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  • Temperature  (173)
  • Rainbow trout  (104)
  • Chemistry
  • LUNAR AND PLANETARY EXPLORATION
  • Inorganic Chemistry
  • Iranian Fisheries Science Research Institute  (273)
  • 1
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    Study on propagation and breeding of Hamoun fish (shizothoraxzarudnyi) to one gram weight in earthen pond (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25322 | 18721 | 2018-09-12 09:50:04 | 25322 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Hamoun fish, Schizothorax zarudnyi, is an indigenous species of the eastern waters of Iran, which is exclusively found in this region. However recently drought occurrence in the Chahnimeh reservoirs (a semi natural water body) making them vulnerable to extinction. As an appropriate action to address this problem and according to the 3 side contract between Italian Government – UNDP - Islamic republic of Iran Government and the Italian government financial support to reduce poverty in the Province of Sistan-Baluchestan through the reinforcement and dissemination of aquaculture activities the project was developed at the Hamoun Research Unit by the Chahbahar Fisheries Research Center to record the breeding normative of 1 g weight larvae for restocking and other researching purposes. 331 broodstocks of the indigenous species Shizothorax zarudnyi weighing 800-2450 g were collected from the Chahnimeh reservoirs in early autumn, 2006. From 5/3/2007 (the project is supported and communicated on 2010) , Ovulation was stimulated with three stimulators; pituitary extract (3-6 mg kg-1 body weight), GnRH-A (20-30 mg kg-1 body weight) and anti dopamine (10-15 mg kg-1 body weight) that was given in 2-3 doses to breeders. Of 169 injected breeders , some were injected On mid March of 2007 (12-13 ºC water temperature) responded to the injection 25% ,while the rest were injected On April of 2008 (14-16 ºC water temperature) responded to the injection 65%. In the present project of 167 breeders 82 were female and 87 male. Totally 30 female breeders released their eggs in different stages. 20 female breeders released their eggs completely, 3 breeders released half of their eggs and 7 released 1/3 of their eggs. The male breeders just injected in the final dose of hormontrapy and all were ready for releasing sperms however the ovulation in female breeders occurs between 353-428 h ºC and after the final dose of injection. Ripe eggs were stripped from the females and fertilization was done by the dry method. Fertilized eggs were transferred to veis incubators and troughs. Incubation period for eggs differs and larvae hatch out after about 910 days at an average water temperature of 12.5 °C. Maintained at 13-14 °C, complete absorption of yolk sac in Shizothorax zarudnyi larvae occurred after 5-8 days. Larvae were fed with a mixture of powdered milk and egg yolk in this stage followed by decapsulated Artemia cysts and nauplii of Artemia and then on formulated starter diets used for carps. Because the ponds were not ready, larvae were maintained in troughs for about ten days before they were transferred to two 1200 m2 earthen ponds where they reached a body weight of about 1 g. They were then handed over to the Iranian Fisheries department in the region. Larvae were fed with the starter feed SFCO in the earthen ponds. About 350 thousand larvae were stocked in two earthen ponds. Based on the results of present study and other studies we may conclude that artificial breeding in Schizothorax can be successfully achieved at 14-16 ºC in flow through systems using hormone therapy (combination of GnRha and anti dopamine) and larvae could be easily cultured in earthen ponds. However this species exhibits lower growth rates as compared to carps its high expenses could have an important role in economical feasible.
    Keywords: Aquaculture ; Iran ; Sistan and Baluchestan province ; Chahnimeh reservoirs ; Chahbahar ; Hamoun fish ; Schizothorax zarudnyi ; Breeding ; Species ; Aquaculture ; Temperature ; Female ; Eggs ; Hormontrapy ; Sperm ; Ovulation ; Larvae ; Fisheries ; Earthen ponds
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  • 2
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    Investigation on feasibility polyculture of Litopenaeus vannamei with Mugil cephalus in earthen shrimp ponds (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25324 | 18721 | 2018-09-12 09:56:53 | 25324 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This study was conducted in order to optimizing of the biological condition and enhancing of the harvest efficiency for cultured shrimps of Guater site. Experimental design was consisted of three treatments and each treatment with three replications (totally 9 ponds with 600 m^2 area for each pond). After preparing and watering of ponds, the ponds was stocked with shrimp post larves in a density of 0.007±0.001 g (or 20 numbers per m^2) per m^2. After 35 days, the fingerlings of gray mullet were released to shrimp ponds in densities of 0/100 m^2 (T1/), 2/100 m^2 (T2) and 4/100 m^2 (T3). During experiment, the physicochemical parameters of water (temperature, O_2, pH, water transparency and salinity) were measured daily for two times (morning and afternoon). Also, the nitrate, nitrite, ammonia, phosphate and silicate concentrations and BOD5 as well as chlorophyll (a), phytoplanktons and zooplanktons were measured every 15 days one time. To determine the organic values of the bottom sediment of the ponds, monthly sampling was conducted. Health status of shrimps was investigated monthly too. Growth rate, mean weight, survival value, Food conversion ratios (FCR) and total shrimp harvest (Kg) were measured after 107 days rearing in ponds. ANOVA analysis showed significant differences between treatments. The highest (212 Kg, 3533 Kg; weight mean was18.4 g for each shrimp) and lowest (187 Kg, 3116 Kg; weight mean was16.23 g for each shrimp) of harvested shrimps were yielded in T3 and T1 respectively. There was not significant differences between treatments in terms of survival rate (P〉0.05). After 107 days rearing, the highest (1.27 0.2) and lowest (1.2 0.1) of FCR were observed in T_3 and T_1 respectively. Although, there were no significant differences between T_2 and T_3 in ammonia, O_2, Total organic material, pH and BOD5, but, T_2 and T_3 had significant differences with T_1 in these parameters. In this study, 27 genus of phytoplanktons belonged to Diatoms (Bacillariophyceae), blue-green algal (Cyanophyceae) and dinoflagellates (Dinophyceae) were identified. Diatoms with 20 genus had more abundance than dinoflagellates (6 genus) and blue-green algal (1 genus). Also, 7 orders of zooplanktons including copepod, mollusks, nauplius of crustacean and rhizopoda were identified. Health investigation of T1 (without shrimp) showed more pathogenic pollution (parasites and bacteria) than in treatments with shrimp. In this regard, among bacteria, the Vibrio genus (V.Alginoliticus and V.Fluvialis) had more abundance and among parasites zoothamnium sp and Epistylis sp were identified which more abundance was for zoothamnium sp. In conclusion, our results concluded that mixed culturing of white shrimp and grey mullet in optimum densities is possible and is caused more production of shrimp.
    Keywords: Aquaculture ; Iran ; Chabahar ; Guater ; Litopenaeus vannamei ; Mugil cephalus ; Mixed culturing ; Polyculture ; Earthen ponds ; Shrimp ; Fingerlings ; Gray mullet ; Temperature ; O2 ; pH ; Water transparency ; Salinity ; Chlorophyll a ; Phytoplanktons ; Zooplanktons ; ANOVA ; Diatoms ; Bacillariophyceae ; Blue-green algae ; Cyanophyceae ; Copepod ; Investigation
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  • 3
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    A survey on environmental effects of shrimp pond culture effluents in Gwatar area (Sistan & Blouchestan) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25327 | 18721 | 2018-09-12 09:54:28 | 25327 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Present project was conducted in shrimp farm located in east of Chabahar in Sistan and Balouchestan province.Twentieth sampling stations at three locations (i.e. 2 stations in supply channel,13 stations in drainage channel and 5 stations in Gwatar Gulf) were selected to determine physical and chemical factors such as; temperature, salinity, dissolved oxygen, pH, nitrate, nitrite, ammonia, phosphate and biological parameters for example Chlorophyll a, phytoplankton and zooplankton. Sampling was conducted once a month from March 2003 until January 2004. The results revealed different bioenvironmental area in the three locations (i.e. supply channel, drainage channel and Gwatar Gulf) over the study period. Concentration of salinity ,pH , nitatr ,nitrite ,ammonia and chlorophyll a in drainage channel were more than supply channel and Gawtar Gulf. While concentation dissolved oxygen and phosphate were in drainage channel less than Gawtar Gulf.Also, sediment with small grain size (clay and silt) caused uptake phosphate and amount phosphate decreased in drainage channel. Considering with lack of environment standards in Iran shrimp effluent, data compared with others countries standard. Results showed that with considerable farm area, value of temperature, salinity, dissolved oxygen and pH were in permit range in Gwatar Gulf while concentation nitrate, phosphate and chlorophyll a were sometimes more than permit range that could cause plankton bloom. Also, Gwatar Gulf were influence monsoon. Nitate, phosphate, chlorophyll a and phytoplankton increased in gwatar Gulf.Regards with that postmonsoon was simultaneus with shrimp harvest.Therefore, increase some parameters could be caused from shrimp effluent or postmonsoon. Thirty four phytoplankton genus were identified including diatoma, dinoflagellate, cyanophyta and chlorophyta. In each area maxiumum percentage of phytoplankton were belonged to diatoma (63%) in supply channel, cyanophyta (51.7%) in drainage channel and dinoflagellate (94.5%) in Gwatar Gulf.Amount and range chemical and physical factors and plankton composition were different in drainage channel, supply channel and Gwatar Gulf. Cyanophyta could grow more in drainage channel.Percentage of aboundance and diversity of phytoplankton changed in 2002 inrelation to years ago( before shrimp culture, 2000 year and 2001 year) specially in Gwatar Gulf. Mainly percentage of zooplankton were copepoda and thintinida respectively.Diversity zooplankton decreased ratio to years ago. In drainage channel, abundance total of phytoplankton and zooplankton were more than Gwatar Gulf and supply channel.
    Keywords: Aquaculture ; Iran ; Sistan and Blouchestan ; Gwatar ; Chabahar ; Environmental effect ; Shrimp culture ; Survey ; Sampling ; Temperature ; Salinity ; Dissolved oxygen ; pH ; Chlorophyll a ; Phytoplankton ; Zooplankton ; Diatoma ; Cyanophyta ; Chlorophyta ; Copepoda
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  • 4
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    The monitoring of hydrology and hydrobiology stydies of the Persian Gulf in the Bushehr region (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25333 | 18721 | 2018-09-12 09:59:44 | 25333 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This study has been carried out in waters of Bushehr province in the Persian Gulf covering Nayband bay to Bahregansar between 26° 45 N to 30° 00 N and 49° 45 E to 52° 45 E, 15 main and 5 sub- stations, by R/V Ferdows 1 in winter 2004, summer 2004 and winter 2005. The physico - chemical parameters, nutrients (silicate, phosphate, nitrate and nitrite), phyto, zooplanktons, sediments and macrobenthoses were collected from water layers and sea bottom, and then analysed. The parameters were compared and evaluated with the results of the previous work, conducted in the same area. Termocline, halocline, oxycline and picnocline layers were formed in summer. These clins disappeared due to water column mixing in winter. The values of temperature, conductivity and pH in summer were higher than those of in winter, while the salinity and density values of surface and subsurface layers in winter were higher than those of in summer. The temperature and conductivity have been decreased from surface to depth, from coastal rigions to offshore and from southern waters to northwern waters, while salinity, density and turbidity have been increased. The dissolved oxygen is decreased by increasing the values of temperature, salinity and depth and it is observed that D.O. has increased in correlation with chlorophyl a in mean and subsurface layers. The pH was rather decreased from surface to depth while turbidity was increased. Orthosilicate (Si_SiO44), nitrate (N_NO3), and nitrite (N_NO2 ) concentration have increased from surface to depth while orthophostate (P_PO43) decreased. Among the phytoplanktons, Dinophycea had most abundance in winter 2004 and Diatoms had most abundance, variety and distribution in summer 2004 and winter 2005. Among the zooplanktons, Copeopda had most abundance, variety and distribution. The intensity of phytoplanktons in summer was more than those in winter; it decreased from surface to depth and from coastal rigions to offshore, at first increased and then gradually decreases. Intensity of zooplanktons in summer was more than those in winter and decreased from surface layers to depth and from coastal waters to offshore. The abundance of benthoses in summer was more than that in winter while their biomass in winter was more than that in summer. The abundance decreased from southern to northwern waters, but the rate of decrease in biomass was more than that of abundance. The mainly texture of sediments was loamy or muddy and the grain size in southeastern and northwestern areas were coarser than those in middle area. Compared with the previous study, the trend of physico chemical, silicate, nitrate and nitrite were the same but in spite of the past, the values of pH in summer were more than those in winter and also phosphate concentration had been decreased frome surface layer to depth. The overall trends of phyto and zooplanktons densities are almost similar to previous observation, but cyanophyceae significantly decreased, in comparison with spring and summer abundance. It is also observed that the zooplanktons average density has been in reverse order compared to previous observations. Average concentration of phosphate in the entire region was seven times more than that of the former study and its maximum value has rised more than 26 times in the petroleum and gas industries areas. On the other hand, the abundance and diversity of phyto and zooplanktons and also the ratio of biomass to abundance of benthoses were decreased.
    Keywords: Ecology ; Iran ; Persian Gulf ; Bushehr Region ; Nayband ; Bahregansar ; Hydrology ; Hydrobiology ; Nutrients ; Phytoplankton ; Zooplankton ; Sediments ; Macrobenthose ; Temperature ; Dissolved oxygen ; Chlorophyll a ; pH ; Diatoms ; Copeopda ; Depth ; Monitoring
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  • 5
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    Comparative survey of native species culture (Penaeus semisulcatus and Fenneropenaeus indicus) with non-native species (Litopenaeus vannamei) in Hele site of Bushehr province (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25339 | 18721 | 2018-09-14 06:59:46 | 25339 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The culture of three species green tiger prown (Penaeus semisulcatus) ,white leg shrimp (Litopenaeus vannamei) and Indian white shrimp (Fenneropenaeus indicus) were grown in 7 ponds (0.4 and 0.25 hectare) for 110 days at a stocking density of 25 shrimp/m2 on Hele Research Station. The average of temperature and salinity was 30 degrees centigrade and 46-48 ppt .At the end of the culture mean weights of L.vannamei 18.68±2.6 was higer than the P.semisucatus 7.77±1.49 and F.indicus 14.66 ±2.1 also results demonstrated that there was a significant relation (P〈0.05) between mean weight. Final yield were 3114, 1983 and 848 Kg for L.vannamei, F.indicus and P.semisucatus.The FCR was better for L.vannamei (1.28) than F.indicus (1.48) and P.semisucatus (2.15).
    Keywords: Aquaculture ; Iran ; L.vanname ; F.indicus ; P.semisucatus ; Mean Weight ; Culture ; Native species ; Green tiger prown ; Penaeus semisulcatus ; Shrimp ; Indian white shrimp ; Temperature ; Salinity ; FCR
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  • 6
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    Feasibility study on pomferet (Pampus argentus) breeding technique in Imam marine research station (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25342 | 18721 | 2018-09-13 12:35:36 | 25342 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This study was conducted during April to June 2007 .Pomferet brooders were captured by gill net (5 m Height, 300 m length, 7 cm size) during tidal time with 20-40 minutes interval. Khor Mermis, Khor Patil and Khor Mavi were subjected for broodstock captures. Totally 237 brooders were captured (126 male and 111 female) and were kept in 300 L. tanks. Semi dried method were used for breeding with 0.6 % fertility solution. Hatching were placed during 16-18 hoers. Fish larvae were kept in 4 T. tanks and were fed by fresh rotifer .temperature and salinity was kept 31-28 degree centigrade and 45-47 ppt. respectively .results shows successful breeding of pomferet in 2007 .egg diameter was 1.1 mm and hatching were completed during 16-18 hours at 31-29 degree centigrade. After 31 days length of larvae was 40 mm. while for 7 and 15 days length of larvae was 4.5 and 8.4 mm respectively.
    Keywords: Aquaculture ; Iran ; Feasibility ; Pomferet ; Pampus argentus ; Breeding ; Marine ; Gill net ; Height ; Length ; Size ; Broodstock ; Fertility ; Larvae ; Temperature ; Salinity
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  • 7
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    Effect of synthetic and algal astaxanthin on the reproduction efficiency and growth of larval stage in rainbow trout (Oncorhynchus mykiss) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25349 | 18721 | 2018-09-14 07:19:02 | 25349 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This research was done in a trout farm in Kohkiloyeh and Boyer- Ahmad province. The main object of this study was comparing of two sources of astaxanthin (synthetic or algae) in feed on reproduction efficiency of rainbow trout and some indices of egg quality. Therefore, it was considered 7 groups consisting 6 treatments of two different astaxanthin sources and a control (without astaxanthin). So, algal astaxanthin (haematococcus pluvialis) in the three levels of 2.67, 3.55 and 8gr/kg food (T1,T2, T3) and synthetic source in three levels of 40, 80 and 120mg/kg food in diet (T4, T5, T6) examined on 140 trout broods (3-4 years) for 4 months, before the spawning season. Reproduction efficiency analyzed through reproduction indices such as egg diameter, fecundity, fertilization, survival, hatching and eyed egg rates. Significant difference between treatments in terms of fecundity, eyed eggs rate and the number of eggs per gram was observed (P 〈0.05). Although in some factors such as diameter, egg weight per body and fertilization rate, no significant differences was observed. By increasing level of astaxanthin in both sources of algal and synthetic, hatching and eyed egg rates increased, but the effect of alga source on these indices was more perfect. Considering the results, the best result related to treatment of 8 gr/kg alga (T3). Observations during this research indicated no disease and mortality and also proper coloration and freshness of fishes. There was significant differences on blood factors (P 〈0.05). The broods were fed with algal astaxanthin had lower blood sugar. concentration of cholesterol was decressed alog with increasing in both the source of astaxanthin, however lower cholesterol relaed to algal astaxanthin. Total serum protein increased in treatments containing algal from 2.67 to 8 g/kg of astaxanthin. Increase in level of astaxanthin from 40 to 80 mg/kg, albumin increased and from 80 to 120 mg/kg, it was decreased. The changes in serum globulin treatments containing synthetic was not regular. Overall the results asserted that the application of astaxanthin improves reproduction performance of rainbow trout. It also concluded that natural astaxanthin (Haematococcus pluvialis) for the reason that contains supplementary nutritious, is extraordinary preferable than synthetic astaxanthin to improve reproductions indices and egg quality of rainbow trout.
    Keywords: Aquaculture ; Iran ; Kohkiloyeh and Boyer- Ahmad province ; Rainbow trout ; Astaxanthin ; Haematococcus pluvialis ; Reproduction efficiency ; Larval stage ; Broods health ; Synthetic ; Algal astaxanthin ; Growth ; Oncorhynchus mykiss ; Eggs ; Spawning ; Fecundity ; Fertilization
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  • 8
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    Feasibility study on semiintensive polyculture of chainess and Indian carps in I.R.IRAN (Guilan province) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25352 | 18721 | 2018-09-14 06:57:48 | 25352 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: 15000 juvenile Indian majour carps (Labeo Rohita,Cirhinus merigala, Catla catla) imported (200 ) and transferred to the earthen ponds in Astaneh Fisheries Research Station , In order to assess the viability of rearing under the climatic condition of north part of Iran and also achieve some growth parameters from the larval stage to market size.The fry with 300 mg weight, were released in to 12 ponds. Specimens were separated to tree experimental categories: 1= (30%indian carp and 70% chinees carp) , 2= (50%indian carp and 50% chinees carp) , 3= (70%indian carp and 30% chinees carp) .single chinees carp production were used as control . fishes were fed with artificial food and also with the natural products of the ponds which were enriched with organic fertilizers, after being equally stocked in the ponds .During the experiment, oxygen level ,PH, turbidity, and temperature were measured. Monthly fry samples were taken, for weight and length biometrical analysis and also determination of the weight gain percentage, average daily growth rate, specific growth rate. Results have indicated adaptations and considerable growth in this species, with an increase from the initial weight of 300 mg to an average of 13.5±1.6 mg in 50 days period until the end of November for fingerlings. Also the average weight of growing phase (second year of production ) was 647g for Labeo Rohita ,420 g. for Cirhinus merigala and 734g for Catla catla. The special growth rate (SGR)in Roho was 4.51 %.and 3.9 % for merigal and6.2 % for Catla while for Chinees carps SGR was 4.9, 6.7, 7.6 and 5.8 for silver carp,grass carp, common carp and big head respectively. Results revealed that the Indian majour carps compatibility with the condition in Gilan province was succesfull and the combination of species and optimization of their compatibility were remind for futhre studies.
    Keywords: Aquaculture ; Iran ; Guilan Province ; Astaneh ; Indian carp ; Growth ; Labeo Rohita ; Cirhinus merigala ; Catla catla ; Polyculture ; Juvenile ; Earthen ponds ; Fisheries ; Rearing ; Larval stage ; Fry ; Specimens ; Artificial food ; Fertilizers ; pH ; Turbidity ; Temperature
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  • 9
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    Possibility study on using an Iranian cottonseed meal variety instead of soybean meal in the rainbow trout (Oncorhynchus mykiss) diet (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25365 | 18721 | 2018-09-14 07:19:43 | 25365 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This study was set up to investigate the nutritional value and the optimum inclusion level of one Iranian cottonseed meal i.e. var. Pak, (CSMP) as a substitute for soybean meal (SBM) in rainbow trout (Oncorhynchus mykiss) feed. Apparent digestibility coefficients (ADC) for these varieties were calculated in experiment 1 by using an indigestible marker (chromic oxide). Six formulated feeds consisting different substitution levels (0, 20, 40, 60, 80, 100%) of SBM with CSMP, CSMS, and CSMA, respectively, were fed to a total of 540 rainbow trout with initial mean body weight of 50 ± 5 g. Fish were randomly stocked into eighteen 100 L. fiberglass tanks with 30 fish per tank and 3 tanks per diet and fed to satiation 3 times a day for 8 weeks. At the end of each separate experiment, 3 fish from each replicate for each dietary treatment were sacrificed to measure the whole fish body proximate compositions and liver gossypol content. After an 8 week feeding trail, the average weight gain, FCR, SGR, PER, DWG, PWG and CF of fish fed with different diets were measured separately. For all treatments, the survival percentage was more than 98%. Results showed that 100% and 80% CSMP showed in better growth in each experiment and 100% CSMP wasthe best in weight gain (104.3 g), FCR (1.5) and SGR (1.25%).
    Keywords: Aquaculture ; Iran ; Cotton seed meal ; Gossypol ; Nutrition ; Rainbow trout ; Soybean meal ; Oncorhynchus mykiss ; Diet ; Survival ; Growth ; Weight gain
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  • 10
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    Assessment of different methods of water treatment on decreasing pathogenic bacteria in shrimp hatcheries (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25373 | 18721 | 2018-09-14 07:27:49 | 25373 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The project of "Assessment of different methods of water treatment on decreasing pathogenic bacteria in shrimp hatcheries" achieved by mean of reviewing the effectiveness of various methods on the water treatment to reduce bacterial load of sea water. The project performed in Bandargah region of Bushehr province in order to checking the effect of different methods on the sea water and bacterial population. This project has been done over a year. In this study a total of 138 samples was collected during seven times and was evaluated as well as 7 treatments. In this study, the comparison of the effect of each factors (sand filter, chlorine, ultra violet ray, chlorine combined with ultra violet ray) was analyzed on the sea water by means of disinfection. During this study, other environmental factors such as water temperature, dissolved oxygen, pH and salinity was measured till we can ensure that they don’t have any negative impact on the treatments. Results showed that the density of total bacteria in sea water (Control) was averagely 5187 CFU/ml, this was done while the total density of bacteria in the sea water, reservation pool sample, sand filter water, water that irradiated with ultraviolet rays, chlorinated water and the water that was influenced by both chlorine and ultra violet ray was respectively 5187 2137, 4449 2042, 2782 1335, 1164 692, 143 104, 87 76 CFU/ml. Therefore, reservation pool, sand filter, ultra violet ray, chlorine, chlorine-ultra violet ray each of them was reduce respectively 14, 46, 77, 97, 98 percent of the density of total bacteria or in other words they reduced 0.06, 0.27, 0.65, 1.56, 1.77 log of the total population of bacteria in water. Moreover, the results from the total count of vibrios showed that reservation pool, sand filter, ultra violet ray, chlorine, chlorine-ultra violet ray each of them was reduce respectively 17, 47, 74, 98, 99 percent of the density of total vibrios or in other words they reduced respectively 0.08, 0.27, 0.59, 1.66, and 1.95 log of the total population of vibrios in sea water. Also the bacteria that isolated from each treatment were identified with the use of biochemical methods that totally were includes a wide range of bacteria, the identified vibrios were: Vibrio alginolyticus, V. costicola, V. fischeri, V. fluvialis I, V. fluvialis II, V. harveyi, V. natriegens, V. nigripulchritudo, V. parahaemolyticus, V. plagius I, V. vulnificus, V. anguilarum I, V. campbellii, V. nereis, V. proteolyticus, V. splendidus, and Unknown V. spp. Other identified bacteria belonging to the following genus: Acinetobacter, Aeromonas, Alcaligenes, Bacillus, Borkholderia, Eschricia, Enteroacter, Flavobacterium, Micococcus, Proteus, Pseudomonas, Serratia, Staphaphylococcus, Streptococcus, and Unknown Bacteria spp. By comparing the results, we concluded that the best result was related to treatment that the sea water had been disinfected after the combined effects of chlorine and ultraviolet ray. It was determined that this process in compared with other treatments, had been reduced the total number of bacteria, the total number of Vibrios, the number of sucrose fermentative vibrios, and the number of non-sucrose fermentative vibrios respectively 98, 99, 98, 99 percent. In other words, the number of bacteria was reduced respectively 1.77, 1.94, 1.82, 2.23 log. So in this study, the applied method was introduced as the most effective way of disinfecting sea water. Meanwhile, none of pathogenic bacteria for shrimp including Vibrio harveyi was isolated by using this treatment. Comparing the results showed that the combined effects of chlorine and ultraviolet radiation is the most effective applied method for disinfecting sea water.
    Keywords: Aquaculture ; Iran ; Bushehr province ; Bandargah region ; Shrimp ; Water treatment ; Sea Dissolved oxygenwater ; Bacteria ; Hatchery ; Assessment ; Population ; Temperature
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    The study on possibility of fish soup powder production from different fish species in Sistan O blochestan Province, Chabahar (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25361 | 18721 | 2018-09-14 07:10:40 | 25361 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Fish soup powder is produced from three kinds of fish in Chabahar catched in Oman sea white flesh of many low value fish like threadfin bream, perches etc. can be used to prepare instant fish soup powder. This product has a high consumer acceptability in many countries in this research project we used 4 low value fishes with local name of Sarm, Chaman, Aroos and Talal belonging to families Carangidea, Lethrinidea, Drepanidae and Scombroidea. soup powder packed in laminated pouches and the shelf life of it is one year in ambient temperatures. The quality used to be good up to 6 month but after this time the quality decreases gradually. The experiment on fish soup showed that the percentage of protein was 32 percent, fat 11 percent and humidity 12 percent and ash 4 percent. The experiment conducted on fish soup powder was peroxide, total microbial count, organoleptic test for 6 month.
    Keywords: Fisheries ; Iran ; Oman sea ; Sistanblochestan province ; Chabahar ; Fish Soup Powder ; Species ; Fish ; Threadfin bream ; Carangidea ; Lethrinidea ; Drepanidae ; Scombroidea ; Temperature ; Protein ; Microbial count
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  • 12
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    Introduction of serum proteins and immunoglobulin's in Oncorhynchus mykiss and Ctenopharyngodon idella (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25377 | 18721 | 2018-09-14 07:42:27 | 25377 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The concentration of serum immunoglobulin in rainbow trout (Oncorhynchus mykiss) and(Ctenopharyngodon idella)was measured by Immunoelectrophoresis. Serum total protein, also called plasma total protein or total protein, is a biochemical test for measuring the total amount of protein in blood plasma or serum. Protein in the plasma is made up of albumin and globulin. The globulin in turn is made up of α1, α2, β, and γ globulins. These fractions can be quantities using protein electrophoresis, but the total protein test is a faster and cheaper test that estimates the total of all fractions together. The traditional method for measuring total protein uses the biuret reagent, but other chemical methods such as Kjeldahl method, dye-binding and Refractometer are now available. The measurement is usually performed on automated analyzer salong with other laboratory tests. The normal IgM concentration was 3.3 mg ml in a group of free-living trout. While the IgM concentration was low in sera from fish living under aquarium conations. In visual variations were very pronounced. The purity of reference preparations and the specificity of anti sera used were examined by crossed Immunoelectrophoresis. Fish respond to antigenic stimulation by the production of immunoglobulin. So far only one immunoglobulin class is known to occur in teleosts, the characteristics of the class being rather similar to those of mammalian IgM. The molecule is a tetramers consisting of a basic structure of 8 light chains and 8 heavy chains (same molecular weight as the p-chains of mammals) (Acton et al. 1971, Etuis 1982). The molecular weight of the whole molecule is about 700 000 Daltons (13 to 16 S). Most studies concerning the humoral immune response in teleosts have dealt with aracteristics of the immune response elicited by known antigens. Only in a few cases has the concentration of total immunoglobulin been measured. Estimates of total immunoglobulin have been made in serum from (Oncorhynchus mykiss) and (Ctenopharyngodon idella) carp and goldfish (Vilain et al. 1984), carp (Richter et al. 1973), brown trout (Ingram & Alexander 1979) and certain salt-water fish (Fidler et al. 1969, Acton et al. 1971, Legler et al. 1971). Estimates of total IgM in serum from rainbow trout Salmo gairdnen have not, to our knowledge, been published so far.Serum. Blood samples were obtained by puncture of the caudal vein of rainbow trout (Oncorhynchus mykiss) and(Ctenopharyngodon idella)After clotting overnight at 4 "C the blood was centrifuged at 1000 g for 15 min to obtain serum. The sera were stored at -20 "C until examined. Total serum protein. Protein concentration in (Oncorhynchus mykiss) and(Ctenopharyngodon idella) sera was estimated by means of the Biuret method(Richtenich 1971). Antiserum to IgM : The monospecificity of the rabbit antiserum to rainbow trout IgM was indicated by the appearance of only one precipitation Line when the antiserum was reacted against normal trout serum in crossed Immunoelectrophoresis (Fig. 1). The purity of the IgM preparation which was used as reference IgM in connection with IgM quantification is illustrated in Fig. 2a. It appears that only one precipitation line developed when the preparation was reacted against antiserum to trout serum in crossed Immunoelectrophoresis. Fig. 2b illustrates the multi specificity of the rabbit antiserum to trout serum used.
