, γ-BHC, heptachlor, endrin aldehyde compounds about 2.85, 0.34, 0.14 and 0.14µg/l, respectively. The maximum seasonal percentage range of OCPs in fall, winter and spring for endosulfan sulfate, DDD and β-BHC were 42, 25 and 25 %, respectively. The maximum seasonal concentrations during fall, winter and spring were detected in Sorkhroud and Polechelave stations for only Fe about 1.48, 3.3 and 8.22 µg/l, respectively. Also for Hg in spring was determined about 17.2 mg/l. The mean concentration of Zn, Fe and Hg in water sampled were 0.23 ± 1.01 and 1.21 ± 2.00 µg/l, respectively and 4.65± 6.38 mg/l. The maximum concentration of linear alkyl benzene sulfonate (LAS) in summer was 971µg/l. The mean concentration of surfactant during fall, winter, spring and summer was 418.62 ± 52.16 µg/l. The maximum seasonal percentage ranges of surfactant during fall, winter, spring and summer in all stations of sampling were 76, 76, 100 and 100 %, respectively. Tottaly 15 families of macrobenthos were identified in Haraz River. The maximum mean of abundance and biomass in Sorkhroud and Nour-roud stations were 1177±256 ind/m^2 and 3609±935mg/m^2, respectively. During Seasons, the maximum mean abundance and biomass were detected in fall and winter with 1185±444 ind/m^2 and 3891±849 mg/m^2, respectively. Water quality were classified by Hilsenhoff Biotic Index such as Sorkhrood station as "Very Poor", Isar town station as "Fairly Poor" and Karesang and Nor-roud stations as "good" qualification. The maximum of total coli forms and fecal coli forms in surface water were observed in Sorkhrood about 4.9 and 2.3CFU/100ml, respectively. The maximum of total coli forms and fecal coli forms in groundwater were observed in Halomsar and Absak stations about 4 and 1.7CFU/100ml, respectively. Maximum of total coli forms and fecal coli forms in wastewater were observed in Nor-roud station about 3.1 and 1.6CFU/100ml, respectively. The Ovary of nematode in wastewater were observed in Nor-roud and Isar Town stations about 30 through 124, respectively.
    Keywords: Ecology ; Iran ; Haraz River ; Mazandaran province ; Mangol Dam basin ; Physico-chemical characteristics ; Surface water ; Ground Water ; Wastewater ; Pesticides ; Heavy Metals ; Surfactants ; Macrobenthos ; Coliforms ; Nematodes ; Environmental pollutants ; Bacterial ; Temperature ; pH ; Biomass
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25443 | 18721 | 2018-09-25 18:03:13 | 25443 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The culture of Iranian Shrimps began in Bushehr in 1372. Early the green or the pink tiger shrimp (Penaeus semisulcatus) which were found in most habitats of the Persian Gulf and Oman Sea were considered in breeding and reproduction, but due to reproduction difficulties, it was rather replaced by the Monodun imported species, then the production of the white shrimp Metapenaeus affinis and Then P.merguensis in some southern areas of the country such as Hormozgan has began in small scale,but none of these species couldn't meet the economical needs of the consumer society and they were not indelible and dominant enough in the shrimp aquaculture industry of the country until breeding of Indian White Shrimp (Fenneropenaeus indicus) has began in large scale in the provinces of Khuzestan and Bushehr, which are seen as the heart of shrimps reproduction and breeding, and in Hormozgan and Sistan. the incidence of white spot viral syndrome (White Spot Syndrom Virus) led to the import of the western white leg shrimp from the USA (P.vannameii) by the Iranian Fisheries Research Organization (IFRO) in 1383, at the present all southern and northern breeding farms of the country (fundamental measures has been carried out in line of shrimp reproduction in Gorgan province in 1386) has put the breeding of this shrimp species as the single breeding species at top of their agenda. The subject of feeding the breeding shrimps is widespread and regarding to the non-proprietary species there are still some species which contain high protein than the other species in studying the different shrimp species requirements. In Iran due to the scarce factories producing the shrimp's food, the alimentary compound of the breeding shrimps during industrializing years of this type of breeding even after the non-aboriginal western white leg shrimp species being inclusive was stable and through different biological processes including: Naplies which feeds from its yalk and by entry into zoa stage start eating only small-sized phytoplankton. And by entry into the stage between zoa and mysis it feeds from phyto and zooplankton simultaneously and entering the post-larva stage it stars sarcophagi. and after 15 post-larva stage entering the growth and transition stage to earthen ponds feeding by concentrated industrial foods in large and small packages depending on young and adult mouth and due to feeding requirements of every stage the alimentary compound is nearly as follows: Digestible protein, energy, fat, carbohydrates, vitamins and minerals will start and the final product will be released in market Or they will be used in the later years of laying eggs and feeding for brood stock. In feeding section the details of every stage and their food's nutritional needs at each stage and will be fully described.
    Keywords: Aquaculture ; Iran ; Persian Gulf ; Oman Sea ; Hormozgan Province ; Bushehr Province ; Shrimp ; Food components ; Biological processes ; Nutrition ; Feeding ; Culture ; Tiger shrimp ; Penaeus Semisulcatus ; Breeding ; White shrimp ; Metapenaeus affinis ; Aquaculture ; White Spot Syndrome Virus ; Phytoplankton ; Zooplankton
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25448 | 18721 | 2018-09-23 17:20:27 | 25448 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The study was conducted to achieve growth, survival and reproductive characteristics of four Artemia populations (A.urmiana , A.franciscana , Pakistan strain and Turkmanestan strain). In this study, The strains were cultured under the same and static environment in laboratory condition. All cysts strains were hatched using the standard methods and the nauplii from the populations were cultured in laboratory condition using 80 g/L salinity, 25±1ºC with photoperiod (12L:12D) and Dunalliella tertiolecta were fed. The growth and survival of artemia were determined on days 8, 11, 14, 17 and 20 of culture. Randomly, The adult population were placed in 50 ml conical falcons and reproductive characteristics were determined. The results revealed that the survivorship A. franciscana had the highest survival (%99.8) and A. turkmanestan lowest survival (%92.1) The results revealed that the in growth rate while pakistanian strain had the highest growth rate(8554 micron). The results revealed that the reproductive characteristics in each population of species , the highest cyst production were observed in A. urmiana, A. franciscana and A. turkmanestan strains and the highest nauplii production was due to pakistanian strain. The highest production were observed in A. franciscana and lowest production were observed in turkmanestan strain.
    Keywords: Health ; Iran ; Pakistan ; Turkmanestan ; Artemia ; Strain ; Survival ; Growth ; Reproduction ; Population ; A.urmiana ; A.franciscana ; Culture ; Dunalliella tertiolecta
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25173 | 18721 | 2018-09-03 17:10:58 | 25173 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Low hatching artemia cysts which has no use in the aquaculture can be decapsulated and directly use in larvae culture. In order to increase the knowledge of consumers to know the quality changes of low hatching decapsulated cysts during storage after production (especially fatty acids) this research was carried out. In this work Low hatching cysts of artemia urmiana had decapsulated with sodium hypochlorite and other chemicals. We used drying room and f.b.d system for drying of this decapsulated cysts and package units had used for packing this product to cans. We had three experimental treatments of dry decapsulated cysts (3 type packing including vaccumed cans and free (plastic dishes). These treatment were evaluated at different times (comparing of before produce, after produce , 3, 6 and 12 months keeping after production ) such as fatty acids , fatty percent , protein percent , bacterial and fungal contamination and presence of aflatoxin the result of fatty acids using one way anova analysis showed that the variation of half of fatty acids specially DHA and EPA were significant (p〈0.05) . Fatty percentage increased and protein was decreased about 12-15% during the one year storage we separated two species of bacteria and two species of fungi but they had no pathogenic bacterial and fungal. During 6 months storage the quality of products have less variation compared whit 12 months.
    Keywords: Aquaculture ; Iran ; Non- Atchabledecapsulated ; Cyst ; Artemia Urmiana ; Larvae ; Culture ; Fatty acids ; Protein ; Bacterial ; Fungal ; ANOVA ; Species ; Pathogenic ; Quality ; Evaluation ; Hatchable ; Aquaculture
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25175 | 18721 | 2018-09-03 14:16:47 | 25175 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: High mortality frequently occurs in larval mass production of Persian sturgeon fish, Acipenser persicus. Nutritional deficiencies in live food, Artemia urmiana nauplii, fed to larvae could be a reason. A series of experiments was carried out to evaluate the effects of nutritional enrichment of Artemia by oils in different concentrations with or without different levels of vitamin C during two enrichment periods (12 and 24h) on survival, growth rate and salinity tolerance in Persian sturgeon larvae reared in tanks after 20 days. Four types of oils (ICES30/4, sturgeon ovary, cod liver, and linseed oils) were evaluated. HUFA oil source with or without vitamin C did not affect the growth rate of Persian sturgeon larvae. Nevertheless, survival rates and chemical compositions were significantly improved (P〈0.05) only in fish larvae fed Artemia enriched HUFA oil supplemented with vitamin C. ICES30/4 was the best oil source for improving the chemical composition including fatty acids in Persian sturgeon larvae only when it was supplemented with vitamin C. Lower levels of vitamin C (less than 30%) and longer enrichment period (24h) were more effective (P〈0.05) in improving on chemical composition of sturgeon fish larvae. When the sturgeon larvae were exposed to salinity test, those fed with HUFA enriched Artemia showed tolerance only up to 6 ppt while those fed HUFA+vitamin C enriched Artemia demonstrated high tolerance (≥ 90% survival) up to 12 ppt for 120h. Although the enrichment of Artemia urmiana with 300 ppm ICES30/4 and 30% vitamin C gave the best salinity tolerance at 12ppt (100% survival), the enrichment with 300 ppm sturgeon ovary oil and 10% vitamin C was economically affective in increasing the salinity tolerance of Persian sturgeon larvae (98 % survival).
    Keywords: Aquaculture ; Iran ; Survey ; Artemia urmiana ; Vitamin C ; HUFA ; Daphnia ; Enrichment ; Persian sturgeon ; Acipenser persicus ; Chemical composition ; Salinity Tolerance ; Survival ; Resistance ; Larvae ; Huso huso ; Nauplii ; Feed ; Food ; Growth ; Sturgeon larvae
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25526 | 18721 | 2018-10-05 15:47:47 | 25526 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was to assess the silver carp diet compositionduring grow out period. The experiment was conducted in a well-managed fish farm located at Mazndarn province from 2012 to 2013. Totally five samples from the pond water and also silver cap intestines were collected in May, April, Jun, July, August and September of 2012. The results showed that there are five phyla of phytoplankton in the ponds including: Phyrophyta, Bacilluryophyta, Cyanophyta, Clorophyta, Oglenophyta. A comparison of phytoplankton populationindicated a significant differences throughout the five sampling sessions (P〈0.05). Phytoplankton population increased gradually from April to September. The biomass of the phytoplankton was not also similar during the experiment (P〈0.05) and this value elevated along with increasing the phytoplankton number from April to September. The overall intestine content demonstrated that around 39.2% of the proximal intestine content composed ofChlorophyta . However Chlorophyta concentration reached to 34.9% for distal part of the intestine. The rest of silver carp intestinal phytoplankton were Bacilluriaphyta ,Cianophyta, Oglana and Phyrophyta, respectively.
    Keywords: Aquaculture ; Iran ; Mazandaran province ; Silver Carp ; Feeding ; Chlorophyta ; Bacilluriaphyta ; Cianophyta ; Oglana ; Phyrophyta ; Survey ; Hypophathalmichthys molitrix ; Samples ; Cyanophyta ; Oglenophyta ; Phytoplankton
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25538 | 18721 | 2018-10-05 15:45:55 | 25538 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This survey carried out from 2012 to 2013 to study of suitable site selection for carp and trout fish species for responsible aquaculture possibility by data collection and literature study. During the survey ecological factors including physical and chemical factors, geological factors including topography and type of soil, biological factors including selection of species and control of invader animals, climatic factors, geographical factors and pollutant including heavy metals, agricultural pesticides Surfactants and crude oils were recognized. Also in comparing with standard levels suitable rate of each important factor in aquaculture were studied.
