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  • Shrimp  (89)
  • Samples  (48)
  • Chemistry
  • Tehran, Iran  (137)
  • Massachusetts Institute of Technology and Woods Hole Oceanographic Institution  (1)
  • CONyMA
  • 2015-2019  (138)
  • 2005-2009
  • 1985-1989
  • 2018  (138)
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  • 2015-2019  (138)
  • 2005-2009
  • 1985-1989
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  • 1
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    Study on some physico-chemical parameters in the southern of Caspian Sea- Mazandarn Province (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This study was conducted to determine physico-chemical characteristics of water and their spatial and temporal fluctuations in the Mazandaran coastal of Caspian Sea in 6 months, four seasons at 4 transects (Tonekabon, Nowshahr, Babolsar, Amirabad) during 2012-2013. 72 samples were collected at surface layer of water in 5, 15 and 30m depths. Then the sampls analyzed based on standard methods. Results showed that the mean of water and air temperature were 19.46±0.85 and 20.25±0.78 ◦C, respectively. Minimum and maximum of water temperature were recorded in winter (7.00) and summer time (28.10). Mean of salinity was 12.35±0.13 ppt. The maximum salinity was recorded in summer and minimum in winter in all transects. Mean of transparency (SD) in the present study was 2.63±0.18m. The mean of pH was observed 8.51±0.02 which was higher than the pervious sampling periods. The mean of DO was observed 6.00±0.07 ml/l during sampling period. Annual concentration of dissolved inorganic nitrogen (DIN= NH4 +, NO2-, NO3-) has a fairly wide variation in diferents months and transects. Percentage of nitrogen components out of DIN were varied 6-53, 0.14-26.0 and 37.0-94.0 respectively. In this study, percentage of DIN was lower than 15% and dissolved organic nitrogen (DON) was higher than 80%. Mean of annual dissolved inorganic phosphorous (DIP) and organic phosphorous were 0.58± 0.04 and 0.48± 0.02 µM, respectively. DIP and DOP percentages recorded 54 and 46 respectively. Mean annual of dissolved silicon (DSi) obtained 9.5± 0.2 µM. Based on the results, the system was in phosphorus limitation during spring and summer but it shifted to nitrogen limitation in autuman and winter. Phytoplankton development was not limited by DSi at any seasons.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Physicochemical parameters ; Nutrients ; Samples ; Temperature ; Salinity ; Transparency ; pH ; Dissolved inorganic nitrogen ; Phytoplankton
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 70pp.
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  • 2
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    Study on brood stock management of Litopenaeus vannamei using imported post larvae (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: In order to domesticate Litopenaeus vannamei as a solution to single species shrimp aquaculture of Penaeus indicus and to reduce risk of shrimp culture in emergency cases, this study was carried out. Initially the postlarvae reared from imported L. vannamei were cultured. After culture season the adult shrimps were transported to greenhouse ponds for wintering. It is then relocated to hatchery saloon. After eye stalk ablation and maturation the female brood stocks were introduced to matured male for copulation at ponds. During the study period, male and female shrimps attained full maturation at end – April by temperature over 25ºC, the copulation process was started significantly. Finally of the 12 instances of copulation, one instance resulted nauplii production. The best maturation was observed at 28 ֯C ±1 and salinity30 to 33PPT and pH of 8 to 8.3 Fresh sea worms and squid showed the highest effect on fecundity in brood stocks. Besides, the spring season was obtained to be the best period for brood stock production in Bushehr province.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Litopenaeus vannamei ; Brood stocks ; Larvae ; Species ; Shrimp ; Aquaculture ; Postlarvae ; L.vannamei ; Hatchery ; Maturation ; Nauplii ; pH ; Sea worm ; Squid
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 25pp.
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  • 3
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    Ecological study on rearing shrimp ponds (Litopenaeus vannemei) in Choebdeh Abadan (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Following to introduce Vannamei shrimp (Litopenaeus vannamei) into Iran aquaculture industries by fisheries organization its postlarvae were successfully reared in ponds in Bushehr and Choebdeh in Khuzestan. Due to activity of Shrimp site and importance of input and output water quality, this study was done in 11 stations including: two stations in Bahmanshir river, two station in C4 and C5 irrigation canals, six staions in active farms along C4 cannel (Yonesi, Mosavei and Salman Zadeh), and C5 cannel (Mohamadi, Khairi and Ashraf Por) and one staion in output draing were selected. Sampling were carried out from May2009 befor beginning stocking to harvest time in October 2009. Physico- chemical parameters and plankton sampled biweekly and benthic animals and heavy metals were sampled monthly. Some parameters such as pH and temperature by using portable multy parameters and others. Analyzed with standard methods. Heavy metals were measured by plarography method (797 Vamtro). Plankton and benthos samples were studied only in Bahmanshir station. According to results the maximum values for DO (11.1 ppm) PO4 (1.86 ppm) NO3 (8.4 ppm) and TSS (4992 ppm), pH (8.4) NO2 (0.18 ppm) BOD5 (9.06 ppm) were measured. In comparison to aquaculture water quality standards except for TSS in river stations others are located in allowable range. We didn’t observed any main changes in water quality from river to output drainage. The results of biotic parameters in river stations showed that diatoms were the most group of phytoplanktons. Zooplankton groups were Copepods, Rotifera and Protozoa. Tintinids and Nematoda larvae were the most abundant zooplankton. Benthic animals were included nine crustacean species and two annelid species. Isopods with 52% were the most and ant group.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Ecological ; Physico-chemical ; Vannamei shrimp ; Litopenaeus vannamei ; Water quality ; Abadan aquaculture site ; Rearing ; Shrimp ; Ponds ; Sampling ; Plankton ; Heavy metals ; pH ; Temperature ; Benthos ; Phytoplankton ; Zooplankton ; Copepods ; Nematoda larvae
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 80pp.
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  • 4
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    The study of genetical differentiation of bisexual and parthenogenetic strains of Artemia urmiana by RAPD s method (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The brine shrimp Artemia consists of a number of bisexual species and a large number of parthenogenetic forms, which collectively, inhabiting a wide range of hypersaline habitats. This research was carried out in order to study the genetic differentiation of Artemia urmiana and two parthenogenetic populations originated from surrounding lagoons of the Lake Urmia (Fesendoz, Saray village) and also to find the phylogenetic relationships of some Iranian Artemia (Qom, Arak, Golestan, and Shiraz) with a comparison to some foreign Artemia species (A. sinica, A. franciscana, A. parthenogenetica (Russia and Turkmenistan) using a molecular technique (PCRRAPD`s). Of a total number of 56 random primers (10 bp) survey, 29 and 26 oligonucleotides showed polymorphic patterns with highly reproducible bands in Artemia urmiana groups and Iranian Artemia groups.The highest percentage of polymorphic loci were in Artemia urmiana (P= 78.89 %) and in comparison with the other parthenogenetic forms, in parthenogenetic Artemia from Saray village (P= 59.8 %). The highest values of Shannon index were estimated in Artemia urmiana (I= 0.41), and in comparison with other parthenogenetic forms, in parthenogenetic Artemia from Saray village (I= 0.3). Analysis of molecular variance indicated that most variation were within populations (A. urmiana with parthenogenetic forms, FST= 0.35) and among populations (Iranian Artemia with foreign species, FST= 0.76). Genetic pattern was not in accordance to geographical distribution, which indicated the wide range of Artemia samples at different regions in Iran. UPGMA showed two distinct clusters in group I including A. urmiana with two parthenogenetic forms and four clusters in group II including Iranian Artemia with some foreign species. It is understood that two parthenogenetic populations (Fesendoz and Saray village) had close genetic relationship together with high value of heterozygosity in parthenogenetic Artemia from Saray village. Iranian Artemia (Qom, Arak, and Golestan) indicated close genetic relationships with A. parthenogenetica (Russia and Turkmenistan) with low values of heterozygosity. A sister group was generated including Shiraz and USA Artemia samples which attests a possible contamination of local water with A. franciscana. FST value revealed high genetic structuring at all studied groups. Besides, it is suggested that gene flow was not strong enough to prevent substantial genetic differentiation. Result could lead to a management programmes to use suitable Artemia strains with high genetic diversity, for culturing and establishment of gene bank of Artemia stocks in Lake Urmia.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Artemia urmiana ; Parthenogenetic populations ; Iranian Artemia ; Phylogenetic relationships ; RAPD`s ; Shrimp ; Bisexual species ; Phylogenetic ; A. sinica ; A. franciscana ; A. parthenogenetica ; Survey ; A. urmiana
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 56pp.
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  • 5
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    Effects of freezing duration, temperature and storage conditions on accumulation (distribution and redistribution) of trace elements in different tissues of two shrimp species from the Persian Gulf (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This study investigates the distribution of three trace elements (Cd, Cu and Zn) in tissues (exoskeleton, abdominal muscle and hepatopancreas) of two shrimp species (Penaeus merguiensis and Penaeus semisulcatus). Special emphasis in this study was placed on evaluating the effects of storage conditions on redistribution of the elements and moreover, the potential roles of metallothionein in tissue redistribution. The specimens were collected from the northern part of Persian Gulf during August 2001 and September 2002. The concentrations of metals in the tissues were measured using an Inductively Coupled Plasma-Atomic Emission Spectrophotometer (ICP-AES) and an ICP-Mass Spectrometer (ICP-MS). Metallothionein levels were determined using Differential Pulse Polarography. Results show a similar hierarchy for Cd and Cu concentrations among different tissues as follows: hepatopancreas〉 exoskeleton〉 muscle; and for Zn, the hepatopancreas again contained the highest levels, though there were not any significant differences in Zn concentrations between muscle and exoskeleton. Significant differences were found between the species in the case of Cd and Zn concentrations. Cd and Zn levels in P. semisulcatus were markedly higher than in P. merguiensis. In general, there were highly significant differences among the storage conditions from accumulation the selected trace elements and metallothionein in both species point of view, but in most cases no significant differences could be observed among the fresh, refrigerated and frozen specimens of P. semisulcatus. In regards to Zn and Cu accumulation in edible (muscle) tissues of P. merguiensis, optimum conditions for frozen storage is -30ºC as ice frozen, and in terms of short term storage, one day refrigeration is recommended. The redistribution of Zn and Cu during the storage period can be attributed to the role of metallothionein in their transport from organ(s) to muscle during storage. The variations in pH values measured in the muscle of P. merguiensis in different storage conditions showed a similar trend with metallothionein, Zn and Cu. In contrast it appears that cadmium is transported from muscle to the other tissues. Exoskeleton is probably one of the recipient tissues of cadmium. The inter-elemental relationships were assessed for each species, separately. A significant positive relationship was observed between the concentrations of Zn and metallothionein in P. semisulcatus, whereas in P. merguiensis, Cd concentrations decreased significantly with increasing metallothionein. Highly significant differences between the sexes in Cu and Zn contents could be detected. Highly significant size-related differences were found in the levels of Cd, Cu and metallothionein in the selected tissues of the shrimp species. Comparison between the mean concentrations of the elements in muscle tissue and existing guidelines indicate that in nearly all cases the concentrations were well below permissible levels for human consumption. The only exception was observed regarding mean values of Cd, which was somewhat greater than some of the recommended levels.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Heavy metals ; Distribution ; Redistribution ; Shrimp ; Metallothionein ; Storage conditions ; Tissues
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 50pp.
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  • 6
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    Determination of catchability bony-fish and optimized exploitation in the Caspian Sea (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: To accomplish the stock assessment that pre-planned for bony-fish resources investigation started by considering two pivotal act. First gathering biometric data and second using catch statistics information for stock analyses. Implementing the research programmes five itinerant groups in Anzali, Keyashahr, Noshahar, Babolsar, Torkaman have been organized during fishing season. The biometric data were supplied in computer that distincted by each fish species and subdivided into age groups. In analyses the samples, Bertalanffy formula used for calculating average length and age, also for growth coefficient. Total mortality rate calculated by using Pauly formula, and the biomass status estimated through Cohort analysis. The catch by beach seiner cooperatives recorded 8630 metric tons, but estimation including. Poacher catch raised the harvest to 15.6 thousand tons. The total catch of Kutum reduced by 1400 tons compared with previous year, but the estimation revealed the biomass of Kutum 18.5 thousand tones, the stock stowed reduction in quantity relative to past years. Harvest exceeding the limit, fishing pressure. And employing higher fishing effort, combining releasing the Kutum seedling with shorter average length in recent years, leaded the shrinkage of biomass of Kutum resources. The Golden Grey mullet catch has been estimated 3437 metric tons, the figure showed 1500 tons increment, in comparison with previous year, nearly 24% of catch was shorter than Lm50% of this species. In Guilan province higher number of sub - size golden mullet captured, this development occured less in Mazandaran and Golestan province. Higher rate of exploitation, reduction of catch in recent years, dominating small size fish in catch composition indicated the population undergoing stress in ecosystem. Thin - lipped grey mullet only comprised 9.2% of the mullet catch - Length - structure of this fish species population formed mainly by young small size fish, the biomass of latter estimated 1100 metric tons. Catch further than maximum sustainable yield, by higher exploitation rate, and gradual diminishing of harvest, represented the resources of this fish species suffering both by high fishing pressure and un - sustainable effort, which ultimately declined the fishery of this fish species. Carp catch and its resources in Anzali region become very low, it is necessary to enhance the stock by Sea-ranching programmes. The carp resources in Gorgan region enjoy better status, but catch practices do not compatible with rule and regulation in Gorgan and Gomeyshan area. The standing stock of Gorgan carp estimated nearly 6000 tons. The information concerning carp resources indicate the stock experiencing pressure and likely the catch will diminish in future. The total catal catch of carp calculated 1900 tons. In recent years the Caspian roach resources are increasing but the bulk of harvest fished illegally and by gill - nets. The total catch of the roach during 1998-1999 estimated 1700 tons. Considering the semi - artificial propagation of roach in Golestan province, if has been concluded the multiplication of this fish species also should be planned by fisheries authorities for Anzali region. It is also advisable catching of this fish species to be regulated soon and selective catch are proposed. The catch of pike - perch during the year of 1997 - 98 jumped to 95 tons, but 90% of catch formed by young fish or sub – standard length size. Almost, all the increment of catch attributed to the releasing of millions of seedlings in the Sea which the plan has been implemented by Iranian Fisheries. Increasing the numbers of released seedlings, with higher average weight Identification of most suitable site for unloading the fries could ensure better survival rate and contribute the enhancement of resources and produce good catch for this desirable fish species. The total catch of Bream hit 18 tons, but most of the catch comprised by young fish with sub - standard size. The present formation of fisheries population of bream are the result of seedling releasing! In the Sea by Iranian Fisheries, since the catch of bream are negligible despite releasing programmes of seedling in a decade which 14million individuals until now, one could conclude the programmes do not contributed effectively to stock rehabilitation. The Caspian Barbus (Barbus brachycephalus) are at the threshold 01 vanishing, at present a few numbers are caught, therefore B.capito appeared in catch composition. The catch of latter species in recent two years are increasing. Total catch during the year of 1998 - 99 estimated 34.5 tons, biomass calculated 93 tons. Caspian vimba are small size fish which beach seiner (mesh size in bosom 30 millimeters) could not capture them ideally, bulk of the catch caught in illegal manner by gill nets. The total catch estimated 154 tons which the present fishing procedures surpassed total allowable catch (136 tons). In recent years the catch of this fish species are increasing. "Shemaya' similar in size with Vimba are caught less by beach seiner, illegal catch are considerable, the total catch in 1998 - 99 estimated 86 tons. The level of harvesting of this fish species are increasing in recent years and its population size become significant relative to last years. The main part of catch concerning Aspius fish species occured in Guilan coastal area, high fishing pressure reduced the population size, including low discharge of water in rivers during spawning period, impact negatively to reproduction. Catch statistic in 1989 showed 128 tons of this fish species are caught but in 1998 - 99 it has been reduced to 6.8 tons. It has been suggested artificial spawning could prevent its fate as endangering species. Caspian salmon catch estimated 78 tons during 1998 - 99, salmon population in Caspian Sea are supported by releasing reared smolts, which in recent years nearly 500 thousands fingerlings released in suitable rivers. After banning the gil nets application in Southern Caspian Sea, the catch of salmon in beach seining are increasing. It is necessary the released smolts in rivers being protected for its safe approach to Caspian Sea. The total catch of herring fish species in 1998 - 99 were nearly 700 tons. The bulk of the catch occur In Guilan fishing area, elimination of gill - nets usage in Southern Caspian Sea contributed the increment of herrings catch in beach seining. The herring resources in Caspian Sea reduced relative to past decades. The catch during 1913 – 1916 ranged between 130 - 160 thousand tons, in Caspian Sea.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Stock assessment ; Bony-fish ; Fishing ; Species ; Samples ; Growth ; Mortality rates ; Biomass ; Kutum ; Carp ; Survival rate ; Barbus brachycephalus ; B.capito ; Population
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 136pp.
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  • 7
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    Assessment of environmental conditions of some important shrimp habitats in the east and west of Hormozgan province (Tiab, Yekshoveh, Laft and Khamir Estuaries) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Protecting coastal aquaculture systems as a habitat for aquatic systems is one of the serious concerns of the world. In the present study, spatial and temporal variations of Some physicochemical parameters, nutrient concentration and water quality In some estuaries of East and West of Hormozgan province were investigated from March 2015 to February 2016.Data analysis was performed using multivariate statistical methods (such as: CA,DA and PCA Analysis) and Eutrophication and water quality index using multivariable indices. The average annual variation of various parameters studied in West Azarbaijan province of Hormozgan is as follows:water temperature (18.1-34.6Co ), Salinity (35-45.6 ppt), Dissolved Oxygen (4.6-7.4 mg.l), pH (8.01-8.4), Nitrate (32.5- 403.5µmo.l), Nitrite (3.6-50.7 µmo.l), Total Ammonia (9.8-311.2 µmo.l), phosphate (22-569.1.6 µmo.l), and chlorophyll-a is equal to(0.46-4.04 mg.m3 ), respectively.Also, the mean annual variation of the various parameters studied in the eastern province of Hormozgan has been as follows: water temperature (18.1-34.6Co ), Salinity (35-45.6 ppt), Dissolved Oxygen (4.6-7.4 mg.l), pH (8.01-8.4), Nitrate (32.5-403.5µmo.l), Nitrite (3.6- 50.7 µmo.l), Total Ammonia (9.8-311.2 µmo.l), phosphate (22-569.1.6 µmo.l), and chlorophyll-a is equal to(0.46-4.04 mg.m3 ), respectively. One-Way Analysis of Variance test showed that the significant for some differences physicochemical parameters varied seasonally.Discriminant analysis, water quality conditions in terms of location classified into three groups.The analysis of PCA showed that the amount of nitrate and total ammonia in the tiba and phosphate in Laft and Kgamir Stricks with two sources (domestic and agricultural waste) and natural sources, can be considered as the main factors affecting the intensity of Eutrofication.The range of trophic Trix and TI eutrophication index in the studied areas were 4.3-6.1 and 3.5-4.2 respectively.According to on the indicators of and TI index, trophic status was classified into mesotrophic(in laft and khamir areas), Oligo-Mezotrophic(Yekshoveh area) and mezo- eutrophic respectively. Also Based on Trix index, the Water quality status was from bad condition in tiab area and good condition in laft, khamir and yekshoveh areas respectively. The determination of trophic conditions with a single-variable method based on chlorophyll a and Total inorganic Dissolved materials(DIN) showed a Very good agreement with multivariate trophic indices.The range of changes in some of the parameters studied in this study was more than the reported limit for the conservation of aquatic life in coastal areas.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Environmental ; Shrimp ; Habitats
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 76pp.
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  • 8
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    Investigation on feeding of Sardines and major Tuna fishes in west of Oman Sea (Jask area) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Feeding habits of three dominant species of tuna fishes (Thunnus tonggol, Thunnus albacares, Euthynnus affinis) and dominant species of sardine (Sardinella sindensis) were investigated from December 2006 to November 2007 in the western waters of Oman Sea. Seasonally samples of three species of these tuna and monthly samples of sardine were collected. Feeding studies on 101 longtail tuna (T.tonggol) indicated that the bony fishes were main food of this species (98.3%) and followed by random foods included cephalopods (1.7%) and crustaceans (1.7%). Among the bony fishes, anchovy (66 %) and sardines (25.9 %) were most important than the other fishes. FI and CV for this species were calculated 26.4 and 21.8 respectively. Stomach content analysis of 85 specimens of E.affinis demonstrated that bony fishes were only food for this species (100%). Sardines (49.3%) and anchovy (11.9%) were foremost food items for E.affinis. Moreover FI and CV were calculated 64.7 and 15.3 correspondingly. The stomach of 120 yellowfin tuna (T.albacares) were analyze. Results showed that bony fishes,cephalopods and crustaceans were formed food items of this species .Yellowfin tuna were preferable fed on bony fishes (87.7%) specially sardines(41.2%),flying fish(13.9%)and M.cordyla(10.4%) and followed by cephalopoda (43.8%) specifically Loligo sp.(84.5%) and crustaceans(11.2%) . FI and CV were calculated 38.3 and 25.8.Study on 320 stomachs were determined that zooplankton and phytoplankton formed food of S.sindensis. Zooplankton (crustacean and molluscs) were main food (90%) and phytoplankton (Bacillariophyceae and Dynophyceae) were least food items (10%). Copepoda (crustaceans) with 58% were preferable food and bivalves and after that bivalves (molluscs) with 14%, Bacillariophyceae (8%) and Cyanophyceae (2%) CV and FI were 30.3 and 29.4 respectively.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Feeding Habits ; Stomach content ; Thunnus albacares ; Thunnus tonggol ; Euthynnus affinis ; Sardinella sindensis ; Species ; Tuna ; Samples ; T.tonggol ; Cephalopods ; Crustaceans ; Anchovy ; Phytoplankton ; Zooplankton
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 124pp.
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  • 9
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    Population genetic study on two commercial shrimp species and determining of DNA-Barcoding of 6 – 8 species by partial sequencing of mtDNA using molecular method (PCR-Sequencing) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Barcodes are short segments of DNA that can be used to uniquely identify an unknown specimen to species, particularly when diagnostic morphological features are absent. These sequences could offer a new forensic tool in plant and animal conservation especially for endangered species. Ideally, barcodes could be used to positively identify illegally obtained material even in cases where diagnostic features have been purposefully removed or to release confiscated organisms into the proper breeding population. In the present study molecular data were used besides the morphological ones to introduce a specific barcode for six selected species of Shrimp. Based on the previous phylogenetics study, it has been proved that the mitochondrial COI gene in crustacean is a good discriminative marker at both inter- and intra-specific levels. In this regard, six species of shrimp (Fenoro penaeus indicus, Fenoro penaeus merguensis, penaeus semisulcatus, Metapenaeus affinis, Marsupenaeus japonicas, Fenoro penaeus penicillatus were collected from the Persian Gulf and Oman sea. After identification, the total DNA was extracted; COI gene was first amplified and then sequenced for each species. Finally the collected data were analyzed with the specific phylogenetic software. Molecular analysis revealed some degree of interpopulation differences but six good species were recognizable based on COI sequences. Also for population study molecular data of two species Penaeus semisulcatus and Metapenaeus affinis were analysed base on COI RFLP and 16SrRNA sequences respectively. The results would be helpful to protect shrimp species.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Commercial ; Morphological ; Population ; Genetic ; Shrimp ; Species ; DNA barcoding ; Partial sequencing ; mtDNA ; Molecular method ; PCR ; Fenoro penaeus indicus ; Fenoro penaeus merguensis ; Penaeus Semisulcatus ; Metapenaeus affinis ; Marsupenaeus japonicas ; Fenoro penaeus penicillatus
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 74pp.
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  • 10
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    Effects of added substrate on growth and survival of juvenile Indian white shrimp (Penaeus indicus H. Milne Edwards) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The effects of added artificial substrates on juvenile white Indian shrimps (Penaeus indicus) were evaluated. .....
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Shrimp ; Aquaculture ; Juveniles ; Feed conversation ratio
    Repository Name: AquaDocs
    Type: Report , Not Known
    Format: 61pp.