    Keywords: Health ; Iran ; Oncorhynchus mykiss ; Ctenopharyngodon idella ; Immunoglobulin ; Serum total protein ; Rainbow trout ; Plasma ; Blood ; Globulin ; Carp ; Goldfish
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  • 13
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    Investigation of production possibility of monosex female and sterile fish in rainbow trout Oncorhynchus mykiss (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25147 | 18721 | 2018-08-26 16:54:41 | 25147 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This investigation carried out for the first time in Iran inorder to prodcution of monosex female and also sterilization in Rainbow trout. In this study, the eggs of general females were fertilized with the sperm of sex reversed male and so monosex female population was produced in second generation and sterilization carried out with oral administration of 17α methy 1 testosterone and immenrsion and oral administiration methods were used in embryonic stage and from commencing of acitve feeding of larvae, respectiverly. For sex reversal , 13 treatments were considered totally, that the most percentage of male (100%) was observedc in a treatment including of orally administration of 0.5 ppm hormone for 60 days after commencing active feeding (P〈0.001). In the other treamtnet, different percentages of sex ratio including male, female, intersex and sterility were observed. The offspring of genral eggs fertilization with the sperm of masculinized fish were 100% female, chisquare test was shown the treatment of orally administration of 30 ppm hormone for 120 days after commencing active feeding that had been considered for sterilization, was produced 90% sterile fish (P〈0.001) and was changed the sex ratio significancthy. Morphological changes of the gonads and sperm ducts in matured fish and also histological changes in the gonads of fish in the treamtints were considerable.
    Keywords: Biology ; Iran ; Monosex ; Female ; Male ; Sterilization ; Rainbow trout ; Eggs ; Fertilized ; Sperm ; Population ; Sex ; Fish ; Oncorhynchus mykiss ; Rainbow trout
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  • 14
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    Monitoring of Cochlodinium sp for shrimp farms in Hormozgan Province (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25423 | 18721 | 2018-09-23 16:29:10 | 25423 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The Blooming due to the some species of phytoplanktons especially Dynoflagellates has made some problems for water ecosystems and aquaculture. In this study, the density of phytoplanktons specially Cochlodinium sp. and also environmental factors such as temperature, pH, dissolved Oxygen, and transparency were recorded two weekly in 18 stations of Hormozgan province, Iran in order to monitoring of the possibility of phytoplankton blooming. During six months monitoring, the target phytoplankton, Cochlodinium sp was not observed in shrimp farms. But, other phytoplanktons and zooplanktons were observed as follow: 13 genus of phytoplankton and six genuses of zooplanktons has found in ponds, main water channel and sea. The diatoms with 10 genuses had the highest abundance and Dynoflagellates with 3 genuses had the lowest abundance and blue-green phytoplankton with one genus was in lowest group. Totally, diatoms with 77%, Dynoflagellate with 15% and blue-green alga with 8% abundance were the main populations of planktons in the studied area.
    Keywords: Aquaculture ; Iran ; Hormozgan Province ; Tiab ; Algae Cochlodinium sp ; Shrimp farms ; Species ; Phytoplanktons ; Dynoflagellates ; Aquaculture ; Temperature ; pH ; Dissolved oxygen ; Transparency ; Zooplanktons ; Blue-green algae
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    Determination of effective parameters on growth and bloom forming of Cochlodinium polykrikoides (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25436 | 18721 | 2018-09-23 16:56:01 | 25436 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Harmful algal blooms resulting in red discoloration of coastal waters in the Persian Gulf, Iran were first observed in January 2007. The species responsible for the bloom, which was identified as Cochlodinium polykrikoides, coincided with massive aquatic organisms’ mortalities in the Persian Gulf. In order to provide optimum growth and bloom forming, C. polykrikoides cells were sampled during the bloom conditions in the coastal waters of Persian Gulf. After adaptation in filtered seawater, they isolated by positive phototropism characteristic of this species to light. They were grown in modified media culture at different salinity (30, 32 and 35ppt), temperature (20, 23, 26 and 28ºC) and intensity (35, 70 and 90 µmol m^-2 s^-1) with an initial cell density of 50 cell mL^−1. The results of the present study clearly showed that the highest alga biomass was obtained following culture by using A^2 medium under the 32ppt salinity, 26°C temperature, and under a 11h light:13h dark photoperiod regime at a light intensity of 90 µmol m^-2 s^-1 provided by cool white fluorescent tubes. Mean cell density of C. polykrikoides in a 60 liter tank for ten days reached to 32 × 10^6 cell L^−1. Moreover, individual C. polykrikoides chain with 18 cells was observed for the first time in cultures. Based on the results from the present study, as mentioned above, providing suitable media culture and physical condition (light intensity and temperature), bloom forming of C. polykrikoides start from day 8 to 10 and will be continued until day 24 t0 28. In the other hand, C. polykrikoide cells immediately crashed and destroyed.
    Keywords: Ecology ; Iran ; Persian Gulf ; Harmful algal bloom ; Cochlodinium polykrikoides ; Isolation ; Optimum growth ; Environmental parameters ; Phototropism characteristic ; Species ; Density ; C. polykrikoides ; Temperature ; Growth ; Biomass ; Bloom
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    Study on viral nervouse necrosis (isolation, characterisation and pathogenesis) in golden grey mullet in the Caspian Sea and study of pathogenecity and possibility of transmission to the other fish species (Sturgeon fishes, Rutilus frisii kutum and reared Rainbow trout and Carp) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25435 | 18721 | 2018-09-23 16:47:09 | 25435 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Study on Viral Nervouse necrosis (isolation, characterisation and pathogenesis) in Golden grey mullet in the Caspian Sea and study of pathogenecity and possibility of transmission to the other fish species (Sturgeon fishes, Rutilus frisii kutum and reared Rainbow trout and Carp) Viral Nervous Necrosis (VNN) is a worldwide disease affecting several species of cultured marine fish. For the past two decades, betanodavirus infections that cause Viral Nervous Necrosis (VNN) have emerged as major constraints on the culture and sea ranching of marine fish in almost all parts of the world. More than forty species mainly of marine origin have been so far affected and this number is likely to rise in future following the introduction of new species and the increase of aquaculture trade. Unknown acute mortality occurred in wild golden grey mullet Lisa auratus and Liza saliens in Iranian waters of Caspian Sea in recent years. In order to isolation and confirmation of causative agents of golden grey mullet mortality in the Caspian Sea, a complementary research investigation project was designed in 2005 and approved immediately in Iranian Fisheries Research Organization (IFRO). Many diagnostic aspects such as Virology (Cell culture and Elctereone Microscopy), Hemathology, Bacteriology, Histopathology, Molecular biology (NestedRT-PCR), Heavy metals measuerment and Serology (IFAT and IHC) were employed in mentioned multidiciplinary project. About 322 moribund fish samples which revealed skin darkening, erratic swimming behavior such as spiral and belly-up at rest and high distention of swimming bladder. Suspected samples were collected from coastal capture sites in iranian north proviences in 2006 till 2009. Targets tissue such as brain and eye were removed in strile condition and then kept in -80oC frezzer for cell culture and Nested-RT-PCR. Other tissue samples from liver, kidney, intestine, stomach, gill, skin and muscle, gall bladder and gonads were taken and fixed in 10% buffer formalin and same parts fixed in glutaraldehyde 3% for histopathology, IHC and EM respectively. Cytopathic effect (CPE) was observed in those cell cultures just six days after inoculation with the dilutions of the tested 312 homogenate supernatants. CPE in monolayers of cells cultured (SSN-1 cell line) was characterised by thin or rounded, refractile, granular cells with vacuoles. Nine samples were positive in virology assay. Nested- RT-PCR was done on suspected tissue samples and supernatant of CPE positive samples and 21 tissue samples and all CPE positive samples were positive. IFAT was selected as a confirmatory method for identifying viral strains replicating on cell cultures and carried out with rabbit anti-betanodavirus serum on suspected tissue samples and some smears of CPE positive samples. Some bright points approved betanodavirus antigen and confirmed cell culture and Nested-RT-PCR findings. In fixed tissue samples widespread and massive vacuolation were observed in brain, spinal cord, retina and optical nerve. In order to confirmation of diagnostic findings , IHC was done with monoclonal antibody antibetanodavirus and some red-brown points were observed. Theses findings revealed expected viral antigens and confirmed previous results. Moreover, virus particles with 25-30 nm in diameter were visualized in infected brain and retina using positive staining in TEM. Also pathogenicity test was employed to confirm the obtained results. So Guppy fish Poecilia reticulata and sturgeon fry were used instead of the experimental host due to ease of handling and susceptibility. After 15 days post infection, guppy bathed in VNN-infected tissue culture with 104 TCID50 showed clinical signs similar to naturally infected Golden grey mullet, and the mortality rate reached up to 100% in 75 dpi. When target organs were examined by cell culture isolation, serology, and histopathology, all revealed the presence of virus in the Guppy. Suspected supernatant injected to sturgeon fry through intravitreous injection and widespread vacuolation were observed in brain and spinal cord buy IHC and Real time PCR were negative. In conclusion, with attntion to obtained results in this investigation such as ecological factors, clinical signs, histopathological, virological and bacteriological results, molecular analysis, (IHC, IFAT, PCR), TEM demonstration, serological and hematological findings, it could be confirmed that VNNV was the main causative agent for disease outbreak in Golden grey mullet in Southern coastline of Caspian Sea.
    Keywords: Aquaculture ; Health ; Iran ; Caspian Sea ; Viral nervous necrosis ; Golden grey mullet ; Liza aurata ; Liza saliens ; Histopathology ; Virology ; Bacteriology ; IHC ; IFAT ; PCR ; TEM ; Species ; Sturgeon ; Rutilus frisii kutum ; Rainbow trout ; Carp
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  • 17
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    Effect of Echinacea purpurea and Astragalus glycyphyllos on immune responses of rainbow trout (O. mykiss) and resistance to sterptococcosis (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25451 | 18721 | 2018-09-23 17:53:51 | 25451 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Nowadays, medical plants extract is used in aquaculture industry to enhance growth, immune system and fish resistance to different pathogens. In this study, some non-specific immune responses, hematology and biochemical parameters in rainbow trout juveniles (16 gr mean weight) and their resistance to Streptococcus iniae were evaluated following oral administration of Echinacea purpurea and Astragalus sp. extract. Three concentrations (0.5, 1 and 1.5 gr/kg of feed) of Echinacea purpurea and three concentrations (2, 3 and 5 gr/kg of feed) of Astragalus sp extract and control group were used for 60 days. Parameters evaluated included: levels of C4, C3 (complement components), free oxygen radicals (respiratory burst), Lysozyme, numbers of lymphocytes, monocytes and neutrophils. In the end, the relative survival rate (RSR) of fish was evaluated against S. iniae. The results showed that the levels of C3, lysozyme, oxygen free radicals, the number of neutrophils and lymphocytes in the experiment groups (the highest dose) compared to control group were increased significantly (p 〈0/05). While, the values of C4 and number of monocytes compared to the control group were not significantly different (p〉 0.05). The relative survival rates of fish following challenge with Streptococcus iniae, were 91/11, 93/33 and 44/44 percent in experiments (Echinacea purpurea and Astragalus sp.) and control groups, respectively. In conclusion, it may be state that Echinacea purpurea and Astragalus sp. extracts enhance the non-specific immune system and fish resistance against Streptococcusis, and it seems it can be used as immunostimulant in feed.
    Keywords: Aquaculture ; Iran ; Echinacea purpurea ; Astragalus sp ; Rainbow trout ; Streptococcusis ; Immune system ; Astragalus glycyphyllos ; O.mykiss ; Aquaculture ; Growth ; Juvenile ; Lysozyme ; Survival rate
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    The use of modified atmosphere packaging for improvement shelf life of fresh Oncorhynchus mykiss (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25168 | 18721 | 2018-08-26 17:35:37 | 25168 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: In this study, influence of modified atmosphere packaging on shelf life of trout (Oncorhynchus mykiss) (whole fish without visceral and without head and tail fish) stored in 4 to 6ºc was examined. Fish stored in MAP condition and control samples, in different time, were tested for spoilage chemical factors (TVN, PV and pH), microbial parameters (total viable count, clostridium botulinum) and sensory factors too. Mixed gases including co2 (30-50%), N2 (40-65%) and o2 (0 to 20%) were used for trout (without head and tail =6 treatments) and (whole fish without visceral and control = 2 treatments) statistical the analysis results showed that examined factors were significant difference during storage (P〈0.001). Mixed gases haven t had inhibitory effect on spoilage factors (chemical and microbial parameters). However spoilage process was delayed. Increasing of chemical and microbial changes in control samples was higher than treatment samples especially TVN. The results also showed that shelf life of control samples stored 4-6ºc were between 6-12 days but in MAP samples were 19 days. Mixed gases including CO2 (40%), N2 (55%) and O2 (5%) were the best formula and the shelf life of fish (without head and tail) was 16 days where it was 19 days in whole fish (Lack of visceral). The results showed that storage of trout in MAP condition facilities storage and increasing of fish shelf life too.
    Keywords: Fisheries ; Iran ; Oncorhynchus mykiss ; Modified Atmosphere Packaging ; Shelflife ; Rainbow trout ; Samples ; pH ; Microbial parameters ; CO2 ; N2 ; O2
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  • 19
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    Study on effects of Beta-glucan & extraction of Sargassum & Padina algae in prevention and treatment of WSD in Fenneropenaeus indicus (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25179 | 18721 | 2018-09-03 17:23:17 | 25179 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: In this study two species of algae, Sargassum glaucescens and Padina boergeseni that found plenteously in Persian Gulf and Bushehr coast, were collected and hot water extracts of them were lyophilized. F. indicus (11.32±1.20 g), after two weeks adaptation in Shoghab research station were immersed in seawater (39 ppt and 25±1 ○C) containing hot-water extract of each brown algae, S.glaucescens and P.boergeseni, at 100, 300 and 500 mg/l concentration, Survival rate and immunological parameters (total haemocyte count (THC), total plasma protein (TPP), Phagocytic activity, bacterial clearance efficiency and bactericidal activity) were examined. In addition effect of dietary administration of beta 1, 3 1, 6 glucan on prevention of White Spot Disease and immunological parameters of shrimp were investigated. According to results, immersion in seawater containing 300 and 500 mg/l concentration of algal hot-water extract after 2 and 3 hours or oral administration of beta 1,3 1,6 glucan at level of 10 g/kg diet for 14 days significantly enhanced THC, TPP, Phagocytic activity, bacterial clearance efficiency and bactericidal activity. Immersion in seawater containing 100, 300 and 500 mg/l hot-water extract of S.glaucescens after 3 hours, improved the survival rate of WSSV-infected F. indicus.
    Keywords: Biology ; Chemistry ; Iran ; Persian Gulf ; Bushehr Province ; Beta-glucan ; Sargassum ; Padina algae ; WSD ; Fenneropenaeus indicus ; Species ; Algae ; Brown Algae ; S.glaucescens ; P.boergeseni ; Survival rate ; White Spot Disease ; Shrimp
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    Preliminary cultivation of silver bream (Sparidentexhasta) in earthen pond using available artificial fish feed in Chowibdeh site-Abadan southern Iran (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25183 | 18721 | 2018-09-03 17:12:18 | 25183 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Possibility of rearing silver bream (Sparidentex hasta) in the earthen pond was experimented using 2 types of available artificial trout feed (FFT and GFT1). The experiment was carried out in 2 phases in Shahid-Kiani Extension Shrimp Farm in Chowibdeh Shrimp Site-Abadan Southern Iran, and earthen ponds with 0.25 ha. Area were used for both phases of the study. Juvenile fish of 3.2 g initial weight with the density of 9324 ind./ha. Were stocked for 196 days in the first phase. The initial weight, stocking density and rearing period of the fish in the second phase were 108.60 g, 9800 ind./ha. And 237 days respectively. Maximum amount of WG, FCR, PER and SVR of the fish in the first phase of the study were 100.12 g, more than 6, 0.38 and 55.32% respectively. WG and SR of the fish in the second phase respectively were 417 g and 20.74%. The Salinity of pond water was in the range between 9 and 22 ppt, and the water temperature was between 8.3 ֯C (mid December morning) and 34.8 ֯C (early Sep.....
    Keywords: Aquaculture ; Iran ; Abadan ; Chowibdeh ; Cultuation ; Silver Bream ; Sparidentexhasta ; Earthen Pond ; Artificial feed ; Rearing ; Shrimp ; Juvenile ; Weight ; Density ; Temperature
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  • 21
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    Biological survey on small pelagic fishes (sardine & anchovy) stocks in coastal waters of Hormuzgan province (Qeshm Island & Bandar Lengeh) with emphasis on sea surface temperature, plankton distribution and chlorophyll-a (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25198 | 18721 | 2018-09-05 16:25:43 | 25198 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Investigation on small pelagic fishes in the north of the Persian Gulf (Qeshm Island and Bandar Lengeh) coastal waters during October 2006 to September 2008, illustrated that 6 sardine and 2 anchovy species were locally existed. Sind sardinella (Sardinella sindensis) and Buccaneer anchovy (Encrasicholina punctifer) were dominated in this area. The peak of sexual maturity for Sind sardinella and Buccaneer anchovy occurred in spring and summer respectively. Reproduction period for Sind sardinella was from the middle of winter to late spring, and Buccaneer anchovy encompassed reproduction capacity all the year. The female: male ratio was calculated 1:0.7 and 1:0.9 for Sind sardinella, 1:1.27 and 1:1 for Buccaneer anchovy in Qeshm Island and Bandar lengeh sequentially. The mean of absolute fecundity were estimated 16234 and 16168 for Sind sardinella, 1277 and 1141 for Buccaneer anchovy in Qeshm Island and Bandar Lengeh respectively. L_M50 and L_C50 were calculated and the results showed both species mainly caught before length of maturity, and the mass populations have not sufficient opportunity to reproduction. Both species were planktonivore, theirs food prefect mainly consisted of copepods, naplius and bivalves. Vacuity index indicated both spices were semi-voracious appetite fishes. Based on calculated length-weight relationships in S. sindensis and E. punctifer, they have isometric growth. Growth parameters were estimated, the results illustrated that they were growth fast and short live species, which suggested S. sardinella and E. punctifer have 3 and 2 years maximum life span respectively. The recruitment pattern indicated double recruitment peaks per year in both species. Annually, four cohorts were distinguished in Sind sardinella and 3 cohorts in Buccaneer anchovy. Total mortality (Z), natural mortality (M) and fishing mortality (F) were estimated in both species, and the values of exploitation ratio (0.78 & 0.74) in Sind sardinella and (0.72 & 0.41) in Buccaneer anchovy were reasonable for current fishing effort due their short life span. Analysis of the results showed that CPUE in sardine and anchovy affected by reproduction and feeding processes. Results showed significant correlation between sardine CPUE and Sardine GaSI (P〈0.05, r= 0.499), similarly between anchovy CPUE and anchovy GSI (P〈0.05, r=-0.635). Further, significant correlation between sea surface temperature with sardine CPUE (P〈0.05, r=0.493), phytoplankton distribution (P〈0.01, r=0.560), anchovy GSI (P〈0.05, r=0.526), anchovy GaSI (P〈0.05, r=-0.500) and chlorophyll a (P〈0.01, r=0.780). Phytoplankton distribution with sardine GSI (P〈0.05, r=0.453), zooplankton distribution with anchovy GSI (P〈0.05, r=0.671), and chlorophyll with sardine GSI (P〈0.01, r=0.761) have significant correlation.
    Keywords: Fisheries ; Iran ; Persian Gulf ; Qeshm Island ; Bandar Lengeh ; Sardinella sindensis ; Encrasicholina punctifer ; Reproduction ; Feeding ; Growth and mortality ; Catch per unit effort ; Sea surface temperature ; Chlorophyll a ; Sexual maturity ; Plankton ; Naplius ; CPUE ; GSI ; Sardine ; Anchovy ; Coastal waters ; Surface ; Temperature ; Distribution
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  • 22
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    Comparison of growth and survival rate between import and native rainbow trout (Oncorhynchus mykiss) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25204 | 18721 | 2018-09-04 08:20:25 | 25204 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Rainbow trout is only species of cold water fishes which is rearing and propagation in Iranian fishery farms. Along of develop aquaculture industries increase request of best produces such as trout eyed eggs and larva. For this reason fishery farmers must be to provide that the way of import of other countries, such as: Denmark, Norway and France. As for this importance subject and unknown basic information about preference ratio for doing it, we needed to study about that to answer the suppliant questions. In this study carried out 3 test groups (3 treatments with 3 repetitions) and compared specific growth rate (SGR), condition factor (CF) and survival rate (SVR) between in this groups. Statistical analysis were significantly difference of increase rate of length and weight in French group with other groups (p〈0.05). Compared of main factors (SGR, CF, SVR) in French test group were significantly difference with other groups (p〈0.05). Survival rate percentage in Iranian treatments (SVR=%67) were significantly with the others (p〈0.05). This ratio respectively in Danish and French treatment was %54 and %48.
    Keywords: Aquaculture ; Iran ; Oncorhynchus mykiss ; SR ; CF ; SGR ; Growth ; Survival rate ; Rainbow trout ; Species ; Cold water ; Rearing ; Fishery ; Aquaculture ; Larvae
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  • 23
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    Study on physico-chemical characteristics of water in the southern of Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25563 | 18721 | 2018-10-05 16:51:28 | 25563 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: In this study, the characteristics of physico-chemical parameters of water done in coastal part of the southern Caspian Sea during 2010-2011 (four seasons) in 8 transects (Astara, Anzali, Sefidrour, Tonkabon, Noshahr, Babolsar, Amirabad and Torkman). For measurement of water quality parameters, 480 samples were collected in different water layers by using of APHA standards methods. According to modeling and statistical methods was used multivariate for evaluation of factor analysis (MFA) in general groups and discriminant analysis (MDA) for spatial and temporal with water quality parameters. The statistical procedure of MFA was used for data reduction and finally six factors selected with about 80.48 percent of total variances related to them. The statistical procedures of MDA were used for the role of spatial and temporal of water quality parameters in different water layer. The function one gave eight parameters (pH, salinity, EC, DO, NO^3-, water temperature, NH^4+ and N^-total) affording more than 80, 67, 80, 77 and 71 % correct assignations (return to the same transects) in spatial analysis. In all transects during four seasons, function one gave five parameters (water temperature, salinity, EC, NO^2- and NH^4+) to afford 100, 100, 100, 97 and 97 % correct assignations in temporal analysis. The results showed that, the average amounts in light penetration layers during four seasons were for EC(15.04±0.18ms/cm), pH(8.38±0.01unit), salinity (10.31±0.16g/l), DO(5.67±0.09ml/l) and water temperature (18.28 ±0.72°C). The average amount of nutrients for parameters such as organic and inorganic phosphorus, total phosphorus, NO^2-, NO^3-, NH^4+, N^-Total and SiO_2 were 0.50±0.02, 0.32±0.01, 0.82±0.02, 1.76±0.10, 0.10±0.01, 1.51±0.10, 46.82±1.80 and 8.60±0.29µM, respectively. The average amounts in dark layers during four seasons were for EC (15.46±0.38ms/cm), pH (8.34±0.03unit), salinity (10.70±0.35g/l), DO (4.55±0.13ml/l) and water temperature (9.62 ±0.16°C). The average amount of nutrients for parameters such as organic and inorganic phosphorus, total phosphorus, NO^2-, NO^3-, NH^4+, N^-Total and SiO_2 were 0.54 ±0.05, 0.33 ±0.02, 0.87±0.06, 1.92±0.21 , 0.08± 0.01, 1.55±0.25, 45.10±3.25 and 10.05±0.62 µM, respectively. The average amounts of light penetration in all transects during four seasons 4.49±0.38 meters. The maximum light penetrations during spring, summer, fall and winter seasons were 9, 8, 8.20 and 7 meters, respectively. The maximum water temperatures in light and dark layers were 32.36 and 11.50°C. Also, the maximum differences about thermocline temperature during summer and fall seasons were 19.6°C and 10°C, respectively in southern part of Caspian Sea. Data in spatial analysis were not significant (p〉0.05), it means if add or removed any transect did not change occurred. But in temporal analysis were significant (p〈0.01), and it means no way remove any seasons for a research work on Caspian Sea in southern part. In those transects with 100m depth. Water temperature is only highly effective parameter between other parameters. In function 1 between 3 functions in transects with 100m depth water temperature with 60% variance was the main role and effective between different seasons in depth part were very high.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Astara ; Anzali ; Sefidrour ; Tonkabon ; Noshahr ; Babolsar ; Physico-chemical parameters ; Nutrient maters ; Sea water ; APHA ; pH ; Salinity ; Temperature ; Nutrients
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  • 24
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    Using biotechnology and chemical approach extraction of chitin and conversion to chitosan from the cyst shells of Artemia urmiana (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25618 | 18721 | 2018-10-07 16:34:53 | 25618 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Chitin and chitosan are 2 very important products of biopolymer that enjoy high consumption in industry, but their production sources are very limited. In this study, Artemia urmiana cyst shells were obtained from previously collected and stored ones in Iranian Artemia Research Center. 20 kg of Artemia urmiana cyst shells were sampled, cleaned, separated, dried and transferred to Iranian Artemia Research Center Laboratory to extract their Chitin and chitosan. Their chitin and chitosan initially were extracted using optimized common chemical methods. Their properties were compared to 2 other types of Chitin and chitosan obtained from crab and shrimp manufactured by Vietnam and China, respectively. To determine their quality, elemental analysis device, infrared spectrophotometry, x –ray radiography, determination of viscosity , molecular weight, crystallinity percent, color, de stylization measure, empirical and molecular formulas were made. The results showed that the percentage of chitin obtained from Artemia cyst Shells in Chemical method was 28 ± 3 % by weight and efficiency into chitosan (grade steel relief) in this method was 50± 5%. To optimize the extraction procedure and the removal of proteins of chitin by biological practices that were done by sodium hydroxide in the chemical method, it was replaced by the bacterium Bacillus subtilis. And in the bio- phase of chitosan de steelation fungus Aspergillus niger enzyme was replaced instead of sodium hydroxide at high temperatures. The results showed that chitin and chitosan can be extracted from Artemia cyst shell using biological method and their characteristics included as in chitin 49.6% C, 8.2 % N, 7.5 % H, and 34.5 %O. Also the same levels for chitosan were 44.4 %, 8.9, 7.2 and 39.5 %, respectively. Their other quality characteristics were included chitin average molecular weight 4.9×10^6 Dalton, crystallinity percentage of 36.4, viscosity at 20°C 31 centipoise and its color was gray to brown. In the biologic method, the average molecular weight of chitosan, crystallinity percentage, viscosity at 20°C, were 5.1×105 Dalton, 94.5, and 18 centipoises, respectively. Also, its color was pale brown. Chemical structure of extracted chitin and chitosan from the shell of Artemia urmiana cysts were C_7H_12NO_4 and C_6H_11 NO_4c, respectively. The comparison of chitin and chitosan obtained from each chemical and biological method revealed that replacing biological methods instead of chemical methods is possible in achieving these products at suitable condition and better quality. This can eliminate the use of chemicals damaging the environment such as sodium hydroxide and decrease environmental pollution.
    Keywords: Biology ; Chemistry ; Iran ; Artemia urmiana cyst shell ; Chitin ; Chitosan ; Chemical ; Biological methods ; Crab shell ; Shrimp shell ; Biotechnology
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  • 25
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    Identification of qanats in Rafsanjan, Sirjan and Ravar cities for fish culture (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25229 | 18721 | 2018-09-06 06:14:35 | 25229 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Study and recognition of the Quanat water of Rafsanjan, Sirjan and Ravar cities has been beginning since the November of 2004. Among 228 Quanat chains were studied in these cities, 165 quanat chains had a discharge more than 10 liter per second (L/Se) which were sampled. In this research oxygen, pH, discharge, water temperature and air temperature were measured in two times and in the local place. Height from the sea level, longitude and latitude were measured (by GPS Machine) in one time and in the local place and other physical and chemical factors including No_2, No_3, Co_2, total hardness, NH_3 and So_4 were measured one time in the laboratory. In this manner, the condition of connection ways of considered Quanat with city was studied. With examination on the Quanat water in above said cities, all of Quant was within desired extents for aquaculture activities. Moreover in some of them, water temperature was higher than 20 degree centigrade that with some changes and in specific months can be used in aquaculture.
    Keywords: Biology ; Iran ; Rafsanjan ; Sirjan ; Ravar ; Fish ; Aquaculture ; Quant ; Water ; Oxygen ; pH ; Temperature ; No2 ; No3 ; Co2 ; NH3
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  • 26
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    Production of bacterial probiotic from trout (Oncorhynchus mykiss) for improvement of immune system and challenge to streptococcosis (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25649 | 18721 | 2018-10-08 08:09:48 | 25649 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This study was conducted for the first time and the effects of probiotic bacteria isolated from the gut of Rainbow trout (Oncorhynchus mykiss). In the first step, after the purification of the isolated bacteria, probiotic properties in vitro was assessed with injection to healthy fish and challenge with three species of pathogenic bacteria in culture media. The second phase of the bacteria isolated in 5 treatments (log 7, 8 and 9 of the lactic bacteria (LAB), Vibrio sp and Pseudomonas sp) and a control treatment on growth and survival, hematological parameters (RBC and WBC cells), immunology and physiology parameters tested during the 60 days of the above parameters after 30 and 60 days and finally at the end, the final assessment was Streptococcosis resistance in fish. The results showed that the isolated bacteria were able to enhance the growth parameters (weight, feed conversion, feed fat, protein efficiency ratio and specific growth rate) and survival. The results showed that log 8 LAB was significant difference with other treatments and control. When using log 8 LAB and Vibrio, the MCV, MCH and MCHC were decreased with no significant difference. The log 8 has the greatest effect on the amount of liver enzyme (AST), IgM and complement component C3, and had significant difference with other treatments. The challenge examination to Streptococcus iniae showed the highest survival in treatments with log 8 (96.66%), and Vibrio (93.33%) and then subjected to other treatments and control are also the least survival (25.38%). The conclusion of that study is the first probiotic properties of bacteria isolated from trout to changes in quality indicators are in particular the LAB bacteria and the second log 8 of LAB had significant positive of changes development and safety, and the fish are resistanted against Streptococcosis.
    Keywords: Aquaculture ; Health ; Iran ; LAB ; Rainbow trout ; Growth ; Immune system ; Streptococcosis ; Bacterial ; Oncorhynchus mykis ; Pathogenic ; Survival ; Hematological parameters
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    Extraction‚ purification, identification and amount verification of steroids in Sargassum glaucescens and Padina boergesni algae in Oman Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25642 | 18721 | 2018-10-14 02:40:17 | 25642 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Padina boergesenii is one of the most abundant brown algae distributed in the north of Persian Gulf and Oman Sea. In this study after sampling and preparation of Padina boergesenii by Chroform-Etanol (3-1) solvent and by Methanol has been extract. Separation and purification of the compounds was carried out using thin layer, general and inverse column chromatography, Cephadex and high-performance liquid chromatography (HPLC). Structural elucidation of the constituents was based on the data obtained from H-NMR, 13C-NMR, HSQC, HMBC, DEPT and Cephadex LH-20. The steroids compounds separated from above alga were identified as 22dehydrocholesterol (1), cholesterol (2), fucosterol (3), β-sitosterol (4), stigmasterol (5), ostreasterol (6) and two epimer of hyroxyestrol(7), based on their spectral data and from comparison with those previously reported in the literature.
