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  • 1
    Publication Date: 2021-05-19
    Description: Study of survey health management and diseases in hatcheries and fish farms can help us to knowledge and application control methods such as: prevention, treatment and increase high levels of production in hatchery and farms, finally. This survey carried out from 2005 to 2008 for 4 years in sturgeon hatcheries and farms of Golestan province. Sturgeon fishes include Huso Huso, Ship sturgeon, Acipenser persicus collected and for virology, bacteriology, fungius and hematology examined. Also, physicochemical parameters measured and recorded in different stages of culture. Results of this study showed that all of samples in virology was negative and did not observe any doubetful causes. In bacteriology CFU was variation from 3/9 ×105 to 6/9×10. The most parasites that detected in this survey was Cocolanus espherolanus , Sceria binopsulus semiarmatus and Amphilina fuliacea that separates from Acipenser Percicus, especially. The results about hematology parameters some important hematological indices of ship sturgeon include: The total RBC for female and mail specimens measured as 5.3±1.5 ×105, 4.8±0.5 ×105 per mm 3 respectively. The amount of haematocrit and hemoglobin for female and mail determined: 34.3±2.8, 35±1.4 percent and 10.3±0.9, 8.9±0.8 gr/dl .The MCV: 216.3± 96.2, 736.5± 102.5, MCH: 720.2±309.5, 186±0.7 and MCHC: 30±0.8, 25.5±3.4 percent respectively.The total WBC were (female, male): 21320±1054, 20580±777 per mm 3 and neutrophil: 16.4±2.5, 17±1.4 percent and lymphocyte: 74.4±2.4, 73.5± 0.7 percent and eosinophil: 6±1.4, 6.4±0.5 percent, monocyte: 2.8±0.8, 3.5±0.7 percent. There was not any significant differences (p〉0.05) between mentioned parameters in male and female (students t-test). Also evaluation of hematological parameters in bluga ( Huso huso) include: total RBC were (male , female) 5±0.3 ×105 , 4.9±0.6 ×105 per mm 3 ,respectively and hematocrit: 33.2±6.7 , 35.4±3.4 percent and hemoglobin: 11.2±1.5 , 12.2±1gr/dl and MCV: 669.9±172.2, 723.9±982.4 and MCH: 226.2±42.5, 249.5±35.4 and MCHC: 34.1±2.4, 34.6±3.6 percent respectively. The totals WBC were (male, female): 24800±707.1, 23042±1375.4 per mm 3 and neutrophil: 18.5±0.7, 21.4±1.1 percent and lymphocyte: 73.5±1.4, 68.4±1.1 percent and eosinophil: 5±2.8, 7±1.2 percent and monocyte: 3.5±3.5, 3.2±0.8 percent. According to statistically study the count of lymphocyte had significant difference between male and female fish and this count in male was higher than female. (p≥0.05).
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Physicochemical ; Hematology ; Fish ; Sturgeon ; Huso huso ; Ship ; Acipenser percicus ; Bacteriology ; Parasitology ; Health management ; Diseases ; Survey ; Aquaculture ; Hatcheries ; Samples ; Sceria binopsulus ; Amphilina fuliacea ; Female ; Male
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 395pp.
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  • 2
    Publication Date: 2021-05-19
    Description: Nisin is a natural antimicrobial and has inhibitory effect on the pathogens and spoilage organisms. The efficiency of nisin reduced after reaction with food compounds. There is the hypothesis that combination of nisin with organic salt increases the shelf life of fish. The purpose of this study was mainly to evaluate the effects of nisin (0.15 %) and sodium acetate ( 1% ) individually and in combination on shelf life of fillets and whole trout ( without viscera). then, the behavior of Listeria monocytogenes and Clostridium botulinum type E were evaluated in different time at 40C. Physical and chemical parameters including: pH, Peroxide Value (PV), Tiobarbithoric Acid (TBA) and Total Volatile Nitrogen (TVN) and bacterial factors such as Total Viable Count (TVC), Pscycrothophic Count (PTC) and Lactic Acid Bacteria (LAB) were examined. These parameters were done at intervals of 4 days for 16 days. Results showed that chemical and bacterial factors of whole trout (Oncorhynchus mykiss) ( (without viscera) have been more favorable than the fillets . It also showed that the peroxide value , the thiobarbituric acid, volatile nitrogen bases , and pH in combination of sodium acetate and nisin Z treatments were exposed to significantly compared to control treatments ( no preservatives ) (P〈0.05). Treatments with sodium acetate and nisin Z (individual ) were exist after combination treatment. Hence it can be concluded that the maximum shelf life of in preservative treatments was 16 days , but control treatment was 12 days in some cases and in some cases 16 days. Effects of treatments on Listeria monocytogenes and Clostridium botulinum in the control treatment showed the highest growth was observed in both species ( log 8 ) but in single and combined treatment of bacterial growth slower , but is still the trend the 16th day of so Listeria and Clostridium log 5 to log 6 , respectively. Vegetative cells of Clostridium were more sensitive in compare to Listeria. When using preservatives (chemical or biological) in fish tissue, parameters such as the type of preservative, used method, pH, proteolytic enzymes, the incidence of resistant strains and so on influence of antimicrobial agents used. The overall conclusion of the study was shelf life of whole trout (without viscera) was more than fillets, combination of nisin and sodium acetate were better than other treatment and finally higher doses of bilogival preservatives are need to for achieve to significant reduction of pathogenic bacteria .