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  • 11
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    The effects of unsaturated fat acids on reproductive indicators of penaeus indicus (Phase 1: Preparing brood of prepared juvenile shrimp of sea and pond in the controlled conditions by using natural feed) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The shrimp farming success in the future will greatly depend upon increasing supplies of healthy and high quality seed for stocking ponds. The limited and inconsistent availability of wild shrimp postlarvae together with an urgent need of establishing selective breeding programs, increased the interest in captive broods of penaeid. Many investigations have been carried out on several shrimp species world-wide. This study have been conducted on 2 shrimp groups since September (Mehr) to July (Tir) 1999 in Bandar-e-Imam southwest Iran. The wild group (SPB) prepared from Jask area and the pond group (POB) prepared from artificial ponds upon harvesting them as marketable size have been investigated. 2 groups were exposed to natural diets consisting of squid and artemia biomass in same conditions using 12 m3 concrete tanks. Stocking density was 2 piece/m2 (1F:1M). Half part of the pond bottom covered by fine sand to reduce the stress where the other part has been used for feeding. Daily ration given in 3 times by satiation method. Also reproductive performance of wild gravid brooders (WLB) were recorded as a control group. 2 examined groups well grown and around 50% of the SPM and around 30% of POB females successfully ripened a produced eggs and nauplii where almost all males of both groups were matured. Data showed fecundity is highly correlated to the TL and W of the brooders by positive trend. Considering the TL, W, Fecundity and nauplii production the WLB brooders are significantly (P=0.05) higher than 2 other groups, while the SPM broods are significantly (P=0.05) higher in hatchability. Meanwhile the survival rate from nauplii to postlarvae stage was around 30% for SMP brooders. On the other hand the growth parameters (TL, W) of the marketable size of the shrimps produced from SMP brooders were significantly (P=0.05) higher than those produced from WLB group. Since the fecundity is improvable by increasing the brood size thus the data suggest the sup.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Fatty acids ; Penaeus indicus ; Brood ; Juvenile ; Shrimp ; Postlarvae ; Species ; Squid ; Artemia ; Biomass ; Nauplii ; Fecundity
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 42pp.
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  • 12
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    Survey on health status in aquaculture Sturgeons Centers( Mazandaran, Guilan And Golestan Provinces) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Study of survey health management and diseases in hatcheries and fish farms can help us to knowledge and application control methods such as: prevention, treatment and increase high levels of production in hatchery and farms, finally. This survey carried out from 2005 to 2008 for 4 years in sturgeon hatcheries and farms of Golestan province. Sturgeon fishes include Huso Huso, Ship sturgeon, Acipenser persicus collected and for virology, bacteriology, fungius and hematology examined. Also, physicochemical parameters measured and recorded in different stages of culture. Results of this study showed that all of samples in virology was negative and did not observe any doubetful causes. In bacteriology CFU was variation from 3/9 ×105 to 6/9×10. The most parasites that detected in this survey was Cocolanus espherolanus , Sceria binopsulus semiarmatus and Amphilina fuliacea that separates from Acipenser Percicus, especially. The results about hematology parameters some important hematological indices of ship sturgeon include: The total RBC for female and mail specimens measured as 5.3±1.5 ×105, 4.8±0.5 ×105 per mm 3 respectively. The amount of haematocrit and hemoglobin for female and mail determined: 34.3±2.8, 35±1.4 percent and 10.3±0.9, 8.9±0.8 gr/dl .The MCV: 216.3± 96.2, 736.5± 102.5, MCH: 720.2±309.5, 186±0.7 and MCHC: 30±0.8, 25.5±3.4 percent respectively.The total WBC were (female, male): 21320±1054, 20580±777 per mm 3 and neutrophil: 16.4±2.5, 17±1.4 percent and lymphocyte: 74.4±2.4, 73.5± 0.7 percent and eosinophil: 6±1.4, 6.4±0.5 percent, monocyte: 2.8±0.8, 3.5±0.7 percent. There was not any significant differences (p〉0.05) between mentioned parameters in male and female (students t-test). Also evaluation of hematological parameters in bluga ( Huso huso) include: total RBC were (male , female) 5±0.3 ×105 , 4.9±0.6 ×105 per mm 3 ,respectively and hematocrit: 33.2±6.7 , 35.4±3.4 percent and hemoglobin: 11.2±1.5 , 12.2±1gr/dl and MCV: 669.9±172.2, 723.9±982.4 and MCH: 226.2±42.5, 249.5±35.4 and MCHC: 34.1±2.4, 34.6±3.6 percent respectively. The totals WBC were (male, female): 24800±707.1, 23042±1375.4 per mm 3 and neutrophil: 18.5±0.7, 21.4±1.1 percent and lymphocyte: 73.5±1.4, 68.4±1.1 percent and eosinophil: 5±2.8, 7±1.2 percent and monocyte: 3.5±3.5, 3.2±0.8 percent. According to statistically study the count of lymphocyte had significant difference between male and female fish and this count in male was higher than female. (p≥0.05).
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Physicochemical ; Hematology ; Fish ; Sturgeon ; Huso huso ; Ship ; Acipenser percicus ; Bacteriology ; Parasitology ; Health management ; Diseases ; Survey ; Aquaculture ; Hatcheries ; Samples ; Sceria binopsulus ; Amphilina fuliacea ; Female ; Male
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 395pp.
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  • 13
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    Development gene data bank of cultured shrimp species and their indigenous pathogens in Iran (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Protection authorities of plant and animal genetic resources and conservation of biodiversity as national assets by using biotechnology techniques is the most important objectives of genetic data information bank. Establishment of shrimp species genetic data bank, makes possibility of study multiple genes such as economic genes and since knowledge of genetic variation within and among populations and barcoding species of shrimp are very important for species conservation and with so little researches of Asia in the global studies of DNA barcoding and animal taxonomy in Iran, In this study, Iran shrimp genetic diversity and genetic data bank was done. DNA barcode is a short, standard well known sequence of cytochrome oxidase І gene. By using this DNA sequence can be realized that each animal, plant or fungus belongs to which species. Also prepare a bank of genetic data shrimp pathogens and maintenance of these strains are steps to advance future researches in the fields of pathogenesis mechanism of pathogens, diagnosis, treatment, Disease prevention, production diagnosis native kits and detection of emerging and reemerging diseases its origins. So in this plan samples were collected from imported cultured shrimp Litopenaeus vannamei and and 6 Persian Gulf and Oman Sea shrimp species which classified based on traditional systematically as: Penaeus semisulcatus, Fenneropenaeus merguiensis, Metapenaeus affinis, Parapenaeopsis Stylifera and Fenneropenaeus indicus. After examination of DNA barcode sequence, molecular and bioinformatics operations of each sequence in the Consortium for the Barcode of Life (CBOL) and National Center for Biotechnology Information (NCBI), phylogenetic analysis of each sample was determined and similarity of each sample with NCBI and CBOL database was checked and the closest species to each sample were specified. According to the results different samples of L. vannamei, . banded P.semisulcatus, F. merguiensis and F. indicus have more than 97% similarity to the same species of other countries. non banded P.semisulcatus had 80.07% similarity to banded P.semisulcatus, M. affinis samples had 90.3% similarity to Metapenaeus ensis and Parap. Stylifera had 93.44% similarity to Parapenaeopsis coromandelica in the CBOL. This funding confirmed the need for further investigation and possible announcement of new species. By using ribotyping technique, native isolated pathogenic bacteria and fungi were identified and recorded in the gene bank database center. During sampling of shrimp and water of Specific Pathogen Free shrimp center, 40 bacterial strains were isolated, which 8 of them had the most frequency and identification based on 16S rDNA sequencing was performed. Bacteria identified are: Vibrio nigripulchritudo strain IS013(GenBank:KP843725) ،Vibrio brasiliensis strain IS014 (GenBank:KR186076) ،Vibrio rotiferianus strain IS015 (GenBank:KR186077) ،Vibrio azureus strain IS012 (GenBank:KJ018724.1) ،Vibrio owensii strain IS016 (GenBank:KR186078) ،Agarivorans gilvus strain IS017 (GenBank:KR186079) ،Vibrio brasiliensis IS018 (GenBank:KR186080) and Vibrio alginolyticus strain IS019 (GenBank:1817854), which were recorded in The World Bank genes. In this study fungal isolates were not detected.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Bacteria ; Ribotyping ; 16S rDNA ; Shrimp ; DNA barcode ; GenBank ; Vibrio nigripulchritudo ; Vibrio rotiferianus ; Vibrio owensii ; Vibrio brasiliensis ; Vibrio azureus ; Agarivorans gilvus ; Vibrio alginolyticus ; Parapenaeopsis coromandelica ; Metapenaeus ensis ; Litopenaeus vannamei
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 114pp.
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  • 14
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    Population Genetic Structure of Neogobius caspius (Eichwald ,1831) in the east and central of the South Caspian Sea using Microsatellite Markers (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Neogobius caspius is a small benthic fish that is native to the Caspian Sea. The importance of this fish is because of it is role as a main food resource of the Sturgeon fish. The genetic diversity of N.caspius populations in the Caspian Sea was studied using microsatellite technique. In the study 95 Samples of Neogobius caspius from two regions (torkman port and Chalus) in south Caspian Sea were collected. DNA was extracted using 12 pairs of microstellite primers polymerase chain Reaction (PCR) was conducted. DNA Bands were analyzed using uvdoct and GenAlEx software package. Out of 12 microsatellite primers, 11 loci were produced, in which 5 of them were polymorphic ,6 monomorph and one of them showed smear. Average of observed and expected hetrozygosity was 0/798 and 0/843 respectively. Significant genetic differences between 2 regions was observed (P 0/01). Deviations from hardy- Weinberg equilibrium were in all cases. These results indicate that at least, 2 population of Neogobius caspius exist in the south Caspian sea.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Microsatellite ; Genetic ; Population ; Neogobius caspius ; Benthic ; Native ; Sturgeon ; Diversity ; Samples ; PCR
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 22pp.
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  • 15
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    Monitoring of use possibility of edible films ( whey protein, sodium alginate and mix of them) for cleaned Kilka packaging (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This project was carried out in order to increasing of nutritional value, taste and shelf life of cleaned Kilka Fish during cooled storage. Edible films made by Whey protein and Sodium alginate were used for fish packaging. This search carried out in two stages consisting of pre- study and study. 3, 6, 9 and 12% concentrations of Wp and 0.5, 1, 1.5 and 2% concentrations of SA at three times including 0, 2 and 4 hours were used in pre-study stage. The covered samples were kept in -18 ֯C. Microbial and sensory examination were carried out for a period of two months. Microbial factors were including total bacterial count, Staphylococcus bacteria count, Coliform, Escherichia coli and Pseudomonas bacteria. Sensory tests consist of taste, odor, color and tissue were studied in the fish samples. 12% and 0.5 % concentrations at time = 0 of edible films made by WP and SA considered in study stage. This is can be due to the significant differences in total acceptance index of sensory tests. Control sample cleaned Kilka was packaged in disposable dishes with cellophane covers in 500gr in weight. Two selected timar and mixed cover including 12 % and 0.5 % concentrations at time = 0 of edible films by WP and SA considered in study stage. The covered samples were kept in -18 ֯C. Microbial, chemical and sensory examination were carried out for a period of six months. These factors and chemical factors consisting of humidity, protein, lipid, ash, calorie, Peroxide value, free fatty acids, thiobarbitoric acid, TVN and pH were studied in test samples compared with the control samples. Coliform, Escherichia coli and Pseudomonas bacteria contamination were negative until the end of storage period in the covered samples. The mean total bacterial count and Staphylococcus bacteria counts in processed samples by WP were 2.47 and 1.61 logcfu/g, in processed samples by SA were 2.84 and 1.28 logcfu/g, in processed samples by WPSA were 2.51 and 1.44 logcfu/g, and in control samples 4.11 and 2.93 logcfu/g from 1 day until six months after processing, respectively. The mean of moisture, peroxide value, TVN, pH, free fatty acids, thiobarbitouric acid , protein, fat, ash and calorie in the covered samples by WP were 73.91%, 0.13 meq/kgoil, 9.84mg/100g, 6.15, 1.15gr/100, 0/006 mg/kg, 19.00%, 4.25%, 2.1% and 120.73 kcal/kg, in the covered samples by SA were 73.91%, 0.06 meq/kgoil, 9.84mg/100g, 6.15, 1.15gr/100, 0/006 mg/kg, 18.85%, 4.72 %, 1.90 % and 125.98 kcal/kg, in the covered samples by WPSA were 73.91%, 0.06 meq/kgoil, 9.84mg/100g, 6.15, 1.15gr/100, 0/006 mg/kg, 18.50 %, 4. 65 %, 2.25 % and 126.48 kcal/kg and in control samples 59.43%, 3.25 meq/kgoil, 16.22mg/100gr, 6.71, 9.21gr/100, 0/15mg/kg, 18.2%, 4.00%, 1.80% and 107.10 kcal/kg, respectively. No statistically significant differences were observed in the results of chemical experiments of the covered samples and presence of the meaningful difference at the results of the chemical experiments of the control sample, The covered samples by WP, SA and WPSA up to the end of storage period at cold-room had a favorite quality but the control samples had lost their. No statistically significant differences were observed in the WP samples compared with the WPSA samples (p〉 0.05). Samples covered by SA had better quality compared with other samples which can be due to the presence of the significant difference in total acceptance index among covered samples without considering of economical worth.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Nutritional ; Chemical ; Economical ; Edible films ; Kilka packaging ; Quality evaluation ; Cooled storage keeping ; Chemical analysis ; Protein ; Sodium ; Samples ; Bacterial ; Protein ; Lipid ; Fatty acids ; Escherichia coli ; Pseudomonas bacteria ; pH
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 109pp.
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  • 16
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    The Creation of genetic basic population of Rainbow trout((Onchorhynchus mykiss) based on Study of genetic variation in brood stocks using microsatellite (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: In order to perform the project, 446 samples of rainbow trout (Onchorhynchus mykiss) from 24 different regions in Iran were collected. About 2-3 g of caudal fin samples was collected from each specimen and preserved in absolute ethyl alcohol and then transferred to the genetic laboratory. Genomic DNA was extracted using the phenol-chloroform method and then DNA content and quality was determined using spectrophotometry and agarose gel electrophoresis, respectively. Polymerase Chain Reaction (PCR) of genomic DNA fin samples was carried out using 10 pairs of microsatellite primers. All PCR products were electrophoresed on 6% polyacrylamide gel and stained with silver nitrate. Following the scoring of alleles, all parameters including allelic frequency, effective number of allele, observed and expected heterozygosity, shanon index, measurement of similarity and genetic distance and Hardy-Weinberg equilibrium, Fst , Rst and gene flow were calculated using AMOVA analysis in the GenAlex and Popgene programs. The results showed that 8 pairs of microsatellite primers were polymorphic. In total, 50 alleles were determined with the range size of 64-280 bp. The locus omyf had maximum number of allele (26) and loci OTSG 474 and Strurruta58 had minimum number of allele (5). The observed heterozygosity was between 0.86 and 0.964. Hardy-Weinberg departure was observed for all loci from farms 18, 15, 4, E20 and 21 and were disequilibrium (P〈0.05). The farms 14, 8, 7 and 6 were equilibrium at 3 loci, but showed disequilibrium in other loci. The other farms were equilibrium at 1 or 2 loci and disequilibrium at 8 or 9 loci. The FST results showed that maximum FST (0.24) were between farms 1 and 11in which had minimum of gene flow (3.7). Minimum FST (0.04) were between farms 8 and 9 in which had maximum of gene flow (346). Based on the results of AMOVA analysis, significant differences were detected between all farms (P〈0.01). Furthermore, based on Nei 's standard (1972) maximum genetic distance (0.89) were observed between farms 2 and 11 and maximum genetic similarity (0.15) were detected between farms 3 and 4. This result suggests that the unique genetic variation of rainbow trout in hatchery farms of Iran represents a highly valuable genetic resource and provide useful information for creating a based population in the future breeding programs.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Genetic ; Population ; Rainbow trout ; Onchorhynchus mykiss ; Brood stocks ; Microsatellite ; Samples ; Specimens ; DNA ; PCR ; ANOVA
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 78pp.
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  • 17
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    A survey on effects of shrimp aquaculture on the coastal waters of Bushehr (Helleh and Mond) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The effects of aquaculture industry on the environment were evaluated by studying the water quality of Bushehr costal water during culture season 2005 and 2006. The variations of selected parameters such as total phosphorus, ammonia, chlorophyll a, pH, salinity and... Were monitored in effluent canal, influent canal and open sea in Helleh and Mond regions as monthly. Following occurrence of White Spot Disease (W.S.D) in the shrimp ponds of Bushehr province, shrimp culture suspended and entrance of effluent waters completely were blocked since August 2005, despite the absence of aquaculture sewage evaluation of environment and sampling were continued. During releasing of sewage of shrimp ponds to the coastal waters of Bushehr in Jun 2005, the average amount of ammonia in Helleh and Mond were obtained to be 0.161 mg/l and 0.194 mg/l, however the average amount of total phosphorus in Helleh and Mond regions were 0.149 mg/l and 0.043 mg/l, respectively. Although effluent water suspension, amount of ammonia and total phosphorus were increased in both region especially in June 2006. Comparing present data in Jun 2005 and recorded data from culture period 1997-2003 with permitable range of municipal and aquaculture waste show that; aquaculture industry on both regions have not had any obvious negative effect on the coastal waters of Bushehr but it seems if other sources of pollutions in the region is not controlled, then the aquaculture industry can contribute to occurrence of environmental problems.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Environmental ; Survey ; Shrimp ; Aquaculture ; Phosphorus ; Ammonia ; Chlorophyll a ; pH ; Salinity ; White spot disease ; Sampling ; Pollution ; Coastal waters
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 73pp.
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  • 18
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    A study on environmental pollutants (pesticides, heavy metals & detergents) in surface water, underground water and wastewater of Mangol Dam basin (Mazandaran province) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: At the present study, the environmental pollutants such as organochlorine pesticides (OCPs), heavy metals (during three seasons) and surfactants (during four seasons) were done 2009 to 2010 that located in Mangol Dam with longitude and latitude 52° 22' 49" N and 43° 15' 43" E, respectively. The aims of this study were to determine the seasonal pollutants matters in water samples of under-groundwater and surface water in 12 stations and the results are as follow: The maximum residues fluctuations of OCPs were determined in between all water samples such as aldrin and endrin about 1.51 and 2.85 µg/l, respectively. The mean concentration of aldrin and endrin about 0.66 ± 0.59 and 0.71 ± 1.07 µg/l, respectively were determined. The maximum seasonal concentration of OCPs were detected in fall, winter and spring for such as endosulfan sulfate, γ-BHC, heptachlor, endrin aldehyde compounds about 2.85, 0.34, 0.14 and 0.14µg/l, respectively. The maximum seasonal percentage range of OCPs were detected in fall, winter and spring in water samples such as endosulfan sulfate, DDD and β-BHC were 42, 25 and 25 %, respectively. The maximum seasonal concentrations of Zn and Fe elements were detected in spring about 1.12 and 8.22 µg/l, respectively. Also for Hg in spring was determined about 17.2 mg/l. The mean concentration of Zn, Fe and Hg in water sampled were 0.23 ± 1.01 and 1.21 ± 2.00 µg/l, respectively and 4.65± 6.38 mg/l. The maximum seasonal percentage range of heavy metals were detected in fall, winter and spring in water samples such as Ni, Zn and Fe were 66, 83 and72%, respectively. The maximum seasonal concentrations of heavy metal were detected in Sorkhroud and Polechelave stations during fall, winter and spring for only Fe about 1.48, 3.3 and 8.22 µg/l, respectively. The maximum concentration of linear alkyl benzene sulfonate (LAS) in last month of summer was 971µg/l. The mean concentration of surfactant during fall, winter , spring and summer was 418.62 ± 52.16 µg/l. The maximum seasonal percentage range of surfactant uring fall, winter, spring and summer in all stations of sampling were 76, 76, 100 and 100 %, respectively.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Organochlorine Pesticides ; OCPs ; Heavy Metals ; Surfactants ; Environmental Pollution ; Suface water ; Underground water ; Wastewater ; Samples ; Sampling
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 64pp.
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  • 19
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    Study of Metapenaeus affinis in coastal waters of Khouzestan province (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Study of M. affinis was carried out from June 2001 to December 2002 in two sampling areas of Khouzestan coastal waters. In this period, 18 cruises in Life- Buseif (western coastal area) and 13 cruises in Bahrekan (eastern coastal area) were came by using shrimp trawl net. In Life- Buseif fishing area, the CPUE of total shrimp was the highest (27.8 kg/ h) in September 2001. This parameter estimated 17.4 kg/ h for M.affinis in March. In this area, the highest biomass of total shrimps was In April 2002 (533.3 t). This value was 380.7! for M. Affinis on March. Maximum mean length of males and females of M.affinis were observed m January (13.4 & 11.5 cm respectively). Maximum and minimum sex ratio (f: m) of this species was 2.25: I July and 0.86: I in October 2002. Relative frequency of shrimp in total catch was observed m March (52.5%) in Life- Buseif. This parameter was 27.3% in February in Bahrekan area. In Bahrekan fishing area, total shrimp CPUE was highest (7.5 kg/ h) in March, and max. CPUE for M. affinis was 6.9 kgffl in same month. Maxmum biomass of total shrimp and M. affinis were 1800.4 t and t in March Maximum length of male and female of M.qffinis were 124 and 11.3 cm in March Maximum and minimum sex ratio (f: m) of M. affinis was 3.51 in May and 09: 1 in January, March and August 2002 respectively. According to the results of this study, shrimp catch season was opened in Life- Buseif from 3rd Nov until 29th Dec 2001 and from 6th Nov until 15th Dec 2002 winter shrimp catch season has been closed in Bahrekan since 2001.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Metapenaeus affinis ; Shrimp ; CPUE
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 58pp.
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  • 20
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    Comprehensive study on assessment of Sturgeons population genetic structure in the Caspian Sea (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The population genetic structure of five Caspian Sea sturgeon species was investigated. Totally 1121 samples of caudal and unault's fin tissue of the sturgeons (Acipenser persicus, A. gueldenstaedtii, A. stellatus, A. nudiventris and Huso huso) were collected from the Volga River (Russia), Ural River (Kazakhstan), Kura River (Azerbaijan), Sepidrud River and the coastline of the south Caspian in the Iranian waters as well as from the sampling stations selected for the marine survey for sturgeon stock assessment in the Caspian Sea. All samples were stored in 96% ethyl alcohol and transferred to the genetic laboratory of the International Sturgeon Research Institute. Genomic DNA was extracted using phenol-chloroform method. The quality and quantity of DNA was assessed by Agarose gel (1%) electrophoresis and spectrophotometry. The population genetic structure of Ship and Persian sturgeon was studied using both PCR-RFLP (D-loop and ND5/6 gene) and microsatellite technique and that of H. huso, A. stellatus and A. persicus were studied using microsatellite technique. After amplification of genes using PCR, the RFLP technique was used to digest mtDNA using restriction enzyme. The PCR products were electrophoresed on 6% sequencing polyacrylamide gels followed by silver nitrate staining. Data for PCR-RFLP were analyzed using REAP program and those from microsatellite technique were analyzed using Gene Alex. Population genetic parameters including allele frequency, expected and observed heterozygosity, effective allele, Shannon's index were determined. Genetic identity and distance were calculated following Nei criteria and Hardy Weinberg equilibrium was tested based on X2 and analysis of molecular variance (AMOVA) using Reap and Gen Alex at 99% confidence limit. Phylogenetic relationship was determined and drawn using TFPGA program. The population genetic structure and genetic diversity of the 1121 sturgeon specimens were determined. Three independent populations were identified for Acipenser persicus (two populations in the south Caspian in the Iranian waters and one in the north Caspian). Three independent populations were identified for A. gueldenstaedtii (Volga, Ural and South Caspian populations) using the microsatellite technique. Population genetic structure using PCR-RFLP revealed no genetic differentiation among the A. gueldenstaedtii specimens studied from the different regions using ND5/6 gene, while two populations (Ural and south Caspian populations) were detected for this species with the same technique using D-loop genes. Four independent populations (Volga, Ural, Kura and Sepidrud populations) were reported for A. stellatus using the microsatellite technique and four more populations which most probably belong to the autumn and spring races of the above mentioned independent populations were identified for this species. The present study also identified two populations for H. huso; The North Caspian population (in Volga and Ural Rivers) and The South Caspian population (in Golestan and Guilan regions) which were significantly different from each other (P〈0.01). The genetic population structure of A. nudiventris was studied using the microsatellite and PCR-RFLP techniques which revealed two populations for this species one in the Ural River and the other in the Sepidrud River (South Caspian). Comparison of the ND5/6 and D-loop genes studies in Russian sturgeon revealed that the D-loop gene is better than the ND5/6 genes in population's differentiation and is therefore strongly recommended for population genetic studies on sturgeons in the Caspian Sea. Genetic diversity studied using microsatellite technique was higher and more accurate as compared to that using RFLP. Nevertheless the RFLP technique was able to introduce molecular markers for the population’s species pacific identification. On developing suitable primers these studies can be speeded up and the cost of such studies can be cut down. However the drawback in using microsatellite technique for population genetic studies is that it cannot introduce a molecular marker for the identification of populations. The present study was able to introduce molecular markers to differentiate the ship sturgeon population in the south Caspian from that in the Ural River using the PCR-RFLP technique Based on the results obtained it is strongly recommended that all activities related to restocking and rehabilitation of sturgeon stocks in Iran be conducted on the basis of genetic principles. Also serious and immediate measures should be taken for the restoration and conservation of rare population of native species of Iran particularly in the Sepidrud region using genetic markers before they are become extinct.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Assessment ; Sturgeons ; Population ; Genetic ; PCR-RFLP ; Microsatellite ; Species ; Samples ; Tissue ; Acipenser persicus ; A. gueldenstaedtii ; A. stellatus ; A. nudiventris ; Huso huso ; Survey
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 329pp.
