ALBERT

Description: No abstract available

Description: The 2010 Decadal survey failed to issue any recommendations on diversity and inclusion.Astro2020 cannot make the same mistake. Findings can be ignored by funding agencies;recommendations cannot. In the past decade, multiple groups have assembled detailed actionplans to fix a broken climate within our profession. Astro2020 should play a key role, bysynthesizing this work to produce actionable recommendations to support diversity andinclusion and stop harassment within our profession.

Description: Following Z-2 space suit testing that occurred from 2016-2017, the Exploration Extravehicular Mobility Unit (xEMU) Project was tasked with building a demonstration unit of the xEMU space suit to test on the International Space Station (ISS) in 2023. This suit is called xEMU Demonstration Suit (xEMU Demo). Based on feedback from astronauts during the Z-2 NBL test series, design changes were made, resulting in a new prototype suit called the Z-2.5 space suit. The design of the Z-2.5 space suit with an exploration Portable Life Support Systems (xPLSS) mock-up represents the architecture of xEMU Demo. The team is testing Z-2.5 in the NBL to evaluate this architecture and validate changes made from Z-2. The results will inform the xEMU Demo design going forward to its Preliminary Design Review (PDR) in the summer of 2019. This Z-2.5 NBL test series focuses on evaluating the microgravity performance of the suit and the ability to complete ISS-related tasks. The series is comprised of 10 manned runs and an unmanned corn-man run. Six test subjects, including four astronauts, will participate. The test objective is to evaluate ability xEMU Demo architecture to perform ISS microgravity tasks. Each crew members will complete both a familiarization run and a nominal EMU EVA timeline run. Qualitative and quantitative data will be collected to aid the assessment of the suit. Preliminary feedback from astronauts who have completed the test series evaluate the xEMU Demo architecture as acceptable to complete a demonstration mission on the ISS.

Description: For a variety of medical and scientific reasons, human bones can be exposed to ionizing radiation. At relatively high doses (30,0005,000 Gy), ex vivo ionizing radiation is commonly used to sterilize bone allografts. However, ionizing radiation in these applications has been shown to increase risk of fracture clinically and decrease bone quality. Previously, we observed a significant decrease in compressive static strength and fatigue life of ex vivo whole bones exposed to x-ray radiation at 17,000 Gy and above; no changes in compressive mechanical properties were observed for radiation doses of 1,000 Gy and below. The gap in doses between no mechanical change (1,000 Gy) and significant mechanical degradation (17,000 Gy) is large, and it is unclear at what dose mechanical integrity begins to diminish in whole bones, and if its effects differ in response to static versus cyclic mechanical loading. This is a major clinical concern, as trabecular and cortical bone allografts are commonly used in structural, load-bearing applications. To gain insight into the effect of ionizing radiation from 1,000-17,000 Gy, we conducted an ex vivo radiation study on the static and fatigue mechanical properties of the vertebral whole bone. Our objectives were to: (1) quantify the effect of exposure to ex vivo ionizing radiation on the mechanical integrity (compressive static and fatigue) of whole bones; and (2) evaluate, if there are observed differences in mechanics, if they differ in magnitude for static versus cyclic properties. The results of this study will give insight into the need for changes in protocols for bone allograft radiation sterilization procedures.

Description: No abstract available

Description: A spacecraft water disinfection system, suitable for extended length space exploration missions, should prevent or control the growth of microbes, prevent or limit biofilm formation, and prevent microbiologically-influenced corrosion. In addition, the system should have minimal maintenance requirements, the effluent should be chemically compatible with all materials in contact with the water, be safe for human consumption, and suitable to be shared across international spacecraft platforms and mission architectures. Silver ions are a proven broad spectrum biocide. Silver is also the potable water biocide of choice for future exploration missions. Currently, the proposed method for implementing silver biocide in spacecraft systems relies on silver electrode technology to produce a controlled amount of silver ions. Unfortunately, electrolytic-based silver dosing presents multiple inherent challenges that affect performance and increase maintenance requirements over time. To decrease the risk of failure, an alternative silver biocide delivery method is needed. Control-release technology is an attractive option for developing a passive high-reliability silver dosing device. The concept of a nanoparticle/polyurethane (PU) composite foam for the controlled release of silver was prompted by the controlled release technology developed by NASA for the delivery of corrosion inhibitors and indicators. This paper presents the technical background and results from the synthesis and properties testing of the silver nanoparticles (AgNPs)/PU composite foam that is being developed for use in spacecraft potable water systems.

Description: The development of engineering technologies and hardware for aerospace applications is often tracked on a 1-9 scale of readiness or TRL, with a "1" representing very basic or fundamental principles, and a "9" being flight tested, functional hardware. Preparing to grow crops for supplemental food and eventual life support contributions on space missions faces similar challenges. Nearly 20 years ago, the concept of a "crop readiness level" was suggested at a bioregenerative life support conference held at Kennedy Space Center, but there was little follow up to this. We propose to revive this concept to track the preparation and testing of different crop species for eventual use in the unique environment of space. For the sake of uniformity, we recommend a 1-9 scale, with a "1" being just the identification of a potential crop, followed by some basic horticultural testing, cultivars trials, then testing growth and yield under various controlled environments, progression to more space-like environments and hardware, understanding the nutritional, organoleptic, and food safety aspects of the crop, initial testing in space, and a final stage of growing the crop for food in space ("9"). We attempted to make the scaling logical and progressive, but our main goal is to initiate a dialogue in the space, plant research community to develop a scale for assessing crop readiness.

Description: This is our annual "station report" of activities related to controlled environment research to the North Central Education Research Activity (NCERA-101) committee. The committee is sponsored the USDA National Institute for Food and Agriculture (NIFA). Kennedy Space Center has participated in this committee for over 30 years.

Description: A well-known hazard associated with exposure to the space environment is the risk of vehicle failure due to an impact from a micrometeoroid and orbital debris (MMOD) particle. Among the vehicles of importance to NASA is the extravehicular mobility unit (EMU) spacesuit used while performing a US extravehicular activity (EVA). An EMU impact is of great concern as a large leak could prevent an astronaut from safely reaching the airlock in time resulting in a loss of life. For this reason, a risk assessment is provided to the EVA office at the Johnson Space Center (JSC) prior to certification of readiness for each US EVA.

Description: No abstract available

Description: Human spaceflight logistics requirements are strongly driven by the daily living needs of the astronauts, including their biological functions. Oxygen, water and food are absolute requirements to sustain life and must be supplied at adequate rates. However, these rates can vary from day to day and from person to person. Beyond the body's immediate physical needs, water is also required for important health and hygiene functions within the spacecraft. Undesirable weight loss or gain aside, human waste product mass outputs will equal the inputs over time, resulting in an average astronaut mass balance. Best values, as well as range of variability for inputs and outputs are explored at both the individual physiological level and the spacecraft level. These values are important for design of life support and habitability systems as well as for mission planning of consumables. Current spacecraft life support systems are not fully closed loop, but the International Space Station (ISS) does recycle most of its air and water. The astronaut mass balances at the personal and vehicle level can have different impacts at different levels of system closure. Recommendations are made for a consistent set of values representing a realistic average astronaut mass balance over reasonable durations for exploration missions.

Description: This document is the final report resulting from the work conducted by undergraduate students at the University of South Alabama during the 2018/2019 academic year and was prepared by the undergraduate students. As NASA pushes the boundaries further into space, the current technologies within the various life support systems must be improved upon. One such improvement is needed to the current air revitalization systems, specifically sorbents that can capture CO2 more effectively from enclosed habitats. Ionic liquids (ILs) have been considered as absorbents for flue gas, but little research has been done to test the ability of ILs at ambient pressures and relatively low concentration of CO2. The experiment outlined below utilizes the task-specific ionic liquid, tetramethylammonium taurinate (TMN), in a commercial off the shelf absorption system to capture CO2. The CO2 stream is combined with nitrogen to produce an inlet gas concentration relevant to close air revitalization applications. At an inlet gas flow with a CO2 partial pressure of 3.8 torr the system was capable of removing just under 97% of the inlet CO2. The concentration of CO2 in the outlet stream, partial pressure 0.16 torr, was less than that of atmospheric air. The duty required to separate the absorbed gas from the ionic liquid as well to cool the ionic liquid to be reintroduced to the column were acquired utilizing laboratory cooling/heating baths. These results show that TMN may be an efficient candidate for consideration in closed air revitalization.

Description: Every day in aviation, pilots, air traffic controllers, and other front-line personnel perform countless correct judgments and actions in a variety of operational environments. These judgments and actions are often the difference between an accident and a non-event. Ironically, data on these behaviors are rarely collected or analyzed. Data-driven decisions about safety management and design of safety-critical systems are limited by the available data, which influence how decision makers characterize problems and identify solutions. Large volumes of data are collected on the failures and errors that result in infrequent incidents and accidents, but in the absence of data on behaviors that result in routine successful outcomes, safety management and system design decisions are based on a small sample of nonrepresentative safety data. This assessment aimed to find and document safety successes made possible by human operators. With many Aeronautics Research Mission Directorate (ARMD) Programs and Projects focusing on increased automation and autonomy and decreased human involvement, failure to fully consider the human contributions to successful system performance in civil aviation represents a significant risk a risk that has not been recognized to date. Without understanding how humans contribute to safety, any estimate of predicted safety of autonomous capabilities is incomplete and inherently suspect. Furthermore, understanding the ways in which humans contribute to safety can promote strategic interactions among safety technologies, functions, procedures and the people using them. Without this understanding, the full benefits of an integrated, optimized human/technology or autonomous system will not be realized. Historically, safety has been consistently defined in terms of the occurrence of accidents or recognized risks (i.e., in terms of things that go wrong). These adverse outcomes are explained by identifying their causes, and safety is restored by eliminating or mitigating these causes. An alternative to this approach is to focus on what goes right and identify how to replicate that process. Focusing on the rare cases of failures attributed to human error provides little information about why human performance routinely prevents adverse events. Hollnagel has proposed that things go right because people continuously adjust their work to match their operating conditions. These adjustments become increasingly important as systems continue to grow in complexity. Thus, the definition of safety should reflect not only avoiding things that go wrong but ensuring that things go right. The basis for safety management requires developing an understanding of everyday activities. However, few mechanisms to monitor everyday work exist in the aviation domain, which limits opportunities to learn how designs function in reality. This concept of safety thinking and safety management is reflected in the emerging field of resilience engineering. According to Hollnagel, a system is resilient if it can sustain required operations under expected and unexpected conditions by adjusting its functioning prior to, during, or following changes, disturbances, and opportunities. To explore positive behaviors that contribute to resilient performance in commercial aviation, the assessment team examined a range of existing sources of data about pilot and air traffic control (ATC) tower controller performance, including subjective interviews with domain experts and objective aircraft flight data records. These data were used to identify strategies that support resilient performance, methods for exploring and refining those strategies in existing data, and proposed methods for capturing and analyzing new data.

Description: NASA's Project Mercury began as a response to the cold war with the Soviet Union and had a number of goals: to place a manned spacecraft in orbital flight around the earth; to investigate man's performance capabilities and his ability to function in the environment of space and to recover the man and the spacecraft safely. One aspect of preflight testing included the use of an altitude chamber to test each capsule and allow the astronauts to engage in simulated missions within a vacuum environment. Flash forward to 1985. The Biomedical Operations and Research Office at Kennedy Space Center proposed to use the chamber for an unusual mission under what was known as the Controlled Ecological Life Support Systems (CELSS)Breadboard Project. During 1985 into 1987, the chamber was converted to an environmentally-controlled, hydroponic plant growth chamber termed the "Biomass Production Chamber" and operated through late 2001.

