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  • 101
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    Entomologia experimentalis et applicata 93 (1999), S. 179-187 
    ISSN: 1570-7458
    Keywords: artificial diet ; insecticidal activity ; legume pod borer ; Lepidoptera ; Maruca vitrata ; plant lectins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The legume pod-borer Maruca vitrata (Fabricius), [Lepidoptera: Pyralidae] is a major constraint restricting increased cowpea production in tropical Africa and Asia. Since lectins are known to have insecticidal properties against several pests, a survey was undertaken to screen for the effects of 25 lectins from 15 plant families on the development of Maruca pod borer (MPB) larvae. The list included 8 galactose/N-acetylgalactosamine-, 7 mannose-, 5 complex glycan-, 2 sialic acid- and 3, N-acetylglucosamine-specific lectins. Feeding bioassays using artificial diet were carried out at 2% (w/w) topical levels. Although a total of 16 lectins had detrimental effects pertaining either to larval survival, weight, feeding inhibition, pupation, adult emergence and/or fecundity, only the Listera ovata agglutinin (LOA) (Orchidaceae) and Galanthus nivalis (Amaryllidaceae) agglutinin were effective against MPB larvae for all six parameters examined. Larval mortality and feeding inhibition caused by the most active lectin (LOA) was above 60%.
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  • 102
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    Entomologia experimentalis et applicata 91 (1999), S. 287-295 
    ISSN: 1570-7458
    Keywords: Lepidoptera ; Tortricidae ; oviposition ; host deprivation ; lightbrown apple moth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of mating, age at mating, the presence or absence of a plant leaf, and the deprivation of a suitable ovipositional substrate during when the first ovipositional bout after mating would normally take place, on the lifetime fecundity and fertility (percentage of fertile eggs laid) of female Epiphyas postvittana were investigated. Mating had a significant effect on lifetime fecundity, with mated females laying 2.5 times more eggs than virgin females. Age at mating had a significant effect on both fecundity and fertility, both declining with increasing age when the female was mated. In the presence of a leaf of C. japonica, mated females had a greater lifetime fecundity than when no leaf was present; females in the presence of a C. japonica leaf consistently laid more eggs each day during the first 4–6 days after mating than females without a leaf. When females were deprived of a suitable ovipositional substrate, for the first 22 h after mating, they were significantly less fecund over their lifetime than were control females. Finally, in no-choice tests with three plants of different acceptability to females, the fecundity of females differed in the order C. japonica 〉 Urtica ferox 〉 Tibouchina multiflora. This different fecundity appeared to be inversely related to the pubescence of the leaves, suggesting that leaf texture may be a suitable antixenotic resistance factor for crops to be protected from this insect. These results suggest that strategies whereby mating is delayed or oviposition reduced within a critical period after mating, may result in significant reductions in pest populations.
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  • 103
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    Journal of insect behavior 12 (1999), S. 199-211 
    ISSN: 1572-8889
    Keywords: Lepidoptera ; Tortricidae ; Lobesia botrana ; flight activity ; wind tunnel ; atmospheric pressure ; flight experience ; mating ; age ; olfaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We studied modulations of flight activity in European grapevine moth females (EGVM) by individual observations in a wind tunnel. The effect of different factors was analyzed: variation in atmospheric pressure prior to the experiments, time of day, first experience of flight, age, mating, and odor of tansy, which attracts females. The circadian flight activity showed a peak the hour preceding the onset of scotophase and sustained activity occurred during the 6 h around this peak. Females with a flight experience in the tunnel took off more quickly than naive ones (3.9 ± 7.4 vs 20.3 ± 22.8 s). Three-day-old unmated females subjected to negative variations of atmospheric pressure (10 hPa) during the 4 h prior to the experiments increased their duration of flight (12.1 ± 8.7 vs 5.3 ± 3.4 s) compared to those not subjected to variation. One-day-old females were less active than older ones; flight was shorter than in 2-day-old females (2.7 ± 6.7 vs 5.1 ± 9.5 s) and fewer of them took off (28 vs 63%). Mating also affected the flight activity of 2-day-old females; mated females flew longer than virgins (12 ± 16.8 vs 5.1 ± 9.5 s) and took off more quickly (6.5 ± 14.4 vs 19.3 ± 20.1 s). Tansy odor in the tunnel did not significantly affect the flight behavior of virgin females, but it increased the proportion of mated females that initiated flight (87 vs 70%) and duration of flight (11.2 ± 24.4 vs 7.2 ± 13.7 s), and it reduced the latency to takeoff (2.1 ± 7.4 vs 8.1 ± 19.1 s). Flight duration in tansy odor was inversely correlated with the total number of eggs laid during the female's whole life. Our experimental settings did not allow observation of movements directed toward the odor source.
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  • 104
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    Journal of comparative physiology 185 (1999), S. 131-141 
    ISSN: 1432-1351
    Keywords: Key wordsHelicoverpa zea ; Noctuidae ; Lepidoptera ; Sex pheromone ; Antagonist
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The limits of a male moth's ability to resolve closely spaced odor filaments have been investigated. Male Helicoverpa zea normally respond to their conspecific sex pheromone blend by exhibiting an upwind flight, which culminates in source contact by at least 50% of the bioassayed individuals. When loaded onto the same filter paper source containing this hitherto attractive pheromone blend, or onto a separate filter paper and co-emitted from the same pipette source with pheromone, (Z)-11-hexadecenyl acetate severely reduced upwind flight and source contact by male H. zea. A similar level of upwind flight inhibition was recorded when the antagonist (Z)-11-hexadecenyl acetate was emitted from its own point source placed 1 mm upwind of the pheromone point source, both plumes being simultaneously emitted in a continuous mode to form a confluent strand. However, (Z)-11-hexadecenyl acetate was less effective in reducing upwind flight and source contact when it was isolated and pulsed from its own source, placed 1 mm either upwind, downwind or cross-wind of a pipette source from which pheromone was simultaneously being pulsed, such that both filaments were separated in time by 0.001–0. 003 s. These results suggest that male H. zea are able to distinguish between odor sources separated by as little as 1 mm in space and 0.001 s in time.
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  • 105
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    Journal of comparative physiology 184 (1999), S. 535-541 
    ISSN: 1432-1351
    Keywords: Key words Insects ; Lepidoptera ; Macroglossum stellatarum ; Colour vision ; Red receptor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Hymenopterans have long been shown to choose colours by means of the spectral distribution and independently of the intensity (true colour vision). The same ability has only very recently been proven for two butterfly species. We present evidence for the existence of true colour vision in the European hummingbird hawkmoth, Macroglossum stellatarum. Moths were trained in dual-choice situations to spectral lights of a rewarding and an unrewarding wavelength. After training, unrewarded tests were performed during which the intensities of the lights were changed. The results confirm that the species has three spectral receptor types and uses true colour vision when learning the colour of a food source. If colour vision is not possible since only one receptor type is receiving input from both stimuli, the moths learn to associate some achromatic cue correlated to the receptor quantum catch, with the reward. The moths learn spectral cues rapidly and choose correctly after one to several rewarded visits even when trained to different colours in sequence.
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  • 106
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    Journal of insect conservation 3 (1999), S. 15-24 
    ISSN: 1572-9753
    Keywords: census ; conservation ; Lepidoptera ; population monitoring ; survey techniques
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Nature of Science, Research, Systems of Higher Education, Museum Science
    Notes: Abstract The use of light traps in sampling moth populations is an established technique used by entomologists and ecologists. However, trap data partly reflect the variable attractiveness of UV light to different species of moth. There are also potential problems of the practicality and expense of running traps in certain locations. An alternative method of recording moth populations is developed, using a modification of the transect count technique used for butterflies (Pollard and Yates, 1993) and recently applied to moths (Spalding, 1997). During transects, moths were observed by torch-light in a 5 by 5 m box, before the recorder walked on for 10 paces, and recorded moths in the next 5 m box. The transect approach was tested in the field, alongside traditional light trap and sugar methods. Transects recorded moth species for relatively little effort, produced repeatable measures of relative density, and provided habitat-specific data. This approach is likely to provide a valuable addition to light trapping in biodiversity inventories, species surveys, and in monitoring the effects of habitat management for conservation.
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  • 107
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    Journal of insect conservation 3 (1999), S. 33-42 
    ISSN: 1572-9753
    Keywords: mapping ; database ; bias recording ; monitoring ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Nature of Science, Research, Systems of Higher Education, Museum Science
    Notes: Abstract Data from the Greater Manchester Butterfly Atlas (UK) reveal a highly significant and substantial impact of visits on both species' richness and species' incidence in squares. This effect has been demonstrated for three different zones mapped at different scales. The significant impact of number of visits persists when data are amalgamated for coarser scales. The findings demonstrate that it is essential for distribution mapping projects to record data on recording effort as well as on the target organisms. Suggestions are made as to how distribution mapping may be improved, including a geographically and environmentally representative structure of permanently monitored squares and closer links between distribution mapping and the Butterfly Monitoring Scheme (BMS), which primarily monitors changes in butterfly populations. The benefit to conservation will be data that can be better used to analyse the reasons for changes in ranges and distributions, fundamental for determining priorities and policy decisions.
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  • 108
    ISSN: 1572-977X
    Keywords: Avicennia alba ; growth ; internodal sequences ; mangrove age determination ; Rhizophora apiculata ; Sonneratia caseolaris ; Thailand
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The occurrence of periodic changes in the length of the internodes of the seedlings of SE Asian mangrove species (Rhizophora apiculata, Avicennia alba, and Sonneratia caseolaris) growing in the Pak Phanang estuary (Nakhon Si Thammarat, SE Thailand) was confirmed. The annual nature of these periodic changes was verified through comparisons with the observed average number of internodes produced by plants tagged for one year. The cycles in the sequence of internodal length of the seedlings had an average (± SE) period of 16.0 ± 0.78 internodes for Avicennia alba, 25.0 ± 1.2 internodes for Sonneratia caseolaris, and 8.29 ± 0.40 internodes for Rhizophora apiculata. These values are remarkably close to, and not significantly different (t‐test, P 〉 0.05) from the average (± SE) annual number of internodes of 17.6 ± 0.8 for Avicennia alba, 28.8 ± 2.1 for Sonneratia caseolaris, and 8.03 ± 0.36 for Rhizophora apiculata produced in a year by tagged plants. In addition, the average (± SE) growth of the tagged plants was 0.39 ± 0.018 cm day-1 for Avicennia alba, 0.24 ± 0.015 cm day-1 for Sonneratia caseolaris, and 0.091 ± 0.0036 cm day-1 for Rhizophora apiculata. These estimates were very close to those (0.45 ± 0.06 cm day-1 for Avicennia alba, 0.32 ± 0.01 cm day-1 for Sonneratia caseolaris, and 0.13 ± 0.005 cm day-1 for Rhizophora apiculata) derived as the product of the mean number of internodes produced per day, calculated from the inferred number of internodes produced per year, and the mean length of the internodes of the plants. These findings indicate that the use of regular, annual changes in the length of the internodes of mangrove seedlings can be used reliably to determine their age and to estimate their growth rate.
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  • 109
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    Oecologia 119 (1999), S. 565-571 
    ISSN: 1432-1939
    Keywords: Key wordsVespula ; Lepidoptera ; Phenology ; Shared predator ; Ecological impact
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Introduced social wasps (Vespula vulgaris) reach high densities in some New Zealand beech forests, because honeydew provides an abundant high-energy food source. We manipulated wasp density to estimate an “ecological damage threshold” for large, free-living Lepidoptera larvae. There will be a continuum of ecological damage thresholds for wasp density depending on the prey species or habitat. Experimentally placed small caterpillars had a significantly higher survival rate than large caterpillars, and the survival rate of both groups decreased with increasing wasp density. Spring-occurring caterpillars have a probability of surviving of 0.90–0.95, assuming wasps are the only source of mortality. However, at the peak of the wasp season we predict caterpillars would have virtually no chance (probability of 10−78 to 10−40) of surviving to adults. Wasp abundance must be reduced by at least 88% to conserve the more vulnerable species of free-living caterpillars at wasp densities similar to those observed in our study sites. This equates to a damage threshold of 2.7 wasps per Malaise trap per day. It was exceeded for about 5 months of the year in non-poisoned sites. There are currently no biological or chemical control techniques available in New Zealand that will reduce wasp abundance below this damage threshold throughout the year. Our models show that most Lepidoptera with spring caterpillars will be able to persist, but species with caterpillars occurring in the peak wasp season will be eliminated.
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  • 110
    ISSN: 1573-143X
    Keywords: bighead carp (Aristichthys nobilis) ; growth ; Laguna Lake ; salinity tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Bighead carp (Aristichthys nobilis Oshima) fry of various ages (11, 18, and 35 days post-hatch) were exposed to the low salinities encountered during the annual intrusion of seawater in Laguna Lake, Philippines. Practical indices of salinity tolerance assessed the effect of a 96 h direct exposure to low salinities (0–16‰). Mean (MST) and median survival times (MST50) of fry decreased as salinity of rearing medium increased. Younger fry were less able to tolerate exposure to these salinities than their older cohorts. Median lethal salinity after 96 h (MLS) revealed higher tolerance among 35–day old fry (7.6‰) than 11 (2.3‰) and 18–day old fry (6.0‰), demonstrating that survival in saline water depends on their age at initial exposure to low salinities. Mean body weight of 18–day old fry reared in 0 and 2‰ for 3 and 4 weeks was higher than for those reared in 4 and 6‰ for the same period. Growth over these periods was inversely related with the range of salinities tested. These results demonstrate that, despite their known stenohalinity, bighead carp fry possess some degree of osmoregulatory capability, allowing them to survive and grow in lakes subjected periodically to saltwater inflow.
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  • 111
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    Aquaculture international 7 (1999), S. 287-293 
    ISSN: 1573-143X
    Keywords: bone mineralization ; calcium requirement ; feed efficiency ; growth ; semi-purified diet ; tiger puffer (Takifugu rubripes)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Juvenile tiger puffer (Takifugu rubripes) were fed semi-purified diets with graded levels (0–0.40%) of calcium (Ca) supplements for 8 weeks at a temperature of 23.0–25.5 °C. At the end of the feeding experiment, average final body weight increased with increasing dietary Ca levels. A diet without Ca supplement resulted in poor average final body weight and 0.05% dietary Ca supplement could not significantly improve the growth. However, supplemental Ca levels of 0.10–0.40% significantly increased growth. Dietary Ca supplements of 0.10% or more also improved the feed efficiency in tiger puffer. Ca and phosphorus contents of the bone were independent of dietary Ca supplements. However, 0.40% Ca in the diet decreased the zinc and manganese contents of bone. Results indicated that supplemental Ca levels of 0.10–0.20% in a semi-purified diet were required to maintain normal growth, feed utilization and bone mineralization of tiger puffer.
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  • 112
    ISSN: 1573-4919
    Keywords: NADH oxidase ; protein disulfide-thiol interchange ; dipyridyl-dithio substrates ; plasma membrane ; auxin ; 2,4-dichlorophenoxyacetic acid ; growth ; plant (Glycine max)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Dipyridyl-dithio substrates were cleaved by isolated vesicles of plasma membranes prepared from etiolated hypocotyls of soybean. The cleavage was stimulated by auxins at physiological concentrations. The substrates utilized were principally 2,2′-dithiodippyrine (DTP) and 6,6′-dithiodinicotinic acid (DTNA). The DTP generated 2 moles of 2-pyridinethione whereas the 6,6′-dithiodinicotinic acid generated 2 moles of 6-nicotinylthionine. Both products absorbed at 340 nm. The auxin herbicide, 2,4-dichlorophenoxyacetic acid (2,4-D) stimulated the activity approximately 2-fold to a maximum at about 10 μM. Concentrations of 2,4-D greater than 100 μM inhibited the activity. Indole-3-acetic acid stimulated the activity as well. The growth-inactive auxin, 2,3-dichlorophenoxyacetic acid (2,3-D), was without effect. DTNA cleavage correlated with oxidation of NADH and reduction of protein disulfide bonds reported earlier in terms of location at the external plasma membrane surface, absolute specific activity, pH dependence and auxin specificity. The dipyridyl-dithio substrates provide, for the first time, a direct measure of the disulfide-thiol interchange activity of the protein previously measured only indirectly as an auxin-dependent ability of isolated plasma membrane vesicles to restore activity to scrambled and inactive RNase.