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  • 21
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    Preparation of inactivated vaccine to induce resistance against white spot syndrome virus by nuclear & non nuclear techniques (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The inactivated of white spot syndrome virus (WSSV) by Gama and Electron radiation and chemical material such as Formalin with antigen property have been done during last year. At the first time the stock of virus prepared and purred it. Six hundred of shrimp Fenneropenaus indicus with average weight 7 to 12 gram collected from shrimp research station in Heleh area and transported to Shrimp Research Institute in Boushehr province. Vibrio parahemolyticus obtained from Veterinary University of Tehran and then lyophilized it. The bacterial V. parahemolyticus inactivated with Gamma, electron and formalin and then injected to shrimp for activated the immune system. The source of viruses was identified through PCR, TEM and histopathology methods from the shrimp infected in 2009 occurrence of boushehr province. The virus injected to crayfish and collected the hemlymph for prepared the stock of WSSV virus. The virus was lyophilized and then exposed to gamma and electron radiation and formalin with optimal dose for inactivated the virus. The shrimp divided to 25 groups and each group consist of six shrimp and the vaccine exposed to shrimp with injected and bathroom methods and the documented the result after 15 days. In vivo virus titration was performed in Penaeus indicus. Inactivation of WSSV was carried out by a gamma cell instrument Nordian, model 220 with dose rate: 4.8 Gy/sec and activity: 20469 Ci. The LD50 of live virus stock was calculated 10 5.4 / ml and the optimum dose of gamma radiation beam to inactivate WSSV was obtained 14-15 kGy. The LD50 of live virus stock was calculated 10 5.4 / ml and the optimum dose of electron beam to inactivate WSSV was obtained 12-13 kGy and and the virus inactivated by formalin 0.5% V/V during 10 minutes.The LD50 of live virus stock was calculated by Karber method 10 3.29 /ml and 10 5.35 /ml, respectively. The V. parahemolyticus was inactivated with 8 KG. The result showed all vaccine during 2 hours have a good effect to shrimp viruses with the102.4 LD50 / 50 l titre. The result showed if the Gamma and electron vaccine increase to feed of shrimp the effect of vaccine is better than when used without feed. The end of experiment our study showed that the Gamma vaccine has the better effect to control WSSV during 2 hours with comparing the others.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Vaccine ; White Spot Syndrome Virus ; Gamma irradiation ; Electron Radiation ; Formalin ; Penaeus indicus ; WSSV ; Fenneropenaus indicus ; Shrimp ; V. parahemolyticus ; Preparation
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 148pp.
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  • 22
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    Investigstion on Effect prepared vaccine by Y-ray on production rate and resistance to white spot disease in Shrimp Litopenaeus vannamei (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: White Spot Disease (WSD) is a important disease due to economic impacts in shrimp industries. Spreading of this disease in shrimp farms can caused a 100% mortality during 3-10 days. Therefore control of this disease is a strategy in shrimp industry. Vaccination is a way to control of WSD. In several years ago during a project several type vaccine of this virus by association of Atomic Energy Organization is produced that among of them the virus inactivated by GAMA radiation had better results in laboratory. To test of this vaccine in field, a research pilot was carried out. Initially 20000 shrimp napliies were obtained from one of commercial hatchery in Bushehr province, then devided to two groups vaccinated and unvaccinated. The vaccinated group also devided . 26and PL12 , group B vaccinated at PL15and PL 5to two groups, group A that vaccinated at postlarva e (PL) Rrsults showed that difference of growth performance and survival rate between vaccinated and unvaccinated without challenge with WSV after 80 days is not significantly (P〈0.05). but survival rate in vaccinated groups after challenge with WSV was significantly (P〈0.05) further the unvaccinated group. Also the results showed survival rate of group B after challenge with WSV is better than group A and this difference was significantly (P〈0.05). this study showed vaccination of shrimp postlarvae with GAMA radiation vaccine can control of shrimp mortality in incidence of WSD in farms.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Litopenaeus vannamei ; WSSV ; Gamma radiation ; Vaccination ; Y-ray ; White spot disease ; Shrimp ; Nauplii ; Growth ; Survival rate ; Mortality
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 65pp.
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  • 23
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    Study on inheritance of microsatellite markers in hybrid of chalcaburnus chalcoides and vimba vimba persa in southern part of Caspian Sea (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: In order to study the Inheritance of Microsatellite Markers in the hybrid of Chalcalburnus chalcoides and Vimba vimba persa in the Southern part of the Caspian Sea, hybridization between the species was carried out during spring (2009) in Shahid Ansari reproduction and culture workstation. 30 samples from the produced larva and also small piece of dorsal and pectoral fin of their parents were collected and stored in pure ethanol (96%) and transferred to the biotechnology of the Caspian Ecology Academy. DNA was extracted from fin samples of the parents, whilst the whole F1 progeny was used to isolate the DNA by Phenol-chloroform method. Quantity and quality of extracted DNA was determined using 1% Agarose electrophorus. DNA was used for PCR ampilification of 10 microsatellite loci. The amplifications were run on 6% Polyacrylamid electrophorus. The present study showed that 7 out of 10 markers were polymorphic and variated between the parents. Goodness of fit to the Mendelian inheritance ratio was determined by X2 analysis. 4 primers were segregrated according to Mendelian expectations at a significance level of P 0.05. Based on UPGMA dendrogram (Nei, 1978) two different clusters were obtained in which Chalcalburnus chalcoides and hybrid were in the same cluster but Vimba vimba persa was in the other. It seems that Vimba vimba persa is a joint ancestor for these two. High heterozygosity presence in parents and their F1 shows the high potential of genetic variety in Chalcalburnus chalcoides and Vimba vimba persa which from the aquaculture management and conservation view is important and considering the high genetic variety in their hybrid, the hybrid has the sufficient potential for race improvement.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Ecology ; Chalcalburnus chalcoides ; Vimba vimba persa ; Hybrid ; Microsatellite ; Hybridization ; Culture ; Samples ; Larvae ; DNA ; Inheritance ratio ; Heterozygosity
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 85pp.
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  • 24
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    Epidemiological study on some environmental and management parameters affecting on WSD occurrence in Fenneropenaeus indicus and Penaeus vannamei (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: For the first time white spot disease (WSD) was reported in shrimp farms of khoozestan province, in southwest of IRAN in 2002. Then in 2005 the neighbor province, boushehr, was contaminated. In 2008 WSD outbreak reported in sistan-bloochestan province in southeast of Iran. In 2015 all of southern shrimp farms of country except Hormozgan, the middle southern province, which has remained free of WSD, are being contaminated. White Spot disease suspended shrimp culture in thousands hectares of shrimp farms. Considering that white spot disease has not been observed in Hormozgan province yet, the question is; to what extent environmental and management factors participated in preventing WSD outbreak or cause WSD outbreak. In this study (20102012), the effects of environmental factors and management, stressors that decrease immune system function of shrimp are discussed. In addition, the role of pathogen as the main factor of outbreak is discussed. The goal of this study is to define environmental parameters and management practices associates with outbreak of white spot disease in affected provinces and discover reasons of being Hormozgan province free of this disease. In this study the role of the local environmental factors and management practice stressors in susceptibility to WSD was determine. Both the effects of environmental factors in water of ponds including total ammonia, nitrogen, dissolved oxygen, pH, salinity, transparency, and temperature and management issues related to biosecurity are studied. There were overlaps on physical and chemical parameter values obtained in clear areas with contaminated areas .Results of the data analysis suggest that lack of association with WSD incidence was 7 times greater than WSD incidence despite of disease outbreak in sistan-bloochestan province, so other sources of white spot disease virus incidence was suspected in affected areas. Histopathological examinations and polymerase chain reaction (PCR) tests during project performance did not reveal white spot disease virus evidences in post larvae examined from khoozestan province stocked in farms but disease outbreak was happened in that farms , so we suspected to management practice include feed , pond preparation and carrier of disease . Recorded values of temperature and salinity in some months during inspection in Hormozgan province specified stressful condition that may lead to WSD outbreak, however the disease did not appear. Therefore the hypothesis that the water physical and chemical conditions are reasons to prevent disease outbreak in Hormozgan province is being rejected. The policy of Hormozgan’s fishery authorities, to replaced Fenneropenaeus indicus with specific pathogen free Litopenaeus vannamei, that is more resistant to some of diseases, before incidence of WSD in farms and to before being endemic in the Hormozgan province, made an advantage compare to affected southern provinces that introduced Litopenaeus vannamei after WSD prevalence to their farms. However it does not guarantee to maintain current trend of being Hormozgan province farms free of white spot disease. Therefore establishing the principals of biosecurity are strongly emphasized. Strategies taken by the proficient authorities in preparation of SPF shrimp broodstock can be the most important factor in preventing WSD. Regarding biosecurity principals purchased feed must be free of shrimp head powder. Construction the new shrimp farms should be as far as it could be away from contaminated areas.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Environmental ; Physical ; Chemical ; Epidemiological study ; WSD ; Fenneropenaeus indicus ; Penaeus vannamei ; Shrimp ; White Spot Disease ; Temperature ; Salinity ; PCR ; Polymerase Chain Reaction ; Ammonia ; Nitrogen ; Dissolved oxygen ; pH ; Transparency
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 468pp.
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  • 25
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    Assessment of different methods of water treatment on decreasing pathogenic bacteria in shrimp hatcheries (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The project of "Assessment of different methods of water treatment on decreasing pathogenic bacteria in shrimp hatcheries" achieved by mean of reviewing the effectiveness of various methods on the water treatment to reduce bacterial load of sea water. The project performed in Bandargah region of Bushehr province in order to checking the effect of different methods on the sea water and bacterial population. This project has been done over a year. In this study a total of 138 samples was collected during seven times and was evaluated as well as 7 treatments. In this study, the comparison of the effect of each factors (sand filter, chlorine, ultra violet ray, chlorine combined with ultra violet ray) was analyzed on the sea water by means of disinfection. During this study, other environmental factors such as water temperature, dissolved oxygen, pH and salinity was measured till we can ensure that they don’t have any negative impact on the treatments. Results showed that the density of total bacteria in sea water (Control) was averagely 5187 CFU/ml, this was done while the total density of bacteria in the sea water, reservation pool sample, sand filter water, water that irradiated with ultraviolet rays, chlorinated water and the water that was influenced by both chlorine and ultra violet ray was respectively 5187 2137, 4449 2042, 2782 1335, 1164 692, 143 104, 87 76 CFU/ml. Therefore, reservation pool, sand filter, ultra violet ray, chlorine, chlorine-ultra violet ray each of them was reduce respectively 14, 46, 77, 97, 98 percent of the density of total bacteria or in other words they reduced 0.06, 0.27, 0.65, 1.56, 1.77 log of the total population of bacteria in water. Moreover, the results from the total count of vibrios showed that reservation pool, sand filter, ultra violet ray, chlorine, chlorine-ultra violet ray each of them was reduce respectively 17, 47, 74, 98, 99 percent of the density of total vibrios or in other words they reduced respectively 0.08, 0.27, 0.59, 1.66, and 1.95 log of the total population of vibrios in sea water. Also the bacteria that isolated from each treatment were identified with the use of biochemical methods that totally were includes a wide range of bacteria, the identified vibrios were: Vibrio alginolyticus, V. costicola, V. fischeri, V. fluvialis I, V. fluvialis II, V. harveyi, V. natriegens, V. nigripulchritudo, V. parahaemolyticus, V. plagius I, V. vulnificus, V. anguilarum I, V. campbellii, V. nereis, V. proteolyticus, V. splendidus, and Unknown V. spp. Other identified bacteria belonging to the following genus: Acinetobacter, Aeromonas, Alcaligenes, Bacillus, Borkholderia, Eschricia, Enteroacter, Flavobacterium, Micococcus, Proteus, Pseudomonas, Serratia, Staphaphylococcus, Streptococcus, and Unknown Bacteria spp. By comparing the results, we concluded that the best result was related to treatment that the sea water had been disinfected after the combined effects of chlorine and ultraviolet ray. It was determined that this process in compared with other treatments, had been reduced the total number of bacteria, the total number of Vibrios, the number of sucrose fermentative vibrios, and the number of non-sucrose fermentative vibrios respectively 98, 99, 98, 99 percent. In other words, the number of bacteria was reduced respectively 1.77, 1.94, 1.82, 2.23 log. So in this study, the applied method was introduced as the most effective way of disinfecting sea water. Meanwhile, none of pathogenic bacteria for shrimp including Vibrio harveyi was isolated by using this treatment. Comparing the results showed that the combined effects of chlorine and ultraviolet radiation is the most effective applied method for disinfecting sea water.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Shrimp ; Water treatment ; Sea water ; Bacteria ; Hatchery ; Assessment ; Population ; Temperature ; Dissolved oxygen ; pH ; Salinity
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 84pp.
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  • 26
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    Molecular identification of bacterial and fungal pathogens in certain disease-free shrimp (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: At present, the aquaculture industry to provide proper instructions in the field of health management, including production of Specific Pathogen Free shrimp (SPF), require sensitive and reliable methods for the detection and identification of pathogenic microorganisms. Molecular methods which used in the detection of microorganisms have a high discriminatory power in the taxonomy and in relation to libraries in the world. On the other hand, the accurate identification of microorganisms, providing the genetic data bank of shrimp pathogens and maintenance of these strains is the step to promote further research on the mechanisms of pathogenesis of pathogens, diagnosis, treatment, prevention of disease, identify indigenous production kits, diagnosis re emerging and emerging diseases and their origin. Therefore, in this project, by using ribotyping technique, native isolated pathogenic bacteria and fungi were identified and recorded in the gene bank database center. During sampling of shrimp and water of Specific Pathogen Free shrimp center, 40 bacterial strains were isolated, which 8 of them had the most frequency and identification based on 16S rDNA sequencing was performed. Bacteria identified are: Vibrio nigripulchritudo strain IS013(GenBank:KP843725), Vibrio brasiliensis strain IS014 (GenBank:KR186076), Vibrio rotiferianus strain IS015 (GenBank:KR186077), Vibrio azureus strain IS012 (GenBank:KJ018724.1), Vibrio owensii strain IS016 (GenBank:KR186078), Agarivorans gilvus strain IS017 (GenBank:KR186079), Vibrio brasiliensis IS018 (GenBank:KR186080) and Vibrio alginolyticus strain IS019 (GenBank:1817854), which were recorded in The World Bank genes. In this study fungal isolates were not detected.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Bacteria ; Fungi ; Ribotyping ; 16S rDNA ; Shrimp ; Specific pathogen free ; Vibrio rotiferianus ; Vibrio owensii ; Vibrio brasiliensis ; Vibrio azureus ; Agarivorans gilvus ; Vibrio alginolyticus ; GenBank
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 64pp.
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  • 27
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    Study of some biological characteristics of golden grey mullet (Liza aurata) in Iranian Coastal waters of the Caspian Sea (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: During last 65 years the catch of mullets had increasing trends with some fluctuations in the Iranian coastal water of the Caspian Sea .In this period about 138 thousand tons of mullets have been caught. Mullet’s account for 35% of total catch annually .In recent years species composition of mullets has chanched in the Iranian coastal water of the Caspian Sea and catch composition of golden grey mullet increased from 76% in 1995 to 98% in 2006. In this survey some biological characteristics of golden grey mullet have been studied in Iranian coastal water of the Caspian Sea .Fish samples have been gathered from commercial catch of beach seine cooperatives monthly in Iranian coastal water of the Caspian Sea over 2006 and 2007. In the laboratory fishes have been measured biometrically and biological parameters have been calculated .Also catch statistics of mullets during 2006-2007 have been obtained and discussed. Results showed that the catch of mullets in beach seine cooperatives during 2006 and 2007 was 4181 and 3685 tons respectively that golden grey mullet contribute 99% and 98% of the catch composition of mullets respectively. Length range of golden grey mullet caught by beach seine cooperatives was 19-50.2 cm with mean length of 32.7 ± 6.4 (± SD) and weight range was 67-1475 gr with mean weight of 411 ± 255 gr. The age structure of this species was comprised 2-10 years old fish with mean age of 4.42 years old. In this survey totally the sex ratio of male:female of golden grey mullet was 356: 434 that was significant variation from equal sex ratio. Pick of the spawning in Guilan province was in October and in Mazandaran and Golestan provinces was in November. In October the proportion of spawning females declined from western area (Guilan province) towards eastern parts (Golestan province).The highest proportion of spawning females was in December in Golestan province. The highest GSI index value was observed in September and October and it was decreased in November and December and it was consistent during January till April. The mean absolute fecundity was 700881 ± 429987 eggs with minimum and maximum fecundity of 200112 and 2282862 eggs respectively. The Lm 50% for female golden grey mullet was calculated as 33.6 cm.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biological ; Commercial ; Golden grey mullet ; Liza aurata ; Species ; Survey ; Samples ; Male ; Female ; Sex ratio ; Spawning ; GSI ; Fecundity ; Coastal waters
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 56pp.
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  • 28
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    A study on the shrimp Nursery grounds in Bushehr waters (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Study of shrimp nursery ground in the Northern part of Bushehr province from Farakeh creek to Genaveh The fourth phase of "study of shrimp nursery ground in the Northern part of Bushehr province" was carried from October 2000 to August 2001. Sampling was carried for larval stages by using Bongo net with 500 micrometer mesh size, and for adults with Trawl net of I centimeter mesh size. In 12 stations, 25576 juvenile and adult shrimp and 651.13 larves were collected and identified. Most of these samples belonged to the families of Sergestidae, Penaeidae Caridea. Acetes cfsergestidae family, both in adults and larval stage (57% for adult and 84% for larval stage) were identified. Adults wrere caught only in Ramleh creek in Feb. 2001, and the most presence of Acetes' larvae were also in Ramleh creek in Dec. 2000. 33% of adults were belonged to Metapenaus stebbingii of Penaeidae family. The most presence of this species was in Feb. and Mar. 2001. Although no larvae of this species was found in the field, it seems there is no significance between pH, salinity and area temperature with the presence of these species of shrimp.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Shrimp ; Nursery ; Larval stage ; Sergestidae ; Penaeidae ; Caridea
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 66pp.
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  • 29
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    Investigation of biology of tiger shrimp in different depths of the Persian Gulf (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Investigation ; Biology ; Tiger shrimp ; Shrimp ; Depth ; Penaeus monodon
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 47pp.
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  • 30
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    A survey on effects of shrimp aquaculture on the coastal waters of Bushehr (Helleh region) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This survey was designed in the Helleh region, to understand effects of aquaculture industry on coastal waters of Busher, in 2000. Sediment and water samples were collected from effluent canal, influent canal and sea during the 7 month period (harvest and post harvest season). The variations of selected water quality parameters were controlled monthly. Results show that, in effluent canal, estimated parameters of water was higher than other stations every month, and tend to decreased after being discharged into the sea and got closed to normal level. Comparison of above data with permit able range of municipal and aquaculture waste and comparison of present data with data of normal condition of region, show that, aquaculture industry of Helleh region has not had any obvious negative effect on the coastal waters of Bushehr, in the culture year of 2000.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Survey ; Aquaculture ; Sediment ; Samples ; Culture ; Coastal waters ; Shrimp ; Parameters ; Water quality ; Comparison
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 65pp.
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  • 31
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    The Study of nutrition and feeding position of shrimp, cultured in IRAN (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The culture of Iranian Shrimps began in Bushehr in 1372. Early the green or the pink tiger shrimp (Penaeus semisulcatus) which were found in most habitats of the Persian Gulf and Oman Sea were considered in breeding and reproduction, but due to reproduction difficulties, it was rather replaced by the Monodun imported species, then the production of the white shrimp Metapenaeus affinis and Then P.merguensis in some southern areas of the country such as Hormozgan has began in small scale,but none of these species couldn't meet the economical needs of the consumer society and they were not indelible and dominant enough in the shrimp aquaculture industry of the country until breeding of Indian White Shrimp (Fenneropenaeus indicus) has began in large scale in the provinces of Khuzestan and Bushehr, which are seen as the heart of shrimps reproduction and breeding, and in Hormozgan and Sistan. the incidence of white spot viral syndrome (White Spot Syndrom Virus) led to the import of the western white leg shrimp from the USA (P.vannameii) by the Iranian Fisheries Research Organization (IFRO) in 1383, at the present all southern and northern breeding farms of the country (fundamental measures has been carried out in line of shrimp reproduction in Gorgan province in 1386) has put the breeding of this shrimp species as the single breeding species at top of their agenda. The subject of feeding the breeding shrimps is widespread and regarding to the non-proprietary species there are still some species which contain high protein than the other species in studying the different shrimp species requirements. In Iran due to the scarce factories producing the shrimp's food, the alimentary compound of the breeding shrimps during industrializing years of this type of breeding even after the non-aboriginal western white leg shrimp species being inclusive was stable and through different biological processes including: Naplies which feeds from its yalk and by entry into zoa stage start eating only small-sized phytoplankton. And by entry into the stage between zoa and mysis it feeds from phyto and zooplankton simultaneously and entering the post-larva stage it stars sarcophagi. and after 15 post-larva stage entering the growth and transition stage to earthen ponds feeding by concentrated industrial foods in large and small packages depending on young and adult mouth and due to feeding requirements of every stage the alimentary compound is nearly as follows: Digestible protein, energy, fat, carbohydrates, vitamins and minerals will start and the final product will be released in market Or they will be used in the later years of laying eggs and feeding for brood stock. In feeding section the details of every stage and their food's nutritional needs at each stage and will be fully described.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Economical ; Shrimp ; Food components ; Biological processes ; Nutrition ; Feeding ; Culture ; Tiger shrimp ; Penaeus Semisulcatus ; Breeding ; White shrimp ; Metapenaeus affinis ; Aquaculture ; White Spot Syndrome Virus ; Phytoplankton ; Zooplankton
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 178pp.
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  • 32
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    Improving over ripe Caviar through osmotic and physical methods (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Every year more amount caviar is converted into pressed caviar due passing of complative processes from 4 stage to 5 stage, and according to carried out of countits amount was over up L5 ton in sturgeon fish hatchery (Asetra and Sevroga caviar). The purpose of this project was the preventing of caviar converting into pressed caviar and for this order established doing operations of this project in two main primmary stages. In primmary stage, this caviar divided to injected and uninfected caviar, that in this way the processing of both kinds caviar did by 11 formulas. And all processed samples store in chill room in -3 degree centigrade cold and carried out the quality experiments (microbial, chemical, and organoleptical experimcnts) of samples in 6 month and then results evaluation 4 choosed for Asetra caviar and 3 form Was for sevroga caviar and started the main stage of operation in 2 seasons. For processing Asetra caviar used from below formula: 1 - water and salt 5 and 20 percent. 2 water and salt 5 percent and dry dure salt (60 gr). 3 - Water and salt 5 percent and dry mixture salt (56 gr). 4 - Processing of caviar by pure dry salt (60 gr) and mixture sait (56 gr) and forsevroga caviar used from formulas: 1 - water and salt 5 percent and pure dry salt (44 gr)e 2 - water and salt. and mixture dry salt (46 3 -The processing of caviar by pure dry salt (47 gr) and mixture (46 gr). In results of qua}ity evahlation of samples in main stage for 6 months showed that the best formula in case Asetra caviar (injected and uninjected) was and 1 formula respectively and the comparing investigation between Asetra caviar (injected and uninjected) showed that the best processed samples were in I formula and also the best formula in case sevroga caviar (injected and uninjected) was 1 formula. The comparing investigation between sevroga caviar showed that the sample was 1 formula.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Caviar ; Osmotic and physical methods ; Sturgeon ; Samples ; Hatchery
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 62pp.
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  • 33
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    Development gene data bank of cultured shrimp species in Iran (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: DNA barcode is a short, standard well known sequence of cytochrome oxidase І gene. By using this DNA sequence can be realized that each animal, plant or fungus belongs to which species. in this research, samples were collected from imported cultured shrimp Litopenaeus vannamei and and 6 Persian Gulf and Oman Sea shrimp species which classified based on traditional systematically as: Penaeus semisulcatus, Fenneropenaeus merguiensis, Metapenaeus affinis, Parapenaeopsis Stylifera and Fenneropenaeus indicus. After examination of DNA barcode sequence, molecular and bioinformatics operations of each sequence in the Consortium for the Barcode of Life (CBOL) and National Center for Biotechnology Information (NCBI), phylogenetic analysis of each sample was determined and similarity of each sample with NCBI and CBOL database was checked and the closest species to each sample were specified. According to the results different samples of L. vannamei, . banded P.semisulcatus, F. merguiensis and F. indicus have more than 97% similarity to the same species of other countries. non banded P.semisulcatus had 80.07% similarity to banded P.semisulcatus, M. affinis samples had 90.3% similarity to Metapenaeus ensis and Parap. Stylifera had 93.44% similarity to Parapenaeopsis coromandelica in the CBOL. This funding confirmed the need for further investigation and possible announcement of new species.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: DNA barcode ; Shrimp ; Litopenaeus vannamei ; Penaeus semisulcatus ; Fenneropenaeus merguiensis ; Metapenaeus affinis ; Parapenaeopsis stylifera ; Fenneropenaeus indicus
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 72pp.