    Keywords: Biology ; Chemistry ; Iran ; Oman Sea ; Persian Gulf ; Brown Algae ; Padina boergesenii ; Steroids compounds ; Extraction ; Purification ; Identification ; Amount verification ; Sargassum glaucescens ; Algae ; Abundant ; Sampling ; Chromatography ; Cephadex ; Dehydrocholesterol ; Cholesterol ; Fucosterol ; β-sitosterol ; Stigmasterol ; Ostreasterol
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  • 28
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    The feasibility of Gamasiab river area fish farming of rainbow trout (Oncorhynchus mykiss) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25665 | 18721 | 2018-10-14 03:04:11 | 25665 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This study was carried out about 15 months to determine suitable locations for the development of aquaculture across the Gamasiab river of Hamedan province in 2007 - 2008. Physicochemical parameters and macro benthic invertebrates fauna 15 and 13 stations respectively every 45 days and seasonally fish identify in 8 stations were performed according standard methods. The average water temperature was 12.8±3.7 oC, minimum and maximum dissoled oxygen were 2.6 and 12.5 mg/lit respectively during the study. The pH range was between 7.5-8.8 , the minimum and maximum Ec was 250 and 750 µmos/cm in Mehr and Tir. The transparency range was recorded about 1-103 F.T.U. The uder factors such as amount of suspended materials: 0.01-0.1 mg/l, amount of BOD5: 0.05-6.2 mg/l and COD: 30 (minimum) and 70.57 (maximum) were recorded. The total solids material (T.S.S) were less than 1 mg/lit. Totally 37 groups of bentic organisms were identified which 11 groups of them were appertained to pollution sensitive orders containing Ephemeroptera, Trichoptera and Plecoptera. During fish population studied 21 species belonging 5 family were identified. Based on obtained results of biological, ecological, economic and social parameters, 10 sites in 7 areas were suitable identified for fish rearing and 12 aquaculture programs (10 plan for fish rearing and 2 plan for increasing fish production of (Oncorhynchus mykiss). The minimum and maximum production capacity in mentioned places was estimated about 1675 and 3850 tons respectively.
    Keywords: Aquaculture ; Iran ; Hamedan province ; Gamasiab river ; Feasibility ; Complexes of fish farms ; Biotic and Abiotic factors ; Oncorhynchus mykiss ; Rainbow trout
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    Trout farms and other human activities effects on Cheshmehkileh river ecosystem in Tonekabon (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25792 | 18721 | 2018-10-13 08:54:26 | 25792 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Cheshmehkileh River and adjacent mountainous streams, play a strategic role as a historical axis for anthropogenic civilization, human welfare also habitat and migration pathway of commercial – biologic valuable fishes e.g. Caspian trout, Caspian kuttum, members of Cyprinidae family in south Caspian Sea drainage. Treats such as overfishing of Caspian trout and Red spotted trout stocks in mountainous headwaters, barriers construction and manipulations those are out of river carrying capacity developed by human activities, affected normal function of river as well. Sand mining big factories establishment next to the river, legal and illegal trade of river sediments, direct entry of Tonekabon landfill leakage into the river, development of Rainbow trout farms since 3 decades and huge effluents into the river containing dead fish and types of solids, escapement of cultured Rainbow trouts into the river, … are major minimum factors which needs basic information for integrating inclusively drainage management system. Cheshmehkileh River contains Headwaters of Dohezar (Daryasar & Nusha), Sehezar and Valamroud rivers during 13 monthly sampling phases between September 2009 and October 2010 based on macrozoobenthoses investigations by EPT, EPT/C EPA protocols, measurements of nominated physic-chemical and microbiologic parameters. Probability of Rainbow trouts escapement and invasion, existence, nutrition in Cheshmehkileh environment indeed investigated. Data analysis explained significant differences (P〈0.05) between groups of measured parameters in different sampling stations. Dendogram of clustered analysis based on consolidation of major biologic/ physic-chemical and microbiologic parameters, separated stations No. 1, 3, 2, 4 in one group and remained classified in different groups. Station 8 and 9 similarly separated which expressed general similarities according to Sehezar river environment which were differs in comparison with other stations. Station 11 separated according to its natural quality of water and environment. Similarities between station 10 to Sehezar river stations 8 and 9 expressed general influence of Sehezar River more than Dohezar River in Cheshmehkileh condition especially in station No. 10. High scores of EPT and EPT/C indices in upstream stations 1, 3 and 8 also low score of indices in stations 7, 13 and 6 expressed levels of environment quality between these groups of stations. Maximum average biomass of macroinvertebrates belongs to Trichoptera order in Cheshmehkileh River. Significant decrease of biomass in stations 11, 12 and 13 in comparison with other stations stated environment degradation in mentioned stations relevant to excessive sand mining as well. Pollution resistant groups of invertebrates significantly increased in downstreams against upstream stations. Also disappearing of Plecoptera order in station No. 7, 9, 10 and 13 stated low quality of environment in comparison with upstream stations. Confirmation of effects quality and quantity for point and non-point sources of imported pollutants require specific management considerations in order to present exploitations, pollutants control and emergencies for river monitoring in forthcoming years.
    Keywords: Aquaculture ; Management ; Iran ; Tonekabon ; Cheshmehkileh ; River ; Pollution ; Aquaculture ; EPTC ; Assessment ; Macroinvertebrates ; Chemistry ; Microbiology
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    Ecological study on the Persian Gulf Martyres Lake, Tehran (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25818 | 18721 | 2018-10-13 10:23:49 | 25818 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The ecological study of the Persian Gulf Martyres Lake carried out at the 5 stations between 2013 and 2014. This study identified 35 phytoplankton taxa. The diatoms taxa was dominated and their abundance recorded highest (2000 000 cell. L^-1) in the lake. The annual phytoplankton abundance was measured as 2500 000 cell. L^-1 during the study. The total nitrogen and water temperature were the significant a biotic parameters to increase cyanophytes abundance. Furthermore, 37 zooplankton taxa were identified. The Rotatoria abundance was dominated zooplankton. The annual zooplankton abundance was measured as 72 ind.l-1. Based on the CCA, there was no correlation between Rotatoria abundance and a biotic parameters. The study benthos showed Ephemeroptera and Diptera abundance were dominated; artificial bottom and lack of sediment and organic matters were the main reasons in decreasing of the benthos density. The Chitgar lake is the poorest lake in Iran due to low density of plankton and benthos organisms. The estimation of fish production was 123 kg/ha and for the lake was determaind 16 tonne. The finding display, invasive species was the main fish Chitgar lake that would be negative effect and increase eutriphication trend in the lake. Phosphorus parameter was limited parameters and trophy level recorded low due to high N/P ration in the lake. In overall the Chitgar lake situation is in Oligotrophic category with the low trophy level.
    Keywords: Ecology ; Iran ; Persian Gulf ; Chitgar Lake ; Phytoplankton ; Zooplankton ; Benthose ; Trophy ; Abundance ; Nitrogen ; Temperature
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    Investigation of chromosome and ploidy levels of rainbow trout produced from imported eyed eggs (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25825 | 18721 | 2018-10-13 10:43:05 | 25825 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Rainbow trout is the main cultural species of coldwater fishes in iran. Often, aquaculturists intend to breeding in order to production of lines with higher growth rate potential and disease resistant. Nevertheless in the country, no trout breeding programs, has been performed yet and most of the farms focused on the cultivation of the first (unbred) race. While European countries progressed in trout breeding techniques and production lines with higher growth through genetic manipulation (chromosomal number and type changes of fish) and/or selection and their fish products derived from this technology, including eyed eggs and so on have sold to other regions of the world (eg: Iran). In this study, some biological parameters including survival, growth, feed conversion ratio (FCR.) and chromosomal number of two juvenile groups from imported( group 1) and native(group 2) eyed fish eggs were compared. For chromosomal investigation, blood smear test and flow cytometry were performed. sults showed a significant difference (P≤5%) in growth rate of native fishes and French group Native fish feed conversion ratio (0.9) was significantly difference (P≤5%) from that of French fishes (1.15). Chromosomal analysis showed no difference in chromosome number in treatments and two fish groups were 2n chromosome. Based on the results, the fishes of group 1 had faster grow potential and gain weight in less time than that of group 2 and this has been achieved to go through the process of selection and feminization without any change in number of ploidy. Whereas the ability of native fishes in food efficiency ( lower FCR.) was better. However, the reduction of rearing period is the benefit and preference of cultivation of imported or origionally foreign.
    Keywords: Aquaculture ; Biology ; Iran ; Imported eyed egg ; Native fish ; Ploidy level ; Growth ; Rainbow trout ; Chromosome ; Species ; Breeding ; FCR
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    Induction of population triploid-interploid in rainbow trout (Oncorhynchus mikiss) using by indirect (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25827 | 18721 | 2018-10-13 10:47:16 | 25827 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Induced polyploidy is a suitable tool for producing sterile fish which made commercial benefits in the aquaculture industry. This study carried out in order to produce triploid-interploid population via mating tetraploid female with diploid male rainbow trout. Heat shock was used for making tetraploid population and the best temperature and induction time were examined. Result showed that highest mortality from 1 day after fertilization to emerging were in groups 6 of embryos (40.1%) and the lowest were in groups 3 (33 %). Flowcytometry results showed that some fish were polyploidy. Comparative analyze of genome levels in tetraploid fish to control fish (diploid) and hen as standard indicator, confirmed tetraploid fishes in this study. In conclusion we can state that heat shock induction for 7 minutes at 65 hour –degree after fertilization in 28ºC is optimum temperature for inducting tetraploid rainbow trout.
    Keywords: Aquaculture ; Biology ; Iran ; Sexual development ; Triploid interploid ; Tetraploidy ; Heat shock ; Flow cytometry ; Rainbow trout
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    A Study on necessary infrastructures used for breeding trout in Yasoj region (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25826 | 18721 | 2018-10-13 10:44:36 | 25826 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Rainbow trout is the main cultural species of coldwater fishes in iran. Often, aquaculturists intend to breeding in order to production of lines with higher growth rate potential and disease resistant. Nevertheless in the country, no trout breeding programs, has been performed yet and most of the farms focused on the cultivation of the first(unbred) race. While European countries progressed in trout breeding techniques and production lines with higher growth through genetic manipulation (chromosomal number and type changes of fish) and/or selection and their fish products derived from this technology, including eyed eggs and so on have sold to other regions of the world(eg: Iran). In this study, some biological parameters including survival, growth, feed conversion ratio (FCR.) and chromosomal number of two juvenile groups from imported( group 1) and native(group 2) eyed fish eggs were compared. For chromosomal investigation, blood smear test and flow cytometry were performed. sults showed a significant difference (P≤5%) in growth rate of native fishes and French group Native fish feed conversion ratio (0.9) was significantly difference (P≤5%) from that of French fishes (1.15). Chromosomal analysis showed no difference in chromosome number in treatments and two fish groups were 2n chromosome. Based on the results, the fishes of group 1 had faster grow potential and gain weight in less time than that of group 2 and this has been achieved to go through the process of selection and feminization without any change in number of ploidy. Whereas the ability of native fishes in food efficiency ( lower FCR.) was better. However, the reduction of rearing period is the benefit and preference of cultivation of imported or origionally foreign.
    Keywords: Aquaculture ; Iran ; Yasoj ; Genetic ; Rainbow trout ; Plan ; Relationship diagram ; Breeding
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    Economic and social assessment of fish farming in cages in the southern Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25830 | 18721 | 2018-10-13 10:49:04 | 25830 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This study aimed to assess the social, economic and fish farming in cages in the Southern Caspian Sea. The Caspian Sea is considered as one of the most important water resources of Iran, in which due to the appropriate weather conditions in the north of the country, has the potential for marine aquaculture. With the implementation of this plan would say that with respect to the proposed initial selection from Kheshtsar in Mazandaran province and Kiashahr in Guilan province with along coastline of about 250 km from the depth of 20 to 100 meters and an the area of about 2027km^2 has the cage aquaculture talent. Of course, at the depths of greater than 100 M by using the world technology procedure, there is also the possibility of the establishment of marine cage aquaculture. Now, with the regards of the above potential in the Caspian Sea coastline, it is expected at least 200 cages fish sites farming in with the establishment of 40 cages per site and produce 160,000 tons with creating of 1,000 direct jobs and 2,000 indirect jobs and income 2000 billion gross annual average net profit of 40% could be 800 billion in which will produce of proteins needed part of the country, creating jobs, welfare, prosperity and economy in the region. With the implementation of such national project, it is needed to establish pilot farms to reach the goals: environmental assessment studies, risk assessment, aquatic selection and the use of appropriate cage structures according to ecological conditions of southern Caspian Sea.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Mazandaran Province ; Guilan Province ; Cage ; Economic activity ; Profitability ; Rainbow trout ; Ecological conditions
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    Survey of potential adaptation (ion- osmotic) rainbow trout (Oncorhynchus mykiss) fingerling with Caspian Sea water (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25833 | 18721 | 2018-10-13 10:28:58 | 25833 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This study aimed to determine domain the adaptability of rainbow trout(Oncorhynchus mykiss) fingerlings in fresh water up to 20 per thousand (grams per liter) for providing facilities for growing this valuable species in the aquatic environment with salinity unconventional been completed. For this purpose the biochemical, bloody and physiologic parameters rainbow trout were studied to determine the adjustment range .Fingerlings fishes from cold water fish farm in the province Mazandran were prepared and for experiments were transferred to the Ecological Institute of Caspian Sea . Fingerlings with an average weight 31.56 ± 0. 07 SE g and average fork length 13.80 ± 0.15 SE cm, in 3 treatments in water with salinities (fresh, 13 and 20 grams per thousand) with a density of 15 numbers in polyethylene to 300-liter tank containing the 250 liters of water testing were introduced. Fresh water from Tajan rivers and water psu13 from the water Caspian Sea and water psu20 by mixing water the Caspian Sea and Sea salt was prepared . The daily amount to 50 percent of the of water tankss been replaced .During the experimental period was for 7 days and were not fed during the experiment.The water parameters was measured during the experiment included 6 ppm dissolved oxygen, pH equal to 8.2 and temperature 15.5 ° C . In the experimental period were not observed Losses in the experimental groups . The results showed that fish gill and kidney introduced in different salinities by making appropriate changes in chloride cells in the gills through increasing the number and the volume of these cells at the base of secondary blades and tubules in the kidney tubules to create greater interior space, are adapted to By changing salinity. Relatively parameters osmolarity, sodium, chloride, magnesium, cortisol, calcium, hematocrit, hemoglobin, number of red blood and white cells in water saltier than freshwater environment was higher (0.05〈 p, Duncan). Changes in hematological and blood plasma ionic parameters and vital organs Fingerlings indicates a willingness adaptability and the ability physiological adaptation fingerling was consistent with changes to environmental salinity brackish water .So, the factors measured with increasing salinity the uptrend that the range of variation for the osmolarite 449-281 mOsmol kg, for sodium, chloride, magnesium, cortisol, respectively, 211- 151, 165121, 3/3 7/0, 87. 53 mmol and the calcium 22-13 mg per dL. The measured values for hematocrit 32.2- 38.8%, hemoglobin 6.2 - 8.6 g per deciliter and the red and white blood cells, was respectively, 1.2-1.7×106 and the 15.6 -18.9×103.
    Keywords: Aquaculture ; Biology ; Iran ; Caspian Sea ; Adaptation ; Rainbow trout ; Gills ; Kidney ; Survey ; Oncorhynchus mykiss ; Fingerlings
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    Eutrophication trend of southeast Caspian Sea water (Mazandaran-Goharbaran) based on environmental and biotic parameters using single and multiple variables in order to feasibility of marine cage culture (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25839 | 18721 | 2018-10-13 10:48:08 | 25839 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The aim of this project is to study water quality and the trophic status at different months and depths in the southeast Caspian Sea-Goharbaran based on environmental parameters and nutrients during 2013-2014 in order the feasibility of fish cage culture. Results showed that water temperature, transparency, pH, dissolved oxygen, BOD5, COD, total alkalinity and TSS were varied 9.00-29.00C, 0.50-12.00 m, 8.05-8.74, 5.76-12.85, 14-101, 21-195 and 0.00-0.12 mg/l at the different months and depths, respectively. In addition, the values of NH^4+, NH_3, NO^2-, NO^3- and PO^4-3 were 0.007-0.051, 0.001-0.010, 0.002-0.015, 0.043-0.477 and 0.014-0.077 mg/l, respectively. Chl-a concentration was recorded as 0.060-8.02 mg/l. Scaled and unscaled trophic indices were equal to 3.42-5.52 and 2.61-5.85, respectively. The proper temperature for cultivation of salmonids species in this area began from October to March. The range of the standard of pH and dissolved oxygen level for fish farming cage were 7.80-8.50 and 〉5 mg /l that in current study results of pH and dissolved oxygen which were consistent in the current study. Various forms of nitrogen (ammonium, nitrite and nitrate ) at the different layers were acceptable and less than the threshold limit of Australia and New Zealand. The results of the nutrients concentrations compared with data from the reference years (1996-1996 ) in the Caspian Sea (Goharbaran region) showed that this ecosystem passed from oligotrophic status and shifted to mezotrophic and eutrophic condition. As a conclusion, although nutrients concentrations were acceptable in terms of fish farming cages in this region, some parameters such as chemical oxygen demand and transparency are not suitable for aquaculture industry. Meanwhile, based on various trophic levels, this ecosystem (Gohrabaran region) has a mezotrophic status with a high risk of eutrophication.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Goharbaran ; Physico-chemical parameters ; Water ; Fish cage culture ; Temperature ; Transparency ; pH ; Dissolved oxygen
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    Study on feasibility of rearing integrated native fish of Shizotorax zarudnyi with chianies carp on Sistan area (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25254 | 18721 | 2018-09-07 07:13:59 | 25254 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Present study carried out in 6 earthen ponds (3500 m^2 each) at the Zahak fish propagation and cultivation center. In July 2005 ponds were prepared and stocked with 1100 fish specimens with 40 g average weight. Stocking normative in two ponds (1 and 4) were 220 common carp, 110 grass carp, 715 silver carp, and 55 big head specimens. In the next two ponds (5 and 6) common carp reduced to 50% and Shizotorax zarudnyi was replaced. In the rest two ponds (7 and 8) common carp reduced to zero and totally replaced with Shizotorax zarudnyi. Manure and inorganic fertilizer were used during cultivation period. Carp was fed by concentrated food and grass carp was fed by alfalfa. At the end of the cultivation period (16 months) mean weights of common carp, grass carp, silver carp, big head, and Shizotorax zarudnyi reached 1201 g, 1572 g, 313 g, 486 g, 531 g respectively. Mean daily growth were 2.41 g, 3.19 g, 0.56 g, 0.92 g, 1 g respectively. Survival rate during cultivation period were 97.57%, 68.3%, 94%, 85.45%, 46.85% respectively. Biological parameters were recorded once a month. Other chemical and physical parameters such as air and water temperatures, oxygen, pH, twice per day and factors such as nitrite, nitrate, Ammoniac, transparency, salinity and phosphate were measured and recorded once per week. During cultivation period average pH was 7-10.34, water temperature was 0.5-33 °C, transparency was 12-115cm, salinity was 1.1-5 mg/l, oxygen was 2-10 mg/l, nitrite was 0.86-0.16 mg/l, nitrate was 0.06- 2.21 mg/l, and phosphate was 0.1-1.8 mg/l. Average total yields in the ponds 1 and 4 with standard normative for the region were 554, and 623 kg. In the ponds 5 and 6 with 50% Shizotorax zarudnyi were 514 and 518 kg, and in the ponds 7 and 8 with total Shizotorax zarudnyi replacement were 533 and 386 kg.
    Keywords: Aquaculture ; Iran ; Sistan region ; Fish culture ; Poly-culture ; Shizotorax zarudnyi ; Earthen ponds ; Specimen ; Common carp ; Grass carp ; Silver Carp ; Fertilizer ; Survival rate ; Transparency ; Salinity ; Temperature ; Nitrite ; Nitrate ; Ammoniac ; Tehran ; Iran
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    The effect of different dietary and bed treatments on earthworm (Eisenia fetida) for aquaculture (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25272 | 18721 | 2018-09-07 08:25:12 | 25272 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This experiment conducted to evaluate the effect of different dietary and bed treatments on earthworm (Eisenia fetida) for aquaculture. Concurrent with initial proliferation of earthworms, a pilot experiment also performed. In pilot experiment that conducted to become familiar with specialty and requirements of Eisenia fetida earthworms, lasted 4 months. The pilot treatments were including: 1) cow manure with covering its surface (control group), 2) cow manure (C.M.) without covering its surface, 3) C.M.+ 5% wind sand, 4) C.M.+30% saw dust, 5) urban compost, 6) C.M.+20% alfalfa meal, 7) C.M.+ 20% wheat bran and 8) C.M.+20% rice bran. After initial proliferation of earthworms and performing a pilot experiment, two trials designed. The first trial performed in outdoor environment, inside the plots dugged in the earh, with six treatments. The treatments were including: 1) cow manure(control group), 2) cow manure (C.M.) with vertical ventilation, 3) C.M.+ 30% saw dust (S.D.), 4) C.M.+30% S.D. with vertical ventilation, 5) urban compost+20% C.M. and 6) urban compost+40% C.M. The experiment lasted 6 months. In the end of trial in every plot, from 4 points, sampling for number of mature and immature earthworms, cocoons and the weight of earthworms were measured. The second trial performed in greenhouse by using wooden boxes. In this trial the treatments were including: 1) C.M. as control group, 2) C.M.+30% S.D., 3) C.M.+30% S.D.+ 10% alfalfa meal, 4) C.M.+30% S.D.+10% wheat bran, 5) C.M.+30% S.D.+10% rice bran, 6) C.M.+30% S.D.+10% soil, 7) C.M.+30% S.D.+5% fine sand, 8) C.M.+ 30% S.D.+1% lime powder and 9) C.M.+30% S.D.+200 ppm ammonium nitrate. Every treatment performed in 3 replicates. The earthworms reared in controlled condition for moisture and temperature, for about 4 months. The pH of treatments measured monthly by paper pH meter and by method of solution extraction. In the end of trial the number of mature and immature worms, cocoons and the weight of earthworms in every box were measured and the average weight of every earthworm calculated. The results of pilot experiment showed that covering surface the bed is necessary for maintaining moisture and darkness for earthworms. In wind sand treatment, the bed had sticky and compacted statues and appeared that the earthworms moved and activated difficultly. The saw dust treatment had a wet- spongy environment and the earthworms distributed well in it and their size was larger than control. Urban compost treatment unlike its suitable pH (equal 8), hardly accepted by the earthworms and they stayed in the corner of bed for more than one month. The wheat bran and rice bran treatments, both had sticky texture at the beginning but by spending time, their beds improved. The results of first trial showed the Eisenia fetida earthworms can survive and growth in outdoor environment even in cold season of winter. In this trial the highest total number of eartworms and cocoons and the total weight of earthworms was belong to control group, that had significant difference with the treatments C.M. + 30% S.D., urban compost + 20% C.M. and urban compost + 40% C.M. (p〈0.05). The performing of ventilation in experimental treatments had not significant effect on the number of mature earthworms, total number of earthworms and their total weight compare to control group. The urban compost was not suitable for rearing Eisenia fetida, although addition of 20 and 40 % of cow manure could improve it for usage by the earthworms. The results of second trial showed that, addition of alfalfa powder, wheat bran, rice bran and lime powder to cow manure had not significant effect on the total number and total weight of earthworms compare to control group. The usage of soil in treatment C.M. + 30% S.D. + 10% soil caused significant decrease in number of mature earthworms compare to control group (p〈0.05). The treatments including addition of saw dust and saw dust + fine sand to cow manure increased the number of mature earthworms, cocoons and total weight of earthworms compare to control group significantly (p〈0.05) and treatment of ammonium nitrate caused significant increase in total weight of earthworms (p〈0.05) .
    Keywords: Aquaculture ; Iran ; Diet ; Treatment ; Earthworm ; Eisenia fetida ; Aquaculture ; Dietary ; Ammonium ; Nitrate ; Temperature ; pH ; Mature ; Immature ; Growth
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    Ecological study on rearing shrimp ponds (Litopenaeus vannemei) in Choebdeh Abadan (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25276 | 18721 | 2018-09-07 07:59:49 | 25276 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Following to introduce Vannamei shrimp (Litopenaeus vannamei) into Iran aquaculture industries by fisheries organization its postlarvae were successfully reared in ponds in Bushehr and Choebdeh in Khuzestan. Due to activity of Shrimp site and importance of input and output water quality, this study was done in 11 stations including: two stations in Bahmanshir river, two station in C4 and C5 irrigation canals, six staions in active farms along C4 cannel (Yonesi, Mosavei and Salman Zadeh), and C5 cannel (Mohamadi, Khairi and Ashraf Por) and one staion in output draing were selected. Sampling were carried out from May2009 befor beginning stocking to harvest time in October 2009. Physico- chemical parameters and plankton sampled biweekly and benthic animals and heavy metals were sampled monthly. Some parameters such as pH and temperature by using portable multy parameters and others. Analyzed with standard methods. Heavy metals were measured by plarography method (797 Vamtro). Plankton and benthos samples were studied only in Bahmanshir station. According to results the maximum values for DO (11.1 ppm) PO4 (1.86 ppm) NO3 (8.4 ppm) and TSS (4992 ppm), pH (8.4) NO2 (0.18 ppm) BOD5 (9.06 ppm) were measured. In comparison to aquaculture water quality standards except for TSS in river stations others are located in allowable range. We didn’t observed any main changes in water quality from river to output drainage. The results of biotic parameters in river stations showed that diatoms were the most group of phytoplanktons. Zooplankton groups were Copepods, Rotifera and Protozoa. Tintinids and Nematoda larvae were the most abundant zooplankton. Benthic animals were included nine crustacean species and two annelid species. Isopods with 52% were the most and ant group.
    Keywords: Aquaculture ; Ecology ; Iran ; Khuzestan Province ; Abadan ; Choebdeh ; Bahmanshir River ; Vannamei shrimp ; Litopenaeus vannamei ; Water quality ; Abadan aquaculture site ; Rearing ; Shrimp ; Ponds ; Sampling ; Plankton ; Heavy metals ; pH ; Temperature ; Benthos ; Phytoplankton ; Zooplankton ; Copepods ; Nematoda larvae
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    Cold pasteurization of rainbow trout fillet (Oncorhynchus mykiss) with gamma rays and shelf life assessment at refrigerated condition (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25320 | 18721 | 2018-09-12 09:47:39 | 25320 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The present study was conducted to evaluate the effect of different doses of gamma rays on shelf life and fatty acids composition of Rainbow trout fillet (Oncorhynchus mykiss) during refrigerating condition. Quality and shelf life of non-irradiated and irradiated (0, 0.75, 1.5, 2.25, 3, 3.75 and 4.5 kGy) Rainbow trout stored at +4 C was investigated by measurement of microbiological, chemical and sensory analysis .Irradiation of fillets was performed by gamma rays from a Co60 source. Microbial counts for non-irradiated trout fillet samples were higher than respective irradiated fish. Total volatile base nitrogen (TVB-N) values was increased from 7 mg/100 g for non-irradiated to 52.7 mg/100 g after 15 days storage at refrigerator, whereas for irradiated fish lower values of 39.4, 37.6, 28.7, 25.6, 24.8 and 23.2 mg/100 g were recorded at 0.75, 1.5, 2.25, 3, 3.75 and 4.5 kGy, respectively (day 15).initial amount of peroxide value in non irradiated sampleswas 0.8 meq/kg and was increased to 7 meq/kg (7 day).Highest amount of peroxide after irradiation process was belonged to samples irradiated by 4.5 kGy (1.5 meq/kg). Also, total viable count of irradiated samples were decreased significantly (p〈0.05). On nutritional aspect, results showed that total saturated fatty acid concentrations increased significantly (p〈0.001) with increasing irradiation dose , so that the control sample and fish muscles that irradiated with 4.5 kGy had the lowest and highest amounts of total saturated fatty acids (20.454 ± 0.011% and 19.228 ± 0.040% , respectively). The amount of total polyunsaturated fatty acids (PUFAs) in irradiated samples were significantly lower than control sample (p〈0.001) and amounts of total monounsaturated (MUFAs) were significantly higher than control samples (p〈0.001). The results were indicated that the highest content of MUFAs and PUFAs were in samples irradiated with 3.75kGy (37.783± 0.092 %) and control samples (37.677± 0.104 %), respectively .The lowest level of MUFAs and PUFAs were in control samples (36.596 ± 0.024%) and 4.5 kGy (36.459 ± 0.047%), respectively. Altogether, results of this study were determined that irradiation treatment changes significantly the fatty acid composition especially polyunsaturated fatty acids in rainbow trout muscle (P〈0.001). Overall, irradiation of fillet by 3.75 kGy had best results on increasing shelf life of fillet at refrigerator (between 11-15 days) and had no advers effect on fatty acid composition of Rainbo trout fillet.
    Keywords: Aquaculture ; Fisheries ; Iran ; Irradiation ; Rainbow trout ; Fillet ; Shelf Life ; Fatty acid ; Refrigerator ; Pasteurization ; Oncorhynchus mykiss ; Gamma Rays ; Samples
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    The study of annual cycle of gonads developments and sex steroids of gray mullet, Mugil cephalus in Golestan Province (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25319 | 18721 | 2018-09-11 09:09:09 | 25319 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Grey mullet, Mugil cephalus is one of the most famous and valuable fish on the fisheries and aquaculture industry. This species is the most suitable to introducing the southern, northern and central regions of our country due to high resistant to large variation of water salinity and temperature. On the monthly sampling period biometric data including total length and total weight recorded and length and weight classes for every sex were determined. To understanding of physiological functions and sexual development of reproductive organs, this investigation carried out with Grey mullet imported from Hong Kong by 1993. Findings of this research can be used for management of artificial breeding of this species. Daily variation of pond water temperature was recorded. Sex steroid hormones and other plasma metabolites such as Ca^2+, cholesterol, triglycerides and total proteins were measured by blood sampling. Gonad samples were prepared in order to histological studies. Oocyte growth was studied by measurement of egg diameters. Measured values of cholesterol, triglycerides of blood plasma revealed that these metabolites have seasonal variation and severely deposited on the summer and mobilized on the spawning seasons. Findings of this research suggested that egg diameters, values of sex steroid hormones (estradiol and progesterone) and Calcium concentrations of fish blood plasma shortening increased a long with day length shortening.