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  • 113
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Lymantriidae ; white-spotted tussock moth ; Orgyia thyellina ; (Z)-6-heneicosen-11-one ; (Z)-6-heneicosen-9-one ; (Z)-6,(E)-8-heneicosadien-11-one ; sex pheromone ; synergism ; quarantine insect ; international trade ; eradication ; Bacillus thuringiensis ; microbial insecticide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In 1996, the exotic white-spotted tussock moth (WSTM), Orgyia thyellina (Lepidoptera: Lymantriidae), was discovered in Auckland, New Zealand. Because establishment of WSTM would threaten New Zealand's orchard industry and international trade, eradication of WSTM with microbial insecticide was initiated. To monitor and complement eradication of WSTM by capture of male moths in pheromone-baited traps, pheromone components of female WSTM needed to be identified. Coupled gas chromatographic–electroantennographic detection analysis of pheromone gland extract revealed several compounds that elicited responses from male moth antennae. Mass spectra of the two most EAD-active compounds suggested, and comparative GC-MS of authentic standards confirmed, that they were (Z)-6-heneicosen-11-one (Z6–11-one) and (Z)-6-heneicosen-9-one, the latter termed here “thyellinone.” In field experiments in Japan, Z6–11-one plus thyellinone at a 100:5 ratio attracted WSTM males, whereas either ketone alone failed to attract a single male moth. Addition of further candidate pheromone components did not enhance attractiveness of the binary blend. Through the 1997–1998 summer, 45,000 commercial trap lures baited with 2000 μg of Z6–11-one and 100 μg of thyellinone were deployed in Auckland towards eradication of the residual WSTM population.
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  • 114
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    Journal of chemical ecology 25 (1999), S. 1455-1479 
    ISSN: 1573-1561
    Keywords: Turnera ulmifolia ; Euptoieta hegesia ; cyanogenesis ; Anolis sagrei ; tritrophic interactions ; host range ; sequestration ; growth ; development ; oviposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We examine the effects of the cyanogenesis polymorphism in Turnera ulmifolia on larvae, pupae, and adults of Euptoieta hegesia, the most damaging herbivore of T. ulmifolia in terms of tissue loss per unit time. We provide evidence that female E. hegesia do not show preference for host plants on the basis of their cyanogenesis level but do prefer T. ulmifolia over equally cyanogenic, closely related secondary host-plant species (Passiflora sp.). Similarly, cyanogenesis in T. ulmifolia has little effect on the food preference, growth, or development of the larvae. The potential host range of E. hegesia is limited, even within the genus Turnera, but this does not appear to be due to host-plant cyanogenesis. Pupae suffer very high mortality levels in the wild that are not associated with host-plant cyanogenesis, although our studies indicate that larvae are capable of sequestering cyanogenic glycosides from their host plants and possibly of synthesizing these or similar compounds. We provide evidence that the presence of sequestered cyanogenic compounds in the larvae protects them from terrestrial-based predators such as Anolis lizards
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  • 115
    ISSN: 1573-3017
    Keywords: Tubifex tubifex ; sediment bioassay ; hormesis ; growth ; reproduction ; egestion rates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Toxicity assessment based on sediment chronic bioassays with the aquatic worm Tubifex tubifex was performed at ten contaminated sites in the industrial area of Bilbao (Northern Spain). One control and three reference sites were also included. Tubifex bioassay measures both survival and reproduction impairment. These endpoints have been contrasted and discussed in relation to somatic growth and both individual and total biomass of cocoons. Survival was only affected at one site which was heavily contaminated by organic compounds, mainly PAHs. A group of four severely ecotoxic sediments was characterised by a drastic reduction in number and size of cocoons, and adult somatic growth. In other group of sediments, some significant increases were found for these variables. It is suggested that these increases represent an effect of hormesis. An index of reproductive effort was used to integrate the relationship between somatic growth and reproduction. Values of reproductive effort at the test sediments were lower than those at the control sediment, suggesting a conservative strategy of oligochaete worms which consisted in an investment into somatic line (growth) at the expenses of offspring. Rates of food consumption which were estimated from egestion rates, were low at the contaminated sites. This fact could be related to the low production levels found at these sites and may reflect avoidance feeding behaviour of the oligochaete worms within the sediments. At some reference sites, high production could have resulted from high nutritional quality of sediments, or to an hormetic effect due to low concentration of some chemicals. Results are discussed in relation to toxicity data from sediment three-brood bioassay with Daphnia magna Straus performed separately on the same sediments.
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  • 116
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    Behavioral ecology and sociobiology 45 (1999), S. 424-429 
    ISSN: 1432-0762
    Keywords: Key words Mate choice ; Search theory ; Costs and benefits ; Satyrinae ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A general and intuitive prediction from models of mate preference is that when the cost of searching for mates increases, individuals should become less choosy. Here, we test this prediction by comparing the mating propensity of females in two populations of the butterfly Pararge aegeria. The populations originated from southern Sweden and Madeira and due to different adult emergence patterns throughout the year, the average density of males per female is likely to be lower on Madeira. Therefore, we expected that the cost of searching should be greater on Madeira and, consequently, that the Madeiran females should be less choosy. In line with predictions, the Madeiran females mated significantly sooner after the first interaction with males than did females from southern Sweden. This difference may reflect a weaker preference for territorial males over non-territorial patrollers in the Madeiran population, because of the greater costs of searching. The Madeiran females also showed a shorter time lag between mating and the start of oviposition. We discuss this unexpected result and propose that the same mechanism could also explain this population difference, i.e. different costs of searching for suitable host plants. Both search processes are fundamental for female reproductive success and we find it plausible that they can be generalised into the same theory of optimal search behaviour.
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  • 117
    ISSN: 1573-5176
    Keywords: agar ; Gracilaria tenuistipitata ; growth ; salinity ; seawater pH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Acclimation responses of the red alga Gracilaria tenuistipitata var. liui collected on the northwest coast of Philippines were determined in laboratory setups and outdoor cultivation tanks in Haifa, Israel. Growth under laboratory conditions was influenced by all three variables studied, namely, temperature (20 or 30 °C), salinity (20, 30 or39‰) and seawater pH (6.5, 7.0, 8.0 or ≥ 9.0). In 250 mL flasks lacking pH control growth was influenced by temperature only at 20 ‰, whereas at 39 ‰, growth rates were similar at 20 or 30 °C. In 500 mL cylinders in which pH was controlled, growth rates were significantly different at a pH of 6.5 and 7.0 for all salinities, with maximal rates occurring in 39 ‰. At pH 8.0, and above, growth rates between salinities were similar and reduced to approximately 50% at a pH of 9.0 compared to rates at a pH of 6.5. Photosynthesis responses generally resembled growth responses both, in 250 mL and 500 mL cultures. In 40-L outdoor tanks, weekly growth and agar yields were apparently enhanced by increasing light intensities (up to full sunlight) and nutrient concentrations (up to 0.2 mM PO3 2- and 2.0 mM NH4 +), and rates averaged four times higher than rates determined in the smaller flask cultures. This study shows broad salinity tolerance of G. tenuistipitata var. liui and its ability to sustain growth rates that are among the highest measured for Gracilaria spp. in outdoor cultures.
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  • 118
    ISSN: 1573-5125
    Keywords: eutrophication ; feeding ; growth ; Mytilus edulis ; validation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract EMMY is an ecophysiological model of the growth and reproduction of a single mussel (Mytilus edulis L.). It contains feedback loops in the uptake and metabolism of food and in the partitioning of carbon and nitrogen to the internal state variables somatic tissue, storage, organic shell matrix and gametes. In this paper EMMY is used to simulate individual mussel growth in a series of mesocosm experiments with different inorganic nutrient loads (N and P). The experiments explore the impact of eutrophication reduction scenarios on mussel growth under defined and controlled conditions. In earlier studies EMMY was calibrated using expert knowledge on growth and reproduction during a period of 5 years. The resulting calibrated model was validated for system inputs and observations of three ecosystems with significantly different food and silt concentrations. EMMY reproduced the mussel growth sufficiently accurate in ecosystems with moderate or high food concentrations. In this study EMMY was adapted in order to cope with low food concentrations, then recalibrated (using the original calibration data and procedure) and applied without further calibration to 3 replicated mesocosm experiments. The EMMY simulations in this study show the ecophysiological response of mussels to different food (phytoplankton and detritus) concentrations. It is concluded that the mussels can adapt to significantly reduced food concentrations, due to inorganic nutrient load reduction, and still maintain growth.
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  • 119
    ISSN: 1573-0778
    Keywords: batch culture ; conditioned medium ; growth ; hybridoma ; inoculum ; protein productivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Apart from gas concentrations, temperature, and pH, generally only the initial conditions can be manipulated in batch culture. Inoculum size and initial conditioned medium concentration represent two important considerations for optimal batch production. Two hybridoma cell lines were used to assess the impact of these initial conditions on population growth and monoclonal antibody productivity in suspension batch culture. Varying initial cell concentration over the range of 1.0 × 105 cells mL-1 to 3.0 × 105 cells mL-1 did not affect maximum product titre or maximum volumetric cell-hours attained. Initial percent of conditioned medium up to 40 percent strongly impacted on population growth and productivity, with initial levels of 30 to 40% conditioned medium reducing or eliminating lag phase and increasing average viable cell density. However, specific productivity and product titre declined with increasing initial percent conditioned medium, even on a per volume of fresh medium basis. Glutamine and glucose depletion or ammonia toxicity could cause depressed product titres when conditioned medium is used. Glutamine and glucose levels can easily be replenished in conditioned medium at minimal cost, and ammonia can be removed. Specific productivity was higher during cyclic batch operating mode than during batch operating mode. This may be because cyclic batch operating mode results in an incidental volume of conditioned medium at the beginning of each cycle. A two stage, cyclic-batch/batch operating mode can be employed to fully utilize medium and maximize product titre.
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  • 120
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    Journal of applied phycology 11 (1999), S. 293-299 
    ISSN: 1573-5176
    Keywords: organismic determinants ; Gracilaria gracilis ; Rhodophyta ; regeneration ; growth ; mariculture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The growth of Gracilaria gracilis (Stackhouse) Steentoft, Irvine et Farnham was examined by studying the effect of organismic determinants such as thallus length, position along the thallus and branching. Knowledge of these factors is essential in order to increase production from suspended seaweed rafts seeded with vegetative G. gracilis fragments. Seeding netlons with seaweed material freshly collected from subtidal populations provided up to 30% higher relative growth rates than seaweed maintained on the netlons for successive months. Initial seedstock length greatly affected growth rates and yields such that 30-cm thalli fragments resulted in growth rates 14% higher than for 10-cm fragments. This difference is suggested to be due to the higher contribution to overall biomass by growth of lateral branches. Comparisons of the growth of apical and basal fragments suggest that growth takes place over the entire length of the thallus, but that the apex contributes more to overall elongation than does the proximal part. The removal of apical meristems resulted in an enhanced branching frequency with production of four times as many branches as intact fragments. Evidence is also provided for extensive morphological differentiation following long periods of rapid growth. These thalli have very high frequency of branching, are hollow due to the disintegration of medullary cells and are considered to be completely senescent. These factors have implications for the successful cultivation of G. gracilis on commercial mariculture systems.
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  • 121
    ISSN: 1572-9788
    Keywords: lectins ; insect resistance ; transgenic plants ; potato (Solanum tuberosum) ; Lepidoptera ; Homoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of concanavalin A (ConA), a glucose/mannose-specific lectin from jackbean (Canavalia ensiformis), on insect crop pests from two different orders, Lepidoptera and Homoptera, were investigated. When fed to larvae of tomato moth (Lacanobia oleracea) at a range of concentrations (0.02–2.0% of total protein) in artificial diet, ConA decreased survival, with up to 90% mortality observed at the highest dose level, and retarded development, but had only a small effect on larval weight. When fed to peach-potato aphids (Myzus persicae) at a range of concentrations (1–9μM) in liquid artificial diet, ConA reduced aphid size by up to 30%, retarded development to maturity, and reduced fecundity (production of offspring) by 〉35%, but had little effect on survival. With both insects, there was a poor correlation between lectin dose and the quantitative effect. Constitutive expression of ConA in transgenic potatoes driven by the CaMV 35S promoter resulted in the protein accumulating to levels lower than predicted, possibly due to potato not being able to adequately reproduce the post-translational processing of this lectin which occurs in jackbean. However, the expressed lectin was functionally active as a haemagglutinin. Bioassay of L. oleracea larvae on ConA-expressing potato plants showed that the lectin retarded larval development, and decreased larval weights by 〉45%, but had no significant effect on survival. It also decreased consumption of plant tissue by the larvae. In agreement with the diet bioassay results, ConA-expressing potatoes decreased the fecundity of M. persicae by up to 45%. ConA thus has potential as a protective agent against insect pests in transgenic crops.
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  • 122
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    Journal of applied phycology 11 (1999), S. 551-558 
    ISSN: 1573-5176
    Keywords: Nodularia ; growth ; hepatotoxin production ; nodularin ; salinity ; irradiance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The objective of this work was to determine the influence of total dissolved solids/salinity (TDS mgL-1) on growth and biomass specific rates of nodularin (hepatotoxin) production by Nodularia spumigena 001E isolated from Lake Alexandrina, South Australia. Maximum biomass yield (dry matter, chlorophyll a and particulate organic carbon/POC) at 80 μmol photon m-2 s-1 was recorded at 3300 mg TDS L-1 and decreased at salinities above or below this value (p 〈 0.05). The maximum biomass yield (dry matter and chlorophyll a) at 30 μmol m-2 s-1 occurred at a higher salinity of 9900 mg TDS L-1. Cultures grown at 80 μmol m-2 s-1, at a TDS〉 6600 mg L-1, had significantly (p 〈 0.05) lower nodularin content (ml-1 medium) than cultures grown at the same salinities at 30 μmolm-2 s-1. The maximum total toxin concentration (mL-1 medium) occurred at 9900 and 3300 mg TDS L-1 at 30 μmol m-2 s-1and 80 μmol m-2 s-1 respectively. Toxin per unit biomass, expressed as dry matter, chlorophyll a and POC was similar for cultures grown at 30 μmol m-2 s-1 or 80 μmol m-2s-1 at salinities 〈 6600 mg TDS L-1. At salinities 〉 9900 mg TDS L-1 the toxin content per unit biomass decreased at both irradiances, however, cultures grown at 30 μmol m-2s-1 had a higher toxin content than those grown at 80 μmol m-2 s-1. The results indicate that not only do changes in irradiance and salinity directly influence growth and toxin production but that changes in irradiance affected the influence of salinity.