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Nisin Z ; Sodium acetate ; Rainbow trout ; Listeria ; Monocytogenes ; Clostridium botulinum ; Lactic Acid ; Bacteria ; Organic acids ; Oncorhynchus mykiss ; Pathogen
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 94pp.
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  • 3
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25451 | 18721 | 2018-09-23 17:53:51 | 25451 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Nowadays, medical plants extract is used in aquaculture industry to enhance growth, immune system and fish resistance to different pathogens. In this study, some non-specific immune responses, hematology and biochemical parameters in rainbow trout juveniles (16 gr mean weight) and their resistance to Streptococcus iniae were evaluated following oral administration of Echinacea purpurea and Astragalus sp. extract. Three concentrations (0.5, 1 and 1.5 gr/kg of feed) of Echinacea purpurea and three concentrations (2, 3 and 5 gr/kg of feed) of Astragalus sp extract and control group were used for 60 days. Parameters evaluated included: levels of C4, C3 (complement components), free oxygen radicals (respiratory burst), Lysozyme, numbers of lymphocytes, monocytes and neutrophils. In the end, the relative survival rate (RSR) of fish was evaluated against S. iniae. The results showed that the levels of C3, lysozyme, oxygen free radicals, the number of neutrophils and lymphocytes in the experiment groups (the highest dose) compared to control group were increased significantly (p 〈0/05). While, the values of C4 and number of monocytes compared to the control group were not significantly different (p〉 0.05). The relative survival rates of fish following challenge with Streptococcus iniae, were 91/11, 93/33 and 44/44 percent in experiments (Echinacea purpurea and Astragalus sp.) and control groups, respectively. In conclusion, it may be state that Echinacea purpurea and Astragalus sp. extracts enhance the non-specific immune system and fish resistance against Streptococcusis, and it seems it can be used as immunostimulant in feed.
    Keywords: Aquaculture ; Iran ; Echinacea purpurea ; Astragalus sp ; Rainbow trout ; Streptococcusis ; Immune system ; Astragalus glycyphyllos ; O.mykiss ; Aquaculture ; Growth ; Juvenile ; Lysozyme ; Survival rate
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 106
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  • 4
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    Unknown
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25330 | 18721 | 2018-09-13 12:40:49 | 25330 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Study of survey health management and diseases in hatcheries and fish farms can help us to knowledge and application control methods such as: prevention, treatment and increase high levels of production in hatchery and farms, finally. This survey carried out from 2005 to 2008 for 4 years in sturgeon hatcheries and farms of Golestan province. Sturgeon fishes include Huso Huso, Ship sturgeon, Acipenser persicus collected and for virology, bacteriology, fungius and hematology examined. Also, physicochemical parameters measured and recorded in different stages of culture. Results of this study showed that all of samples in virology was negative and did not observe any doubetful causes. In bacteriology CFU was variation from 3/9 ×105 to 6/9×10. The most parasites that detected in this survey was Cocolanus espherolanus, Sceria binopsulus semiarmatus and Amphilina fuliacea that separates from Acipenser Percicus, especially. The results about hematology parameters some important hematological indices of ship sturgeon include: The total RBC for female and mail specimens measured as 5.3±1.5 ×10^5, 4.8±0.5×10^5 per mm^3 respectively. The amount of haematocrit and hemoglobin for female and mail determined: 34.3±2.8, 35±1.4 percent and 10.3±0.9, 8.9±0.8 gr/dl .The MCV: 216.3± 96.2, 736.5± 102.5, MCH: 720.2±309.5, 186±0.7 and MCHC: 30±0.8, 25.5±3.4 percent respectively.The total WBC were (female, male): 21320±1054, 20580±777 per mm^3 and neutrophil: 16.4±2.5, 17±1.4 percent and lymphocyte: 74.4±2.4, 73.5± 0.7 percent and eosinophil: 6±1.4, 6.4±0.5 percent, monocyte: 2.8±0.8, 3.5±0.7 percent. There was not any significant differences (p〉0.05) between mentioned parameters in male and female (students t-test). Also evaluation of hematological parameters in bluga ( Huso huso) include: total RBC were (male , female) 5±0.3 ×105 , 4.9±0.6 ×105 per mm^3 ,respectively and hematocrit: 33.2±6.7 , 35.4±3.4 percent and hemoglobin: 11.2±1.5 , 12.2±1gr/dl and MCV: 669.9±172.2, 723.9±982.4 and MCH: 226.2±42.5, 249.5±35.4 and MCHC: 34.1±2.4, 34.6±3.6 percent respectively. The totals WBC were (male, female): 24800±707.1, 23042±1375.4 per mm^3 and neutrophil: 18.5±0.7, 21.4±1.1 percent and lymphocyte: 73.5±1.4, 68.4±1.1 percent and eosinophil: 5±2.8, 7±1.2 percent and monocyte: 3.5±3.5, 3.2±0.8 percent. According to statistically study the count of lymphocyte had significant difference between male and female fish and this count in male was higher than female. (p≥0.05).