    Keywords: Aquaculture ; Iran ; Warm Water ; Fish ; Cold Water ; Ecological factors ; Biological factors ; Aquaculture ; Survey ; Carp ; Species
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25578 | 18721 | 2018-10-06 17:39:33 | 25578 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In the present study, the possibility of marine culture of Asian sea bass in the shrimp earthen ponds located in the Gwadar region (coastal area of Oman Sea, southeastern of Iran) was investigated. To this end, Asian sea bass frys reared for 7 months including 1 month nursery and 6 months growth-period. During the culturing periods, fish were feed ad libitum by various commercial rainbow trout diets based on fish growth stages. After nursery period, the average weight of 11.05±1.5 g and survival percent of 65 % were obtained for fish. During the growth-out period, the average weight increased continually from 31.4±4.7 g in late June to 252.1±30 g at the end of growth-out period in late Dec. Also, the highest values of monthly weight gain yielded during August, Sept and Oct when fish had the average weight range of (76.9±14.4)-(188.1±12.8) g. The values of monthly feed conversion ratio (FCR) were low and similar from July to Oct and then increased in Nov and Dec. The values of total monthly feed intake (FI) increased continually from July to Oct and then with a decrease in Nov, increased again in Dec. The values of survival percent after nursery and growth-out period were 65 % and 66% respectively. Also, the average of monthly FCR, FCR at the time of fish harvesting, total FI and total harvested biomass were 1.36, 2.5, 630.5 kg and 325 kg. in conclusion, the pond culture of Asian sea bass with marine water results appropriate survival and growth rates so that fish (11.05±1.5 g) obtained marketable weight (252.1±30 g) during 6 months culturing period. It seems that first-half of year in Gwadar area is more suitable for sea bass culture since this time periods results better FCR ( fed by rainbow trout feed) and has economic justification. Also, with regular sorting operation and providing of species-specific feed for Asian sea bass can increase the Production per Unit Area.
    Keywords: Aquaculture ; Iran ; Gwatr ; Sistan and Baloochestan ; Chabahar ; Lates calcarifer ; Asian sea bass ; Culture ; Pond ; Lates calcarifer ; Rainbow trout ; Survival rate ; FCR
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25570 | 18721 | 2018-10-06 11:30:10 | 25570 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The aim of this study was comparison the effect of cow dung as organic fertilizer and chemical fertilizer on microbial flora, fish and human pathogenic bacteria and physico-chemical parameters of cyprinus fish pond water. The water samples were collected from two ponds, pond 1 was fertilized by cow dung and pond 2 was fertilized by chemical fertilizer, respectively from May to October 2011. . The aerobic and anaerobic bacteria were enumerated in TSA by serial dilution of the sample, followed by conventional pure plate method. Coliforms bacteria were similarly isolated on Chrom agarTM ECC. Moreover, the water samples were used to analysis of BOD, dissolved oxygen, conductivity, transparency, total alkalinity, total dissolved solid, total hardness, total nitrogen and total phosphorous. Temperature and pH were measured by thermometer and portable pH meter in place of ponds. For isolating of fish and human pathogenic bacteria, thirty six fish were collected from pond 1 from July to October and then sampled from their liver and kidney and inoculated in blood agar. The mean of total count of bacteria and total coliform count in cow dung sample were 4 × 104 ± 1200 and 4700 ± 300 CFUmL^-1 respectively. The mean of water total count bacteria of pond 1 were significantly higher than the water of pond 2. The water total count bacteria of pond 1 were significantly increased in August, but water total count of pond 2 was not shown significant variation from May to October. The results showed that Pseudomonas, Klebsiella, Enterobacter, Yersinia and E.coli were isolated from water of pond 1 but just Pseudomonas and E.coli were isolated from water of pond 2. Moreover, the human pathogenic bacteria shuc as Shigella and Salmonella were not isolated from of water and fish samples from pond 1. In this study, the bacteria growths were negative in all of the inoculated plate from fish liver and kidney. The total hardness, total phosphorus, total dissolved solid and conductivity of the pond 1 water were significantly higher than the pond 2 water. But the BOD, dissolved oxygen and transparency of the pond 2 were significantly higher than the pond 1 water. There were no significant differences in the total nitrogen, total alkalinity and pH between water of pond 1 and 2. In recent years, the chemical fertilizer has been used in cyprinus fish pond but many studies have showed that they were high risked as environmental pollution. Thus, the use of livestock manure especially cow dung could be a suitable alternative. The results showed the use of cow dung increased the bacterial population and diversity. Increasing of bacteria population caused increased total phosphorus that plays an important role in bloom of plankton because we found decreasing transparency and planktons are an important food for cyprinus fish. In despite of isolation of fish and human pathogenic bacteria from water, the bacteria growths were negative in all of the inoculated plate from fish liver and kidney. In summary, it seems the cow dung is a suitable alternative for fertilizing of cyprinus fish pond water if a microbial and physico-chemical monitoring of fish and pond water were continuously arranged.
    Keywords: Aquaculture ; Health ; Iran ; Pathogenic ; Bacteria ; Cyprinid ; Cow dung ; Fertilizer ; Microbial flora ; Cyprinus fish ; Dissolved oxygen ; Transparency ; Alkalinity ; Temperature ; pH ; Kidney ; Pseudomonas ; E.coli
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  • 65
    Publication Date: 2021-07-16
    Description: The national research plan with title of "Study on health status of Iran Coldwater hatchery and rearing farms" was done in order to identification and tracking of main causative agents of recent mortality in Iran Coldwater hatchery and rearing farms and recognize of infected zones and design of landscape distribution of Epizootic Coldwater fish diseases in the country. This plan was conducted parallel and in same time in some polar provinces of Coldwater fish production such as Mazandaran, Gilan, Ardebil, West Azerbaijan, East Azerbaijan and Kurdistan in three years from 2006-2008. In this regard, about 23,21 and 20 farms in Mazandaran province, 30 farms in West and East Azerbaijan and Kurdistan provinces and 19 farms in Gilan and Ardebil were selected as randomly and tracking were done yearly from 2006-2008. This research was conducted according to regular planning consists of farms visit that was done according to statistical plan and completion of Questionnaires and sampling. The findings in Mazandaran province revealed that fish infections background and infectious diseases were increased in period of 2006-2008. In fact, 56% of all fish farms in 2006, 71% in 2007 and 85% in 2008 were recorded as infected farms. In other side, average weight of fish final products was more 500gr in consume market. So, regarding to long period of fish culture in mentioned fish occurrence of more morbidity could be expected. The streptococcus infections were most important fish bacterial diseases that have more incidence and pathogenicity in collected questionnaires. Also it revealed more occurrences in summer season in above 15oC temperature in affected fish farms. Also, Enteric Redmouth Disease (ERM) and Saprolegniasis were reported as second and third degree in examined farms. Meanwhile, findings of control, prevention and treatment of our survey revealed that using of antibiotics and detergent materials were increased in mentioned province. Indeed, 34.7% of all fish farms in 2006, 71.4% in 2007 and 75% in 2008 have applied treatment operation and using of detergent materials in affected farms. In other side, frequent using of Erythromycin antibiotic was reported several times in 2008 so fish farmer faced to Bacterial resistance and should be using from Florfenicol as alternative antibacterial. Also, in feed sampling from all fish farms about 3 fish farms in 2006 and 5 fish farms in 2008 were faced with food poisoning that originated from high TVN and peroxidase in fish food consumed. These farms revealed mass mortality that stopped after change feeding regime. In conclusion absence of fish health management could be considered as main agent of mentioned mortality in Mazandaran province. The findings of our survey in West and East Azerbaijan and Kurdistan revealed that absence of sufficient experiences in fish farmers and their neglect from water quality concepts, farm cleanness, using of suitable detergent materials for fish ponds and instruments and disability in continuous monitoring of physic-chemical factors of consumed water could be considered as most important problems. So, fish morbidity and mortality, growth decrement and low production rate were expected. Unfortunately, despite the occurrence of infectious and non- infectious diseases in examined fish farms, there were no documents and information about diseases history, clinical signs in dead fish, feeding regime, fish mortality (rate of daily mortality, age and weight of moribund fish), treatment operation, drugs usage, growth rate, physico-chemical factors and fish density. So planning for control and prevention of mentioned diseases were unsuccessful in affected fish farms. Also, occurrence of some epizootic fish viral diseases such as VHS, IPN and IHN were observed frequently with mass mortality about 40100% in some examined fish farms that produced some tragic economic lost in the mentioned provinces. Similarly, some bacterial, fungal and parasitic diseases were observed repeatedly in some inspected farms such as Flavobacterium, streptococcus infections. Also, some unknown causative agents in parasitic diseases were observed several times such as Dactylogyrus, Gyrodactylus, Ichthyophthirius multifiliis, Trichodina, Chilodonella, Diplostomum and fungal diseases such as Saprolegniasis. In conclusion it seems that absence of fish health management, inadequate rate of hygienic technical services, absence of quarantine programs for transfer of new eyed-eggs, juvenile and live broodstocks packages without health certificates from authorized organizations, uncontrolled entry of foreign eyed-eggs (France, Denmark, Australia and Armenia) and neglect in using of suitable detergent materials for fish ponds, instruments, consumed water and infected eggs, not screening of broodstocks in hatcheries could be considered as most important causative agents in occurrence of infectious diseases and main problems in affected farms in mentioned provinces. Similarly, our findings in Gilan and Ardebil provinces revealed that their problems were similar to other provinces. In fact, neglect in establish of necessary Infrastructures in fish farms and dereliction in health management concepts could be considered as main reasons of occurrence of fish infectious and non-infectious diseases in studied fish farms in mentioned provinces. In fact, often fish farms visited hadn't suitable structures without control and prevention approach. There were not observed detergent using, quarantine programs and prevention methods. Entrance of unknown persons, birds and wild animals were ordinary in mentioned farms. Unfortunately level of farmer’s knowledge was low and no training courses and extension programs were planned by authorized organizations. Meanwhile, food storages were unsuitable and food packages were stored in inappropriate situation so poisoning conditions were increased in examined farms. In conclusion it could be finalized that Coldwater fish farms in Gilan and Ardebil provinces situated in insufficient position and correction of current situation are needed urgently. In final elicitation, it would be mentioned that lack of basic infrastructures could be introduced as most important reasonfor spread of diseases, mortality and related economic losses in studied provinces. Therefore, attention to environmental affairs and access to Sustainable Development are recommended. Also consideration of biosecurity regulations and health management concepts would be important requirements for modification and reformation of Coldwater fish farms in examined provinces towards A Better Tomorrow.
    Keywords: Aquaculture ; Iran ; Mazandaran Province ; Gilan province ; Ardebil Province ; West Azerbaiejan Province ; East Azerbaiejan Province ; Kordestan provinceColdwater ; Fish farm ; Health situation ; Health management ; Diseases ; Survey ; Rearing ; Hatcheries ; Enteric redmouth disease ; Saprolegniasis ; Bacterial ; Florfenicol ; Sampling ; Dactylogyrus ; Gyrodactylus ; Ichthyophthirius multifiliis
    Repository Name: AquaDocs
    Type: monograph
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  • 66
    Publication Date: 2021-07-16
    Description: This study have been conducted entitle biological and statistical sturgeon in the southern Caspian Sea (Mazandaran waters)” from 2009 to 2013. Sampling was manthly. Overal, 1859 specimens of different species of sturgeon was caught including Acipenserpersicus (69%), A. stellatus (19%), Husohuso (6%), A. nudiventris (4%) and A. guldenstaedtii (2%). Totally, the meat and caviar of five species were found 53263.6 kg and 5633 kg respectively and caviar to meat ratio was 10.6%. The results show that ration of meat and caviar whole species reduced from 2009 to 2012 in which the meat and caviar of A. persicus was declined from 12146 to 4465 kg in meat and caviar from 1358 to 487 kg were extremely reduced respectively. The CPUE (catch per unit effort) of sturgeon has fluctuated from 2009 to 2012. Totally, the CPUE of A. persicus was decreased from 0.150 (2009) to 0.130 (2012) kg boat per day, A. stellatue from 0.009 (2009) to 0.021 (2012) kg boat/day, A. guldenstaedtii from 0.002 (2009) to 0.003 (2012) kg boat/day, A. nudiventris from 0.027 (2009) to 0.001 (2012) kg boat/day and H. huso from 0.123 (2009) to 0.018 (2012) kg boat /day. 1061 specimens of fish has been caviar including A. persicus with 69%, A. stellatus (18.1%), H. huso (5.5%), A. guldenstaedtii (2.7%). The frequency of caviar categorize was included 1 , 2, 3 and massive with 49.6%, 38%), 10.6% and 1.9% , respectively. The highest of meat and caviar belong to A. persicus with 31414 kg and 3515.7 kg, respectively were found and the lowest was 841kg and 105.9 kg pertain to A. guldenstaedtii. The results show that 65.9% of sturgeon was caught by legal catch (landing) and 34.1% by beach seine remained. 391 individuals was age determined that the minimum and maximum age was 9 and 41 year comprised to H.huso and A. stellatus, respectively. A. persicus and A. stellatus were majority caught compare to other species. A. persicus has the highest frequency in 14-15 year age groups with 50% and 57% in 2009 and 2011, respectively and 15-16 year age group with 41% and 56% in 2010 and 2012, respectively. The results show that mean of fork length, body weight, caviar weight of A. persicus was declined because the FL and weight average (±SD) obtained from 152.4±16.7cm and 25.8±9.2 kg in 2009 to 146.5± 18.7cm and 22.1±9.2kg in 2012, respectively. In contrast, the average (±SD) FL and weight of H. huso was increased from 218.8±41.1cm and 137.3±96.6kg in 2009 to 231.3±45.2cm in FL and 146.2±78.5kg body weight in 2012, respectively. Consequently, the results show that stocks of sturgeon in the Caspian Sea has a trend declined because of some species in IUCN categorize list was Endangered in 2006 but at this moment lie in Critically Endangered list of IUCN categorize were down which could be a serious alarm for the valuable sturgeon fish in the Caspian Sea. Therefore, recommendation that trade catch of sturgeon in the Caspian Sea must be forbidden and continue legal catch for spawner fish that needede to artificial propagation only.