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  • 123
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    Water resources management 13 (1999), S. 73-84 
    ISSN: 1573-1650
    Keywords: chlorophyll ; growth ; rice crop ; spectral reflectance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Notes: Abstract Relations among spectral reflectance, chlorophyll ‘a’, and growth of rice plants grown on irrigated light textured soil in a semi arid region are presented here. There was a linear relation between spectral reflectance and rice plant height (r = 0.97), for band 1 (0.45–0.52 μm) reflectance values. On the other hand, in bands 2 (0.52–0.60 μm) and 3 (0.63–0.69 μm), reflectance values decreased until 70 days after planting (DAP) and then increased during the reproductive phase of the crop. The near infrared band 4 (0.76–0.90 μm) showed a maximum reflectance at 59 DAP (panicle initiation stage) and a decline in reflectance thereafter through maturity. The peak value of IR/R ratio was 16.39 at 62 DAP during the early reproductive phase; thereafter, it declines gradually with the maturity of the crop. Chlorophyll ‘a’ concentration was high during early growth (vegetative and early reproductive stages) and decreased during the flowering and maturity stages. The rice plant canopy show a high chlorophyll ‘a’ concentration at 64 and 59 DAP for sites A and B, respectively. Chlorophyll ‘a’ concentration is higher in site A plant canopies than it is in site B during the entire crop cycle. A good inverse correlation (r = 0.91) has been found between chlorophyll ‘a’ and band 1, while the IR/R ratio and the normalised difference vegetation index (NDVI) showed a relationship (r = 0.78) with the chlorophyll ‘a’ concentration during the crop cycle. Band 2, 3 and 4 radiance values show a biphasic linear relationship with chlorophyll ‘a’ concentrations, negative for early growth and positive for flowering and maturity stages. Results indicate that the period between 66 to 70 DAP is most suitable for the assessment of rice crop yield, based on chlorophyll ‘a’ concentration.
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  • 124
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    Aquaculture international 7 (1999), S. 251-260 
    ISSN: 1573-143X
    Keywords: bacterial biofilm ; common carp (Cyprinus carpio) ; growth ; rohu (Labeo rohita) ; Tilapia (Oreochromis mossambicus)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Experiments were conducted to enhance the growth of common carp (Cyprinus carpio), rohu (Labeo rohita) and Mozambique tilapia (Oreochromis mossambicus) through use of sugarcane bagasse as substrate. Bagasse was suspended in water with or without supplementation with fertilizers. Bagasse supplemented with cattle dung and urea favoured higher zooplankton production and significantly (p 〈 0.05) increased fish growth by over 50% compared to bagasse or fertilizers on their own. This higher production of fish is attributed to bacterial biofilm promoted on the substrate which, apart from forming food for zooplankton and fish, contributed to improved water quality by lowering ammonia.
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  • 125
    ISSN: 1573-143X
    Keywords: Convict cichlid (Cichlasoma nigrofasciatum ; environmental UV effects ; growth ; ultraviolet radiation ; UVA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The long term (two generations) effect an enhanced (5%) sub-lethal ultraviolet-A (320–400 nm) irradiation on growth and survival rate of a convict cichlid (Cichlasoma nigrofasciatum is presented. Two UVA irradiated populations (G1-UVA and G2-UVA) and two control populations (G1-control and G2-control) were observed from age 60 to 403 days. The UVA populations were exposed to enhanced UVA irradiation from 4 months of age (G1-UVA and from egg's state (G2-UVA). The specific growth rates (daily weight and length increase) and the condition factors of the populations were not affected by the enhanced UVA, it depended more on the population size. At the adapted temperature range (27–29 °C) the survival rate of those populations was not affected by enhanced UVA. A combined effect of enhanced UVA and increased temperature (31–32 °C) was responsible for a high mortality rate in the GI-UVA. A total of 67% of the G1-UVA population died just within a week of exposure to high temperature.
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  • 126
    ISSN: 1573-143X
    Keywords: body composition ; dietary protein ; fecundity ; gonad maturation ; growth ; Nile tilapia (Oreochromis niloticus)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Six-week-old Nile tilapia (Oreochromis niloticus) fry with an average weight (SD) of 0.51(0.2) g were reared for 140 days on five formulated, isocaloric diets of different protein levels (25, 30, 40 and 45% by dry weight). Fish fed diets of higher protein levels (40 and 45%) showed better growth and feed conversion ratio than those on lower protein levels. Fast-growing fish matured earlier. Maturation rate was affected by the dietary protein levels. Males matured earlier than females: the first mature males were recorded when they were 14 weeks old, whereas the females matured after 18 weeks. In both sexes, mean percentage of mature fish rose with increasing dietary protein level, the percentage of mature males being higher than that of the females. Similarly, the percentage of mature fish rose with the increasing age of fish, with more than 50% males and females mature at the age of 22 and 24 weeks, respectively, the exception being the 25% protein diet fed fish, where the percentage of mature fish was below 50%. In all treatments, spawning was initiated when the fish were 22 weeks old. Smallest size at spawning of males and females was 9.2 cm (13.1 g) and 8.1 cm (8.9 g), respectively, and dietary protein levels influenced the size of fish at first maturity. For both sexes, no difference was found in the gonado-somatic index (GSI) among different treatments. Fecundity increased with increasing dietary protein levels, but significant differences were found only between 40–45% and 25–35% dietary protein levels. The relative fecundity (eggs g−1 female) was higher at the lower dietary protein levels (25–35%) than at the higher dietary protein levels (40–45%). The dietary protein levels did not have any significant influence on the size and weight of mature eggs. The chemical composition of fish and mature ovaries was significantly influenced by the dietary protein level.
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  • 127
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    Aquaculture international 7 (1999), S. 179-187 
    ISSN: 1573-143X
    Keywords: feeding ; growth ; larvae ; micro-algae ; pearl oyster (Pinctada margaritifera L.) ; survival
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This paper reports on an experiment to determine growth and survival of blacklip pearl oyster, Pinctada margaritifera (L.), larvae fed a 1:1 mixture of Isochrysis aff. galbana clone T-ISO and Pavlova salina at six different densities (1, 2, 5, 10, 20 and 30 × 103 cells ml-1. Larval growth and survival were assessed every four days over a 20–day period. Exponential and logistic regression models were fitted to the growth and survival responses, respectively. Overall growth of larvae fed ≥5 × 103 cells ml-1 was significantly greater (p 〉 0.01) than growth of larvae reared at other algal densities. The optimal food ration for maximum larval growth was 20 × 103 cells ml-1, which resulted in larvae with antero-posterior shell length of 230 μm after 20 days. These larvae were significantly larger (p 〉 0.05) than those in all other treatments at the end of the experiment. Survival of larvae fed 0, 1 and 2 × 103 cells ml-1 was significantly lower than that of larvae in all other treatments at the end of 15 days (p 〉 0.01). Maximal survival (8%) over the 20 day period was shown by larvae fed 10 × 103 cells ml-1, while lower survival was shown by larvae fed 2 × 103 cells ml-1 (2%) and 1 × 103 cells ml-1 (0%).
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  • 128
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    Aquaculture international 7 (1999), S. 201-205 
    ISSN: 1573-143X
    Keywords: fingerlings ; growth ; light regimes ; photoperiod ; silver catfish (Rhamdia quelen) ; survival
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 129
    ISSN: 1573-143X
    Keywords: gilthead sea bream (Sparus aurata) ; growth ; hybridization ; red porgy (Pagrus pagrus) ; survival
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Hybridization between gilthead sea bream, Sparus aurata, and red porgy, Pagrus pagrus, was undertaken and viable hybrids were produced by an artificial hormonal-induced cross. The hybrids, from the cross of female red porgy and male gilthead sea bream (Pp × Sa), survived through the yolk-sac larval stage. The hybrids produced from the cross of female gilthead sea bream and male red porgy (Sa × Pp) presented higher mortality in the larval stage (98.4%) compared to gilthead sea bream (80.0%) and red porgy (92.5%). Afterwards, the Sa × Pp hybrid was compared to the offspring of its parental species under the same rearing conditions (tanks of 500 l capacity, natural light, ambient temperature, self-feeding with commercial feed) in a 40–week experiment. In this comparative study, the Sa × Pp hybrid had intermediate mortality rate (6%), compared to gilthead sea bream (0%) and red porgy (21%). The growth profile of this hybrid resembled the slow growing phases of its parents (red porgy in warm period and gilthead sea bream in cold period). The feed conversion ratio between fish forms was not significantly different (1.54–1.84 in the Sa × Pp hybrid, 1.53–1.56 in the maternal species and 1.33–1.40 in the paternal one). The body weight variation in the Sa × Pp hybrid was quite high (31.7–37.9%), compared to that in gilthead sea bream (19.7–24.7%) and red porgy (18.0–31.3%). With respect to body coloration, the Sa × Pp hybrid was distinguishable and classified into groups of red porgy-like (55.1%), gilthead sea bream-like (40.6%), and intermediate forms (4.3%). The preliminary results of this study proved that the produced Sa × Pp hybrid had too low a performance for aquaculture use, but the interesting consequent hereditary potential could be a useful tool in fish hybridization practice.
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  • 130
    ISSN: 1573-5036
    Keywords: 2 ; 4-D ; germination ; growth ; salinity-tolerance ; seed treatment ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Experiments were conducted under laboratory and greenhouse conditions to study the effect of 2,4-D on rooting and salinity tolerance of wheat. Seeds of one commercial wheat (Inqalab-91) and three salt-tolerant wheat lines (WL-41, WL-359, and WL-1073 developed through wide hybridization) were included in the study. Preliminary and short-term experiments were conducted to determine the level of 2,4-D (administered through seed soaking for 24.5 h. at 25 °C in the dark) at which the maximum number of roots emerged. Under hydroponic conditions, 2,4-D treatment of seeds caused an increase of 60 to 100% in the number of primary roots. The maximum increase in the number of roots was observed in one of the salt tolerant wheat lines (WL-41). The roots appeared in bunches but showed stunted growth at higher levels of 2,4-D. Dry matter accumulation decreased markedly; the effect was more pronounced in Inqalab-91 which is less tolerant to stress than other wheat lines. In all wheat types, allocation of dry matter to roots relative to shoot increased due to 2,4-D treatment. In soil, seeds treated with different levels of 2,4-D showed a germination delay of 1–3 days. Although the number of primary roots increased, 2,4-D treatment caused a decrease in total dry matter accumulation by plants grown for 40 days. In another experiment, conducted under greenhouse conditions, seed germination and growth of seedlings was significantly retarded in saline compared to that in non-saline (normal) soil. Initially, the pace of germination of treated seeds as well as seedling growth was slower in both soils, but after six weeks, the leaf area of seedlings raised from treated seeds was greater than those raised from untreated seeds. Towards maturity, plants arising from treated seeds developed wider and longer flag leaves leading to enhanced yield. Root biomass decreased in saline soil as compared to normal soil. However, 2,4-D treatment caused a substantial increase in root biomass in saline soil and the roots were harder in texture in wheats other than Inqalab-91. Seed treatment with 2,4-D led to a significant improvement in the number of productive tillers, yield of straw and grain, and grain protein content of all wheats grown in saline soil. Plants grown in normal soil did not show any marked effect of seed treatment on grain yield and other agronomic parameters. The four wheats showed substantial differences for different parameters but the salt tolerant wheat lines performed better compared to the commercial variety Inqalab-91.
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    Plant and soil 217 (1999), S. 1-15 
    ISSN: 1573-5036
    Keywords: anchorage ; development ; growth ; root systems ; symmetry ; tree stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The stability of shallowly rooted trees can be strongly influenced by the symmetry of the ‘structural’ system of woody roots. Root systems of forest trees are often markedly asymmetric, and many of the factors affecting symmetry, including root initiation and the growth of primary and woody roots, are poorly understood. The internal and environmental factors that control the development, with respect to symmetry and rigidity, of shallow structural root systems are reviewed and discussed with particular reference to Sitka spruce (Picea sitchensis Bong. Carr.). Areas where there is insufficient knowledge are highlighted. A scheme is proposed that represents the root system as a set of spokes that are variable in number, size and radial distribution. Rigidity can vary between and along each of the spokes. The root system is presented as a zone of competition for assimilates, where allocation to individual roots depends upon their position and local variations in conditions. Factors considered include the production of root primordia of different sizes, effects of soil conditions such as the supply of mineral nutrients and water on growth of primary and woody roots, and the effect of forces caused by wind action on growth of the cambium, giving rise to roots which, in cross section, resemble I- or T-beams, and efficiently resist bending.
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  • 132
    ISSN: 1573-5028
    Keywords: aquaporin ; gene expression ; growth ; Oryza sativa ; plasma membrane intrinsic protein (PIP) ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Membrane intrinsic proteins facilitate movement of small molecules often times functioning as water channels. We have identified two genes from rice which encode proteins with characteristic features of plasma membrane intrinsic proteins (PIP). They possess six membrane-spanning domains, an NPA repeat, overall high sequence homologies and characteristic C- and N-terminal hallmark motifs which allowed assignment of OsPIP1a to the PIP1 subfamily and of OsPIP2a to the PIP2 subfamily. OsPIP1a and OsPIP2a showed similar but not identical expression patterns. The two genes were expressed at higher levels in seedlings than in adult plants and expression in the primary root was regulated by light. In internodes of deepwater rice plants which were induced to grow rapidly by submergence, transcript levels were slightly induced in the intercalary meristem (IM) and slightly reduced in the elongation zone (EZ) after 18 h. In internodes of GA-induced excised stem sections transcript levels transiently declined in the IM and EZ after 1 h and subsequently recovered to elevated levels after 18 h. GA also induced OsPIP expression in non-growing tissue after 18 h. In the IM of submergence-induced stem sections transcript levels remained constitutive. The different growth-promoting treatments showed no direct correlation between growth rate and OsPIP gene expression in dividing or expanding cells. In fact, treatment of excised stem sections with ABA or drought stress induced similar changes in OsPIP expression in the growing zone during the first 6 h as GA did. We conclude that regulation of OsPIP1a and OsPIP2a expression is not primarily controlled by growth. GA-induced growth may however change the water status of cells which in turn results in altered PIP abundance.
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  • 133
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    Plant and soil 216 (1999), S. 93-101 
    ISSN: 1573-5036
    Keywords: Acacia cyanophylla ; endomycorrhizae ; growth ; nutrition ; Rhizobia ; salinity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The behaviour of Acacia cyanophylla Lind. plants submitted to salinity stress was followed in the greenhouse. The plants were associated with indigenous symbiotic microorganisms isolated from the coastal dunes of the Souss-Massa region. A two months period of salinity had a large negative impact on plant growth and acquisition of macro nutrients. However, the study underlined the role of the microbial inoculum for the plant in the achievement of salt tolerance. An isolate of Bradyrhizobium sp., RCM6 (R1), originating from the Massa dunes, was highly efficient in improving growth and nutrition of the A. cyanophylla. Double inoculation with the rhizobia and an endomycorrhizal complex, isolated from the Lamzar dunes had a clear additional positive effect, i.e. the fungi further increased the tolerance of the A. cyanophylla plants to salinity.
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    New forests 18 (1999), S. 301-314 
    ISSN: 1573-5095
    Keywords: chilling stress ; drought stress recovery ; field performance ; growth ; morphology ; nursery culture ; tree nursery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Five week old white spruce [Picea glauca (Moench) Voss] seedlings were placed within controlled-temperature growth chambers, and subjected to one of four periodic, chilling treatments for approximately 16 weeks. The treatments differed in the frequency of 24 hour exposure to 5 °C the seedlings received, and ranged from no chilling (control), to once every two, three or four days. After 25 weeks of growth, the control seedlings were significantly taller than all chilling-exposed seedlings, but stem diameters were similar. Chilling did not affect either needle length or needle density compared to control seedlings. During a water deficit test, frequently chilling-exposed seedlings maintained significantly higher mid-day shoot water potentials under extreme water stress. Re-watering after the drought resulted in all chilling-treated seedlings recovering faster to pre-stress shoot water potential levels than the control seedlings. Measurements of new root growth after 21 days at 10 °C indicated that previous chilling exposure delayed or inhibited new root production.
