Publication Date:
1997-03-28
Description:
Signal transmission by many cell surface receptors results in the activation of phosphoinositide (PI) 3-kinases that phosphorylate the 3' position of polyphosphoinositides. From a screen for mouse proteins that bind phosphoinositides, the protein GRP1was identified. GRP1 binds phosphatidylinositol-3,4,5-trisphosphate [PtdIns(3,4, 5)P3] through a pleckstrin homology (PH) domain and displays a region of high sequence similarity to the yeast Sec7 protein. The PH domain of the closely related protein cytohesin-1, which, through its Sec7 homology domain, regulates integrin beta2 and catalyzes guanine nucleotide exchange of the small guanine nucleotide-binding protein ARF1, was also found to specifically bind PtdIns(3,4,5)P3. GRP1 and cytohesin-1 appear to connect receptor-activated PI 3-kinase signaling pathways with proteins that mediate biological responses such as cell adhesion and membrane trafficking.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Klarlund, J K -- Guilherme, A -- Holik, J J -- Virbasius, J V -- Chawla, A -- Czech, M P -- DK30648/DK/NIDDK NIH HHS/ -- DK30898/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 1997 Mar 28;275(5308):1927-30.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Program in Molecular Medicine and Department of Biochemistry and Molecular Biology, University of Massachusetts Medical Center, 373 Plantation Street, Worcester, MA 01605, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9072969" target="_blank"〉PubMed〈/a〉
Keywords:
ADP-Ribosylation Factor 1
;
ADP-Ribosylation Factors
;
Adipocytes/chemistry
;
Amino Acid Sequence
;
Animals
;
Antigens, CD18/metabolism
;
Blood Proteins/*chemistry
;
Brain Chemistry
;
Cell Adhesion Molecules/chemistry/*metabolism
;
Cell Membrane/metabolism
;
Cells, Cultured
;
Cloning, Molecular
;
DNA, Complementary
;
Fungal Proteins/*chemistry
;
GTP-Binding Proteins/metabolism
;
*Guanine Nucleotide Exchange Factors
;
Humans
;
Mice
;
Molecular Sequence Data
;
Phosphatidylinositol 3-Kinases
;
Phosphatidylinositol Phosphates/*metabolism
;
*Phosphoproteins
;
Phosphorylation
;
Phosphotransferases (Alcohol Group Acceptor)/*metabolism
;
Receptors, Cytoplasmic and Nuclear/metabolism
;
Recombinant Fusion Proteins/chemistry/metabolism
;
Sequence Homology, Amino Acid
;
*Signal Transduction
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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