    Keywords: Biology ; Ecology ; Iran ; Caspian Sea ; Mazandaran waters ; Biology ; Sturgeons ; A. persicus ; A. stellatus ; A. guldenstaedtii ; A. nudiventris ; H. huso ; CPUE ; Survey ; Sampling ; Species ; Caviar
    Repository Name: AquaDocs
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  • 67
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25587 | 18721 | 2018-10-07 11:26:14 | 25587 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This project was conducted in imam Khomeini mariculture research state from October 2010 to June 2013 to compare reproductive performance between wild and domesticated Sobaity seabream broodstocks. In 3 years wild broodstocks did not spawn in 5 and 10 fiberglass and concrete tanks but domesticated broodstocks spontaneously spawned from February to March. In the first year domesticated broodstock in ratio of 3 females to 6 males allotted in 10 mt concrete tanks in triplicate. the about 2776000 eggs produced from 9 females and 18 males but further heavy mortality occurred because of winter shock syndrome and suppression of immunity system. Mean temperature, salinity and PH in incubation period was 16.5˚C (range between16-18˚C), 45ppt, 8.5 respectively. Mean floating eggs and hatch percent was 15.5% and 8.9% and about 19000 larvae produced. In the second year domesticated broodstock in ratio of 9 females to 23 males allotted in 5 mt fiberglass tanks in triplicate .about 17540000 eggs acquired from 27 female and 69 male broodstocks. Mean temperature, salinity and PH in culture period was 24.8 ˚C(range between20-28.3˚C), 47.5 ppt, 8.4 respectively. Mean floating eggs and hatch percent was 58.94% and 84% and about 8640000 larvae produced and stocking density was 10-30 larvae per liter. After 61 days mean production and survival were 503.3 (range between 140-1220) larvae per cubic meter and 5.3% (range between 0.47–12.2 %) respectively. Final mean weight of fry was 2.6 gr. In the third year domesticated broodstock in ratio of 3 females to 6 males allotted in 5 mt fiberglass tanks in triplicate. about 6570000 eggs acquired from 9 female and 18 male brood stocks. Mean temperature, salinity and pH in culture period was 23.9˚C(range between 19.5-28.4˚C), 45, 7.6 respectively. Mean floating eggs and hatch percent was 54.7% and 81.6% and about 2889800 larvae produced and stocking density was 10-30 larvae per liter. At the end of 87 and 89 days of husbandry the mean production and survival were 359 (range from 300 to 397) larvae per cubic meter and 2.7% (range between 1.32–3.8 %) respectively. Final mean weight of fry was 3.5 gram and Larvaeculture in the last 2 years carried out in 5 cubic meters fiberglass tanks. It seems to domesticated broodstocks because of spontaneous spawning, long spawning period, more brood stocks and possibility of reusing them in the next year are very economical for propagation than wild broodstocks.
    Keywords: Aquaculture ; Iran ; Sparidentex hasta ; Fry production ; Spawning ; Larvae culture ; Propagation ; Sobaity ; Sparidentex hasta ; Rearing ; Mariculture ; Broodstocks ; Temperature ; Salinity ; pH ; Survival
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  • 68
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25631 | 18721 | 2018-10-07 16:55:18 | 25631 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Rutilus kutum is one of the most important economic fish species of Iranian costal of Caspian Sea. This species conducted more than 60% of fish caught in Caspian Sea annually, and more than 10 thousands fisherman engaged to its fishery. In present study, kutum fingerlings reared in two different densities include of 25 and 40 fish/m^2 with mean weight of 17.5±5.87 g. The period of test being planned 10 weeks and the test organism housed in circular concrete pools of 3 m^3 capacity with flowing water and aeration in pools are supplied. The fishes feed by extrude food with 45% crude protein and 14% crude fat. After termination of the experiment, the result showed that weight gain performance, specific growth rate, condition factor, food efficiency rate and protein efficiency rate decreased, while food conversion ratio increased with rising of density, as their different was significance in two experimental treatments (p〈0.05). The kutum reared in low density showed significantly higher survival rate (SVR) (with 88.8%) than high density (with 76.9%) (p〈0.05). Based on the different observed effects of the density on growth and survival, it is recommended the use of a 25 kutum/m^2 density for rearing by Caspian Sea water.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Rutilus kutum ; Density ; Culture ; Growth ; Survival ; Fishery ; Fingerlings ; SVR ; Rearing
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  • 69
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25633 | 18721 | 2018-10-07 16:59:44 | 25633 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Streptococcosis is an infectious bacterial disease that causes huge economic losses in cold water aquaculture industry. Disease outbreak was experienced in some of provinces farms in recent years. Fars Province, has produced 7,000 tons of cold-water fish. According to Streptococcosis report in 1381 from the province and Proceedings have been performed during 10 years against disease and also economic losses impact of disease on rainbow trout production, risk assessment of Streptococcosis conducted on the plan. In this study, of 586 sick fish (have symptoms) studied 230 fish (39.24%) Streptococcus and (26.62%) gram negative bacteria were isolated. Of 754 healthy grower fish and fry (with no clinical signs) 10 fish or fry (1.32%)infected with streptococcus and 60 fish or fry(7.95%) infected with gram negative bacteria (Yersinia ruckeri, Pseudomonas, entrobacteriaceae). According to biochemical tests and molecular examinations, isolated Streptococcuus iniae and Streptococcus sp. Furthermore some physical and chemical parameters measured and aerobic bacteria of selected farm water counted. These factors effects on disease incidence and changes were evaluated by applying logistic regression.
    Keywords: Aquaculture ; Health ; Iran ; Fars province ; Streptococcosis ; Risk factors ; Rainbow trout ; Survey ; Diseases ; Fry ; Clinical signs ; Bacteria ; Yersinia ruckeri ; Pseudomonas ; Entrobacteriaceae
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  • 70
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25634 | 18721 | 2018-10-14 02:42:53 | 25634 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The influence of stocking density was investigated on growth, survival and FCR of great sturgeon (Huso huso) juvenile in north of Khuzestan province (Dezful). The study was conducted by Southern Iran Aquaculture research center. This study was done from September 2013 to July 2014 in three stage with different weight of Huso huso juveniles. In the first stage density of 1.5, 3 and 6 kg/m^2 during two month was performed. The initial weight and length of fish in this stage considered as 143±0.29 g and 316±1.1 mm. In the second stage 3, 6 and 9 kg/m2 density were introduced. The initial weight and length of fish in this stage considered as 548.58±33.26 g and 416.5±0.81 mm and duration of the stage was 84 days. In the third stage 6, 9 and 12 kg/m^2 density were introduced and initial weight and length were 1571.33±71.33 g and 687.5±10.96 mm. Growth and feeding index were assess in each stage. In the first stage monthly blood samples were collected from each density. In this stage bold and hormonal indices were assessed. The results showed that the average final weight in stage 1 in 1.5, 3 and 6 kg/m^2 densities were 527.27±6.82 g, 467.91±18.81 g and 431.02±24.25 g respectively. The highest average of weight was in treatment 1 and it has significant difference with treatment 3 (P〈0.05). FCR was lower than 0.7 in every treatment. FCR, condition factor, daily growth, special growth index, BWI and food performance index have not significant difference in treatments. In the second stage, average final weight of Huso huso was 1734.91±97.65g, 1525.88±57.20 g and 1370.95±1593 g respectively. The highest average weight was in treatment 1 and has significant difference with treatment 2 (P〈0.05) FCR in treatment 1, 2 and 3 were 0.92±0.2, 0.96±0.26 and 1.11±0.36 respectively and there is no significant difference between them (P〉0.05). FCR, condition factor, daily growth, special growth index, BWI and food performance index have not significant difference in treatments. Daily growth rate in treatment 1, 2 and 3 were 13.93±2.83, 11.44±2.65 and 9.6±2.49 respectively. In the third stage the average final weight in 6, 9 and 12 kg/m^2 densities were 3232.22±66.12g, 3407.39±119.73g and 2869.72±135.7g. The highest average weight was in treatment 2 and has significant difference with treatment 3 (P〈0.05). FCR in treatment 1, 2 and 3 were 1.19±0.2, 1.25±0.1 and 1.45±0.27 respectively and there is no significant difference between them (P〉0.05). FCR, condition factor, daily growth, special growth index, BWI and food performance index have not significant difference in treatments. Daily growth rate in treatment 1, 2 and 3 were 20.37±3.12 g/day, 19.90±4.2 g/day and 16.36±3.64 g/day respectively.
    Keywords: Aquaculture ; Iran ; Khuzestan provience ; Survey ; Bluga ; Huso huso ; Sturgeon ; Density
    Repository Name: AquaDocs
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  • 71
    Publication Date: 2021-07-16
    Description: This study aimed to investigate the effects of 5- parameter dissolved oxygen, PH, nitrite, nitrate and temperature of the water on Streptococcosis incidence in two groups of fry and grower fish. Research was performed at west Mazandaran province -Tonkabon region in 12 rainbow trout selected farms. Research conducted in 3 farms with well source water, 8 farms with water source of the river of Dohezar and one farm with water source of the river of Azarood, during 12 consecutive months. From 1390.04.01 to 1391.04.01, once time each month, and in each time 10 fish randomly sampled, inlet water were sampled simultaneously Of 1350 sampled fish 607 fish with an average weight 22.04 gr, average length 12.59 cm were in fry category and 743 fish with an average weight 156.25 gr, average length 23.32 cm in were grower category. Streptococcosis observed only in grower category Of 72 fish with clinical signs of the disease, 14 numbers were positive Streptococcosis (19.44%) and 58 numbers were negative Streptococcosis (80.55%). Three fish from grower category has not any clinical signs of disease and seemed to be healthy but were positive Streptococcosis in examinations (0.22% of total fish sampled. Fish with clinical signs of the disease but negative Streptococcosis were of at least 55.1 gr and at most weight 417 gr respectively. The results showed that 47.07% cases of Streptococcosis happened at 15.6°C water temperature, 35.29% at 16.98°C and 17.64% cases happened at 18.04°C so that 100% of Streptococcosis cases was observed at the average temperature of 16.99°C. In addition, the survey results show that despite relatively high levels of nitrite in source water of farms from wells, nitrite does not effect on the disease incidence. It seems that an optimal level of dissolved oxygen in water is effective in reducing the effectiveness of nitrite in this disease. According to equation coefficients logit model is as follows: According to Logit model, it seems that for every degree change in temperature and pH of water, morbidity change will diminish 0.37 % and 1.74 % respectively. 80.56% of fish sampled that had the clinical symptoms, was ranging from an average weight of 5.1 gr (fry) and 417 gr (grower fish), that despite having clinical signs of the disease were negative Streptococcosis. Isolation of Staphylococcus bacteria as well as Gram-negative bacilli from fish with clinical symptoms similar to the symptoms of Streptococcosis, may indicate the involvement of other pathogens in fish clinical signs.
    Keywords: Ecology ; Iran ; Mazandaran Province ; Tonkabon ; Streptococcusis ; Risk factors ; Cold water fish ; Survey ; Rainbow trout ; Dissolved oxygen ; pH ; Nitrite ; Clinical signs ; Temperature ; Pathogen
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  • 72
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25620 | 18721 | 2018-10-14 02:36:27 | 25620 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The aim of this study was to find the best location for establishment of rainbow trout culture and hatcheries farms on Zayanderood’s river region in Charmahal and Bakhtiyari province. This survey carried out over ten station along Zayanderood’s river. The result of physical and chemical analysis showd that the annual average of air temperature varied from 9.5oC - 10oC where the pH annual average value were between 7.5 and 8.8. the dissolve oxygen concentration in stations except in rainbow trout farm effluent were above 10 mg/l. the other chemical , pollutant as well as pesticides levels were under the limiting concentration for rainbow trout culture and Hatcheries activity. The plankton survey showed that the Bacillurophyta were the dominant group of phytoplankton where protozoa constituted the most abundant group of zooplankton the Benthic organisms sensitive to pollutant in particular Epirus were dominant group in all stations. In regard to fishes presence in river, five species of Ciprinidae, one species from Balitoridae and one species from salmonidae families were identified. The capacity for development of rainbow trout culture for tow phase period in Zayanderood’s river region with respect to self purification potential (self purification potential were determined from the oxidation of the effluent of the only active trout farm of the river region), minimum of 10 L/s water requirement for production of trout in concrete canal and pond system and 1 L/S water need for production in semi circular closed system were estimated to be 5202 metric tons.