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  • 34
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    Environmental risk assessment of the activity of specific pathogen free shrimp production centers (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This study is a part of the national and technology master plan entitled "Attain to technical knowledge of specific pathogen free shrimp production and cut off to dependence on foreign products". The goals of this work were to assess and analysis the risk factors of production of specific pathogen free Litopenaeus vannamei shrimp in its all stages (broodstocking, laravl production, shrimp farming, feeding and water quality), determining the pattern of outcomes monitoring, management and control of outcomes from April 2012 to September 2015 in Bandargah research station and Persian Gulf SPF Shrimp research station and their environment, belonging to Shrimp Research Center, located in Bushehr. Identification and screening of risk factors have been done based on Environment Aspect and Effect Analysis method, (EA)2, using a fine statement questionnaire and quantification of risks. The number of identified risk factors in Bandargah research station was 15 while it was 13 in Persian Gulf SPF Shrimp research station. The more number of risk factors in Bandargah station in respect to its number in Persian Gulf station is might due to lower level of biosecurity, physical structure and aging of Bandargah station and existence of two possible source of contamination, the Bushehr nuclear power plant pollutants and Bandargah fishing harbor, near to the station. On the basis of results, the maximum values of APN in Bandargah station was 42 while it was 30 in Persian Gulf station. These results revealed that the level of risk in Bandargah station is lower than that in Persian Gulf station. The most probable risks in both studied research stations are related to transmission of pathogens in shrimp feeding and water intake pollution.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Environmental risk assessment ; Shrimp ; Litopenaeus vannamei ; Specific pathogen free
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 80pp.
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  • 35
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    Partial harvesting from intensive shrimp (penaeusvannamei) culture pond in helle site (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: In this investigation, were examined the effect of partial harvesting on optimal management of shrimp culture. In order to achieve to the purpose 6Pool0.4/hectare was selected. Three pool with density Twenty-five per sq. m as a witness and another three pond by density fifty per sq. m as the test sample were storage. Based on the results of biometry and biomass estimates of pool was calculated and provided amounts daily meals for shrimps. The first harvest took place after a period of120 days from the test pools .Average of harvest from pools B4, B6andB7 ,was determined respectively, 1373,1365 and1105kg.Second harvest from treatment poolsB4, B6andB7were determined, respectively, 1663,1748 and1783kg. Final harvests from the pools were after 150 day. Harvest average from control pools B1, B2 and B3 was calculated and estimated respectively, 1824, 2000 and 1685 kg. The Average of FCR was calculated 1.51 in control samples. In general, the average of feed conversion rate of the treatment samples were calculated 1.51.We did not see the difference between FCR of control and test samples. According to the project's achievements, we can say, partial harvesting has an important role to increased productivity of shrimp farms in Hellh site from BushehrProvince.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Partial Harvesting ; Shrimp Culture ; Feed Conversion Rate ; Penaeusvannamei ; Culture Pond ; Biomass ; Samples
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 30pp.
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  • 36
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    Survey on White Spot Virus infection in wild shrimps and crabs in Bushehr province costal waters (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: After severe mortality due to white spot disease incidence in cultured shrimps of Helleh grows out farms, disease spread to Delvar,Mond, Boeyrat and Bandar Rig sites in Bushehr province during 2005. Many crustaceans keep this virus in the body, act as a carrier, and can spread of the infection. In order to investigate status of contamination of wild shrimp and crab's population to white spot virus in Bushehr province waters (north of Persian Gulf), samples were collected and tested by Nested PCR for detection of WSV from estuaries and input and output channels water around the Helleh, Shif and Mond sites during 2007 and 2008. Total tested shrimps were 401 peaces, includes green tiger shrimp (Penaeus semisulcatus) 220 pieces, white shrimp (Metapenaeus affinis) 181 pieces, also total tested crabs were 292 pieces includes Ocypode rotundata 68 pieces and Portunus pelagicus 224 pieces. All the samples showed negative results of WSV contamination. However, it can never be sure that the population is free from WSV unless we collect a random sample of 300 pieces from each species and test them individually for WSV. Therefore, we will be confident up to 95% that the wild sources in that area have no prevalence of 1% or upper WSV contamination.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: White spot disease ; WSV ; Shrimp ; Crab ; Nested PCR ; Survey ; Coastal waters
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 55pp.
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  • 37
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    Survey of diversity, distribution, abundance and biomass of macrobenthic fauna in the southern Caspian Sea (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Sediments samples were collected using Veen Grab Sampler (0.1 square meter) at 8 transects namely Astara, Anzali, Sefidroud, Tonekabon, Noshahr, Babolsar, Amirabad, Torkman in the southern of Caspian Sea. Five stations were selected at 5, 10, 20, 50 and 100 meter depths in each transect. Sediments were sampled triplicate at each station. Samples also were collected during four seasons (spring (May), summer (July), fall (November) and winter (January)) in 2009. Results of this study showed that species composition of Macrobenthos consisted of 32 species which belonged to 7 families of Polychaeta, Crustacea and Bivalvia at studied area. In addition, Oligochaeta identified in “Class”, Chironomidae considered in Insecta categories “Family” and Streblospio spp. (Polychaeta) was recognized in “Genus”. Gammaridae and Pseudocumidae of Crustacea with 12 and 10 species had the highest species diversity compared to other groups, respectively. Polychaeta was consisted 75.5 percent of total abundance of macrobenthos which the major abundance (equal 62.4% of total abundance) were belong to Streblospio spp. from Spionidae family, while its biomass was equals 5.11% of total macrobenthos. In contrast, Cerastoderma lamarcki species from Bivalvia Class with only 1.7% of total abundance of macrobenthos allocated 69 percent of total biomass. In the southern of Caspian Sea, average total abundance was significantly less at 4 western transects (Astara, Anzali, Sefidroud, Tonekabon) compared to 4 eastern transects (Noshahr, Babolsar, Amirabad, Torkman) (p〈0.05). The highest average abundance of macrobenthos (10655±1246SE ind/m2) was observed at transect of Torkman, and lowest value (4032 ± 686SE ind/m2) was recorded at transect of Sefidroud (p〈 0.05). Generally, minimum species diversity were obtained at 20 m depth in all transects and the maximum value was observed at 5 m depth in most of transects (p〈0.05). In contrary, maximum average abundance of Macrobenthoses was at 20 m depth in transects of Anzali, Sefidroud, Tonekabon, Nowshahr and Amirabad compared to other depths. Macrobenthoses abundance average in 5 m depth (except Astara and Torkman) was less than other depths in 6 transect (p 〈 0.05). Total average abundance and biomass of macrobenthos was 5976±583SE ind/m2 and 43.675 ± 11.402SE gr/ m2, respectively. Maximum and minimum of abundance of macrobenthos were observed in summer (7714±778 ind/m2) and winter (4071 ± 340 ind/m2), respectively. Maximum and minimum of biomass of macrobenthos were obtained in fall (50.271±13.258SE gr/ m2) and in summer (35.123 ± 8.903SE gr/ m2), respectively (p〈 0.05). Percent of total organic matter (TOM) were low in 5 and 10 m depths and increased toward offshore depths. TOM percent was 2.06±0.11SE at 10 m depth and increased to 4.62 ±0.17SE in 100 m depth. Percent of silt and clay (grains size less than 63 micron) had positive significantly correlation with percent of TOM (p〈0.01).While they had negative significantly correlation with percent of sand (grains size between 63 and 1000 micron) (p〈0.01). Percent of silt and clay like organic matter, had ascending trend toward to depth increased and varied from 44.4 ± 4.06SE percent in 5 m depth to 96.5 ± 0.59SE percent in 100 m depth. In contrast, percent of sand decreased toward depth and varied from 54.5 ± 4.13SE percent in 5 m depth to 2.8 ± 0.53SE percent in 100 m depth. Result of current study showed that total abundance of macrobenthoses had positive significantly correlation with TOM percent (p〈0.01) and silt/clay percent (p〈0.05). Abundance of Oligochaeta had positive significantly correlation (p〈 0.01) with TOM and silt/clay percent. Two groups of Polychaeta, Gammaridae and Cerastoderma lamarcki had negative significantly correlation with TOM and silt/clay percent (p〈 0.01), and every four aforementioned groups had positive significantly correlation with sand percent (p〈 0.01). Overall, different correlation between abundance of various macrobenthos groups and TOM percent and type of grain size of sediment could be related to fluctuation of abundance of various macrobenthos groups at difference transects and depths. On the other hand, in study area were occurred simultaneously some phenomena such as increased abundance of Oligochaeta and Polychaeta, dominance of Streblospio Genuse (Polychaeta group), and decreases abundance of Bivalvia and appearance of Menemiopsis leidyi which need to study more and monitoring of this area.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Macrobenthos ; Diversity ; Distribution ; Abundance ; Biomass ; Survey ; Fauna ; Sediments ; Samples ; Streblospio spp. ; Cerastoderma lamarcki ; Menemiopsis leidyi
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 105pp.
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  • 38
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    Evaluation of the immunity factors (THC, TPP, PO, SOD, POD) of shrimp fed with the yeast Saccharomyces cerevisiae compared to shrimp fed without yeast (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Effects of S. cerevisiae on immune parameters of the L. vannamei after 14 days of S .cerevisiae feeding were evaluated in this study. For this purpose a total of 300 pieces of shrimp with an average weight of 30 to 35 grams were selected from a pool shrimp Abadan CHOEBDEH. After making sure the health, absence of necrosis on the surface of the body, cuts antenna, shrimp were transferred to the center of BANDAR IMAM Research Station. Adaptation was carried out for 3-5 days. After the adaptation, shrimps were screened for virus (WSSV, TSV, MBV, HPV, YHV, BP, IHHNV and IMNV) and vibrio bacteria.After screening shrimps divided to two groups with three replication (including 50 pieces of shrimp in triplicate). The experimental diet has the commercial shrimp composition ,but 2 g of S. cerevisiae substituted 2 g of fish meal. Shrimp of first group (T1) for 14 days with food containing nutritional yeast and shrimp in second group (T2) were fed with normal diet without yeast. After 14 days Immune Factors and survival rates in both groups were evaluated. The results showed that the relative survival rate between the two groups showed no significant difference. But Immune Factors (THC, TPP, PO, POD and SOD) in the treatment fed yeast (T1) compared to control treatment (T2) showed a significant increase. In conclusion these results suggest that the increased survival rate and resistance of shrimp after S. cerevisiae consumption occurs through immune modifications, such as increases in THC, TPP, SOD, SOP and PO activity.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Evaluation ; Immunity factors ; THC ; TPP ; PO ; SOD ; POD ; Shrimp ; Saccharomyces cerevisiae ; Yeast
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 56pp.
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  • 39
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    The assessment quality of effluent from shrimp aquaculture site in Gwater (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Present study was conducted in shrimp farm located in east of Chabahar in Sistan and Balouchestan province from August 2001 until May 2003. Fourteen stations at three locations (i.e. supply channel, drainage channel and Gwatr Gulf) were selected to determine physical and chemical factors such as; temperature, salinity, dissolved oxygen, pH, nitrate, nitrite, ammonia, phosphate, silicate, total suspended solid and turbidity. Sampling was conducted twice a month during the shrimp culturing period and monthly at other times. Water samples were collected every two weeks to determine Chemical and Physical factors, Chlorophyll a, Phytoplankton and Zooplankton. Sediment samples were collected seasonally to examine Macrobenthos, Grain size and Total Organic Matter. The results revealed different bioenvironmental area in the three locations (i.e. supply channel, drainage channel and Gwatr gulf) over the study period. One-way analyze of variance of chemical and physical factors indicated significant difference between different times as well as different stations (p〈0.05). The negative relationship was observed between the culturing production area and the amount of average dissolved oxygen, pH and total suspend solid. An increase in the culturing production area in 2002 as compared to that of 2001 causes a decrease in the amount of these factors. However, it dose indicates positive relationship with nitrate, nitrite, ammonia, transparency and chlorophyll. Forty nine phytoplankton genus were identified, which mainly belong to diatoma, dinoflagellate and green blue algae. Diatom was dominated among these groups. Diatom and green-blue algae and diatom comprised %79, %69 and %64 of phytoplankton flora respectively in supply channel, Gwater Gulf and drainage channel. Copepod and thintinnida were the major groups of zooplankton. High abundance of bivalve, Polychaetes and amphipoda were found in supply channel, Gwatr Gulf and drainage channel respectively. Analysis of grain size showed the existence of sandy sediment in Gulf of Gwatr and sandy-loom in supply channel. Drainage channel sediment found to be sandy-loom, sandy-silty-loom and sand loom. Among three locations, the highest TOM was found in drainage channel. In last location, production area in 2002 increased as compared to that of 2001, which showed negative relationship with salinity, nitrate, nitrit, ammonia and chlorophyll a. Drainage of the Gwatr s shrimp farm did not cause any thermal contamination in the location. Shrimp aquaculture activities caused an increase in the average of salinity, range of pH in the Gwatr Gulf.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Physical ; Chemical ; Shrimp ; Aquaculture ; Temperature ; Salinity ; Dissolved oxygen ; pH ; Nitrate ; Ammonia ; Sampling ; Phytoplankton ; Zooplankton ; Sediment
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 153pp.
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  • 40
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    Evaluation of shrimp culturing and hatcheries sites in Hormozgan province (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This project was performed to evaluate the qualitative and quantitative operation of hatcheries and shrimp culture Sites in Hormozgan province during years 2004-2006. All the active hatcheries including one hatchery located in Bandar-e-Jask, Three hatcheries in Bandar-e-Kohestak, one hatchery in Bandar-e-kolahi and two hatcheries in Qeshm island also three shrimp culture sites including Tiab-e-Shomali, Tiab-e-Jonubi and sayeh khosh were surveyed. The Information about scientific and technical persons, employments, productions, important problems and also situation of costs and net were recorded in special forms. Results were analyzed by descriptive statistics. Results revealed that about 50% of the hatcheries located in Hormozgan province are closed through the different problems such as, failure of customer, failure of a distinct plan for post larvae producing, high coast of producing parameters and so on. Results also revealed that only 7.1% of farms located in sayeh khosh area, about 40.2 % of farms located in Tiab-e-Shomali and 62 % of farms located in Tiab-e-Jonubi areas are active. The two later areas have established about ten years ago. The most problems of most farm in Hormozgan province are the failure of sufficient knowledge of farms managers, high cost and low quality of shrimp foods manufactured by huge factories and low cost of cultured shrimps. Final, it can be said that problems of hatcheries are depended to shrimp farms problems. If problems of farms be did, problems of hatcheries will be did.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Shrimp ; Culture ; Sites ; Hatcheries ; Farms
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 102pp.
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  • 41
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    Determination of sustainable exploitation level of Penaeus semisulcatus in Bushehr province water (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This study was carried out in Bushehr province waters (49ْ 40E and 30ْ 00N to 52ْ 00E and 27ْ 40N) in July and August 2010-2011. The objectives of this study were to determine the opening and closure periods of shrimp and to estimate of the shrimp biomass in the study area. Based on the total length, the opening season of shrimp fishery in 2010 and 2011 were proposed on 12 and 22rd of July, respectively. The closed season was determined based on the remaining of 20% of shrimp stock and proposed on 12 and 29th of August, respectively. The primary estimation of the biomass in these years was 840 and 455 metric tones, respectively. While the total catch were estimated 847 and 859 tones in these years. During this study 9 species of the penaeid and solenoserid species were identified. The percentage of the green tiger prawn to the other species in the study years were 73% and 92%, respectively.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Green tiger prawn ; Penaeus semisulcatus ; CPUE ; Catch ; Shrimp ; Biomass ; Fishery ; Species
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 56pp.
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  • 42
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    Investigation on production frozen minced fish meat and frozen fish ball from Abramis brama (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: To study the preparation of frozen minced meat from abramis brama orientalis commonly known as sim from Arras lake and from silver carp cultured in the Azerbaijan province, and later this minced meat converted to fish ball and fish burger, in this process microbial, chemical and organoleptic tests was done on samples for frozen minced meat on Sims fish the TVN was 10.1 in zero phase and 14.1 after three month and peroxide value from 0.9 t0 3.1 at the same duration. But he value was higher when converted to fish ball, TVN ranged from 14 to17.6 and per oxide from 1.1 to 4.7 after three month, the unit for TVN was mg/100gram and peroxide unit meq/1000 gram, the microbial tests was also resulted in an acceptable range, the meat of Sims fish had no significant difference with the silver carp comparatively only the color and the smell of the fish was little different, so we could conclude that we can use Sim fish meat for frozen minced meat product and this can increase the consumption of fish in the region in the future.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Microbial ; Chemical ; Minced fish meat ; Frozen fish ball ; Abramis brama ; Silver Carp ; Culture ; Oreganoleptic test ; Samples
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 39pp.
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  • 43
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    Bacteria effects (probiotics) on growth and survival rate and resistance of shrimp during propagation stages (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This project investigates bacteria effects as probiotics on growth and survival rate and resistance of shrimp during propagation stages. Bacterial flora were extracted from seawater , water of cultured farms , shrimp cultured and sludge from private propagation farms different bacteria such as Vibrio alginolyticus(serotype1-4) Vibrio splendidus I , Vibrio fluvialis ,Vibrio anguillarum ,Vibrio costicul, Vibrio vulnificus,Vibrio nereis,Vibrio camplbelli,Vibrio natriegens,Vibrio proteolyticus, Vibrio plegius ,Vibrio fischeri,Aeromonas hydrophila, Aeromonas schuberti,Aeromonas salmonisida, Aeromonas veronii , Pseudomonas pseudoalcaligenes, Pseudomonas chloraphis, Alcaligenes faecalis, Bacillus megaterium, Bacillus pumilus, Bacillus circulans, Bacillus cereus, Bacillus subtilis, Bacillus licheniformis, Lactobacillus case, Lactobacillus bulgaricus, Lactobacillus delbrukii, Lactobacillus fermentum, Lactobacillus leichmanii, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus lactis, Pediococcus acidilactici were isolated , identified and lyophilized.These bacteria in different experiments were used on shrimp life cycle stages and live food such as Chaetoceros , Skeletonema and Tetraselmis algae. Some serotypes of one bacterial species showed various reactions in media and experiment probiotics. Vibrio alginolyticus(serotype1) with 107 cells/ml can increase Tetraselmis sp production during 6 days to 71% compared to the control experiment without bacteria. Also Vibrio alginolyticus(serotype4) with 105 cells/ml can increase Tetraselmis spp production during 6 days to 389% more than control. Vibrio splendidus could increase 23% shrimp fork length in postlarvae 3 compared control. Vibrio alginolyticus+ Vibrio fischeri can increase survival , fork lenght and body weight of green tiger shrimp postlarvae significantly(P〈.05).
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Bacteria ; Shrimp ; Algae ; Probiotic ; Vibrio alginolyticus ; Vibrio splendidus ; Vibrio fluvialis ; Vibrio anguillarum ; Vibrio costicul ; Growth ; Survival rate ; Resistance
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 85pp.
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  • 44
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    Broodstock from cultured Penaeus indicus for mass production of larvae (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: In order to produce broodstock from cultued shrimps , two groups of 1247 and 3781 ....
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Shrimp ; Production ; Larvae ; Broodstock
    Repository Name: AquaDocs
    Type: Report , Not Known
    Format: 97pp.
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  • 45
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    A survey on some risk factors and evaluation of their impacts on streptococcosis in rainbow trout farms in east of Mazandaran province (Haraz River) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: One of the most important bacterial fish diseases which has caused some outbreaks in rainbow trout farms in Iran is streptococcusis .The farmers were suffering from huge economic losses due to the disease outbreaks in different rainbow trout farms in Iran. The aim of our study was to determine rate of streptococcusis incidence in different stage of growth in farmed rainbow trout in Haraze River, Mazandaran province. Fish and water samples were randomly collected and measured of randomly in10 selected farms, monthly throughout a year. After clinical observations, Isolation and recognition of strep strains were made using biochemical tests. Some Environmental factors include Nitrate, Nitrite, Temperature, pH, Ammonia and DO measured during sampling periods. According to our results 4.6% of juvenile samples showed clinical singe of streptococcusis while only0.7% of them had strep. Contamination .These rates in adult samples were 8.9 and 1 percent respectively. Major isolated bacterial strain was Streptococcus uberis . Incidence of streptococcusis in rainbow trout 20% affected by fluctuation of Nitrite, temperature and DO. Management of these factors can decrease rate of disease outbreaks.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Clinical ; Environmental ; Streptococcusis ; Incidence ; Rainbow trout ; Environmental factors ; Survey ; Bacterial ; Diseases ; Samples ; Temperature ; pH ; Ammonia ; Juvenile
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 64pp.
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  • 46
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    The study of diversity, distribution and abundance of Zooplankton in the southern of Caspian Sea (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This survey was carried out by R/V Gilan with a conical plankton net of 100 micron mesh by vertical hauls at 8 transect in the southern Caspian sea. Transects were located at Astara, Anzali, Sefid roud, Tonekabon, Noshahr, Babolsar, Amirabad and Torkman in depths of 5, 10,20,50and 100m. Samples were taken in 4 seasons ( in each season 64 samples) in 2009. In this study 23 species formed the zooplankton community including 4 species of Copepoda, 8 species of Rotatoria, 2 species of Protozoa and 8 species of Cladocera. The meroplankton blonged to 2 species of Balanus sp and Lamellibranchiata larvae. The annual results revealed that the maximum abundance of Copepoda were 3612±4839 ind/m3 and 35/75 ±42/81 mg/ m3 in summer and decreased gradually since autumn and reached to minimum value in winter. The maximum abundance of Cladocera was 300± 383 ind/m3 in spring and reached to less than 4 ind/m3 in summer and autumn. The Rotatoria had high population (3775± 8632 ind/m3) in winter which consisted the zooplankton population with Copepoda. The Protozoa had no efficient role in zooplankton population of Caspian sea during the year. The zooplankton population was affected by Meroplankton and Lamellibranchiata larvae in spring and winter. The Copepoda contributed in zooplankton population only in summer and autumn. The maximum zooplankton abundance and biomass were in 5m depth which included 10209±8352 ind/m3 and 43/56±26/08mg/ m3 in spring, 8545±7127 ind/m3 and 73/59±56/65mg/ m3 in summer(10 m depth), 8427±6711 ind/m3 and 43/15± 29/59 mg/ m3 in autumn and 24426 ±26975 ind/m3 and 334/25±392/21 mg/ m3 in winter which decresed from surface to depth. The maximum abundance and biomass of zooplankton were 5119±6018 ind/m3 in east and 28/59±24/79 mg/ m3 in central area in spring, 6628±7117 ind/m3 in west area and 50/73±59/52 mg/ m3 in central area in summer, 5248 ±5489 ind/m3 and 31/16 ±31/22 mg/ m3 in west area in autumn and 11588±16191 ind/m3 and 131/02±193/61 mg/ m3 in west area in winter. The annual statistical analysis reveals that there is significant difference between Copepoda and Cirripedia population among seasons, sampling stations, depth and sampling layer (Kruskalwallis test, p〈0.05) and the total zooplankton populatin had no significant difference only between seasons (Kruskal-wallis test, p〉0.05). The Cladocera, Lamellibranchiata larvae and the Rotatoria had significant difference between seasons only (Kruskal-wallis test, p〈0.01).
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Zooplankton ; Copepoda ; Meroplankton ; Survey ; Plankton ; Samples ; Species ; Cladocera ; Meroplankton ; Balanus sp. ; Lamellibranchiata ; Larvae
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 123pp.
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  • 47
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    The comparative of histopathological study of different organs in vaccination, nonvaccination and exposed to white spot disease in shrimp Litopenaeus vannamei during the study (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: White spot syndrome virus (WSSV) is the causative agent responsible for huge-shrimp viral epidemics in shrimp farms throughout the world.Our study was aimed to determine the effect of WSSV Gamma-vaccinated Litopenaeus vannamei in occurrence of apoptosis. One thousand and twenty PL15 were randomly distributed among 2 treatments and two control groups. Gama-Vaccinated shrimp and none-Gamma-vaccinated ones were our treated and untreated groups. Our result showed there is a significant difference (p〈.05) between vaccinatedexposed group (82.33±2.51) and none-vaccinated exposed group (26.00±10.00) was about 56%. It is concluded that apoptosis can be a helpful process as immune function in shrimp especially against WSSV.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Histopathological ; Vaccination ; White Spot Disease ; Shrimp ; Litopenaeus vannamei ; WSSV ; Gamma ; Immune
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 30pp.