Description: This paper discusses the current focus of NASA's Advanced Space Suit Pressure Garment Technology Development team's efforts, the status of that work, and a summary of longer term technology development priorities and activities. The Exploration Extra-vehicular Activity Unit (xEMU) project's International Space Station Demonstration Suit (xEMU Demo) project continues to be the team's primary customer and effort. In 2018 the team was engaged in addressing hardware design changes identified in the Z-2 pressure garment prototype Neutral Buoyancy Laboratory (NBL) test results. These changes will be discussed. Additionally components whose first iterations were produced in 2018 will be discussed. A full pressure garment prototype, termed Z-2.5, was assembled that is composed of updated and first prototype iteration hardware. Z-2.5 NBL testing, performed from October 2018 through April 2019 will inform final design iterations in preparation for the xEMU Demo preliminary design review planned to occur in the third quarter of government fiscal year 2019. A primary objective of the Z-2.5 NBL testing is to validate changes made to the hard upper torso geometry, which depart from the planetary walking suit upper torso geometry that has been used over the last 30 years. The team continues to work technology development, with GFY2018 work being used to supplement and feed the gaps left by the scope defined for the xEMU Demo. Specifically, a Phase IIx Small Business Innovative Research Grant to mature durable bearings that are compatible with a dust environment and a grant funded by the Science Technology Mission Directorate, Lightweight and Robust Exploration Space Suit (LARESS) project, to mature planetary impact requirements and hardware will be described. Finally, a brief review of longer-term pressure garment challenges and technology gaps will be presented to provide an understanding of the advanced pressure garment team's technology investment priorities and needs.

Description: Cyanobacterial and Harmful Algal Blooms (CyanoHABs) are a growing concern in coastal and inland waters. But, spectral interference from multiple constituents in optically complex waters can hamper application of remote sensing using traditional image processing methods. The Kent State University (KSU) spectral decomposition method can be applied to multispectral and hyperspectral remote sensing images (e.g. HICO and the NASA Glenn HSI2) to partition and identify signals related to cyanobacteria, algae, pigment degradation products and suspended sediment in each pixel. Fundamental to the use of remote sensing data is the ability to extract independent signals from correlated hyperspectral VNIR data cubes. The Kent State University varimax-rotated, principal component analysis method (VPCA) is important to integrate into the SBG VNIR mission concept because it provides greater specificity, a software-based SNR boost relative to hardware performance, and can assist with Cal/Val, Modeling and Applications. We present examples of the hyperspectral application of the KSU VPCA method with relevance to SBG. The information extracted by VPCA can be validated spectrally or spatially with laboratory and/or in situ sensors, which capture spatial or time series of information at discrete points within remote sensing images. Comparisons show hyperspectral sensors extract more components than multispectral ones, but more independent information can be extracted from multispectral sensors by VPCA than traditional band ratio approaches. The spectral decomposition method is capable of enhancing the signal to noise ratio (SNR) of the NASA Glenn, second-generation hyperspectral imager by a factor of 7x to 20x, with a spectral reproducibility of 3%. The spectral decomposition method, when compared against existing remote sensing monitoring methods exhibits both greater specificity and a lower detection limit. The method has been validated with multispectral images in Lake Erie to quantify the Microcystis CyanoHAB and from the Indian River Lagoon, Florida to quantify the Brown Tide resulting from A. lagunesnsis. Field operations in the Western Basin of Lake Erie were conducted using a bbe Fluoroprobe to collect vertical profiles and horizontal tows along a transect from the Toledo to the Detroit Lighthouse during coincident satellite overpasses. Extraction of pixel values from the MODIS Aqua sensor yields agreement between in situ field and lab-based measures of cyanobacterial, cryptophyte, diatoms and green algae, suspended sediment and pigment degradation products with R2〉0.8.

Description: Inertial acceleration and a change in head orientation with respect to gravity are sensed by mechanosensitive receptors in the inner ear otolith organs. These structures consist of calcium carbonate grains called otoconia that mechanically load the hair cell bundles and distribute the tangential shear force during movement, and changes in their density can alter hair cell sensitivity. A possible adaptive response to a chronic gravity change is a change in weight-lending otoconia. Another mechanism is a modification of the strength and number of synapses coupling the hair cells to nerve afferents that convey the signals into the brain. Here, we present the results obtained in 2 species exposed both to G (microgravity) and hyper-gravity (HG). Adult toadfish, Opsanus tau, were exposed to G (microgravity) in 2 shuttle missions and to 1.12-2.24G (force of gravity) [resultant] centrifugation for 1-32 days; readaptation was studied following 1-8 days after return to 1G. Results show a biphasic pattern in response to 2.24G: initial hypersensitivity, similar to that observed after G (microgravity) exposure, followed by transition to a significant decrease at 16-32 days. Recovery from HG exposure is approximately 4-8 days. Two major pieces of information are still needed: vertebrate hair cell response to altered gravity and impact of longer duration exposures on sensory plasticity. To address the latter we applied electron microscopic techniques to image otoconia mass obtained from 1) mice subjected to 91-days of G (microgravity) in the Mouse Drawer System (MDS) flown on International Space Station, 2) mice subjected to 91-days of 1.24G centrifugation on ground, and 3) mice flown on 2 shuttle missions. Images from MDS mice indicate a clear restructuring of individual otoconia, suggesting deposition to the outer shell. Images from their HG ground counterparts indicate the converse - an ablation of the otoconia mass. For 13-day exposures to G (microgravity) mice otoconia appear normal. Despite the permanence of gravity in evolution the animal senses exposure to a novel, non-1G, environment and adaptive mechanisms are initiated - in the short term compensation is likely confined to the peripheral sensory receptors, the brain or both. For longer exposures structural modifications of the otolith mass may also result.

Description: No abstract available

Description: The processed and prepackaged spaceflight food system is a critical human support system for manned space flights. As missions extend longer and farther from Earth over the next 20 years, strategies to stabilize the nutritional and sensory quality of food must be identified. For a mission to Mars, the space foods themselves must maintain quality for up to 5 years to align with cargo prepositioning scenarios. Optimizing the food system to achieve a 5year shelf life mitigates the risk of an inadequate food system during extended missions. Because previous attempts to determine a singular pathway to a 5year shelf life for food were unsuccessful, this investigation combines several approaches, based on science, technological advancement, and past empirical evidence, to determine their potential to extend the shelf life of the prepackaged food system for long duration missions. This study may identify food processing, packaging, and storage technologies that will be required for exploration missions and the extent that they must be implemented to achieve a 5year shelf life for the entire food system.

Description: This is a short presentation as part of a discussion panel on feeding Mars at the Humans to Mars summit. All slides are from previous presentations but they have been updated and organized into the shorter format.

Description: NASA's GeneLab includes an open-access repository of some 200 plus omics datasets generated by biological experiments relevant to spaceflight (including simulated cosmic radiation and microgravity). In order to maximize the intelligibility of these data, particularly for users with limited bioinformatics knowledge, GeneLab is now transforming the data in the repository into actual biological and physiological knowledge of the genetic and proteomic signatures found in these samples. This processed data is being derived by establishing standard data analysis workflows vetted by 114 scientists who are members of the four GeneLab Analysis Working Groups (Animal AWG, Plant AWG, Microbe AWG, Multi-Omics AWG). AWG members from institutes spanning the U.S. and four other countries participate on a voluntary basis. The AWGs meet monthly to discuss data mining, compare results and interpretations, and test forthcoming releases of the GeneLab Data Systems (GLDS). GLDS version 3.0 has been available to the general public since October 1st 2018, and has been providing a professional state-of-the-art bioinformatics platform for everyone in the space biology community to upload their data into a space biology omics data commons, to process their data with vetted standard workflows and to compare to existing analyses. The user interface for the platform is being designed to be accessible to a broad variety of users including those with limited bioinformatics experience, including high school and college students who can use it to learn about omics data analysis and space biology. As such, Genelab will constitute a powerful general public outreach capability of NASA and the Space Biology community at large. Data mining of the GeneLab database by the AWG has already started generating very interesting findings, including reports linking specific spaceflight conditions such as radiation, microgravity or carbon dioxide levels to molecular changes seen across various species. In this presentation, we will report on the current and future objectives for GeneLab, and review recent studies reported by the various AWGs relating molecular changes observed in various animal models and tissue with microgravity, radiation, circadian rhythm, hydration and carbon dioxide conditions.

Description: Pleated panel filters offer a new commercial form factor for controlling VOCs in spacecraft cabin air. They differ from conventional commercial granular activated carbons because they have a lower pressure drop across the filter. A testbed was developed for evaluating the removal capacities of commercial pleated panel filters for NH3. The adsorptive capacity of a commercial cation-exchange pleated filter was compared versus the adsorptive capacities of two acid- impregnated activated carbons used for controlling ammonia in spacecraft cabin air.

Description: Crops for space life support systems and in particular, early supplemental food production systems must be able to fit into the confined volume of space craft or space habitats. For example, spaceflight plant chambers such as Svet, Lada, Astroculture, BPS, and Veggie provided approximately 15-40 cm of growing height for plant shoots. Six cultivars each of tomato and pepper were selected for initial study based on their advertised dwarf growth and high yields. Plants were grown in 10-cm pots with solid potting medium and controlled-release fertilizer to simulate the rooting constraints that might be faced in space environments. Lighting was provided by fluorescent lamps (~300 umol m(exp -1) s(exp -1) and a 16 h light / 8 h dark photoperiod. Cultivars were then down selected to three each for pepper (cvs. Red Skin, Pompeii, and Fruit Basket) and tomato (cvs. Red Robin, Mohamed, and Sweet n' Neat). In all cases (pepper and tomato), the plants grew to an approximate height of 20 cm and produced between 200 and 300 g fruit fresh mass per plant. In previous hydroponic studies with unrestricted root growth, Fruit Basket pepper and Red Robin tomato produced much larger plants with taller shoots. The findings suggest that high value, nutritious crops like tomato and pepper could be grown within small volumes of space habitats, but horticultural issues, such as rooting volume could be important in controlling plant size.

Description: Gravity is an omnipresent force on Earth, and all living organisms have evolved under the influence of constant gravity. Mechanical forces generated by gravity are potent modulators of stem cell based tissue regenerative mechanisms, inducing cell fate decisions and tissue specific commitment. A novel mechanical unloading investigation assessed the formation, morphology, and gene expression of embryoid bodies (EB), a transitory cell model of early differentiation. After 15 days of spaceflight, the mechanotransduction-null EB cells showed upregulated proliferative mechanisms while differentiation cues were silenced.

Description: Silver has been selected as the forward disinfectant candidate for potable water systems in future space exploration missions. To develop a reliable antibacterial system that requires minimal maintenance, it is necessary to address relevant challenges to preclude problems for future missions. One such challenge is silver depletion in potable water systems. When in contact with various materials, silver ions can be easily reduced to silver metal or form insoluble compounds. The same chemical properties that make ionic silver a powerful antimicrobial agent also result in its quick inactivation or depletion in various environments. Different metal surface treatments, such as thermal oxidation and electropolishing, have been investigated for their effectiveness in reducing silver disinfectant depletion in potable water. However, their effects on the metal surface microstructure and chemical resistance have not often been included in the studies. This paper reports the effects of surface treatments on stainless steel 316 (SS316) exposed to potable water containing silver ion as a disinfectant. Early experimental results showed that thermal oxidation, when compared with electropolishing, resulted in a thicker oxide layer but compromised the corrosion resistance of SS316.