    Keywords: Aquaculture ; Iran ; Golestan province ; Mugil cephalus ; Sex steroid hormones ; Plasma metabolites ; Gonads ; Gray mullet ; Mugil cephalus ; Fisheries ; Aquaculture ; Species ; Salinity ; Temperature ; Sampling ; Estradiol ; Progesterone ; Calcium
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    Survey of diversity, distribution, abundance and biomass of macrobenthic fauna in the southern Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25329 | 18721 | 2018-09-13 12:35:03 | 25329 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This project were investigated seasonally from march 2008 up to one year, in southern Caspian Sea iranian coast /Sampling was carried out at 8 stations of between 5 and 100 m depth and taken in triple with a 0/1m² van Veen grab/Total twenty four species and one family and one order were identified, presenting low dominance and frequency/ polychaeties 56%,oligochaetes 39% bivalves 〈1% amphipodes1% Cumacea and diptera and cirrepedia %1 of whole macrobenthos compositions/ Polychaetes and oligochaeta dominated, representing 95% of the whole fauna/Average of species were 11/8 species per grab/max 21 species / grab were found in Tonkabon stations at 100 meter depths and minmum 4 species/grab in 20 m in Bandar anzali /Maxmiun density were 4783 n/m^2 in spring and minmum 3177 n/m^2 in winter/Highest density were recorded in Amirabad stations and lowest in nooshar/ Maximum density showed in east, mimun in middle area of sea The biomass of benthos adventages in west/Average biomass of whole area were 6/932 gr/m^2/Mean total organic matter were 3/11±1/1 and silt- clay 62/92±6/12precent and Mean dissolve oxygen and salinity were 8/3±0/15 mg/l and salinity 12/2 ±0/4 ppm and water tempreture were 14/9±0/18ْC/ Mean grain size, fine sand, very fine sand, silt and clay contents were among the main factors related to the patterns of macrofaunal distribution, density and diversity and dominance of trophic groups.
    Keywords: Ecology ; Iran ; Caspian Sea ; Tonkabon ; Bandar Anzali ; Macrobenthos ; Disturbiotion ; Diversity ; Productions ; Survey ; Diversity ; Abundance ; Biomass ; Macrobenthic Fauna ; Sampling ; Species ; Polychaeties ; Oligochaetes ; Bivalves ; Dissolved oxygen ; Salinity ; Temperature
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    Hydrology, hydrobiology and environmental pollution study of the southern of Caspian Sea basin (10-100 meter depth) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25335 | 18721 | 2018-09-13 12:35:23 | 25335 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This study was conducted to study of Hydrology, Hydrobiology and Environmental pollution in the southern of the Caspian Sea. 33 stations were selected from Astara (in the west) to Hassan gholi (in the east). In each station, sampling carried out at suface, 10, 20, 50 and 100m depths. Sampling was performed during four seasons in 2004. Sampling stations also divided into three regions, including west (from Astara to Ramsar), middle (from Ramsar to Sari) and east (from Amirabad to Hassengholi). The results showed that maximum of water temperature was 24 ?C at summer in the eastern region while the minimum of water temperature in winter season has been 9.7 ?c in the western region. The average of dissolved oxygen concontration in spring, summer, autumn and winter season were 6.9 , 6.6, 8.2 and 6.7 mg/l , respectively. The maximum value of salinity was observed in spring (13.55 ppt) and minmum value in winter (8.24 ppt). The average of total nitrogen concentration were season was 711, 884, 583 and 669 µg/l in spring, summer, autumn and winter repectively. The average of total phosphorus concentration was 27.9, 37.1 and 42.6 µg/l in spring, summer, autumn and winter, respectively. The average of dissolved silica concentration has varied from 167 to 923.6 µg/l. The five phyla of phytoplankton have identified. The most species diversity was observed in winter (70 species) but the low species diversity has obtained in autumn. The most phytoplankton population was in spring and summer seasons. The cell abundance of phytoplankton in different seasons was fluctuated from 3914*103 to 17337*103 ind/m^3. In addition, the phytoplankton biomas was varied 11.4 to 30.7 mg/l. Result showed that distribution of phytoplankton are varied at different areas, layer, seasons and stations. The average of cell abundance of zooplanktons was varied from 2023 to 12364 and/m^3 at different seasons. The mean of bioman varied from 9.40 to 43.48 mg/l. In this study, 17 species and one class of macrobenthos were identified. The frequency of Annelida and Biovalvia was higher than all macrobenthos. The average of macrobenthos was 1218±851 ind/m^2 and their biomass was 14±15 g/l. The average of PAHs concentration were 0.038, 0.028 and 0.21 mg/l in spring , summer and winter, respectively. The decending trend of heavy metal concentration were Fe〉Mn〉Zn〉Cr〉Pb〉Co〉Cd〉Cu at different seasons in 2004. The maximum concentrations of organochlorine pesticides (OCPs) components in three regions of sampling zone such as , -BHC (middle), β,-BHC (middle), DDT (east) and dieldrin (east part) were 88.3, 35.0, 74.3 & 79.1 g/l during spring, summer, fall and winter, respectively.
    Keywords: Ecology ; Iran ; Caspian Sea ; Astara ; Physo-chemical parameters ; Plankton ; Benthos ; Enviromental Pollutions ; Hydrology ; Hydrobiology ; Sampling ; Temperature ; Dissolved oxygen ; Dissolved silica ; Species ; Phytoplankton ; Biomass
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  • 44
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    The study of possible biotechniques and normative to production some species of Anostraca (Phallocryptus spinosa) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25363 | 18721 | 2018-09-14 07:46:07 | 25363 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This project was carried out in order to determine the hatching conditions and laboratory culture methods of Fairy Shrimps (Phallocryptus spinosa). Physico- chemical factors and phytoplankton of natural habitat of this organism was investigated in Khasellou region around Azarshahr – East Azarbaijan province. The results showed that occurance and growth of fairy shrimps in their habitates were beginning from early Aprill and their survival was decreased with increasing temperature, precipitation and increasing the salinity of their culture medium. Then they disappeared. Their habitat phytoplankton contained 3 phylla and 10 genera, from which green algae enjoyed the greatest density in May. The pool salinity was around 20-17 g/l, when metanauplii was observed in early spring. In the pool containing and without fairy shrimps the oxygen concentration was high (9 mg/l) and low (2.9 mg/l), respectively. In 9 prawn ornamental fish genera, feeding with fairy shrimp showed a significant higher fecundity and hatching percentage compared to manual diets (cow liver, spinach). In all groups fed with fairy shrimp the duration of spawning time were reduced from 15 to 8-10 days and the color was enhanced. The means of cyst number per captured female also, cyst, decapsulated cyst and Naupilli diameters were mesured 142.9±19.0 cysts, 273.2±4.9µ, 242.4±3.8µ and 542.6±27.0 µ, respectively. The hatching rate was increased by reducing temperature and salinity of their culture mediums from 25 to 15 ◦C , and 28 to 18 g/l, respectively. The highest hatching was observed on 5th day at 15 ◦C and 18 g/l (5, 33 %). In addition, low but multiple hatching was observed. Therefore, water temperature and salinity affected hatching rate in this species. The hatching success of Phallocryptus spinosa cysts was better when collecting cysts together with dry sediments of their habitat without separation sensitive cysts. In addition to cold keeping and freezing, the hydration – dehydration method caused more diapause deactivation for hatching induction. The laboratory results revealed that the survival of fairy shrimps were highest at 15 ◦C (42%) and lowest at 25◦C (26%), respectively. Also, their life was inhibited in 30◦C. In different salinities, the mean survival rate were 26% (Salinity= 15 g/l) and 20% (Salinity= 25 g/l), respectively. The mean survival percentage of Phallocryptus spinosa cultured in natural habitat water (64.3%) was higher than aerated tap water (23, 3 %). Also, feeding with Nanochlorepsis caused higher survival rates. On the other hand, Beakers yeast caused higher mortality rates. Therefore, regarding to relatively high sensitivity of these species to physico– chemical and nutritional conditions of their culture medium, it is better to culture these species with expansion of their natural habitat in order to preserve their biodiversity, culture and increase stocking density with regard to environmental considerations .
    Keywords: Aquaculture ; Iran ; East Azarbaijan province ; Fairy Shrimps ; Phallocryptus spinosa ; Hatching ; Culture ; Biotechnique ; Species ; Anostraca ; Phytoplankton ; Growth ; Temperature ; Salinity ; Phylla ; Feeding ; Survival rate ; Mortality ; Biodiversity
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  • 45
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    Standard of insurance procedures and risk assessment manual for cold water fish culture regarding management and natural disasters (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25379 | 18721 | 2018-09-14 07:39:10 | 25379 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The total production of Rainbow trout fish aquaculture in 2009 had been 72515 t which was significantly increased (91519 t) in 2010. Despite of increasing trout fish production during last decade, absence conformity between suitable cultural and proliferation district of this fish species and geographical one, faced this industry harmfully to natural disasters such as flood, overflow and so on, and mismanagement due to human injustices. Hence insurance and supporting concept of can be having a determinant role in efflorescence and increasing production of this fish. The aim of this project is performing an insurance policy based on risk analysis model in propagation and cultural phases of rainbow trout aquaculture, separately. In this study, pay attention to standards in trout fish aquaculture procedure and determination of critical parameters on, are the first research step and recognition of risk factors in different aquaculture phases due to natural disasters and mismanagement, from the brood stock maintenance till reach to 3-5 g fingerling and finally market size fishes, are the following research steps. After determination of proportion percentages of each parameters regarding to arise the risk, analysis risks and risk management were done for trout fish aquaculture. Three provinces, Fars, Mazandaran and Charmahal Bakhtiary as poles production of trout fish were chosen as delegates of the whole Iran and insurance ability of trout fish hatcheries and aquaculture farms were done. Trout aquaculture standardization were obtained, natural disasters and mismanagement affected on aquaculture activities were recognized (Chapters 1 and 3) and due to importance of health and disease of trout fish, mortality causes and audit signs of also determined and analyzed (Chapter 2) . In Chapter 4, trout farms degraded and fix and natural disasters for trout fish brood stock, propagation hatcheries and cultural farms were analyzed and accounted. Finally, premium and recompense were accounted for hatchery and farm separately.
    Keywords: Management ; Iran ; Rainbow trout ; Risk Factors ; Risk Management ; Aquaculture Insurance ; Fish culture ; Cold Water
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  • 46
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    Ecological study on fisheries important rivers and bays in the south part of the Caspian Sea 2nd phase: Gorgan Bay (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25385 | 18721 | 2018-09-18 05:58:09 | 25385 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: In this study ichthyoplankton, phytoplankton, zooplankton, benthic and hydrochemistry parameters were studied. 9 out of 11 sampling stations were in the Gorgan Bay area and 2 of them were in the mouth of the Bay. Ichthyoplankton survey were carried out in 6 stations and the other surveys such as phytoplankton, etc were done in 11 stations in 2009. Gorgan Bay map and position of sampling sites are given at the end of material and methods section. Biomass and frequency of phytoplankton at different stations, months and seasons were calculated that are presented in Tables 1 to 3. Hydro-chemical parameters such as water temperature, air temperature, visibility, DO, BOD5, pH, Alkalinity (bicarbonate, carbonate and total), Hardness (calcium, magnesium), CL,EC, Salinity, N (nitrate, nitrite, NH_3), Phosphate were measured and results are shown Table 11. Biomass (mgr/m^3) and numbers (ind./m^3) of 47 genus of different phylum of phytoplanktons such as Cyanophyta, Chlorophyta, Pyrophyta, Chrysophyta and Euglenaphyta as well as different phylum of zooplanktons consist of Protozoa, Arthropoda, Rotifera and Cyliophora were estimated. Frequency of phytoplanktons (ind/m^3) was 131.2, 1.6, 65.3, 18.9 and 23.8 million respectively. Furthermore, their biomass was 308.4, 1.9, 1358.9, 295.1 and 124.7 mg/m^3, respectively. The maximum and minimum numbers were observed in Leptolingbaya belonged to Cyanophyta and Diatoms of Chrysophyta 84.3 and 0.025 million (ind/ m^3) respectively. The maximum and minimum biomass was observed in Gyrosigma (Chrysophyta) and Tetradron (Chloropyta), 12317.3 and 0.014 mg/m3 respectively. The average biomass of zooplankton was measured 531.74, 30.77, 225.07, and 96.26 mg/m^3 respectively and the total was attained 883.83 mg/m3 Table 5. The maximum and minimum biomass of all zooplanktons was estimated in November and July, 3446.23 and 6 mg/m^3 respectively. Furthermore, the maximum and minimum numbers of zooplanktons were found in September and July which were 48.4 and 0.002 millions respectively and the average number was 8.4 ind/m^3 Table 4. Also numbers and biomass (gr/m^2) of 11 families of benthos which inhabit Gorgan Bay such as Annelida, Mollusca and Arthropoda were investigated in separated stations and distinctive months. The maximum and minimum biomass of benthos was observed in January (42.91 gr/m^2) and March (0.6428 gr/m^2). The biomass was considerable in April, February, August and December (Tables: 4, 5 and 6).
    Keywords: Ecology ; Iran ; Caspian Sea ; Gorgan Bay ; Ecological study ; Fisheries ; Ichthyoplanktons ; Phytoplankton ; Zooplankton ; Benthic ; Sampling ; Biomass ; Temperature ; Visibility ; pH ; Alkalinity ; Salinity ; Phosphate ; Cyanophyta ; Annelida ; Mollusca
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  • 47
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    Application of earthworm (Eisenia fetida) in diet for rainbow trout (Oncorhynchus mykiss) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25395 | 18721 | 2018-09-20 11:33:11 | 25395 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This experiment was carried out to evaluate the effect of earthworm (Eisenia fetida) in diet for rainbow trout in complete random design for 6 weeks. Ten fish with mean 120 g was stocked in each replication.The earthworm at levels of 0, 25, 50, 75 and 100% was replaced (as fed) with fish meal in rainbow trout diet. The feed intake by weekly and weight gain of fishes by monthly were measured. Feed intake, weight gain, specific growth rate and feed conversion ratiothe were colculated, and the samples of fish meat were analysed for chemical composition including dry matter, crude protein, fat and ash. Results obtained showed that feed intake decreased significantly (P〈0.05) as earthworm increased. The diets contain 25% and 50% earthworm showed no significane differences compared to control for weight gain, specific growth rate and feed conversion ratio (P〉0.05). The chemical composition analysis of fish meat showed that there were no significant effects between control and 25% and 50% earthworm in diet for dry matter, crude protein and ash but, fat content decreaed as earthworm increased significantly (P〈0.05). This experiment showed that earthworm (Eisenia fetida) can be used in rainbow trout diet up to 50% instead of fish meal.
    Keywords: Aquaculture ; Iran ; Earthworm ; Diet ; Rainbow trout ; Growth ; Body chemichal composition ; Eisenia fetida ; Fishmeal ; Weight gain ; Growth Rate ; Samples ; Oncorhynchus mykiss
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  • 48
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    Feasibility study on semi intensive polyculture of chainess and Indian carps in I.R.IRAN (Khozestan province) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25424 | 18721 | 2018-09-23 16:21:43 | 25424 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: In order to diversify the production of carp (Cyprinid) in Iran, in 2010 September, 15,000 pieces of fry from three species of indian major carp were arrived to Iran. After subtraction of the dead fishes, finally 10,500 fry were introduced to 700 m2 ponds with a density 3,500 pieces, which includes 46. 7 % Catla, (Catla catla), 35 % Rohu (Labeo rohita) and 18. 3% Mrigal Mrigal (Cirrhinus mrigala). Primary average weight and length of these fishes were 0.3±0.0 g and 23.6±0.75 mm for Rohu, 0.1±0.0 g and 33.26±0.35 for catla and 0.16±0.01 g and 34.8±0.96 mm in Mrigal respectively. Fish were reared in the Khuzestan province's climate with at least 7°C and a maximum temperature of 35°C. The culture period was 6 month. Once every 45 days by using signebeach net samples were taken from ponds and biometry tests were done. The maximum net production was observed at 50% situated treatment, with average weight 723.3 kg per 1700 m^2 ponds (4254.9 kg ha). There is no significant differences between experimental treatments with each other and with control (P 〈0. 05). Mean final weight of Rohu in 3 experimental treatments were 1136, 1129, 1054 g and in Catla and Mrigal were 726,902, 839 g and 773, 788, 894. There was not significant diference between them (P〈0.05). Survival rate in Rohu, Catla and Mrigal was 92%, 97% and 86% respectively. The maximum specific growth rate (SGR) of fish related to Catla (1.92) and the minimum SGR of fish was observed in Mrigal (1.45). In accordance with the results of this study, the Indian carp fishes can able to adapt, grow and reach to market weight in the Khuzestan province's weather conditions.
    Keywords: Aquaculture ; Iran ; Khuzestan province ; Indian carp ; SGR ; Labeo Rohita ; Cirhinus merigala ; Catla catla ; Chainess crap ; Cyprinid ; Species ; Temperature ; Survival rate
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  • 49
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    Effect of different heating treatments and kind of filling media on the amounts of some mineral elements (iron, zinc, copper, calcium and sodium) of silver carp (Hypopthalmichthys molitrix) during canning (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25429 | 18721 | 2018-09-23 16:29:13 | 25429 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: In this study, first, the influence of three precooking methods (steaming, oven-baking and microwave-cooking) on the contents of mineral elements-copper, zinc, iron, calcium and sodium-of silver carp (Hypophthalmichthys molitrix) was evaluated. Determination results of evaluated elements in raw fish and cooked samples were in range 3.05 to 4.19 for copper, 71.45 to 82.85 for zinc, 32.18 to 40.70 for iron, 425.6 to 529.46 and 315.5 to 534.76 for calcium. Results showed different precooking treatments had no significant effects on the amounts of mineral elements such as copper, zinc, calcium and sodium. The Iron content in the samples subjected to microwave cooking increased. With doing multivariate analysis, on comparing the raw and precooked fillets, steam cooking found to be the best precooking method on retain mineral elements. After choosing the best precooking method, for evaluating the influence of different filling media including sunflower oil, soybean oil, olive oil and brine, on the quality of canned silver carp, amounts of mineral elements(copper, zinc, iron, calcium and sodium) and microbial indices(total counts, thermopile count and clostridium) and sensory indices (color, smell, taste and texture) was surveyed. Assessments of microbial indices showed no microbial activity in canned products. After canning amounts of sodium increased in all the treatments. Except for iron, using soybean oil had no significant effect on the contents of other elements. The highest levels of iron and copper were observed in olive oil canned samples sterilized at 130°C. Sensory evaluating showed, kind of filling media had no significant effects on sensory indices such as taste, smell and color of canned samples. The texture of soybean oil canned samples and the quality defects of olive oil and brine canned samples had better condition than other treatments. In the last step, canned silver carp were proceed under three different temperatures (115°C, 120°C and 130°C) with equal lethality value (Fo=7min), then sensory indices and amount of mineral elements were compared. Results showed, the contents of iron, copper, sodium and calcium were changed in soybean oil canned sample. The amounts of copper and sodium in sunflower oil canned sample subjected to different heating regimes showed significant variation. The highest amount of copper was observed in the canned samples subjected to 130°C heating regime. Results showed contents of iron and copper of olive oil canned sample subjected to 120°C and 130°C heating regimes were higher than sample subjected to 115°C heating regime, while the zinc and calcium contents had no variation. In brine canned samples the highest amounts of copper and iron was obtained after sterilization in 120°C. Results of sensory evaluation showed different heating regimes had no significant effects on the indices of taste, smell, and color of products. Doing 130°C heating regime in brine canned samples led to obtain the better tenacity of texture. While this heating regime caused to increase the quality defects of soybean oil canned samples as a result of existence of hard parts of bone.
    Keywords: Chemistry ; Fisheries ; Iran ; Precooking ; Filling media ; Heating regime ; Sterilization ; Silver carp ; Mineral elements ; Hypopthalmichthys molitrix ; Samples ; Sunflower oil ; Soybean oil ; Silver Carp
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  • 50
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    A study on physico-chemicals parameters, environmental pollutants, bacterial and macrobentos distribution at the surface waters, undergrand waters and wastewater of Mangol Dam basin (Mazandaran province) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25452 | 18721 | 2018-09-26 09:35:53 | 25452 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: At the present study, the water quality parameters monthly, environmental pollutants (pesticides, heavy metals and surfactants) seasonally, macrobenthos and bacteria (monthly) were done 2009 t0 2010 that located in Mangol Dam with longitude and latitude 52° 22' 49" N and 43° 15' 43" E, respectively. All water samples were collected from under-groundwater, surface water and wastewater in 12 stations and the results are as follow: The mean concentrations of DO, BOD_5, COD, HCO^3-, CO_3^2-, TA, Ca^2+, Mg^2+, TH, TSS, TDS, Cl^-, PO4^3-, NH^4+, NO^2- and NO^3- were determined about 11.86±0.49, 5.95±0.11, 4.96±0.59, 28.1±6.1, 14.34±1.64, 36.30±2.33, 64.66±2.34, 28.84±1.52, 271.62±8.41, 0.37±0.03, 0.45±0.02, 28.02±1.96, 0.06±0.01, 0.010±0.001, 0.01±0.001 and 0.96±0.03 mg/l, respectively and also temperature, pH and EC were 12.7±0.61°C, 8.00±0.02 unit, 0.57±0.02 ms/cm in all samples. The surface water quality of Haraz River and its tributaries were suitable for usual applications such as aquatic matters, but based on BOD_5 need to more attention for human usages. The water quality index (WQI) of surface water was classified in group II at all stations that shows serious water quality changes due to domestic and agriculture sewage. The groundwater was affected by different matters that caused change of water quality parameters. The wastewater was classified from good to moderate based on BOD5. The quality of wastewater was declined at down-stream with decreasing DO and pH and increasing BOD5 pollutants. The mean concentrations of aldrin and endrin were about 0.66 ± 0.59 and 0.71 ± 1.07 µg/l, respectively. The maximum seasonal concentration of organochlorin pesticides (OCPs) were detected in fall, winter and spring for endosulfan sulfate, γ-BHC, heptachlor, endrin aldehyde compounds about 2.85, 0.34, 0.14 and 0.14µg/l, respectively. The maximum seasonal percentage range of OCPs in fall, winter and spring for endosulfan sulfate, DDD and β-BHC were 42, 25 and 25 %, respectively. The maximum seasonal concentrations during fall, winter and spring were detected in Sorkhroud and Polechelave stations for only Fe about 1.48, 3.3 and 8.22 µg/l, respectively. Also for Hg in spring was determined about 17.2 mg/l. The mean concentration of Zn, Fe and Hg in water sampled were 0.23 ± 1.01 and 1.21 ± 2.00 µg/l, respectively and 4.65± 6.38 mg/l. The maximum concentration of linear alkyl benzene sulfonate (LAS) in summer was 971µg/l. The mean concentration of surfactant during fall, winter, spring and summer was 418.62 ± 52.16 µg/l. The maximum seasonal percentage ranges of surfactant during fall, winter, spring and summer in all stations of sampling were 76, 76, 100 and 100 %, respectively. Tottaly 15 families of macrobenthos were identified in Haraz River. The maximum mean of abundance and biomass in Sorkhroud and Nour-roud stations were 1177±256 ind/m^2 and 3609±935mg/m^2, respectively. During Seasons, the maximum mean abundance and biomass were detected in fall and winter with 1185±444 ind/m^2 and 3891±849 mg/m^2, respectively. Water quality were classified by Hilsenhoff Biotic Index such as Sorkhrood station as "Very Poor", Isar town station as "Fairly Poor" and Karesang and Nor-roud stations as "good" qualification. The maximum of total coli forms and fecal coli forms in surface water were observed in Sorkhrood about 4.9 and 2.3CFU/100ml, respectively. The maximum of total coli forms and fecal coli forms in groundwater were observed in Halomsar and Absak stations about 4 and 1.7CFU/100ml, respectively. Maximum of total coli forms and fecal coli forms in wastewater were observed in Nor-roud station about 3.1 and 1.6CFU/100ml, respectively. The Ovary of nematode in wastewater were observed in Nor-roud and Isar Town stations about 30 through 124, respectively.
    Keywords: Ecology ; Iran ; Haraz River ; Mazandaran province ; Mangol Dam basin ; Physico-chemical characteristics ; Surface water ; Ground Water ; Wastewater ; Pesticides ; Heavy Metals ; Surfactants ; Macrobenthos ; Coliforms ; Nematodes ; Environmental pollutants ; Bacterial ; Temperature ; pH ; Biomass
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  • 51
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    Survey of diversity, distribution, abundance and biomass of macrobenthic fauna in the southern Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25496 | 18721 | 2018-10-05 07:39:50 | 25496 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The Sampling of macrobenthic communities in southeastern Caspian Sea, Iranian coast, were investigated seasonally from April to March 2012. Sampling was carried out at 8 transect as named Astara, Badar Anzali, Sefied rood, Tonekaboon, Nooshahr, Babolsar, Amirabad, Gomishan.In each transect were choice 8 stations at 5m, 10m, 20m, 50m, 100m depths respectively. Samples were taken in triplet with a 0.1m^² van Veen grab.Totally 28 macrofaunal species belonging to 10 families were identified as gamaridae (9species), corophidae (3species),psudocumidae(8 species), amphartidae (2species), balanidae, chironomidae, cardidae scorbicolaridae, nereidae, sponoidae, each one species. Polychaetes were dominated, representing 72.5% of the whole fauna while while (equal 62.4% of total abundance and 5/1%biomass) were belong to Streblospio spp. from Spionidae family. Cerastoderma lamarcki belong to bivavia consist of 2/3% total macobethos abundance and 73% biomass. Total abundance of macrobenthos of middle area were higher than east and west area in southern Caspian Sea. Average benthic population density (no/m^2) with (SE) was 4581(444 ). The benthic biomass (mg/m^2) with an overall mean of 12. 125(02). Spatial and temporal variations of benthos. In the present study showed, maximum abundance Macrobenthos 17855(5543) no/m^2) were recorded in 20 meter depths at Nooshahr trancect , while minimum 841(109 )in 10 meter depths at Amirabad trancect p〈0.05. Maximum biomass 135.10(126) g/m^2 were obtained at 10m depths in Astara minimum 0.51(0.4) ) g/m^2 at 5m depths in Nooshahr transect . maximum were obtained in winter and minimum in summer Number of species varied 3 to 18 , Generally, minimum species diversity were obtained at 20 m depth in Tonekabon transects and the maximum value was observed at 100 m depth of sefeidrood variations physicochemical factors were between salinity 12.5to12.9ppt ,temperature 8-19˚c and PH 8.1 dissolve oxygen 69-101mg/li. Total secondary production calculated, according ,104/996g AFDW/m^2/yr.Maximum were recorded in 10meter depth (30/381AFDW/m^2/yr) and minimum at 50 meter depth(11/007g AFDW/m^2/yr) respectively. Average total organic matter were 3/18 percent in sample periods. Percent of total organic matter (TOM) were low in 5 and 10 m depths and increased toward offshore depths. TOM percent was 1/67(0.11 ) at 5 m depth and increased to 4/38(0.13 )in 100 m depth. Average total silt &clay were 68/63(2.97) percent in sample periods. Positive significant relation was between silt &clay and total organic matter. Macrobenthos community reduce in high silt clay and high sandy contents. Due to increase of streptolosbio.spp, monitoring program for understanding effect of this organism on ecosystem and on fish feeding is necessary.
    Keywords: Ecology ; Iran ; Caspian Sea ; Astara ; Badar Anzali ; Tonekabon ; Macrobenthos ; Diversity ; Distribution ; Abundance ; Biomass ; Secondary production ; Species ; Gamaridae ; Corophidae ; Psudocumidae ; Amphartidae ; Balanidae ; Chironomidae ; Cardidae ; Scorbicolaridae ; Nereidae ; Sponoidae ; Salinity ; Temperature ; Streptolosbio.spp
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  • 52
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    The creation of genetic basic population of rainbow trout (Onchorhynchus mykiss) based on study of genetic variation in brood stocks using microsatellite (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25509 | 18721 | 2018-10-05 08:39:09 | 25509 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: In order to perform the project, 446 samples of rainbow trout (Onchorhynchus mykiss) from 24 different regions in Iran were collected. About 2-3 g of caudal fin samples was collected from each specimen and preserved in absolute ethyl alcohol and then transferred to the genetic laboratory. Genomic DNA was extracted using the phenol-chloroform method and then DNA content and quality was determined using spectrophotometry and agarose gel electrophoresis, respectively. Polymerase Chain Reaction (PCR) of genomic DNA fin samples was carried out using 10 pairs of microsatellite primers. All PCR products were electrophoresed on 6% polyacrylamide gel and stained with silver nitrate. Following the scoring of alleles, all parameters including allelic frequency, effective number of allele, observed and expected heterozygosity, shanon index, measurement of similarity and genetic distance and Hardy-Weinberg equilibrium, Fst , Rst and gene flow were calculated using AMOVA analysis in the GenAlex and Popgene programs. The results showed that 8 pairs of microsatellite primers were polymorphic. In total, 50 alleles were determined with the range size of 64-280 bp. The locus omyf had maximum number of allele (26) and loci OTSG 474 and Strurruta58 had minimum number of allele (5). The observed heterozygosity was between 0.86 and 0.964. Hardy-Weinberg departure was observed for all loci from farms 18, 15, 4, E20 and 21 and were disequilibrium (P〈0.05). The farms 14, 8, 7 and 6 were equilibrium at 3 loci, but showed disequilibrium in other loci. The other farms were equilibrium at 1 or 2 loci and disequilibrium at 8 or 9 loci. The FST results showed that maximum FST (0.24) were between farms 1 and 11in which had minimum of gene flow (3.7). Minimum FST (0.04) were between farms 8 and 9 in which had maximum of gene flow (346). Based on the results of AMOVA analysis, significant differences were detected between all farms (P〈0.01). Furthermore, based on Nei 's standard (1972) maximum genetic distance (0.89) were observed between farms 2 and 11 and maximum genetic similarity (0.15) were detected between farms 3 and 4. This result suggests that the unique genetic variation of rainbow trout in hatchery farms of Iran represents a highly valuable genetic resource and provide useful information for creating a based population in the future breeding programs.