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  • 48
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    Effects of Unsaturated Fatty Acids on the Reproductive Performances of White Indian Prawn ( Rindicus ) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The shrimp farming success in the future will greatly denpend upon increasing supplies of healthy and high quality seed for stocking ponds. The limited and inconsistent availability of wild Shrimp postlarvae together with an urgent need of establishing selective breeding programs, increased the interest in captive broods of penaeid. Many investigations have been carried -out A on several shrimp species world-wide. This study have been conducted on 2 shrimp groups since September (Mehr) to July (Tir) 1999 in Bandar-e-lmam southwest Iran. The wild group (SPB) prepared from Jask area and the pond group (POB) prepared from artificial ponds upon harvesting them as marketable. size have been investigated. 2 groups were exposed to natural diets consisting of squid and artemia biomass in same conditions using 12 m concrete tanks. Stocking density was 2 piece/m2 (IF: IM). Half part of the pond bottom covered by fine sand to reduce the stress where the other part has been used for feeding. Daily ration given in 3 times by satiation method. Also reproductive performance of wild gravid brooders (WLB) were recorded as a control group 2 examined groups well grown and around 50% of the SPM and around 30% of POB females successfully ripened an produced eggs an nauplii where almost all males of both groups were matured. Data showed fecundity is highly correlated to the TL and W of the brooders by positive trend. Considering the T WI Fecundity and nauplii production the WLB brooders are significantly. (P=0.05) higher than 2 other groups, while the SPM broods are significantly (P=0.05) higher in hatchability. Meanwhile the survival rate from nauplii to postlarvae stage was around 30% for SNIP brooders. On the other hand the growth parameters (TL W) of the marketable size of the shrimps produced for SMP brooders were significantly (P=0.05) higher than those produced from WLB group. Since the fecundity is improvable by increasing the brood size thus the data suggest the superiority of the SPM brooders.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Shrimp ; Postlarvae ; Females ; Males ; Growth parameters ; Fecundity
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 51pp.
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  • 49
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    Efficiency of shrimp trawl nets polyamide and polyethylene with standard mesh in the coastal waters of Bushehr Province (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This study was carried out in August – september 2009 in Bushehr province waters. Two different shrimp bottom trawls were used in which were woven by Poly Amid and Poly Ethylene with mesh sizes( stretched ) of 50 mm and sack with 35 mm. Also 2 different vessels of two dhows and one steel ship each with 26 and 30 hauls were used and taken in to comparison from point of obtained results. Total catch of PA and PE trawels were 4592 and 5535 kg of which the ratio of shrimp catch and bycatch for PA trawl was 32.4 and 67.6 % respectively; and for PE trawl was 34.2 and 65.8%. Total catch of PA and PE trawls of steel trawler ( ship ) were estimated 3158.0 and 4784.7 kg, respectively, of which the shrimp/bycatch ratio was 6.6 : 93.4%. There was a higher amount of bycatch for PE comparing to PA trawl (p〈 0.05). on the other hand the decrease of shrimp and bycatch for PA comparing to PE dhow trawlers were 21.3 and 15.0% and for ship were 34.6 and 34.0% respectively. The weight ratio is 1:15 i.e . in PE ship trawl , in proportion to on kg shrimp, 15 kg bycatch was harvested. it is proposed to standardize the mesh size and also to do obligtory the instal of squared mesh size panel .
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Shrimp ; Poly Amid ; Poly Ethylene ; Weight ratio ; Mesh size ; Obligtory ; Trawling
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 74pp.
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  • 50
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    Determining the best method for fishing Portunus Pelagicus in Chabahar waters (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The blue swimming crab Portunus pekagicus linneus, 1758, is Known as a by catch of trawlers, Gillnetting,... in the coastal waters of the Persian Gulf and Oman Sea, Blue crabs are not considered as target catch in the Iranian fisheries. The best method for catching these crabs is in this survey. Two kind of posts including Japanese shrimp pots and collapsible pots were tried. Also gill net was used. Result showed that Japanese shrimp pot and collapsible pots were determined as the best gear, Samples catched by these pots have larger mean lengths and weight, they are mainly undamaged.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Fishing ; Portunus pelagicus ; Shrimp ; Crabs
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 64pp.
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  • 51
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    Monitoring of Environmental Impacts of Shrimp Pond Development Tiab district (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The present survey was conducted for evaluation of environmental impacts of shrimp farm effluents in Tiab district of Hormozgan province. During the growing season of 2003(Jul-Dec) water and sediment samples have taken with monthly intervals from inflow and outflow of the farms and related coastal waters adjacent to Tiab estuary. The samples were carried to the laboratory for physical, chemical and biological analyses. The results showed that the farms exert significant influence on humidity of surrounding air. Furthermore most of the measured parameters such as pH, salinity, BOD5, COD, ammonia, nitrite, nitrate, phosphate, TP, total phytoplankton and zooplankton numbers in water column and the percentages of the TN of sediments of the outflow waters were adequately higher than inflow water, but reverse results were measured for oxygen content of the water and total macrofauna numbers. There were no any significant differences between inflow and outflow channels in the case of temperature and TN of the water. In the statistical analyses, station effect only showed significant influences on variation of zooplankton numbers, TN, COD and salinity of the water and the percentage of sediment total organic compounds. The results also showed that during the growing season in the surveyed channels the most values of water and air temperature, air moisture, salinity and COD decreased, but in contrast the magnitude values of pH, BOD5, nitrite, nitrate, phosphate, TP and total phytoplankton and zooplankton numbers increased during the same time. The month effect significantly influenced the preceded noticed parameters, but had no significant effect on variation of other parameters such as: TN and total macrofauna of the water column and percentages of total organic compounds and nitrogen of the sediment. The results showed that there were no significant differences between the surveyed channels and coastal waters in the case of measured values of water and air temperature, pH and oxygen content. Higher total phytoplankton numbers were measured in the coastal waters, but most of other surveyed parameters were high in the channels. In the coastal waters the variations of most measured parameters were managed by winds and the related waves and turbulences, so water exchange with Tiab estuary had no significant effect in this respect.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Monitoring ; Environmental Impacts ; Shrimp ; Pond ; Evaluation ; Phytoplankton ; Zooplankton ; Coastal waters
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 65pp.
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  • 52
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    Effect of different heating treatments and kind of filling media on the amounts of some mineral elements (iron, zinc, copper, calcium and sodium) of silver carp (Hypopthalmichthys molitrix) during canning (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: In this study, first, the influence of three precooking methods (steaming, oven-baking and microwave-cooking) on the contents of mineral elements-copper, zinc, iron, calcium and sodium-of silver carp (Hypophthalmichthys molitrix) was evaluated. Determination results of evaluated elements in raw fish and cooked samples were in range 3.05 to 4.19 for copper, 71.45 to 82.85 for zinc, 32.18 to 40.70 for iron, 425.6 to 529.46 and 315.5 to 534.76 for calcium. Results showed different precooking treatments had no significant effects on the amounts of mineral elements such as copper, zinc, calcium and sodium. The Iron content in the samples subjected to microwave cooking increased. With doing multivariate analysis, on comparing the raw and precooked fillets, steam cooking found to be the best precooking method on retain mineral elements. After choosing the best precooking method, for evaluating the influence of different filling media including sunflower oil, soybean oil, olive oil and brine, on the quality of canned silver carp, amounts of mineral elements(copper, zinc, iron, calcium and sodium) and microbial indices(total counts, thermopile count and clostridium) and sensory indices (color, smell, taste and texture) was surveyed. Assessments of microbial indices showed no microbial activity in canned products. After canning amounts of sodium increased in all the treatments. Except for iron, using soybean oil had no significant effect on the contents of other elements. The highest levels of iron and copper were observed in olive oil canned samples sterilized at 130°C. Sensory evaluating showed, kind of filling media had no significant effects on sensory indices such as taste, smell and color of canned samples. The texture of soybean oil canned samples and the quality defects of olive oil and brine canned samples had better condition than other treatments. In the last step, canned silver carp were proceed under three different temperatures (115°C, 120°C and 130°C) with equal lethality value (Fo=7min), then sensory indices and amount of mineral elements were compared. Results showed, the contents of iron, copper, sodium and calcium were changed in soybean oil canned sample. The amounts of copper and sodium in sunflower oil canned sample subjected to different heating regimes showed significant variation. The highest amount of copper was observed in the canned samples subjected to 130°C heating regime. Results showed contents of iron and copper of olive oil canned sample subjected to 120°C and 130°C heating regimes were higher than sample subjected to 115°C heating regime, while the zinc and calcium contents had no variation. In brine canned samples the highest amounts of copper and iron was obtained after sterilization in 120°C. Results of sensory evaluation showed different heating regimes had no significant effects on the indices of taste, smell, and color of products. Doing 130°C heating regime in brine canned samples led to obtain the better tenacity of texture. While this heating regime caused to increase the quality defects of soybean oil canned samples as a result of existence of hard parts of bone.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Precooking ; Filling media ; Heating regime ; Sterilization ; Silver carp ; Mineral elements ; Hypopthalmichthys molitrix ; Samples ; Sunflower oil ; Soybean oil ; Silver Carp
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 109pp.
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  • 53
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    Global population structure of the great sturgeon (Huso huso) in the southern part of the Caspian sea with emphasis on the Golestan Province (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Report of the project entitled "determination of the global population structure of the great sturgeon (Huso huso) with emphasis on the Golestan Province" is in front of your attention. Outcome is the results of study on the samples collected from the total catch in the landing sites namely Bandar-e-Anzali, Babolsar, and Ashooradeh, in the three Northern provinces. 224 specimens were collected according to the sampling manual during the fishing seasons in 2005 and 2006. Three methods were applied in this study to find out the population structure of the great sturgeon in the southern Caspian Sea. These were as follows: Morpho-metric meristic Population dynamics Population genetics (microsatellite loci) Results indicate that the range of different age groups of the great sturgeon in the Golestan Province is larger than the other two provinces. Results show that the older age groups are found in the samples from the Golestan Province rather than the other two provinces. Results demonstrate that the sample size for the two methods (morpho-metric meristic and population dynamics) is not enough. In order to obtain precise results with less uncertainty one needs to collect more specimens. Therefore, according to the few number of the great sturgeon in the total sturgeon landing, it is recommended that researchers apply methods other than the two methods which are mentioned above in order to make sure that the results are most reliable. Population genetics methods including microsatellite loci are among the methods which could produce good results with minimum bias in this regard. Results indicate that there are two distinguishable populations of the great sturgeon (Huso huso) in the southern part of the Caspian Sea off the shores of the three Northern provinces. Results also show that these two populations are scattered in the two east end (Golestan Province) and west end (Guilan Province) of the southern Caspian Sea. The two populations have overlapped each other in the Mazandaran Province.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Population ; Sturgeon ; Huso huso ; Sampling ; Morpho-metric ; Samples ; Meristic ; Genetic ; Microsatellite ; Specimens
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 79pp.
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  • 54
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    Study on breeding of imported brood stocks Litopenaeus vannamei in Iran environment (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Regarding to breeding the Litopenaeus vannamei, a total of 126 pairs of broodstocks were imported from Hawaii to Iran in 2004 and 2005 , and then transferred to the Bandargah Research Station in Bushehr. The female broodstocks were ablated, and were feeded 3 times per day with cuttlefish ,small size shrimp and Nereis worm with a ratio of 30% body weight .The water exchange were done 3 times per day. During the years 2004 and 2005 a total 1700000 naupli were produced of which 772000 specimens of pl13 and pl7 were harvested and then transferred to Helleh Site for carrying out the next culture project. The average naupli and postlarvae were 170000 and 92000 in proportion to each broodstock. Also the mean survival rate was estimated 54%.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Litopenaeus vannamei ; Broodstock ; Breeding ; Female ; Cuttlefish ; Shrimp ; Nereis worm ; Body weight ; Nauplii ; Specimens ; Survival rate ; Breeding
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 36pp.
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  • 55
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    The study of propagation and culture of marine worm (Perinereis nuntia) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Marine polychaete worms living in different beds from coastal areas, so different cultures can be different effects on their development. The evaluation of this research initially, marine polychaete worms were collected in low tidal sea areas from Gulf Persian coast in Bushehr. During several stages of sampling do more than 2000 pieces of worm were taken and then transferred to Bandarga Shrimp Research Station (Iran Shrimp Research Center). So based on a observation the desired species identification (Perinereis nuntia). Then 400 pieces worm selected and transferred to tanks maintain immature worm (fiberglass tanks with dimensions of 2 × 2 × 0.5 m with 15 cm sand on floor). The results showed that even the worms (P. nuntia) in vitro reached sexual maturity able to spawn in captivity conditions and were absolute fecundity of 16010 ± 17630 eggs. After incubation eggs, larval culture into the plastic tank with 70 cm diameter, larval (1.5 to 2 mm) were introduced with density 2000 (pieces/m2). In this research used to three different beds ( tidal , filled with water all of time and wet bed ) with three different food types (food shrimp, seaweed and vegetable waste), so there were 9 experimental treatments. All experimental treatments harvested and estimate production in each of them after 270 days. The seaweed tidal treatment with amount of 21.17 g/m2 had best results and the all of wet treatments with 0 g/m2 was the lowest production. Compare of beds showed that the tidal treatments have significant difference with other beds in growth factors (e.g. mean weight, survival percentage and total production) (P〈0.05). And for effect of food kind on growth factors, the result of difference foods in tidal treatment were analyzed. According this mater any treatments have no significant difference together in growth factors (e.g. mean weight, survival percentage and total production) (P〉0.05).
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Experimental ; Propagation ; Culture ; Marine ; Worm ; Perinereis nuntia ; Polychaete ; Sampling ; Shrimp ; Perinereis nuntia ; Immature ; P. nuntia ; Sexual maturity ; Survival percentage
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 76pp.
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  • 56
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    Designing and establishment of ISO/IEC17025 in the research centers affiliated to IFRO The first phase: The Persian Gulf Ecological Research Center, Caspian Sea Ecology Research Center and Iran Shrimp Research Center (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The plan entitled "designing and establishment of ISO/IEC17025 in laboratories of three research centers affiliated to Iran Fisheries Research Organization" was carried out in the frame of three projects from March 2008 for about three years. Among the laboratories of The Persian Gulf ecological research center, the Caspian Sea ecological research center and the national shrimp research center 7, 5 and 3 laboratories were selected for accreditation, respectively. The quality manuals of the test laboratories were written following the requirements of ISO/IEC 17025. During the establishment of the system standardization of the test methods, calibration of the equipment, test methods validation and update and improvement of physical conditions of the laboratories were performed. The technical and quality management competence of the laboratories affiliated to the Persian Gulf ecological research center were evaluated by the auditors from DGA (the German Accreditation System for Testing Laboratories), which is signatory of ILAC (International Laboratory Accreditation Co-operation). But in the case of the two other centers, the laboratories were accredited by Iran Accreditation System (IAS). The laboratories officially accredited, after the successful completion of the accreditation process (including implementation of corrective actions for the detected non-conformities). Production of reliable results of the test methods from national and international scientific organizations' point of view as well as facilitation in development of bilateral and multilateral relations with them can be considered as the advantages of the system establishment. Moreover, exchange of the relevant scientific information and experiences among the researchers will be facilitated
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Ecological ; Physical ; ISO/IEC17025 ; Shrimp ; Standardization ; Laboratories ; Fisheries ; Iran Shrimp Research Center ; Caspian Sea Ecology Research Center ; Persian Gulf Ecological Research Center
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 30pp.
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  • 57
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    Determination of Oil pollutant (water, sediment and fish) in the southern part of Caspian Sea (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This study was conducted to determine 16 Polyaromatic Hydrocarbons (16PAHs) concentrations in water (during four seasons) and surface sediments (during summer and winter) at eight transects (Astara, Anzali, Sefidroud, Tonekabon, Nowshahr, Babolsar, Amirabad and Turkman) in the southern of Caspian Sea in 20102011. 94 samples of water and 45 samples of surface sediments were collected at 10, 50 and 100 meters depths. In addition, 28 samples of fish (Cyprinus Carpio) were collected during winter and spring. All samples were prepared by Soxhlet and extracting processes and then determined using High Performance Liquid Chromatography (HPLC) instrument. Results of current study showed that mean concentration and standard error (±SE) of 16PAHs water were observed 232 (±77), 1268 (±808), 538 (±190) and 151 (±53) µg/l in spring, summer, fall and winter, respectively. In addition, annual mean contents and standard error (±SE) of 16PAHs water were registered 24.10 (±8.12) µg/l. The Hazard Quotation (HQs) were calculated more than unit and belong to Benzo(a)pyrene and Dibenzo(a,h)anthracene compounds. Mean concentration and standard error (±SE) of 16PAHs sediment were observed 0.77 (±0.23) and 1.21 (±0.64) µg/g.dw in summer and winter, respectively. Also, the annual mean contents and standard error (±SE) of surface sediments were observed 0.93 (±0.33) µg/g.dw. The Hazard Quotation (HQs) were calculated more than unit and belong to Fluoranthene, Benzo(a)anthracene, Chryseneand Benzo(a)pyrene. The annual mean contents and standard error (±SE) of edible tissue of Cyprinus Carpio mussels were observed 2.21 (±0.42) µg/g.dw. Annually, pattern of 16PAH compounds were obtained 10, 70, 12 and 8% for 3,4,5, and 6 rings, respectively. Four ring compounds had high content and percentage and 2 ring was not observed in all samples of water. Also, pattern of 16PAH in surface sediments were obtained 31, 56 and 14% for 3, 4, and 5 rings, respectively. Four ring compounds had high content and percentage and 2 and 6 rings were not observed in all samples of surface sediments during two seasons. Result of Diagnostic Ratios analysis (DRs) of sediments showed that the source of oil compounds were petrogenic and pyrogenic in summer and winter, respectively. Based on DRs in water and sediments were observed oil compounds were either petrogenic or pyrogenic with different percentage in the southern Caspian Sea. In conclusion, the results revealed that in the some transects the 16PAHs concentrations of water were above the threshold levels and more petrogenic (85%) sources which represented polluted condition in this area. 16PAHs concentrations of sediments were below the threshold levels and conditions for this region were showed unpolluted. Also, the Caspian Sea sediments were classified in Class 2 (Fair). According to results of the evaluation’s risks to human health associated with consumption of the mussels containing 16PAHs suggest that there is risk for humans.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Oil pollutions ; Water ; Sediments ; Fish ; Cyprinus carpio ; Samples ; Polyaromatic ; Hydrocarbons
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 78pp.
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  • 58
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    Possibility of trout farms efluent return to groundwater In non-agricultural seasons (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This project is trying to investigate water contamination after use in ponds. For this aquaculture status, physical and chemical water properties, microbial pollutants and heavy metals levels in the some of trout ponds effluent in East Azerbaijan province were evaluated in 2007 to 2009. The information of groundwater and climate conditions in ten years, Drilling and wells piezometric Information, in local wells are reviewed.According to data obtained from analysis of water samples in the output pools range studied concentrations(term mg/l) some parameters are as follows:(BOD: 1.9-3.2),(COD: 3.4-34.1),(TSS: 26.6-42.7),(N-NO2:0.003-.136),(P-Po4:0.0170.067),(pH:7.75-8.28).Electrical conductivity(500 ±2 to 1129±144 term µm/cm) in the effluent ponds . Based on the results of we can say that between input and effluent ponds in the review of all factors as chlorine, sulfate, sodium, potassium, total nitrogen, soluble phosphorus, total phosphorus, calcium, total hardness, magnesium, alkalinity and silica significant changes not be observed. Review of water analysis data to some factors(such as:water temperature,EC,pH,NO2,CO2,HCO3,CO3,DO,COD,BOD) And compare this data with the authorities standard confirmed that the All factors mentioned in effluent is allowed now. In terms of microbial contamination and heavy metals ( Zn,Cu,Ca,Pb,Fe,Cr ) is no pollution. Appropriate strategies for removing suspended materials must to use.We can use Watershed Management solutions to inject output water to the underground table. Should be noted status change (eg change in flow rate, an unusual increase in temperature, fish density increasing, reducing food quality, lack of appropriate management actions and ...) may reduce the effluent quality and the ability of injection water underground to lose . In this case, measures must be for wastewater treatment and improve the quality ponder.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Physical ; Chemical ; Microbial ; Effluent Trout ; Return ; Ground Water ; Non-agricultural ; Aquaculture ; Pollutants ; Heavy metals ; Samples ; Chlorine ; Sulfate ; Sodium ; Potassium
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 88pp.
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  • 59
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    Sperm bank creation of Caspian Sea trout (Salmo trutta caspius) breeders (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The Caspian Sea trout (Salmo trutta caspius) is distributed in southern basin of the sea and the natural stocks of this fish is critically endangered. The present study was conducted through the breeding seasons of Caspian Sea trout in January 2012. Estimation of sperm male potential was carried on samples collected from a total of 12 male spawners which included 8 samples (caught 3 years ago) and 4 samples (freshly caught). Semen samples were collected after anesthetized with clove powder for 10 minutes at water temperature of 9-9.4 °C. The differences of mean semen volumes, sperm motility time, sperm density and the percentage of sperm motility from freshly caught spawners ( 5.5 ± 3.4 ml , 35.5 ± 8.7 sec , 4.3 ± 0.7 ×109 ml-1 , 32.5 ± 8.5% respectively) were significantly higher than 3 years caught spawners (1.5 ± 0.93 ml, 26.5 ± 12.8 sec , 1.98 ± 1.6 ×109 ml-1 , 25.7 ± 9.7 % respectively) (P 〈 0.05). Also the results showed that there are not significant differences in mean percentage of motility and motility duration between freshly caught sperm from fresh broods and that’s from previously caught (among fresh sperm samples, one, three and eight months of cryopreservation) (P 〉 0.05).
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Sperm cryopreservation ; Percentage of motility ; Sperm bank ; Salmo trutta caspius ; Breeding ; Samples ; Spawners ; Temperature
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 31pp.
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  • 60
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    Identification and distribution of mullets in the coastal water (intertidal zone) of Bushehr province northern Persian Gulf (2008-2009) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The distribution and stock assessment of edible bivalves were carried out in Bushehr shoreline areas (from 50֯ 38 - 29֯ 27 to 52֯ 41 - 27֯ 17 ) throughout the period of September 2008 - 2009. The objectives of this study were identifying the location of edible bivalves, biomass estimation, to estimate the growth parameters and natural mortality. Samples were obtained at roughly monthly or bimonthly from 7 areas and some of the at low tide during the year from 4 areas. Transects were selected at random direction and at the zigzag pattern in every area, so that is cover all of the shell beds. In each transects one or two sampling quadrat (0.25 m2) were placed randomly. Depending on the area size the number of the quadrat were different. After the removal of the stones and debries, the edible bivalves (empty and live bivalves) and some of the decoration shells were identified and numbered. Some of the samples were transferred to a bag and taken back to the lab for further identification and further analysis. In this study, the percentage of samples was used to show the distribution of the species in different areas. Faunal affinity between stations was calculated by Sorensen formula. For the purpose of the stock abundance, the mean number of the shells in each quadrat was regarded as an estimate of stock mean. The total stock in the investigated areas was obtained by multiplying the mean by the ratio of the stock area to the quadrat area (0.25 m2). Confident limits 0.95 % or 95 probility was given for precise the estimates. The length of some of the bivalves was measured from dextral to sinistral with vernier calipers to the nearest 0.1 millimeter. In some of the species the length frequency was used to estimate growth and natural mortality parameters. A subset of samples of Solen brevis was taken to the laboratory for the precise measurements, and relationship between body weights and total length. Surface sediment samples (5-20 cm) inhabited by some clam species were sampled in some areas. Water temperature and salinity were recorded during the survey in the sampled areas. A total of 45 species or species group was identified in the study area. The main area distributions for live edible bivalve, Solen brevis were located at Bupatil, Kaloo, Piazi and Bordekhoon. Other bivalve, Paphia cor was distributed in the Gassir, but empty bivalve shell was seen in the Bordekhoon shoreline. A limited tidal coastal area of Ganaveh has been covered by a pearl oyster species, Pinctada radiate that attached in the rocky beds. The empty shells of this species and other genus of the family were distributed in the other areas such as Golestan and Nayband. The most important areas for the decoration shells were located in the Golestan and Nayband. The empty bivalve shells, Trachycardium lacunosum were distributed in Lavar shoreline. The other species or species group were distributed in the shoreline of the study areas in different months with the low abundance. The growth parameters of two alive species, Paphia cor and Solen brevis were estimated. The Von Bertalanffy growth parameters for Paphia cor were estimated as K = 0.8 Year-1, L∞= 55 mm and t0 = -0.45 and for Solen brevis K = 0.7 Year-1, L = 120 mm and t0 = -0.35. . Estimated natural mortality rate for Paphia cor was M = 0.57 and for Solen brevis M = 0.26. The maximum ages (Tmax) calculated were 50 months for Paphia cor and 54 months for Solen brevis. The mean biomass of Solen brevis in Bupatil was 3.25 1.1 shells per quadrat with a maximum peak 4.6 shells per quadrat in October (Mehr) and minimum 1.5 shells per quadrat in Augest (Mordad). For the pearl oyster, Pinctada radiata in the Ganaveh this estimation was obtained in maximum peak in July (Tir) 60.5 shells per 100 m2 and minimum in March (Esfand) 3 per 100 m2. Mean biomass for this species was estimated 27.724.2 shells per 100 m2. The mean biomass of P. cor was 8.8 2.2 shells per quadrat and with a maximum in February (Bahman) and minimum in November (Abban).