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  • 135
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    Plant growth regulation 29 (1999), S. 101-112 
    ISSN: 1573-5087
    Keywords: ABA ; cell wall-associated peroxidase ; expansins ; growth ; pH ; XET
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Over the past decade it has become clear that we cannot always explain the observed reduction in leaf expansion rates during drought by measuring the plant's water relations. This has led us to question the possibility of a role for the cell wall and its biochemical machinery in controlling the rate of leaf expansion during drought. However, if we are to reject or modify previous assumptions regarding the control of leaf expansion during drought, then we must offer alternative explanations. This article addresses recent work from this laboratory and in the literature, concerning the involvement of cell wall-enzymes, pH and abscisic acid (ABA) in regulating leaf expansion during water deficit.
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    Hydrobiologia 392 (1999), S. 205-215 
    ISSN: 1573-5117
    Keywords: Dreissena ; hypoxia ; thermal stratification ; survival ; growth ; distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Experiments and field surveys were conducted in Hargus Lake (Ohio, U.S.) to investigate the effect of lake stratification on the survival, growth and distribution of zebra mussels. During the lake stratification period, relatively stable temperature and dissolved oxygen (DO) gradients persisted across the water column, allowing us to examine the chronic effect of hypoxia on zebra mussels. Zebra mussels were incubated in cages and suspended at different depths in the water column at both pelagic (max. depth = 12 m) and littoral (max. depth = 3.5 m) sites from April 18 to September 28, 1994. No mussel survived to the end of the experiment in cages ≥ 5.5 m, whereas the highest survival rate (76%) occurred at 5 m depth where temperature and DO remained fairly stable for at least 3 months. The threshold oxygen level for survival was between 1.0–1.7 mg l-1 when water temperature was at about 17–18 °C. While zebra mussels′ survival rate was not affected under the sublethal hypoxic conditions, their growth was greatly retarded by poor water quality. The field survey showed that the zebra mussels and macrophytes had about the same distribution and their biomasses were positively related. The percentage of mussels in aggregates increased towards their maximum distribution depth. The maximum distribution depth of the naturally occurring zebra mussels was only 2.8 m, whereas the adult mussels could survive the entire stratification period when being artificially placed on the 3.5 m bottom, and young mussels could colonize the 3.5 m bottom if solid substrates were provided. We conclude that lack of substrate, rather than hypoxia, was the limiting factor of zebra mussel distribution above 5 m depth in Hargus Lake.
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    Hydrobiologia 401 (1999), S. 265-277 
    ISSN: 1573-5117
    Keywords: nucleic acids ; fish larvae ; RNA/DNA ; condition ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nucleic acid analysis has provided useful tools to study recent growth and mortality of young fishes and their responses to environmental variability. The ratio of RNA–DNA (R/D) has been shown to respond to changes in feeding conditions and growth after periods as short as 1–3 days in a variety of fish species. The earliest studies used primarily UV-based methods, but most investigators now use more sensitive, fluorometric dye-binding assays to estimate RNA and DNA in individual larvae. These newer methods are very sensitive to procedural details and choice of standards. Analytical methods, normalization and calibration procedures to optimize information obtained from nucleic acid analysis are discussed. We present examples illustrating the technique's utility, and problems encountered when applying nucleic acid-based indices to fish larvae and early juveniles. The wide use of R/D analysis in studies of fish early life stages, together with a proliferation of analytical methods, demands a major intercalibration exercise.
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  • 138
    ISSN: 1573-5117
    Keywords: zebra mussel ; intermittent halogenation ; Dreissena polymorpha ; growth ; settlement ; survival
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of intermittent (2–4 h/day) treatments with chlorine or bromine at levels of 0.5 and 1.0 mg/L (total residual oxidant) upon settling and growth of veliger larvae of the zebra mussel, Dreissena polymorpha, was examined in two successive flow-through studies. The work was conducted in a field laboratory which received a constant supply of water from western Lake Erie. Veliger densities in the water at the field site peaked at 530/L, while mussel densities on settling monitors reached 147,100/m2 over the course of the two studies (early July to late September 1991). In Study 1, a 2-h daily treatment with 1.0 mg/L chlorine reduced mussel settling by 91% as compared with controls, although mussel densities of up to 6,000/m2 still occurred. Treatment with 0.5 mg/L chlorine for 4 h/day produced a similar reduction in mussel settling during the second study. Bromine was less effective than chlorine at reducing settling of veligers. Mussels which remained settled in the treatment tanks had growth rates similar to controls, reaching 2–4 mm in length over a 30-day period. The intermittent halogen treatments similarly had no effect on whole body glycogen levels or growth of adult D. polymorpha. The intermittent chlorination regime used may therefore delay, but would not prevent a mussel fouling problem.
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  • 139
    ISSN: 1573-5117
    Keywords: Gracilaria multipartita ; seasonal variation ; growth ; chemical composition ; agar
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The biology and agar composition and properties of Gracilaria multipartita, a common species along the coasts of Morocco, have been studied on samples collected monthly for one year. Growth of the alga was maximum in spring and autumn, and the seaweed partially decayed after its maximum fertility was reached in June and October. The agar content and composition showed seasonal variations. The agar content was maximal in winter (30% dw), and decreased during the growth periods to minima in June and October (25% dw) which also corresponded to the maxima of fertility. The agar composition was characterized by high 6-O-methyl galactose (38–59 mol%) and 3,6 anhydrogalactose (24–39%) contents together with galactose (12.6–25.7 mol%) and sulphate (24–5.0% dw). The gel strength varied between 246 and 511 g cm−2 and increased after alkali treatment to reach a maximum of 880 g cm−2. The gel stre ngth decreased after the alga reached its maxima of fertility, indicating a possible relationship between growth, fertility and agar metabolism. The content and quality of agar from G. multipartita growing in Morocco are suitable for an industrial use of the seaweed for the production of food-grade agar.
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  • 140
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    Environmental biology of fishes 54 (1999), S. 325-336 
    ISSN: 1573-5133
    Keywords: population structure ; reproduction ; growth ; mortality ; fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Black seabream, Spondyliosoma cantharus, caught off the Canary Islands is characterized by a protogynous hermaphroditism. The size range of the catches is between 8 and 40 cm, with a main distribution between 16 and 24 cm. The mean length of the individuals shows an increase with increasing depth. Males:females ratio is unbalanced in favour of females (1:2.18). The reproductive season extends from late autumn to mid spring, with a peak in spawning activity in January–February. A dichromatism is found between males and females during the spawning season. Males reach maturity at a larger total length, 22.7 cm (3 years old), than females, 17.3 cm (2 years old). Morphometric relationship between length and mass for the whole population is described by the parameters: a=0.00732, and b=3.24747. Otoliths age readings indicate that the exploited population consists of eleven age groups (0–X years), including a very high proportion of individuals between 2 and 3 years old. The von Bertalanffy growth parameters for the whole population are: L∞=43.35 cm, k=0.24 year−1, and t0=−0.11 year. The rates of total, natural and fishing mortality are 1.36, 0.52 and 0.84 year−1, respectively. The length at first capture is 16.8 cm. The exploitation rate indicates that the stock is overfished.
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  • 141
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    Environmental biology of fishes 55 (1999), S. 215-225 
    ISSN: 1573-5133
    Keywords: gonadosomatic index ; growth ; maturity ; migration ; mortality ; sex ratio ; spawning ; von Bertalanffy growth model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Aspects of the life history of Labeo cylindricus in Lake Chicamba, a man made hydroelectric dam in central Mozambique were investigated. L. cylindricus was found to be a potadrometic, synchronous spawner concentrating in river mouths prior to migrating up flowing rivers in January to spawn. Length-at-(50%)-maturity was attained at a fork length (FL) of 96 mm for males and 98 mm for females, both within their first year of life. The adult sex ratio was found to be female dominated at 1 male:1.63 females. Scales were used to age the species, with two growth checks deposited annually. Growth was rapid and was best described by the von Bertalanffy growth model as L t =224.2(1−e-0.66(r+0.03)) mm FL. A maximum age of 4 years was observed for L. cylindricus with the largest male and female measuring 210 mm FL and 235 mm FL, respectively. The rate of natural mortality was estimated to be extremely high at 1.93 year-1 using length-converted catch curve analysis and is possibly a response to heavy predation by the introduced piscivore Micropterus salmoides.
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  • 142
    ISSN: 1573-5133
    Keywords: reproduction ; spawning ; age ; growth ; vermilion snapper ; Trinidad ; Tobago
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Reproduction and growth of the vermilion snapper, Rhomboplites aurorubens, were studied in Trinidad and Tobago. The smallest individual caught measured 145 mm total length (TL) and all fish appeared to be mature. It was not possible to precisely determine size at first maturity due to the use of macroscopic techniques. The smallest spent male and female measured 181 and 211 mm TL respectively, suggesting a size at first maturity below these sizes. Spawning occurred throughout the year, with a period of peak spawning from about June to November in the rainy season when river runoff increased. Sagittal otolith sections were used for age determination and the opaque ring, which was counted as the annulus, was deposited from January to May in the dry season. A total of 11 age groups between the ages of 2–12 years (155–505 mm total length) were found. The von Bertalanffy growth parameters were: L∞=532 mm, K=0.13 y−1, and t0=−0.17, where L∞ is the asymptotic length, K is the growth coefficient and t0 is the theoretical age at zero length. The relationship between weight (WT) and length (TL) was WT=3.43×10−5 TL2.82. Vermilion snapper in this study area appears to grow slower and attain a smaller asymptotic length, but has a longer lifespan than found in populations in higher latitudes. This may be attributed to different levels of exploitation, which may be higher in the latter areas.
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  • 143
    ISSN: 1573-5168
    Keywords: growth ; mass spectrometry ; 15N-phenylalanine ; protein synthesis ; stable isotope technology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In vivo rates of protein synthesis in fish have predominantly been measured using a single flooding dose injection of a solution containing 3H-Phenylalanine as a tracer. However, use of a radiolabelled tracer restricts the application of this technique to controlled laboratory conditions. In this study, the flooding dose technique was used to successfully measure in vivo rates of protein synthesis in Atlantic salmon (Salmo salar L.) smolts utilising a stable isotope labelled amino acid, L-[15N]-Phenylalanine, as an alternative tracer. Methodologies are presented allowing the 15N enrichment in the free amino acid pool and in body protein to be measured using mass spectrometry. In this study, the fractional rates of protein consumption (kr), synthesis (ks) and growth (kg) of Atlantic salmon smolts (37 ± 1.4 g) were measured as 4.7, 3.3 and 1.6% day−1 respectively. Protein synthesis retention efficiency (kgks) and protein growth efficiency (kgkr)were calculated as 48.5 and 34.0% respectively. The ks, kgks and kgkr values obtained in this study were found to be within the range of values available in the literature for juvenile salmonid fish of a comparable size. The use of L-[15N]-Phenylalanine provides an alternative tracer to radiolabelled amino acids for measuring in vivo rates of protein synthesis in fish using the flooding dose' technique and also allows studies of fish protein metabolism to be carried out in environmentally sensitive (field and laboratory) conditions where the use of radiolabels is prohibited.
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  • 144
    ISSN: 1573-5168
    Keywords: Atlantic salmon ; growth ; individual feed consumption rate ; plasma insulin ; protein synthesis capacity ; trypsin isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Growth was found to be associated with the changes of trypsin activity in the pyloric caecal tissues and the level of plasma insulin in Atlantic salmon (Salmo salar L.). A decrease in trypsin activity accompanied by an increase in plasma insulin was detected one month before an enhanced growth was observed. There were significant relationships between weight specific consumption rate, plasma insulin levels and fish growth. The correlation of weight specific consumption rate was higher with growth rate (R2=0.7, p〈0.0001) than with plasma insulin concentration (R2=0.4, p〈0.0001). When the comparison was made between Atlantic salmon carrying and lacking the trypsin variant TRP-2*92, the fish with the variant had lower maintenance ration (p〈0.05), higher capacity for protein synthesis in the white muscle (p〈0.02), and a greater ability to utilize the feed at a restricted ration than the fish without the variant. In Atlantic salmon lacking the variant, both plasma insulin concentrations and growth rates were significantly lower (p〈0.05) in the fish fed 0.5% bw day−1 than those fed 1% bw day−1. Whilst the growth rates of TRP-2*92 salmon fed the different rations became similar one month after similar levels of plasma insulin were observed between them. The TRP-2*92 salmon may be defined as a high protein growth efficiency fish with low protein turnover rate. Genetic variation in trypsin isozyme pattern affects feed utilization, plasma insulin levels and growth in Atlantic salmon.
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  • 145
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    Journal of economic growth 4 (1999), S. 429-445 
    ISSN: 1573-7020
    Keywords: growth ; R&D ; education ; regime shift
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract The role of learning and R&D in economic development is addressed in an endogenous growth model. When human capital is below a threshold level, the model predicts that skills are accumulated as the only growth-generating activity, whereas both innovation activities and learning drive growth above this level. Hence, an endogenous regime shift is triggered when the level of human capital reaches the threshold level because it becomes profitable to innovate.
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  • 146
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    Journal of economic growth 4 (1999), S. 55-80 
    ISSN: 1573-7020
    Keywords: mercantilism ; growth ; taxation ; openness ; familiarity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract Nations close themselves voluntarily to varying degrees. Restrictions on the flow of ideas are difficult to understand, since open countries have higher relative incomes. This article provides an explanation based on the existence of two channels of public finance—traditional and mercantilistic. The latter refers to monopoly creation to provide a stream of government revenue. Strong, profitable monopolies require that the nation be closed to new ideas about technology and organization. The government sets the degree of restriction to balance current mercantilistic revenue with future revenue from traditional sources. The model is supported with numerical simulations and historical illustrations.
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  • 147
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    Journal of economic growth 4 (1999), S. 119-137 
    ISSN: 1573-7020
    Keywords: growth ; technology ; Solow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract Growth accounting breaks down economic growth into components associated with changes in factor inputs and the Solow residual, which reflects technological progress and other elements. After a presentation of the standard model, the analysis considers dual approaches to growth accounting (which considers changes in factor prices rather than quantities), spillover effects and increasing returns, taxes, and multiple types of factor inputs. Later sections place the growth-accounting exercise within the context of two recent strands of endogenous growth theory—varieties-of-products models and quality-ladders models. Within these settings, the Solow residual can be interpreted in terms of measures of the endogenously changing level of technology.
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  • 148
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    Journal of economic growth 4 (1999), S. 305-330 
    ISSN: 1573-7020
    Keywords: growth ; convergence clubs ; poverty trap ; cultural factors ; location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract This study investigates the sources of heterogeneity across a worldwide set of countries. Unspecified ex ante and unanticipated cultural (Protestant versus Catholic), geographical (continents), and institutional (OECD versus non-OECD) clubs emerge endogenously and naturally as homogeneous classes on the basis of their economic structure. The dynamics both within and across the identified groups of countries are consistent with multiple equilibrium-growth models proposed by, for instance, Azariadis and Drazen (1990), therefore strengthening the viability of the convergence club hypothesis. In particular, higher stages of development are, on average, non linearly associated with higher stages of growth.
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  • 149
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    Journal of economic growth 4 (1999), S. 81-111 
    ISSN: 1573-7020
    Keywords: growth ; investment ; regimes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract The absence of continuous regime type measures that focus on institutions rather than outcomes besets studies on whether democratic or authoritarian regimes grow faster. Additional shortcomings include the failure to consider development stages and the erroneous endogenous specification of regimes. Given panel data on 105 countries from 1960 to 1989, the effective party/constitutional framework measure does not correlate with growth or investment in the total sample. But considering development levels, some evidence indicates that discretion decreases growth in advanced areas, and, contrary to theory, inhibits investment in poorer countries. Also, single-party dictatorships have higher investment ratios but do not grow faster than party-less regimes.