    Keywords: Biology ; Iran ; Genetic ; Population ; Rainbow trout ; Onchorhynchus mykiss ; Brood stocks ; Microsatellite ; Samples ; Specimens ; DNA ; PCR ; ANOVA
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    Possibility of the pond culture of Asian sea bass, lates calcarifer in Gwadar farm , Sistan & Baloochestan (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25578 | 18721 | 2018-10-06 17:39:33 | 25578 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: In the present study, the possibility of marine culture of Asian sea bass in the shrimp earthen ponds located in the Gwadar region (coastal area of Oman Sea, southeastern of Iran) was investigated. To this end, Asian sea bass frys reared for 7 months including 1 month nursery and 6 months growth-period. During the culturing periods, fish were feed ad libitum by various commercial rainbow trout diets based on fish growth stages. After nursery period, the average weight of 11.05±1.5 g and survival percent of 65 % were obtained for fish. During the growth-out period, the average weight increased continually from 31.4±4.7 g in late June to 252.1±30 g at the end of growth-out period in late Dec. Also, the highest values of monthly weight gain yielded during August, Sept and Oct when fish had the average weight range of (76.9±14.4)-(188.1±12.8) g. The values of monthly feed conversion ratio (FCR) were low and similar from July to Oct and then increased in Nov and Dec. The values of total monthly feed intake (FI) increased continually from July to Oct and then with a decrease in Nov, increased again in Dec. The values of survival percent after nursery and growth-out period were 65 % and 66% respectively. Also, the average of monthly FCR, FCR at the time of fish harvesting, total FI and total harvested biomass were 1.36, 2.5, 630.5 kg and 325 kg. in conclusion, the pond culture of Asian sea bass with marine water results appropriate survival and growth rates so that fish (11.05±1.5 g) obtained marketable weight (252.1±30 g) during 6 months culturing period. It seems that first-half of year in Gwadar area is more suitable for sea bass culture since this time periods results better FCR ( fed by rainbow trout feed) and has economic justification. Also, with regular sorting operation and providing of species-specific feed for Asian sea bass can increase the Production per Unit Area.
    Keywords: Aquaculture ; Iran ; Gwatr ; Sistan and Baloochestan ; Chabahar ; Lates calcarifer ; Asian sea bass ; Culture ; Pond ; Lates calcarifer ; Rainbow trout ; Survival rate ; FCR
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    Quantity evaluation and identification of pathogenic bacteria in water pond and cultivated Cyprinid fish enrichment by natural (cow dong) and chemical fertilizer (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25570 | 18721 | 2018-10-06 11:30:10 | 25570 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The aim of this study was comparison the effect of cow dung as organic fertilizer and chemical fertilizer on microbial flora, fish and human pathogenic bacteria and physico-chemical parameters of cyprinus fish pond water. The water samples were collected from two ponds, pond 1 was fertilized by cow dung and pond 2 was fertilized by chemical fertilizer, respectively from May to October 2011. . The aerobic and anaerobic bacteria were enumerated in TSA by serial dilution of the sample, followed by conventional pure plate method. Coliforms bacteria were similarly isolated on Chrom agarTM ECC. Moreover, the water samples were used to analysis of BOD, dissolved oxygen, conductivity, transparency, total alkalinity, total dissolved solid, total hardness, total nitrogen and total phosphorous. Temperature and pH were measured by thermometer and portable pH meter in place of ponds. For isolating of fish and human pathogenic bacteria, thirty six fish were collected from pond 1 from July to October and then sampled from their liver and kidney and inoculated in blood agar. The mean of total count of bacteria and total coliform count in cow dung sample were 4 × 104 ± 1200 and 4700 ± 300 CFUmL^-1 respectively. The mean of water total count bacteria of pond 1 were significantly higher than the water of pond 2. The water total count bacteria of pond 1 were significantly increased in August, but water total count of pond 2 was not shown significant variation from May to October. The results showed that Pseudomonas, Klebsiella, Enterobacter, Yersinia and E.coli were isolated from water of pond 1 but just Pseudomonas and E.coli were isolated from water of pond 2. Moreover, the human pathogenic bacteria shuc as Shigella and Salmonella were not isolated from of water and fish samples from pond 1. In this study, the bacteria growths were negative in all of the inoculated plate from fish liver and kidney. The total hardness, total phosphorus, total dissolved solid and conductivity of the pond 1 water were significantly higher than the pond 2 water. But the BOD, dissolved oxygen and transparency of the pond 2 were significantly higher than the pond 1 water. There were no significant differences in the total nitrogen, total alkalinity and pH between water of pond 1 and 2. In recent years, the chemical fertilizer has been used in cyprinus fish pond but many studies have showed that they were high risked as environmental pollution. Thus, the use of livestock manure especially cow dung could be a suitable alternative. The results showed the use of cow dung increased the bacterial population and diversity. Increasing of bacteria population caused increased total phosphorus that plays an important role in bloom of plankton because we found decreasing transparency and planktons are an important food for cyprinus fish. In despite of isolation of fish and human pathogenic bacteria from water, the bacteria growths were negative in all of the inoculated plate from fish liver and kidney. In summary, it seems the cow dung is a suitable alternative for fertilizing of cyprinus fish pond water if a microbial and physico-chemical monitoring of fish and pond water were continuously arranged.
    Keywords: Aquaculture ; Health ; Iran ; Pathogenic ; Bacteria ; Cyprinid ; Cow dung ; Fertilizer ; Microbial flora ; Cyprinus fish ; Dissolved oxygen ; Transparency ; Alkalinity ; Temperature ; pH ; Kidney ; Pseudomonas ; E.coli
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    Comparison of propagation efficiency of sobaity (Sparidentex hasta) fish rearing with fish capture from the sea in circular spawning tank (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25587 | 18721 | 2018-10-07 11:26:14 | 25587 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This project was conducted in imam Khomeini mariculture research state from October 2010 to June 2013 to compare reproductive performance between wild and domesticated Sobaity seabream broodstocks. In 3 years wild broodstocks did not spawn in 5 and 10 fiberglass and concrete tanks but domesticated broodstocks spontaneously spawned from February to March. In the first year domesticated broodstock in ratio of 3 females to 6 males allotted in 10 mt concrete tanks in triplicate. the about 2776000 eggs produced from 9 females and 18 males but further heavy mortality occurred because of winter shock syndrome and suppression of immunity system. Mean temperature, salinity and PH in incubation period was 16.5˚C (range between16-18˚C), 45ppt, 8.5 respectively. Mean floating eggs and hatch percent was 15.5% and 8.9% and about 19000 larvae produced. In the second year domesticated broodstock in ratio of 9 females to 23 males allotted in 5 mt fiberglass tanks in triplicate .about 17540000 eggs acquired from 27 female and 69 male broodstocks. Mean temperature, salinity and PH in culture period was 24.8 ˚C(range between20-28.3˚C), 47.5 ppt, 8.4 respectively. Mean floating eggs and hatch percent was 58.94% and 84% and about 8640000 larvae produced and stocking density was 10-30 larvae per liter. After 61 days mean production and survival were 503.3 (range between 140-1220) larvae per cubic meter and 5.3% (range between 0.47–12.2 %) respectively. Final mean weight of fry was 2.6 gr. In the third year domesticated broodstock in ratio of 3 females to 6 males allotted in 5 mt fiberglass tanks in triplicate. about 6570000 eggs acquired from 9 female and 18 male brood stocks. Mean temperature, salinity and pH in culture period was 23.9˚C(range between 19.5-28.4˚C), 45, 7.6 respectively. Mean floating eggs and hatch percent was 54.7% and 81.6% and about 2889800 larvae produced and stocking density was 10-30 larvae per liter. At the end of 87 and 89 days of husbandry the mean production and survival were 359 (range from 300 to 397) larvae per cubic meter and 2.7% (range between 1.32–3.8 %) respectively. Final mean weight of fry was 3.5 gram and Larvaeculture in the last 2 years carried out in 5 cubic meters fiberglass tanks. It seems to domesticated broodstocks because of spontaneous spawning, long spawning period, more brood stocks and possibility of reusing them in the next year are very economical for propagation than wild broodstocks.
    Keywords: Aquaculture ; Iran ; Sparidentex hasta ; Fry production ; Spawning ; Larvae culture ; Propagation ; Sobaity ; Sparidentex hasta ; Rearing ; Mariculture ; Broodstocks ; Temperature ; Salinity ; pH ; Survival
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    A survey on some risk factors and evaluation of their impacts on streptococcosis incidence in rainbow trout farms in Fars province (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25633 | 18721 | 2018-10-07 16:59:44 | 25633 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Streptococcosis is an infectious bacterial disease that causes huge economic losses in cold water aquaculture industry. Disease outbreak was experienced in some of provinces farms in recent years. Fars Province, has produced 7,000 tons of cold-water fish. According to Streptococcosis report in 1381 from the province and Proceedings have been performed during 10 years against disease and also economic losses impact of disease on rainbow trout production, risk assessment of Streptococcosis conducted on the plan. In this study, of 586 sick fish (have symptoms) studied 230 fish (39.24%) Streptococcus and (26.62%) gram negative bacteria were isolated. Of 754 healthy grower fish and fry (with no clinical signs) 10 fish or fry (1.32%)infected with streptococcus and 60 fish or fry(7.95%) infected with gram negative bacteria (Yersinia ruckeri, Pseudomonas, entrobacteriaceae). According to biochemical tests and molecular examinations, isolated Streptococcuus iniae and Streptococcus sp. Furthermore some physical and chemical parameters measured and aerobic bacteria of selected farm water counted. These factors effects on disease incidence and changes were evaluated by applying logistic regression.
    Keywords: Aquaculture ; Health ; Iran ; Fars province ; Streptococcosis ; Risk factors ; Rainbow trout ; Survey ; Diseases ; Fry ; Clinical signs ; Bacteria ; Yersinia ruckeri ; Pseudomonas ; Entrobacteriaceae
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    A survey on some risk factors and evaluation of their impacts on streptococcosis incidence in rainbow trout farms in west of Mazandaran province (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25632 | 18721 | 2018-10-08 07:24:27 | 25632 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This study aimed to investigate the effects of 5- parameter dissolved oxygen, PH, nitrite, nitrate and temperature of the water on Streptococcosis incidence in two groups of fry and grower fish. Research was performed at west Mazandaran province -Tonkabon region in 12 rainbow trout selected farms. Research conducted in 3 farms with well source water, 8 farms with water source of the river of Dohezar and one farm with water source of the river of Azarood, during 12 consecutive months. From 1390.04.01 to 1391.04.01, once time each month, and in each time 10 fish randomly sampled, inlet water were sampled simultaneously Of 1350 sampled fish 607 fish with an average weight 22.04 gr, average length 12.59 cm were in fry category and 743 fish with an average weight 156.25 gr, average length 23.32 cm in were grower category. Streptococcosis observed only in grower category Of 72 fish with clinical signs of the disease, 14 numbers were positive Streptococcosis (19.44%) and 58 numbers were negative Streptococcosis (80.55%). Three fish from grower category has not any clinical signs of disease and seemed to be healthy but were positive Streptococcosis in examinations (0.22% of total fish sampled. Fish with clinical signs of the disease but negative Streptococcosis were of at least 55.1 gr and at most weight 417 gr respectively. The results showed that 47.07% cases of Streptococcosis happened at 15.6°C water temperature, 35.29% at 16.98°C and 17.64% cases happened at 18.04°C so that 100% of Streptococcosis cases was observed at the average temperature of 16.99°C. In addition, the survey results show that despite relatively high levels of nitrite in source water of farms from wells, nitrite does not effect on the disease incidence. It seems that an optimal level of dissolved oxygen in water is effective in reducing the effectiveness of nitrite in this disease. According to equation coefficients logit model is as follows: According to Logit model, it seems that for every degree change in temperature and pH of water, morbidity change will diminish 0.37 % and 1.74 % respectively. 80.56% of fish sampled that had the clinical symptoms, was ranging from an average weight of 5.1 gr (fry) and 417 gr (grower fish), that despite having clinical signs of the disease were negative Streptococcosis. Isolation of Staphylococcus bacteria as well as Gram-negative bacilli from fish with clinical symptoms similar to the symptoms of Streptococcosis, may indicate the involvement of other pathogens in fish clinical signs.
    Keywords: Ecology ; Iran ; Mazandaran Province ; Tonkabon ; Streptococcusis ; Risk factors ; Cold water fish ; Survey ; Rainbow trout ; Dissolved oxygen ; pH ; Nitrite ; Clinical signs ; Temperature ; Pathogen
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    Quantitative evaluation of rainbow trout, Oncorhynchus mykiss cultured in brackishwater and freshwater of Yazd province, at the based on muscle (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25232 | 18721 | 2018-09-06 06:16:53 | 25232 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Fish culture has developed in Yazd province of Iran since recent decade and the rainbow trout, Oncorhynchus mykiss is the most important fisheries product in the area. There is a few information about carcass composition of cultured fish in Iran. Rainbow trout samples were collected from 6 fish brackish and fresh water fish farms of Yazd province during February 2007. Fish muscle samples were freezed in -30 C after being homogenized, till laboratory analyses. Proximate composition of samples contained 17.05-18.53% crude protein, 2.35-5.13% total fat, 1.31-1.7% ash and 74.18-77.05% wet. Saturated fatty acids including palmitic acid (14.06-19.60%), stearic acid (6.44-6.1%), myristic acid (1.29-5.66%) and lauric acid (0.13-2.76), also unsaturated fatty acids oleic (30.24-40.14%) and linoleic (21.18-38.85%) were extracted from muscle tissue of fish of different farms, using GC. Other unsaturated fatty acids including -linolenic acid, EPA and DHA had low concentrations (1-3%) in samples. Vitamin E level was 4.33-94.34 g/100gr. Concentration of minerals in homogenated muscle tissues were as: Na 822.58-1052.78ppm, K 12272.75-13715.25ppm, Ca 104.75-171.03ppm, Fe 19-40ppm, Cu 0.044- 1.01ppb, Zn 7.02-12.47ppb, Cd 0.879-1.099ppb and Cr 1.895-3.86ppb. Heavy metal concentrations were lower than permitted values.
    Keywords: Aquaculture ; Iran ; Yazd province ; Oncorhynchus mykiss ; Rainbow trout ; Fish culture ; Tissues ; Brackish water ; Muscle
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    The study of establishment of rainbow trout culture and hatchery farms on Zayanderood’s River region in Caharmahal and Bakhtiyari Province (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25620 | 18721 | 2018-10-14 02:36:27 | 25620 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The aim of this study was to find the best location for establishment of rainbow trout culture and hatcheries farms on Zayanderood’s river region in Charmahal and Bakhtiyari province. This survey carried out over ten station along Zayanderood’s river. The result of physical and chemical analysis showd that the annual average of air temperature varied from 9.5oC - 10oC where the pH annual average value were between 7.5 and 8.8. the dissolve oxygen concentration in stations except in rainbow trout farm effluent were above 10 mg/l. the other chemical , pollutant as well as pesticides levels were under the limiting concentration for rainbow trout culture and Hatcheries activity. The plankton survey showed that the Bacillurophyta were the dominant group of phytoplankton where protozoa constituted the most abundant group of zooplankton the Benthic organisms sensitive to pollutant in particular Epirus were dominant group in all stations. In regard to fishes presence in river, five species of Ciprinidae, one species from Balitoridae and one species from salmonidae families were identified. The capacity for development of rainbow trout culture for tow phase period in Zayanderood’s river region with respect to self purification potential (self purification potential were determined from the oxidation of the effluent of the only active trout farm of the river region), minimum of 10 L/s water requirement for production of trout in concrete canal and pond system and 1 L/S water need for production in semi circular closed system were estimated to be 5202 metric tons.
    Keywords: Aquaculture ; Iran ; Caharmahal and Bakhtiyari province ; Positioning ; Rainbow trout ; Self purification ; Culture ; Hatchery ; Survey ; Temperature ; pH ; Dissolved oxygen ; Pollutant ; Plankton ; Bacillurophyta ; Phytoplankton ; Protozoa ; Zooplankton ; Benthic organisms ; Ciprinidae ; Balitoridae ; Salmonidae
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    The identification of best location for rehabilitation of coral stock (Acropora spp.) in the Larak Island (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25637 | 18721 | 2018-10-08 08:10:22 | 25637 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Nutrients, organic matter and benthos communities of sea bed, health and growth of Staghorn corals (Acropora spp.) were investigated at two stations on the Lark Island from Jul.2012 to Feb.2013 seasonally. ANOVA test was used for the spatial and temporal variations in the studied between stations and seasons (95 percent confidence interval). Also, Principal Component Analysis (PCA) factor was used in order to identify the most important parameters influencing of coral growth. ANOVA test indicated difference between Biometrics new coral colonies at both the station and the old coral colonies in the West Island Station Lark in the summer and spring together with autumn and winter(P〈0.05), Although there was no significant difference between autumn and winter(P〉0.05). Salinity factor was similarity (P〉0.05), but the factor of temperature was difference in the summer with other at both stations in all seasons (p〈0.05). Diving observations indicated that adaptations of in the coral habitat at both stations is selected. In order to avoid of stress caused by fluctuations in temperature and salinity at both stations, Staghorn corals were distributed in the region away from the coastline. Also, these communities have chosen transverse expansion strategy a way to escape the stress of sea surface temperature fluctuations. Significant correlation was reported between temperature fluctuations and the development of the Lark island's coral bleaching. It also has been reported to the Persian Gulf corals are vulnerable to unusual changes in temperature and long term stress. Studied nutrients (nitrate, nitrite, phosphate and silicate) in front of Lark station had a higher amount of station of West Island. The results of nutrients (nitrate, nitrite, phosphate and silicate) did not show any difference in the previous studies in the larak coral ecosystems. Therefore, the probability of coral loses in front of Larak station, caused no recycling of materials, and increased accumulation of nutrients in the current situation. Benthos communities of bottom were differed (P〈0.05), but the density of these communities, there were no differed in the two stations in the same season (P〉0.05).The study of Gran size of seabed was showed the highest amount of sand in all seasons at both stations. Studies showed that, in the absence of environmental stress, the type of bottom sediments had as an important factor in the distribution and abundance of benthic organisms. Although coral life is not directly related to the richness of the seabed, but production in marine ecosystems directly and indirectly related to these communities, which is influential on the richness of these ecosystems. In studying Coral Watch method were concluded difference between indices used in seasons of summer and winter together, and with autumn and winter seasons (p〈0.05), although no differences was observed between autumn and winter(p〉0.05).The indices obtained in this study can be concluded the minimum stress during the period of the study was dominated ecosystems, also difference between summer and spring of this study could have been influenced by the effect of temperature on zooxanthella concentration. Two components were identified in this study using of principal component factor analysis to identification the most important parameters that influence the growth of corals. Also, the variability of each factor studied was different.Highest loading factor on the first component of turbidity, temperature and salinity, and the second component loading factor maximum nitrate, nitrite and phosphate are inversely associated with coral growth. According to consideration the ecological conditions were determined two area to rehabilitation stocks of Staghorn corals around the Lark Island by using ArcGis software.
    Keywords: Aquaculture ; Iran ; Persian Gulf ; Larak Island ; Rehabilitation ; Coral stock ; Acropora spp. ; Nutrients ; Organic matter ; Benthose ; ANOVA ; Growth ; Temperature ; Zooxanthellae
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    The use of lactic acid bacteria metabolites (nisin and organic acids) for the shelf life extension of trout (Oncorhynchus mykiss) and their effect on dominant bacteria in inoculation study (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25650 | 18721 | 2018-10-14 02:41:46 | 25650 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Nisin is a natural antimicrobial and has inhibitory effect on the pathogens and spoilage organisms. The efficiency of nisin reduced after reaction with food compounds. There is the hypothesis that combination of nisin with organic salt increases the shelf life of fish. The purpose of this study was mainly to evaluate the effects of nisin (0.15 %) and sodium acetate ( 1% ) individually and in combination on shelf life of fillets and whole trout ( without viscera). then, the behavior of Listeria monocytogenes and Clostridium botulinum type E were evaluated in different time at 40C. Physical and chemical parameters including: pH, Peroxide Value (PV), Tiobarbithoric Acid (TBA) and Total Volatile Nitrogen (TVN) and bacterial factors such as Total Viable Count (TVC), Pscycrothophic Count (PTC) and Lactic Acid Bacteria (LAB) were examined. These parameters were done at intervals of 4 days for 16 days. Results showed that chemical and bacterial factors of whole trout (Oncorhynchus mykiss) (without viscera) have been more favorable than the fillets . It also showed that the peroxide value, the thiobarbituric acid, volatile nitrogen bases, and pH in combination of sodium acetate and nisin Z treatments were exposed to significantly compared to control treatments (no preservatives) (P〈0.05). Treatments with sodium acetate and nisin Z (individual) were exist after combination treatment. Hence it can be concluded that the maximum shelf life of in preservative treatments was 16 days, but control treatment was 12 days in some cases and in some cases 16 days. Effects of treatments on Listeria monocytogenes and Clostridium botulinum in the control treatment showed the highest growth was observed in both species (log 8) but in single and combined treatment of bacterial growth slower , but is still the trend the 16th day of so Listeria and Clostridium log 5 to log 6, respectively. Vegetative cells of Clostridium were more sensitive in compare to Listeria. When using preservatives (chemical or biological) in fish tissue, parameters such as the type of preservative, used method, pH, proteolytic enzymes, and the incidence of resistant strains and so on influence of antimicrobial agents used. The overall conclusion of the study was shelf life of whole trout (without viscera) was more than fillets, combination of nisin and sodium acetate were better than other treatment and finally higher doses of bilogival preservatives are need to for achieve to significant reduction of pathogenic bacteria.
    Keywords: Fisheries ; Iran ; Nisin Z ; Sodium acetate ; Rainbow trout ; Listeria ; Monocytogenes ; Clostridium botulinum ; Lactic Acid ; Bacteria ; Organic acids ; Oncorhynchus mykiss ; Pathogen
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    Epidemiological study on some environmental and management parameters affecting on WSD occurrence in Fenneropenaeus indicus and Penaeus vannamei (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25652 | 18721 | 2018-10-08 08:43:54 | 25652 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: For the first time white spot disease (WSD) was reported in shrimp farms of khoozestan province, in southwest of IRAN in 2002. Then in 2005 the neighbor province, boushehr, was contaminated. In 2008 WSD outbreak reported in sistan-bloochestan province in southeast of Iran. In 2015 all of southern shrimp farms of country except Hormozgan, the middle southern province, which has remained free of WSD, are being contaminated. White Spot disease suspended shrimp culture in thousands hectares of shrimp farms. Considering that white spot disease has not been observed in Hormozgan province yet, the question is; to what extent environmental and management factors participated in preventing WSD outbreak or cause WSD outbreak. In this study (20102012), the effects of environmental factors and management, stressors that decrease immune system function of shrimp are discussed. In addition, the role of pathogen as the main factor of outbreak is discussed. The goal of this study is to define environmental parameters and management practices associates with outbreak of white spot disease in affected provinces and discover reasons of being Hormozgan province free of this disease. In this study the role of the local environmental factors and management practice stressors in susceptibility to WSD was determine. Both the effects of environmental factors in water of ponds including total ammonia, nitrogen, dissolved oxygen, pH, salinity, transparency, and temperature and management issues related to biosecurity are studied. There were overlaps on physical and chemical parameter values obtained in clear areas with contaminated areas .Results of the data analysis suggest that lack of association with WSD incidence was 7 times greater than WSD incidence despite of disease outbreak in sistan-bloochestan province, so other sources of white spot disease virus incidence was suspected in affected areas. Histopathological examinations and polymerase chain reaction (PCR) tests during project performance did not reveal white spot disease virus evidences in post larvae examined from khoozestan province stocked in farms but disease outbreak was happened in that farms , so we suspected to management practice include feed , pond preparation and carrier of disease. Recorded values of temperature and salinity in some months during inspection in Hormozgan province specified stressful condition that may lead to WSD outbreak, however the disease did not appear. Therefore the hypothesis that the water physical and chemical conditions are reasons to prevent disease outbreak in Hormozgan province is being rejected. The policy of Hormozgan’s fishery authorities, to replaced Fenneropenaeus indicus with specific pathogen free Litopenaeus vannamei, that is more resistant to some of diseases, before incidence of WSD in farms and to before being endemic in the Hormozgan province, made an advantage compare to affected southern provinces that introduced Litopenaeus vannamei after WSD prevalence to their farms. However it does not guarantee to maintain current trend of being Hormozgan province farms free of white spot disease. Therefore establishing the principals of biosecurity are strongly emphasized. Strategies taken by the proficient authorities in preparation of SPF shrimp broodstock can be the most important factor in preventing WSD. Regarding biosecurity principals purchased feed must be free of shrimp head powder. Construction the new shrimp farms should be as far as it could be away from contaminated areas.
    Keywords: Aquaculture ; Ecology ; Health ; Iran ; Khouzestan Province ; Boushehr province ; Sistan and Balochestan province ; Hormozgan Province ; Epidemiological study ; WSD ; Fenneropenaeus indicus ; Penaeus vannamei ; Shrimp ; White Spot Disease ; Temperature ; Salinity ; PCR ; Polymerase Chain Reaction ; Ammonia ; Nitrogen ; Dissolved oxygen ; pH ; Transparency
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    Monitoring on nutrients of sediment with emphasis on algal bloom event in the southern of Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25657 | 18721 | 2018-10-08 08:23:30 | 25657 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This study was conducted to spatio-temporal fluctuation and distribution of phosphorous and nitrogen species, total organic carbone and some environmental parameters at suface sediments in the southern of Caspian Sea. In current study, 48 samples were collected from four stations (Bandar Anzali, Tonekabon, Bandar Nowshahr and Bandar Amirabad) and three dephths (5, 10 and 20 meter) during four seasons in 2013-2014. Results of this study showed that annual mean of Adsorbed-P, Fe-P, Al-P, Bioava.-P, Ca-P, TIP, Rese.-P and TP were obtained 6.68±0.63, 60.90±2.44, 24.94±2.39, 94.52±3.62, 1868±36, 1963±37, 87.51±9.52 and 2050±38 µg/g.dw, respectively. Result also showed that inorganic phophotous was maximum during different seasons, however, percent mean of residue-P which containe organic compounds and non-degrediable compounds, was less that 5 percent. Percent of Ca-P was higher than 90, whereas Bioava.-P was less that 10 percent. The order of different forms of phosphorous were registered Ca-P〉Org-P〉Fe-P〉Al-P〉Adsorbed-P.Annual mean of NH4/N, NO3/N, TIN, TON and TN were observed 2.56±0.27, 0.89±0.12, 3.52±0.26, 6.06±0.27 and 9.57±0.32µg/g.dw respectively. Annual percent of TON was two folds than TIN and NH4/N was also three times than NO3/N. In addition, mean of Fe, Al, Ca, Eh and TOC% were obtained 25.39±0.65, 18.76±0.68, 60.15±1.50 mg/g.dw and 67.02±0.68, 1.11±0.09, respectively. Maximum mean of carbon (1152±171), nitrogen (0.87±0.05) and phoporous (74±23) µM/g were observed in fall, winter and summer, recpectively. Also, maximum ratios of TOC:TP (16.8±2.3) and TOC:TN (2378±363) were obtained in fall season. As a conclusion, mian role of Adsorbed-P, Fe-P and Al-P adsorbed and desorbed were temperature, Eh and pH, respectively. Results of this study showed that TOC:TN was very high which represented to excitance of organic matter with almost non-degrediable. Whereas, TOC:TP was low which showed that organic phophoros was not related to proteins and phospholipids therefore sources of org-P were belong to other compounds.
    Keywords: Ecology ; Iran ; Caspian Sea ; Anzali ; Tonekabon ; Nowshahr ; Phophorous ; Carbon ; Nitrogen ; Environmental parameters ; Surface sediments ; Nutrients ; Algal bloom ; Species ; Samples ; Temperature ; pH ; Proteins ; Phospholipid
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    The study of pollution in the Gorgan bay (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25679 | 18721 | 2018-10-08 09:40:22 | 25679 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: From an environmental standpoint bays around the world are very important ecosystems including the Gulf of Gorgan Bay and thus are important. Which has a relatively low depth, a little flow, temperature appropriate, biogenic materials and abundant food in the sea suitable for children sometimes place (Nursery ground) and also the perfect place for power (Fattening ground) is an early sea fish. In this research effort will be the initial studies on the impact of environmental pollutants on the ecosystem of the Gulf of Gorgan and the quality and quantity of pollution, especially pollution, agricultural pesticides and entering the Gulf of Gorgan and the impact on the ecosystem's the initial assessment and the monitoring of changes in them over the years. The user in the Gulf of Gorgan, agriculture. Pollution from agricultural fields related to pesticides and fertilizers are used in the fields. According to consumer research, and the remaining amount of pesticides in water and sediments of the Gulf of measured, indicated that most of the remains of chlorinated pesticides in sediments of the pesticides used in the agricultural sector In addition, all the toxins in the summer and spring peak. In addition, the DDE concentration compared to other poisons. In the case of other contaminants in the area is now threatened by the Gulf of Gorgan, although not serious, but it seems that the increase in organic matter and reduce the amount of oxygen of the water. The near future to Eutrofication risks in the ecosystem.
    Keywords: Pollution ; Iran ; Gorgan Bay ; Pollution ; Ecosystem ; Temperature
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    Survey on physicochemical parameters and pollutants in artificial reefs in Hormozgan province (Bandar-e- Lengeh area) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25265 | 18721 | 2018-09-07 07:24:14 | 25265 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Survey on physicochemical parameters and pollutants in artificial reefs in Hormozgan province (Bandar-e-Lengeh area) was conducted during 2004-2007. After establishing of artificial reefs by Hormozgan Shilat office, selection of sampling stations is performed at summer and winter seasons. Summer and winter values for temperature were 32.9 0.5 and 21.4 0.5 centigrade. pH had a little range of tolerance (8-8.95) and its annual mean was 8.81 0.13 . Transparency is an effective parameter on monitoring of artificial reefs. Summer and winter values for Transparency were 3.8 3.72 and 5.63 0.78 meters. Annual mean of dissolved oxygen and salinity were 7.11 0.1 ppm and 39.13 0.26 ppt. Annual means of dissolved nitrate, nitrite and orthophosphate were 4.9 0.7, 1.44 0.09 and 0.97 0.06 micromole per liters respectively. Measurement of chl.a was done in summer only. The maximum concentration was 2.95 microgram per liter. Annual average percentage of silt, clay and sand were 33 10.68, 21.63 4.84 & 45.96 12.66 respectively. Organic Carbon percentage had a little rang (0.61 1.92) of changes. Its annual percentage was 0.99 0.2. Summer and winter values for COD were 1.53 0.48 & 1.62 0.48 milligrams per liter. Concentration of heavy metals (Cu, Zn, Fe, Ni & Pb) in sediments were determined .All of heavy metals (especially Pb) had higher concentration in summer than winter. The annual means of Cu, Zn, Fe, and Ni & Pb were 26.5, 54.3, 27.6, 70.85 & 48.15 micrograms per gram of dried sediment. Normal aliphatic hydrocarbons (C10-C 30) were determined in water & sediment. Annual average and the summer and winter values for normal aliphatic hydrocarbons in water were 258.7, 217.8 & 299.6 micrograms per liter and for sediment were 5726.8, 12616.8 & 2132.2 micrograms per gram of dried sediment.