    Keywords: Aquaculture ; Iran ; Golestan Province ; Mazandaran Province ; Guilan Province ; Fish ; Sturgeon ; Huso huso ; Ship ; Acipenser percicus ; Bacteriology ; Parasitology ; Health management ; Diseases ; Survey ; Aquaculture ; Hatcheries ; Samples ; Sceria binopsulus ; Amphilina fuliacea ; Female ; Male
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 395
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  • 5
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25650 | 18721 | 2018-10-14 02:41:46 | 25650 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Nisin is a natural antimicrobial and has inhibitory effect on the pathogens and spoilage organisms. The efficiency of nisin reduced after reaction with food compounds. There is the hypothesis that combination of nisin with organic salt increases the shelf life of fish. The purpose of this study was mainly to evaluate the effects of nisin (0.15 %) and sodium acetate ( 1% ) individually and in combination on shelf life of fillets and whole trout ( without viscera). then, the behavior of Listeria monocytogenes and Clostridium botulinum type E were evaluated in different time at 40C. Physical and chemical parameters including: pH, Peroxide Value (PV), Tiobarbithoric Acid (TBA) and Total Volatile Nitrogen (TVN) and bacterial factors such as Total Viable Count (TVC), Pscycrothophic Count (PTC) and Lactic Acid Bacteria (LAB) were examined. These parameters were done at intervals of 4 days for 16 days. Results showed that chemical and bacterial factors of whole trout (Oncorhynchus mykiss) (without viscera) have been more favorable than the fillets . It also showed that the peroxide value, the thiobarbituric acid, volatile nitrogen bases, and pH in combination of sodium acetate and nisin Z treatments were exposed to significantly compared to control treatments (no preservatives) (P〈0.05). Treatments with sodium acetate and nisin Z (individual) were exist after combination treatment. Hence it can be concluded that the maximum shelf life of in preservative treatments was 16 days, but control treatment was 12 days in some cases and in some cases 16 days. Effects of treatments on Listeria monocytogenes and Clostridium botulinum in the control treatment showed the highest growth was observed in both species (log 8) but in single and combined treatment of bacterial growth slower , but is still the trend the 16th day of so Listeria and Clostridium log 5 to log 6, respectively. Vegetative cells of Clostridium were more sensitive in compare to Listeria. When using preservatives (chemical or biological) in fish tissue, parameters such as the type of preservative, used method, pH, proteolytic enzymes, and the incidence of resistant strains and so on influence of antimicrobial agents used. The overall conclusion of the study was shelf life of whole trout (without viscera) was more than fillets, combination of nisin and sodium acetate were better than other treatment and finally higher doses of bilogival preservatives are need to for achieve to significant reduction of pathogenic bacteria.