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  • 150
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    Journal of economic growth 4 (1999), S. 213-232 
    ISSN: 1573-7020
    Keywords: education ; work experience ; self-employment ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract We examine the implications for growth and development of the existence of two types of human capital: entrepreneurial and professional. Entrepreneurs accumulate human capital through a work-experience intensive process, whereas professionals’ human capital accumulation is education-intensive. Moreover, the return to entrepreneurship is uncertain. We show how skill-biased technological progress leads to changes in the composition of aggregate human capital; as technology improves, individuals devote less time to the accumulation of human capital through work experience and more to the accumulation of human capital through professional training. Thus, our model explains why entrepreneurs play a relatively more important role in intermediate-income countries and professionals are relatively more abundant in richer economies. It also shows that those countries that initially have too little of either entrepreneurial or professional human capital may end up in a development trap.
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  • 151
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    Journal of economic growth 4 (1999), S. 331-349 
    ISSN: 1573-7020
    Keywords: growth ; fertility ; income distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract This article analyzes the interaction between growth and fertility via income distribution in a model in which fertility decisions are motivated by old-age support. It provides an explanation of the demographic transition of an economy from a stage of increasing fertility and low growth to a stage of low fertility, high human capital investments, and high growth.
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  • 152
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    Review of industrial organization 14 (1999), S. 391-396 
    ISSN: 1573-7160
    Keywords: Fate ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract Fate bringeth economic growth and malfeasance giveth its gains
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  • 153
    ISSN: 1573-143X
    Keywords: apparent digestibility coefficient ; fish meal ; growth ; Nile tilapa (Oreochromis niloticus) ; plant proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Apparent digestibility coefficient (ADC) values for a number of ingredients of plant or animal origin were obtained in order to formulate diets based on such values and to evaluate growth performance of Nile tilapia Oreochromis niloticus fed four experimental diets in which fish meal was gradually replaced by a mixture of other ingredients. The digestibility of various diet components was measured by using an inert marker in the feed and by using the Guelph faeces collecting system. ADC values of the ingredients tested were generally high, especially for fish meal. It was found that extruded pea seed meal (92.6%), defatted soybean meal (94.4%), full-fat toasted soybean (90.0%) and micronized wheat (88.6%) were the best vegetable proteins tested. Lupin seed meal and faba bean meal had similar ADC values for protein and energy. Groups of tilapia, initial mean body weight (SD) 6.7 (0.1) g, were fed experimental diets with the same digestible protein (DP) and digestible energy (DE) containing graded levels of a mixture of vegetable ingredients as partial or total replacement of fish meal protein. A growth trial was conducted over 12 weeks as partial or total replacement of fish meal protein. A growth trial was conducted over 12 weeks at a water temperature of 25 °C. Significant differences were observed for weight gain among tilapia fed diets D0, D33, D66 and D100 (containing only animal protein, 33, 66, and 100% of plant protein, respectively). No significant differences were observed for voluntary intake among tilapia fed diets D0, D33 and D66. These values were significantly lower than those observed for tilapia fed plant protein based diet (D100) and suggest the possibility of partial replacement of fish meal by vegetable proteins without negative effects.
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  • 154
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    Aquaculture international 7 (1999), S. 357-360 
    ISSN: 1573-143X
    Keywords: feed intake ; growth ; silver trevally (Pseudocaranx dentex) ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 155
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    Journal of chemical ecology 25 (1999), S. 2305-2312 
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Lymantriidae ; Lymantria fumida ; Lymantria monacha ; nun moth ; sex pheromone ; periodicity ; calling behavior ; reproductive isolation ; disparlure ; (7R,8S)-cis-7,8-epoxy-2-methyloctadecane ; (7S,8R)-cis-7,8-epoxy-2-methyloctadecane ; 2-methyl-Z7-octadecene ; (7R,8S)-cis-7,8-epoxy-octadecane ; (+)-monachalure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Extracts of pheromone glands from female Lymantria fumida were analyzed by coupled gas chromatographic–electroantennographic detection (GC-EAD) and by coupled GC–mass spectrometry (MS). The two compounds that elicited responses from male L. fumida antennae were identified as cis-7,8-epoxy-2-methyloctadecane (disparlure) and 2-methyl-Z7-octadecene (2me-Z7–18Hy). Field experiments in northern Japan demonstrated that synthetic (7R,8S)-cis-7,8-epoxy-2-methyloctadecane [(+)-disparlure] and 2me-Z7–18Hy are synergistic sex pheromone components of L. fumida. (7S,8R)-cis-7,8-Epoxy-2-methyloctadecane [(−)-disparlure] had no behavioral effect on male L. fumida. Traps baited with (+)-disparlure and 2me-Z7–18Hy captured male L. fumida between 21:00 and 24:00 hr, whereas traps baited with (+)-monachalure [(7R,8S)-cis-7,8-epoxy-octadecane], (+)-disparlure and 2me-Z7–18Hy attracted males of the nun moth, L. monacha L., between 02:00 and 04:00 hr. Both temporal separation of pheromonal communication and specificity of pheromone blends seem to contribute to the reproductive isolation of sympatric and coseasonal L. fumida and L. monacha.
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  • 156
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    Journal of chemical ecology 25 (1999), S. 2547-2559 
    ISSN: 1573-1561
    Keywords: Yponomeuta cagnagellus ; ermine moth ; Lepidoptera ; speciation ; specialization ; plant surface compounds ; oviposition ; host discrimination ; Euonymus europaeus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Yponomeuta cagnagellus is a phytophagous moth species specialized on Euonymus europaeus. Host discrimination by the adult female is an important aspect of host specialization and is based mainly on the distinctive secondary chemistry of host and nonhosts. This paper describes a bioassay that was developed to study the effect of isolated plant surface compounds on Yponomeuta oviposition. Adult moths recognize their hosts through chemical stimuli on the leaf or twig surface. Relatively apolar compounds extracted from the host twig surface by washing in dichlormethane do not stimulate oviposition. More polar, methanol-soluble compounds do, and this stimulation is dose dependent. Moths are able to recognize hosts solely by their surface compounds: females show a strong preference for artificial twigs treated with methanolic extracts of their hosts compared to those treated with methanolic extracts of nonhosts Crataegus monogyna and Prunus spinosa (both of which are hosts for closely related Y. padellus). Shape and surface characteristics of the oviposition substrate also influence oviposition. The substrate needs to resemble the basic form of a twig (i.e., cylindrical), and females prefer a coarse surface with irregularities over a smooth one.
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  • 157
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    Journal of chemical ecology 25 (1999), S. 1233-1245 
    ISSN: 1573-1561
    Keywords: Swallowtail butterfly ; Papilionidae ; Papilio troilus ; Lepidoptera ; Lauraceae ; Sassafras albidum ; oviposition stimulant ; 3-trans-caffeoyl-muco-quinic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Female butterflies of the spicebush swallowtail, Papilio troilus, are specialists, ovipositing on plants in the family Lauraceae. Column chromatography and HPLC were used to isolate an oviposition stimulant from the leaves of one of its hosts, Sassafras albidum. The stimulant was identified as 3-trans-caffeoyl-muco-quinic acid on the basis of FAB-MS and 1H NMR spectra as compared to a compound previously isolated from another plant. It was not active alone, but it increased the oviposition activity of butterflies when combined with other stimulant(s) at a concentration of 7 ng/mm2 leaf surface area. Other caffeoylquinic acid isomers tested did not have this effect. This is the first report of a swallowtail contact oviposition stimulant from a plant in the family Lauraceae.
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  • 158
    ISSN: 1573-1561
    Keywords: European corn borer ; Ostrinia nubilalis (Hübner) ; growth ; development ; fecundity ; oviposition ; behavior ; eggs ; DIMBOA ; water extract ; plant extract ; maize ; Peruvian maize ; maize accessions ; host plant resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Twelve Peruvian maize, Zea mays, accessions were selected because of their relatively high level of field resistance to first-generation European corn borer (ECB), Ostrinia nubilalis, larval leaf-feeding. Water extracts of freeze-dried, powdered, leaf tissue were incorporated into a standard ECB diet, fed to larvae, and the effects on larval growth, development, and fecundity were measured. Larval and pupal weights were monitored as were the time elapsed in the larval, pupal, and adult stages. Adult fecundity and egg fertility were recorded. The experiment was a randomized block design (larvae and pupae) or a completely randomized design (adults) and analyzed with ANOVA (α = 0.05). Pairwise comparisons were made between groups of insects grown on diets containing extracts from the Peruvian lines, a standard diet, or diets containing extracts of a known susceptible inbred, and a known resistant inbred line. Survival was analyzed with a chi-squared test (α = 0.05). Two Peruvian accessions significantly reduced female larval and pupal weights, extended pupal and adult development time, and decreased survival of pupae and adults. Water extracts also had a pronounced impact on males; two accessions significantly reduced pupal weight and extended the time required to pupate, and one reduced male survival to adults. The results indicate that water-soluble factors from resistant Peruvian accessions inhibit the growth, developmental time, and survival of ECB. These resistance factors could be useful in the development of maize germplasm with insect-resistant traits.
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    Journal of chemical ecology 25 (1999), S. 1343-1351 
    ISSN: 1573-1561
    Keywords: Host-plant volatiles ; apple ; Malus domestica ; reproductive behavior ; codling moth ; Cydia pomonella ; Tortricidae ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Apple volatiles stimulated pheromone release, oviposition, and upwind orientation in female codling moths, Cydia pomonella. Green apples increased the percentage of virgin females calling, the duration of female calling, and advanced the onset of egg-laying in gravid females. In a tube olfactometer, both virgin and mated females were more active in the presence of apple volatiles than in clean air. They responded by walking while wing-fanning; mated females showed a stronger attraction response than unmated females.
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  • 160
    ISSN: 1573-1561
    Keywords: Tritrophic interactions ; Lima bean ; Phytoseiulus persimilis ; Tetranychus urticae ; Spodoptera exigua ; Acari ; Lepidoptera ; infochemicals ; herbivore-induced plant volatiles ; nonprey herbivores ; feces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The effect of volatiles related to feeding activity of nonprey caterpillars, Spodoptera exigua, on the olfactory response of the predatory mites Phytoseiulus persimilis was examined in a Y-tube olfactometer. At a low caterpillar density (20 caterpillars on 10 Lima bean leaves), the predators were significantly more attracted to volatiles from infested leaves on which the caterpillars and their products were present or from infested leaves from which the caterpillars and their products had been removed when compared to volatiles from uninfested leaves. The predators, however, significantly avoided odors from 20 caterpillars and their products (mainly feces) removed from bean leaves. In contrast, at a higher caterpillar density (100 caterpillars on 10 Lima bean leaves), the predators avoided volatiles from caterpillar-infested bean leaves. Volatiles from infested leaves from which the caterpillars and their products had been removed were not preferred over volatiles from uninfested leaves. Volatiles from feces collected from 100 caterpillars were strongly avoided by the predators, while the behavior of the predatory mites was not affected by volatiles from 100 caterpillars removed from a plant. The data show that carnivorous arthropods may avoid nonprofitable herbivores. This avoidance seems to result from an interference of volatiles from herbivore products with the attraction to herbivore-induced plant volatiles.
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  • 161
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    Journal of chemical ecology 25 (1999), S. 1945-1960 
    ISSN: 1573-1561
    Keywords: Insect–plant interaction ; midgut alkalinization ; phylogenetic distribution ; Mecopterida ; Diptera ; Lepidoptera ; Trichoptera ; polyphenolics ; alkylating agents
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The phylogenetic distribution of reported midgut pH values among larval Mecopterida supports a model in which the common ancestor of this group possessed an alkaline midgut, with subsequent loss of this trait in the lineage leading to the muscomorphan Diptera. The relationship between midgut pH and diet guild rank within the Lepidoptera and Diptera was tested by assigning numerical values to diet guilds (i.e., fruit, grasses, herbs, trees and shrubs, and organic detritus). Lepidopteran superfamilies were found to differ significantly in both midgut pH and in diet guild rank. Regression of mean superfamily midgut pH against mean superfamily diet guild rank yielded an R 2 of 0.79 (N = 10), whereas regression of species midgut pH against species diet guild rank yielded an R 2 of only 0.15 (N = 60). Species feeding on foliage of plant taxa high in tannins and on Solanaceae have midgut pH values above 9, and midgut pH in species feeding on these taxa is positively related to diet guild. In contrast, species feeding on the foliage of plant taxa containing terpenes, DIMBOA, glucosinolates, and pyrrolizidine alkaloids have midgut pH values near 8, and midgut pH of these species is either not related to diet guild (all species) or is negatively related to diet guild rank when the analysis is limited to the Noctuoidea. The data suggest that decreased midgut pH in species feeding on plants containing terpenes, DIMBOA, glucosinolates, and pyrrolizidine alkaloids may be an adaptive response that overrides selection for high pH in the presence of tannins and that midgut pH may be one factor contributing to the limitation of the host plant range of many species of lepidopteran herbivores.
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    Journal of chemical ecology 25 (1999), S. 2535-2545 
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Lymantriidae ; Lymantria xylina ; Lymantria dispar ; Lymantria monacha ; Lymantria fumida ; sex pheromone ; reproductive isolation ; (7R,8S)-cis-7,8-epoxy-2-methyleicosane ; (7S,8R)-cis-7,8-epoxy-2-methyleicosane ; 2-methyl-Z7-eicosene ; (7R,8S)-cis-7,8-epoxy-2-methylnonadecane ; (7S,8R)-cis-7,8-epoxy-2-methylnonadecane ; (7R,8S)-cis-7,8-epoxy-3-methylnonadecane ; (7S,8R)-cis-7,8-epoxy-3-methylnonadecane ; disparlure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract cis-7,8-Epoxy-2-methyleicosane is a sex pheromone component of the Casuarina moth, Lymantria xylina Swinhoe. The compound was extracted from pheromone glands of female moths and was identified by coupled gas chromatographic–electroantennographic detection (GC-EAD) and GC–mass spectrometry. In field experiments in Taiwan, traps baited with either or both of (7R,8S)-cis-7,8-epoxy-2-methyleicosane (〉99% ee) [termed here (+)-xylinalure] and (7S,8R)-cis-7,8-epoxy-2-methyleicosane (〉99% ee) [termed here (−)-xylinalure] captured male L. xylina. Addition of further candidate pheromone components to xylinalure did not enhance its attractiveness. Demonstration of whether or not female L. xylina produce both optical isomers of xylinalure, and determination of the ratio, will require pheromone extract analyses on a chiral, enantiomer-separating column (as yet unavailable) or derivatization of epoxides in accumulated gland extracts. Attraction of male L. xylina to either enantiomer of xylinalure contrasts with enantiospecific production of, and/or response to, epoxy pheromones in congeners. With no other nocturnal lymantriid moth known in Taiwan to utilize xylinalure for pheromonal communication, enantiospecific “fine tuning” of xylinalure, or evolution of a more complex pheromone blend, may not have been necessary for L. xylina to maintain specificity of sexual communication. Racemic xylinalure will be appropriate for pheromone-based detection surveys of L. xylina in North America.