    Keywords: Ecology ; Pollution ; Iran ; Persian Gulf ; Hormozgan Province ; Bandar-e-Lengeh ; Artificial reef ; Heavy metals ; Aliphatic normal hydrocarbons ; Physicochemical parameters ; Survey ; Pollutants ; Sampling ; Temperature ; pH ; Transparency ; Nitrate ; Nitrite ; Sediments
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    Monitoring infectious agents in L.vannamei farms in choebdeh-Abadan (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25262 | 18721 | 2018-09-07 07:28:07 | 25262 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The introduction of L. vannamei to Iran from Hawaii was initiated when high mortality occurred in shrimp farms of Abadan in 2002 than in Bushehr during summer of 2004. Inspection of L. vannamei for infectious agents (Bacteria , virus , fungi and parasite ) , determine total plate count and total vibrio in Bahmanshir Rivers, shrimps and farms water , examination physicochemical factors of farms water , Identification of carriers of wssv in wild shrimp and crabs , the main objectives of this study , which was held in 2007 . 540 shrimp samples (pl12 pl15) and subadults were collected from different shrimp farms in khouzestan province and then check for identity bacteria, fungi and parasite. Also 280 samples of L. vannamei , wild shrimps ( Metapenaeus af inis , Exopalaemon styliferus ) and crabs (Grapsus sp .and Sesarma sp. ) were Collected from Bahmanshir river for virology studies by PCR procedure (Iq2000 kit WSSV , IHHNV , TSV ) . 120 samples of L. vannamei for histopathology had been collected randomly and preserved in Davidson’s fixation and then transferred to 75% ethyl alcohol for storage. (Hepatopancreas gills). Physicochemical parameters of water in culture ponds comprising of pH DO, BOD, NO_3, NO_2, salinity. Total. Hardness and NH3 were measured all over culture period for 340 times. Finally it has been detected 10 genus and species of bacteria consisting of V . alginolyticus, V. parahaemolyticus, V. proteolyticus , plesiomonas shigelloides ; 6 genus and species of fungi specially Aspergilus niger , Asp.fumigatus and Asp.flavus and two genus of parasites vorticella sp. And Zoothamnium sp. were isolated. Crab (Sesarma sp.) were tested by using Iq2000 diagnostic kit for WSSV that positive for WSSV. There was a positive result (three viruses TSV, IHHNV and WSSV) for postlarvae and subadults of L.vannamei by using Iq2000 kit and IFRO kit (Internal kit). Histopathological studies have shown inclusion bodies of TSV, WSSV, IHHNV, MBV and HPV in various tissues. Results demonstrated the mean of vibrio count (0. 01×103 - 1.96 ×103) and tolal plate count (0 .21 ×103 - 14.25 ×10 3). physicochemical parameters of water were measured as follows : salinity (12.3 22.5 ppt ).BOD5 ( 1.98 10.21 ppm ) , DO (3.17 11.25 ppm ) , NH3(0.02 3.45 ppm ) and temperature (20 31. 5 c) . Keywords: L.vannamei, bacteria, virus, fungi, parasite Histo pathology - physicochemical parameters of water- Abadan khouzestan province.
    Keywords: Aquaculture ; Iran ; Khouzestan Province ; Abadan ; Choebdeh ; Bahmanshir River ; L.Vannamei ; Bacteria ; Virus ; Fungi ; Parasite ; Histopathology ; Physicochemical parameters of water ; Mortality ; Shrimp ; Metapenaeus affinis ; Exopalaemon styliferus ; Grapsus sp. ; Sesarma sp. ; Aspergilus niger ; Asp. Fumigatus ; Asp. Flavus ; Temperature
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    Utility improvement of brackish water cultural earth ponds of rainbow trout (Oncorhynchus mykiss) through establishing enclosure medium (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25299 | 18721 | 2018-09-07 08:25:10 | 25299 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Considering increase in production of earth ponds in brackish water condition, this research carried out at Bafgh fisheries research station to improve efficiency and production of brackish water cultural earth ponds of rainbow trout (Oncorhynchus mykiss). This study was performed in four 0.5ha earth ponds in two treatments with two replications. Each treatment included two earth ponds so that fish farmed in two culture mediums consisting open medium and %20 enclosure medium of pond (pen medium).for this purpose 20% of earth pond s space separated via a fixed net wall with 15mm in mesh. In this research some main growth equations and economical factors were studied. Data were analyzed using SPSS software, Pierson, Toki, Dankan, Dant analysis and also descriptive statistics. The significant differences were determined in %5 level. Since there was a connection between pen medium with free one, no signs based upon disturbance observed in measured environmental factors in this space. Statistical results indicated a significant increase in %WG, %FE, % SGR, %CF and final biomass in pen medium treatment(p〉0.05). According to economic results, this treatment also differed significantly from open medium treatment about ratio of marketable size production and net income so that yield quantity and production income increased. Therefore, through pen culture in 0.5 ha earth ponds and likely larger ones, utility and production of farm improve and can be a good approach for farmers in order to better exploitation of possibilities and facilities.
    Keywords: Aquaculture ; Iran ; Bafgh ; Surrounding medium ; Earth pond ; Rainbow trout ; Utility ; Brackish water ; Culture ; Oncorhynchus mykiss ; Growth ; Yield
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    Breeding and reproduction of grey mullet (Mugil cephalus L.) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25297 | 18721 | 2018-09-07 08:04:10 | 25297 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Mazandaran and Gorgan provinces have temperate climate, thus they have more potential for aquatic animal culture. There are thousands hectare of salt and useless lands in adjacent to Caspian Sea. As these areas have provided a favorable back ground for aquatic animal culture. As a result, the successful results obtained from imported gray mullet (Mugil cephalus) culture project in north climate, it has demonstrated that the gray mullet has a good biocharacteristic for culturing in pond enclosure environment and in different aquatic conditions (fresh water, brackish water and salt water). From 1998 until 2001, the broodstock yield and gray mullet artificial propagation projects were performed by fisheries research center of Mazandaran in Ghomishan prawn culture station in adjacent to Caspian Sea (East north of Mazandaran Province). This investigation executed during two stages (phases). At first stage, the goal of this project included the survey of possibility available about matured fish as well as induction of final maturation and artificial propagation for producing of broodstock and larva. In addition, in this way, we will obtain new information about gray mullet propagation and culture as marine species. We introduce mass production in aquaculture. At present research, the possibility of broodstock yield and artificial propagation of gray mullet have investigated by gray mullet fingerlings imported from Hong kong and then they have cultured in earth ponds of Ghomishan areas during five years (1994-1998). In order to broodstock production in spring 1998, two earth ponds (0.5 hectare) were prepared. 100 specimens of fish stock (1-2.5 kg weight average and 5 years of age) placed in each pond. For providing of suitable water and nutrition, fish were fed by food containing rich protein (40%) with 3-5% body weight and maintained in water with 30-35 ppt salinity. The survey of sexual maturation was performed by sampling of sexual glands through year. There were four stages in dominant female broodstocks. This survey indicated that oocytes have emerged stage 1 (immature) from March to June, stage 2 (yolk vesicle) in September and stage 4 (yolk globule) in October. Three stages (first, second and third) of yolk formation in oocytes will occur but these stages take place in October, December and January respectively. It's obvious that oocytes will progress into the end of third stage (yolk formation) and then their growth was arrested. Ovum with 600 m diameter was observed when the water temperature declined less than 18 c and day time was short (from middle autumn to middle winter), on that time, fish were induced by hormone because lack of final maturation and ovulation, therefore, natural spawning was not occurred in pond condition. Furthermore, artificial propagation of mugil cephalus was occurred by hormonal induction. Hormonal induction was utilized by inject of many hormones (LHRH, Cph, HCG). Using different components of hormones and also their different doses obtained the best results from broodstock that the average ovum diameter was about 600 m. Several types hormones which were injected into gray mullet with two or several intermittent (24 h intervals) along with 20-25 c temperature and 30-35 ppt salinity. This condition can provide stage 4 maturity for fertilization. Totally (as whole), three intermittent fertilization was necessary for exiting of larva, larva production in first, second and third intermittents that were 2000, 2500 and 300 specimens respectively. Larva fed on chlorella algae and rotifera and they have maintained for 14-15 days. The results of obtainable research indicated the possibility of gray mullet broodstock production in cultural condition, artificial propagation and larva production. This research took place for the first time in north climate of Iran.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Mazandaran Province ; Gorgan ; Ghomishan ; Gray mullet ; Mugil cephalus ; Broodstock ; Production ; Yield ; Artificial propagation ; Hormone ; Temperature ; Culture ; Maturation ; Fingerlings ; Earthen ponds ; Ovum ; Spawning ; Breeding ; Reproduction
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    Survey the effects of live rainbow trout handling on fish meat quality (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25321 | 18721 | 2018-09-08 16:49:00 | 25321 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This research was performed to survey the live handling condition of Rainbow trout in Tehran province and determine the effects of this method on the quality of the flesh regarding with water binding capacity, pH changes, moisture, fat and protein content in different seasons of year. Based on reviews of Questionnaires, there were 20 main live rainbow trout sale centers in Tehran. 10 numbers of these were in fruit and vegetable centers of municipality and others were out of these centers. Live fish handling to in these areas mainly was performed from fish farms located in the Haraz road (85 percent), farms of Firoozkooh road (10 percent) and others were (5 percent) from neighboring provinces. The results of this study indicated that current methods of live handling in Tehran (the transporting time 4 h and the density of fish in tank 100-150 kg /m^2) meat quality of transported trout was not affected. Water holding capacity of transported fish was less than caught fish in the fields. This amount was reached to lowest level in in summer as well as the differences between the two groups were significant (P〈0.05). Also, when a meat pH level was elevated, water holding capacity was reduced. In summer, the amount of WHC in transported fish was 13.4% that was a highest value between the seasons. Appears that in the summer, high temperature and increasing kinds of stresses to fish during handling due to excessive accumulation of metabolites such as ammonia, muscle energy sources emptied because of high activity. In this condition, falling of pH during postmortem changes being moderated and lead to increase drip loss in the transported sample.
    Keywords: Aquaculture ; Fisheries ; Iran ; Tehran province ; Haraz ; Firoozkooh ; Live Handling ; Rainbow trout ; Meat quality ; Seasons ; pH ; Moisture ; Fat ; Protein ; Temperature ; Sample
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    Study of anaesthetic effects of essence, water and water-alcohol extractions of clove oil (Eugina caryophillata) in some cultured species of aquatic animals (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25113 | 18721 | 2018-08-23 06:07:06 | 25113 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Anaesthetic effects of essence, water and water-alcohol extractions of clove oil were studied in rainbow trout (Oncorhynchus mykiss), common carp (Cyprinus carpio), Persian sturgeon (Acipenser persicus) and Penaeus semisulcatus under various water quality conditions consisting of water temperature, pH and salinity. The obtained results showed that both water and water alcohol extractions had no anaesthetic effect at concentration upto 2000 mg/L whilst, essence could aet as a suitable anaesthesia at 〉50 mg/L. The most suitable anaesthic effect by essence in rainbow trout was 250 mg/L provided at 5 C and pH 7. Also, clove oil essence showed the best anaesthetic effect in carp under water quality conditions consiting of water temperature at 25 C and pH 7-8 or water temperature at 30 C and pH 9. In addition, clove oil essence at 75 mg/l and 12 mg/L gave the best anaesthetic and sedation effects in Persian sturgeon provided at 21-23 C and pH 8.3, respectively. Furthermore, clove oil essence at concentrations above 50 mg/L was able to act as an anaesthesia in P. semisulcatus. However, the best anaesthetic concentration was depended on water quality conditions consiting of water temperature, pH and salinity.
    Keywords: Biology ; Iran ; Clove oil ; Rainbow trout ; Common crap ; Persian sturgeon ; Penaeous semisulcatu ; Water quality ; Oncorhynchus mykiss ; Acipenser persicus ; Cyprinus carpio
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    The effect of thyroxine (T4) on prematurating in female, on survival percent, growth and development of egg and larval in rainbow trout, grass carp, silver carp and barbell of Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25118 | 18721 | 2018-08-22 02:17:23 | 25118 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Studies show that thyroxine can play an important role in regulating growth and other physiology activities. Since the direct role of thyroxine in growth metamorphose larval of bony fishes hasn't been proved yet, during the experiment accomplished in Shahid Ansari and Jajrood station, the hormones function in the survival of development of silver carp, grass carp and rainbow trout were studied. In this study accomplished of thyroxine baths with 0.1, 0.5 and 1 ppm (with 3 repeat). The other phase, thyroxine injected to females grass carp, silver carp, rainbow trout and barbell of Caspian Sea with different doses (1, 10 and 100 g/g B.W.). Tehn survival of development of embryo and larval and precent of fertilization were studied. Also, the percent of mortality were compared in two phases in stages of development. Results show that: 1) Trout: a) Phase of thyroxine bath: The number of hatching eggs and survival of larval in 0.5 ppm were increased to other treatment. b) Phase of hormone injection: The survival of larval in treatment of 10 g/g was 8.58% that was meaningful difference to other treatment (P〈0.05). 2) Silver carp: a) Phase of thyroxine bath: The number of hatching eggs and survival of larval in 0.5 ppm were (20%) increased to other treatment (P〈0.5). b) Phase of hormone injection: The survival of larval in treatment of 10 g/g was meaningful difference to other treatment (P〈0.05). 3) Grass carp: a) Phase of thyroxine bath: The number of hatching eggs and survival of larval in 0.5 ppm were 39% increased to other treatment. b) Phase of hormone injection: The survival of larval in treatment of 1 g/g was meaningful difference to other treatment (P〈0.05). 4) Barbel of Caspian Sea: Development stages wasn't determined in this fish. Number of eggs degenerated, number of eggs were the first development stages and growth of numbers increased on ovary.
    Keywords: Biology ; Chemistry ; Iran ; Caspian Sea ; Thyroxine ; Female ; Survival ; Growth ; Egg ; Larval ; Grass Carp ; Silver Carp ; Thyroxine (T4)
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    Effects of different levels of folic acid and vitamin C in rainbow trout (Oncorhynchus mykiss) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25332 | 18721 | 2018-09-13 12:55:23 | 25332 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Juvenile rainbow trout, Oncorhynchus mykiss (initial body weight 14 ± 0.25 g) were fed diets [crude protein 420 g/kg; gross energy 16.8 MJ/kg dry matter; crude fat 110 g/kg] containing deficient, adequate or excessive amounts of vitamin C and folic acid to investigate the potential nutritional effects on growth factors. A growth trial was conducted over 9 wks at a water temperature of 12 ± 1 °C. Four semipurified diets were supplemented with 0, 30, 60 and 90 mg /kg vitamin C and 0, 1, 2 and 3 mg/kg folic acid and were fed to fish in triplicate tank for 9 wks. At the end of the growth trial, the percent of survival, final body weight, weight gain, feed intake, feed conversion ratio, protein efficiency ratio and specific growth rate were measured. The results that growth factors were significantly (P〈0.05) affected by dietary vitamin levels. Inclusion levels of 2 mg/kg dietary folic acid and 60 mg/kg dietary vitamin C is acceptable, for use in rainbow trout diets, under favorable economic conditions. This study shows that increasing dietary folic acid and vitamin C could improve growth performance in juvenile rainbow trout.
    Keywords: Aquaculture ; Iran ; Nutrition ; Folic acid ; Vitamin C ; Rainbow trout ; Oncorhynchus mykiss ; Juvenile ; Growth ; Survival
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    Study of water's physico-chemical characteristics in the southern Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25337 | 18721 | 2018-09-13 12:43:04 | 25337 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This study was conducted to determine the physico-chemical characteristics of water during four season and 8 transects (Astara, Anzali, Sefidroud, Tonekabon, Nowhshar, Babolsar, Amirabad, Bandar Tourkman) in the Southern of Caspian Sea in 2009-2010. 480 samples were collected at different water layers and then physicochemical parameters were measured based on standard methods. Result of this study showed that surface water temperature was varied from 7.2 to 29.8◦C in winter and summer, respectively. Minimum fluctuation of water temperature was observed at 100 m depth (6.8-10.3◦C). Mean value of water transparency was obtained 4.91±0.24 m. This value increased from inshore to offshore. pH value was fluctuated from 7.15 to 8.83 unit with variance of 1.54. Maximum DO concentration was observed at surface layer (8.40±0.08 mg/l) with 137±18 saturation and minimum was at 100 m depth (6.46±0.18) with 86.1±2.8 saturation. The nitrite, nitrate and ammonium concentration were ranged 0.0-0.2, 0.0-4.6 and 0.05-7.12 µM, respectively. Maximum value of TN was observed at inshore and minimum at offshore (100m). Nitrite concentration decreased from inshore to offshore but increased from surface to the bottom (100 m). The inorganic phosphorous increased at surface water and also at the bottom. Trend of inorganic and TP was similar. Minimum of N/P ratio were observed at summer (5.48±0.38) and maximum value at winter (9.13±0.46). This value of N/P ratio showed that the growth of phytoplankton was limited by nitrogen. The dissolved silicate was decreased from spring (230.7±6.65 µg/l) to winter.
    Keywords: Ecology ; Iran ; Caspian Sea ; Astara ; Anzali ; Tonekabon ; Nowhshar ; Babolsar ; Bandar Tourkman ; Sefiroud River ; Water ; Physico-chemical parameters ; Temperature ; pH ; Phosphorous ; Phytoplankton ; Nitrogen ; Dissolved silicate ; Fluctuation
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    Hydrology, hydrobiology and environmental pollution in the southern of Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25325 | 18721 | 2018-09-12 10:04:00 | 25325 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The phytoplankton, zooplankton, Macrobenthos and ctenophore Mnemiopsis leidyi with Physico-chemical parameters and pollutants of water were investigated in the Caspian Sea during 2007-2008. The sample station located between 48° to 54° longitude and 36° to 39° latitude. The samples were gathered in four seasons and eight transects (half-line perpendicular to the coast) in Astara, Anzali, Sefidrud, Tonekabon, Noshahr, Babolsar, Amirabad and Turkmen. Sampling was done of 5, 10, 20, 50 and 100M depth of different layers. Nonparametric test and multivariate analysis (PCA) were used for statistical methods. Physico-chemical factors: The results showed that the changes of physico-chemical factors, includes: water temperature, salinity, organic nitrogen and inorganic nitrogen (ammonium) are most important than other factors. The lowest sea surface water temperature was recorded in winter (7.2°C) and highest in summer (29.8 °C) (p〈0.05). However, there was not significant difference at 50 and 100m depths in different seasons (p〉0.05). The lowest salinity was recorded in winter (10.42±0.14ppt) (p〈0.05) and the rest of the seasons, there was not significant difference (p〉0.05). The most inorganic nitrogen was determined (ammonium ion: 40.8±2 µg.l^-1) in winter and the highest organic nitrogen (660.6±92 µg.l^-1) in summer (p〈0.05). The concentration of inorganic nitrogen was evident from the surface layer to a depth of 100 meters (p〈0.05), but this trend was not observed in inorganic nitrogen (p〉0.05).The maximum concentrations of total nitrogen were measured at the near-shore stations (640.6±28 µg.l^-1). The annual average water transparency was 4.91±0.24 m and increased in away from the beach (p〈0.05). Phytoplankton: in total, 191 species of phytoplankton were identified. The phylum of phytoplankton include: Bacillariophyta with 97 species (50.8%), Chlorophyta with 28 species (14.7%), Pyrrophyta with 26 species (13.6%), Cyanophyta with 25 species (13.1%) and Euglenophyta with 15 species (7.9%). Bacillariophyta and Euglenaphyta were had the most (51.49%) and the lowest (0.39%) abundance respectively. Nevertheless, Pyrrophyta and Chlorophyta were having the most (53.14%) and the lowest (0.54%) biomass respectively. Mean abundance and biomass of phytoplankton in the photic and the aphotic layers were a significant difference (p〈0.05). The average abundance and biomass decreased with increasing depth (p〈0.05). The most abundant and biomass phytoplankton was determined in the photic layer. Abundance and biomass of phytoplankton in the different seasons have been significant differences (p〈0.05).The most abundance of phytoplankton was in winter, fall, summer and spring respectively (p〈0.05) and the most biomass of phytoplankton was in winter, fall, spring and summer respectively (p〈0.05). Zooplankton: Also, 14 species of zooplankton include: 2 species of Copepoda, 5 species of Rotatoria, 2 species of Protozoa, 3 species of Cladocera, 2 species of newborn and larvae of Balanus and larvae of Bivalvia from meroplankton group were identified. However, the dominant zooplankton population was divided into three groups of copepoda, Rotatoria and meroplankton. Mean abundance and biomass of zooplankton in different seasons and layers showed significant different (p〈0.05). The most abundance and biomass of them in winter and the photic layer was determined. The mean abundance of Rotifera, Cladocera and protozoa in different depth was having not significant differences (p〉0.05). However, there was significantly in other groups (p〈0.05). The most abundance of them was in photic layer. Ctenophore: Average abundance and biomass of Mnemiopsis leidyiwere recorded 63±7 ind.m^-3 and 7.70±2.34 g.m^-3, respectively. The maximum seasonal average of abundance was 150 ± 17 ind.m^-3 while the maximum biomass was 26.59± 4.61 g.m^-3 in summer (p〈0.05). The lowest seasonal average of M. leidyi abundance and biomass were in the winter (6±2 ind.m-3 and 0.07±0.02 g.m^-3, respectively) (p〈0.05).The average annual abundance and biomass of ctenophore were not significant difference in the 8 transects (p〉0.05). The maximum of ctenophore abundance and biomass were belonged to the depth of low than 20m (p〈0.05). The maximum of ctenophore abundance and biomass were recorded in the 5m depth of Anzali with 639 ind.m^-3 and 158 g.m^-3, respectively. Results of biological and non-biological factors showed that significant changes between euphotic and aphotic layers (p〈0.05).There were the most seasonal changes in the euphotic layer. The results showed that the presence of ctenophore is highly related to the presence of the zooplankton and phytoplankton and some non-biological factors. The water temperature is a limiting factor in the ctenophore Fluctuation at the winter, because of food presence (phytoplankton, zooplankton and Nutrients), M. leidyi have had the lowest abundance. Macrobenthos: Three phyla of macrobenthos (arthropoda, annelida and mollusca) include: 5 classes (crustacea, insecta, polycheata, oligocheata and bivalvia), 8 orders, 11 families, 26 genera and 24 species were identified. The most abundance and biomass of orders in the different seasons were belonged to polycheata (annual average: 2452±258 g.m^-2) and bivalvia (annual average: 25±6 g.m^-2), respectively. Macrobenthic abundance in all orders except insecta and oligocheata in different seasons was significant difference. The amount of seabed total organic mater (TOM) from the 5m to 100m stations was with an increasing trend and the highest percentage had in the 50m and 100m stations (P〈0.05). The percentage of seabed TOM was not significant difference in the different transect (P〈0.05). However, seabed grain size had significant difference (P〈0.05) and seabed grain size from the 5m to 100m stations was with a decrease trend in the size (P〈0.05). Macrobenthos and physicochemical parameters of correlation matrix showed that macrobenthos abundance in the between parameters have a positive correlation with TOM. However, it biomass with pH (positive), inorganic phosphorus (positive) and organic phosphorus (negative) have a correlation. Heavy metals: The levels of heavy metals (Cd, Cr, Cu, Fe, Pb, and Zn) of water had lower than standard and acceptable for aquatic life. Significant accumulation of metals such as Co, Cu and Cr in the sediment was observed at transect Sefidrud and Anzali and Ni at transects Sefidrud and Astara in comparison with six other transects (P〈0.05). Petroleum compounds (16PAHs): Concentration levels changes of petroleum compounds in seawater in different seasons was significant (P〈0.05) and in seasons of spring, summer, fall and winter was 56.5, 9.3, 14.9 and 19.3 of percent respectively. However, the concentration values in sediments of the sea in different seasons and transects were not significant difference. Maximum concentrations in summer and in Astara transects (8.02 mg.kg^-1) were determined. In correlation matrix, observed positive correlation between petroleum compounds and Cr, Ni and Pb in sediments of seabed and between petroleum compounds and Zn in seawater. Organochlorine pesticides (OCPs): The OCPs concentration of seawater in different seasons had significant difference (p〈0.05). The pesticides groups of DDT, BHC, Drin and Endosulfan in seawater in different seasons had significant difference (p〈0.05), but, it was not found in Heptachlor group (p〉0.05). The maximum concentration values of OCPs in all groups in seawater were determined in winter (99%) (p〈0.05). The seawater OCPs concentration in comparison of regional and stations were not had significant differences (p〉0.05). The only DDT group in the seabed sediment had significant difference in different seasons (P〈0.05) and the maximum of seasonal average, it was determined in summer (Average: 0.88±062 mg.Kg^-1 and Max: 4.5 mg.Kg^-1). However, in comparison of regional were not had significant differences (p〉0.05). In correlation matrix, the groups of Endosolfan and Drins in seawater and sediment were had equal variance and the changes were included. There was high correlation of between Endosulfan and Drin with DDT group in seawater and with Heptachlor group in the sediment of seabed. Surfactant: Mean surfactant concentration (LAS) between different seasons had significant difference (P〈0.05). The maximum concentrations of LAS were in spring and summer (Anzali transect) was 0.084 mg.l^-1 and 0.082 mg.l^-1, respectively. Also, average concentrations in the spring: 0.06±0.003 mg.l^-1, summer: 0.059±0.004 mg.l^-1, winter: 0.042±0.003 mg.l^-1 and fall: 0.031 ± 0.013 mg.l^-1 was determined. Nevertheless, there was no significant difference in the study area and station (p〉0.05). Based on existing standards, Surfactant concentration in the southern Caspian Sea region was not critical.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Astara ; Anzali ; Tonekabon ; Noshahr ; Babolsar ; Phytoplankton ; Zooplankton ; Mnemiopsis leidyi ; Pollutant ; Physico-chemical parameters ; Macrobenthos ; Sampling ; Temperature ; Salinity ; Phylum ; Bacillariophyta ; Chlorophyta ; Cyanophyta ; Euglenophyta ; Biomass ; Copepoda ; Species ; Protozoa ; Larvae ; Heavy metals
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  • 75
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    Feasibility study on Intensive polyculture of chainess and Indian carps in I.R.IRAN (Giulan province) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25354 | 18721 | 2018-09-13 13:02:58 | 25354 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: 500000 juvenile Indian majour carps (Labeo rohita,Cirhinus merigala, Catla catla) imported (2004) and transferred to the earthen ponds in Shahid Ansari Carp Breeding Center , In order to assess the viability of rearing under the climatic condition of north part of Iran and also achieve some growth parameters from the larval stage to market size.The fry with 300 mg weight, were released in to 8 ponds.specimens were separated to tree experimental categories: 1= (Labeo rohita) , 2= (Cirhinus merigala) , 3= (Catla catla). fishes were fed with artificial food and also with the natural products of the ponds which were enriched with organic fertilizers, after being equally stocked in the ponds .During the experiment, oxygen level ,PH, turbidity, and temperature were measured . Monthly fry samples were taken, for weight and length biometrical analysis and also determination of the weight gain percentage, average daily growth rate, specific growth rate. Results have indicated adaptations and considerable growth in this species, with an increase from the initial weight of 300 mg to an average of 13.5±1.6 mg in 50 days period until the end of November for fingerlings. Also the average weight of growing phase (second year of production) was 647g. For Labeo rohita ,420 g. for Cirhinus merigala and 734g. For Catla catla. The special growth rate(SGR)in Roho was 4.51 %.and 3.9 % for merigal and6.2 % for Catla while for Chinees carps SGR was 4.9, 6.7, 7.6 and 5.8 for silver carp,grass carp, common carp and big head respectively. Results revealed that the Indian majour carps compatibility with the condition in Gilan province was succesfull and the combination of species and optimization of their compatibility were remind for further studies.
    Keywords: Aquaculture ; Iran ; Indian carp ; Growth ; Labeo Rohita ; Cirhinus merigala ; Catla catla ; Juvenile ; Breeding ; Rearing ; Growth ; Larval stage ; Fry ; Artificial food ; Oxygen level ; pH ; Turbidity ; Temperature ; Species
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    Survey of preservatives on shelf life of silver carp burger (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25126 | 18721 | 2018-08-26 13:44:22 | 25126 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: In this research in order to assess the possibility of antioxidant effects in quality protection and increase the shelf alife of fish burgers, ascorbic acid as a antioxidant by natural source used in raw uncoated fish burgers and in order to comparison by vacuum packaging, 3 treatments of uncoated fish burgers produced from cultivated silver carp: 1- burgers by common packaging (control) 2- burgers by vacunm packaging 3- burgers by 500ppm Ascorbic acid. Also in order to comparison BHA+BHT antioxidants (that have synergistic effects to each other) effect by vacuum packaging to prevention of lipid oxidation in semi-fried fish burgers 3 other treatments produced too: 1- burgers by common packaging (control) 2- burgers by vacunm packaging 3-burgers by 200 ppm BHA+BHT antioxidants comparatively to fats of product. All of the burgers after production and freezing conserved in -18°c for 6 months (Raw uncoated burgers) and one year (Semi-fried coated burgers). During the storage period chemical, microbiological, and organoleptic tests were down by three repetition monthly. Although peroxide value in raw uncoated fish burgers were higher than standard range even from first month but it seems this factor is not suitable for quality evaluation of uncoated raw fish burgers. Evaluation of TBA index in raw uncoated fish burgers during storage time showed at the end of storage period TBA index for control, vacuum and ascorbic acid treatments were 6.31, 4.76 and 1.29 mg malonaldehyde/kg respectively and taste scores were 5.11, 5.42 and 6.16 respectively. Results indicate the positive effects of ascorbic acid to prevention of lipid oxidation. By attention to TVN, TBA and organoleptic tests 4 mounths for treatments without ascorbic acid by vacuum packaging preference and 6 months shelf life for ascorbic acid treatment have suggested in -18°c temperature. For semi fried fish burger 28 prevention effect for lipid oxidation but vacuum packaging had 32 (without significantly difference,P〉0.05). so we can for %BHA+BHT treatment this property was recommend the use of vacuum packaging instead of antioxidant treatment. By attention to TVN, pH, PV, TBA and microbiological and organoleptic tests we can suggest the 8 months for shelf life time of semi fried fish burgers. In this product TVN was the only limitation factor and exeeded from standard range at 9th month of maintenance.