    Keywords: Aquaculture ; Iran ; Caharmahal and Bakhtiyari province ; Positioning ; Rainbow trout ; Self purification ; Culture ; Hatchery ; Survey ; Temperature ; pH ; Dissolved oxygen ; Pollutant ; Plankton ; Bacillurophyta ; Phytoplankton ; Protozoa ; Zooplankton ; Benthic organisms ; Ciprinidae ; Balitoridae ; Salmonidae
    Repository Name: AquaDocs
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  • 73
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25636 | 18721 | 2018-10-07 17:03:45 | 25636 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Tish survey prolonged from summer 2011 trough summer 2012. During this period 574 samples of different fish species were investigated for their parasites. Totally 30 species of parasites isolated of the fishes. Some of the isolated parasites are recorded for the first time in Iran. Rafidascaris acus Orientocreadium siluri, Silurotaenia siluri, Acanthocephalus lucii , Argulus foliaceus has recorded for the first time from European catfish, Silurus glanis, and Dactylogyrus inexpectatus has recorded for the first time from Gibel Carp, Carassius auratus gibelio, in Anzali wetland. Statistical comparison of parasites infections and intensity between the different area of Anzali wetland were done. Infection of Pike, Esox lucius, to Diplostomum spathaceum, Raphidascaris acus and Monogenean in western parts of wetland were significantly different from the Eastern and Central areas (p〈0.05). So based on the data have concluded the eastern and central regions of the wetland are more polluted than the western part. Comparing the results of the present study with before ones showed that the composition of parasite species has been changed over time, while the prevalence, intensity and abundance of parasites have been increased. It may be due to changing environmental conditions such as increasing discharge of effluent, eutrophication of the wetland. This results accents to necessity of reconstruction of Anzali wetland with preference of eastern and central regions.
    Keywords: Health ; Iran ; Anzali Wetland ; Anzali Lagoon ; Parasites ; Fish ; Infection ; Intensity ; Survey ; Samples ; Species ; Rafidascarisacus ; Orientocreadium siluri ; Silurotaenia siluri ; Acanthocephalus lucii ; Argulus foliaceus ; Catfish ; Gibel carp
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  • 74
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25646 | 18721 | 2018-10-08 08:29:31 | 25646 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Attention to the aquaculture capacity in inland water is needed to reach production of 262072 tons which was implicited in sixth economical and social development program of culture and production warmwater fishes industry. On of the most important aim of progressive management in fisheries program is native aquaculture activity with emphasis on Barbus fishes in Iran.Benni fish is one of the species of this genus despit of low relative growth rate, but is as a desire fishes in market. This fish not only has high fecondity and feed phytoplankton during the larval stages but also is very delicious for consumers. During the fifth economical and social development program there were so many projects have been done on aquaculture of this species but needs to analysis of all data which is important for successive future its aquaculture commercially. The precent analytical investigation base on three decades data, was done for completeing that gap including of analytical activities of researches were done, developmental access to fish aqu culture in six proficiency subjects , propagation and cultue, nutrition, genetic, health and disease biology, physiologt and determination of weak points. Then base on guideline studies on warmwater aquaculture and developmental road map, several suggestion for doing apply research were adopted.
    Keywords: Aquaculture ; Management ; Iran ; Aquaculture ; Banni fish ; Analytical projects ; Mesopotamichthys sharpeyi ; Investivigation ; Survey
    Repository Name: AquaDocs
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  • 75
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25648 | 18721 | 2018-10-08 07:22:08 | 25648 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Thirty to 40% of total fish catch is converted to waste. Using different methods of hydrolysis of the protein can be recovered of fish waste and increase the amount of protein efficiency. In this study, the four enzymes Alcalase, protamex, pepsin and trypsin were used for hydrolysis of four fish species including common carp (Cyprinus carpio), silver carp (Hypophthalmichthys molitrix), grass carp (Ctenopharyngodon idella) and the Big head (Hypophthalmichthys nobilis). The effects of pH, temperature and hydrolysis time on the rate of hydrolysis were studied on soluble proteins and degree of hydrolysis (phase I). In the second step, proximate factors of peptone been evaluated and eventually replace commercial peptone media MRS (Lactobacillus plantarum, Lactobacillus delberuki, Lactobacillus casei, Streptococcus thermophilus) and TSB (Listeria monocytogenes, two species of Bacillus and Pseudomonas, Streptococcus faecium) and the optical density of bacteria at different times were compared with control samples. Results showed that the highest degree of hydrolysis and soluble proteins were referred to alcalase and protamex, pepsin and trypsin respectively. The highest value of hydrolysis, in all treatments, was attributed to grass carp and silver carp, common carp and big head respectively. The best pH and temperature for alcalase, protamex, pepsin and trypsin 8.5and 55, 7.5 and 55, 3.5, 37, 7 and 37 respectively. Best time to achieve the highest degree of hydrolysis and soluble protein was 90 minutes. Qualitative analysis showed that the highest and lowest amounts of protein and fat in the treatment of alcalase (about 70 % protein and less than 0.5 % fat) and protamex, pepsin and trypsin was then. The results of bacteria culture showed that the highest percentage growth of lactic acid bacteria was referred to Streptococcus thermophilus and Lactobacillus casei had the lowest rate of growth. In other bacteria, Pseudomonas and Bacillus species were the highest percentage of growth and Listeria monocytogenes and Streptococcus faecium respectively. In all treatments, alcalase had the best results and the peptone prepared from fish waste grass carp had the best condition for growth of used bacteria. It seems that the initial substrate , the parameters used such as temperature, pH, and enzyme hydrolysis time , have a significant effect on the quality of peptone and protein content in the final product is determined value of protein for culture of bacteria.
    Keywords: Biology ; Iran ; Warm water ; Protease enzymes ; Bacteria ; Degree of hydrolysis ; Soluble proteins ; Fish waste ; Peptone ; Marine ; Culture ; Alcalase ; Protamex ; Pepsin ; Common carp ; Cyprinus carpio ; Silver Carp ; Hypophthalmichthys molitrix ; Grass carp ; Ctenopharyngodon idella ; Big head ; Hypophthalmichthys nobilis
    Repository Name: AquaDocs
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25654 | 18721 | 2018-10-08 08:21:14 | 25654 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The investigation of the algal bloom effects on Macro benthos abundance and diversity was seasonally carried out in coastline waters of Hormozgan province in 1391. Fourteen stations were selected for sampling; as 8 stations in coast of Bandar Abbas, 3 in Jask Port (east of Hormozgan) and 3 stations in Lengeh Port (west of Hormozgan). Polychaets, oligochaets, mollusks, crustacean and others like nematodes, nemertean’s, foraminifera’s, and ophiuroides were identified. Crustacean with 333950 ind.^-2 were dominant group whereas nemertean with 5050 ind.^-2 were minimum in density. Results showed that mean abundance of macro benthos was at most in spring (5614±3992 end) ^-2 and with 1244±380 ind.-2 was at least in autumn. Related results to stations showed that mean abundance in Jask Port and Lengeh Port were more than Bandar Abbas City. Among 8 stations in Bandar Abbas, Langargah with 168±977 ind.^ -2 was at most and Posht-e- shahr was at least in abundance (82±256 ind. ^-2). In Lengeh and Jask ports the stations far from coasts showed more abundance than station near coast. Monthly investigation showed that Macro benthos community was much more in April with 1011±6783 ind.-2 than December with 6±28 ind.^-2 , respectively. Shannon -Winner diversity index was low (0.61.2) in three stations, Evenness ranged from 0.1 to 0.4 and Margalof ranged from 2 to 4. Among four stations, mean wet weight varied from 12.18±6/95 gr in Jask Port (maximum) to 6.24±3/85gr in Bandar Abbas (minimum) .Seasonally, in spring the wet weight of macro benthos was 15.15±16/32 (maximum) and with 1.43±0.88 gr showed the minimum value in summer. There was a negative correlation between silt and macro benthos abundance and a positive correlation existed between macro benthos community and sand. There was no significant correlation between macro benthos community and TOM. To pay attention to results like diversity, abundance, wet biomass and correlation; it seems that algal bloom has no obvious negative effect on Macro benthos communities.
    Keywords: Ecology ; Iran ; Hormozgan Province ; Bandar Abbas ; Lengeh ; Jask ; Algal bloom ; Macro benthos ; Diversity ; Abundance ; Survey ; Polychaets ; Oligochaets ; Mollusks ; Crustaceane ; Biomass
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25652 | 18721 | 2018-10-08 08:43:54 | 25652 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: For the first time white spot disease (WSD) was reported in shrimp farms of khoozestan province, in southwest of IRAN in 2002. Then in 2005 the neighbor province, boushehr, was contaminated. In 2008 WSD outbreak reported in sistan-bloochestan province in southeast of Iran. In 2015 all of southern shrimp farms of country except Hormozgan, the middle southern province, which has remained free of WSD, are being contaminated. White Spot disease suspended shrimp culture in thousands hectares of shrimp farms. Considering that white spot disease has not been observed in Hormozgan province yet, the question is; to what extent environmental and management factors participated in preventing WSD outbreak or cause WSD outbreak. In this study (20102012), the effects of environmental factors and management, stressors that decrease immune system function of shrimp are discussed. In addition, the role of pathogen as the main factor of outbreak is discussed. The goal of this study is to define environmental parameters and management practices associates with outbreak of white spot disease in affected provinces and discover reasons of being Hormozgan province free of this disease. In this study the role of the local environmental factors and management practice stressors in susceptibility to WSD was determine. Both the effects of environmental factors in water of ponds including total ammonia, nitrogen, dissolved oxygen, pH, salinity, transparency, and temperature and management issues related to biosecurity are studied. There were overlaps on physical and chemical parameter values obtained in clear areas with contaminated areas .Results of the data analysis suggest that lack of association with WSD incidence was 7 times greater than WSD incidence despite of disease outbreak in sistan-bloochestan province, so other sources of white spot disease virus incidence was suspected in affected areas. Histopathological examinations and polymerase chain reaction (PCR) tests during project performance did not reveal white spot disease virus evidences in post larvae examined from khoozestan province stocked in farms but disease outbreak was happened in that farms , so we suspected to management practice include feed , pond preparation and carrier of disease. Recorded values of temperature and salinity in some months during inspection in Hormozgan province specified stressful condition that may lead to WSD outbreak, however the disease did not appear. Therefore the hypothesis that the water physical and chemical conditions are reasons to prevent disease outbreak in Hormozgan province is being rejected. The policy of Hormozgan’s fishery authorities, to replaced Fenneropenaeus indicus with specific pathogen free Litopenaeus vannamei, that is more resistant to some of diseases, before incidence of WSD in farms and to before being endemic in the Hormozgan province, made an advantage compare to affected southern provinces that introduced Litopenaeus vannamei after WSD prevalence to their farms. However it does not guarantee to maintain current trend of being Hormozgan province farms free of white spot disease. Therefore establishing the principals of biosecurity are strongly emphasized. Strategies taken by the proficient authorities in preparation of SPF shrimp broodstock can be the most important factor in preventing WSD. Regarding biosecurity principals purchased feed must be free of shrimp head powder. Construction the new shrimp farms should be as far as it could be away from contaminated areas.
