Publication Date:
1985-04-05
Description:
Human T-cell lymphotropic virus type III (HTLV-III), the causative agent of the acquired immune deficiency syndrome (AIDS), was recently isolated and its genomic structure analyzed by DNA cloning methods. In the studies reported here a combined cloning and expression system was used to identify HTLV-III encoded peptides that react immunologically with antibodies in sera from AIDS patients. Cloned HTLV-III DNA was sheared into approximately 500-base-pair fragments and inserted into an "open reading frame" expression vector, pMR100. The inserted DNA was expressed in Escherichia coli transformants as a polypeptide fused to the lambda CI protein at its amino terminus and to beta-galactosidase at its carboxyl terminus. Sera from AIDS patients containing antibodies to HTLV-III were then used to screen for immunoreactive fusion proteins. Twenty clones, each specifying a fusion protein strongly reactive with AIDS serum, were identified. DNA sequence analysis indicated that the HTLV-III fragments were derived from the open reading frame DNA segments corresponding to the gag and pol gene coding regions and also the large open reading frame region (env-lor) located near the 3' end of the viral genome.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chang, N T -- Chanda, P K -- Barone, A D -- McKinney, S -- Rhodes, D P -- Tam, S H -- Shearman, C W -- Huang, J -- Chang, T W -- Gallo, R C -- New York, N.Y. -- Science. 1985 Apr 5;228(4695):93-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2983429" target="_blank"〉PubMed〈/a〉
Keywords:
Acquired Immunodeficiency Syndrome/immunology
;
Antibodies, Viral/immunology
;
Cloning, Molecular
;
DNA, Recombinant/metabolism
;
DNA, Viral/genetics
;
Deltaretrovirus/*genetics
;
Electrophoresis, Polyacrylamide Gel
;
Escherichia coli/genetics
;
Genes, Viral
;
Genetic Vectors
;
Humans
;
Viral Proteins/*genetics/immunology/isolation & purification
;
beta-Galactosidase/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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