    Keywords: Fisheries ; Iran ; Survey ; Silver Carp ; Burger ; Ascorbic acid ; BHA ; BHT ; Lipid ; organoleptic temperature ; Temperature ; Vacuum packaging ; Common packaging ; Fish
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    Study of effect of nereis diversicolor in growth and survival of Acipenser persicus larvae (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25390 | 18721 | 2018-09-14 07:50:08 | 25390 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The present research aimed to study effect of Nereis worm in feeding, growth, survival and carcass biochimical compositions of persian sturgeon larvae. Five diets including zooplankton (100%) as the control (Z), Nereis diversicolor worm (100%), a mix of Nereis and zooplankton (50% for each), a mix of Nereis, zooplankton and concentrate food (33% for each) with 3 replicates were established. At first, Nereis worms were cultured up to the weight of 200 mg according to the protocol done in the International Sturgeon Research Institute. Required zooplanktons were obtained for Dr. Beheshti Sturgeon Rearing and propagation center. Concentrate food was also made in the ISRI. Persian sturgeon larvae with the average weight of 95.66 mg were put in 60-Liter tank (60 larvae in each tank) under the same physical and chemical parameters of water. 8 days after the beginning of active feeding, they were fed five times a day with experimental dites based on 20-30% of their weight for 15 days. Water quality parameters, such as dissolved oxygen, temperature, pH values were recorded daily. The average of temperature, pH and dissolved oxygen during the test were 22.8±1.3ºc, 7.5±0.1 and 6.58±0.9 mg/l respectively. At the end of this period, condition factor (k), specific growth rate (SGR), food conversion rate (FCR), weight gain (WG) and Body weight index (BWI) were calculated. Total fat and fatty acids profile were analyzed. The results showed that there was a significant difference between growth indexes and food consumption (p〈0.05). BWI, GR and SGR indices showed no significant difference between N and NZ treatment, but the average of these indices were higher in NZ treatment. The most and the least average of SGR, BWI were observed in NZ and NZC treatments respectively. Condition factor showed no significant difference in all diets except NZ. FCR had no significant difference between N and NZ as well as after diets. The highest and the lowest survival rate was observed in N treatment (96.11±1.46%) and NZC (85.55±3.37%) respectively. The results of carcass analysis showed that there is a strong correlation between fatty acids of body and fat resources of diets. According to the significant difference in growth rate between treatments (p〈0.05), the larvae fed with N diet presented a better performance compared with others. These results indicate that cultured sturgeon larvae need n-3 and n-6 series of fatty acids in their diet.
    Keywords: Aquaculture ; Iran ; Acipenser persicus ; Nereis diversicolor ; Growth ; Survival ; Fatty acids ; Larvae ; Feeding ; Persian sturgeon ; Zooplankton ; Dissolved oxygen ; Temperature ; pH ; SGR
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    The study on culture of rainbow trout (Oncorhynchus mykiss) by new design of foster-lucas ponds (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25384 | 18721 | 2018-09-14 07:53:44 | 25384 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Intensive culture of rainbow trout, usually done using several different methods. The objective of this study was to compare fish performance and welfare in a new design of Foster-lucas ponds (FLP) and a raceway ponds (RP) under field conditions. Currennt study was designed in two treatment with three repeats. Each pond was randomly stocked by rainbow trout fish with initial average weight of approximately 51±5 g. stocking density was 120n/m3 during study. After 10 months of trial, fish fed diets with commercial diet (Chineh Company). Feeding operation conducted according to the manuals by consideration of water temperature, fish weight and biomass. During the 10 months experiment, the fish survival rate was high (97.39 and 98.05%). No significant differences in growth parameters were observed between RP and FLP, but in all parameters FLP was higher than RP Final weight was 0.99% higher in FLP than in RP. Water quality factors (Dissolved oxygen, PH) was better in FLP. The results confirm that when water quality is maintained in safe level ranges, growth rate of rainbow trout attained in FLS are comparable to those in RP.
    Keywords: Aquaculture ; Iran ; Rainbow trout ; Oncorhynchus mykiss ; Fish culture ; New design of Foster-lucas pond ; Raceway ponds ; Culture ; Stocking ; Density ; Diet ; Feeding ; Temperature ; Biomass ; Growth ; Dissolved oxygen ; pH
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    Study of physico-chemical Characteristics of water in the southern of Caspian Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25387 | 18721 | 2018-09-20 11:19:25 | 25387 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This study was conducted to physico-chemical characteristics of water in the Iranian coastal of southern Caspian Sea during four seasons at 8 transects (Astra, Anzali, Sefidrud, Tonekabon, Nowshahr, Babolsar, Amirabad and Bandar Turkman) during 2009-2010. 480 samples were collected at different layers of water and then analyzed based on standard methods. Results showed that the mean of water temperature were 18.67±0.32 and 17.82±0.43 ◦C at the surface and euphotic layer, respectively. Minimum and maximum of water temperature were recorded in winter (6.40) and summertime (27.60). In addition, temperature gradient was ranged between 12 to 15 ◦C at 20 to 50 layers in 50 and 100m depths. Mean of salinity was 11.04±0.17 g/l at euphotic layer. Pearson correlation between temperature and salinity was positively significant. Therefore, maximum salinity was recorded in summer and minimum in winter. Mean of transparency (SD) in the present study was 4.35±0.21 m which compared to the pervious sampling period (2008) showed small decrease, but the mean of pH was observed 8.43±0.01 which was higher than the previous sampling period (2008). The mean of DO and DO% were observed 5.72±0.06 ml/l and 130±1 percent at euphotic layer during sampling period. The mean of DO% was recorded 104±5 at euphotic layer in 1996 (before introduction of Mnemeiopsis leidyi), but the study in the years 2004, 2008 and present (one decade after introduction of Mnemeiopsis leidyi) this value was registered higher than 120% at euphotic layer. This shows that trophic status of Caspian ecosystem shifted from oligotrophy (before introduction of Mnemeiopsis leidyi) to meso-eutrophy (after introduction of Mnemiopsis leidyi). Annual concentration of inorganic nitrogen (DIN= NH^4 +, NO^2-, NO^3-) has a fairly wide variation. Percentage of nitrogen components out of DIN were varied 9-98, 0.2-28.2 and 0.0-90.0 respectively. In this study, percentage of DIN was lower than 15% and organic nitrogen (DON) was higher than 80%. Overall, results showed that water temperature, salinity, transparency and DON were recorded lower than the previous study (2008), but DO, DO%, pH, NH^4+, NO^3- and DSi were higher than the previous sampling period. Inorganic phosphorous (DIP) and NO^2- have not changed substantially. N/P ratio of Caspian Sea has a narrow range which order of magnificent lower than other seas. The results of the present study showed that Caspian ecosystem was nitrogen limitation before introduction of Mnemiopsis leidyi, while it seems that after introduction of Mnemiopsis leidyi the system has been shifted to the phosphorous limitation.
    Keywords: Ecology ; Iran ; Caspian Sea ; Astra ; Anzali ; Tonekabon ; Nowshahr ; Babolsar ; Sefidrud River ; Physico-chemical parameters ; Nutrients ; Water ; Temperature ; Salinity ; Euphotic layer ; Sampling ; Mnemeiopsis leidyi ; Oligotrophy
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    Study of increasing production in trout rearing farms with focusing on water aeration systems and water physical filtration aspect in west Azarbaigan Province (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25426 | 18721 | 2018-09-23 17:19:13 | 25426 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This research was carried out to determined water reuse effects on growth indices of rainbow trout. For this reason 4 groups includes: control (with use of 100% fresh water), Test 1(rearing with use of 30% recycled water (with physical filtration) and 10% fresh water), Test 2(using of 10% recycled water (with physical filtration) and 30% fresh water), Test 3(using of 100% recycled water) was done. Fish stock of each groups were done with 62 fish in each square meter and average weight (15±1.5).The Growth index, SGR, CF, FCR, SR between different groups in each month was calculated and determined. In this research comparison the average of month growth increment between different groups indicated that significant decrease of growth in test groups with control group and also 1 and 2 groups with 3 group (p〈0.05). There were not considerable differences to comparison this factor between groups 1 and 2(p〉0.05). To comparison FCR, SR, MR has shown a significant difference between different groups to each other (p〈0.05). These results shows that with increasing the replacement of water in rainbow trout culture ponds with recycled water cased significant decrease in SR and CF and significant increase in MR and finally decrease in production and density per unit area (p〈0.05).
    Keywords: Aquaculture ; Iran ; Azarbaigan province ; Oncorhynchus mykiss ; Reused water ; Growth index ; Rearing ; Trout ; Rainbow trout ; SGR ; Culture ; Density
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    Studying molecular 2 species econimic shrimps (P. merguiensis, P. indicus) Northern coast of the Persian Gulf and Oman sea using microsatelitte markers to separating and identificating of their possible populations (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25434 | 18721 | 2018-09-23 16:52:05 | 25434 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This study focuses on molecular investigation of two commercial shrimp species of penaeus family namely as : P. merguiensis and P. indicus in order to find and introduce the genetic differentiations and also probable genotypes for monitoring and managing the genetic resources of populations in three major catch areas in the Persian Gulf and the Oman Sea. Only five out of the eight primers for P. merguiensis and four out of the eight primers for P. indicus produced good amplified PCR products with fixed annealing temperature. The rest of the primers were either not easily amplified or produced nonspecific bands. Seven and six alleles were found to be unique to each of the three populations in P.merguiensis and and two populations of P. indicus respectively. Occurrences of heterozygosity deficiency were found at most loci. These heterozygosity deficiencies in observed heterozygosity in compare to expected heterozygosity may be due to inbreeding, genetic drift and consequences of illegal overharvesting of P. merguiensis and P. indicus in the studied areas as well. Deviation from HWE in both studied species was significant in most microsatellite loci (P 〈0.001). We observed deviation from HWE in most loci with hetrozygosity deficits. The genetic variation results showed that the pairwise Fst values were significant between populations in both species. The assignment test for P. merguiensis revealed high gene flow between Hormoz and Jask and restricted genetic flow between Guatr and Hormoz populations .We observed high gene flow between Hormoz and Jask populations for P. indicus. It seems that the changes in immigration patterns of populations between Hormoz, Jask and Guatr areas in both species are depend on the influence of Persian Gulf currents or the life cycle of studied species. Alternatively, the presence of ecological barriers such as mangrove forests may result in restricted genetic flow between Guatr and both Hormoz and Jask populations.
    Keywords: Aquaculture ; Biology ; Iran ; Persian Gulf ; Oman Sea ; Hormoz ; Jask ; Guatr ; Microsatellite markers ; P.merguiensis ; P. indicus ; PCR ; Molecular ; Species ; Genotype ; Population ; Temperature
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    Sperm bank creation of Caspian Sea trout (Salmo trutta caspius) breeders (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25461 | 18721 | 2018-09-26 10:23:40 | 25461 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The Caspian Sea trout (Salmo trutta caspius) is distributed in southern basin of the sea and the natural stocks of this fish is critically endangered. The present study was conducted through the breeding seasons of Caspian Sea trout in January 2012. Estimation of sperm male potential was carried on samples collected from a total of 12 male spawners which included 8 samples (caught 3 years ago) and 4 samples (freshly caught). Semen samples were collected after anesthetized with clove powder for 10 minutes at water temperature of 9-9.4 °C. The differences of mean semen volumes, sperm motility time, sperm density and the percentage of sperm motility from freshly caught spawners (5.5±3.4 ml, 35.5±8.7 sec, 4.3±0.7×109 ml^-1, 32.5 ± 8.5% respectively) were significantly higher than 3 years caught spawners (1.5±0.93 ml, 26.5± 2.8 sec, 1.98±1.6×109 ml^-1, 25.7±9.7 % respectively) (P〈0.05). Also the results showed that there are not significant differences in mean percentage of motility and motility duration between freshly caught sperm from fresh broods and that’s from previously caught (among fresh sperm samples, one, three and eight months of cryopreservation) (P〉0.05).
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Sperm cryopreservation ; Percentage of motility ; Sperm bank ; Salmo trutta caspius ; Breeding ; Samples ; Spawners ; Temperature
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    Rainbow trout broodstock and progenies registration and selection of Yasouj Fishery Research Center in order to breeding (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25456 | 18721 | 2018-09-25 18:08:14 | 25456 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Frequently,the development of quantitative traits in livestock based on breeding programs has been more important. In spite of higher selection response in fish than in farm animal, it is no progress in fish breeding programs in some regions such as IRAN, because of little information of genetic variation of stock, disconstructed or undesigned base population, the deterioration of genetic resourse and don’t well informed educated researchers, extension workers and aquaculturist in breeding theory and its practical issues. At first step, in Yasouj Coldwater Fishes Breeding Research Center, in order to conducting combined selection program in rainbow trout broodstock as base population and their off springs in mixed age parents,150 female and male broods with higher mean weight were selected, striped in 6 stage and eggs were incubated. One-year Fishes(45000 pcs.) of the six groups with higher mean weight in 5 stage were selected(438 pcs.) and remainder was discarded. Before selection, a few fishes of six aged-groups as control group were cultured apart. Difference (p〈0.05) induced between mean weight of the selected groups and with control group was because of age difference in them and of no deletion in control group(don’t throw out small individuals) by selection. The whole groups don’t have significant difference (p〉0.05) in FCR and FER. Based on results, it isn’t told that difference between experimental and control groups is a result of genetic improvement of growth rate trait induced of selection process in one generation and the continue of this program for several generations in order to reveal the development of a quantitative trait is unevitable and mating of selected broods (438 fish) in a crossbreeding program and the selection of off springs is essential.
    Keywords: Aquaculture ; Iran ; Yasouj ; Rainbow trout ; Breeding ; Selection ; Broodstock ; Genetic variation ; Genetic ; Growth Rate ; Generation
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    A survey on the effect of Cochlodinium.sp bloom on shrimp culture complexes and hatcheries activities in Bushehr Province (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25457 | 18721 | 2018-09-25 11:07:08 | 25457 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: The aims of this project was to inform and aware about the red tide condition before entering the algal bloomer in water resources to the hatcheries and farmed shrimp complexes in Bushehr province coastal. Field investigation and sampling have been carried out in the southern of the input water channels of Mond, Delvar and Helleh farmed shrimp complexes, which are located along the coastal of Bushehr Province, from April to December 2011 .The physic-chemical parameters such as pH, salinity and water temperature and also meteorological conditions were measured and recorded on the field. The water sampling has been lunched for determination of nutrients and chlorophyll - a, phyto- and zoo- planktons. No Cochlodinium.sp outbreaks have been detected in the studied area, during the study. The only bloom which reported by a fisherman, was outbreak in offshore of Bushehr province in Khoure Khan on 13th September 2011. The sample which transferred to the Iranian National Shrimp Research Institute was included Alexandrium.sp and its density was 2 million cells per liter. The identified phytoplanktons were belonged to three order of Bacillariophyceae (52.6%) with average density of 10778 cells per liter, Dinophyceae (37.7%) with density of 7731 cells per liter and Cyanophyceae (9.7%) with density of 1980 cells. 12 genera belonged to Dinophyceae , 25 genera of Bacillariophyceae and two genera of Cyanophyceae were observed during the study. The highest density of phytoplankton was recorded in Helleh station by 18374 cells per liter. The maximum density of phytoplankton was at Delvar station by 141120 cells per liter in December. The highest density of the phytoplanktons was belonged to Dinophyceae by 126000 by cells per liter of which the Alexandrium.sp had the density of 124500 cells per liter in August 2011. From the Dinophyceae the Alexandrium.sp with mean density of 20345 cells per liter, Ornithocercus 920 cells and Prorocentrum.sp 820 cells were the predominant species. The identified Zooplankton in were belonged to 8 branches and 19 groups. The highest density of zooplankton was recorded in Helleh station by 1194 no. per liter. Nauplii were the dominant zooplankton groups with an average density of 136.4 no. per liter, Tintinnids 98.7, Cyclopoida 60.8, Calanoida 35.7 and Harpacticoids 14.5 no. per liter . The average of water and air temperature was recorded 29.4 °C and 28.3 °C, respectively. Average of salinity was 41.2 and pH was 8.46. The average of depth in all stations was 5.7 m. The mean concentrations of silicate, nitrate, nitrite, ammonia and total phosphate were 1.99, 0.03, 0.009, 0.14 0.15 ppm, respectively and the average of chlorophyll - a was 0.94 mg.m-3.
    Keywords: Aquaculture ; Iran ; Bushehr Province ; Delvar ; Helleh ; Cochlodinium.sp ; Shrimp Culture ; Bloom ; Survey ; Hatcheries ; Sampling ; pH ; Salinity ; Temperature ; Chlorophyll a ; Phytoplankton ; Zooplankton ; Alexandrium.sp ; Bacillariophyceae ; Dinophyceae
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    A study on water quality and physio- chemicals parameters in surface water, underground water and wastewater of Mangol dam basin (Mazandaran province) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25471 | 18721 | 2018-09-26 09:48:04 | 25471 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This study was conducted to determine water quality of surface water (7 stations), ground water (5 stations) and wastewater (4 stations) at Haraz River during instruction of Mangol Dam in 2009-2010. This study is to follow physico-chemical fluctuation of waters and compared the data obtained with previous studies and standard levels. Results of current study showed that annual mean with standard error of temperature, DO, BOD_5, COD, HCO^3-, CO_3^2-, TA, Ca^2+, Mg^2+, TH, TSS, TDS, Cl^-, PO_4^3-, NH^4+, NO2- and NO3- were observed 12.7 (±0.61) centigrade, 10.13 (±0.12), 2.1 (±0.1), 7.71 (±0.99), 28.1 (±6.1), 14.34 (±1.64), 36.30 (±2.33), 64.66 (±2.34), 28.84 (±1.52), 271.62 (±8.41), 0.37 (±0.03), 0.30 (±0.02), 28.02 (±1.96), 0.06 (±0.01), 0.010 (±0.001) and 0.94 (±0.03) mg/l, pH was 8.00 (±0.02) and EC 0.50 (±0.02) ms/cm in surface water. The annual mean with standard error of temperature, HCO^3-, CO_3^2-, TA, Ca^2+, Mg^2+, TH, TSS, TDS, Cl^-, PO4^3-, NH^4+, NO^2- and NO^3- were observed 21.62 (±1.03) centigrade, 54 (±5), 11 (±5), 57 (±5), 208.6 (±42.5), 109 (±23), 1088 (±165), 0.065 (±0.017), 0.97 (±0.11), 317.8 (±76.2), 0.25 (±0.06), 0.06 (±0.01), 0.0007 (±0.0001) and 0.78 (±0.05) mg/l, pH was 7.37 (±0.07) and EC 1.98 (±0.23) ms/cm in underground water. In addition, the annual mean with standard error of temperatute, DO, BOD5, COD, TSS, TDS, and NO3- were observed 12.7 (±0.61) centigrade, 13.59 (±0.87), 9.80 (±0.12), 2.22 (±0.18), 7.54 (±1.02), 0.50 (±0.03), 0.320 (±0.008) and 0.990 (±0.016) mg/l, pH was 7.95 (±0.02) and EC 0.64 (±0.01) ms/cm in wastewater. Quality of surface water of Haraz River and its branches were almost contained suitable for usual applications, but sometimes the quality of surface water based on BOD5 need to more attention for man uses (such as supply of drinking water). In addition, the quality of surface water was relatively suitable for aquatic based on the aquatic standard levels. Results also showed that water quality index (WQI) was declined from upstream to downstream and some parameters were not in standard ranges. The quality of surface water classified in group II at all stations based on water classification rules which it shows serious water quality changes due to environmental destroyed and domestic, agriculture sewage pollutions. Groundwater were affected by different springs at middle and downstream which they caused change physico-chemical characteristics and water quality. Wastewater was classified from good to moderate based on BOD5. The quality of wastewater was declined at downstream with decreasing DO and pH and increasing BOD5 and pollutants.
    Keywords: Ecology ; Iran ; Mazandaran province ; Mangol Dam basin ; Haraz River ; Physico-chemical characteristics ; Surface water ; Ground water ; Wastewater ; Temperature ; Aquatic ; Agriculture ; Pollution ; pH
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    Investigation of breeding and culture in Iranian cichlid (Iranocichla hormuzensis) as endemic and ornamental species (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25477 | 18721 | 2018-09-26 10:23:45 | 25477 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Iranian Cichlid is an invaluable ornamental species which is domesticated in Iran and called Iranocichla hormuzensis scientifically, is living in the Hormoz zone, Shahou River (between Bandar Abbas and Haji Abad). The first phase of the research project started in September of 2012 and lasted about 18 months, focused on adapting the wild cichlids to cope with the aquarium environment. Therefore, about 63 male and female of Iranian cichlids collected from the natural habitat, Shahou River, then moved to the nearest city, Bandar Abbas to and released in aquariums which were filled by water of River where they were living. After few days they moved to “innovative aquaculture technologies research station” and reared in 100 liter glass tanks to monitor their behaviors. By initial days, the Cichlids seemed to be stressed out significantly, they were flashing, hitting them to the aquarium, trying to jump out and get discolored due to strange environment. By days, the stressful treats decreased and the experiments coped with the new environment. At the next step, schools of 5-7 fish organized to pear up. The mortality rate collected daily and accidental biometry recorded fortnightly. The rearing temperature adjusted and was stable at 37˚C. Finally, the Cichlids were completely adopted and prepared for mating and breeding.
    Keywords: Aquaculture ; Iran ; Bandar Abbas ; Hormoz ; Shahou River ; Breeding ; Culture ; Iranian cichlid ; Iranocichla hormuzensis ; Endemic species ; Ornamental species ; Aquarium ; Mortality ; Rearing ; Temperature
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    Investigation of two crop culture of western white shrimp (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25492 | 18721 | 2018-10-05 07:01:09 | 25492 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Two crop culture of western white shrimp with 2 treatments and 2 replicates in each treatments in earthen ponds with area 0.4 hectares at a density of 20 pl 18 in each square meter were done. For the culture of shrimp in the second stage, the post larvae cultured in nursery pond with area of 0.25 hectare, and density of 96 post larvae in each square meter, and then transfer to the rearing ponds. In each pond, 2 aerators with potency of 3 horsepower fixed. Every day horsepower clarified at 21p.m. to 23 p.m and out at 6 a.m to 7 a.m. The first stage of culture begun in 19 April with pl 18. The yield pick-up after 96 days. The stocking of pl in the nursery ponds was done in 25 July with pl16 and after 38 days hunted and transferred to second stage culture. The second culture was done in 1 September and pick-up after 65 days. For feeding of shrimps we used commercial food numbers 4001 until 4006. In the first mount the feeding of shrimps were done as blind feed and then with the shrimps biomass and with research of feeding trays. Water temperature, dissolved oxygen and pH daily in 2 times at the morning (6 a.m) and evening (at 15 p.m) and the salinity, transparency and water depth 1 time daily measured. In the first culture period (96 days), the mean weight, length, daily weight gain, FCR, survival percent shrimp production were 14.03 gr, 12.08 cm, 0.15 gr, 1.02 gr, 92 percent and 2132 kilograms and in the second culture period (65 days) 14.55 gr, 12.48 cm, 0.22 gr, 0.84, 73 percent and 2675 kilogarms measuered respectively. Totally, in the second period culture the initial weight of shrimps was higher than the first period culture 1.95 gr and 0.008 gr respectively, therefore growth indexes in the second period were better than the first culture period. Coefficient of determination of weight and lenth of shrimps with increase of cultue days, in the first and second culture periods, in the total cases the number were higher than 0.97. This shown that the role of increase of days on the increase of weight and lenth is higher than 97 percent. Also, determination of product moment, showed that there is very strong correlation between increase of days and increase of weight and lenth and between weight and lenth (0.97 to 0.99).
    Keywords: Aquaculture ; Iran ; Western white shrimp ; Two crop culture ; Earthen Pond ; Density ; Larvae ; Nursery pond ; Feeding ; Temperature ; Dissolved oxygen ; pH ; Salinity ; Transparency ; Weight gain ; FCR ; Survival
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    Study on some physico-chemical parameters in the southern of Caspian Sea- Mazandarn Province (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25490 | 18721 | 2018-10-05 07:30:52 | 25490 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This study was conducted to determine physico-chemical characteristics of water and their spatial and temporal fluctuations in the Mazandaran coastal of Caspian Sea in 6 months, four seasons at 4 transects (Tonekabon, Nowshahr, Babolsar, Amirabad) during 2012-2013. 72 samples were collected at surface layer of water in 5, 15 and 30m depths. Then the sampls analyzed based on standard methods. Results showed that the mean of water and air temperature were 19.46±0.85 and 20.25±0.78◦C, respectively. Minimum and maximum of water temperature were recorded in winter (7.00) and summer time (28.10). Mean of salinity was 12.35±0.13 ppt. The maximum salinity was recorded in summer and minimum in winter in all transects. Mean of transparency (SD) in the present study was 2.63±0.18m. The mean of pH was observed 8.51±0.02 which was higher than the previous sampling periods. The mean of DO was observed 6.00±0.07 ml/l during sampling period. Annual concentration of dissolved inorganic nitrogen (DIN= NH4 +, NO2-, NO3-) has a fairly wide variation in different months and transects. Percentage of nitrogen components out of DIN were varied 6-53, 0.14-26.0 and 37.0-94.0 respectively. In this study, percentage of DIN was lower than 15% and dissolved organic nitrogen (DON) was higher than 80%. Mean of annual dissolved inorganic phosphorous (DIP) and organic phosphorous were 0.58± 0.04 and 0.48± 0.02 µM, respectively. DIP and DOP percentages recorded 54 and 46 respectively. Mean annual of dissolved silicon (DSi) obtained 9.5± 0.2 µM. Based on the results, the system was in phosphorus limitation during spring and summer but it shifted to nitrogen limitation in autumn and winter. Phytoplankton development was not limited by DSi at any seasons.
    Keywords: Ecology ; Iran ; Caspian Sea ; Mazandran Proviece ; Physicochemical parameters ; Nutrients ; Samples ; Temperature ; Salinity ; Transparency ; pH ; Dissolved inorganic nitrogen ; Phytoplankton
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    Cryopreservation of rainbow trout (Onchorhynchus mykiss) semen (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25512 | 18721 | 2018-10-05 15:26:26 | 25512 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Breeding has moved beyond the point of simply propagating random stock. Genetic potential can be improved by establishing and crossing genetically defined strains, crossing species and manipulating gametes. Some of these activities require cryopreservation of semen. Cryopreserved sperm could also be a means of exchange of genetic material between locations and populations when transportation of live fish is restricted. In this study we had a survey on rainbow trout sperm quality in Genetic and Breeding Center for Coldwatwr Fishes, Shahid Motahari, Yasouj. Then Cryopreservation trials on rainbow trout (Oncorhynchus mykiss) sperm were carried out using six basic extenders. Egg batches of 25 g were inseminated with semen frozen in five 0.5-ml straws. Thowing temperature of cryopreserved sperm also was tested. In anather trial we investigate different cryoprotectant. The result showd that the best period for sperm collection in this center was from Novamber to January. The best extender based on sperm motility parameters were extender 2 and 1 with 57.2 and 56.9% fertilisatin rates, respectively. So this two extender were used in the further experiments. The best thowing temperathre was 25°C in 30s. Testing different cryoprotectant, adding 10% methanol to extender 1 gave the highest fertilization rate (64.6%) among cryoprotectant that used with this extender. In the other extender (2) adding 5% DMSO and mixture of 5% DMSO and 1% glycerol gave the highest fertilization rates (64.8% and 67.0%, respectively). In conclusion using extender 1 and 2 with mentioned cryoprotectant and thowing rate of 25°C in 30 s was recammended for rainbow trout sperm cryopreservation.
    Keywords: Aquaculture ; Iran ; Yasouj ; Sperm cryopreservation ; Rainbow trout ; Extender ; Cryoprotectant ; Onchorhynchus mykiss ; Breeding ; Genetic ; Coldwater ; Temperature ; Fertilization
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    The study and feasibility of Cheshmeh-Ali River and downstream area of Shahid Shah-Charaghy dam in Damghan for aquaculture development (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25523 | 18721 | 2018-10-05 15:26:23 | 25523 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: To reveal the fisheries productivity and to get the maximum possible exploitations of the running water resources, existing potentials for aquaculture in Shit and Kordabad rivers in Tarom of Zanjan province were surveyed in a one year round study. In this way 4 and 3 sampling site were respectively selected in Shit and Kordabad rivers and benthic macroinvertebrates and water samples were collected and analyzed at least two times a season and fish fauna were also sampled and studied only two times during the period. All the measured water quality characteristics(such as O_2, water temperature, pH, nutrients, NH_3, BOD_5 and . . . ) were within the normal range suggested for the cold water fish culture. In both rivers no significant differences (p≤95%) were detected for physicochemical parameters within the study sites except for electrical conductivity and total hardness which differed in the firs and last station. Depletion of Agricultural run off and residual from the adjacent farmlands and municipal drainages into the water body of the rivers, were certainly effective in these changes However none of water quality parameters exceed the maximum permitted level for aquaculture. According to the benthic macroinvertebrates study 38 family of 12 taxa and 26 family of the 11 taxa were identified in Shit and Kordabad rivers respectively, most of them belonged to the aquatic insect larvae of the sensitive groups. Community structure indices and Hilsenhof family level biotic index proved a "very good" to "good" water quality criteria for the study sites in both rivers .One fish species in Shit and five species (4 of them were endemic) were identified in Kordabad river. Despite acceptable water quality in Shit river, some inhibiting factors such as adjacent farmlands and dense agricultural land use, high slope of river valley in most region which cause rare suitable lands for locating aquaculture sites, very limited access to road and electricity supply and lack of acceptability to manipulate or divert river water for fish farms, by the local people, are the major constrains for constructing trout farms in Shit river. However constructing a 15 mt capacity rainbow trout farm is possible at first 2 kilometers of the upstream where the water quality and quantity are relatively better and there are some lands with low slope and access to road and electricity supply. Kordabad river has a similar criteria and water quality and quantity at upstream is desirable for rainbow trout culture. The first 2 kilometer of this river is capable of constructing trout farms in some non cultivated lands. Moreover the Kordabad natural dam reservoir is available to conduct its water for further demands. In addition a total of 75mt rainbow trout production in the form of 2 or 3 separate site is possible in this river. Even though the local climate particularly in Kordabad river is acceptable for carp fishes, however land limitations, soil texture and the high volume of water demands, make warm water fishes commercial production impossible.