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Identification ; Distribution ; Mullets ; Stock assessment ; Biomass ; Growth ; Mortality ; Samples ; Species
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 80pp.
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  • 61
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    Survey of petroleum pollutants in shrimp habits of Hormazgan Province (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The purpose of this study was to evaluate Total petroleum hydrocarbons (TPHs) in surface sediments of Khamir, Laft, and Yekshabeh and Tiab esturies. For this purpose, sampling of surface sediments was carried out at 16 stations (four stations at each estury.In each sample, four points were taken from the beginning, the middle, the end and the junction point to the sea.The physicochemical properties of sediment such as grain size and total organic matter (TOM) were measured using standard methods. The concentration of total petroleum hydrocarbons was determined using Ultraviolet Fluorescence (UVF). Petroleum hydrocarbons showed limited variations from 53.11 to 2052.5 ng/g dry weight in the summer season and from 128.5 to.4392.5 ng/g in winter. The average detected concentrations for total hydrocarbons were lower than the environmental hazard (4000 ng/g). Most of their concentrations were observed at Yekshabeh estury in winter, possibly due to the pollution caused by the activity of ports and fishing vessels. Also, the assessment of environmental hazard caused by pollution of hydrocarbons for aquatic organisms by using SQSs and contamination coefficient (Cf) showed that the studied sediments except station 1 at Yekhshabeh estury in winter season (Cf = 0.91) has no adverse biological effects on aquatic organisms. Finally, the results of statistical analysis of the data showed that the physicochemical parameters of sediments (grain size and total organic matter (TOM)) and the total petroleum hydrocarbons were not correlated, which could indicate the existence of different sources for each studied parameters.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Survey ; Petroleum pollutants ; Shrimp
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 58pp.
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    Recognation of fishes in rivers and basin of Qom (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This study was carried out on rivers, basins & qanat of Qom region during the 1376-1378and Fish samples were taken from 2 permanent rivers (Qomrood ,Ghara_chag)& from some seasonal ones (Tagharood, Zavarian, Vesva, Biraghan). Also local fishes was followed in 100 qanats in the region and Random fish sampling was done in permanent river extension out of province. This study aimed to recognize different fish species in the province water reservoir and estimating the fisheries potential in the province. For fish sampling nets such as mashk, salik, hook and hand tailored were used. Electroshoker was not used at all. Results of fish recognition showed that fish samples belong to 12 species & 4 family of "cyprinidae", "Balitoridae", "cyprinodontidae" and "poecilidae".The qanat fishes of the montain (kahak, Khagestan) and pastora area (Ghanavat,jafarabad) included by two families of "cyprinid", "Balitoridea".In some qanat which were connected to rivers more species of fish and anotherAquatic animal were observed & sampled.At the and map of geographical distribution of local fishes was planned.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Geographical ; Fish ; Samples ; Species ; Fisheries ; Electroshoker ; Cyprinidae ; Balitoridae ; Cyprinodontidae ; Poecilidae ; Aquatic ; Recognation
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 67pp.
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    Using Bio-Chemistry Genetics Markers to Distinguish Barbus in Khozestan (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: 0Genetic polymorphism by means of biochemical genetic markers using polyacrylamide gel electrophoresis system in four barbus fish species has been investigated. Species scientific name were Barbus sharpeyi ; Gunther, 1847 , Barbus grypus ; Heckel, 1843, Barbus xanthopterus ; Heckel, 1843 and Barbus esocinus ; Heckel, 1843.Sampling site and location were mainly in the rivers located in Khoozestan province such as Karoon and Karkheh rivers and the Dez dam. Different organs such as blood, muscle, kidney, eye and heart were sampled and analysed for the presence of tf, es, sod and pgm alleles. High polymorphism and presence of different alleles scored, but populations were not in the H-W equilibrium. Considring results, using current and avaiable genetic markers such as microsattelits is recommended for future works.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Chemistry ; Genetics ; Genetics Markers ; Distinguish ; Barbus ; Species ; Barbus sharpeyi ; Barbus xanthopterus ; Barbus esocinus
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 62pp.
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    Biological and Reproduction behaviour of Eleuthronema tetradactylum (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: In this study spawning season, brood stocks live place and biological behaviour of Eleutheronema tetradactylum (rashgoo) was identified in the coastal waters of Bushehr province, Persian Gulf from July. 2005 to Sep. 2006. During this time fish samples were collected by the gillnet or set net. With the mesh size of 9 and 25 Cm. A total of 93 fishes were examined in the fishery Research Centre laboratory, Standard length, total weight, gonad and hepatic weight were measured and histological studies on gonads were conducted in this period. Gonadosomatic index was calculated in the different seasons we showed significant differences between research seasons (P〈0.05) the Maximum GSI was in winter and spring with (1-1/2 %) and minimum was in summer and autumn (/15-/22 %). In histological study of ovary had observed five stages in sections: IMMATURE, INITIAL MATURATION, ADVANCED MATURATION, MATURED and SPAWNED. And testicular tissues were showed three stages of the sperm development: Spermatogonia, Spermatocytes and Spermatides or sperm. This study had showed that Higher quantities of brood stock were caught in DAYER region of Bushehr province. The stomach contents observed that small fishes, shrimp and crab were feed by the caught fishes, it means that Eleutheronema tetradactylum (rashgoo).is a carnivorous fish. The results were obtained from this research showed that reproductive season in Four finger thread fine (Eleutheronema tetradactylum) (rashgoo) in the coastal waters of Bushehr was in the could seasons.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biological ; Histological ; Behaviour ; Eleutheronema tetradactylum ; Spawning ; Ovary histology ; Broodstocks ; Samples ; Gillnet ; Gonadosomatic Index ; Immature ; Seasonal spawning
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 51pp.
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    Stock assessment of Khouzestan fishes (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Catch/effort statistics of Khouzestan Province collected from Shilat Statistical System in 1379 have been analyzed. Trends in Khouzestan fisheries from 1372 until 1379 have been also investigated, Length frequency samples collected by catch observers in two main landing centers have also been analyzed. For this purpose, some of the most important commercial fish species were selected for length measurements, the highest no.. of fishing units were operating in Abadan area. Total no. of fishing uints in Khouzestan Province have been increased about 9.3% comparing to the previous year. Total NO. of fishing days were about 174486 days, from which 532% was excreted by speed boats and 46.8% by Lenj. Total landed catch was estimated to be about 24061895 kg which was 0.2% less than the previous year. The highest landed catches belonged to Mid, Otolithes ruber and Tenualosa ilisha respectively, estimated biologic parameters fir the selected species show that in most of the cases their exploitation levels are too high.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Stock assessment ; Fish ; Species ; Samples
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 75pp.
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    Shrimp culture, Litopenaeus vannamei in the closed recirculation system (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The project carried out at Shrimp Research station of Bandargah in 2011 to determine the production parameters such as; Average body weight, production, survival and feed conversion ratio in closed- recirculating aquaculture system for shrimp culture. This project was performed in 4 m3 fiberglass tanks. 3 tanks was chosen for the control and 3 were assigned to treatments. Considered Species in this treatment was white shrimp (Litopenaeus vannamei) that with a density of 60 pieces per square meter, and was reared for 90 days. culture in control as usual and change water regularly, but the treatments did not change water during the period of culture. Water in the treatment tanks was going to turn the filter system and waste was removed by mechanical and coal filter embedded in the route, and after filtering was transferred to the wastewater tank. Wastewater Was aerated in this tank and after 24 hours was pumped out and return to treatments tanks. In the final of project, mean weight, survival rate and production for control and treatment, was respectively, 18.27 ± 2.84 and 20.70 ± 1.47 g, 73.83 ± 4.14 and 77.17± 13.66 percent, and 0.81 ± 0.14 and 0.97 ± 0.23 kg/m2. Statistical analysis results show that there are no significant differences found between treatments and control (P〉 0.05), although in all the above criteria, the relative improvement was achieved to compared of control. But the results of feed conversion ratio was 1.27 ± 0.16 for treatments that are significantly different (P 〈0.05) with controls (1.56 ± 0.23). This result showed that efficiency of the food was better in closed- recirculating shrimp culture system.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Shrimp ; Culture ; Litopenaeus vannamei ; Weight ; Survival rate ; Species ; Feeding
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 83pp.
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    Investigating effect of different methods of eye ablation Penaeus semisulcatus for maturation (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: In order to study on this subject, we chosed four methods of eye ablation (pinching, coutery, cutting, ligation) and without eye ablation with 5 treatments and two repeat. We prepared 10 tank by normal method in broodstock. We stocked 15 shrimps in the ratio of 1:2(male to female) per tank. ln each tank all of condition such as salinity, temperature, pH, light, type and colour of and change of water was equal for all tanks. The only variable factor was methods of eye ablation. After six week - period results showed us that respectively (30,25,20,15 and 5%) of shrimps in treatments reached to stage 4 and spawned. lt shows that the best methods for eye ablation are pinching and cautery on Psemisulcatus.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Eye ablation ; Penaeus semisulcatus ; Maturation ; Shrimp ; Salinity ; Temperature
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 32pp.
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    Study of Parasites occurrence and intensity in fishes of Anzali Lagoon (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Tish survey prolonged from summer 2011 trough summer 2012. During this period 574 samples of different fish species were investigated for their parasites. Totally 30 species of parasites isolated of the fishes. Some of the isolated parasites are recorded for the first time in Iran. Rafidascaris acus Orientocreadium siluri, Silurotaenia siluri, Acanthocephalus lucii , Argulus foliaceus has recorded for the first time from European catfish, Silurus glanis, and Dactylogyrus inexpectatus has recorded for the first time from Gibel Carp, Carassius auratus gibelio, in Anzali wetland. Statistical comparison of parasites infections and intensity between the different area of Anzali wetland were done. Infection of Pike, Esox lucius, to Diplostomum spathaceum, Raphidascaris acus and Monogenean in western parts of wetland were significantly different from the Eastern and Central areas (p〈0.05). So based on the data have concluded the eastern and central regions of the wetland are more polluted than the western part. Comparing the results of the present study with before ones showed that the composition of parasite species has been changed over time, while the prevalence, intensity and abundance of parasites have been increased. It may be due to changing environmental conditions such as increasing discharge of effluent, eutrophication of the wetland. This results accents to necessity of reconstruction of Anzali wetland with preference of eastern and central regions.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Parasites ; Fish ; Infection ; Intensity ; Survey ; Samples ; Species ; Rafidascarisacus ; Orientocreadium siluri ; Silurotaenia siluri ; Acanthocephalus lucii ; Argulus foliaceus ; Catfish ; Gibel carp
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 42pp.
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    An ecological investigating on controling Mnemiopsis leidyi population activity 6: Molecular test in identification of Menmiopsis leidyi (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: In this study population genetic structure of Mnemiopsis leidyi were examined by 200 samples from the Caspian Sea. Samples were collected from Guilan province (Astara region), Mazandaran province (Amir Abad region), Golestan province (Gomishan region) and north part of the Caspian Sea. 50 samples were colected from each region. DNA was extracted by phenol - chlorophorm method and its concentration was 50 to 100 nanogram . PCR was performed using 18s rRNA gene. The PCR products of samples were digested by 12 restriction endonuclease enzymes. The digested products accompanied with standard marker (50 bp lader ). In order to measure fragment size samples were run on a 6% vertical polyacrylamide gel. The fragments were visualized by silver staining of the polyacrylamide gel. In this study, Dra I and Alu I enzymes showed different digestion pattern, as each of these enzymes had two genotypes. Other enzymes had similar digestion pattern. RAPD method used by 19 random primers. Ten of nineteen primers showed polymorphism patterns. Statistical analysis of data was performed by Popgene software. In this study, the maximum of genetic variation was in north of the Caspian Sea. Also, the maximum of genetic distance was between north area and Golestan coasts (0.089) and the minimum of genetic distance was between Mazandaran and Guilan coasts (0.001). The UOGMA dendogram showed two clusters. The samples of Mazandaran , Guilan and Golestan coasts placed in one cluster and samples of north area in other cluster The genetic diversity was significantly different between samples of north area and Golestan coasts (p≤0.05). As result, there is a significant genetic divergence between some of samples. Therefore, two genetic group of Mnemiopsis leidyi were identified in the Caspian Sea.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Ecological ; RFLP ; RAPD ; Mnemiopsis leidyi ; Genetic variation ; Population ; Molecular Test ; Samples ; Enzymes ; Genotype
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 47pp.
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    Surveying the biology of Capoeta capoeta gracilis and it s distribution, the goal aquaculturize the species (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This Research has been done monthly in Sefidroud river and its tributaries from Agust 2003 to July 2004 . From the total number of 2027 specimens of caught fish , 800 and 629 were males and females respectively , and 598 specimens were undistinguished. Samples were caught by cast nets. Fork length range of all specimen was 33-255 mm (119.56 ±46) and their weight rang was 0.42-257.5 gr (34.4 ±39.06) gr and their age range was 0-5 (1.76 ±0.959) year. The avrege of relative frequency in C.c.gracilis was 65.1 % . The average of condition factor was 1.07±0.13 . Between Fork length and weight of C.c.gracilis . Was exponential relation and coefficient of correlation was estimated 99.11% . Growth cofficient (k) was 0.114 ; L∞ was estimated 373.5 mm. C.c.g consumed of Chrysophyta phylum more than other Phytoplankton phyla in Sefidroud river and from Bentic point of view, consumed of clay, Filamentous algae detritus, insects, seeds. Absolute fecundity range was 1823-9274 (4553.8 ±2169.1) eggs. Considering the GSI and frequency percent of maturity stages, spawning period of the fish begins from April and peak of spawning is in June and terminates in July.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biology ; Survey ; Capoeta capoeta gracilis ; Aquaculturize ; Species ; Specimens ; Males ; Females ; Fish ; Samples ; Weight ; C.c.gracilis ; Growth ; Chrysophyta phylum ; Phytoplankton phyla ; Filamentous algae detritus ; GSI ; Maturity ; Spawning
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 96pp.
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    Isolation and molecular identification of probiotic bacteria from Cultured shrimps Litopenaeus vannamei in Bushehr province (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Shrimp culture and rearing industry in particular white leg shrimp species (Litopenaeus vannamei) is one of the major activities in the world, including Iran. The outbreaks of diseases are the main factors limiting production. Due to the adverse effects of abusing antibiotics and chemical disinfectants, disease control and prevention requires novel methods that are affordable, effective and safe for the environment and humans. For this purpose during the five months, sampling was done from three main sites of shrimp culture in Bushehr province and 150 pieces cultured white leg shrimp, 135 water and sediment samples were collected from the ponds, input and output channels by standard methods. Physicochemical parameters of water and biometry of shrimps were done and recorded. Probiotic isolation was performed by culturing samples in Tryptic soy agar and TCBS after incubation in 30º C for 24-48 h. The antimicrobial effects of culture extract of isolates were evaluated against V. harveyi with the well diffusion method and the best isolates were selected. The molecular identification of selected bacteria was performed by 16S ribosomal DNA gene sequence analysis technique. The growth kinetics of selected bacteria and effect of environmental factors on antimicrobial compound production and stability of them in salinity (1.5-5.5%) and (0-50ppt), pH (5-9) and temperature (3040º C) and (35-100º C) were studied respectively. After the determination of antibiotic resistance profiles of them, the In vivo test on the L. vannamei post larvae was performed and effect of these bacteria on growth performance, prevention of vibriosis and water quality include (ammonia, nitrate nitrite, turbidity, total bacteria and Vibrionaceae load were examined. For extraction and identification of bioactive material with anti-bacterial properties produced by selected bacteria, dialysis, SDS-PAGE electrophoresis, ultraviolet spectroscopy and gas chromatography mass was performed. According to the results of this dissertation the most frequency of facultative aerobic and anaerobic heterotrophic bacteria was seen in shrimp digestion tract (3.04 ± 0.75) ×105 CFU/g in September .Genus Vibrio spp. (37.88%) and Bacillus spp. (27.27%) had the most frequency respectively. Of the 198 bacterial isolates, two bacterial strains from the digestive tract and sediment which had the highest inhibition and a stability antagonistic effect on V. harveyi were selected. Based on 16S ribosomal DNA gene sequence analysis, they were identified as Bacillus subtilis subsp. inaquosorum strain IS02 (GenBank: JN856456.1) and Bacillus vallismotis IS03 (GenBank: JQ085958.1) and recorded in the National Center for Biotechnology Information (NCBI). According to results of identifying bioactive material produced by selected bacteria, in SDS-PAGE electrophoresis Bacillus subtilis subsp. inaquosorum strain IS02 did not have any band but Bacillus vallismotis IS03 had two band in 25 kDa and 34-45 kDa molecular weight which probably bacteriocin like substances and group III Bacillus bacteriocin respectively. UV spectra of selected bacteria were in the range (260-265nm) which related to peptide groups. In GC-MS the highest percent of chromatogram was belonged to pyrrolopyrazines in both of selected bacteria, which have antibacterial properties. Overall, according to results both of the selected bacteria were adapted to the ecological conditions of shrimp culture and could be used as appropriate probiotics in this industry. It is hoped that the results of this research is to produce a step towards the realization of national production of indigenous probiotic in Iran.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Chemical ; Environmental ; Isolation ; Molecular ; Probiotic Bacteria ; White leg Shrimps ; Litopanaeus vannamei ; Shrimp ; Culture ; Species ; Sediment ; L. vannamei ; Genus Vibrio spp. ; Bacillus spp. ; V. harveyi
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 237pp.
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    Sexual diversity of red algae using isomorphic generations of Gracilaria corticata in the northern Persian Gulf and Oman Sea (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: In conformity with the sex determination of Gracilaria corticata in the Persian Gulf and Oman Sea, a total of 41 samples were collected from two stations of Bostaneh region (northern Persian Gulf, 54° 38´ E / 26° 30´ N) and Lipar regions (northern Oman Sea, 60° 49´ E / 25° 15´ N). The specimens were cultured in PES media for observing the different life stages. The anatomical structures of thallus were taken into consideration. The diploid tetrasporophytes and spermatangia in thallus of male’s gametophytes; and Carpospore and cystocarps of female were determined. Due to DNA extraction, the parasites and epiphytes were cleaned and then the under growing sections were sectioned using liquid nitrogen. After extraction of DNA, by using 20 different primers according to ISSR molecular indicator, the sex diversity and genetic diversity of populations were studied; and four primers were selected ultimately . The obtained results were analyzed by GenAlex and PopGen softwares. In total, 74 bands, all polymorphisms, were propagated. According to PIC index, polymorphism separation of primer C (0.33) was higher than other primers. The Marker Index was measured between 4.48 and 6.51 with mean Shannon’s index of 0.46. The genetic similarity amongst algae was 96%. The genetic diversity inter and intra populations had significant differences of which 83% of total diversity was related to the intra diversity and 17% was related to inter diversity populations. The highest genetic distance belonged to the specimens 5 (Bostaneh) and 35 (Lipar), and it indicated the inter populations diversity in addition to intra population. As an overall conclusion, these populations can be considered as broodstocks for hybrid production for further species breeding and also to attain the maximum heterosis in adaptation with environment. In Ward clustering analysis, the dendrograms showed 5 different clusters in genetic distance of 12.18 of isomorphic phases. The PCA analysis as a complementally method was used for attest the findings. In this research, the ISSR primers could determine the male and female gametophytes and diploid tetrasporophytes in which the primer A (bands of 1200 & 1700 bp) specific for diploid tetrasporophyte and band of 300 bp specific for male were produced. The primer C showed the bands of 820 & 900 bp for diploid tetrasporophyte, and 500 bp for female gametophyte. The primer AB (990 bp) for male, 520 bp for female and 1600 & 1900 bp for diploid tetrasporophyte were specified. The primer ABC showed the specific band of 1100 bp for male; 500 bp for female; and 1200 & 1500 bp for diploid tetrasporophytes.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Sexual ; Diversity ; Red algae ; Generation ; Gracilaria corticata ; Samples ; DNA ; Population ; Broodstocks ; Environment ; Gametophyte ; Tetrasporophyte ; ISSR
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 108pp.
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    Trout Farms and Other Human activities Effects on Cheshmehkileh River Ecosystem in Tonekabon (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Cheshmehkileh River and adjacent mountainous streams, play a strategic role as a historical axis for anthropogenic civilization, human welfare also habitat and migration pathway of commercial – biologic valuable fishes e.g. Caspian trout, Caspian kuttum, members of Cyprinidae family in south Caspian Sea drainage. Treats such as overfishing of Caspian trout and Red spotted trout stocks in mountainous headwaters, barriers construction and manipulations those are out of river carrying capacity developed by human activities, affected normal function of river as well. Sand mining big factories establishment next to the river, legal and illegal trade of river sediments, direct entry of Tonekabon landfill leakage into the river, development of Rainbow trout farms since 3 decades and huge effluents into the river containing dead fish and types of solids, escapement of cultured Rainbow trouts into the river, … are major minimum factors which needs basic information for integrating inclusively drainage management system. Cheshmehkileh River contains Headwaters of Dohezar (Daryasar & Nusha), Sehezar and Valamroud rivers during 13 monthly sampling phases between September 2009 and October 2010 based on macrozoobenthoses investigations by EPT, EPT/C EPA protocols, measurements of nominated physic-chemical and microbiologic parameters. Probability of Rainbow trouts escapement and invasion, existence, nutrition in Cheshmehkileh environment indeed investigated. Data analysis explained significant differences (P〈0.05) between groups of measured parameters in different sampling stations. Dendogram of clustered analysis based on consolidation of major biologic/ physic-chemical and microbiologic parameters, separated stations No. 1, 3, 2, 4 in one group and remained classified in different groups. Station 8 and 9 similarly separated which expressed general similarities according to Sehezar river environment which were differs in comparison with other stations. Station 11 separated according to its natural quality of water and environment. Similarities between station 10 to Sehezar river stations 8 and 9 expressed general influence of Sehezar River more than Dohezar River in Cheshmehkileh condition especially in station No. 10. High scores of EPT and EPT/C indices in upstream stations 1, 3 and 8 also low score of indices in stations 7, 13 and 6 expressed levels of environment quality between these groups of stations. Maximum average biomass of macroinvertebrates belongs to Trichoptera order in Cheshmehkileh River. Significant decrease of biomass in stations 11, 12 and 13 in comparison with other stations stated environment degradation in mentioned stations relevant to excessive sand mining as well. Pollution resistant groups of invertebrates significantly increased in downstreams against upstream stations. Also disappearing of Plecoptera order in station No. 7, 9, 10 and 13 stated low quality of environment in comparison with upstream stations. Confirmation of effects quality and quantity for point and non-point sources of imported pollutants require specific management considerations in order to present exploitations, pollutants control and emergencies for river monitoring in forthcoming years.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: River ; Pollution ; Aquaculture ; EPTC ; Assessment ; Macroinvertebrates ; Chemistry ; Microbiology
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 138pp.
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    Creating a sperm bank from sturgeon brood stocks in the South Caspian Sea (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Sturgeon stocks are drastically decreasing in Caspian Sea during recent decades. This project has been proposed to collect the excess sperm of Caspian Sea Southern part sturgeon breeders, to cryopreserve and hold them for further use. During two spawning seasons (2006-2007), sperm quality and quantity analysis were conducted on 34 different male sturgeons of which 12 Persian sturgeon, 4 stellate sturgeon, 1 beluga and 1 ship sturgeon with better quality were chosen to cryopreserve. After dilution of fresh sperm with sturgeon sperm diluent (115 mM Tris, 23.4 mM sucrose and 15% DMSO) at the ratio of 1:1, the 0.5 ml straws were filled using special filling and sealing apparatus and frozen with a multi step freezing rate. To access the further quality and fertilization test, samples were thawed at 40 °C for 20 seconds. During the project performance, 1010 ml of Persian sturgeon sperm , 110 ml of ship sturgeon sperm , 90 ml of stellate and 80 ml of beluga sperm were cryopreserved. The reduction of frozen-thawed sperm motility in comparison with fresh samples showed 10-52.3 % decrease, but there was not any significant differences between samples after 7 months preservation in liquid Nitrogen (P 〈 0.005). River caught Persian sturgeon samples showed higher sperm density in comparison with nonriverine breeders (P 〈 0.05). Data showed that stellate sturgeon sperm showed better resistance to freezing condition in comparison with Persian sturgeon spermatozoa. Fertilization tests (4 hours after fertilization)showed 27.4% and 58.2% fertilization rate in frozen-thawed stellate and Persian sturgeon spermatozoa, respectively.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Spermatozoa ; Cryopreservation ; Percentage of motility ; Sperm bank ; Sturgeon ; Brood stocks ; Spawning ; Fertilization ; Samples ; Density
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 50pp.