Description: Since the 1950s, mechanical counter-pressure (MCP) has been investigated as a possible alternative architecture to traditional extra-vehicular activity (EVA) suits. While traditional gas-pressurized EVA suits provide physiological protection against the ambient vacuum environment by means of pressurized oxygen to at least 3.1 psid, MCP provides protection by direct application of pressure on the skin by a fabric. In reviewing the concept, MCP offers distinct potential advantages to traditional EVA suits: lower mass, reduced consumables, increased mobility, increased comfort, less complexity, and improved failure modes. In addition, as basic feasibility was established in the 1960s with the successful testing of the Space Activity Suit, MCP seems poised to inevitably supplant traditional EVA architectures with a modest degree of concentrated development. However, as they say, "The devil is in the details". This paper serves as a comprehensive summary of the technical work that has been completed related to MCP from 1960 to 2019, the technical gaps that need to be closed to facilitate a flight-capable design, and outlines an overall development strategy that NASA feels would best address these gaps moving forward.

Description: No abstract available

Description: Cost-effective high reliability can be achieved in future space life support systems through careful systems analysis and design. This paper outlines a comprehensive approach. Potential future human space missions are described. The mission parameter impacts on life support system design and reliability requirements are discussed. Not all human space missions require high reliability life support. The potential reliability and cost of storage and of recycling life support systems are investigated. Simple storage systems can provide cost-effective high reliability life support where it is needed. More complex recycling systems with lower reliability and higher cost can be used when suitable.

Description: This presentation provides a status of the xEMU ISS Demo project and the approach to requirements definition related to certification and extensibility considerations.

Description: This presentation supports a Collaborative Discussion regarding industry's utilization of other NASA or external design standards and feedback and recommendations to support the possibility of an EVA suit standard.

Description: Mars is the crucial goal of human exploration beyond the Earth-moon system. The Mars round trip transit vehicle has been expected to use a regenerative Life Support System (LSS) similar to the one on the International Space Station (ISS). It often assumed that the Mars transit LSS will be operated on the outward trip to Mars, placed in dormancy while the full crew explores the surface, and then restored to operation for the return trip to Earth. The major difference between Mars missions and operations in the Earth-moon system is the need for much higher reliability for Mars missions, since rapid resupply of parts and materials or a quick crew return to Earth are not possible. Mars systems must achieve intrinsic high reliability by design, test, failure analysis, and redesign and then increase operational robustness by providing spare parts and redundant systems. Further requiring the LSS to be capable of dormancy and restoration to operation greatly increases the difficulty of design, test, and verification. The process of implementing dormancy and then restoring operation would add significant risk to the mission. Dormancy should be avoided for Mars and can be avoided several ways. First and most obvious, some crew can remain continually on board. If no crew can remain onboard, dormancy can still be avoided if an unused spare LSS is activated for the return trip, rather than restarting the used out bound system. Systems similar to the ISS LSS would have a significant probability of failure on a Mars trip and therefore would require two or three spares. Another full spare LSS could be provided as the return trip system, rather than refurbishing a used LSS.

Description: Biomechanical data collection and modeling has applications to the field of human factors. Specifically, motion data can be used to determine the operational volume necessary for performing a task. The operational volume assessment can be performed in order to determine how much volume is needed to perform the task or if task performance can be contained and adequately performed within an allocated volume. Motion and external force data, along with computational modeling techniques, can be used to estimate the internal loading produced during performance of a task. Internal loading estimates can be used to determine if an adequate stimulus is generated for maintenance of musculoskeletal health and also for comparison to injury thresholds to determine injury risk during task performance.

Description: Several dwarf tomato and pepper varieties were evaluated under ISS-simulated growth conditions (22C, 50% RH, 1500 ppm CO2, and 300 mol m(exp -2) s(exp -1) of light for 16 h per day) with the goal of selecting those with the best growth, nutrition, and organoleptic potential for use in a pick and eat salad crop system on ISS and future exploration flights. Testing included six cultivars of tomato (Red Robin, Scarlet Sweet N Neat, Tiny Tim, Mohamed, Patio Princess, and Tumbler) and six cultivars of pepper (Red Skin, Fruit Basket, Cajun Belle, Chablis, Sweet Pickle, and Pompeii). Plants were grown to an age sufficient to produce fruit (70 to 106 days for tomato and 109 days for pepper). Tomato fruits were harvested when they showed full red color, beginning ca. 70-days age and then at weekly intervals thereafter, while peppers were grown until numerous fruits showed color and all fruits (green and colored) were harvested once at the end of the test. Plant sizes, yields, and nutritional attributes were measured and used to down-select to three cultivars for each species. In particular, we were interested in cultivars that were short (dwarf) but still produced high yields. Nutritional data included elemental (Ca, Mg, Fe, and K) composition, vitamin K, phenolics, lycopene, anthocyanin, lutein, and zeaxanthin. The three down-selected cultivars for each species were evaluated for sensory attributes, including overall acceptability, appearance, color intensity aroma, flavor and texture. The combined data were compared and given weighting factors to rank the cultivars as potential candidates for testing in space. For tomato, the ranking was 1) cv. Mohamed, 2) cv. Red Robin, and 3) cv. Sweet N Neat. For pepper, the ranking was 1) cv. Pompeii, 2) cv. Red Skin, and 3) cv. Fruit Basket. These rankings are somewhat subjective but provide a good starting point for conducting higher fidelity testing with these crops (e.g., testing with LED lighting similar to the Veggie plant unit), and ultimately conducting flight experiments.

Description: Recycling waste has been an issue on Earth for decades. The OSCAR project seeks to find ways to make sure that it does not become an issue in space. The main focus of OSCAR is the combustion of waste and reclamation of gaseous products in microgravity. The first phase of testing relies on a ground rig that operates both under normal (Earth) gravity and in drop tower tests that briefly simulate a microgravity environment. In the second phase, a test will be performed during a suborbital flight were the experiment will be carried out in microgravity. Throughout the spring term, interns have played an integral part in continuing the progress made by the project. They performed work in upgrading the electrical and mechanical systems that make up OSCAR. They made multiple improvements to the test rig's operating software to improve readability and usability. They prepared and edited documents that were vital to the engineering process. And, they were responsible for performing lab tests and refining the lab operations document and procedure. The interns were a big help in maintaining the rigorous test schedule. OSCAR, which stands for Orbital Syngas Commodity Augmentation Reactor, is to find a way to turn astronaut waste into chemical energy. The two parts of this are important: finding a way to dispose of waste generated in space, and seeing if there is a way to recycle that waste into chemical energy. The importance of the disposal aspect is that there is currently no way to dispose of, or recycle, waste that is created in space other than jettisoning it (which is what the ISS does via empty supply capsules). As manned missions go deeper into space, that method will no longer be viable, as a craft would essentially be littering the space and planets that they visit. Energy reclamation is also important because of the high monetary and spatial costs of sending supplies on space missions. Every little bit extra that can be reused out of what is sent can save room and funds for other supplies. The facet of this problem that the OSCAR project is focusing on is how to combust waste in zero gravity. Combustion in the presence of gravity is one of those things that is taken for granted. When something burns on Earth, the flames rise above the fuel as oxygen flows from underneath. In microgravity, the flames surround the object completely, which restricts the amount of oxygen that can reach the fuel, and retards the combustion. OSCAR uses a vortex reaction chamber to counter this phenomenon. The OSCAR test rig will eventually be tested on a suborbital flight to see if it is an effective solution to the issue in real-world conditions. Currently, there is a prototype test rig that is fully functional. This rig has been previously tested in a 2 second drop test at Glenn Research Centers (GRC) Zero Gravity Facility (ZGF). (The free-fall conditions of the drop mimic microgravity, if only for a brief period of time). This sessions focus was on upgrading the test rig and software, updating the paperwork, performing additional lab tests, and readying the rig for the five second drop test, again at GRC. II. Upgrades The state of the testing rig at the start of the session was in between its configurations for the two second drop tower and the five second drop tower. The rig needed upgrades to address various insufficiencies that either were discovered during the two second campaign or were a direct result of the differences between the two drop tower setups. The main differences that had to be handled were the increase in shock loads from 30g to 65g, a difference in drop indicating signal (on the falling edge of a pulse instead of a change from high to low), and the ambient pressure of the test apparatus (the two second tower dropped the rig in atmosphere, while the five second tower drops in vacuum).

Description: Several cultivars of dwarf tomatoes and dwarf peppers were studied as possible candidate for space crops. Results showed the tomato cvs. Red Robin, Mohamed, and Sweet 'N' Neat produced the greatest yields, while pepper cvs. Pompeii, Red Skin, and Fruit Basket produced the greatest yields. The tomato and pepper cultivars were also analyzed by taste panels for organoleptic attributes, and all the cultivars were found to be acceptable by the taste panelists.

Description: Since the 1950s, mechanical counter-pressure (MCP) has been investigated as a possible alternative design concept to traditional extra-vehicular activity (EVA) space suits. While traditional gas-pressurized EVA suits provide physiological protection against the ambient vacuum by means of pressurized oxygen to at least 3.1 pounds per square inch absolute (160 millimeters of mercury), MCP provides protection by direct application of pressure on the skin by a fabric. In reviewing the concept, MCP offers distinct potential advantages to traditional EVA suits: lower mass, reduced consumables, increased mobility, increased comfort, less complexity, and improved failure modes. In the mid 1960s to early 1970s, Dr. Paul Webb of Webb Associates developed and tested such a suit under funding from NASA Langley Research Center. This "Space Activity Suit" (SAS) was improved many times while testing in the laboratory and an altitude chamber to as low as 0.3 pounds per square inch absolute (15 millimeters of mercury). This testing, and the reports by Webb documenting it, are often presented as evidence of the feasibility of MCP. In addition, the SAS reports contain a wealth of information regarding the physiological requirements to make MCP work at the time, which is still accurate today. This paper serves to document the Space Activity Suit effort and analyze it in today's context.

Description: In 2017, our team investigated and evaluated the novel concept of operations of astronaut self-scheduling (rescheduling their own timeline without creating violations) onboard International Space Station (ISS). Five test sessions were completed for this technology demonstration called Crew Autonomous Scheduling Test (CAST). For the first time in a spaceflight operational environment, an ISS crewmember planned, rescheduled, and executed their activities in real-time on a mobile device while abiding by flight and scheduling constraints. This paper discusses the lessons learned from deployment to execution.

Description: Historically, competitions and prizes such as those executed by the NASA Centennial Challenges (CC) program have created broader avenues through which to spur innovation from unlikely sources. In 2005, Congress amended the National Aeronautics and Space Act of 1958 to authorize NASA to create challenges through which prizes could be awarded to United States citizens or entities that succeeded in meeting the challenge objectives. Over the past 13 years, the CC program has initiated more than 19 challenges in a variety of technology areas, including propulsion, robotics, communications and navigation, human health, science instrumentation, nanotech, materials/structures and aerodynamics. This paper will discuss the status and the accomplishments of the CC program and discuss results of an ideation process designed to identify and formulate topics for a potential Centennial Challenge competition targeting a life support technology gap for future long-term exploration missions. Status of this challenge formulation process with information on how to use crowdsourcing tools will be discussed. An overview of the CC Programs accomplishments, including strategic objectives, past challenges, and current challenge development and execution. This program exemplifies the values that have formed the bedrock of the culture at NASA since the beginning: innovation, imagination, and a passion for exploration.

Description: No abstract available

Description: On the International Space Station (ISS) there are currently two toilets. One is located in the Russian Service Module and the other is located in the U.S. segment's Node 3. A new Exploration Toilet will be integrated next to the existing Node 3 Waste and Hygiene Compartment (WHC). The Toilet will be evaluated as a technology demonstration for a minimum of three years. In addition, it will support an increase in ISS crew size due to Commercial Crew flights to ISS. The Toilet is designed to minimize mass and volume for Orion, the first Exploration vehicle. Currently ISS does not have a designated volume for an additional Toilet. Furthermore, operating the Toilet on ISS presents a different set of challenges as it must integrate into existing vehicle systems for urine processing. To integrate the Toilet on ISS, a suite of hardware was developed to provide mechanical, electrical, data, and fluid interfaces. This paper will provide an overview of the Toilet Integration Hardware design as well as the engineering challenges, crew interface provisions and vehicle integration complexities encountered during the concept and design phases.