    Keywords: Aquaculture ; Ecology ; Health ; Iran ; Khouzestan Province ; Boushehr province ; Sistan and Balochestan province ; Hormozgan Province ; Epidemiological study ; WSD ; Fenneropenaeus indicus ; Penaeus vannamei ; Shrimp ; White Spot Disease ; Temperature ; Salinity ; PCR ; Polymerase Chain Reaction ; Ammonia ; Nitrogen ; Dissolved oxygen ; pH ; Transparency
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25657 | 18721 | 2018-10-08 08:23:30 | 25657 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was conducted to spatio-temporal fluctuation and distribution of phosphorous and nitrogen species, total organic carbone and some environmental parameters at suface sediments in the southern of Caspian Sea. In current study, 48 samples were collected from four stations (Bandar Anzali, Tonekabon, Bandar Nowshahr and Bandar Amirabad) and three dephths (5, 10 and 20 meter) during four seasons in 2013-2014. Results of this study showed that annual mean of Adsorbed-P, Fe-P, Al-P, Bioava.-P, Ca-P, TIP, Rese.-P and TP were obtained 6.68±0.63, 60.90±2.44, 24.94±2.39, 94.52±3.62, 1868±36, 1963±37, 87.51±9.52 and 2050±38 µg/g.dw, respectively. Result also showed that inorganic phophotous was maximum during different seasons, however, percent mean of residue-P which containe organic compounds and non-degrediable compounds, was less that 5 percent. Percent of Ca-P was higher than 90, whereas Bioava.-P was less that 10 percent. The order of different forms of phosphorous were registered Ca-P〉Org-P〉Fe-P〉Al-P〉Adsorbed-P.Annual mean of NH4/N, NO3/N, TIN, TON and TN were observed 2.56±0.27, 0.89±0.12, 3.52±0.26, 6.06±0.27 and 9.57±0.32µg/g.dw respectively. Annual percent of TON was two folds than TIN and NH4/N was also three times than NO3/N. In addition, mean of Fe, Al, Ca, Eh and TOC% were obtained 25.39±0.65, 18.76±0.68, 60.15±1.50 mg/g.dw and 67.02±0.68, 1.11±0.09, respectively. Maximum mean of carbon (1152±171), nitrogen (0.87±0.05) and phoporous (74±23) µM/g were observed in fall, winter and summer, recpectively. Also, maximum ratios of TOC:TP (16.8±2.3) and TOC:TN (2378±363) were obtained in fall season. As a conclusion, mian role of Adsorbed-P, Fe-P and Al-P adsorbed and desorbed were temperature, Eh and pH, respectively. Results of this study showed that TOC:TN was very high which represented to excitance of organic matter with almost non-degrediable. Whereas, TOC:TP was low which showed that organic phophoros was not related to proteins and phospholipids therefore sources of org-P were belong to other compounds.
    Keywords: Ecology ; Iran ; Caspian Sea ; Anzali ; Tonekabon ; Nowshahr ; Phophorous ; Carbon ; Nitrogen ; Environmental parameters ; Surface sediments ; Nutrients ; Algal bloom ; Species ; Samples ; Temperature ; pH ; Proteins ; Phospholipid
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25670 | 18721 | 2018-10-08 08:56:10 | 25670 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The lack of sustainable management programs has resulted in the drastic decline of sturgeons stocks in the Caspian Sea in the past two decades. Legal catch quotas for all Caspian littoral states has dropped from 28500 tons in 1985 to 460 tons in 2007 while caviar production in the Caspian Sea during the same period decreased from 3000 tons to 70 tons. Caviar production in the I.R. of Iran dropped from 305 tons in 1985 to about 11 tons in 2007. The "Strategic and applied research planning for sturgeon management and conservation" was developed with the collaboration of academicians from universities, researchers, experts, fishery authorities and representatives of the executive government to maintain sustainable development and rational management of sturgeon stocks in the Caspian Sea. The preparation and development of this planning was carried out through 121 sessions (2100 person hours) A comprehensive report (375 pg) was prepared which was divided into five volumes; Volume I comprises an introduction, objectives, strategies and planning, present status of production and caviar harvest, catch and export quotas, revenue generated and release of sturgeon fingerlings, Volume II comprises analysis of the problem by constructing a problem tree with 8 broad categories to analyze 344 problems and an objective tree which is the hierarchic flowchart of objectives with 9 broad categories with 241 items, Volume III comprises a review and analysis of previous and ongoing research (414 projects) on different aspects of sturgeon during the past 40 years, Volume IV comprises prioritizing research objectives outlined by the objective tree and finally Volume V which prioritizes primary objectives for 14 executive and 10 research programs. On the basis of the evaluation of the present status and for the sustainable development and rational management of sturgeon stocks a strategic and applied research planning program was proposed and developed within the framework of three primary objectives; 1) Management and sustainable use of Caspian Sea resources (5 programs), 2) Rehabilitation and restoration of stocks (5 programs), 3) Development of aquaculture (4 programs). The applied research program for each proposed executive program was outlined in 10 programs, 42 comprehensive plans and 222 projects. The primary objective on management and sustainable use of Caspian Sea resources includes 5 programs, 14 comprehensive plans and 63 projects, while the primary objective on rehabilitation and restoration of stocks includes 4 programs, 16 comprehensive plans and 87 projects. The primary objective to address sturgeon aquaculture includes 1 program, 12 comprehensive plans and 72 projects. The impacts and outcome of each of these programs was determined and presented. It is evident that if the present situation persists, the catch figures for adult sturgeon specimens and caviar production in Iran will reach zero in 2021. But if concerted efforts are taken and the proposed strategic and applied research planning program is executed (commencing from 2009) we can put a halt to these declining trends. By producing sturgeon fingerlings to restore population abundances, by conserving and protecting them in the Caspian Sea and by conducting applied research we can produce 206.4 tons of caviar by the year 2033. Apart from harvesting caviar from the Caspian Sea we can also annually produce 3000 tons sturgeon meat and 60 tons farmed caviar starting from the year 2023. The total budget proposed for implementation of this program for a period of 14 years (2008-2022) is USD 2483 million. If this budget is allocated in time and the proposed strategic program is properly and totally implemented, we can not only save many sturgeon populations from extinction but also ensure job opportunities for 6000 fisherman, provide 1480 new job opportunities and annually produce 266 tons of caviar which will generate USD 4957 million.
    Keywords: Ecology ; Iran ; Caspian Sea ; Guilan Province ; Mazandaran Province ; Golestan Province ; Sturgeons ; Caviar ; Strategic ; Fingerlings ; Survey ; Fisheries
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25080 | 18721 | 2018-08-22 06:46:58 | 25080 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This project was conducted to survey production per hectare and other growth index of fenneropenaeus merguinsis and F. indicus in zar abzy fram in earthen ponds of Tiab area Hormozgan province. In this research for obtain aims used with two treatment and 3 replication. Postlarvaes were stocked at density 20 ind/m^2. Shrimp were fed by imported pellet on the basis of standard feeding table. In order to obtain the growth rate and calculation of daily feeding shrimp become biometry twice a month. Some of the physico chemical factor such and pH, dissolved oxygen and salinity were measured twice a month too. The results showed that mean weight of fenneropenaeus merguiensis were about 16.76 gr with mean daily growth 0.013 gr/day and for F. indicus obtain about 11.44 gr with mean daily growth 0.09 gr / day too. The results showed that mean F.C.R and survival rate in treatment 1 and 2 obtain 4.1 and 10 percent 1.7 and 64 percent respectively. The results showed that propagation of F.merguiensis is feasible and it can a culture species if suitable food is available.
    Keywords: Aquaculture ; Biology ; Iran ; Hormozgan province ; South Tiab area ; Final production ; Fenneropenaeus indicus ; F. merguiensis ; Survey ; Area
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25078 | 18721 | 2018-08-19 16:08:10 | 25078 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In order to create a reliable ecological database for Guilans running waters(north Iran near the Caspian sea), at the first step 3 important rivers containing Hevigh, Karkanrud and Shafarud, were selected and limnological survey, during autumn 1380 and summer1381 were seasonally carried out for them. Results showed that the key physico-chemical parameters such as dissolved O2, BOD5, pH, Phosphate, Nitrate, Nitrite, ammonium and in all of the three rivers water were in their normal range suggesting good water quality. Chrysophyta, Chlorophyta, Cyanophyta and Euglenophyta were respectively the most prevalent phytoplankton taxa and Zooplankton fauna of the rivers were fixed and sessile species belong to Protozoa and Rotatoria. 42 benthic macroinvertebrates taxa(Hevigh:36,Karkanrud:22 and Shafarud:30) were identified in these 3 rivers which individuals belong to order Diptera compromise the most divers and frequent one. Regarding macroinvertebrates diversity and indicator groups, it seems that Hevigh river has relatively better water quality than the two other. 23 fish species belong to 9 family,7 order and 2 class , were identified in these rivers during the study period which cyprinid were the most diverse and prevalent of them. Albornoides sp., Capoeta capoeta and Neogobios sp. were the most widespread and frequent species of the identified fishes in all of them. Bacteriological survey including Coliform and E.Colie count showed that the lower parts of the rivers near the estuaries were more infected and the bottom sediments had more bacterial count during the all sampling period. However the total coliform count never exceed of 200 colonies per 100cc , suggesting no risk for direct contact (swimming and washing), according to EPA standards. Chromium, Cadmium and Mercury had very low concentrations in the rivers water but Cupper had relatively high concentration (up to 1.788 mg/l in Hevigh river) amongst the studied heavy metals. The maximum and minimum concentrations of detergent materials or surfactant (LAS) were respectively observed in Shafarud (0.047 mg/l) and Hevigh (0.014 mg/l). According to the results of the study even though all the three rivers water had an overall reasonable quality, but some signs of destructions and degradations such as sedimentation, relatively increase of nutrient, increased concentrations of some pollutants, which all results in the low diversity of macroinvertebrates and prevents migration of anadromous fishes, calls for a continuing monitoring program and precise control for these regions.
    Keywords: Ecology ; Limnology ; Pollution ; Iran ; Caspian Sea ; S Caspian Sea ; Guilan province ; Karkanrud ; Shafarud ; Chrysophyta ; Water quality ; Phosphate ; Physico-chemical parameters ; Limnological ; Survey
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25263 | 18721 | 2018-09-07 07:55:35 | 25263 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: For hand feeding of Grass carp (Ctenopharyngodon idella), a kind of fish used in integrated carp culture, two kinds of pellets with Alfa alfa as current food were suggested as follows: 1. Pellet B with low fiber and high protein 2. Pellet C with high fiber and low protein The ingredient ratio was powdered and mixed, by adding water, fiber was made and minced the minced pellet was put on net tray and then in blotter to be dried and ready for use. In this experiment food quality analysis and also food water stability was measured. Regarding sampling, growth rate and weight increase of fish and FCR of each ratio was determined. Results indicates diet C is not able to compete with Alfa alfa from the stand point of weight increase, FCR and price. But diet B with low FCR and higher growth rate and higher price (not meaning ful P 5%) can compete with Alfa alfa.
    Keywords: Aquaculture ; Iran ; Grass carp ; Feeding ; Pellet ; Ctenopharygodon idella ; Fish ; Culture ; Alfa alfa ; Fiber ; Protein ; Sampling ; Growth ; FCR
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25265 | 18721 | 2018-09-07 07:24:14 | 25265 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Survey on physicochemical parameters and pollutants in artificial reefs in Hormozgan province (Bandar-e-Lengeh area) was conducted during 2004-2007. After establishing of artificial reefs by Hormozgan Shilat office, selection of sampling stations is performed at summer and winter seasons. Summer and winter values for temperature were 32.9 0.5 and 21.4 0.5 centigrade. pH had a little range of tolerance (8-8.95) and its annual mean was 8.81 0.13 . Transparency is an effective parameter on monitoring of artificial reefs. Summer and winter values for Transparency were 3.8 3.72 and 5.63 0.78 meters. Annual mean of dissolved oxygen and salinity were 7.11 0.1 ppm and 39.13 0.26 ppt. Annual means of dissolved nitrate, nitrite and orthophosphate were 4.9 0.7, 1.44 0.09 and 0.97 0.06 micromole per liters respectively. Measurement of chl.a was done in summer only. The maximum concentration was 2.95 microgram per liter. Annual average percentage of silt, clay and sand were 33 10.68, 21.63 4.84 & 45.96 12.66 respectively. Organic Carbon percentage had a little rang (0.61 1.92) of changes. Its annual percentage was 0.99 0.2. Summer and winter values for COD were 1.53 0.48 & 1.62 0.48 milligrams per liter. Concentration of heavy metals (Cu, Zn, Fe, Ni & Pb) in sediments were determined .All of heavy metals (especially Pb) had higher concentration in summer than winter. The annual means of Cu, Zn, Fe, and Ni & Pb were 26.5, 54.3, 27.6, 70.85 & 48.15 micrograms per gram of dried sediment. Normal aliphatic hydrocarbons (C10-C 30) were determined in water & sediment. Annual average and the summer and winter values for normal aliphatic hydrocarbons in water were 258.7, 217.8 & 299.6 micrograms per liter and for sediment were 5726.8, 12616.8 & 2132.2 micrograms per gram of dried sediment.