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    Investigating and determination exploitation pattern of jinga shrimp (M.affinis) in the Khouzestan province Waters (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Study of shrimp stock was carried out from September 2007 to November 2009 in west of Khuzestan coastal waters. In this period, twelve cruises in Life- Buseif (western coastal area) were carried using shrimp trawl net. In Life- Buseif fishing area, the CPUE of total shrimp was the highest (44.2 kg/ h) in 21st October 2008. In this area, the highest biomass of total shrimps was in same time (982.4 t). This value was 808.5 t for M. affinis. Maximum mean length of males and females of M.affinis were observed in 4th November 2009 (12.1 & 11.3 cm respectively). Maximum and minimum sex ratio (f:m) of this species was 1.8:1 in 15th November 2008 and 0.6:1 in 3rd November 2008. Relative frequency of shrimp in total catch was observed in November (22.2%). According to the results of this study, shrimp catch season was opened in Life- Buseif from 4th Nov until 24th Dec 2007, from 24th Oct until 14th Dec 2008 and from 27th Oct until 17th Dec 2009.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Shrimp ; CPUE ; Jinga Shrimp ; M.affinis ; Stock ; Trawl net ; Length ; Males ; Females
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 39pp.
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    Ecological study in ponds of Litopenaeus vannamei (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This study has carried out on five earth ponds of Litopenaeus vannamei shrimps investigation and Mahan Siraf farm s which located at Helleh site in the Bushehr province from may 2007 . main objectives’ were : (1) Measurements of quality factors in water and sediment, Planktons and benthoses, (2) quality factors’ effects on growth and survival of Litopenaeus vannamei. With respect the time of shrimp stocking and harvesting, the frequencies of water and sediment sampling were done per 10 day and once per month, respectively. both sediment sampling of the pond beds have done before and after harvesting. The measured factors in the water ponds were : pH, salinity, dissolved oxygen, transparency, temperature (Twice a day in the morning and the afternoon), chlorophyll-a, nitrate,nitrite, orthophosphate, iron, biochemical oxygen demand, TSS, TDS, Planktons. The parameters (pH, total organic matter, iron) were measured in sediment. The measurement of grain size has done in two times (before and after harvesting). independent variables including nitrate, Ammonia, orthophosphate, iron and chlorophyll-a had significant correlations with shrimp weights as dependent variable. Nitrate showed the highest correlation with the shrimp weights (r = 0.921). overall correlation of fifth most important factors with the shrimp weights, namely nitrate, orthophosphate, Ammonia, iron and chlorophyll-a was estimated to r=0.999. The results show low concentration of the essential nutrient such as phosphate and nitrate, high concentration of ammonia, undesirable’s transparency, and a great amount of clay in pond beds . On the basis of above description, required to renewing management in fertilizing, feedings, liming qualities of the bed.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Ecological ; Litopenaeus vannamei ; Investigation ; Shrimp ; Salinity ; Ponds
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 86pp.
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  • 77
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    Biomass estimation, opening and closure of Shrimp (Penaeus semisulcatus) fishery in Bushehr waters (Persian Gulf) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: From June 2004 to March 2006, a survey in Iran waters (Bushehr province) has completed. Sampling was accomplished in two area, north and south. In this period 15 cruises achieved in summer and 3 cruises in autumn and winter. In each cruise of north and south area 18 and 30 stations were sampled respectively. Stations row on three depth stratums that is, below 10 m, 10-20 m, and 20-30 m in total area from Bahrekan to Motaf. Some Biological parameters such as sex stages, sex ratio, mean total length, species composition were measured. Duration of shrimp fishing season in years 2004, 2005 and 2006 were 40, 44 and 40 days in summer respectively. In June and august the Catch per Unit Effort (CPUE) in south was more than northern area. CPUE increases in north area especially at the end of summer during fishing season gradually. Computing on Biomass estimation resulted 674, 1024 and 873 ton in years 2004, 2005 and 2006 respectively for all shrimp species (usually tiger shrimp, white shrimp and other). Total commercial landing of shrimp catch in above years were 1602, 1597 and 1110 ton respectively. Percentage of tiger shrimp in commercial landing in above years was 77, 75 and 84 respectively. We found that the least mean length of tiger shrimp was observed in southern area especially from Motaf to Rodmond stations. The schools of prawn penaeus semisulcatus enter to Bushehr waters from southern area that is Motaf region. Other than Motaf; Nakhilu, Rase khan and Rod-mond show same characteristics. Also in this region, density of shrimp increases with depth in most summer cruises. The result from analyses of gonad maturation studies shows the adult and mature females (stages III and IV) of P. semisulcatus accumulate in northern area at the end of autumn. This stock catches in autumn by illegal fishing.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biological ; Biomass ; Shrimp ; Penaeus semisulcatus ; Fishery ; Green tiger prawn ; Opening season ; Closure season ; Survey ; Sampling ; Sex ratio ; Species ; CPUE ; Illegal fishing ; Parameters
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 73pp.
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  • 78
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    Investigation on feasibility polyculture of Litopenaeus vannamei with Mugil cephalus in earthen shrimp ponds (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This study was conducted in order to optimizing of the biological condition and enhancing of the harvest efficiency for cultured shrimps of Guater site. Experimental design was consisted of three treatments and each treatment with three replications (totally 9 ponds with 600 m2 area for each pond). After preparing and watering of ponds, the ponds was stocked with shrimp post larves in a density of 0.007±0.001 g (or 20 numbers per m2) per m2. After 35 days, the fingerlings of gray mullet were released to shrimp ponds in densities of 0/100 m2 (T1/), 2/100 m2 (T2) and 4/100 m2 (T3). During experiment, the physicochemical parameters of water (temperature, O2, pH, water transparency and salinity) were measured daily for two times (morning and afternoon). Also, the nitrate, nitrite, ammonia, phosphate and silicate concentrations and BOD5 as well as chlorophyll (a), phytoplanktons and zooplanktons were measured every 15 days one time. To determine the organic values of the bottom sediment of the ponds, monthly sampling was conducted. Health status of shrimps was investigated monthly too. Growth rate, mean weight, survival value, Food conversion ratios (FCR) and total shrimp harvest (Kg) were measured after 107 days rearing in ponds. ANOVA analysis showed significant differences between treatments. The highest (212 Kg, 3533 Kg; weight mean was18.4 g for each shrimp) and lowest (187 Kg, 3116 Kg; weight mean was16.23 g for each shrimp) of harvested shrimps were yielded in T3 and T1 respectively. There was not significant differences between treatments in terms of survival rate (P〉0.05). After 107 days rearing, the highest (1.27 0.2) and lowest (1.2 0.1) of FCR were observed in T3 and T1 respectively. Although, there were no significant differences between T2 and T3 in ammonia, O2, Total organic material, pH and BOD5, but, T2 and T3 had significant differences with T1 in these parameters. In this study, 27 genus of phytoplanktons belonged to Diatoms (Bacillariophyceae), blue-green algal (Cyanophyceae) and dinoflagellates (Dinophyceae) were identified. Diatoms with 20 genus had more abundance than dinoflagellates (6 genus) and blue-green algal (1 genus). Also, 7 orders of zooplanktons including copepod, mollusks, nauplius of crustacean and rhizopoda were identified. Health investigation of T1 (without shrimp) showed more pathogenic pollution (parasites and bacteria) than in treatments with shrimp. In this regard, among bacteria, the Vibrio genus (V.Alginoliticus and V.Fluvialis) had more abundance and among parasites zoothamnium sp and Epistylis sp were identified which more abundance was for zoothamnium sp. In conclusion, our results concluded that mixed culturing of white shrimp and grey mullet in optimum densities is possible and is caused more production of shrimp.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biological ; Physicochemical ; Experimental ; Litopenaeus vannamei ; Mugil cephalus ; Mixed culturing ; Polyculture ; Earthen ponds ; Shrimp ; Fingerlings ; Gray mullet ; Temperature ; O2 ; pH ; Water transparency ; Salinity ; Chlorophyll a ; Phytoplanktons ; Zooplanktons ; ANOVA ; Diatoms ; Bacillariophyceae ; Blue-green algae ; Cyanophyceae ; Copepod ; Investigation
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 92pp.
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  • 79
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    Extraction of chitin from crustaceans, shrimp, crab, lobster (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Sea is one the great resources of food stuffs, chemicals and industrial materials, shrimp, crab, lobster because of good taste and lots of protein and minerals are very worthy.....
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Chitin ; Shrimp ; Crabs ; Lobster ; Chemical
    Repository Name: AquaDocs
    Type: Report , Not Known
    Format: 83pp.
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  • 80
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    Study on P.semisulcatus and M.affinis distribution in the Bahrkansar region (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: One of the most difficult problems in fisheries resource management is to adjust for the fishing season of a particular species to avoid overlapping with reproduction season of another. Penaeus semisulcatus reproduces in winter and is harvested in August, but Metepenaeus affinis demonstrates an opposite behavior in North-west of Persian Gulf. In such a condition catch season of one species may harm the resources of the other species, In order to preserve the resources of P. semisulcatus in fishing season of M.affinis, a survey on distribution of these two species was carried out by investigating the behavioral differences in the Bahrkansar region. The project was carried out in region between Gnaveh to Khoore- Mossa waters in North-west of Persian Gulf from Jan to March 2002, a total of 51 stations were sampled in a depth range of 10 - 30 meters using a bottom trawl net. Three shrimp species, namely P.semisulcatus, Parapenause stylifera and Carssicornis solenacera were caught. In all stations M.affinis was absent. According to cited references, the depths of less than 10 Ineters in the region is considered to be the habitat of M.qffinis. While occunence of P. semisulcatus was found to be rare. According to this study, unlike for M.affinis, the survey area is a suitable habitat for P. semisulcatus in winter. Therefore cosure of shrimp fishery in depths of 10-30 meters mey help protecting the resources of P. semisulcatuse.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: P.semisulcatus ; M.affinis ; Distribution ; Shrimp ; Fishery
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 43pp.
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  • 81
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    A study on the status of shrimp diseases in Iran and whole the world and to present the best practice management in shrimp farms (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Shrimp culture in Chabahar in the Sistan and Belochestan province is one of the most important potential for development. From 1386 the white spot syndrome virus (WSSV) observed and collapsed all activity in this area. This virus hugged mortality in the world and in Iran from 1381 in Abadan area this disease induced high mortality in shrimp culture. Different methods applied for prevention WSSV in the world and the main methods consist of used specific pathogen free post larvae, immune stimulant, biosecurity and vaccine. Biosecurity is the best method for prevention the WSSV disease and include different part such as used crab fence, low stocking density, used best quality PL, aeration in the pond and sanitation all equipment and farm. Temperatures play an important role in outbreak of disease. Because in Chabahar area the culture time is in the monsoon season and changing the temperature in this season is trigger factor for WSSV outbreak. In this research study we recommended the season’s culture changing from August to March for prevention the temperature variation in this area and applied biosecurity for control the WSSV outbreak.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Shrimp ; Culture ; WSSV
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 66pp.
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  • 82
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    Investigation on Effects of ergosan^TM and vibromax^TM in control of White spot disease (WSD) and Vibriosis in shrimp Litopenaeus vannamei (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Complementary feedstuff extract from Laminaria digitata and Ascophylum nodosum that containing 1% alginic acids as shrimps stimulating of immune system for control of WSSV is used in this study. The test shrimps Litopenaeus vannamei in larvae stage (Z1-PL1), post larvae stage (PL1-PL10) and juvenile (from 30th day to 40th day) fed by complimentary feedstuff comprised control group. The other condition in test and control group was the same manner. Both groups after 40 days exposed to WSSV by oral inoculation. The clinical sign and mortality recorded for 10 days. The results showed the survival rate of exposed group was higher than the control group and it was significant (p〈0/05). The result also show the mortality observed in exposed group 48 hours later than the control group. This study can led us to new methods for control of White spot disease.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Clinical ; See weed ; Laminaria digitata ; Aschophylum nodossum ; Litopenaeus vannamei ; Control ; White spot disease ; Alginic acid ; Shrimp ; Larvae ; Juvenile ; Mortality
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 32pp.
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  • 83
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    Investigation on possibility of survival and growth enhancing by selected seaweeds from Persian Gulf in post larvae shrimp Litopenaeus vannamei (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The use of antibiotics in aquaculture has been limited. Scientifics seeking for natural substitutes to prevent of aquatic animals diseases. Considering seaweeds are rich of nutritions and bioactive compounds, the propose of this study is: investigation the potential and use possibility of native seaweeds from Persian Gulf in shrimp aquculture industry to improve growth, survival of postlarvae and to resistance against pathogens such as vibriosis. For this propose 7 macroalgaes species from Bushehr province coast, inclouding: green algae (C.iyengarii), brown algae (S.angutifolium and S.ilicifolium) and red algae (L.snyderiae, K.alvarezii and G.corticata) were collected and identified. Then seaweed extracts abtained by Water, Ethanol, Methanol and Chloroform solvents by soaking method. In vitro antibacterial activity of extracts against Gr+ bacteria (S.aureus and B.subtilis) and Gr- bacteria (V.harveyi,V.alginolyticus and E.coli) was conducted by Agar diffusion, MIC and MBC methods. Antioxidant activity also by DPPH and EC50 methods was investigated. According to results of these two tests four seaweeds species (S.angutifolium, L.snyderiae, K.alvarezii and G.corticata) were selected for use in shrimp postlarvae (PL22) diets by Bio-Encapsulation (Artemia enrichment). Before of enrichment, toxicity effect of extracts to Artemia naplii were evaluated by determination of LC50 24 h method. From results of this section Ethanol extracts were selected to bioencapsulation. After encapsulation shrimp postlarvae divided to 12 groups in triplicate, namely: C-, C+, S (200), S (400), S (600), L(200), L(400), L(600), G(300), G(600), K(300) and K(600). During 30 days of reared period C- and C+ use of basal diet and unenriched Artemia, but the other groups use of basal diet and enriched Artemia. Except C-, the shrimps in first day of culture put in 107 cfu/ml v.harveyi suspension for 30 minutes, and after water exchange 10 ml of this dose was added to reared aquaria. After 30 days survival percentage, obtained weight and SGR% were investigated. To evaluate vibrio loadind, every 10 days 5 postlarvae were sampled randomly for vibrio count. Results showed that vibrio count in C- was less than the others and in C+ was more than the others. In treatments vibrio count in L(200) was the most and L(600) was the less. Survival rate in C- was the most and after that G(600) with 79.4±6.6% and then S(300) and K(600) were 73.3±7.3% and 70.6±6.6% respectively that were significantly compare the other (P 〈 0.01). Also the C+ was the less with 33.3±6.6% that difference was significant (P〈 0.01). In this study growth parameters of all groups that fed by enriched Artemia were better than C+ (P〈0.05). After cultre period 10 shrimp of every aquarium disinfected and reared for 10 days like before treatment. After 10 days the shrimps were challenged by 3×108 cfu/ml V.harveyi and mortality was recorded for 7 days. The all of animals in C- were survive but more than 90% of C+ were dead. And survival in all of treatments were better the C+ (P〈0.05). the study showed the ethanol extracts of selsected seaweed from Persian Gulf is a good source for growth, Survival and disease control in shrimp larviculture.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Survival ; Growth ; Seaweeds ; Post larvae ; Shrimp ; Litopenaeus vannamei ; Antibiotics ; Aquaculture ; Diseases ; Nutrition ; Macroalgae ; C.iyengarii ; S.angutifolium ; S.ilicifolium ; S.aureus ; B.subtilis ; Artemia ; Larviculture ; Nauplii
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 98pp.
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  • 84
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    Identification of white spot syndrom virus in cultured Penaeus indicus by polymerase Chain reaction (PCR) in I.R. IRAN (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The WSD is the important viral shrimp disease in past decade. The detection of virus in each country was investigated by polymerase chain reaction for sensitivity and accurate. In this research study we collected 23 samples from shrimp suspected to WSD. The DNA from samples collected and extracted and design two kind of primer from VP24 identified in gene bank by DNAsis software. A primer also designs for House keeping gene for positive and negative samples in all examined. The results showed the gene colon for wssv is the similar with others and 97% is consistency. The product of PCR was colon in plasmid and confirmed and this plasmid used for internal control.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: White spot syndrome virus ; DNA ; Polymerase chain reaction ; WSD ; Viral ; Shrimp ; Samples ; Penaeus indicus ; Polymerase ; WSSV ; Virus
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 39pp.
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  • 85
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    Estimation of biomass and optimum of shrimp fishing season in Hormuzgan Province (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This project was designed with for objective on estimation of Biomass and optimum time for catch season of P.merguinsis and M.affinis shrimps. This investigation was carried out monthly by swept area method in coastal waters of Hormozgan province. The optimum time for fishing season in Bandar Abbas to Sirik waters in 2004,2005 and 2006 respectively was about 25/9/2004, 24/9/2005 and 25/9/2006. Total allowable catch ( T.A.C.) for P.merguiensis and M `.affinis estimated in 2004 to 2006 were 600 , 170 tonnes , 850 , 550 tonnes and 650 , 400 tonnes respectively. The densest catch was obtained for P.merguiensis ( from 58/5 to 65/5 %) and the least catch for P.indicus ( from 0/04to 1/1 % ), respectievly.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: P. merguiensis ; P.semisulcatus ; P. indicus ; M.affinis ; M.stebbingi ; P.stylifera ; Biomass ; Fishing season ; Shrimp
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 53pp.
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  • 86
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    Investigation of the influence of different level of plant protein (30, 50 and 70 percent) in Litopenaeus vannamei shrimp diet, with 38 percent protein (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The influence of different percents of plant protein (30, 50 and 70) in the diet of white leg shrimp (Litopenaeus vannamei) with 38 percent protein, in comparison with a commercial shrimp diet (Fenneropenaeus indicus) with 39 percent protein (contain20 percent plant protein) was studied. Initial weight of shrimp was 10±1 gram. The average increase of shrimp weight in the end of culture period in experimental treatment 1 (contain 30 percent plant protein and 70 percent animal protein), 5.89±0.06 gram, experimental treatment 2 (contain 50 percent plant protein and 50 percent animal protein), 6.22±0.25 gram, experimental treatment 3 (contain 70 percent plant protein and 30 percent animal protein) 6/19±0/24 gram and in the control treatment (contain 20 percent plant protein and 80 percent animal protein) 6/42±0/40 were measured. Generally in the study of influence of experimental diets and control diet on the growth rate(GR), food conversion rate (FCR), protein efficiency ratio (PER), specific growth rate (SGR), average daily gain (ADG), demonstrate that, mentioned parameters in the control treatment is better than experimental treatments. But no significant difference measured between them (p〉0/05). Also results showed that, application of diets contain 50 percent of plant protein (with 22 percent soybean meal), and 70 percent of plant protein (with 72 percent soybean meal), can decrease costs of diet preparation and therefore shrimp production.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Commercial ; Experimental ; Plant protein ; Litopenaeus vannamei ; Diets ; Growth indexes ; Shrimp ; Litopenaeus vannamei ; Fenneropenaeus indicus ; Growth Rate ; Protein ; Investigation ; Influence
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 37pp.
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  • 87
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    Survey on possibility of broodstocking from wild P. monodon in Chabahar region (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: In last decade the policy of shrimp culture development changes to an important economic and social pole in country. This industry need to research and innovation. The P. monodon is the biggest and largest shrimp (prawn) as known shrimp. This reason says that the culture of P. monodon is more benefit. This shrimp has reported from Guatr Gulf, Pasabandar and Bris costal area of Chabahar region. After catching around 100 pieces of P. monodon in 1999 by fishermen of mentioned area, we decided to write down a project to survey on possibility of broodstok for P. monodon in 2001. In this order we start trolling in Guatr Gulf, Pasabandar and Bris and studied the presence of monodon. During trolling we did not receive sufficient shrimp because of inconveniencing them by trolling net. This less number caused that we could not breed the P. monodons.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Survey ; Broodstock ; P. monodon ; Shrimp ; Culture ; Prawn ; Trolling net
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 23pp.
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  • 88
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    Knowledge acquisition and technology transfer for the mass production of specific pathogen free (SPF) shrimp, towards independent on foreign production (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: In this study we collected the adult shrimp from farm in Bouhsher province based on the pedigree information and screening by PCR for 10 shrimp pathogen as well viral diseases and transported to the shrimp research station for first quarantine. Three different families were selected and crossed them and obtained the larval and produced F1generation. The F1 shrimp had reach an average weight (33 ±2 g) and crossed together for produced F2 generation. The larval from F2 generation transferred to second quarantine and maintained in different tank. The postlarve from F2 transferred to nuclear breeding center and cultured until the adult size. During F1 and F2 period we screened the shrimp for 10 pathogen and for prevention the inbreeding and transferring the growth and survival gen we used selection breeding with 12 primers by microsatellite technique during each generation. After 3 year we produced 8 thousand shrimp SPF and the shrimp are free of important viral disease as well WSSV.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Shrimp ; Pathogen ; SPF ; Quarantine ; Breeding
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 331pp.
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  • 89
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    Detection of best stocking density of Penaeus indicus in the semi intensive system in Hormozgan Province (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This study done for detection of best stoking density Of Penaeus indicus in intensive culture with corrporatiny Abzian Bandar Co. in northern Tiab area (Hormozgan Province) In this research measuring many factors in clude: Temperature, Dissolve oxygen, salinity, pH, turbidity, biometric and hygien of condition of shrimps. The results show Dissolve oxygen level in after noon suitable but not suitable in early mornnig for growths of shrimp. Salinity levels in early cycle of culture high an in middle to end cycle of culture decreasiy becuase in creasiny of water exchange. Turbidity level of water growths of shrimp is good and Temperature with pH of water ishigh in standard level. The resultes of hygien condition show that with in creasing density percentage of infections of bacteria and fungus and also parasites in creasiny. Also resultes show growth of shrimp very down beacuse period of culture very long and middle daily growth for all treatments down for 0.9 g Also persentaye Pie survival showes that treatment 18 -.with middle 97.3% have highest survival and more economically to other treatments. In spite of stocking density increasiny but increasiny but income from sale of shrimp decreased.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Stocking ; Density ; Penaeus indicus ; Shrimp
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 69pp.
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  • 90
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    Impact assessment of master plan of "Attain to technical knowledge of specific pathogen free shrimp production and cut off to dependence on foreign products" on Bushehr city environment: A case study, Shoghab research station (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This study is a part of the national and technology master plan entitled "Attain to technical knowledge of specific pathogen free shrimp production and cut off to dependence on foreign products". The goals of this work were to assess the environmental effects of Specific Pathogen Free shrimp production complex on surrounded region in Persian Gulf (Shoghab) research station in Bushehr city. The environmental impacts were assessed in Persian Gulf (Shoghab) research station in Bushehr city by usage of simple checklist for impacts prediction, and scaling check list for assessment during Feb 2012 till Sep 2014. Flushing the effluents to the sea is the biggest concern of this project that can resulted in health impacts on aquatic and human communities that can be avoided by treatment. Low diversity in activities beside lack of environmental sensitive elements in the region leads us to use of scaling check list for impact assessment. Environmental grade of each activity can be achieved by stratification of them according to their intensity, significance and scale. We reached +320 and -198 for positive and negative points respectively that sounds a clear yes for execution choice.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Environmental risk assessment ; Shrimp ; Specific pathogen free
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 120pp.