Description: Supplemental safe food production has been an essential goal of NASA to meet the nutritional needs of astronauts on the International Space Station (ISS) as well as for future long duration missions to the moon and beyond. Food crops grown in space experience different environmental conditions than plants grown on Earth (i.e. microgravity and spaceflight physical sciences impacts). To test the growth methods and effects of the space environment, red romaine lettuce Lactuca sativa cv. 'Outredgeous', was grown in Veggie plant growth chambers on the ISS. Microbiological food safety of the plants grown on the ISS was determined by heterotrophic plate counts to assess total microbial load for bacteria and fungi as well as screening for specific pathogens and isolate identification. Molecular characterization was completed using Next Generation Sequencing (NGS) to provide valuable information on the taxonomic composition and community structure of the plant microbiome. Chemical analyses of plant tissue were conducted to understand spaceflight-induced changes in key elements in the space diet, phenolics, anthocyanin levels, and Oxygen radical absorbance capacity (ORAC), a measure of antioxidant capacity. Three growth tests of red romaine lettuce were completed on ISS, VEG-01A, VEG-01B, and VEG-03A. Plants were harvested using two harvest methods, either a single terminal harvest (after 33 days) or cut-and-come-again repetitive harvesting (64 days total growth). Ground controls were grown simultaneously with a delay to accommodate condition monitoring and replication. A comparison of the plant tissue returned to Earth showed leaves from the second grow-out had significantly higher bacterial counts than the preceding or subsequent growth test or any of the ground controls. Fungal counts were significantly higher on the final cut-and-come-again harvest of the third grow out. None of the potential foodborne pathogens that were screened for were detected. Bacterial and fungal isolate identification and community characterization indicated similar diversity between VEG-01A and VEG-01B growth tests, however, there appeared to be subtle differences in diversity and distribution among the three growth tests. Chemical analysis of plant tissue revealed significant variation in a few elemental data, but variation in levels of phenolics, anthocyanins, and ORAC was not significantly different. This study indicated that leafy vegetable crops could safely provide an edible supplement to astronauts' diet, and our analysis provided baseline data for continual operation of the Veggie plant growth units on ISS. This research was funded by NASA's space biology program.

Description: As the agency focuses on lunar missions, it is important to revisit the human factors and behavioral performance (HFBP) challenges for long duration exploration missions. We outline the important factors from the Apollo program, the long duration experience gained onboard International Space Station (ISS), and HFBP research applicable to exploration-class missions.

Description: The vestibulospinal system provides the spinal motor circuits controlling head/neck and limb movements and body posture with rapid reflex adjustments to maintain equilibrium and stability and with a continuous essential excitatory drive, called tonus, to enhance reactive responses to perturbations that force the animal off normal posture. The sensory signals to these reflex circuits originate from hair cells in the inner ear of otolith structures, namely the utricle and saccule, that transduce inertial acceleration and orientation of the head with respect to gravity and in the three orthogonally arranged semicircular canals that transduce angular head rotation. The principal vestibulospinal pathways are 1) the medial vestibulospinal tract that descends in the ventromedial funiculus and innervates inter- and motoneurons located mainly in lamina VII, VIII, and dorsomedial IX throughout the cervical segments; and 2) the lateral vestibulospinal tracts that course in the lateral to ventrolateral funiculi and are distinguished by two divisions: i) a cervical-projecting tract that overlaps many of the targets of medial vestibulospinal tract neurons including the motoneurons in ventromedial IX and also contributes to reflex control of shoulder and forelimb (arm) muscles; and ii) a lumbosacral-projecting tract that provides a rapid input to maintain stable posture and reflex control of the lower body. A striking observation in understanding the functional organization of this sensory-motor system is both that the driving sensory input can be dynamically modified by the behavioral context in which the sensation is made and that it remains able to quickly respond to an external force during self-generated head movements. The structural basis for vestibulospinal inputs to spinal motor control circuits in quadrupeds and bipeds rely in part on the animal's need for coordination between fore- and hind-limb reflex movements. Understanding the sensory-to-motor transformations in the diverse species rely on the correlations of the conserved and unique species behavior, morphology and physiologic function.

Description: The Extravehicular Activity (EVA) Framework for Exploration describes NASA's EVASystem Goals in the broader context of ongoing human spaceflight efforts. The purpose of thisdocument is to drive integration, coordination and communication of the EVA community'sexploration development plans as crafted to meet long-term EVA needs. Inclusive in the EVAcommunity are NASA partners in academia and industry. The 2019 EVA Framework outlinesthe office's current method to answer the following questions: What product does NASA useto compare, contrast and integrate across the elements of the EVA community's perceivedgaps, risks, and unfunded work, particularly for future systems intended for use beyond LowEarth Orbit (LEO)? What product does NASA use to proactively coordinate support acrossthe EVA community's wide spectrum of exploration development work? Where can one go toobtain awareness of ongoing efforts, particularly during consideration of new-start activitiesand proposals? These questions lead to the need for a product that speaks to the distributednature of the EVA System across human spaceflight programs, concept studies and flightvehicle architectural elements. This framework can be used and evaluated by the EVAcommunity to assess the full spectrum of needs and answer the question of "what are wemissing" or "are we doing things that just do not make sense". In the end is the EVAcommunity effectively pursuing the future needs of EVA? If answers to those questions revealthe need for change or re-prioritization then actions can be taken through existing projectcontrol processes as well as revision to this document and supporting project plans.

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Description: A review of NASA's bioregenerative life support research will be presented along with testing related to Mars greenhouse or plant growth systems.

Description: The ability to predict cancer risk associated with exposure to low doses of high-LET ionizing radiation (IR) remains a challenge. Epidemiological methods lack the sensitivity and power to provide detailed risk estimates for cancer and ignore individual variance in IR sensitivity. We have hypothesized that DNA repair capacity can be used as a marker to evaluate and differentiate individual radiation sensitivity. More specifically, this work is based on the concept that the combined time-dose dependence of radiation-induced foci (RIF) of p53-binding protein 1 (53BP1) following low-LET exposure contains sufficient information to infer sensitivity to any other LET. Our hypothesis was tested in 15 different mouse strains as well as in primary human immune cells. We first approached individual ionizing radiation sensitivity in a mouse model by culturing primary skin fibroblasts extracted from 76 mice of 15 different genetic backgrounds and exposing them to HZE particles and X-rays. This work is one of the most extensive studies on the kinetics and possible genetic underpinnings of radiation-induced DNA damage and repair. Our results is in agreement with a DNA repair model we previously postulated, where nearby DNA double strand breaks (DSB) in the nucleus are brought together for more efficient repair, leading to RIF clustering. Such mechanism was evidenced by a specific dose and LET dependence of RIF numbers. Briefly, RIF quantification after low-LET X-ray exposure showed an asymptotic saturation for doses between 1 Gy and 4 Gy 4 hours post-irradiation across all 15 strains. The clustering of DSB across all strains also led to more RIF/Gy for lower LET (X-ray and 350 MeV/n Ar) than for higher LET (600 MeV/n Fe) 4 hours post-exposure. Considering the fact that the number of DSB/Gy should be independent of LET, our data suggest there are more DSB in individual RIF as the LET increases. RIF numbers for 24 and 48 hours post-exposure led to the inverse trend, with more remaining RIF/Gy for higher LET (by 600 MeV/n Fe). This result suggests cells have more difficulty resolving RIF from higher LET as they the number DSB/RIF increases. Note that for most conditions, the variance of RIF/Gy was small within individual animals of the same strain and large between strains, suggesting a strong genetics component. Furthermore, we present our preliminary data from an ongoing study on human genetic associations with IR sensitivity. To address the human variability in responses to HZE particle irradiation in a maximally comprehensive manner, we are in the process of collecting and isolating primary blood mononuclear cells from 768 healthy subjects of European descent, 18-75 years of age, 50/50 male/female distribution. We have analyzed 53BP1+ RIF formation as well as oxidative stress and cell death in primary cells from 192 subjects in response to the same HZE particles as used in mice: 600 MeV/n Fe, 350 MeV/n Ar and 350 MeV/n Si, 1.1 and 3 particles/100m2, 4 and 24 hours after irradiation. We will next complete the quantification of HZE particle-induced DNA and cellular damage in the remaining subjects and compare it to their responses to low-LET irradiation. Finally, we will perform GWAS analysis to identify human genomic associations with IR sensitivity and potential targets for biomarker development.

Description: The purpose of this NCRP commentary is to provide the current state of knowledge on the effects of ionizing radiation on the immune system and on latent herpes virus reactivation to the scientific community and government agencies. Its purpose is to better understand radiation-induced latent virus reactivation, which is possibly an underestimated consequence of ionizing radiation exposure. This activity should involve the radiation research community (academia, industry and regulatory agencies) and government agencies (NASA, DOD, CDC).

Description: During the late summer, the author sailed to the Antarctic South Shetland Islands to survey the microorganisms living in marine (tidal pools) and freshwater (moss saturated with snow melt) environmental niches. Equipped with a microscope to take video of samples within hours of collection to capture a pristine condition, the authors found a dense and diverse ecology that included species with unique patterns of locomotion. Capturing the organism's movement expedited identification, but it also showed the dynamic way each organism's mobility fit together like a puzzle to create a complex ecosystem.

Description: This presentation will be an introduction and overview of space crop production needs, goals, and challenges in the areas of robotics and automation for the workshop Aug. 6-7, 2019 at Kennedy Space Center. This presentation will be used to start the workshop and set the direction.

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Description: The BioBot concept consists of a robotic rover which is capable of traversing the same terrain as a spacesuited human. It carries the primary life support system for the astronaut, including consumables, atmosphere revitalization systems (e.g., CO2 scrubbing, humidity and temperature management, ventilation fan), power system (e.g., battery, power management and distribution),and thermal control system (e.g., water sublimator, cooling water pump), along with umbilical lines to connect to the supported astronaut. Although not technically part of life support, it would be logical for the BioBot to also provide long-range communications, video monitoring, tool and sample transport, and other functions to enable and enhance EVA productivity in planetary surface exploration.The design reference scenario for this concept is that astronauts involved in future lunar or Mars exploration will be on the surface for weeks or months rather than days, and will be involved in regular EVA operations. It is not unreasonable to think of geologists spending several days inEVA exploration each week over a prolonged mission duration, with far more ambitious operational objectives than were typical of Apollo. In this scenario, each astronaut will be accompanied by a "BioBot", which will transport their life support system and consumables, an extended umbilical and umbilical reel, and robotic systems capable of controlling the position and motion of the umbilical. The astronaut will be connected to the robot via the umbilical, carrying only a small emergency open-loop life support system similar to those contained in every PLSS. The robotic mobility base will be designed to be capable of traveling anywhere the astronaut can walk, and will also be useful as a transport for the EVA tools, science instrumentation, and collected samples. In addition, the BioBot can potentially carry the astronaut on traverses as well. Such a system will also be a significant enhancement to public engagement in these future exploration missions, as the robotic vehicles can also support high-resolution cameras and high bandwidth communications gear to providehigh-definition video coverage of each crew throughout each EVA sortie.