    Keywords: Ecology ; Pollution ; Iran ; Persian Gulf ; Hormozgan Province ; Bandar-e-Lengeh ; Artificial reef ; Heavy metals ; Aliphatic normal hydrocarbons ; Physicochemical parameters ; Survey ; Pollutants ; Sampling ; Temperature ; pH ; Transparency ; Nitrate ; Nitrite ; Sediments
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25299 | 18721 | 2018-09-07 08:25:10 | 25299 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Considering increase in production of earth ponds in brackish water condition, this research carried out at Bafgh fisheries research station to improve efficiency and production of brackish water cultural earth ponds of rainbow trout (Oncorhynchus mykiss). This study was performed in four 0.5ha earth ponds in two treatments with two replications. Each treatment included two earth ponds so that fish farmed in two culture mediums consisting open medium and %20 enclosure medium of pond (pen medium).for this purpose 20% of earth pond s space separated via a fixed net wall with 15mm in mesh. In this research some main growth equations and economical factors were studied. Data were analyzed using SPSS software, Pierson, Toki, Dankan, Dant analysis and also descriptive statistics. The significant differences were determined in %5 level. Since there was a connection between pen medium with free one, no signs based upon disturbance observed in measured environmental factors in this space. Statistical results indicated a significant increase in %WG, %FE, % SGR, %CF and final biomass in pen medium treatment(p〉0.05). According to economic results, this treatment also differed significantly from open medium treatment about ratio of marketable size production and net income so that yield quantity and production income increased. Therefore, through pen culture in 0.5 ha earth ponds and likely larger ones, utility and production of farm improve and can be a good approach for farmers in order to better exploitation of possibilities and facilities.
    Keywords: Aquaculture ; Iran ; Bafgh ; Surrounding medium ; Earth pond ; Rainbow trout ; Utility ; Brackish water ; Culture ; Oncorhynchus mykiss ; Growth ; Yield
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25297 | 18721 | 2018-09-07 08:04:10 | 25297 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Mazandaran and Gorgan provinces have temperate climate, thus they have more potential for aquatic animal culture. There are thousands hectare of salt and useless lands in adjacent to Caspian Sea. As these areas have provided a favorable back ground for aquatic animal culture. As a result, the successful results obtained from imported gray mullet (Mugil cephalus) culture project in north climate, it has demonstrated that the gray mullet has a good biocharacteristic for culturing in pond enclosure environment and in different aquatic conditions (fresh water, brackish water and salt water). From 1998 until 2001, the broodstock yield and gray mullet artificial propagation projects were performed by fisheries research center of Mazandaran in Ghomishan prawn culture station in adjacent to Caspian Sea (East north of Mazandaran Province). This investigation executed during two stages (phases). At first stage, the goal of this project included the survey of possibility available about matured fish as well as induction of final maturation and artificial propagation for producing of broodstock and larva. In addition, in this way, we will obtain new information about gray mullet propagation and culture as marine species. We introduce mass production in aquaculture. At present research, the possibility of broodstock yield and artificial propagation of gray mullet have investigated by gray mullet fingerlings imported from Hong kong and then they have cultured in earth ponds of Ghomishan areas during five years (1994-1998). In order to broodstock production in spring 1998, two earth ponds (0.5 hectare) were prepared. 100 specimens of fish stock (1-2.5 kg weight average and 5 years of age) placed in each pond. For providing of suitable water and nutrition, fish were fed by food containing rich protein (40%) with 3-5% body weight and maintained in water with 30-35 ppt salinity. The survey of sexual maturation was performed by sampling of sexual glands through year. There were four stages in dominant female broodstocks. This survey indicated that oocytes have emerged stage 1 (immature) from March to June, stage 2 (yolk vesicle) in September and stage 4 (yolk globule) in October. Three stages (first, second and third) of yolk formation in oocytes will occur but these stages take place in October, December and January respectively. It's obvious that oocytes will progress into the end of third stage (yolk formation) and then their growth was arrested. Ovum with 600 m diameter was observed when the water temperature declined less than 18 c and day time was short (from middle autumn to middle winter), on that time, fish were induced by hormone because lack of final maturation and ovulation, therefore, natural spawning was not occurred in pond condition. Furthermore, artificial propagation of mugil cephalus was occurred by hormonal induction. Hormonal induction was utilized by inject of many hormones (LHRH, Cph, HCG). Using different components of hormones and also their different doses obtained the best results from broodstock that the average ovum diameter was about 600 m. Several types hormones which were injected into gray mullet with two or several intermittent (24 h intervals) along with 20-25 c temperature and 30-35 ppt salinity. This condition can provide stage 4 maturity for fertilization. Totally (as whole), three intermittent fertilization was necessary for exiting of larva, larva production in first, second and third intermittents that were 2000, 2500 and 300 specimens respectively. Larva fed on chlorella algae and rotifera and they have maintained for 14-15 days. The results of obtainable research indicated the possibility of gray mullet broodstock production in cultural condition, artificial propagation and larva production. This research took place for the first time in north climate of Iran.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Mazandaran Province ; Gorgan ; Ghomishan ; Gray mullet ; Mugil cephalus ; Broodstock ; Production ; Yield ; Artificial propagation ; Hormone ; Temperature ; Culture ; Maturation ; Fingerlings ; Earthen ponds ; Ovum ; Spawning ; Breeding ; Reproduction
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25298 | 18721 | 2018-09-07 08:30:09 | 25298 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The marine survey for sturgeon stock assessment was conducted in summer, winter and spring in the years 2006 and 2009 to estimate the relative and absolute abundance and percentage composition of each species in the Guilan, Mazandaran and Golestan Provinces. This survey was carried out in the Iranian waters of the Caspian Sea on board the Sisara2 and Guilan vessels using trawl nets at 2-100 m depths. Trawling was carried out in 85 stations that were selected using a stratified random design. The number of stations in each scope was based on the area of the scope in terms of the total area. Trawling and sampling in shallow water up to 10 m were carried out using 9 m trawl nets whereas 24.7 m trawl nets were used for depths more than 10 m. Trawl surveys were carried out in the daytime. Trawling velocity was kept at 2.5-3 kts and trawls lasted half an hour in order to calculate abundance, and biomass of sturgeons using the swept area method. Catch per unit area (CPUA) in the winter 2006 survey was 3853 specimens nm^2 , in the summer and winter 2007 survey was 1854 , 2912 specimens nm^2 at depths less than 10 m respectively . CPUA for sturgeons in spring 2008 survey was 2103 specimens nm2 at depths less than 10 m and 393 specimens nm2 at depths greater than 10 m (10-100 m depth). These values in the winter 2008 survey dropped to 44 specimens nm^2 at depths at depths above 10 m. CPUA for sturgeons in the spring 2009 survey was 300 specimens nm^2 at depths less than 10 m and 307 specimens nm^2 at depths greater than 10 m. In all the surveys conducted CPUA for A. persicus was higher than that for the other sturgeon species. Based on the calculations carried out in the marine survey in winter 2006 the estimated absolute abundance for sturgeons was about 2977.363 thousand. The total biomass of sturgeon was estimated as 131.713 tons. In the summer 2007 survey total abundance was estimated 1432.398 thousand, and total biomass of sturgeons was estimated at about 312.161 tons. In the winter 2007 survey total abundance for sturgeons was estimated at about 2250.105 thousand, and total biomass was estimated 578.08 tons. In the spring 2008 survey total abundance was estimated at about 3002.832 thousand. The total biomass was estimated at about 2533.318 tons .In the winter 2008 survey total abundance was estimated at about 152.722 thousand, and total biomass in winter 2008 was estimated 170.540 tons. Total abundance in spring 2009 survey was 1310.232 thousand and total biomass was estimated at 2019.tons. Investigation of stomach content of sturgeon Acipenser persicus caught under 10m depth in 2006 to 2007 surveys showed that there is significant difference in the consumed food. Polychaeta is the major food consumed and crustacean an the minor one(P〉0.05).Also no new types of food( such as bony fishes or benthic) have been observed in food chain of Acipenser persicus only the food consumption rate has been related to the season of year and increases or decreases in warm or cold seasons, respectively For physiological study and determination of sexual maturation stages in sturgeon,119 gonad sampling prepared. The results showed that 63 % of fishes were females and 37 % of them were males. Gill microscopic study shows complications such as hyperplasia, curvature, adhesion, embowed, shorting and lengthen of secondary filaments and fraught bloody. Microscopic study on liver indicates signs of cloudy inflammation, fatty degeneration, dispersion of billed secretions and cell atrophy. The population genetic structure of Persian sturgeon (Acipenser persicus) in Sefidrood and Gorganrood rivers watershed analyzed based on microsatellite markers during sturgeons assessment in 2006-2008. Results showed that Acipenser persicus in two region of south part of Caspian Sea are two independent populations.
    Keywords: Fisheries ; Iran ; Caspian Sea ; Mazandaran Province ; Golestan Province ; Guilan Province ; Sefidrood River ; Gorganrood River ; Stock assessment ; Sturgeon ; Acipenser persicus ; Diet ; Gills ; Sex ; Microsatellite ; Population ; Genetic ; Survey ; Species ; Sampling ; Trawling ; CPUA ; A. persicus ; Biomass ; Polychaeta ; Maturation
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25305 | 18721 | 2018-09-07 08:31:01 | 25305 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This project was carried out in order to increasing of nutritional value, taste and shelf life of cleaned Kilka Fish during cooled storage. Edible films made by Whey protein and Sodium alginate were used for fish packaging. This search carried out in two stages consisting of pre- study and study. 3, 6, 9 and 12% concentrations of Wp and 0.5, 1, 1.5 and 2% concentrations of SA at three times including 0, 2 and 4 hours were used in pre-study stage. The covered samples were kept in -18 ֯C. Microbial and sensory examination were carried out for a period of two months. Microbial factors were including total bacterial count, Staphylococcus bacteria count, Coliform, Escherichia coli and Pseudomonas bacteria. Sensory tests consist of taste, odor, color and tissue were studied in the fish samples. 12% and 0.5 % concentrations at time = 0 of edible films made by WP and SA considered in study stage. This is can be due to the significant differences in total acceptance index of sensory tests. Control sample cleaned Kilka was packaged in disposable dishes with cellophane covers in 500gr in weight. Two selected timar and mixed cover including 12 % and 0.5 % concentrations at time = 0 of edible films by WP and SA considered in study stage. The covered samples were kept in -18 ֯C. Microbial, chemical and sensory examination were carried out for a period of six months. These factors and chemical factors consisting of humidity, protein, lipid, ash, calorie, Peroxide value, free fatty acids, thiobarbitoric acid, TVN and pH were studied in test samples compared with the control samples. Coliform, Escherichia coli and Pseudomonas bacteria contamination were negative until the end of storage period in the covered samples. The mean total bacterial count and Staphylococcus bacteria counts in processed samples by WP were 2.47 and 1.61 logcfu/g, in processed samples by SA were 2.84 and 1.28 logcfu/g, in processed samples by WPSA were 2.51 and 1.44 logcfu/g, and in control samples 4.11 and 2.93 logcfu/g from 1 day until six months after processing, respectively. The mean of moisture, peroxide value, TVN, pH, free fatty acids, thiobarbitouric acid , protein, fat, ash and calorie in the covered samples by WP were 73.91%, 0.13 meq/kgoil, 9.84mg/100g, 6.15, 1.15gr/100, 0/006 mg/kg, 19.00%, 4.25%, 2.1% and 120.73 kcal/kg, in the covered samples by SA were 73.91%, 0.06 meq/kgoil, 9.84mg/100g, 6.15, 1.15gr/100, 0/006 mg/kg, 18.85%, 4.72 %, 1.90 % and 125.98 kcal/kg, in the covered samples by WPSA were 73.91%, 0.06 meq/kgoil, 9.84mg/100g, 6.15, 1.15gr/100, 0/006 mg/kg, 18.50 %, 4. 65 %, 2.25 % and 126.48 kcal/kg and in control samples 59.43%, 3.25 meq/kgoil, 16.22mg/100gr, 6.71, 9.21gr/100, 0/15mg/kg, 18.2%, 4.00%, 1.80% and 107.10 kcal/kg, respectively. No statistically significant differences were observed in the results of chemical experiments of the covered samples and presence of the meaningful difference at the results of the chemical experiments of the control sample, The covered samples by WP, SA and WPSA up to the end of storage period at cold-room had a favorite quality but the control samples had lost their. No statistically significant differences were observed in the WP samples compared with the WPSA samples (p〉 0.05). Samples covered by SA had better quality compared with other samples which can be due to the presence of the significant difference in total acceptance index among covered samples without considering of economical worth.
    Keywords: Fisheries ; Iran ; Edible films ; Kilka packaging ; Quality evaluation ; Cooled storage keeping ; Chemical analysis ; Protein ; Sodium ; Samples ; Bacterial ; Protein ; Lipid ; Fatty acids ; Escherichia coli ; Pseudomonas bacteria ; pH
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25321 | 18721 | 2018-09-08 16:49:00 | 25321 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This research was performed to survey the live handling condition of Rainbow trout in Tehran province and determine the effects of this method on the quality of the flesh regarding with water binding capacity, pH changes, moisture, fat and protein content in different seasons of year. Based on reviews of Questionnaires, there were 20 main live rainbow trout sale centers in Tehran. 10 numbers of these were in fruit and vegetable centers of municipality and others were out of these centers. Live fish handling to in these areas mainly was performed from fish farms located in the Haraz road (85 percent), farms of Firoozkooh road (10 percent) and others were (5 percent) from neighboring provinces. The results of this study indicated that current methods of live handling in Tehran (the transporting time 4 h and the density of fish in tank 100-150 kg /m^2) meat quality of transported trout was not affected. Water holding capacity of transported fish was less than caught fish in the fields. This amount was reached to lowest level in in summer as well as the differences between the two groups were significant (P〈0.05). Also, when a meat pH level was elevated, water holding capacity was reduced. In summer, the amount of WHC in transported fish was 13.4% that was a highest value between the seasons. Appears that in the summer, high temperature and increasing kinds of stresses to fish during handling due to excessive accumulation of metabolites such as ammonia, muscle energy sources emptied because of high activity. In this condition, falling of pH during postmortem changes being moderated and lead to increase drip loss in the transported sample.