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  • 163
    ISSN: 1573-1561
    Keywords: Coniesta ignefusalis ; Acigona ignefusalis ; Lepidoptera ; Pyralidae ; sex pheromone ; (Z)-7-dodecen-1-ol ; (Z)-5-decen-1-ol ; (Z)-7-dodecenal ; (Z)-7-dodecenyl acetate ; (Z)-9-tetradecen-1-ol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Five active compounds were detected during analyses of ovipositor washings and effluvia from virgin female Coniesta ignefusalis moths by gas chromatography (GC) linked to electroantennographic (EAG) recording from a male moth. These were identified as (Z)-7-dodecen-1-ol (Z7–12:OH), (Z)-5-decen-1-ol (Z5–10:OH), (Z)-7-dodecenal (Z7–12:Ald), (Z)-7-dodecenyl acetate (Z7–12:Ac), and (Z)-9-tetradecen-1-ol (Z9–14:OH) by comparison of their GC retention times, mass spectra, and EAG activities with those of synthetic standards. Laboratory tests of dispensers for these compounds showed that release rates from polyethylene vials increased to relatively uniform values after three to four days, but release from septa was very rapid and nonuniform and decreased to low levels after two to three days. Trapping tests in Niger showed that the major component, Z7–12:OH, and two of the minor components, Z5–10:OH and Z7–12:Ald, were essential for attraction of male C. ignefusalis moths. The most attractive blend contained these three components in a 100:5:3.3 ratio in a polyethylene vial, which emitted the components in similar proportions to those produced by the female C. ignefusalis moth. Water traps baited with this blend containing 1 mg of Z7–12:OH caught more male C. ignefusalis moths than traps baited with newly emerged female moths. Addition of up to 10% of the corresponding E isomers of the pheromone components had no effect on catches, but addition of the other two minor components detected, Z7–12:Ac and/or Z9–14:OH, to the attractive blend at naturally occurring levels caused significant reductions in trap catch.
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  • 164
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Tortricidae ; Lobesia botrana ; grapevine moth ; wind tunnel ; behavior ; flight track recording ; (E)-7,(Z)-9-dodecadienyl acetate ; (E)-7,(Z)-9-dodecadien-1-ol ; (Z)-9-dodecenyl acetate ; (E)-9-docecenyl acetate ; 11-dodecenyl acetate ; pheromone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The behavioral responses of Lobesia botrana males to calling females, pheromone gland extracts, and synthetic sex pheromones were recorded in a wind tunnel. Gland extracts and synthetic pheromones were released from a pheromone evaporator. The numbers of males reaching the source and their flight tracks in response to calling females and pheromone gland extracts were compared to those of synthetic blends. Upwind flights to natural sex pheromone were straighter and faster than to a three-component blend of (E)-7,(Z)-9-dodecadienyl acetate (E7,Z9–12:Ac), (E)-7,(Z)-9-dodecadien-1-ol (E7,Z9–12:OH), and (Z)-9-docecenyl acetate (Z9–12:Ac) (100:20:5). The optimum ratio of E7,Z9–12:OH and Z9–12:Ac to E7,Z9–12:Ac was found to be 5% and 1%, respectively. An additional seven compounds identified in the sex pheromone gland were investigated for their biological activity. Two unsaturated acetates, i.e., (E)-9-dodecenyl acetate (E9–12:Ac) and Δ11-dodecenyl acetate (Δ11–12:Ac), increased the number of males reaching the source as well as straightness, linear velocity, and decreased the track angle of upwind flight. Optimum response was obtained by releasing 10 pg/min E7,Z9–12:Ac in a mixture with 0.5 pg/min E7,Z9–12:OH, 0.1 pg/min Z9–12:Ac, 0.1 pg/min E9– 12:Ac and 1 pg/min Δ11-12–Ac. The saturated acetates previously identified in the female glands were biologically inactive.
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  • 165
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    Biodegradation 10 (1999), S. 177-191 
    ISSN: 1572-9729
    Keywords: AQUASIM ; biodegradation ; biofilm ; growth ; kinetics ; methane ; modelling ; nitrification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This article discusses the growth of methanotrophic biofilms. Several independent biofilm growths scenarios involving different inocula were examined. Biofilm growth, substrate removal and product formation were monitored throughout the experiments. Based on the oxygen consumption it was concluded that heterotrophs and nitrifiers co-existed with methanotrophs in the biofilm. Heterotrophic biomass grew on soluble polymers formed by the hydrolysis of dead biomass entrapped in the biofilm. Nitrifier populations developed because of the presence of ammonia in the mineral medium. Based on these experimental results, the computer program AQUASIM was used to develop a biological model involving methanotrophs, heterotrophs and nitrifiers. The modelling of six independent growth experiments showed that stoichiometric and kinetic parameters were within the same order of magnitude. Parameter estimation yielded an average maximum growth rate for methanotrophs, μm, of 1.5 ± 0.5 d−1, at 20 °C, a decay rate, bm, of 0.24 ± 0.1 d−1, a half saturation constant, $${\text{K}}_{{\text{S(CH}}_{\text{4}} {\text{)}}} $$ , of 0.06 ± 0.05 mg CH4/L, and a yield coefficient, $$Y_{CH_4 } $$ , of 0.57 ±: 0.04 g X/g CH4. In addition, a sensitivity analysis was performed on this model. It indicated that the most influential parameters were those related to the biofilm (i.e. density; solid-volume fraction; thickness). This suggests that in order to improve the model, further research regarding the biofilm structure and composition is needed.
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    GeoJournal 49 (1999), S. 279-288 
    ISSN: 1572-9893
    Keywords: globalisation ; growth ; information technology ; infrastructure ; knowledge ; modernisation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography
    Notes: Abstract This study examines the modernising role played by infrastructure and the transition of emphasis from physical infrastructure to knowledge-based infostructure as a source of economic growth in Singapore, noting the factors behind the change in strategy. Occupying a strategic sea route location and serving a resource-rich hinterland, Singapore inherited from the old British empire a well-established infrastructure when it achieved self-government in 1959. Since 1965, Singapore's development has been led by a pro-business developmentalist government, and strongly linked to technological advances in the West on which it also relies heavily for its exports and multinational corporation investments. From the 1980s, the government has launched a series of pro-IT plans to prepare the city-state's transition to an `intelligent island', and a regional hub for high-technology, and international financial transactions, a full-swing state-initiated strategy to leap from a semi-peripheral economy to be part of the developed core, and enhance its status to that of a world city. Despite barriers, some positive results have been observed. In terms of IT's spatial effect, it is largely overshadowed by the predeterministic long-term plans of the state's powerful planning authority.
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  • 167
    ISSN: 1573-5036
    Keywords: growth ; porewater ; salt marshes ; silicon ; Spartina ; tissue concentrations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Analysis of silicon concentrations of various halophytes from salt marshes in the S.W. Netherlands shows that the silicon concentration of Spartina anglica (Gramineae) is relatively high. To study the influence of dissolved Si concentrations on growth and plant tissue concentrations of S. anglica, silicic acid was introduced into the sediment of natural patches of this halophyte occurring on a tidal flat. This resulted in a strong increase in dissolved silicon levels in the sediment porewater. In addition, S. anglica was cultured in the laboratory on nutrient solution enriched with dissolved silicon. Neither in the field nor in the laboratory experiment did the increased silicon levels have effects on shoot growth or led to consistent increases in the silicon concentration of plant tissues. Finally, different S. anglica stands in a number of salt marshes around the Oosterschelde basin were examined on sediment porewater silicon concentrations and silicon plant tissue concentrations. Sediment porewater silicon levels ranged between 36 and 554 μM. The average silicon concentration of the shoots from the various populations ranged between 3.52 and 11.73 mg/g DW. Consistent with the results of the field and laboratory experiments, there was no correlation between porewater silicon concentrations and average shoot length at the different sites, nor between porewater silicon concentrations and shoot silicon levels. Apparently, vegetative growth and tissue silicon concentrations of S. anglica do not respond to concentrations of dissolved Si in the range to which the plants were exposed naturally or experimentally in the field and in the laboratory (ca. 15–550 μM). In the data set pertaining to the different S. anglica field populations, however, negative correlations were observed between the average number of leaves per shoot at the different locations and the total silicon content of the 4th and 2nd leaves and of the entire shoots. The possible mechanisms explaining differences in tissue Si concentrations are discussed.
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    Hydrobiologia 398-399 (1999), S. 91-100 
    ISSN: 1573-5117
    Keywords: Polysiphonia setacea ; Rhodophyta ; culture ; growth ; biogeography ; Mediterranean Sea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The red alga Polysiphonia setacea Hollenberg (=Womersleyella setacea (Hollenberg) R. Norris), described originally from the Hawaiian Islands and later reported for other tropical localities, has recently become widespread in the Mediterranean. In several localities it forms a dense, almost monospecific turf but, despite its abundance, it appears to reproduce only vegetatively; neither sporangia nor sexual reproductive structures have thus far been found. In order to elucidate its life history, plants were cultured in a variety of conditions of temperature, daylength and photon irradiance, and the upper thermal limit was also determined. Isolates of P. setacea grew well in culture, but were more tufted and branched than wild plants. No reproduction by spores or gametes was observed and only an unusual form of vegetative regeneration was found. Some pericentral cells became darker and larger than the others and produced proliferations from which new plants arose. Plants grew best at 15 and 20 °C, and relatively poor growth was observed at 10 and 25 °C. At 20 °C growth was better in long days than in short days. The upper thermal limit was 28 °C, and plants were able to tolerate a temperature as low as 5 °C for 4 weeks without any damage. These observations show that P. setacea is well adapted to the environmental conditions of the western Mediterranean, and there is good agreement with the phenology of the species in the wild. Although this entity has been treated as an introduction from a tropical area, our results suggest that the Mediterranean entity would not be able to survive or grow in surface waters of tropical areas whence this species has been reported. However, the occurrence of thermal ecotypes in P. setacea is a possibility and further studies, based perhaps on molecular data, are necessary to assess the origin of the Mediterranean populations.
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    Hydrobiologia 398-399 (1999), S. 291-297 
    ISSN: 1573-5117
    Keywords: Porphyra ; conchocelis ; aquaculture ; growth ; Alaska ; nori
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Experiments were performed to determine the range and optima of environmental conditions under which indigenous species of Alaskan Porphyra can grow. Growth of the conchocelis phase of Porphyra abbottae, P. torta and P. pseudolinearis was measured, when cultured in enriched media, under long days (16 h light) and varying conditions of irradiance (20, 40, 80 and 160 μmol photons m−2 s−1), temperature (7, 11, 15 and 19 °C) and salinity (5, 10, 20, 30 and 40‰). Optimal growth (7.6% increase in volume day−1) of P. abbottae occurred at 11 °C, 80 μmol photons m−2 s−1 and 30‰. Porphyra torta grew best (6.5% day−1) at 15 °C, 80 μmol photons m−2 s−1 and 30‰. Porphyra pseudolinearis generally had higher growth rates than the other two species with optimal growth (8.8% day−1) occurring at 7 °C, 160 μmol photons m−2 s−1 and 30‰. For all three species salinity between 20 and 40‰ had little effect on growth, but there was virtually no growth at salinity of 10‰ and below. Irradiances between 20 and 160 μmol photons m−2 s−1 generally had little effect on growth rates. However, growth of P. abbottae increased with irradiance at 7 °C but was inhibited at irradiances over 40 μmol photons m−2 s−1 at 15 °C and higher. Porphyra torta also showed growth inhibition at 15 °C and higher irradiances. Porphyra pseudolinearis appeared to be the most robust species with respect to tolerance to extremes of salinity and irradiance.
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  • 170
    ISSN: 1573-5117
    Keywords: seaweed ; morphology ; seasonal variation ; growth ; phenotypic modulation ; kelp ; Undaria pinnatifida
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Undaria pinnatifida (Harvey) Suringar is currently divided into two morphological forms, f. typica Yendo. and f. distans Miyabe & Okamura. The objective of this study was to determine the effects of seasonal variation in growth rate on the morphology of U. pinnatifida, and to define the form of U. pinnatifida growing in Otago Harbour, New Zealand. Morphological variables (stipe length, blade length, blade width, sporophyll length and degree of blade incision), growth rates (frond, blade and stipe) and blade erosion were measured each month from August 1993 to February 1995, and compared using correspondence analysis. Variation in the morphology of U. pinnatifida was largely accounted for by varying growth rates. Definition of the form of U. pinnatifida growing in Otago Harbour is equivocal because morphological characteristics of both f. typica and f. distans were exhibited at different times of the year.
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  • 171
    ISSN: 1573-5117
    Keywords: Gracilaria ; growth ; irradiance ; plant growth regulators ; salinity ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Growth and tolerance of Gracilaria vermiculophylla (Ohmi) Papenfuss from Shikoku Island were investigated under a variation of temperature (5–30 °C), salinity (5–60‰), and photon irradiance (20–100 μmol photons m−2 s−1) in unialgal culture. G. vermiculophylla showed wide tolerances for all factors tested, characterizing a euryhaline and eurythermal species. Two clones, one of a tetrasporophyte and the other of a female gametophyte, showed different growth rates, attributable to the difference either in phase or in genotype. The optimum temperature for the growth of the tetrasporophyte clone was 15–25 °C while that of the gametophyte clone was 20–30 °C. Maximum growth of both phases was observed at 80–100 μmol m−2 s−1. G. vermiculophylla presented higher growth rates in low salinities (15–30‰). Tissue cultures were established in solid ASP 12-NTA medi um supplemented with plant growth regulators (PGR), 0.5% agar, 1.0% sucrose and 0.5% inositol. Effects of two auxins (indole-3-acetic acid (IAA), and 2,4-dichlorophenoxyacetic acid (2,4-D)), and one cytokinin (6-benzylaminopurine (BA)) were tested in concentrations ranging from 0.1 to 10.0 mg l−1. Growth of apical segments was significantly stimulated by the majority of treatments supplemented with PGR, while maximum growth of calluses was observed in treatments with low concentration of auxins or BA (1.0 mg l−1). All treatments supplemented with PGR significantly promoted the growth of intercalary segments, except for IAA (1.0 mg l−1) in combination with BA (1.0 mg l−1). Growth of calluses originating from intercalary segments was observed in treatments with IAA (0.1 mg l−1), 2,4-D (10.0 mg l−1) or IAA (1.0 mg l−1) in combination with BA (0.1 mg l−1). Tr eatments with high concentration of IAA and BA (10.0 mg l−1) were lethal for apical and intercalary segments. These results show that auxin and cytokinin play a regulatory role on the growth of G. vermiculophylla in tissue culture. Furthermore, results on the effects of temperature, salinity and irradiance indicate that G. vermiculophylla could be cultivated in brackish temperate environments with potential for economic purposes and for pollution management.
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  • 172
    ISSN: 1573-2932
    Keywords: Ponderosa pine ; elevated CO2 ; growth ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Biochemical and gene expression changes in response to elevated atmospheric CO2 were investigated in five maternal half-sibling breeding families of Ponderosa pine. Seedlings were grown in a common garden located at Lawrence Livermore National Laboratory, in open-topped chambers (OTC) for two years. Chamber atmospheres were maintained at ambient, ambient + 175 μL L-1, CO2, or ambient + 350 μL L-1CO2. Growth measurements showed significant increases in stem volumes and volume enhancement ratios in three of the five families studied when grown under elevated CO2. Biochemical and gene expression studies were undertaken to gain a mechanistic understanding of these phenotypic responses. Biochemical studies focused on sucrose phosphate synthase (SPS) specific activities at increase CO2 levels. Kinetic evaluations of SPS showed an increase in VMax. Specific SPS probes revealed increases in the transcriptional levels of one SPS gene with exposure to increasing CO2. RT-PCR differential gene displays showed that overall only a small fraction of visualized gene transcripts responded to elevated CO2 (8-10%). There were also significant differences between the gene expression patterns of the different families, some of which correlated with alterations in growth at elevated CO2 levels.