    Keywords: Fisheries ; Iran ; Nisin Z ; Sodium acetate ; Rainbow trout ; Listeria ; Monocytogenes ; Clostridium botulinum ; Lactic Acid ; Bacteria ; Organic acids ; Oncorhynchus mykiss ; Pathogen
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 94
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  • 6
    Publication Date: 2021-07-05
    Description: In this study, some non-specific immune responses and hematological parameters in rainbow trout juveniles (16g mean weight) and their resistance to Streptococcusis were investigated following dietary administration of 3 concentrations (0.5, 1, 1.5g/kg of feed) of Echinacea purpurea extract. The non-specific immune response and hematological parameter compared with control group for 60 days. Evaluated parameters included were of C3, C4, (complement components), superoxide ions (respiratory burst), lysozyme activity, number of WBC, percentage of blood lymphocytes, monocytes and neutrophils. At the end of trial, the relative survival rate (RSR) of fish was evaluated against S. iniae. The results showed that the levels of C3, lysozyme activity, superoxide ions, number of WBC and percentage of neutrophils in the experiment groups (the highest concentration, 1.5g/kg of feed) were increased significantly compared to the control group. Whereas, no significant difference was found in the value of C4 and the percentage of monocytes and lymphocytes in comparison to the control group. The relative survival rates of fish following challenge with Streptococcus iniae, were 91.11 and 44.44 percent in experiment (Echinacea purpurea, concentration of 1.5g/kg of feed) and control group, respectively. In conclusion, the results of the present study demonstrated that Echinacea purpurea extract enhanced the non-specific immune system and fish resistance against streptococcusis, suggesting that this extract might be used as immunostimulant in fish feed.
    Keywords: Aquaculture ; Biology ; Chemistry ; Echinacea purpurea ; rainbow trout ; Streptococcusis ; immune system ; Oncorhynchus mykiss ; resistance ; weight ; Streptococcus iniae ; lysozyme ; fish feed ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 1-12
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  • 7
    Publication Date: 2021-05-19
    Description: Nowadays, medical plants extract is used in aquaculture industry to enhance growth, immune system and fish resistance to different pathogens. In this study, some non-specific immune responses, hematology and biochemical parameters in rainbow trout juveniles (16 gr mean weight) and their resistance to Streptococcus iniae were evaluated following oral administration of Echinacea purpurea and Astragalus sp. extract. Three concentrations (0.5, 1 and 1.5 gr/kg of feed) of Echinacea purpurea and three concentrations (2, 3 and 5 gr/kg of feed) of Astragalus sp extract and control group were used for 60 days. Parameters evaluated included: levels of C4, C3 (complement components), free oxygen radicals (respiratory burst), Lysozyme, numbers of lymphocytes, monocytes and neutrophils. In the end, the relative survival rate (RSR) of fish was evaluated against S. iniae. The results showed that the levels of C3, lysozyme, oxygen free radicals, the number of neutrophils and lymphocytes in the experiment groups (the highest dose) compared to control group were increased significantly (p 〈0/05). While, the values of C4 and number of monocytes compared to the control group were not significantly different (p〉 0.05). The relative survival rates of fish following challenge with Streptococcus iniae, were 91/11, 93/33 and 44/44 percent in experiments (Echinacea purpurea and Astragalus sp.) and control groups, respectively. In conclusion, it may be state that Echinacea purpurea and Astragalus sp. extracts enhance the non-specific immune system and fish resistance against Streptococcusis, and it seems it can be used as immunostimulant in feed.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Hematology ; Biochemical ; Echinacea purpurea ; Astragalus sp ; Rainbow trout ; Streptococcusis ; Immune system ; Astragalus glycyphyllos ; O.mykiss ; Aquaculture ; Growth ; Juvenile ; Lysozyme ; Survival rate
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 106pp.
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  • 8
    Publication Date: 2021-05-19
    Description: In this study, some non-specific immune responses and hematological parameters in rainbow trout juveniles (16g mean weight) and their resistance to Streptococcusis were invetigated following dietary administration of 3 concentrations (0.5, 1, 1.5g/kg of feed) of Echinacea purpurea extract. The non-pecific immune response and hematological parameter compared with control group for 60 days. Evaluated parameters included were of C3, C4, (complement components), superoxide ions (respiratory burst), lysozyme activity, number of WBC, percentage of blood lymphocytes, monocytes and neutrophils. At the end of trial, the relative survival rate (RSR) of fish was evaluated against S. iniae. The results showed that the levels of C3, lysozyme activity, superoxide ions, number of WBC and percentage of neutrophils in the experiment groups (the highest concentration, 1.5g/kg of feed) were increased significantly compared to the control group. Whereas, no significant difference was found in the value of C4 and the percentage of monocytes and lymphocytes in comparison to the control group. The relative survival rates of fish following challenge with Streptococcus iniae, were 91.11 and 44.44 percent in experiment (Echinacea purpurea, concentration of 1.5g/kg of feed) and control group, respectively. In conclusion, the results of the present study demonstrated that Echinacea purpurea. extract enhanced the non-specific immune system and fish resistance against streptococcusis, suggesting that this extract might be used as immunostimulant in fish feed.
    Description: Published
    Keywords: Echinacea purpurea ; Oncorhynchus mykiss ; Rainbow trout ; Streptococcusis ; Immune ; Fish
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.1-12
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