    Keywords: Aquaculture ; Fisheries ; Iran ; Tehran province ; Haraz ; Firoozkooh ; Live Handling ; Rainbow trout ; Meat quality ; Seasons ; pH ; Moisture ; Fat ; Protein ; Temperature ; Sample
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25118 | 18721 | 2018-08-22 02:17:23 | 25118 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Studies show that thyroxine can play an important role in regulating growth and other physiology activities. Since the direct role of thyroxine in growth metamorphose larval of bony fishes hasn't been proved yet, during the experiment accomplished in Shahid Ansari and Jajrood station, the hormones function in the survival of development of silver carp, grass carp and rainbow trout were studied. In this study accomplished of thyroxine baths with 0.1, 0.5 and 1 ppm (with 3 repeat). The other phase, thyroxine injected to females grass carp, silver carp, rainbow trout and barbell of Caspian Sea with different doses (1, 10 and 100 g/g B.W.). Tehn survival of development of embryo and larval and precent of fertilization were studied. Also, the percent of mortality were compared in two phases in stages of development. Results show that: 1) Trout: a) Phase of thyroxine bath: The number of hatching eggs and survival of larval in 0.5 ppm were increased to other treatment. b) Phase of hormone injection: The survival of larval in treatment of 10 g/g was 8.58% that was meaningful difference to other treatment (P〈0.05). 2) Silver carp: a) Phase of thyroxine bath: The number of hatching eggs and survival of larval in 0.5 ppm were (20%) increased to other treatment (P〈0.5). b) Phase of hormone injection: The survival of larval in treatment of 10 g/g was meaningful difference to other treatment (P〈0.05). 3) Grass carp: a) Phase of thyroxine bath: The number of hatching eggs and survival of larval in 0.5 ppm were 39% increased to other treatment. b) Phase of hormone injection: The survival of larval in treatment of 1 g/g was meaningful difference to other treatment (P〈0.05). 4) Barbel of Caspian Sea: Development stages wasn't determined in this fish. Number of eggs degenerated, number of eggs were the first development stages and growth of numbers increased on ovary.
    Keywords: Biology ; Chemistry ; Iran ; Caspian Sea ; Thyroxine ; Female ; Survival ; Growth ; Egg ; Larval ; Grass Carp ; Silver Carp ; Thyroxine (T4)
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25124 | 18721 | 2018-08-26 13:25:27 | 25124 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The brine shrimp Artemia consists of a number of bisexual species and a large number of parthenogenetic forms, which collectively, inhabiting a wide range of hypersaline habitats. This research was carried out in order to study the genetic differentiation of Artemia urmiana and two parthenogenetic populations originated from surrounding lagoons of the Lake Urmia (Fesendoz, Saray village) and also to find the phylogenetic relationships of some Iranian Artemia (Qom, Arak, Golestan, and Shiraz) with a comparison to some foreign Artemia species (A. sinica, A. franciscana, A. parthenogenetica (Russia and Turkmenistan) using a molecular technique (PCRRAPD`s). Of a total number of 56 random primers (10 bp) survey, 29 and 26 oligonucleotides showed polymorphic patterns with highly reproducible bands in Artemia urmiana groups and Iranian Artemia groups.The highest percentage of polymorphic loci were in Artemia urmiana (P= 78.89 %) and in comparison with the other parthenogenetic forms, in parthenogenetic Artemia from Saray village (P= 59.8 %). The highest values of Shannon index were estimated in Artemia urmiana (I= 0.41), and in comparison with other parthenogenetic forms, in parthenogenetic Artemia from Saray village (I= 0.3). Analysis of molecular variance indicated that most variation were within populations (A. urmiana with parthenogenetic forms, FST= 0.35) and among populations (Iranian Artemia with foreign species, FST= 0.76). Genetic pattern was not in accordance to geographical distribution, which indicated the wide range of Artemia samples at different regions in Iran. UPGMA showed two distinct clusters in group I including A. urmiana with two parthenogenetic forms and four clusters in group II including Iranian Artemia with some foreign species. It is understood that two parthenogenetic populations (Fesendoz and Saray village) had close genetic relationship together with high value of heterozygosity in parthenogenetic Artemia from Saray village. Iranian Artemia (Qom, Arak, and Golestan) indicated close genetic relationships with A. parthenogenetica (Russia and Turkmenistan) with low values of heterozygosity. A sister group was generated including Shiraz and USA Artemia samples which attests a possible contamination of local water with A. franciscana. FST value revealed high genetic structuring at all studied groups. Besides, it is suggested that gene flow was not strong enough to prevent substantial genetic differentiation. Result could lead to a management programmes to use suitable Artemia strains with high genetic diversity, for culturing and establishment of gene bank of Artemia stocks in Lake Urmia.
    Keywords: Biology ; Fisheries ; Iran ; Lake Urmia ; Fesendoz village ; Saray village ; Artemia urmiana ; Parthenogenetic populations ; Iranian Artemia ; Phylogenetic relationships ; RAPD`s ; Shrimp ; Bisexual species ; Phylogenetic ; A. sinica ; A. franciscana ; A. parthenogenetica ; Survey ; A. urmiana
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25337 | 18721 | 2018-09-13 12:43:04 | 25337 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was conducted to determine the physico-chemical characteristics of water during four season and 8 transects (Astara, Anzali, Sefidroud, Tonekabon, Nowhshar, Babolsar, Amirabad, Bandar Tourkman) in the Southern of Caspian Sea in 2009-2010. 480 samples were collected at different water layers and then physicochemical parameters were measured based on standard methods. Result of this study showed that surface water temperature was varied from 7.2 to 29.8◦C in winter and summer, respectively. Minimum fluctuation of water temperature was observed at 100 m depth (6.8-10.3◦C). Mean value of water transparency was obtained 4.91±0.24 m. This value increased from inshore to offshore. pH value was fluctuated from 7.15 to 8.83 unit with variance of 1.54. Maximum DO concentration was observed at surface layer (8.40±0.08 mg/l) with 137±18 saturation and minimum was at 100 m depth (6.46±0.18) with 86.1±2.8 saturation. The nitrite, nitrate and ammonium concentration were ranged 0.0-0.2, 0.0-4.6 and 0.05-7.12 µM, respectively. Maximum value of TN was observed at inshore and minimum at offshore (100m). Nitrite concentration decreased from inshore to offshore but increased from surface to the bottom (100 m). The inorganic phosphorous increased at surface water and also at the bottom. Trend of inorganic and TP was similar. Minimum of N/P ratio were observed at summer (5.48±0.38) and maximum value at winter (9.13±0.46). This value of N/P ratio showed that the growth of phytoplankton was limited by nitrogen. The dissolved silicate was decreased from spring (230.7±6.65 µg/l) to winter.
    Keywords: Ecology ; Iran ; Caspian Sea ; Astara ; Anzali ; Tonekabon ; Nowhshar ; Babolsar ; Bandar Tourkman ; Sefiroud River ; Water ; Physico-chemical parameters ; Temperature ; pH ; Phosphorous ; Phytoplankton ; Nitrogen ; Dissolved silicate ; Fluctuation
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25350 | 18721 | 2018-09-14 07:03:37 | 25350 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This survey was carried out in 8 lines of southern area of Caspian sea consisting of Astara, Anzali, Sefid roud, Tonekabon, Noshahr, Babolsar, Amirabad and Torkman in depths of 5, 10, 20, 50 and 100m in 4 seasons (spring, summer, autumn and winter) of the year 2008. The goal of this survey was identification of different species, distribution, density and Biomass of the zooplankton community in different regions and also their frequency in the different layers and depths and the population fluctuations in whole of year. The most abundance and biomass of the zooplanktons were 28005±24212 ind./m^3 and 180.58±149.03 mg/m^3 in 5 min spring and they were reduced gradually to depths. Copepoda comprised between 9 to 16 percent, Rotatoria comprised between 5 to 13 percent and Lamellibranchiata larvae comprised between 27 to 70 percent of marin zooplanktons. The most abundance and biomass were 11746±7921 ind./m^3 and 47.07±31.96 mg/m3, 5281±5521 ind./m^3 and 29.03±31.68 mg/m^3 in 5m of summer and autumn seasons, respectively. Copepoda comprised more than 95 percent of marine zooplanktons in all of depths in these seasons. The most abundance and biomass were 19030±16518 ind./m^3 and 198.99±217.23 mg/m3 in 5 m in winter. Copepoda comprised between 17 to 49 percent and Rotatoria comprised between 32 to 70 percent of marine zooplanktons in all of depths and the abundance of other groups was not considerable in zooplankton community.
    Keywords: Ecology ; Iran ; Caspian Sea ; Astara ; Anzali ; Sefidroud River ; Tonekabon ; Diversity ; Abundance ; Zooplankton ; Survey ; Density ; Biomass ; Population ; Distribution
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25354 | 18721 | 2018-09-13 13:02:58 | 25354 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: 500000 juvenile Indian majour carps (Labeo rohita,Cirhinus merigala, Catla catla) imported (2004) and transferred to the earthen ponds in Shahid Ansari Carp Breeding Center , In order to assess the viability of rearing under the climatic condition of north part of Iran and also achieve some growth parameters from the larval stage to market size.The fry with 300 mg weight, were released in to 8 ponds.specimens were separated to tree experimental categories: 1= (Labeo rohita) , 2= (Cirhinus merigala) , 3= (Catla catla). fishes were fed with artificial food and also with the natural products of the ponds which were enriched with organic fertilizers, after being equally stocked in the ponds .During the experiment, oxygen level ,PH, turbidity, and temperature were measured . Monthly fry samples were taken, for weight and length biometrical analysis and also determination of the weight gain percentage, average daily growth rate, specific growth rate. Results have indicated adaptations and considerable growth in this species, with an increase from the initial weight of 300 mg to an average of 13.5±1.6 mg in 50 days period until the end of November for fingerlings. Also the average weight of growing phase (second year of production) was 647g. For Labeo rohita ,420 g. for Cirhinus merigala and 734g. For Catla catla. The special growth rate(SGR)in Roho was 4.51 %.and 3.9 % for merigal and6.2 % for Catla while for Chinees carps SGR was 4.9, 6.7, 7.6 and 5.8 for silver carp,grass carp, common carp and big head respectively. Results revealed that the Indian majour carps compatibility with the condition in Gilan province was succesfull and the combination of species and optimization of their compatibility were remind for further studies.
    Keywords: Aquaculture ; Iran ; Indian carp ; Growth ; Labeo Rohita ; Cirhinus merigala ; Catla catla ; Juvenile ; Breeding ; Rearing ; Growth ; Larval stage ; Fry ; Artificial food ; Oxygen level ; pH ; Turbidity ; Temperature ; Species
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25126 | 18721 | 2018-08-26 13:44:22 | 25126 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this research in order to assess the possibility of antioxidant effects in quality protection and increase the shelf alife of fish burgers, ascorbic acid as a antioxidant by natural source used in raw uncoated fish burgers and in order to comparison by vacuum packaging, 3 treatments of uncoated fish burgers produced from cultivated silver carp: 1- burgers by common packaging (control) 2- burgers by vacunm packaging 3- burgers by 500ppm Ascorbic acid. Also in order to comparison BHA+BHT antioxidants (that have synergistic effects to each other) effect by vacuum packaging to prevention of lipid oxidation in semi-fried fish burgers 3 other treatments produced too: 1- burgers by common packaging (control) 2- burgers by vacunm packaging 3-burgers by 200 ppm BHA+BHT antioxidants comparatively to fats of product. All of the burgers after production and freezing conserved in -18°c for 6 months (Raw uncoated burgers) and one year (Semi-fried coated burgers). During the storage period chemical, microbiological, and organoleptic tests were down by three repetition monthly. Although peroxide value in raw uncoated fish burgers were higher than standard range even from first month but it seems this factor is not suitable for quality evaluation of uncoated raw fish burgers. Evaluation of TBA index in raw uncoated fish burgers during storage time showed at the end of storage period TBA index for control, vacuum and ascorbic acid treatments were 6.31, 4.76 and 1.29 mg malonaldehyde/kg respectively and taste scores were 5.11, 5.42 and 6.16 respectively. Results indicate the positive effects of ascorbic acid to prevention of lipid oxidation. By attention to TVN, TBA and organoleptic tests 4 mounths for treatments without ascorbic acid by vacuum packaging preference and 6 months shelf life for ascorbic acid treatment have suggested in -18°c temperature. For semi fried fish burger 28 prevention effect for lipid oxidation but vacuum packaging had 32 (without significantly difference,P〉0.05). so we can for %BHA+BHT treatment this property was recommend the use of vacuum packaging instead of antioxidant treatment. By attention to TVN, pH, PV, TBA and microbiological and organoleptic tests we can suggest the 8 months for shelf life time of semi fried fish burgers. In this product TVN was the only limitation factor and exeeded from standard range at 9th month of maintenance.