Description: NASA GeneLab is an open-access repository for omics datasets generated by biological experiments conducted in space or ground experiments relevant to spaceflight (e.g. simulated cosmic radiation, simulated microgravity, bed rest studies). The GeneLab Data Systems (GLDS) version 4.0 will be available on October 1st 2019, and will provide a state-of-the-art bioinformatics platform for the space biology and radiation communities to upload their data into an omics data commons, to process their data with vetted standard workflows and to compare with existing analyses. Started in 2015 as a repository designed to archive omics data from space experiments, GeneLab has expanded its scope to all ionizing radiation omics experiments conducted on the ground and has put considerable effort in providing carefully characterized radiation metadata on all datasets. GeneLab is also providing processed data derived from the raw data covering a large spectrum of omics (genome, epigenome, transcriptome, epitranscriptome, proteome, metabolome) to help users explore important questions: 1) Which genes or proteins are expressed differently in space for various living organisms? 2) What specific DNA mutations or epigenetic changes happen in space or after exposure to ionizing radiation? and 3) How does genetics affect these responses? Processed data available on GeneLab are derived by standard data analysis workflows vetted by hundreds of scientists who volunteered to join one of the four GeneLab Analysis Working Groups (Animal AWG, Plant AWG, Microbe AWG, Multi-Omics AWG). In this presentation, we will discuss how to bridge the gap between irradiation studies performed on earth and biological experiments conducted in space since the early 1990's. We will discuss how radiation dosimetry was estimated for datasets derived from samples collected during the Space Shuttle era on the International Space Station and on other orbiting platforms. Finally, we will address future strategies regarding dose monitoring in future missions into space, inter-agency efforts to unify data under one umbrella, and knowledge dissemination across the radiation research community and the space biology community.

Description: Future Exploration missions will require an Oxygen Generation Assembly (OGA) to electrolyze water to supply oxygen for crew metabolic consumption. The system design will be based on the International Space Station (ISS) OGA but with added improvements based on lessons learned during ISS operations and technological advances since the original OGA was designed and built. These improvements will reduce system weight, crew maintenance time and spares mass while increasing reliability. Currently, the design team is investigating the feasibility of the upgrades by performing ground tests and analyses. Upgrades being considered include: redesign of the electrolysis cell stack, deletion of the hydrogen dome, replacement of the hydrogen sensors, deletion of the wastewater interface, redesign of the recirculation loop deionizing bed and redesign of the cell stack Power Supply Module. The upgrades will be first demonstrated on the ISS OGA.

Description: The Advanced Concepts Office needed human factors analyses on various hatches for future deep space modules. The current standard is the 32" hatch, and the goal of this analysis was to assess this hatch size compared to larger sizes for egress, logistics, and safety.

Description: NASA Marshall Space Flight Center (MSFC) Human Factors Engineering (HFE) Team is implementing virtual reality (VR) and motion capture (MoCap) into HFE analyses of various projects through its Virtual Environments Lab (VEL). These techniques are being implemented for concept of development of Deep Space Habitats (DSH) and design and analyses for NASAs Space Launch System (SLS). VR utilization in the VEL will push the design to be better formulated before mockups are constructed, saving budget and time.

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Description: Methane and carbon monoxide are gaseous contaminants commonly found in a crewed spacecrafts cabin environment that are of interest to trace contaminant control equipment design. Generation sources include crew metabolism and equipment offgassing. Sources and generation rates of methane and carbon monoxide aboard the International Space Station (ISS) are examined. Cabin atmosphere concentration dynamics covering 19 years of ISS crewed operations are presented and correlation with octafluoropropane (Freon 218) concentration levels is analyzed.

Description: MSFCs Human Factors Engineering (HFE) team is responsible for all worksite analyses performed for the SLS pre-launch integration activities at Kennedy Space Center (KSC). There is a wide variety of tasks associated with pre-launch integration activities and it is important to verify that vehicle integration will be successful early in the design process. The VR work performed by the HFE team at MSFC has allowed fast changing layouts to be analyzed by various departments with minimal impact to cost or schedule. Implementing these methods for SLS allows for VR use in early design cycles, saving time and budget. Utilizing the resulting HFE analyses improves usability and safety. Ultimately, the goal is to provide a safe environment for the technicians assembling the vehicle and the astronaut crew at launch.

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Description: Molecular biosignatures are key targets for current, proposed, and future life detection missions. With the high accuracy and low limit of detection (LOD) that new and future instruments will require, decontamination of life detection hardware is necessary to prevent false positives. Lipids are a molecular biosignature of interest, as they are ubiquitous to all life as we know it, can survive unaltered in the geologic record for longer than any other biomolecule (i.e. billions of years), and form through both biotic and abiotic processes. Lipids display origin-diagnostic molecular patterns that can reveal biotic or abiotic synthesis, so finding them and ascertaining their molecular features is important for potentially detecting evidence of life elsewhere. Traditional methods of decontamination, or contamination control (CC), primarily clean hardware through fabrication in sterile (cleanroom) environments, killing microbes, and removing/flushing contaminants off instrument and spacecraft components. However, research suggests that some standard cleaning methods are either unlikely to remove lipid contaminants or are incompatible with life detection instrument materials. To solve this problem, I propose to find, test, and verify a decontamination method that thoroughly cleans instruments by destroying lipid molecules, but is simultaneously compatible with major materials used in these instruments. I will study the effects of traditional CC methods (including Dry Heat Microbial Reduction and Vapor phase Hydrogen Peroxide) and experimental CC methods (Electron Beam Irradiation) on lipid molecules for application to life detection instrumentation. I will then develop a CC plan for a novel lipid detector (ExCALiBR, Extractor for Chemical Analysis of Lipid Biomarkers in Regolith) searching for lipids in either soil or icy world scenarios. This plan will uphold planetary protection regulation requirements and validate experimental analyses of in-situ life detection tests.

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Description: Spacesuits allow humans to function in an incredibly harsh environment. However, they introduce some restrictions to human capabilities. In general, crewmembers in a spacesuit have a restricted maximal reach envelope, reduced field of view, and reduced tactility. When tasks and interfaces are being designed, they need to take into account the restrictions associated with working in an Extravehicular Activity (EVA) suit.

Description: The many known health risks currently associated with space travel include increased risk of cardiovascular disease, cancer, central nervous system related diseases, muscle degeneration, and changes with host-gut microbiome interactions that can have profound impact with these and other health risks. The majority of the risk from space travel stem of the two components of the space environment which are microgravity and radiation. Two specific systemic effects have been uncovered by us to impact the body as a whole due to the space environment. One factor is related from our earlier work (Beheshti et al, PLOS One, 2018), we predicted that there is a systemic component of the host that causes general increased health risks due to spaceflight driven by a circulating microRNA (miRNA) signature consisting of 13 miRNAs that directly regulates both p53 and TGF1. MiRNAs are small non-coding RNA molecules with a negative and post-transcriptional regulation on gene expression) are increasingly recognized as major systemic regulators of responses to stressors, including microgravity, oxidative stress, and DNA damage. In addition, due to the size and stability of miRNAs, it is known that miRNAs can circulate throughout the body and have been found in the majority of the bodily fluids including blood, urine, saliva, and tears. Here, we start to dissect the actual impact of this miRNA signature on both the radiation and microgravity components and prove that this miRNA signature actually exists in the circulation of a host. The other systemic factor we uncovered was the impact the mitochondria on the whole body due to spaceflight. We hypothesize that spaceflight may promote a physiologic response driven by systemic mitochondria pathways leading to metabolic disorder stemming from the liver and directly impacting other organs and tissues. A systems biology method was implemented utilizing GeneLab datasets that involved in vitro experiments performed at the low Earth orbit, in vivo experiments involving mice flown to space, and finally human physiological data from astronauts. A comprehensive multi-omics approach was implemented which involved correlating transcriptomic analysis with proteomics, metabolomics, and methylation analysis. This approach led us to confirm our hypothesis that a systemic mitochondrial driven response is responsible for increasing potential health risk and is conserved from the in vitro studies, to the in vivo studies, and finally confirmed in astronauts.

Description: Space crop production will be important in future long duration exploration missions to supplement the packaged diet with fresh bioactive nutrients. Plant care and the addition of fresh veggies to the diet may also have a role in astronaut well-being. Pick-and-eat salad crops are the best candidates for this near-term supplementation since they require minimal processing or preparation to add to meals. While light quality can strongly influence plant responses on Earth, the impacts of light quality on plant growth and composition in spaceflight remain unclear. The VEG-04 experiment uses two Veggie plant growth chambers on the International Space Station to simultaneously test different red: blue light ratios on the growth of Mizuna mustard, a leafy green salad crop. In addition to plant health and yield, the composition of key nutrients is assessed. Astronauts conduct on-board organoleptic evaluation of the fresh produce. Microbial food safety of returned produce is examined, and a Hazard Analysis Critical Control Point (HACCP) plan has been developed for this crop. VEG-04 consists of two experiments, one lasting 28 days with a single harvest, and the second lasting 56 days, with three cut-and-come-again harvests. These different scenarios provide an opportunity to test two production concepts, examine different fertilizers, monitor microbial changes over time for this crop, and assess potential impacts of interacting with plants on crew behavioral health and performance in spaceflight operations. In ground testing, plant growth was not significantly different across the different light treatments, however nutrient composition did differ significantly. Flight test results will be compared with ground data. This research was co-funded by NASA's Human Research Program and Space Biology in the ILSRA 2015 NRA call.

Description: The impact of spaceflight on immune function is undoubtedly a critical focus in the area of space biology and human health research. Heat shock proteins (Hsp) are an evolutionarily conserved family of proteins that are expressed in response to cellular and physiological stressors, experienced during radiation exposure, confinement, circadian rhythm disruption, and altered gravity (hypergravity experienced at launch/landing and microgravity experienced in-flight). In particular, Hsp70 aids in the folding of proteins, facilitates the movement of proteins across the membranes during signal transductions and can stimulate innate immunity. Since Hsp70 is induced during cellular stress, and can act as a stimulator for innate immunity, we sought to address how a loss of Hsp70 affects immunity, under the stress-inducing model of acute and chronic hypergravity. Moreover, the effects of gravity as a continuum on the induction of Hsps and key immune genes were also assessed to determine if increased cellular stress, via increased gravity (g)-force, contributes to immune dysfunctions. For this, wildtype (W1118) and Hsp70 deficient (Hsp70null) Drosophila melanogaster were subjected to simulated hypergravity at increasing levels of g-force (1.2g, 3g, and 5g) for acute (1hr) and chronic (7-day) timepoints and were compared to 0g 'non-hypergravity' controls. Following simulation, whole bodies were sex-segregated, RNA was isolated and quantitative (q)PCR was performed to determine differential immune gene expression profiles. Further, functional output of hemocytes were assessed by a phagocytosis assay. Collectively, these studies evaluated the effects of Hsp70 in the context of immunity during acute and chronic hypergravity. Indeed, relevance for this work can directly translate to acute effects of launch/landing gravitational forces upon liftoff (~1.7g) and entry (~3.4g) that astronauts experience. In addition, the effects of chronic cellular stress is directly relevant to the immune health of astronauts on long duration missions, as well. Thus, as we approach the goal of returning to the Moon and landing the first humans on Mars, an evaluation of gravity as a continuum and the stress-inducing effects of altered gravity experienced during spaceflight on astronaut immunity and health are necessary.

Description: Extra-terrestrial colonization is of growing interest to space agencies and private entities, emphasizing the importance of research on reproduction and development in the absence of Earth's 1G. Maternal stressors can modify offspring development, exerting significant lifespan and crossgenerational changes through prenatal programming. The space environment is stressful, therefore exposure to altered gravity during pregnancy may impact later life outcomes in offspring. In ground-based studies, we exposed pregnant rats to continuous +G (above Earth gravity), and observed overweight and elevated anxiety in adult male (but not female) offspring, common phenotypes associated with prenatal maternal stress. Here we hypothesize that exposure to increased gravity during pregnancy elicits changes in the expression of stress-related genes in placenta that may mediate emergence of later life outcomes. While the placenta transports maternal factors to the fetus and produces endogenous fetal hormones, stress-induced changes at the placental-uterine interface may also alter communication between mother and fetus, facilitating prenatal transmission of unfavorable later life outcomes and cross-generational epigenetic alterations. Maternal stress elevates maternal glucocorticoids however placental 11b-hydroxysteroid dehydrogenase type 2 (HSD11B2) buffers fetal exposure by converting cortisol/corticosterone into inactive metabolites. Maternal stress during pregnancy down-regulates this enzyme and can induce epigenetic changes in placental and fetal tissues accounting for heightened adult HPA reactivity. Past studies have shown a placenta-specific increase in DNA methyltransferase (DNMT3a) mRNA in stressed mothers, an effect with implications for genome-wide epigenetic changes that may account for diverse phenotypic outcomes following maternal stress. Here we exposed groups of pregnant rats to one of five gravity loads (1, 1.5, 1.75 and 2G) and analyzed placental samples during late gestation. We predicted a systematic dose-response relationship between gravity load and the expression of the HSD11B2 and DNMT3 genes, thereby linking maternal exposure to altered gravity during pregancy with maternal stress.