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    Photosynthetica 36 (1999), S. 259-266 
    ISSN: 1573-9058
    Keywords: chlorophyll fluorescence ; fern ; fertilization ; growth ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plants grown at low irradiance were fertilized with 0, 60, and 600 g m-3 NH4NO3 once every fortnight. Plants treated with high N concentrations showed an increased growth, producing longer and broader fronds with larger areas, and were darker green in colour. Nitrogen also increased the content of chlorophyll (Chl) and carotenoids per leaf area unit. Different N treatments did not affect the photosynthetic efficiency of photosystem 2, as reflected by the high values of Chl fluorescence kinetics Fv/Fm, ranging between 0.81 to 0.84, and Fv/F0 of 4.30 to 5.10. An increase in photochemical quenching (qP), accompanied by a decrease in non-photochemical quenching (qN), was observed in sporophytes fertilized with increased concentrations of NH4NO3. Nitrogen availability allowed sporophytes of Acrostichum aureum to become more established under natural condi tions.
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    Photosynthetica 36 (1999), S. 597-599 
    ISSN: 1573-9058
    Keywords: biomass ; chlorophyll ; detoxification ; growth ; Hill reaction activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In Abelmoschus esculentus L. uniconazole brought about a marked decrease in cadmium-induced loss of chlorophyll and Hill reaction activity, but it did not completely prevent cadmium toxicity.
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    Water, air & soil pollution 114 (1999), S. 13-25 
    ISSN: 1573-2932
    Keywords: biomass ; cement dust ; growth ; partitioning ; spruces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Two-year-old seedlings of Picea abies L., P. glauca L. and P. mariana L. were planted in a sample plot subjected to high concentrations of cement dust and in an unpolluted (control) area in 1990. In 1994, the six-year-old trees were dug up in the pre-bud-break period for morphological assessment. Comparison of biomass formation and the lengths of different organs of the trees showed that alkalization of the growth substrate (pH 8.1) and the high level of dust pollution load (600–2400 g m-2yr-1) are serious factors inhibiting height growth by 61%, the length of shoots and needles by 75 and 28%, respectively, and the dry weights of roots by 88%, stems by 90%, shoots by 91% and needles by 55%. The ratio of roots to shoots dry weights increased in all species of spruces under stress. P. mariana was the most sensitive to dust pollution impact and alkalization of the environment.
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    Water, air & soil pollution 116 (1999), S. 151-197 
    ISSN: 1573-2932
    Keywords: air quality ; Physiology ; growth ; biotic and abiotic interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The perceived health of forest ecosystems over large temporal and spatial scales can be strongly influenced by the frames of reference chosen to evaluate both forest condition and the functional integrity of sustaining forest processes. North American forests are diverse in range, species composition, past disturbance history, and current management practices. Therefore the implications of changes in environmental stress from atmospheric pollution and/or global climate change on health of these forests will vary widely across the landscape. Forest health surveys that focus on the average forest condition may do a credible job of representing the near-term trends in economic value while failing to detect fundamental changes in the processes by which these values are sustained over the longer term. Indications of increased levels of environmental stress on forest growth and nutrient cycles are currently apparent in several forest types in North America. Measurements of forest ecophysiological responses to air pollutants in integrated case studies with four forest types (southern pine, western pine, high elevation red spruce, and northeastern hardwoods) indicate that ambient levels of ozone and/or acidic deposition can alter basic processes of water, carbon, and nutrient allocation by forest trees. These changes then provide a mechanistic basis for pollutant stress to enhance a wider range of natural stresses that also affect and are affected by these resources. Future climatic changes may ameliorate (+ CO2) or axacerbate (+ temperature, + UV-B) these effects. Current projections of forest responses to global climate change do not consider important physiological changes induced by air pollutants that may amplify climatic stresses. These include reduced rooting mass, depth, and function, increased respiration, and reduced water use efficiency. Monitoring and understanding the relative roles of natural and anthropogenic stress in influencing future forest health will require programs that are structured to evaluate responses at appropriate frequencies across gradients in both forest resources and the stresses that influence them. Such programs must also be accompanied by supplemental process -oriented and pattern -oriented investigations that more thoroughly test cause and effect relationships among stresses and responses of both forests and the biogeochemical cycles that sustain them.
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  • 177
    ISSN: 1573-2932
    Keywords: Genetic variability ; elevated CO2 ; climate change ; pine ; conifer ; photosynthesis ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Genetic variability can have profound effects on the interpretation of results from elevated CO2 studies, and future forest management decisions. Information on which varieties are best suited to future atmospheric conditions is needed to develop future forest management practices. A large-scale screening study of the effects of elevated CO2 on 15 half-sibling sources of genetically superior ponderosa pine (Pinus ponderosa Dougl ex P. Laws.) is presented. These sources represent multiple elevations and latitudes throughout California. Among-provenance variability in the effects of elevated CO2 on gas exchange and growth, and their correlation with geographic origin were investigated in ponderosa pine seedlings subjected to ambient or elevated CO2 concentrations (525 μmol mol-1 CO2, and 700 μmol mol-1 CO2) for more than two years in open-top chambers. Substantial among-provenance variability in growth response to elevated CO2 was evident, with 8 sources demonstrating no significant growth response to elevated CO2 while 7 sources responded positively. For all sources, elevated CO2 increased photosynthesis (ranging from 19% increase at 525 μmol mol-1 CO2 to 49% increase at 700 μmol mol-1 CO2). A modest correlation existed between geographic origin and above ground growth response to elevated CO2.
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    Water, air & soil pollution 116 (1999), S. 99-110 
    ISSN: 1573-2932
    Keywords: Nitrogen ; Fagus sylvatica ; Picea abies ; nutrition ; growth ; parasite attack
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract In permanent observation plots across Switzerland, nitrogen (N) concentration in the foliage of mature beech has increased by 15% and phosphorus (P) concentrations in beech and mature Norway spruce decreased by 12 and 13% respectively between 1984 and 1995, leading to increased N:P ratios. Modelled N deposition was correlated with stem increment in both beech and spruce, with indications of P limitation in some beech plots. Experimental application of 0-160 kg N ha-1〉 yr-1〉 over four to five years caused nutrient imbalances in various afforestation plots comparable to those observed in the permanent observation plots. The changes in the trees caused by N treatment led to increased attacks by parasites such as Apiognomonia errabunda, Phomopsis sp., Phyllaphis fagi in beech and Botrytis cinerea, Sacchiphantes abietis and Cinara pilicornis in Norway spruce. The results suggest current N deposition in Switzerland induces significant changes in the forest ecosystem.
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    Interface science 7 (1999), S. 285-295 
    ISSN: 1573-2746
    Keywords: computer simulation ; growth ; energy dissipation ; grain boundaries ; triple nodes (Interfaces) ; triple line (Interfaces)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Vertex dynamics models in two and three dimensions are applied to study grain growth including a limited mobility of triple points (two dimensions) and triple lines (three dimensions). A recent experiment on a triple node is used to validate the proposed model. The reduced triple node/line mobility in the case of a polycrystalline sample is shown to influence both, the grain growth kinetics and the grain size distribution function.
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  • 180
    ISSN: 1573-2746
    Keywords: polycrystal ; ionic ; oxide ; molecular-dynamics ; simulation ; growth ; thin film ; structure ; characterization ; grain boundary
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A simulation methodology for the synthesis of polycrystalline, ionic thin films is developed. The method involves the preparation of a polycrystalline substrate onto which a thin film is subsequently grown by crystallization from the melt. A detailed structural analysis of a textured sixteen-grain FeO film, with a grain size of approximately 4.7 nm, shows that the interiors of the grains are almost perfect single crystals with only a very few vacancies and no interstitials. The grains are delineated by 〈001〉 tilt grain boundaries; as expected, the low-angle grain boundaries in the film consist of arrays of dislocations, while the high-angle grain boundaries are relatively narrow and well ordered.
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    Evolutionary ecology 13 (1999), S. 395-409 
    ISSN: 1573-8477
    Keywords: body size ; development ; Drosophila melanogaster ; dung flies ; growth ; life history ; resource limitation ; Scathophaga stercoraria ; seasonality ; Sepsis cynipsea ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Growth responses to temperature and resource limitation in three dipteran species with similar life histories were compared. With respect to current life history theory, two points are raised. First, growth rate in real time increased steeply with temperature in all species, following the standard pattern. However, when expressed in physiological time growth rate increased as temperature decreased in the yellow dung fly Scathophaga stercoraria, remained approximately constant in Sepsis cynipsea, and increased in Drosophila melanogaster. These responses can be understood as adaptations to climate and seasonality. It is concluded that some patterns of adaptation may be more easily interpreted if, and some may even go undetected unless, they are analysed in physiological time. Second, a decrease in body size, development rate and growth rate when resources are limited is believed to be nearly universal and generally predicted by life history models. Despite their similar life histories, the three species investigated showed qualitatively different growth responses to larval food shortage. At unlimited resources, yellow dung flies showed the fastest initial larval body mass gain per unit time, while those of S. cynipsea and D. melanogaster were lower and about equal. The period of no body mass gain at the end of larval development was longest in S. stercoraria and shortest in S. cynipsea. When facing resource limitation, S. stercoraria emerged smaller but earlier (thus nearly maintaining their growth rate), S. cynipsea smaller after the same development period, and D. melanogaster smaller and later (showing reduced and much reduced growth, respectively). It is concluded that whether growth really slows when resources are limited depends on the precise ecological circumstances of the species in question. More refined models, particularly those where mortality costs are independent of time, and more experiments are necessary to account for the variation in growth and size and age at maturity present in nature.
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  • 182
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    Evolutionary ecology 13 (1999), S. 709-719 
    ISSN: 1573-8477
    Keywords: Anthocharis cardamines ; aposematism ; Lepidoptera ; palatability ; Pieridae ; Pieris brassicae ; P. napi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract It has been suggested that the white coloration of Pieridae butterflies is a warning signal and therefore all white Pieridae could profit from a mimetic resemblance. We tested whether green-veined white (Pieris napi) and orange-tip (Anthocharis cardamines) butterflies benefit from white coloration. We compared their relative acceptability to wild, adult pied flycatchers (Ficedula hypoleuca) by offering live A. cardamines and P. napi together with two non-aposematic butterflies on the tray attached to birds' nesting boxes. Experienced predators equally attacked white and non-white butterflies, and the order of attack among the Pieridae was random. If anything, there was a slight indication that the female A. cardamines was the least favoured prey. Since birds did not avoid white coloration, we compared the palatability of these two species against known palatable and unpalatable butterflies by presenting them to great tits (Parus major). Pieris brassicae, which has been earlier described as unpalatable, was also included in the palatability test. However, there were no significant differences in the palatability of the butterflies to birds, and even P. brassicae was apparently palatable to the great tits. Our results do not unambiguously support the hypothesis that the white coloration of Pieridae would signal unpalatability. Nevertheless, in our last experiment, pied flycatchers often rejected or left untouched free flying P. napi and A. cardamines. This suggests that other features in a more natural situation, such as the agile flight pattern or odours might still make them unprofitable to birds.
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  • 183
    ISSN: 1573-8264
    Keywords: aminopeptidase ; carboxypeptidase ; growth ; ×Haynaldoticum sardoum ; proteinase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of 50 to 200 mM NaCl on two lines (CP with solid stem and CV with hollow stem) of ×Haynaldoticum sardoum was studied. NaCl significantly reduced root and shoot fresh and dry masses, root length and less markedly shoot length of CP and CV plants. The sodium accumulated in the leaves in relation to the concentration of NaCl and length of the treatment; CP leaves contained twice as much sodium as CV leaves. The leaf chlorophyll a/b ratio was not affected by NaCl. NaCl decreased the leaf water and osmotic potentials. The pressure potential increased due to the increased concentration of dissolved solutes in the leaf, particularly sodium. The proteinase and exopeptidase activities increased during NaCl treatment.
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  • 184
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    Biologia plantarum 42 (1999), S. 631-636 
    ISSN: 1573-8264
    Keywords: allocation pattern ; 14C-sucrose ; Cecropia pachystachya ; growth ; Hymenaea courbaril ; Myroxylon peruiferum ; Schizolobium parahyba ; tropical tree species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Carbon translocation was affected by shade in different tropical tree species differing in successional status and degree of shade tolerance. Plants of the early-successional shade-intolerant species Cecropia pachystachya and Schizolobium parahyba and of the late-successional shade-tolerant species Myroxylon peruiferum and Hymenaea courbaril were grown under full sun (FS) and natural shade treatments (NS) and assessed for [14C]-sucrose translocation. Most of the 14C was retained in the fed leaf after a 24 h translocation period. Under FS, the growing apical part of the plant was the most intense sink for most species. Shade affected growth and sink intensity differently in early and late successional species. Growth was more markedly affected in the early species. Whereas these continued to invest carbon into the growing apical part of the plant under shade conditions, the late successional species invested relatively more into other sinks.
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  • 185
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    Entomologia experimentalis et applicata 86 (1998), S. 89-96 
    ISSN: 1570-7458
    Keywords: spruce budworm ; amino acids ; chemosensilla ; electrophysiology ; gustation ; Lepidoptera ; Tortricidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An electrophysiological approach was used to record the responses of maxillary styloconic sensilla of fourth- and sixth-instar larvae of the spruce budworm Choristoneura fumiferana to 14 amino acids. One cell in the lateral styloconic sensillum was identified as an amino acid-sensitive neuron. All of the amino acids tested, except l-proline and l-arginine, were detected by this cell. Arginine did not evoke a response from either the medial or lateral styloconic sensilla. Proline evoked responses from a cell in the medial styloconic sensillum. It is known from previous behavioural work that l-proline is a phagostimulant and l-valine inhibits feeding in Choristoneura; we thus further characterized the responses to these two amino acids. For both instars, l-proline was detected as low as 0.001 mmol/l and the maximal response was at 50 mmol/l. Stimulation of fourth- and sixth-instar larvae with l-valine showed that the maximum firing frequency was obtained at 1 mmol/l. Above and below this concentration, firing frequency decreases. Sensory responses to the amino acids stimuli did not correlate with known behavioral responses to similar stimuli.
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    Entomologia experimentalis et applicata 86 (1998), S. 331-336 
    ISSN: 1570-7458
    Keywords: adult diet ; fecundity ; larval phase ; Lepidoptera ; Noctuidae ; Spodoptera exempta
    Source: Springer Online Journal Archives 1860-2000
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  • 187
    ISSN: 1570-7458
    Keywords: attraction ; Allium ; EAG ; generations ; green leaf volatiles ; Hyponomeutoidea ; Lepidoptera ; leek moth ; walking behaviour ; olfactory responsiveness ; rearing conditions ; sensitivity variations ; sulphur volatiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three strains of the leek moth, Acrolepiopsis assectella, were compared: two reared on leek leaves (Allium porrum) for which generations 10 to 12 (L) or generation 2 (G2) were used, the third one (D) reared on an artificial diet incorporating leek powder, for which generation ca 140 was examined. The walking behaviour (L and D) and the antennal responsiveness (L, G2 and D) were compared between individuals subjected to leek leaf odour and 4 volatiles identified in these leaves: 2 specific sulphur ones (dipropyl thiosulphinate and dipropyl disulphide) and 2 ‘general green leaf volatiles’ (cis-3-hexen-1-ol and trans-2-hexen-1-ol). The walking behavioural thresholds to leek odour, dipropyl thiosulphinate, and cis-3-hexen-1-ol were lower for L than for D females. The chemoanemotactic responses to dipropyl disulphide are almost identical, and trans-2-hexen-1-ol has nearly no activity. Accordingly, the olfactory sensitivity (assessed by EAG) to sulphur volatiles and cis-3-hexen-1-ol was higher for L and G2 females than for D ones. The threshold for thiosulphinate was at a concentration (v/v) of 4 × 10-4 M/l for D females, 4 × 10-5 M/l for L females and 4 × 10-8 M/l for G2 ones. It may be hypothesised that laboratory rearing conditions cause variations in the thresholds of antennal sensitivity and walking behaviour to specific and more efficient leek volatiles. This may result from the selective pressures experienced by the larvae reared in the laboratory.