    Keywords: Fisheries ; Iran ; Survey ; Silver Carp ; Burger ; Ascorbic acid ; BHA ; BHT ; Lipid ; organoleptic temperature ; Temperature ; Vacuum packaging ; Common packaging ; Fish
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25128 | 18721 | 2018-08-26 13:26:24 | 25128 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Limnological studies of the Zarrineh River have been performed in order to identify physicochemical and biological characteristics of this river. Water samples from 14 sites along the river length were examined monthly during a year. The results indicated the river was polluted by Saghez and Miandoab Cities sewage to the extent of poly saprob, the extent of pollution at other sites was within the limits of oligo saprob or meso saprob. The results also showed that all dissolved and mineral compositions in this river was safe for drinking but iron concentration and water hardness in the terminal sites near the Urmia Lake are higher than EPA standards for drinking . This cannot, however, pose serious problem for warm water fish culture activities. Domestic sewage seem to be the most important pollution source to this river. Although the self-purification capacity of this river seem to be high but large number of polluting sites along the river exceed this capacity.
    Keywords: Limnology ; Iran ; Miandoab city ; Saghez city ; Zarrineh River ; Urmia Lake ; Limnological ; Pollution ; Mineral ; Fish ; Culture
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25130 | 18721 | 2018-08-26 13:37:55 | 25130 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This survey was designed in the Helleh and Delvar regions, to understand effects of aquaculture industry on coastal waters of Bushehr, in 2003. In Delvar region, five stations were selected comprise; effluent canals of Delvar 18 and 14, sea (south and north of site), influent water (Piyazi bay). In Helleh region, two stations; effluent canal and Ramleh bay were selected. Sediment and water samples were collected from effluent canal, influent canal and sea during the 9 month period (before until after culture season). The variations of selected water quality parameters were controlled monthly. Results show that, the maximum amount of ammonium in Helleh region was 0.33 mg/lit and in regions of Delvar 18 and 14 were 0.19, 0.20 mg/lit. However the maximum amounts of phosphate in Helleh region was 0.26 mg/lit and in regions of Delvar 18 and 14 were 0.24, 0.23 mg/lit. Correlation coefficients assess between phosphate concentration of effluent canal and sea station (gate of Ramleh) in Helleh region was r=0.91 (α=0.01). Also correlation coefficients
    Keywords: Aquaculture ; Iran ; Bushehr Province ; Helleh region ; Survey ; Aquaculture ; Sediment ; Samples ; Culture ; Coastal waters ; Shrimp ; Parameters ; Water quality ; Comparison
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25390 | 18721 | 2018-09-14 07:50:08 | 25390 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The present research aimed to study effect of Nereis worm in feeding, growth, survival and carcass biochimical compositions of persian sturgeon larvae. Five diets including zooplankton (100%) as the control (Z), Nereis diversicolor worm (100%), a mix of Nereis and zooplankton (50% for each), a mix of Nereis, zooplankton and concentrate food (33% for each) with 3 replicates were established. At first, Nereis worms were cultured up to the weight of 200 mg according to the protocol done in the International Sturgeon Research Institute. Required zooplanktons were obtained for Dr. Beheshti Sturgeon Rearing and propagation center. Concentrate food was also made in the ISRI. Persian sturgeon larvae with the average weight of 95.66 mg were put in 60-Liter tank (60 larvae in each tank) under the same physical and chemical parameters of water. 8 days after the beginning of active feeding, they were fed five times a day with experimental dites based on 20-30% of their weight for 15 days. Water quality parameters, such as dissolved oxygen, temperature, pH values were recorded daily. The average of temperature, pH and dissolved oxygen during the test were 22.8±1.3ºc, 7.5±0.1 and 6.58±0.9 mg/l respectively. At the end of this period, condition factor (k), specific growth rate (SGR), food conversion rate (FCR), weight gain (WG) and Body weight index (BWI) were calculated. Total fat and fatty acids profile were analyzed. The results showed that there was a significant difference between growth indexes and food consumption (p〈0.05). BWI, GR and SGR indices showed no significant difference between N and NZ treatment, but the average of these indices were higher in NZ treatment. The most and the least average of SGR, BWI were observed in NZ and NZC treatments respectively. Condition factor showed no significant difference in all diets except NZ. FCR had no significant difference between N and NZ as well as after diets. The highest and the lowest survival rate was observed in N treatment (96.11±1.46%) and NZC (85.55±3.37%) respectively. The results of carcass analysis showed that there is a strong correlation between fatty acids of body and fat resources of diets. According to the significant difference in growth rate between treatments (p〈0.05), the larvae fed with N diet presented a better performance compared with others. These results indicate that cultured sturgeon larvae need n-3 and n-6 series of fatty acids in their diet.
    Keywords: Aquaculture ; Iran ; Acipenser persicus ; Nereis diversicolor ; Growth ; Survival ; Fatty acids ; Larvae ; Feeding ; Persian sturgeon ; Zooplankton ; Dissolved oxygen ; Temperature ; pH ; SGR
    Repository Name: AquaDocs
    Type: monograph
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    Format: application/pdf
    Format: 69
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25380 | 18721 | 2018-09-14 07:58:08 | 25380 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Esfahan province is located in center of Iran at 51° 31 ' E and 33° 29 ' N with area near 15745 Km^2 that consists of 9 percent of the country. According to cilianinof scale it enjoys an arid climate. The mean annual precipitation is equal to 135 mm. Average annual temperature is 16.8°C and mean annual evaporation is 2435 mm in Gavkhoni catchment's area. It has several drainages channels such as north and south rodashtain, segzi drainage, main drainage of water supply organization channel, fender drainage, main drainage, Marchi class II drainage and other drainage channels. Their waterdebi volume is equal to 900l/s. Also, their water can not use for agriculture, drinking and industrial purposes due to high salinity. This study was concluded to optimal use of these water resources aiming at possibilities of culture and propagation of Artemia from Urmia Lake. In this study, physicochemical factors analysis of water, the climate profile, and fauna and flora of the region were identified and determined. In rodashtain region drainages, 2 sites in varzaneh and Sian drainages from gavkhoni wetland limitation were chosen and studied. Water samples in 50-100 liter volumes were taken from their run off waters with 3 replicates. Artemia cysts were hatched under standard laboratory condition according to Lavens and Sorgeloos, 1996). Cyst hatching was carried out in 1.5 liter conical zooks 2 g of Artemia cyst per one liter of drainage water was added to each treatment together with one evidence. Hatching percentage and hatching efficiency were determined. Hatching percentage and hatching efficiency after 76 hours were equal to 55±10 percent and 92000±1200 Nauplii/g cyst in the evidence with tap water and 27±5 % and 31000±1000 in varzaneh drainage water and 13±5 % and 11000±1000 in Sian drainage waters, respectively. Then, all hatched nauplii were cultured in 3 aquarium containing each 100 liters together with one evidence with 3 replicates containing 50 liters in each aquarium in laboratory. Nauplii were fed using a suspension with rice bran, Dunaliella and Naunochlorepsis with density of 2 ×10^ 6 cell/ml. The growth of Artemia Nauplii was slow at the first week of culture and then continued with suitable norm and lower mortality until maturation. The mean size of Nauplii length reached to 9 mm after 2 weeks. Their fecundity was 50 cyst per female. The drainage waters of studied sites can be used as a suitable water source at semi- industrial Artemia production pilot in the region.
    Keywords: Aquaculture ; Iran ; Esfahan province ; Artemia urmiana ; Drainage ; Artemia culture ; Propagation ; Laboratory ; Culture
    Repository Name: AquaDocs
    Type: monograph
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    Format: application/pdf
    Format: 117
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25384 | 18721 | 2018-09-14 07:53:44 | 25384 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Intensive culture of rainbow trout, usually done using several different methods. The objective of this study was to compare fish performance and welfare in a new design of Foster-lucas ponds (FLP) and a raceway ponds (RP) under field conditions. Currennt study was designed in two treatment with three repeats. Each pond was randomly stocked by rainbow trout fish with initial average weight of approximately 51±5 g. stocking density was 120n/m3 during study. After 10 months of trial, fish fed diets with commercial diet (Chineh Company). Feeding operation conducted according to the manuals by consideration of water temperature, fish weight and biomass. During the 10 months experiment, the fish survival rate was high (97.39 and 98.05%). No significant differences in growth parameters were observed between RP and FLP, but in all parameters FLP was higher than RP Final weight was 0.99% higher in FLP than in RP. Water quality factors (Dissolved oxygen, PH) was better in FLP. The results confirm that when water quality is maintained in safe level ranges, growth rate of rainbow trout attained in FLS are comparable to those in RP.
    Keywords: Aquaculture ; Iran ; Rainbow trout ; Oncorhynchus mykiss ; Fish culture ; New design of Foster-lucas pond ; Raceway ponds ; Culture ; Stocking ; Density ; Diet ; Feeding ; Temperature ; Biomass ; Growth ; Dissolved oxygen ; pH
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25391 | 18721 | 2018-09-20 11:27:50 | 25391 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Cochlodinium is a dinoflagel that, with their bloom in the coastal water of worlds, can case fish mortality. Cochlodinium was detected in Persian Gulf, since 2008. After purification of C. polykricoides with light, the culture of cochlodinium, with medium of f/2 (modified), Guilard (f/2-Si), TMRL, Conway, ESM and the mixed of sea shore sediment with salt water (32 ppt) was done. The culture of cochlodinium in test tube, erlanmeyer with volume of 200-1000 ml and aquariums with the volume of 16 liter (each with 10 liter water) with three replicates in each treatment were done. Only the culture of cochlodinium with f/2 (modyfied) was possible in the aquarium. The culture of cochlodinium with TMRL medium in aquarium was not desirable and only in one aquarium from 3 aquariums, the density of cochlodinium reached to 910.000. cell per liter. The use of f/2 (modyfied) medium in the test tubes, and erlanmeyers, with the volume of 100-1000 ml, lead to suitable cochlodinium bloom. The maximum cell density in total treatments (with 3 replicates in each treatment) in test tube 3.283.000. cell (in 15 days), in erlanmeyer with 200 ml volume, 6.393.000. cells (in 22 days), in erlanmeyer with 500 ml volume, 2.833.000. cells (in 40 days) and in aquarium 3.600 .000. cells (in 46 days) counted. Cochlodinium culture with TMRL Medium in test tube and erlanmeyer was done successfully, but with the increase of erlanmeyer volume, the blooms of cochlodinium was lesser than f/2 (modyfied) medium. The maximum of cell count of TMRL Medium in test tube 2.683.000. cells (in 12 days), in erlanmeyer with 100 ml volume, 3.207.000. cells (in 36 days), in erlanmeyer with 250 ml 3.040.000. cells (in 18 days), in erlan with 1000 ml 2.325.000. cells (in 34 days) and in aquarium 550.000. cells (in 28 days) in each litre. Unsuccessful culture of cochlodinium with Conway medium, Gyllard (f/2-Si) and mixed salt water (32 ppt) with sea shore sediments, even in the test tube, indicated that these mediums weren’t suitablefor copchlodinium culture. With the increase of erlanmeyer volume, the success of ESM medium decreased. But, in test tube and in erlanmeye with 100 ml volum, was almost, successful. We can concluded that, cochlodinium culture required, special condition and medium, that in f/2 (modyfied) medium the culture of cochlodinium was successful. The maximum cochlodinium cell count was 6.5 million per liter in one aquarium. The mean of 3 replicate of this treatment was 3.600.000. cells per liter with f/2 (modified) medium. For culture of cochlodinium polykricoides, the f/2 (modyfied) medium was better than others mediums.
    Keywords: Ecology ; Iran ; Persian Gulf ; Cochlodinium ; Different culture mediums ; Cell density ; Laboratory condition ; Purification ; Cochlodinium.sp ; Dinoflagel ; Mortality ; C. polykricoides ; Culture ; Aquarium ; Sediments ; Cochlodinium polykricoides
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 48
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