Description: Spaceflight has several detrimental effects on the physiology of astronauts, many of which are recapitulated in rodent models. We analyzed liver transcriptomic and proteomic data from three mouse spaceflight experiments flown aboard the International Space Station (Rodent Research-1 NASA (RR-1 NASA), Rodent Research-1 CASIS (RR-1 CASIS), Rodent Research-3 (RR-3)), and one mouse experiment flown on the Space Shuttle (Commercial Biomedical Testing Module-3 (CBTM-3) aboard STS-135). Despite the differences in genetic background and time of exposure to microgravity it was shown through Oil Red staining and histology that increased lipid accumulation was occurring in the liver of all mice flown in space compared to the ground controls. This led to further pursue the existing GeneLab datasets related to liver omics data from these mice. We were able to discover key conserved pathways across all the mice independent of the flight conditions that were related to increased lipid metabolism, fatty acid metabolism, both lipid and fatty acid processing, lipid catabolic processing, and lipid localization. In addition, key upstream regulators were predicted to be commonly regulated across all conditions which include ESR1, GCG, and NR1I2 being inhibited and INS being activated. Interestingly, estrogen receptor alpha (ESR1) expression has been known to be heavily involved with lipoprotein metabolism. In addition, insulin (INS) is the primary driver for fat metabolism and increased INS has been associated with increased fatty acids in the liver. Through additional proteomic analysis we were able to identify the majority of the key proteins related to lipids for both the RR-1 and RR-3 rodents were being up-regulated in the livers when comparing flight to ground controls. This additional confirmation of the lipid associated activity also showed that the lipid related proteins are heavily involved with lipid metabolism, cholesterol binding, and cholesterol metabolism. Lastly, the analysis also revealed that the circadian clock related pathways in the liver are commonly being increased across all space flight conditions which has also been reported in the literature to potentially cause increased liver damage. The combination of the very strong lipid uptake in the liver and the transcriptomic/proteomic signatures (including the circadian clock pathways) following spaceflight are consistent with early onset of liver disease. Taken together, these data indicate that, activation of lipotoxic pathways could persist during longer duration spaceflight which might result in the development of liver disease

Description: In-situ food production is a necessary step for human exploration of the solar system and requires a deep understanding of plant growth in reduced gravity environments. In particular, the lack of buoyancy-driven convection changes the gas exchange at the leaf surface, which decreases photosynthesis and transpiration rates, and ultimately biomass production. To understand the intricate relations between physical, chemical, and biochemical processes, the following methodology combines the development of a mechanistic model of plant growth in reduced gravity environments, computational fluid dynamics (CFD) simulations, and experiments in different time frames.The model presented here is a coupled mass and energy balance using the single round leaf assumption, including gravity as an entry parameter, and the leaf surface temperature as an output variable. Measures of the leaf surface temperature using infra-red cameras allow for a computation of the transpiration rate. This approach was followed to design a parabolic flight experiment, which performed 7 flights, and enabled data collection for model validation in different gravity and ventilation settings on a short time frame. Current measures of carbon assimilation and transpiration rate at the leaf and canopy level using an infra-red gas analyzer (Li-6800) in 1g lab conditions on several species will enable a validation on longer time frames and further calibration of the model. CFD studies both on the parabolic flight and on the lab experimental set-up allow the precise assessment of ventilation above the canopy and plants' leaves.Ultimately, this work will provide recommendations for the design of future plant growth hardware, especially on the lowest adequate ventilation for optimal plant growth in reduced gravity environments, as well as assessing biomass and oxygen production rates on planetary surfaces and space stations. This work was funded by CNES, CNRS, Clermont Auvergne Metropole, and NASA Space Biology through NASA postdoctoral program / USRA.

Description: Research on human acclimation to spaceflight, including the recent NASA's Twin Study, reports complex effects of the spaceflight environment on health, with both acute and prolonged changes in multiple tissues. Spaceflight includes multiple factors such as microgravity, ionizing radiation, physiological stress, and disrupted circadian rhythms, that have been shown to contribute to pathophysiological responses that target immunity, bone and muscle integrity, cardiovascular and nervous systems. In this study, we used a well-established spaceflight model organism, Drosophila melanogaster, to assess spaceflight-associated changes on the nervous system. With 75% disease gene orthology to humans, short generation time, large sample size and ease of genetic, neuronal and behavioral studies, Drosophila is an excellent model to study nervous system dysfunction. Here, we present results from MVP-Fly-01 spaceflight mission that was launched on SpaceX CRS-14. The MVP hardware (developed by Techshot) used in this mission enabled us to have an in-flight 1g centrifuge, to distinguish the changes resulting from gravity versus those induced by other environmental factors associated with spaceflight. We observe behavioral impairments (p〈0.001) and synaptic deficits, including decreased synaptic connections (p〈0.05), in 3rd instar larvae which were developed in space. Furthermore, space-grown microgravity adults show a decrease in neuronal (p〈0.05) and dendritic field (p〈0.01) in adult brains coupled with an increased number of apoptotic cells (p〈0.001) compared to in-flight 1g controls, suggesting increased neuronal loss under spaceflight conditions. In summary, we observe that altered gravity leads to gross neurological deficits. To better understand the long-term effects of spaceflight on the nervous system, longitudinal and multigenerational changes were also identified. This study will help elucidate the different approaches to prevent nervous system dysfunction in astronauts during spaceflight, while also contributing to a better understanding of the pathways that are related to some CNS disorders on Earth.

Description: Spaceflight poses many challenges for humans. Ground-based analogs typically focus on single parameters of spaceflight and their associated acute effects. This study assesses the long-term transcriptional effects following single and combination spaceflight analog conditions using the mouse model, simulated microgravity via hindlimb unloading (HLU) and/or low-dose irradiation (LDR) for 21-days, followed by 4 months of readaptation. Changes in gene expression and epigenetic modifications in whole brain samples during readaptation were analyzed by DESeq2 and reduced representation bisulfite sequencing (RRBS). The results showed minimal gene expression alterations at 4-months within single treatment conditions of HLU and LDR. Following combined HLU+LDR, gene ontology and methylation analyses showed multiple altered pathways involved in neurogenesis and neuroplasticity, regulation of neuropeptides and cellular signaling. In brief, neurological readaptation following combined chronic LDR and HLU is a dynamic process that impacts brain structure and function and may lead to late onset neurological sequelae

Description: The Advanced Plant Habitat (APH) was installed on the International Space Station (ISS) in October 2017. Following a successful EVT (Experiment Verification Test) study at Kennedy Space Center (KSC), using Arabidopsis lines with varying levels of lignin, two inaugural studies were carried out on ISS in 2018 under the same experimental design, with the corresponding ground controls at KSC. The APH for this study deploys a substrate-based root module designed for plant growth in microgravity. Upon experiment initiation (such as for the EVT), the root module is primed (liquid imbibition) by flooding the root zone to initiate seed germination and to remove air from the porous tubing and particulate media. In the APH ISS inaugural study, the speed of supplying water to initially dry media was found to adversely affect the overall moisture distribution within the root module in microgravity (but not at 1g). Non-destructive estimations of Arabidopsis plant growth were carried out by monitoring changes in rosette leaf area on a daily basis. These data indicated that the original priming procedure caused patchy moisture distribution that affected plant growth and survival. An improved methodology for priming the second root module of PH-01 was devised and implemented in the second experiment. Leaf area and color estimates suggested that the modified priming scheme improved moisture distribution and plant growth. These data, when compared with the EVT study, suggest that nondestructive measurements of plant growth can aid towards optimization of plant growth conditions in microgravity.

Description: This presentation is a summary of the continuing effort to determine options for studying artificial gravity with rodents. Results of an engineering trade study are presented and an overview of past and planned short radius centrifugation studies are presented. A leading proposal for a future flight centrifuge capable of housing rodents, the Techshot RCF, is presented in only enough detail as is approved by Techshot for public domain use.

Description: GeneLab must establish data processing pipelines for common data types including microarray, RNA-sequencing, and metagenomic profiling. Here we give an overview of current microarray and RNA-seq pipelines and discuss future pipelines including metagenomic profiling pipelines

Description: No abstract available

Description: Long-duration space missions will eventually require a fresh food supply to supplement crew diets, which means growing crops in space. The Passive Orbital Nutrient Delivery System (PONDS) is a new plant growth approach that contains both an area for a contained substrate and a reservoir for water and/or plant nutrient solutions. Ground studies have shown that the system facilitates both reliable water delivery to seeds for germination (e.g., while avoiding overwatering), and transport of water from the reservoir for improved plant growth while providing nutrients and oxygen to the root zone. In ground prototypes a capillary mat wicking material passively links the water/nutrient solution reservoir to a removable rooting module containing a substrate adapted to support plant growth. Oxygen permeable membranes are incorporated into both the reservoir walls and the rooting modules, bringing in oxygen from outside of the system into the reservoir and then into the rooting modules where the plant roots proliferate. Water is delivered from the reservoir to the substrate contained within the rooting module through the use of wicking material inserted into the plant growth substrate both from the bottom and from the sides of the rooting module. The capillary mat material is intrinsically hydrophilic and continuously wicks water to the substrate throughout the plant growth interval. The system is therefore self-watering in terms of supplying water to the root zone encompassed within the rooting module on demand. At the top, a hydrophilic phenolic foam plug surrounds the wick in the seed insertion zone, and both contains the substrate within the rooting module, and facilitates removal of excess moisture from the capillary mat wick before it can encompass seeds prior to germination. This work is supported by NASAs Space Life and Physical Sciences and Research Applications Division (SLPSRAD).

Description: For over 100 years, neurologists have used eye movements to identify neural impairment, disease, or injury. Prior to the age of modern imaging, qualitative assessment of eye movements was a critical, routine component of diagnosis and remains today a routine law-enforcement tool for detecting impaired driving due to drugs or alcohol. We will describe the application of a simple 5-minute oculomotor tracking task coupled with a broad range of quantitative analyses of high-resolution oculomotor measurements for the sensitive detection of sub-clinical neural impairment and for the potential differentiation of various causes. Specifically, we will show that there are distinct patterns of impairment across our set of oculometric parameters observed with brain trauma, sleep and circadian disruption, and alcohol consumption. Such differences could form the basis of a self-administered medical monitoring or diagnostic support tool.

Description: Long-term planetary space missions present new and unique challenges in life-support systems. Water constitutes the majority of the mass required to sustain human life in space and it follows that efficient water recycling has the potential to lower mission costs. The effect of partial gravity in planetary missions mean that terrestrial systems could be applicable. This trade study evaluates terrestrial and NASA developed water recycling technologies on the basis of applicability as a planetary base water recycling systems. Various bioreactors, membrane reactors, filtration, and district water reclamation systems are investigated and rated based on several standardized parameters. A customer-oriented Quality Function Deployment (QFD) is utilized to analyze the ratings of the technologies for the tasks required. The trade study aims to rank the various systems based on their Equivalent System Mass (ESM), Technology Readiness Level (TRL), scalability, crew time, and overall logistics requirements, among others. The results of the study can serve as a basis for future inquiries and studies by NASA and other interested parties. The results of this study provide a down selection from 24 systems to 5 systems that trade very close to each other. The results provide a context and justification for a future comparative hardware test program to determine which of these systems offer the best solution.