    Keywords: Aquaculture ; Iran ; Zanjan province ; Damghan ; Running water ; Aquaculture ; Benthic macroinvertebrates ; Rainbow trout ; Temperature ; pH ; Nutrients ; Water quality ; NH3 ; Species
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    Introduction of Litopenaeus vannamei to shrimp culture industry of Iran (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25194 | 18721 | 2018-09-03 17:35:27 | 25194 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Various Problems in front of shrimp culture, mainly in biological fields and restrictions of production cycle, emphasized the species diversity in aquaculture industry of Iran in fourth socio- economic development plan of fisheries department. By this means, the Iranian Fisheries Research Organization, defined a Programme with four sub- projects, and started the activities on beginning of 2004. On the year 2004, 80 pairs of SPF/SPR Litopenaeus vannamei broodstocks imported from Hawaii, but late importation and prolonged acclimatization period, caused propogation and PL production on mid-September. Despite of terminated culture season, some PL's stocked in earthen ponds of Helleh site in Bushehr province. Results of this phase showed that, L.vannamei could support the cold winter of about 9 C temperature and about 20000 broodstocks harvested at the next year. At the next year, 55 pairs of SPF/SPR broodstocks imported by the same way, and after production of PL in Bandargah hatchery, the farming carried out in Helleh research earthen ponds. At this year, shrimp farms of Bushehr province infected with WSSV, but L.vannamei growth was very good, and gained more than19g after 90days farming. On the year 2006, the farming pilot project repeated, and the way for extension smoothed. In a parallel action, introduction of L.vannamei to Choebdeh site (Khouzestan province) showed remarkable results after 5 years collapses by WSSV. The results of broodstocking, breeding and farming research projects transferred very soon to shrimp producers, as the production of L.vannamei in Khouzestan and Bushehr provinces raised to 2575tons in the year 2008. In addition the condition for introduction of this species to Golestan province smoothed on 2008, and stocking at rate of 5-6 individuals per m2 in earthen ponds of Gomishan resulted in 20g. Shrimp weight after 75 days culture. This stock used as brood stock for commercial production on 2009 by private sector. It seems that all four southern provinces are going to utilize L.vannamei as the main species in 2009, and domesticated brood stocks.
    Keywords: Aquaculture ; Iran ; Bushehr province ; Khouzestan province ; Litopenaeus vannamei ; Introduction ; New species ; Shrimp ; Culture ; Diversity ; Aquaculture ; Broodstocks ; Temperature ; L.vannamei
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    Estimation of hormone residuals (B-Estradiole) in rainbow trout (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25558 | 18721 | 2018-10-05 16:28:57 | 25558 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: Residual level of 17- Beta Stradiol and progesterone hormones in rainbow trout fish plasma were measured during different period using RIA method. Blood sampling from abdominal aorta were taken from 70 individual of female fishes (100±11 g) which had been exposed to hormone at 0.5, 1,2,4,8,12,24 and 168 h (7 groups) compared with control group which was not exposed to this hormone. Results showed that plasma hormones measurement in different fish groups after exposing had significant differences (P〈0.01) and the highest and lowest 17-Beta Stradiol hormone residue were observed in fishes that exposed 0.5h and 168h to hormone respectively (121±9 ng. ml^-1 and 3±0.9 ng. ml^-1) but there is no any differences between fishes exposed 168h to hormone and control group. Also the highest progesterone hormone level were measured in fishes 0.5 an 1h exposed and the lowest one was in fishes 168 h exposed. The range of progesterone hormone were between 0.3 to 1.1 ng .ml^-1 and significant increasing of this hormone levels were obtained in fishes exposed to hormone 4 to 24h (P〈0.01). As consequence these hormone can not residue in fishes for a long time and maximum after one week the levels back to the normal.
    Keywords: Aquaculture ; Iran ; 17- Beta Stradiol ; Rainbow trout ; Hormone residue ; Estimation ; B- Estradiole
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    Effects of medicinal plants Origanum vulgare L. and Aloe vera on immune system of rainbow trout (Oncorhynchus mykiss) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25571 | 18721 | 2018-10-06 16:38:40 | 25571 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: In recent years, according to increase in consumption of fish in the world, however, due to the lack of control of many common diseases with disease-causing pathogens, the total world production of fish is threated in aquaculture industry. Rainbow trout (Oncorhynchus mykiss) is one of the most preferred species in aquaculture of Iran. Development of an economical artificial to accelerate the growth and to maintain the health status of this fish is of major importance for sustainable rainbow trout culture. Fish diseases are a serious threat to economic viability of any aquaculture practice. Currently, the commercial aquaculture industry prefers to reduce the costs of production. Because the cost of antibiotics used for prevention and treatment of diseases, and excessive use of growth hormones for improving growth performance is very high. However, the development of antibiotic resistant bacterial strains, accumulation of residue in cultured fish and environmental problems associated with the use of chemicals have led to investigate on suitable methods of disease management. Therefore, a new approach to immunotherapy is actively used to prevent or treat fish diseases, increased disease resistance, feed efficiency and growth performance of fish in a sustainable aquaculture industry. In this regard, extensive research has been carried out to test the new compounds led to the development of the aquaculture industry. It has been proved that use of medicinal herbs in fish diet enhance the immune system against infections with various bacteria, especially, Aeromonas hydrophila in different species of fish, which is of the major bacterial pathogens, leading to heavy mortality rate and decrease the productivity efficiency, causing high economic loss of the fish farmers. The aim of this study was to evaluate the effects of aqueous-alcoholic extracts of two medicinal herbs, Origanum Vulgare L. and Aloe vera on the immune system of rainbow trout (Oncorhynchus mykiss). Aqueousalcoholic was prepared by percolation method and concentrated. After, they were dried by dry evapory. The study was conducted in two stages. First, 1200 of rainbow trout (Oncorynchus mykiss) fry with an average initial weight of 13 ±0.05 g, and the second, 2400 of fry rainbow trout with an average initial weight of 2±0.13 g. At each stage, the fish in 4 groups: 1) placebo-treated group (negative control), 2) treatment with Origanum vulgare extract, 3) treatment with Aloe vera extract and 4) treatment with levamisole (positive control). Each group was consisted of three replicates. Fish were distributed in 12 circular concrete tanks with volume 1000 liters of water and flow 2.5L per second. In the first phase were used of 100 fish 13 grams and the second phase of 200 fish 2 grams. During the experiments, a number of physico-chemical factors, including water temperature, dissolved oxygen and pH were measured. In this study, Origanum vulgare, Aloe vera extracts and placebo (70 % lactose, 10 % starch and 20 % talc) were used at a rate of 1% and levamisole at a rate of 0.1% of weight feed at a rate 2% of body weight. At each stage of the experiments, each group of fish were fed once a day and in the first feeding for 10 weeks. Other promises were fed with regular food (no additives). During the experiments, the fish were weighed weekly throughout the biometry and measured every 2 weeks. To measure the weight, total biomass of fish from each replicate was divided the number of fish in the same replicate. To measure the length from each replicate 15 fish were randomly netted and anaesthetized with 50 mg/L of tricaine methanesulfonate (MS222, Sigma Chemical Co. St. Louis, MO, USA) and then the mean was calculated. During bleeding, fish were rapidly netted, tranquillized with 50 mg/L of MS222. Fish 13 gram were bled from caudal vein using 1 ml insulin syringe fitted with 24 gauge needle and 2 gram by cutting the caudal. To minimize the stress to fish, 1 ml of blood was drawn and the whole bleeding procedure was completed within 1 min. A total number of 15 blood samples were collected from 15 fish from each group (5 fish from each replicate) at the end of every 2 weeks, 24 h after final feeding period for the analysis of the hematological and serological parameters. The blood pooling of 5 fish from each replicate divided into 2 haves. Half collected in serological tubes containing a pinch of lithium heparin powder, shaken gently and kept at 4ºC to test hematological parameters. Other half collected in tubes without of anticoagulant and allowed to clot at 4ºC for 2hrs to test serological parameters. The clot was the spun down at 2000g for 10 min to separate the serum. The serum collected by micropipette and was stored in sterile Eppendorf tubes at -20ºC until used for assay. Blood samples for measurement of hematological parameters including red blood cell count (RBC) and white blood cell count (WBC), differential cell counts (monocytes, lymphocytes and neutrophiles), and blood indices, mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), percentage of hemoglobin (Hb) and hematocrit (Hct), and also blood biochemical parameters including serum total protein, albumin, globulin and also immunological parameters such as lysozyme activity, phagocytic activity and respiratory burst activity were done. The results showed that there were not significant differences in hematological parameters between experimental groups with placebo group in identical weeks (p〉0.05), but there were significant differences in biochemical parameters and immunological parameters between experimental groups with placebo group in identical weeks (p〈0.05). Levels of serum total protein, albumin, and globulin and also lysozyme activity, phagocytic activity and respiratory burst activity were higher in experimental groups than placebo group in identical weeks. Immunology indicators of changes were compared between the two groups of fish 13 g and 2 g at the end of the eighth week after feeding. The results were indicated that the response of the respiratory burst activity; phagocytic activity and serum lysozyme activity were higher in juveniles of 13 gram than juveniles 2 gram. In conclusion, dried Origanum Vulgare and Aloe vera extracts at a rate of 1% of weight feed increased specific and non-specific immune systems in rainbow trout (13 and 2 gram) in identical weeks (2, 4, 6, 8 and 10). Therefore, these extracts can use to enhance immune system in aquaculture industry.
    Keywords: Aquaculture ; Health ; Iran ; Immunestimulators ; Iranian Medicinal Plants ; Fish ; Respiratory burst activity ; Lysozyme ; Phagocytosis ; Neutrophil ; Macrophage ; Total protein ; Origanum vulgare L. ; Aloe vera ; Rainbow trout ; Oncorhynchus mykiss
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  • 94
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    Hydrobiological studies on selected creeks in east Hormozgan for shrimp culture pond purpose (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25223 | 18721 | 2018-09-06 05:59:09 | 25223 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The coastal ecosystem, which represents the boundary in between pelagic province and land, is a complex one. Since most probes of physico-chemical characteristic in coastal ecosystem are unstable, it affects the distribution and abundance of the biota in it. The aim of this project was to determine Hydro-biological studies on the important creeks of East Hormozgan for shrimp culture pond purpose. Three creeks was selected on east coast namely as (Azini, Jask and Khalasi) and three stations in every creek which situated at the mouth, middle and end of the creek. The work in the project is incorporated in living and nonliving characteristics; the sampling period was in four seasons of 1384. Water characteristic like temperature, pH, dissolved oxygen, biochemical oxygen demand (B.O.D), salinity, carbon dioxide(Co2), nitrate, nitrite, ammonia, phosphate, silicate, total dissolved solid (T.D.S), total suspended particle,(T.S.S) and hydrogen sulphide (H2s). The recorded mean of these parameters in Azini creek (25.5±1.05 c), (8.11±0.13), (7.21±0.82 mg/l), (2.3±0.73 mg/l), (38.07± 0.86 ppt), (12.4±1.8 mg/l), (3.12±2.8 μg/l), (0.27±0.19 μg/l), (1.39±0.99 μg/l), (0.27±0.22 μg/l),(9.78±5.36 μg/l), (28.2±2.1 g/l), (37.6±11 mg/l),(0.1±0.07 mg/l), Jask creek (27.5±1.1 c ), (7.96±0.33), (6.79±0.77 mg/l), (2.9±0.96 mg/l), (38.77± 1.68 ppt), (10.8±3.4 mg/l), (3.51±2.2μg/l), (0.2±0.15 μg/l), (2.13±1.48 μg/l), (0.55±0.42 μg/l), (13±6.3 μg/l), (38.8±1.9 g/l), (37.1±13 mg/l),(0.32±0.28 mg/l), and Khalasi creek (28.2±1.9 c), (8.04±0.16), (6.9±0.48 mg/l), (2.22±1.03 mg/l), (38.79± 1.46 ppt), (12 ±1.1 mg/l), (3.08±1.4 μg/l), (0.2±0.14 μg/l), (1.41±0.65 μg/l), (0.45±0.39 μg/l), (13.3±4.2 μg/l), (38±3.5 g/l), (35.3±9 mg/l),(0.08±0.04 mg/l), respectively. The range of Total Organic Matter and Total Phosphor of sediment in Azini creek recorded as (4.99-10.57), (0.4-1.73), Jask creek (2.57-5.89), (0.0-1.99) and Khalasi creek (5.23-6.89), (0.59-1.09) respectively. Comparison of concentration mean between three creek shows there was significant difference between Temperature , pH, Dissolved Oxygen, Salinity, free Carbon dioxide, Silicate, Phytoplankton and Zooplankton (P〈0.05. Phytoplankton with (31 genera from 3 families),(34genera from 4 families) and (38 genera from 3 families) were recorded in Azini, Jask and Khalasi creeks respectively. Zooplankton with 12, 12, and 10 group was present in Azini, Jask and Khalasi creeks. Macrofauna with (54 species from 38 families and 20 Order), (35species from 25 families and 17 Order) and (51 149 species from 39 families and 20 Order) were recorded from Azini, Jask and Khalasi creeks respectively. Minimum and Maximum density of phytoplankton in Azini, Jask and Khalasi creeks were (63-22914), (68-94320) and (280-5523) in liter, Zooplankton (193048-226337), (36750-713753) and (32625-489532) in m^3 and Macrofauna (6400-11357), (8375-24800), and (900-12473) in m^2, Total Count (90-3400), (70-22150), and (0-2250) in one gram sediment and Total Vibrio (43-2400), (15-2400) and (25-460) in one gram sediment respectively.
    Keywords: Ecology ; Iran ; Hormozgan province ; Hydrobiology ; Creek ; Shrimp ; Culture Pond ; Ecosystem ; Abundance ; Temperature ; pH ; Dissolved oxygen ; Biochemical oxygen ; Salinity ; Nitrate ; Nitrite ; Ammonia ; Phytoplankton ; Zooplankton ; Macrofauna ; Sediment
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  • 95
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    A study on the diversity and abundance of ichthyoplankton in coastal waters of the Kharg and Dylam (Persian Gulf) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25221 | 18721 | 2018-09-06 06:08:35 | 25221 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The present investigation was undertaken to identify fish larva and estimate their abundance, diversity and evenness indices. Twelve stations were sampled monthly during daytime hours by Bongonet in the kharg and khargoo regions of Persian Gulf from January 2005 through December 2006. Forty family of fish larva were identified, Families of Silaginidae, Clupeoidea, Blenidae and Athrinidae consisted of more than 61 percent of all specimen; The abundance of specimen were more during spring through fall months and least during winter. The number and abundance of identified families varied from cruise to cruise and station to station; the average amount of diversity, Species Richness and evenness in investigated region were computed 1.93, 12 and 0.68, respectively. Temperature and salinity of water during this study were between 16-34°C and 35-42 gr/lit. There weren’t any significant correlation between fish larva indices and above factors.
    Keywords: Biology ; Iran ; Persian Gulf ; Kharg ; Dylam ; Diversity ; Abundance ; Ichtyoplankton ; Fish larvae ; Sample ; Silaginidae ; Clupeoidea ; Blenidae ; Athrinidae ; Specimen ; Species ; Temperature ; Salinity ; Coastal waters
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  • 96
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    Experiments on polyculture of pike, Esox lucius, with carps (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25235 | 18721 | 2018-09-07 06:41:04 | 25235 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The Goal of project implementation was survey of biological roles of Northern pike, Esox lucius, in decreasing of Coarse fish and other un economics organisms in carps ponds and also increasing of final crops in hectare and determination of Suitable ration of pike in ponds. This project implemented by 5 treatment with 3 replicates for every ones in 2 years. The pike density was 200, 350, 500 and 650 fish individuals per hectare for 1, 2, 3 and 4 treatment, respectively, and fifth treatment was without pike. The treatments of 1 and 3 conducted in first year and other in second year of test period. At first, about 26 brooders Caught from Anzali Lagoon, and reproduced by artificial and semi - natural methods in Sefidroud Fisheries Research Station in 12–14 °c temperature. Produced larvae reared in earthen ponds and fries reached to weight of 10 -12 g after 45 days. Mean weight, length and survival rates of fish was 12.27 and 32.5 g, 11.44 and 15.83 cm, and 40 and 21% after 45 and 60 days, respectively. The carp density in ponds was 3500 fish individuals per hectare and stocking rates were 55 % silver carp, 20 % common carp, 10 % bighead carp and 15 % grass carp. In throughout of the experiment period, physico– chemical factors measured and plankton (phyto and zoo), benthos, un wanted fish and other organisms investigated. The biomass of unwanted fishes reduced to 76/81 and 60/6 % in first and second year of experiments respectively. The results showed that Esox lucius had effect in increasing of objective fish produce about 17.9 % and 3.9 % in first and second period of project implementation respectively. The average of fish produce in pond unit (2400 m^2)was 842 kg (3508 kg/ha ), 825 kg (3439 kg/ha) and 776 kg (3232 kg/ha) in first , second and control treatments first year of test period , and also in second year was 865 kg (3603 kg/ha), 877 kg (3652 kg/ha) and 848 kg (3531 kg/ha) in second , fourth and control treatments , respectively. A significant different found in final weight mean of common carp between treatments and Control ponds (P 〈0/05), as the weight of common carp in treatments ponds was 220 % in first year and 191 % in second year more than control pond. In end of the trial, the mean weight of Esox lucius was 265 ± 74, 276 ± 104, 159 ± 33 and 265 ± 89 g in 1, 2, 3 and 4 treatment, respectively. Survival rate of Northern pike in 1 to 4 treatment was 43.8, 55.2, 40 and 35.6 %, respectively.
    Keywords: Aquaculture ; Iran ; Sefidroud River ; Polyculture ; Pike ; Esox lucius ; Carp ; Survey ; Organisms ; Density ; Temperature ; Larvae ; Earthen ponds ; Plankton ; Survival rate
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  • 97
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    Studying the impacts of the rainbow trout farms effluents on Sabzkooh River in Charmahal-O-Bakhtiari Province (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25238 | 18721 | 2018-09-07 06:48:16 | 25238 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Sabzkooh in Ardal and Gandoman region in Charmahal province with annual production of 1300 metric tons of rainbow trout, is one of the most important rivers in the province. The effects of fish farms effluent on the river water quality and the possibilities to develop or limit the existing ones were studied. Flora and fauna of the river including Phyto and zooplankton, macroinvertebrates and fishes were sampled and studied. Moreover some key physico-chemical water quality parameters, concentrations of stable pollutants (heavy metals: Zn, Cu, Fe, Pb, Cd and agricultural poisons (herbicides or pesticides: DDT, Lindane, Alderine and indosulfate) and Coliform contamination were also measured. All phytoplankton genus were of tow taxa, Cianophyta and Chrysophyta and zooplankton fauna belonged to Rotatoria, Protozoa and meroplanktonic chirinomids and nematodes. Four fish species from 3 families were identified of which rainbow trout had the highest relative frequency (94.6%).All the measured heavy metals including Zn, Cu, Fe, Pb, Cd and the agricultural poisons concentrations such as DDT, Lindane, Alderine and indosulfate were lower than the hazardous level suggested for the surface waters. According to the benthic macroinvertebrates populations study, stations at downstream (no. 6 and 7) which received the Chartakhteh and Rudarud farms effluent had least EPT richness and the hilsenhof family level biotic index at this locations was greater than 5.25, classifying them as having organic pollution. Even though measured key physico-chemical parameters such as nutrients (N and P compounds and BOD5) in the receiving stations and effluent water never exceed the maximum permitted range but in downstream stations these factors were very close to the range. One way analyze of variance (P 95%) for TN, TP, No_2, NH_4, BOD_5, pH, PO_4 and Ec revealed no significant differences between stations before and just after receiving the effluents, however these parameters in the effluents themselves significantly differ from upstream stations 1,2 and 3 proving relatively lower organic pollution and the potential for constructing new farms or expanding the existed ones in upstream region from Chartagh to Dehnoo. At downstream from Dehnoo to Rudarud and lower parts, dense trout farm effluents in addition with point and non point pollution (municipal, agricultural and other coming run off from the catchments area) had remarkable effects on the river water quality and as a result no farm construction or expansion is recommended. To avoid or reduce the existing farms effects on the river system there should be certainly some remediation actions carried out. Constructing sedimentation ponds before releasing the effluents, obeying the rule of water extracting, preventing fish escape, increasing feeding efficiency and controlling the lateral inlets from catchments area which are potentially pollutant, should be considered to improve the situation and prevent further environmental problems caused by fish farms effluent in these area.
    Keywords: Aquaculture ; Iran ; Charmahal Bakhtiari Province ; Rainbow trout ; Effluent ; Pollution ; Fauna ; Phytoplankton ; Zooplankton ; Heavy metals ; Protozoa ; Lindane ; Alderine ; Indosulfate
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  • 98
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    Study on biotic and abiotic parameters to determine water quality of Rajaei Dam (Mazandaran province- Sari) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25655 | 18721 | 2018-10-14 02:43:22 | 25655 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The The Shahid Rajaei Reservoir- Sari is an important and large reservoir in Iran. The major objectives of dam instruction are flood control, irrigation purposes, and electricity power. However the dam is going to supply drinking water for the people. In order to prevent threats of unsuitable water to human health risks and economic losses, it is necessary to monitor the water quality before offering it to people. In this study, some of the physicochemical parameters and Chlorophyl- a, phytoplankton, microb and fungi of Shahid Rajaei reservoir were measured at 4 stations (Shirin Roud branch, Sefid Roud branch, the crossing point of branches, near the tower) during six sampling months (June, July, August, September, November and February) in 2012-2013. In order to water quality classification, the water quality index, trophic status, Shanoon and saproby indices of reservoir calculated and the results compared to different criteria and standards. The results showed that the mean (±Standard Error) of temperature, dissolved oxygen, pH, phosohate, amonium and nitrate concentrations and Chlorophyl a were 21.35 (±1.30)ºC, 10.48 (±0.37), 8.54 (±0.04), 0.050 (±0.004), 0.036 (±0.004), 0.75 (±0.03) mg/l and 18.00 (±7.23) mg/m^3, respectively. In the present study, temperature between surface and deep layer was stratified in June and July, which the stratification was registerd 0.47 and 0.69 °C decreases with increasing of each meter depth in 15 to 30 meter culumn. But, these changes for each increasing meter of water depth were 0.2 to 0.26 °C in August and September, respectively, and finally was close to zero in November. In the warm months (July, August and September) with the formation of thermal stratification in the reservoir was formed oxygen stratification, but in the cold season (November and February), with vertical mixing of water oxygen and percent saturation of the reservoir was nearly homogeneous. TSI showed the maximum and minimum values at stations 4 (oligotrophic condition) and 2 (mesotrophic condition), respectively. The maximum and minimum monthly values of TSI obtained in July, August (eutrophic level) and September, February (oligotrophic level) respectively. Based on the Water Quality Index (WQI), the reservoir was in the “good” quality in whole months, .This class shows that the reservoir is suitable as source of drinking water through routine treatment of drinking water and the quality of water is rarely is low. Meanwhile the water is suitable for swimming and water recreation and survives of .sensitive fish and other aquatic species. Based on the results, 107 phytoplankton species were identified during the period of study. The species were classified in 8 divisions. Maximum and minimum values of mean (SE) abundance observed in July and January, 661 (±286) and 10 (±2) million cells/m^3 respectively at the surface layer. The One way analysis of abundance and biomass data showed temporal significant variances (P〈 0/05), however the spatial variances of data were not significant (P〉 0/05). Bacillariophyta and Pyrrophyta formed more than 95% of phytoplankton. 3 dominant species namely, Cyclotella meneghiniana, Goniaulax polyedra and Ceratium hirundinella formed about 70% of phytoplankton aboundance. Comparison of diversity indices (Shannon and Evenness) showed higher values in May and January; however the indices reached its lowest level (0.58 and 0.16) in August. Water quality assessment using Shannon index showed the lowest quality of water (moderately to high polluted) in July and August. This index demonstrated the highest water quality (slightly polluted) at station 1 and 4 respectively. The results of the water quality assessment using Saproby index (based on the resistant phytoplankton species to organic pollution) also indicated to organic pollution of water in the months of summer. The saproby assessment in stations categorized most of the stations in “moderately polluted” class of organic pollution except at station 4 which was in "slightly polluted" class. In conclusion, the removal (transfer) place and time of water to the water treatment plants.are impratnt because of temporal and spatial variation of water quality due to changes of phytoplankton structure in Shahid Rajaee Reservoir. Meanwhile, the survey showed that physico-chemical parameters alone did not reflect the actual conditions of aquatic water bodies. Monitoring of aquatic ecosystems must be complemented by biological monitoring. Microbial survey showed that the maximum and minimum geometric mean of the total number of bacteria, in September (6101559 CFU/100ml) and February (3310 CFU/100ml) respectively. However in stations, the maximum and minimum count of this parameter obtained at stations 2 (455316 CFU/100ml) and 3 (40964 CFU/100ml) respectively. There were no viable count of total coliform in the months of May and June. However it’s counting reach to the maximum value in September. Clostridium perfrigens showed viable count in water sample during September. The results also showed no proportion of fecal streptococci in microbe account in the Shahid Rajaei Reservoir. It might be good sign of suitable water quality in term of no-contamination by old and resistant fecal microbes. Base on the total coliform count, water quality was suitable for swimming and source of drinking water in most stations and months. The coliform count increased in August and September in stations 3 and 4. In these 2 months the probability of new fecal contamination increased by warm blood animals in the reservoir. It seems that the environment in September is suitable for accession of old and resistant microbes such as Clostridium perfrigens. The results of sample analysis revealed that the fungal colony counts in the station 4 and 5 were significantly higher than those the other stations. Moreover, the minimum and maximum of the fungal colony counts wereobserved in August and February, respectively. The most commonly isolated genera were Aspergillus, yeasts (especially candida) Penicillium, Cladosporium, Mucor, Fusarium, Althernariya, sterile hyafe and Paecilomyces respectively. Finally, in order to prevent of occurrence of eutrophication, algal bloom, and control of microbial activities and organic phosphorus loading it is necessary to control the activities of the human societies around the dam or the rivers tributary.
    Keywords: Ecology ; Iran ; Mazandaran Province ; Sari ; Shahid Rajaei Reservoir ; Water Quality ; Trophic State ; Biotic ; Abiotic ; Chlorophyll-a ; Phytoplankton ; Microbe ; Fungi ; Temperature ; Dissolved oxygen ; pH ; Aquatic ; Species
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  • 99
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    Investigate effects of temperature, salinity, light duration, density and cannibalism on growth and survival of tilapia larvae in brackish water, Bafq (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25669 | 18721 | 2018-10-08 08:35:41 | 25669 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: This project was conducted in five independent experiments in appropriate tanks with suitable aeration and water fellow. Temperature: an experiment designed to evaluate the effect of temperature on growth and survival of tilapia fry by using four thermal regimes consist of 22, 25, 28 and 31 °c in three replicates. Fries with initial wight of 0.14 g were stocked in plastic container with 10 liter capacity at the rare of 5/liter. Fish were fed on rainbow trout commercial food at a rate of 30 % of biomass 5 times per day. The results showed that some growth indices such as final body weight, daily growth rate, specific growth rate and weight gain increased by increasing water temperature significantly. Although fry survival increased by increasing temperature but these differnces were not significant. the results suggest that in larviculture of Nile tilapia water temperatue should not be less than 28°c. Density: compressibility of Nile tilapia fry was studied by using plastic container with 13 liter capacity. Fry with initial weigh of .034 g were stocked in four treatments 10, 15, 20 and 30 fry/l with four replicates. They fed on rainbow trout food according to their biomass five times per day during the light period. The results showed that some growth indices such as average of final body weight, daily growth rate, specific growth rate and survival decreased by increasing stocking density significantly, while feed conversion rate increased by increasing stocking density. Considering the experiment, it could be suggested that lower stocking density (10/l) resulted the best growth efficiency and survival of Nile tilapia fry. On the other hand, compering the results of growth and survival rate(84%) in two stocking densites(15 and 20/l) showed that it is passible to culture Nile tilapia fry at the stocking density of 20fry/l in suitable condition. Salinity: A study conducted to evaluate the effect of salinity on growth and survival rate of Nile tilapia fry by using six salinity levels (0, 4, 8, 12, 16 and 20 ppt) with three replicates. Fry fed on rainbow trout food five times per day during the light period. Results showed that some growth indices such as average of final body weight, daily growth rate, specific growth rate and survival rate increased by increasing salinity levels up to 8 ppt significantly, and then decreased by increasing salinity levels up to 20 ppt. according to the experiment, it seems that larviculture of Nile tilapia in brackish water is available and the best results achives at 8 ppt. considering the results of growth and survival rate at 16 ppt showed that Salinity up to 16 ppt was tolerable, although reduced the growth and survival of Nile tilapia fry. Photoperiod: the objective of this study was to examine the effects of photoperiod on growth and survival rate of Nile tilapia fry by planning four treatments (6L:18D, 12L:12D, 18L:6D and 24L:0D) and four replicates. Light provided by tow fluorescent lamps those set at a distance of 60 cm above the tanks and worked by an automatic timer. Fry fed on rainbow trout food five times per day. Results showed that average of final body weight, daily growth rate and specific growth rate were loest in 6L:18D and these indices increased by increasing light duration. these differences were not significant among 6L:18D and 12L:12D. but the differences between 6L:18D with 18L:6D and 24L:0D were significant. Evaluation of survival rate showed that photoperiod did not significant effect on survival in all treatments. These finding suggests that a 12L:12D cycle be adequate in case of larval rearing. Cannibalism: An experiment designed to determine the occurrence of cannibalism among 5 different size groups of (5, 10, 20 and 30 g with fry 0.45 g) Nile tilapia population (fry / fingerling) under two stocking densities (1 / 2 and 1 / 4 fingerling / fry) with three replicates in the poly etilen tanks in brackish water condition. Fry were counted at 6, 24, 48, 72, 96, 120 and 144 hours after stocking in two methods (feeding and no feeding). In the feeding trial fingerling were fed on rainbow trout food two times per day. The results showed that cannibalism became more intense as the size difference increased. After passing time cannibalism rate increased. Also results showed increasing fry density causes increasing cannibalism in both treatments (feeding and without feeding). Feeding fingerlings (predator) was effective in reducing cannibalism.
    Keywords: Aquaculture ; Iran ; Bafq ; Oreochromis niloticus ; Fry ; Temperature ; Stocking density ; Salinity ; Photo period ; Cannibalism ; Brackish Water ; Growth ; Survival ; Tilapia ; Larvae
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  • 100
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    Investigation of situation of propagation and culture of rainbow trout in north of Alborz (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25711 | 18721 | 2018-10-10 09:44:09 | 25711 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The aquaculture activity in provinces of north of Iran, in spite of fast progressive and possess a series of achievement and success, there was some failure, difficulties, limitation and restriction. To planning for further expansion in this failed, this study is managed. The culture of rainbow trout is practice in individual farms, small farms, water resources, earthen ponds, recycling ponds etc, which each method have its potential, failure and limitation that are consider in the present work. At the end, based on the situation of production in past years, and considering each of factors mentioned above, the success, failure, difficulties, limitation and restriction points of aquaculture of rainbow trout in technical poin of view are investigated and some command for improvement for each production method are presented. The development of aquaculture in recent years is based on extension of farms and increasing density of fish in fish pond. In contrast of many production activities, the propagation and culture of fish is inconstant work. This variability makes a potential for better income, occupation and job, increasing in production etc. This study make a better planning using this activity.
    Keywords: Aquaculture ; Iran ; Alborz ; Rainbow trout ; Water resources ; Fish culture ; Aquaculture ; Investigation ; Propagation
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 66
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