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    Entomologia experimentalis et applicata 86 (1998), S. 313-318 
    ISSN: 1570-7458
    Keywords: Ostrinia palustralis ; sex pheromone ; identification ; (E)-11-tetradecenyl acetate ; (Z)-11-tetradecenyl acetate ; Lepidoptera ; Pyralidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract By means of gas chromatography with electroantennographic detection, gas chromatography-mass spectrometry and a series of bioassays, (E)-11-tetradecenyl acetate (E11-14:OAc) and (Z)-11-tetradecenyl acetate (Z11-14:OAc) at a ratio of 99:1 were identified as female sex pheromone components of Ostrinia palustralis. The average amounts of E11- 14:OAc and Z11-14:OAc in a single sex pheromone gland were 37.2±24.4 ng and 0.3±0.2 ng, respectively. In a wind-tunnel bioassay, the binary blend of E11- and Z11-14:OAc elicited the same male behavioral responses as did virgin females.
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  • 189
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    Entomologia experimentalis et applicata 88 (1998), S. 1-7 
    ISSN: 1570-7458
    Keywords: Lobesia botrana ; Lepidoptera ; Tortricidae ; fungus ; Botrytis cinerea ; attraction ; olfaction ; gustation ; olfactometer ; larval behaviour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In vineyards, larvae of Lobesia botrana (Denis and Schiffermüller) (Lepidoptera: Tortricidae) and Botrytis cinerea (Persoon: Fries) often occur together. This study was carried out to establish whether first instar larvae are attracted by the fungus. Olfactory and gustatory responses of larvae to the fungus were investigated on grape berry clusters (Vitis vinifera) and in two types of olfactometer. In clusters infected partly by B. cinerea, 82% of first instar larvae settled where berries had been infected. In the olfactometers, first instar larvae detected and discriminated between two tested foods. They significantly preferred synthetic media or grape berries (cv., 'Alphonse Lavallée' or 'Cabernet Sauvignon') infected with B. cinerea to non-infected controls. The olfactory response led to 62.2% to 72.6% of the first instar larvae attracted by the fungus and the association of olfactory with gustatory responses resulted in similar rates (64.1% to 81.6%). So, the larvae were most attracted by synthetic media or grape berries infected by the fungus as compared to the controls. Olfaction appears to be the main sense involved in this attraction process.
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  • 190
    ISSN: 1570-7458
    Keywords: Lepidoptera ; Gelechiidae ; Pectinophora gossypiella ; cotton ; pheromone ; wind tunnel ; attraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The mechanisms by which the application of formulated pheromone interferes with mating in the pink bollworm moth (PBW), Pectinophora gossypiella were examined in 0.4 ha cotton fields using high-dose (78 mg A.I.) sealed polyethylene dispensers. Walk-in, field wind tunnels 6.2 m long were placed over two rows of cotton. Treatments consisted of a control, a tunnel in a field free of disruptant formulation; a 3-rope treatment, in which the field was free of pheromone but one of the cotton rows in the wind tunnel was treated with 3 PBW ropes; and a rope-grid treatment, in which the field was treated with PBW ropes at the standard density of 1000 ha−1 and one of the cotton rows inside the wind tunnel was treated with 3 PBW ropes. We released marked males into the tunnels near sunset or held them in field cages for 24 h prior to assay. Two pheromone traps at the tunnel's upwind end monitored the ability of males to locate point sources of pheromone. In the 3-rope tunnel, traps placed upwind of the cotton row treated with disruptant pheromone captured far fewer males than those placed upwind of the untreated cotton row. In the tunnel situated in the centre of the rope-gridded field, very few males were caught in traps in both rows, indicating a camouflage of the pheromone plumes from the traps by the background of airborne disruptant drawn into the tunnel from the field. Activity of moths near the synthetic pheromone sources was video-recorded. Males oriented to, landed on or near, and walked on or near, PBW ropes, indicating competition between pheromone sources as a mechanism of mating disruption. Most males visiting PBW ropes became quiescent or disappeared from the field of view after a few minutes, suggesting a habituation/adaptation of response. The rhythm of attraction of males held in the field for 24 h before release was comprised of a small peak of activity near 2000 h, with the majority of attraction between 2300 and 0300 h. Much of the attraction before 0100 appears to be an advancement of the male's normal diel rhythm, caused by the presence of disruptant. Together these findings indicate that mating disruption of pink bollworm using the PBW ropes is achieved by a combination of mechanisms: a camouflage of natural plumes, competition between pheromone sources, habituation, and some advancement of the male's rhythm of response.
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  • 191
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    Entomologia experimentalis et applicata 87 (1998), S. 209-209 
    ISSN: 1570-7458
    Keywords: Chrysodeixis chalcites ; EAG ; electroantennography ; electrophysiology ; greenhouse cultures ; Lepidoptera ; mating disruption ; Noctuidae ; paprika ; pheromone distribution ; sex pheromones ; sweet pepper
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mating disruption using pheromone dispensers is an emerging insect control method in greenhouses in the Netherlands. For routine measurements of the pheromone level in greenhouses a portable and compact device based on electroantennography (EAG) was developed. The instrument was tested in different greenhouses under varying conditions using antennae of males of the noctuid moth Chrysodeixis chalcites. Relative differences in pheromone concentration between greenhouses loaded with pheromone dispensers and control greenhouses could be measured in a reliable way. Changes in pheromone concentration were clearly demonstrated by measurements during the growing season. The advantages of the instrument presented over previously described devices are its true portableness, ease of use and uncomplicated measuring principle. The device can be operated routinely by non-experts, and many measurements can be made in a short period of time. Preliminary outdoor measurements using antennae of male Cydia pomonella as sensor showed the potential of the instrument for use in the open field. The portable EAG sensor described here may provide a practical means for monitoring pheromone concentration and dispersal from dispensers applied for mating disruption.
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    Entomologia experimentalis et applicata 88 (1998), S. 101-107 
    ISSN: 1570-7458
    Keywords: sex pheromone ; pheromone-based mating disruption ; Spilonota ocellana ; Lepidoptera ; Tortricidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The potential for pheromone-based mating disruption of eye-spotted bud moth (ESBM), Spilonota ocellana (Denis & Schiffermüller) (Lepidoptera: Tortricidae), in apple orchards in the Okanagan Valley of British Columbia was examined in small-plot trials. In a preliminary experiment, treatment of the orchard atmosphere with a 99:1 blend of Z8-tetradecenyl acetate (Z8-14:OAc) and Z8-tetradecenyl alcohol (Z8-14:OH) completely inhibited captures of male ESBM in pheromone-baited traps, but treatment with Z8-14:OAc alone did not. Therefore, all subsequent trials used the two-component blend as a disruptant. Mean catches of male ESBM in traps baited with 0.1, 1, 10, or 20 mg of 99:1 Z8-14:OAc and Z8-14:OH were significantly reduced (81–97%) in pheromone-treated plots relative to similar traps placed in control plots. In both control and treated plots, there was a significant positive relationship between trap bait dose and trap catch. In pheromone-treated plots, this suggests that high doses of trap baits over-ride the camouflage effect of disruption or overcome the effect of sensory adaptation and habituation. The number of virgin-female baited traps capturing at least one male ESBM was reduced by 96%, and mating of virgin females on mating tables was reduced by 95% in plots treated with the two-component pheromone. The total amount of Z8-14:OAc released from pheromone disruption dispensers during the latter field trials was estimated to average 6.4 g ha-1 over 11 days or 26.4 mg ha-1 h-1. The low levels of Z8-14:OH released from disruption dispensers could not be estimated by GC analysis. Dispensers loaded with a 99:1 blend of Z8-14:OAc and Z8-14:OH were highly attractive to males in baited traps, indicating that they have the potential to induce false trail following.
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    Journal of insect behavior 11 (1998), S. 507-538 
    ISSN: 1572-8889
    Keywords: monarch butterflies ; Danaus plexippus ; Danainae ; Lepidoptera ; Nymphalidae ; oviposition behavior ; Asclepias ; Asclepiadaceae ; chemoreceptors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The monarch butterfly, Danaus plexippus L., oviposits mainly on plants in the Asclepiadaceae, particularly within the genus Asclepias. We studied postalightment oviposition behavior of monarch females on three host species—Asclepias curassavica, A. incarnata , and A. tuberosa. After landing on the host, they used their forelegs, midlegs, and antennae to assess plant suitability. When these appendages were examined by scanning electron microscopy, contact chemoreceptor sensilla were found. In choice tests, A. incarnata was most preferred, while A. tuberosa was least preferred. However, the use of appendages varied for the different host species. Antennae were most frequently used during post-alightment behavior on A. curassavica, whereas forelegs were used more often on A. incarnata, and all three appendages were used extensively on A. tuberosa. Use of the midlegs was generally followed by use of the antennae. Tasting with either forelegs or antennae apparently may lead to egg laying on some host species. Rupture of the plant surface by midleg spines was also observed. The behavior and host preference of individual females varied significantly and may reflect differences in receptor sensitivity.
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  • 194
    ISSN: 1572-8889
    Keywords: trail following ; 5β-cholestane-3-one ; pheromone ; cooperative foraging ; social caterpillar ; bolsa ; silk ; Pieridae ; Eucheira ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The larva of the Madrone butterfly Eucheira socialis (Lepidoptera: Pieridae) secretes a trail pheromone from the ventral surface of the posterior tip of its abdomen. Caterpillars mark trails by bringing the secretory site into brief contact with the substrate during a locomotive cycle. Foragers mark most heavily when they move onto new branches and little, if at all, when they move over established trails or when they return to the communal shelter after feeding. The caterpillars make careful comparisons of alternative pathways at choice points and select newer and stronger trails over older and weaker trails. Differential marking of new and established trails during nightly forays, coupled with sensory discrimination of trails by strength and age, leads colonies to abandon old trails in favor of new trails. When applied at a rate as low as 2.5 × 10 −10 g/mm, caterpillars followed synthetic trails prepared from 5β-cholestane-3-one, a trail pheromone previously reported from the tent caterpillars (Malacosoma spp.). Although both Eucheira and Malacosoma mark with the tip of the abdomen and have near-identical sensitivites to 5β-cholestane-3-one, our study shows that Eucheira employs a relatively unsophisticated system of trail-based communication and does not recruit to food. The trail-based communication system of Eucheira appears to represent an early stage in the evolution of cooperative foraging that is derived from, and motivationally linked to, conflict behavior.
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  • 195
    ISSN: 1572-8889
    Keywords: radar tracking ; harmonic radar ; foraging flight ; Lepidoptera ; Agrotis segetum
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    Journal of insect behavior 11 (1998), S. 343-359 
    ISSN: 1572-8889
    Keywords: Agrotis segetum ; Lepidoptera ; Noctuidae ; turnip moth ; reproduction ; sex ratio ; reproductive capacity ; sexual selection ; mate discrimination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We investigated the lifetime mating potential and the reproductive behavior of male and female turnip moths Agrotis segetum (Schiff.) under field and laboratory conditions. The sex ratio was 1 : 1 in a lab-reared population as well as in two wild populations. Males were capable of mating repetitively a relatively large number of times (mean of 6.7 ± 2.7 matings) when given access to new virgin females throughout their lifetimes. Females seldom mated more than once (mean ± 1.3 ± 0.6 matings), indicating a male-biased operational sex ratio. The mean potential lifetime mating was five times higher in males, while the coefficient of variance was lower in males. There was no differences in longevity between animals that were allowed to mate and animals not allowed to mate, indicating no direct costs or benefits of mating in physiological terms. In males, the number of matings was positively correlated with longevity, but this was not the case in females. Nor was there a correlation between the number of female matings and the number of fertilized eggs. There was a negative correlation between the number of eggs fertilized and the number of times males had previously mated, indicating that male ejaculates were limited. Male spermatophore size also decreased with number of achieved matings. Laboratory-reared females attracted males in the field throughout their lifetimes, with a peak at 3–7 days of age. Wild males, allowed to choose between pairs of caged females in the field, were attracted in equal numbers to females of different ages. Females did not show any mate-rejection behavior in the field. They mated with the first male that courted them. No incidence of mate replacement by males arriving later to already courted females were recorded.
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    Journal of population economics 11 (1998), S. 273-291 
    ISSN: 1432-1475
    Keywords: JEL classification: F22 ; O3 ; J61 ; Key words: Immigration ; assimilation ; growth ; diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Sociology , Economics
    Notes: Abstract. This paper analyzes the welfare effects of immigration and its subsequent effect on ethnic diversity in a model featuring human capital spillovers which depend on the degree of ethnic heterogeneity, variation rates of time preference across individuals and endogenous levels of immigration and assimilation. In the model, an increase in ethnic diversity reduces the spillovers effect for the majority. Nonetheless, immigration can be welfare improving for the majority ethnic group even if it increases the degree of diversity as long as it raises the average human capital level and/or growth rate by increasing the proportion of people with low rates of time preference. However, if an economy is too homogenous, it will not be able to attract immigrants. Finally, if the level of immigration is not too high, then immigration also raises the net benefits to assimilation which leads to a more homogenous economy.
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    Journal of population economics 11 (1998), S. 517-534 
    ISSN: 1432-1475
    Keywords: Key words: Fertility ; mortality ; growth ; JEL classification: J13 ; O41
    Source: Springer Online Journal Archives 1860-2000
    Topics: Sociology , Economics
    Notes: Abstract. Economic and demographic outcomes are determined jointly in a choice-theoretic model of fertility, mortality and capital accumulation. There is an endogenous population of reproductive agents who belong to dynastic families of overlapping generations connected through altruism. In addition to choosing savings and births, parents may reduce (infant) deaths by incurring expenditures on health-care which is also provided by the government. A generalised production technology accounts for long-run endogenous growth with short-run transitional dynamics. The analysis yields testable time series and cross-section implications which accord with the empirical evidence on the relationship between demography and development.
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    Journal of thermal analysis and calorimetry 52 (1998), S. 537-542 
    ISSN: 1572-8943
    Keywords: fractals ; growth ; nucleation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The author presents a model based on multi-step nucleation for the heterogeneous decompositions described by nucleation and growth phenomena, taking into account the fractal nature of the nuclei.
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    Aquaculture international 6 (1998), S. 1-17 
    ISSN: 1573-143X
    Keywords: Atlantic salmon (Salmo salar) ; Condition factor ; feeding regimes ; growth ; proximate composition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of different feeding methods on growth and slaughter traits of Atlantic salmon, Salmo salar, have been examined. Swimming activity associated with feeding was greatest amongst fish fed using automatic feeders, intermediate amongst those fed predetermined rations by hand, and least in groups of fish fed to satiation twice per day. Fish fed to satiation ate more and grew faster than did those provided with rations predicted to support high rates of growth, but the fish fed to satiation had higher feed:gain ratios than those given predetermined rations as two meals per day. The muscle tissues of the fish fed to satiation had higher lipid (%) and lower moisture (%) contents than did those of the fish fed the predetermined rations, but differences in muscle composition could be accounted for by differences in the size of the fish. Lipid was positively, and moisture negatively, correlated with fish size, whereas per cent protein and dress-out losses were little influenced by fish size and feeding regime. The fish fed to satiation were slightly heavier for a given length than those in the other groups. There was, however, no evidence that the largest fish had the highest condition factor, nor could condition factor be used to predict per cent lipid in the muscle tissue. Size variation increased in all groups of fish, and this was, in part, due to the cessation of growth of maturing individuals. Size variation was, however, most marked in the groups fed using automatic feeders, suggesting that interindividual competition for food was greatest amongst these fish.
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