Description: As NASA's effort to establish a permanent residence in space continues, research on the effects of microgravity onbiological microorganisms is vital to protect or promote the health of plants and their astronaut counterparts. The purpose of this study is to determine the effects of microgravity on Pantoea agglomerans (P. agglomerans), using an analog microgravity simulator; the Rotary Cell Culture System (RCCS) developed at Johnson Space Center (JSC) in Houston, TX. P. agglomerans has been shown to be a plant growth promoter (PGPR) in ground based studies, but has also been shown to be a pathogen in both plants and immunocompromised patients. In this study, we will determine changes in the growth rate and antibiotic susceptibility of P. agglomerans when exposed to simulated microgravity.

Description: Use of a Sabatier reactor to recover the oxygen from the carbon dioxide exhaled by the crew on the International Space Station has been limited by the loss of the hydrogen contained in the methane it generates. Maximizing the oxygen recovered requires the hydrogen to be recovered from the methane product and recycled back to the Sabatier reactor. We describe the use of a tailored methane pyrolysis reactor to completely recover this hydrogen. The carbon-containing byproduct is elemental carbon, which is generated in the form of easily handled, non-sooty material that may have various uses. The process of creating this tailored carbon vapor deposition process involved exploration of the effects of temperature, pressure, substrate design and other variables to develop a high yield process that cleanly generates the desired products. Reaction kinetics and kinetics modelling were used to specify the temperature, pressure and reactor volume required to achieve the target conversion and to assure that the final average density was as high as possible. Reactor design included the selection of materials that will survive the high temperatures and environment in the pyrolysis reactor, and thermal modeling to achieve the required temperatures with minimum power consumption. The successful construction and demonstration of a brassboard prototype will allow the results of the chemical, thermal and mechanical models to be validated and should provide a useful alternative for a completely closed loop ECLS system. Integration of this technology with state-of-the-art (SOA) Sabatier hardware on ISS requires a complete understanding of the effects of impurities in the product hydrogen on the Sabatier catalyst. SOA Sabatier catalyst was evaluated over short and long-term exposure to anticipated contaminants to identify effects.

Description: The NASA Docking System (NDS) is a 31.4961-inch (800 mm) diameter circular hatch for astronauts to pass through when docked to other pressurized elements in space or for entrance or egress on surface environments. The NDS is utilized on the Orion Spacecraft and has been implemented as the International Docking System Standard (IDSS). The EV74 Human Factors Engineering (HFE) Team at NASAs Marshall Space Flight Center (MSFC) conducted human factors analyses with various hatch shapes and sizes to accommodate for all astronaut anthropometries and daily task comfort. It is believed that the hatch, approximately 32 inches, is too small, and a bigger hatch size would better accommodate most astronauts. In order to conduct human factors analyses, four participants were gathered based on anthropometry percentiles: 1st female, 5th female, 95th male, and 99th male.

Description: No abstract available

Description: Despite their numerical abundance and economic value, the behavior of many small coastal sharks in the US South Atlantic has been only coarsely described. Here we present movement summaries for blacknose (Carcharhinus acronotus), finetooth (C. isodon), and Atlantic sharpnose shark (Rhizoprionodon terraenovae) as they travelled through a regional-scale acoustic telemetry network, offering direct comparisons of habitat utilization, site fidelity, and the extent and timing of coastal migrations. From 2013-2016, 165 total sharks were implanted with acoustic transmitters at Cape Canaveral, Florida, and tracked up to four years. While blacknose sharks were common off east Florida year-round, finetooth sharks were most abundant winter through early spring and sharpnose sharks summer through fall. Blacknose sharks also moved more slowly (mean 0.8 kilometers per hour) and had the broadest depth preferences, while finetooth sharks were strongly shore-associated and sharpnose preferred proportionally deeper waters. All species exhibited low site fidelity when at Cape Canaveral, remaining at the same site for more than 1 hour on average, even when associated with deeper hard-bottom sites. Most finetooth and many blacknose undertook spring migrations as far as Virginia and North Carolina, respectively, before returning to east Florida each winter. Sharpnose also made regular northward movements that were not as obviously seasonally-driven. Multiple individuals of all species, particularly females, returned briefly south to Cape Canaveral in mid-summer, illustrating that coastal migrations in these species are more akin to seasonal expansions of their geographic ranges as opposed to a synchronized shift of the entire population along the coast.

Description: The rodent hindlimb unloading (HU) model was initially developed to simulate the cephalad fluid shift and musculoskeletal disuse in astronauts. Since then, the HU model has been applied to explore how other systems (e.g. immune, cardiovascular and CNS) respond to weightlessness. Most HU studies are performed with singly-housed animals, although social isolation also can substantially impact behavior and physiology, and therefore may confound HU experimental results. We hypothesized that relative to social housing, single housing exacerbates HU-induced dysfunction in select organ systems. We refined the standard NASA-Ames HU model to accommodate social housing in HU pairs, retaining advantageous features of traditional housing but using commercial off-the-shelf components to facilitate adoption by others. We conducted a 30 day HU experiment with adult, female C57Bl6/NJ mice that were either singly or socially housed. HU animals in both single and social HU housing displayed expected musculoskeletal deficits compared to housing matched, normally loaded (NL) controls. However, select immune, HPA axis, and CNS responses were differentially impacted by the HU social environment relative to NL controls. HU reduced % CD4+ T cells in singly-housed, but not socially-housed mice. Surprisingly, HU increased adrenal gland mass in socially-housed but not singly-housed mice, while social isolation increased adrenal gland mass in NL controls. HU also increased plasma corticosterone levels (day 30) in both singly and socially-housed mice. Thus, the social environment altered select adrenal and immune, but not musculoskeletal, responses to simulated weightlessness. We refine our original hypothesis since our results show combined stressors can mask, not only exacerbate, tissue responses to HU. These findings further expand the utility of the HU model for studying possible combined effects of the various spaceflight stressors.

Description: The effects of microgravity, and social isolation on the CNS are poorly understood. We hypothesize that mitochondrial reactive oxygen species (ROS) play an important role in this process. Since mice are social animals, our lab developed a novel social model of hindlimb unloading (HU), enabling us to determine the effects of both social isolation and simulated microgravity. Responses to 30d of HU were compared in wildtype or transgenic MCAT mice who over-express human catalase in mitochondria. Abundance of 4-Hydroxynonenal, Park7 (a redox-sensitive chaperone and sensor of oxidative stress) and corticosterone were measured by ELISA. Cytokines related to inflammation in the hippocampus and in plasma were analyzed by a protein array. Behavioral data was collected over a 24-hour period.Socially housed HU mice were more active and conducted at least two times more exploratory activities, compared to normally loaded mice. Correlation analysis revealed that specific brain and plasma cytokines correspond with specific behaviors. Simulated microgravity and/or social isolation caused changes in cytokine patterns in the hippocampus and in plasma, with significant interaction effects of HU and genotype in expression levels of five cytokines (out of 35). Interestingly, elevation of these generally pro-inflammatory cytokines by HU in WT mice was mitigated in MCAT mice, suggesting a role for mitochondrial ROS signaling in inflammatory CNS responses to microgravity. Interestingly, socially housed mice had also lower level of 4HNE and higher level of Park7 in the hippocampus compared to singly housed animals. The cytokine responses to social isolation were more extensive in brain vs plasma. Further, there was no overlap in the cytokine repertoire regulated in response to microgravity versus, isolation suggesting divergent mechanisms or downstream signaling. These findings implicate a potentially important role for mitochondrial ROS in CNS responses to the challenges posed both by prolonged missions in space and bedrest on Earth

Description: Spaceflight and the ensuing fluid shifts, together with an overall reduction in physical activity, lead to acute and latent effects on the cardiovascular system. This current study makes use of the rodent hindlimb unloading (HU) model to determine how factors such as sex, age, and duration of exposure impact cardiac responses to weightlessness. We hypothesize that extended exposure to simulated weightlessness and the ensuing recovery alters cardiac structure and expression of select genes, including those involved in redox signaling which together, negatively impact long-term cardiac tissue health. To begin to test this hypothesis, male and female rats underwent HU at various durations up to 90 days, with a subset reambulated after 90 days of HU. Physiological stress or contractility changes lead to alterations in ventricular cardiomyocyte size and ventricular wall thickness to adapt to greater functional demand and mitigate mechanical stress to ventricular tissue; under certain conditions, these changes also may mark progression to cardiac failure. Hence, left ventricular cardiomyocyte size (cardiomyocyte cross sectional area, CSA) was quantified to determine if HU leads to structural adaptation responses in cardiac tissue and if age and sex had any impact on this outcome. Cardiomyocyte CSA of older males (9 months) were altered by HU in a time-dependent manner, where HU led to decreases in CSA at 14 days and increases at 90 days. In contrast, younger males (3 months) did not show any changes at day 14 of HU. CSA of females (3 months) was increased in response to short-term HU (14 days) suggesting sex-dependence of structural changes. In older HU males, cardiomyocyte CSA was comparable to controls after 90 days of re-ambulation. Levels of the DNA oxidative damage marker, 8-hydroxydeoxyguanosine (8-OHdG) were greater in left ventricular tissue of females that underwent HU compared to sex-matched controls, while there were no such differences in older or younger males. To gain insight into the signals that drive cardiac adaptations to HU, global transcriptomic analysis (RNAseq) was performed on left ventricular tissue of older males that underwent 14 days of HU. Short-term simulated weightlessness led to differential expression of genes involved in immune and pro-inflammatory signaling. A subset of these genes play a role in autoimmune and cardiovascular disease and are targets of current drugs used to treat bradycardia, hypertension, atherosclerosis and rheumatoid arthritis, amongst others. Oxidative damage/redox signaling pathways were not enriched at the timepoint tested in older males. Since young females displayed greater oxidative damage to DNA, activation of oxidative stress responses at earlier or later time points cannot be ruled out. In summary, simulated weightlessness in adult rats caused changes in cardiomyocyte structure in a sex and age-dependent manner, and the transcriptional regulation of key mediators of immunity and cardiovascular disease, meriting further study to define cardiac risks for interplanetary travel of human crew. Our findings also confirm the value of the rat HU model for cardiac health and countermeasure research.

Description: Future long-duration missions face significant challenges maintaining crew health. A critical area is supplying adequate nutrition, as certain vitamins and nutrients in supplied foods and supplements demonstrate substantial degradation during extended storage. To address this issue, we are developing and flight-testing a platform technology that demonstrates in situ microbial production of targeted nutrients over extended mission durations. This 5-year experiment, known as BioNutrients-1, was started on the International Space Station in May 2019. It involves two components: an on-orbit hydration and production experiment; and the development of space-compatible, key bio-manufacturing microorganisms. On-orbit testing utilizes a small production pack system that encloses sterile edible growth substrate and desiccated Saccharomyces cerevisiae strains genetically engineered to produce the nutrients beta-carotene or zeaxanthin. On hydration and mixing of the production pack, the organisms revive and grow until limited by the depletion of growth media, hypothetically leading to consistent amounts of biomass and nutrients. In eventual mission applications, the packet contents would be heat treated to inactivate the microorganisms prior to consumption. For these flight experiments, the packet will not be heat treated, but will instead be frozen for return to Earth for analyses. In addition to the production pack trials, 14 different microorganisms/treatments were also delivered to ISS for long-duration storage. These samples will be intermittently returned to Earth and analyzed to determine survival rates and genomics. For this presentation, initial data from returned samples and ground controls will be discussed.