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  • 91
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    Study on Mahshahr creeks (north of Persian Gulf) as protected area (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: In recent years MPAs (Marine Protected Areas) have been known as a flexible tool to improve fishery management as well as to preserve biodiversity in valuable coastal waters habitats. This study was conducted to selecting the sensitive and high potential area that are impacted by several environmental stress and associated habitats are being damaged in Mahshahr creeks in Northwest of Persian Gulf. Study was done in eight creeks from October 2005 to September 2006. Different biotic parameters such as phytoplanktons, zooplankton, macro zooplankton, benthic animals and fishes (trawled species) communities and also physico-chemical parameters and sediments heavy metals were investigated. Different parameters were sampled monthly or seasonal from each creek. Water samples with bottle sampler and sediment were collected by Peterson grab. Several ecological and biological indices based on heavy metals and benthic communities were used. Concentrations of heavy metals were measured by using voltammetric plarography method (Methrom 797). The range and mean concentrations obtained in mg/kg were 35.16-15.03 (27.01) for Cu, 171.41-65.57 (102.672) for Ni, 20.06-4.63 (13.22) for Co, 0.78-0.093 (0.22) for Hg, 379-65.07 (113.7) for Zn, 1.00-0.27 (0.559) for Cd and 29.72-7.09 (14.66) for Pb. To evaluate the levels of sediment contaminations, the background values of the different heavy metals were calculated and contamination factor for each metals and degree of contamination for each creek determined as well. Measured concentrations were compared with International standards. Ultimately the heavy metals contamination factors (Cf) can be arranged as follows: Hg〉 Zn〉 Cu≥ Ni〉 Pb≥ Co 〉 Cd and according to contamination degree (Cd), different creeks can be arranged as follows too: Ghannam〉 Ahmady≥ Zangy〉 Doragh≥ Darvish〉 ghazaleh〉 Patil〉 Bihad Regarding the results some elements such as Hg, Zn and Ni, are at risk level and all of the studied creeks are classified in moderate degree of pollution except Ghannam showing considerable degree of pollution. The benthic invertebrates are a well-established target in evaluations of environmental quality status. The AMBI(AZTI,s Marine Biotic Index) was developed to determine the impacts and the quality status in softbottom marine benthic communities. All creeks are characterized by muddy bottom. Macrobenthic animals, according to their sensitivity to an increasing stress gradient, classified in five ecological groups. In present study due to appearance of dominant species such as Capitella sp and nematodes (as opportunist species), diversity values was reduced. Two way ANOVAs showed only seasonal significant differences in mean abundance (P〈0.05, f=5.712, df:3,21) and Richness index values (p〈0.05, f= 4.975, df=3,21), while all of creeks showed similar biological characters based on benthic communities. According to annual, mean of AMBI (BC) all of creeks classified in ecological group III with slightly pollution, except Darvish that was placed in unpolluted category. In general, according to AMBI and BI values, The most creeks are classified in unpolluted (34%) and slightly pollution (34%) categories except Zangy, Doragh and Patil in summer and also Zangy and Bihad in winter that showed moderate to heavily pollution (32%). The results of chemical quality of sediments, Cf values, Cd values, AMBI Index and water quality and risk Indices have confirmed each other. In general, the Mahshahr creeks are classified in moderate to heavy pollution status. In addition, biological parameters of benthic communities and other biotic parameters have showndescending trend in ecological quality in all of the studied creeks. Communities. According to annual, mean of AMBI (BC) all of creeks classified in ecological group III with slightly pollution, except Darvish that was placed in unpolluted category. In general, according to AMBI and BI values, The most creeks are classified in unpolluted (34%) and slightly pollution (34%) categories except Zangy, Doragh and Patil in summer and also Zangy and Bihad in winter that showed moderate to heavily pollution(32%). The results of chemical quality of sediments, Cf values, Cd values, AMBI Index and water quality and risk Indices have confirmed each other. In general, the Mahshahr creeks are classified in moderate to heavy pollution status. In addition, biological parameters of benthic communities and other biotic parameters have showndescending trend in ecological quality in all of the studied creeks. We used of some criteria for evaluation of biotic potential or sensitivity and also to explain the ecological health level of studied creeks. Positive and negative criteria are classified in three level 1,3 and 5 for low, moderate and high degree of conservation value respectively. According to gained points the creeks Darvish, Doragh and Ghazaleh in high level and creeks Ghannam , Zangy and ahmady in low level of conservation value were classified.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Environmental ; Physico-chemical ; Ecological ; Biological ; Bottom sediments ; Heavy metals ; Contamination factor ; AMBI Index ; Risk Index ; Biodiversity ; Species ; Samples ; Benthic ; ANOVA ; Pollution
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 158pp.
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  • 92
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    Investigation possibility culture of Shrimp Litopenaeus vannamei with brackish water of Caspian Sea in Mazandaran Province (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Whiteleg shrimp (Litopenaeus Vannamei) is one of the most important species for aquaculture development, because of tolerance and adaptation to different ecological conditions.The aim of this study was determine the bionormative parameters of vannamei shrimp culture using Caspian Sea brakish water in Mazandaran province and also possibility of introduction a new aquatic spciese for rereaing in Mazandaran province. The Shrimp vannamei reared with the brackish water (10±0.43ppt) of the Caspian Sea in Mazandaran province and the Caspian Sea Ecological Research Iinstitute in summer 1391, 1393 and 1394. In this study, were evaluated the effect of different densities (45/m2, 50/m2, 55/m2 and 60/m2) primary stock whiteleg shrimp postlarvae (PL12) on the growth, survival rate and also population structure of phytoplankton in shrimp vannamei. Initially, post larvae in brackish water were gradually adopted. The density of 35 per square pieces in an earthen pond (1000m2) was stocking.The rearing time was 85 days. Average daily growths: 0.31±0.037g; final mean weight: 21.11±0.71; Feed conversion ratio: 0.79 and final yield: 531kg were determined. In addition the experiment of 1391, the results of Mazandaran province vannamei shrimp culture, were observed in 1393 and 1394, the average weight average daily growing and FCR % were 20-18 g and 0.68 - 0.77 respectively during 90 days culture period with harvesting of 40 PL /m2 at cement culture pond. The experiments of effect of different densities were performed separately in 1391, in 12 circular concrete pound with a sandy bed (area: 78m2) in the four treatments and three replications for each treatment. The experiments performed in a 75-day period. During the test, the water temperature was 27.4±1.79°C. The results showed that growth parameters and survival rate in experimental treatments, there was a statistically different (Duncan test, P〈0.05). Thus, with high levels of density, the amount of weight gain and survival rate (SR), specific growth rate (SGR) and daily growth rate (ADG) has decreased. In addition, the treatments were different variations of FCR (P〈0.05) and not depend on the primary stock density of postlarvae. The highest growth (SGR=11±0.04 and ADG=0.25±0.01) and survival rate and calculate the amount of production per hectare (5596±433) were observed in low density (45m2). In this study identified 20 genera of phytoplankton from five division of the Phytoplankton. Phylum Cyanophyta, with 30 percent of the identified genera that much more constituted. And the phylum Chlorophyta with 25 percent, of Bacillariophyta 20 percent Pyrrophyta 20 percent and Euglenophyta 5 percent had the lowest frequency. Chlorophyta with 25 percent allocated to the division, with 20 percent of Bacillariophyta, with 20 percent of the division; Pyrrophyta Euglenophyta division with 5 percent had the lowest frequency.Euglenophyta phylum was identified as a species. Therefore, based on acquired results, was observred that, vannamie shrimp was reared well using Caspian Sea brakish water in Mazandaran province. In addition, it seems that, vannamei shrimp was gained better results in Mazandaran province compared to other provinces, because of suitable water salinity.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Vannamei shrimp ; Brackish water ; Saline water ; Culture ; Shrimp ; Litopenaeus vannamei ; Aquaculture ; Stock density
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 58pp.
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  • 93
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    The use of modified atmosphere packaging for improvement shelf life of fresh Oncorhynchus mykiss (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: In this study, influence of modified atmosphere packaging on shelf life of trout (Oncorhynchus mykiss) (whole fish without visceral and without head and tail fish) stored in 4 to 6ºc was examined. Fish stored in MAP condition and control samples, in different time, were tested for spoilage chemical factors (TVN, PV and pH), microbial parameters (total viable count, clostridium botulinum) and sensory factors too. Mixed gases including co2 (30-50%), N2 (40-65%) and o2 (0 to 20%) were used for trout (without head and tail =6 treatments) and (whole fish without visceral and control = 2 treatments) statistical the analysis results showed that examined factors were significant difference during storage (P〈0.001). Mixed gases haven t had inhibitory effect on spoilage factors (chemical and microbial parameters). However spoilage process was delayed. Increasing of chemical and microbial changes in control samples was higher than treatment samples especially TVN. The results also showed that shelf life of control samples stored 4-6ºc were between 6-12 days but in MAP samples were 19 days. Mixed gases including CO2 (40%), N2 (55%) and O2 (5%) were the best formula and the shelf life of fish (without head and tail) was 16 days where it was 19 days in whole fish (Lack of visceral). The results showed that storage of trout in MAP condition facilities storage and increasing of fish shelf life too.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Chemical ; Oncorhynchus mykiss ; Modified Atmosphere Packaging ; Shelflife ; Rainbow trout ; Samples ; pH ; Microbial parameters ; CO2 ; N2 ; O2
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 58pp.
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  • 94
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    Role of aflatoxin B1 on hematological factors, histopathological changes, tissue residual, growth and survival rate of Fenneropenaeus indicus (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Role of aflatoxin B1 on hematological indexes, histopathological changes, tissue residual, growth and survival rate of Fenneropenaeus indicus Some similar cases of aflatoxin chronic poisoning were reported from cultured shrimps in Iran. In this study, Indian white shrimp, Fenneropenaeus indicus, with a weight of 11.79 ± 1.76 grams were fed with diets containing 0, 20, 50, 100, 200, 400, 800 and 1600 ppb levels of AFB1 for 8 weeks. Growth performances, survival rate, residue in muscles and cephalothorax, hematological and histopathological changes were determined after 4 and 8 weeks. The results showed that after 8 week of feeding shrimps with values of 20, 50 and 100 ppb aflatoxin B1, did not affect the growth rate, but with increased levels of Aflatoxin B1 in feed, the growth is reduced and the correlation (r) minus the final weight equal to - 0.312. After eight weeks doses of AFB1 in the diets showed negative correlation to the survival rate, total haemocyte count (THC), total plasma protein (TPP) equal to -0.603, -0.237 and -0.649 respectively. Histopathological changes were variety necrosis, degeneration and atrophy of epithelial and secretory cells lining the tubules, infiltration of fibroblasts in the walls of hepatopancreas tubules, separated muscle bundles in Muscle tissues and intestinal mucosal necrosis and detachment. Possible amelioration was examined by feeding control and treated shrimp (after eight weeks exposure to AFB1) by dietary without AFB1 for four weeks. The Recovery was observed in all parameters of 20, 50, 100 and 200 ppb AFB1 treatments but shrimps fed with the 1600 ppb and 800 ppb concentrations of AFB1 exhibited slower growth, more reddish discoloration disseminated over the body and lack of consistency in the hepatopancreas at 4th week recovery period. After eight weeks, the highest level of aflatoxin B1 residues in shrimp muscles was observed in 100 ppb treatment that its value was 1.55 ppb, but the highest level of aflatoxin B1 residues in cephalothorax was observed in 1600 ppb treatment that its value was 7.1 ppb. In terms of public health, these amounts of aflatoxin in shrimp body are less than permissible limit for human consumption.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Histopathological ; Fungus ; Mycotoxin ; Shrimp ; Fenneropenaeus indicus ; Tissue ; Survival
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 88pp.
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  • 95
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    Study on health and Immunity index of vaccinated Litopenaeus vannamei against white spot virus disease (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: In this study, the inhibitory effect of Radio-Vaccine against white spot disease on white shrimp (Litopenaeus vannamei) was studied. This vaccine was prepared by Nuclear Science and Technology Research Institute of Atomic Energy Organization of Iran. 14,000 L. vannamei postlarvae were used in treatments after adaptation. Eight treatments, four positive and two negative controls were considered in triplicate. Initially, three meals a day for a month in the hours of 8, 16 and 24 were feeding. Three times in day at 8, 16 and 24 clock were feeding in first mount. Waste food and fecal remaining in the tanks were removed. In the second and third months of treatment, feeding frequency increased to three and four times in day which is at 6, 18 and 24 were given concentrate feed and Artemia nauplii at 12 clocks. Vaccination was carried out by immersion. Vaccination was performed in two doses with an interval of 10 to 14 days. For biometric testing and evaluation of immune cells throughout the experiment, three samples were taken randomly from the tanks. To assess the health status of shrimp, indices of Total Hemocyte Count and Total Plasma Protein was determined. Phagocytic activity of hemocytes was evaluated to assess immune setuation of shrimps. The results of this study showed that the shrimp immune system, as in many reports, the effect of recombinant vaccines and passive safety in memory as "Alternative adaptive immune responses" after experimental infection of white spot virus (WSSV) in wild and farmed shrimp is confirmed, will respond to vaccination against white spot disease.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Immunity index ; Litopenaeus vannamei ; White spot virus ; Disease ; White shrimp ; Feeding ; Artemia nauplii ; Samples ; Hemocyte ; Plasma Protein
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 48pp.
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  • 96
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    Application of earthworm (Eisenia fetida) in diet for rainbow trout (Oncorhynchus mykiss) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This experiment was carried out to evaluate the effect of earthworm (Eisenia fetida) in diet for rainbow trout in complete random design for 6 weeks. Ten fish with mean 120 g was stocked in each replication.The earthworm at levels of 0, 25, 50, 75 and 100% was replaced (as fed) with fish meal in rainbow trout diet. The feed intake by weekly and weight gain of fishes by monthly were measured. Feed intake, weight gain, specific growth rate and feed conversion ratiothe were colculated, and the samples of fish meat were analysed for chemical composition including dry matter, crude protein, fat and ash. Results obtained showed that feed intake decreased significantly (P〈0.05) as earthworm increased. The diets contain 25% and 50% earthworm showed no significane differences compared to control for weight gain, specific growth rate and feed conversion ratio (P〉0.05). The chemical composition analysis of fish meat showed that there were no significant effects between control and 25% and 50% earthworm in diet for dry matter, crude protein and ash but, fat content decreaed as earthworm increased significantly (P〈0.05). This experiment showed that earthworm (Eisenia fetida) can be used in rainbow trout diet up to 50% instead of fish meal.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Earthworm ; Diet ; Rainbow trout ; Growth ; Body chemichal composition ; Eisenia fetida ; Fishmeal ; Weight gain ; Growth Rate ; Samples ; Oncorhynchus mykiss
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 36pp.
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  • 97
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    Quantitative and Qualitative development of shrimp culture in Iran (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Primary researches on aquaculture of endemic shrimp species carried out since 1984-5 by Iranian Fisheries Research Organization (IFRO) .After a decade, the condition for extension and development of shrimp culture in the country prepared. Despite huge investment of government for preparation and construction of shrimp sites, only small part of present potential in southern coast of the country is under production. About 18 different shrimp species inhabit in Persian Gulf and Sea of Oman, in which 4 species have larger size, and more abundant, and harvest commercially . Penaeus semisulcatus, Fenneropenaeus merguiensis and Metapenaeus affinis as commercial species distributed throughout of Persian Gulf from mouth of Arvand River in north- west to strait of Hormoz, and also some parts of Oman Sea. While Fenneropenaeus indicus distributed mainly in coastal area of Oman Sea. Shrimp culture begin in Iran by P.semisulcatus, continued by imported Penaeus monodon, and developed by using of F.indicus. After confront to slow growth and viral diseases in 2001 (Khouzestan province) and 2005 (Bushehr Province), at the same time I.F.R.O acquired the bio-technique for production of Litopenaeus vannamei, and hence the condition for introduction the new species to the shrimp culture industry provided. In accordance for development of shrimp culture, I.F.R.O carried out various research activities, and established specific research center for this purpose. Research projects regarding brood-stock management, breeding and larvae production, grow out, water management and disease identification and treatment have been carried, and induced considerable impacts on quantitative and qualitative development of shrimp culture in the country.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Commercial ; Shrimp ; Aquaculture ; Quantitative and Qualitative Development ; Culture ; Species ; Fisheries ; Abundant ; Fenneropenaeus merguiensis ; Metapenaeus affinis ; P.semisulcatus ; F.indicus ; Breeding
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 180pp.
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  • 98
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    Genetic national plan study in order to shrimp breeding and developing of shrimp culture technology (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Sampling was done using 90 post larvae which were produced by reproduction of some broodstock of Penaeus indicus in one day and reared in the same situation for 4 month. Samples were classified in 3 group high growth, medium and low (according to their weight and length). Genomic DNA was extracted from a 1cm 2 piece of muscle using the phenol-chloroform method. . The polymerase chain reaction (PCR) was done using 21 RAPD loci. And PCR products were separated on 3% Agarose gels. From 21 studied loci, 12 produced polymorphic bands. The most polymorphic band produced using OPAQ 9 and the least by OPAQ 7. According to Nei 1972, the highest distance (0.457) was between low growth group and medium and the lowest (0.091) between high growth group and medium, therefore the highest identity (0.912) was between high growth group and medium and the lowest (0.633) between low growth group and medium. Consensus neighbour-joining tree using Nei (1972 and 1978) resulted in two clades, the first including high and medium growth groups and the second low growth group, it appears that low growth group are depended on separated population of the two others. With considering of mean weight of F1(16.25±1.5), mean weight of 15 ±1.2 and mean weight of parent 31.6, response to selection (R) and heritability for growth in this species were estimated 1.2±0.2 and 0.07±0.01 respectively. In another part of this study Sequencing of specific bands and primer design were done and examining of them on the same age specimens is necessary in following.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Penaeus indicus ; High growth ; RAPD marker ; Genetic ; Shrimp ; Breeding ; Culture ; Sampling ; Broodstock ; Phenol-chloroform methods ; Population ; Species ; Specimens
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 147pp.
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  • 99
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    A Study on Environmental Pollutants (Organochlorine Pesticides (OCPs), Heavy metals, Hydrocarbons and Surfactants) in the southern part of Caspian Sea (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: At the present study, the environmental pollutants such as organochlorine pesticides (OCPs), heavy metals, hydrocarbons and surfactants were done during 22 months (Sept. 2009 through May 2011) located in southern part of Caspian Sea with longitude and latitude 48°-54° N and 36°-39° E, respectively. The aims of this study were to determine the seasonal pollutants matters in water layers and bed sediments of eight transect (24 stations) and the results are as follow: The maximum seasonal percentage range of OCPs were detected in spring water samples from 10, 50, and 50m depths such as (DDD, δ-BHC, heptachlor epoxide, endrin aldehyde),(DDD) and (aldrin, β-endosulfan) compounds about 62.5, 75 and 100%, respectively. The maximum seasonal residues fluctuation of OCPs were determined in spring water samples from 10, 50, and 50m depths such as aldrin (Babolsar station), aldrin (Tonekabon station) and heptachlor epoxide (Astara station) compounds about 5.03, 3.08 and 31.43 µg/l, respectively. The maximum percentage range of OCPs were detected in sediments samples from 10, 50, and 50m depths such as aldrin and α-BHC (winter), α-BHC (summer and winter) and aldrin (summer) compounds about 100, 75 and 87.5%, respectively. The maximum residues fluctuation of OCPs were determined in sediments samples from 10, 50, and 50m depths such as α-BHC (summer in Nushahr station), α-BHC (summer in Sefidroud station) and α-BHC (winter in Tonekabon station) and compounds about 5.96, 3.77 and 3.07 µg/l, respectively. The fluctuation and distribution of Total Petroleum Hydrocarbons (TPHs) concentration in different water layers samples were reduced from summer〉spring〉 fall 〉 winter, respectively. Also this trend occurred for bed sediments and reduced from winter 〉 summer, respectively. The mean concentrations of TPHs in water samples of all seasons, regions, depths and transects were less than maximum permissible concentration (MPC). In this research, a comparison of TPHs with EPA standards shown that the desile range organic (DRO) was close to EPA standards such as TPHs and also 95 percent of water data were less than MPC. But gasoline range organic (GRO) concentrations in all stations were less than the amount of EPA standard. A comparison of TPHs concentration in sediments shown that the concentration of all stations were less than of national research council (NRC) range except west part. The maximum annual mean concentrations of Hg and Pb elements were detected in surface station (50m) at Nushahr and Amirabad transects. The most water data of Cd, Pb and Hg elements in comparison with critical concentrations with Europe, the USA and Japan standards were less than amounts of those standards. The distribution and abundance of Cd, Pb, Hg and Ni elements in water samples were detected 98, 96, 77 and 6%, respectively less than the ISQGs (Interim marine sediment quality guidelines) standards. In sediments samples, the mean and maximum concentration of Hg element detected in winter in comparison with ISQGs standards was more. But the concentrations of Cd and Pb in sediments samples of all stations were low and less than of ISQGs standards. The maximum concentration of linear alkyl benzene sulfonate (LAS) from spring through winter in Anzali (spring), Tonekabon (summer), Anzali (fall) and Nushahr (winter) were 0.07, 0.45, 0.145 and 0.087 mg/l, respectively. The maximum concentrations of LSA were detected in spring and fall in west part and summer and winter in middle part. But the lower concentration was occurred in west of southern part of Caspian Sea. According to standards of surfactants and comparison with LAS concentration of this study were less than the critical points.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Organochlorine Pesticides (OCPs) ; Total Petroleum Hydrocarbons (TPHs) ; Heavy Metals ; Surfactants ; Environmental Pollution ; Hydrocarbons ; β-endosulfan ; Sediments ; Samples ; Alkyl Benzene Sulfonate
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 146pp.
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  • 100
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    Survey on ecological Characteristics‌of Horolazim wetland in Khozestan(2012-2013) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Rregarding the potential and capabilities of different socio-economic, ecological and recreational, Hurolazim wetland is allocated as a special place in the collection of ecosystems and environment and the study of existing ecological conditions is necessary to protect and restoring management. In this study, monthly Samples of four stations (stations north of the road , Imam Reza (1) and south of the road, Imam Reza (2) ,in the Rofayeh area and stations Tabor (3) and the Shatt Ali (4), in the Shatt Ali area) were taken during the year from April to March 2012. Samples included physical and chemical parameters , phyto and zoo planktons, benthos, fish, and fishing status. Phytoplankton samples using sampling bottles from 0.5 meters below the surface were taken and counted based on alive unit. Zooplankton samples were collected by 100 micron mesh size plankton net from a depth of 1.5-2 meters . Benthic animals and sediment were sampled by Van Vin grab(0.125 square meters). One sample for benthos and one sample for grain size analysis and percentage of organic matter were sampled from each stations. In order to estimate the monthly depleted catches, data collected from the census in place and sampling was done at the time. Three fishing area has inspected randomly once every 15 days and active fishermen and caches were recorded in the prepared forms during the day. In this way, the number of observed boats, the average boat per day, the catches of the boats and CPUE were registered and total fishing effort was calculated monthly. In this study, 194 species of phytoplankton were identified. Diatoms with 85 species, Cyanophyta with 59 species, Chlorophyta with 31 species, Euglenophyta and Dinophyta with 18 and one species were observed respectively. The highest abundance frequency of phytoplanktons with 38.96 percent were observed in Tabar station (3), Diatoms with 46% has shown the highest frequency and then Cyanophyta (34.17%), Chlorophyta (16.39%), Euglenophyta (1.6 %) and Dinophta (1.58%) were included of the total abundance respectively. In the summer colonies of Cyanophyta were in high density. A total of 35 species of zooplankton have been identified. from Rizopoda, the species of Arcellidae and Centripyxidae families, from Rotifera, the species of Brachionidae, Lecanidae, Asplanchnidae and Philodinidae families, from Cladocera ,the species of Daphnidae, Sisidae and Chydoridae families and from copepods, Cyclopoda families have been observed that included about 98 percent of identified zooplankton. Respectively, Copepoda (39.9 %), Rotifera (34.71 %), Cladocera (22.38 %) and Rizopoda (0.843 %) were dominant groups. The species Brachionus urceolaris with average density of 7.29 individual per liter, is the most abundant species that included 17 % of total abundance frequency in the wetland. The higher frequency of crustacean zooplankton such as Copepoda and Cladocera could be indicated an environment more favorable conditions and expected to be good status of food in the Horolazim wetland. Among the known benthic groups, families, Tephritidae (85%) and Chironomidae (7 %) of the order Diptera and Ostracoda (2 %) from crustacean, were the dominant macrobentic groups Among the studied stations, Station South Road Imam Reza (2), and the station Shatt Ali (4), were the highest and the lowest abundance respectively. The mean abundance of macrobenthos in different seasons showed the lowest and the highest abundance frequency in summer and winter respectively. According to diversity indices, The stations Tabar (3) was the highest and the Station South Road Imam Reza (2), was the lowest value of diversity index. The results showed the higher than 71.84 % of Silt-Clay percentage in all stations, The highest value of sediments TOM in the Station South of the Road Imam Reza(2) in the winter and the lowest in station Shat Ali(4) in summer were recorded. Based on HFBI index the stations north of the road Imam Reza (1) and the station Shatt Ali (4) in moderate status and the stations south of the road Imam Reza (2) and Tabar (3) in good status were classified. In this study, 15 species of fishes belonging to 8 genera and 3 families were identified. It was noticed that 87.4 percent of all identified species in the wetland belonged to the Cyprinidae family. The results of previous studies in 2006 and 2008 in the Iraqi part of Hawizeh wetland showed that the most common fish species belonged to Biah, Karas, Hemry and Shelej that included 89.3 percent of total fishing in the current study .Finally, It is evaluated that changes in water regime of freshwater wetlands, controlled the environmental characteristics and conditions. That means water plays a decisive role in the vital characteristics of wetlands. changes of quantity and quality of water may be associated with changing the quantity and quality of biotic fauna in wetland system. In the management and protection of a wetland system, it is necessary to adopt measures based on scientific principles and reasonable methods in order to protect water quality.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Physical ; Chemical ; Survey ; Ecological Characteristics‌ ; Samples ; Phytoplankton ; Zooplankton ; Planktons ; Benthose ; Fish ; CPUE
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 204pp.
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