Description: Plant associated microbiomes, the rhizosphere and phyllosphere, are composed of communities of bacteria and fungi that may be mutualistic or pathogenic. These communities have the potential to influence plant health and development and can affect plant growth. Crop plants are being investigated as a fresh and safe supplement to astronauts diet and it is critical to understand and characterize these microbial communities. Multi-species crops, Mizuna mustard (Brassica rapa var japonica), Outredgeous red romaine lettuce (Lactuca sativa), and Waldmans Green lettuce (Lactuca sativa) were grown in two Veggie units on the International Space Station (ISS) for three grow outs in various combinations of plant types. Upon harvest, plant and pillow samples were frozen and returned to Earth for analysis. Bacterial and fungal community analyses for plant leaf and root, as well as pillow components, wick and media, were completed using next generation sequencing with the goal of surveying the composition of the entire community and identifying any potential pathogens. Bacteria were identified using the 16S rRNA gene whereas, fungi were identified with the internal transcribed spacer (ITS). The community composition for these three crops was compared between crop types and between plant tissue types. It is vital to mission success for the short term and long term to add nutritious, safe to eat vegetables providing a supplement to the crew members dietary requirements as well as to develop planning for deep space missions as we reach for the moon and on to Mars. Veggie technology validation tests were supported by NASAs Space Biology Program.

Description: It is important to determine the health risks and potential survival for astronauts associated with long-term space missions. This entails not only understanding the impact the space environment will have on humans, but also how it will affect other organisms needed for humans to survive in space such as plants. In addition, it has been reported in the literature that hundreds of genes seem to be conserved and/or transferred between different organisms from bacteria, archaea, fungi, microorganisms, and plants to animals. Since space travel involves humans in a closed environment over a long period of time, we hypothesize that potential conserved biological factors will occur between the different organisms in that environment possibly due to transfer of genes. Determining the conserved factors that are commonly being regulated in space can shed insight into possible universal master regulators and also determine the symbiotic relationship between the organisms in space. Utilizing NASA's GeneLab Data Repository (a rapidly expanding, curated clustering of spaceflight-related omics-level datasets for all organisms), we were able to uncover a novel pathway and factors that were commonly shared between humans, mice, plants, C. Elegans, and drosophilas. Through ChIP-Seq enrichment analysis techniques utilizing various GeneLab datasets from each species that were flown in space, we found the following factors to be conserved across all species: oxidative stress, DNA damage (through GABPA/NRFs and NFY), SIX5, GTF2B and glutamine synthetase. Such commonalities would likely reflect the effects of factors such as microgravity and the increased radiation exposure inherent in spaceflight on basic physical processes shared by all biological systems at the cellular level. Differences between organismal responses revealed by GeneLab's data should also help understand the unique reactions to life in space that arise from the very different lifestyles of microbes, animals and plants.

Description: A comprehensive understanding of the effects of spaceflight and altered gravity on human physiology is necessary for continued human space exploration and long-term space habitation. The oxidative stress response has been identified in astronauts exposed to short- and long-term space missions that are exposed to the multitude of stress factors of spaceflight, including altered gravity and radiation exposure. Reactive oxygen species (ROS) are byproducts of homeostatic cellular metabolism, yet when overproduced the oxidative stress response ensues, rendering molecules destructive causing cell death and inflammation. Controlling aberrant ROS production is necessary to prevent pathological consequences, in particular within the nervous system, since neurons are extremely sensitive overexpressed ROS insults. We hypothesize that exposure to altered gravity triggers the oxidative stress response, leading to impairments in the nervous system. In this study, we used a well-established spaceflight model organism, Drosophila melanogaster, to assess altered gravity associated changes in the nervous system using a ground-based hypergravity model. Acute hypergravity resulted in an induction of oxidative stress-related genes with an increase in reactive oxygen species (ROS) in fly brains (p〈0.001). Also, qPCR analysis shows that parkin gene expression is significantly reduced in these fly brains(p〈0.05). Additionally, chronic hypergravity resulted in depressed locomotor phenotype in these flies (p〈0.05) in conjunction to decreased dopaminergic neuron counts (p〈0.0001) and increased apoptosis in these fly brains (p〈0.0001). Further, assessment of neurological changes, including the neuronal architecture, synaptic integrity and genetic regulation caused by hypergravity conditions were noted. Overall, our results validate chronic hypergravity simulation as a behavioral model to study spaceflight effects, and oxidative stress pathway as a potential avenue for countermeasure development for astronauts undergoing short- and long-term missions and for neurodegenerative research on Earth.

Description: With humans pushing to live further off Earth for longer periods of time, it is increasingly important to understand the changes that occur in biological systems during spaceflight whether these be astronauts, their microbial commensals, or their plant-based life support systems. In a three-part presentation, we discuss GeneLab and recent discoveries regarding the microbiota of spacecrafts and space-flown animals. Part 1: GeneLab: Open Science for Life in Space, Jonathan Galazka, NASA Ames Research Center To accelerate the pace of discovery from precious spaceflight biological experiments, NASA as develop the GeneLab data system (genelab.nasa.gov), which allows unfettered access to omics data from spaceflight and spaceflight relevant experiments. GeneLab houses metagenomic datasets from spacecraft and relevant spacecraft models. Users can download this data and associated metadata to make new discoveries about how microbial communities may change and adapt to spaceflight.

Description: High-LET ionizing radiation is a major occupational health hazard for astronauts, but risk assessment remains elusive due to limited epidemiological data. Identifying genetic factors modulating the individual radiation response may be the most effective strategy to provide individualized risk management for long-duration high-radiation missions. We have started tackling the challenge of predicting individual risks by identifying human genetic loci associated with various radiation sensitivity phenotypes in primary blood mononuclear cells from a relatively large healthy human cohort. To date, we have performed the isolation of PBMCs from 768 subjects of the same ethnicity, and irradiated PBMCs from 576 subjects with 1 and 3 particles/100m2 of 600 MeV/n 56Fe, 350 MeV/n 40Ar and 350 MeV/n 28Si ions. The phenotypes of interest were: number of radiation-induced foci (or RIFs), CellROX oxidative stress responses and cell death, at 4h and 24h following irradiation. We have observed a significant inter-individual variability at 0 Gy between the 576 studied subjects, with a mean fold difference between the 10% lowest and highest responders of 5.6 of RIFs/cell, 7.9 in mean CellRox intensity, and 9.3 in percentage of dead cells. In order to better assess genetic factors influencing DNA repair, we used a metric previously introduced by our group to sort out radiation sensitivity phenotypes in mice: i.e. the ratio of the first to the second slope of RIFs/cell (between 0 and 1, and between 1 and 3 particle/100m2). Preliminary data on 192 individuals showed a distribution of low-dose responders (ratio 〉 1) to high-dose responders (ratio 〈 1) at 4h of 12%, 55% and 52% respectively for Fe, Ar and Si. The average value for the first and the second slopes was very similar for the two lowest LET (0.10 [-0.26;0.58] and 0.09 [-0.45;0.41] for Ar, 0.07 [-0.27;0.38] and 0.08 [-0.19;0.42] for Si), indicating a linear dose response across both fluence. Fe showed clear saturation for the highest dose with a slope of -0.09 [-0.86;1.51] against 0.68 [-2.21;2.20] for the low dose range, which probably reflects that many PBMCs are beyond repair at the high dose. Note that other significances were found for additional factors such as BMI and age whereas none were found for sex. GWAS will be performed on all phenotypes upon completion of measurements.

Description: The NASA GeneLab project capitalizes on multi-omic technologies to maximize the return on spaceflight experiments. To do this, GeneLab maintains a publicly accessible database (GLDS) that houses spaceflight and spaceflight relevant multi-omics data, and collaborates with NASA principal investigators and projects to generate additional omics data. GeneLab houses more than 200 transcriptomic, proteomic, metabolomic and epigenomic datasets from plant, animal and microbial experiments, with a growing number of these having been produced by the GeneLab sample processing lab. The GLDS contains rich metadata about each experiment and has recently integrated radiation dosimetery data from experiments flown on the Space Shuttle. GeneLab has also recently implemented an effort to present processed data in the GLDS in addition to the raw omics data. The processed data will enable interpretation of the data by a larger group of students, scientists and the general public. Standard pipelines for the transformation of raw data into visualizations were developed by four GeneLab Analysis Working Groups (animals, plants, microbes, multi-omics) comprised of over 100 scientists from NASA and academia. These pipelines are now being used by a group of bioinformatics interns to provide standard basic analysis of the data for incorporation into GLDS.

Description: Spaceflight can cause immune system dysfunction, such as elevated white blood cells (WBC) and polymorphonuclear neutrophils (PMN), along with unchanged or reduced lymphocyte counts. A high PMN to lymphocyte ratio (NLR) can acts as a poor prognosis in cancer and a biomarker for subclinical inflammation however, the NLR has not been identified as a predictor of astronaut health during spaceflight. CBC data collected on board the International Space Station (ISS) was repurposed to determine the granulocyte to lymphocyte ratio (GLR) in humans and the NLR in rodents. The results displayed a progressive increase in GLR and NLR during spaceflight and at landing. The mechanism for increased NLR was assessed in vitro using the microgravity-analog, rotating wall vessel (RWV), with human WBCs. The results indicated that simulated microgravity led to increased GLR and NLR profiles, and production of reactive oxygen species (ROS) and myeloperoxidase (MPO). Interestingly, simulated microgravity increased the number of matured PMNs that showed impaired phagocytic function, while treatment with tert-Butyl hydroperoxide (TBHP), also reduced PMN phagocytosis. In addition, 30-days of simulated microgravity (hindlimb unloading) in mice, indicated an increased NLR and MPO gene expression, which were mitigated in mitochondrial catalase overexpressing transgenic mice, suggesting ROS scavenging is essential for maintaining homeostatic immunity. Collectively, we propose that the health status of astronauts during future short- and long-term space missions can be monitored by their NLR profile, in addition to utilizing this measurement as a tool for oxidative stress response countermeasure development to restore homeostatic immunity.

Description: As next-generation space exploration missions necessitate increasingly autonomous systems, there is a critical need to better detect and anticipate crewmember interactions with these systems. The success of present and future autonomous technology in exploration spaceflight is ultimately dependent upon safe and efficient interaction with the human operator. Optimal interaction is particularly important for surface missions during highly coordinated extravehicular activity (EVA), which consists of high physical and cognitive demands with limited ground support. Crew functional state may be affected by a number of variables including workload, stress, and motivation. Real-time assessments of crew state that do not require a crewmembers time and attention to complete will be especially important to assess operational performance and behavioral health during flight. In response to the need for objective, passive assessment of crew state, the aim of this work is to develop an accurate and precise prediction model of human functional state for surface EVA using multi-modal psychophysiological sensing. The psychophysiological monitoring approach relies on extracting a set of features from physiological signals and using these features to classify an operators cognitive state. This work aims to compile a non-invasive sensor suite to collect physiological data in real-time. Training data during cognitive and more complex functional tasks will be used to develop a classifier to discriminate high and low cognitive workload crew states. The classifier will then be tested in an operationally relevant EVA simulation to predict cognitive workload over time. Once a crew state is determined, further research into specific countermeasures, such as decision support systems, would be necessary to optimize the automation and improve crew state and operational performance.

Description: No abstract available

Description: The presentation covers two recent studies Lunar In Situ Resource Utilization (ISRU) systems to produce propellant for an early reusable lander architecture. The first study examines the hardware, power, and operations required to produce 10 metric tons of oxygen per year near the lunar south pole using the Carbothermal Reduction process. The second study examines the hardware, power, and operations to mine and process 15 metric tons of water from a permanently shadowed crater near Shackleton crater.