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  • American Association for the Advancement of Science (AAAS)
  • 2000-2004  (1,572)
  • 1995-1999
  • 1970-1974
  • 2000  (1,572)
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  • 2000-2004  (1,572)
  • 1995-1999
  • 1970-1974
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  • 1
    Publication Date: 2000-01-05
    Description: High-precision genetic mapping was used to define the regions that contain centromere functions on each natural chromosome in Arabidopsis thaliana. These regions exhibited dramatic recombinational repression and contained complex DNA surrounding large arrays of 180-base pair repeats. Unexpectedly, the DNA within the centromeres was not merely structural but also encoded several expressed genes. The regions flanking the centromeres were densely populated by repetitive elements yet experienced normal levels of recombination. The genetically defined centromeres were well conserved among Arabidopsis ecotypes but displayed limited sequence homology between different chromosomes, excluding repetitive DNA. This investigation provides a platform for dissecting the role of individual sequences in centromeres in higher eukaryotes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Copenhaver, G P -- Nickel, K -- Kuromori, T -- Benito, M I -- Kaul, S -- Lin, X -- Bevan, M -- Murphy, G -- Harris, B -- Parnell, L D -- McCombie, W R -- Martienssen, R A -- Marra, M -- Preuss, D -- New York, N.Y. -- Science. 1999 Dec 24;286(5449):2468-74.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉University of Chicago, Department of Molecular Genetics and Cell Biology, 1103 East 57 Street, Chicago, IL 60637, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10617454" target="_blank"〉PubMed〈/a〉
    Keywords: Arabidopsis/chemistry/*genetics ; Base Composition ; Base Sequence ; Centromere/*genetics/physiology ; Conserved Sequence ; Contig Mapping ; Crosses, Genetic ; Crossing Over, Genetic ; DNA, Plant/chemistry/*genetics ; Gene Expression ; *Genes, Plant ; Meiosis ; Models, Genetic ; *Recombination, Genetic ; *Repetitive Sequences, Nucleic Acid ; Retroelements ; Sequence Analysis, DNA
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    Electronic ISSN: 1095-9203
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  • 2
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-01-15
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hardin, P E -- Glossop, N R -- New York, N.Y. -- Science. 1999 Dec 24;286(5449):2460-1.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biology, University of Houston, Houston, TX 77204, USA. phardin@uh.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10636810" target="_blank"〉PubMed〈/a〉
    Keywords: ARNTL Transcription Factors ; Animals ; Basic Helix-Loop-Helix Transcription Factors ; Biological Clocks/*physiology ; CLOCK Proteins ; Cell Cycle Proteins ; Circadian Rhythm/*physiology ; Cryptochromes ; Darkness ; Drosophila ; *Drosophila Proteins ; *Eye Proteins ; Feedback ; Flavoproteins/genetics/*physiology ; Gene Expression Regulation ; Light ; Mice ; Mice, Knockout ; Nuclear Proteins/*genetics/metabolism ; Period Circadian Proteins ; *Photoreceptor Cells, Invertebrate ; Promoter Regions, Genetic ; Receptors, G-Protein-Coupled ; Repressor Proteins/genetics/physiology ; Suprachiasmatic Nucleus/metabolism ; Trans-Activators/physiology ; Transcription Factors/physiology ; *Transcription, Genetic
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  • 3
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-01-05
    Description: The assumption that human mitochondrial DNA is inherited from one parent only and therefore does not recombine is questionable. Linkage disequilibrium in human and chimpanzee mitochondrial DNA declines as a function of the distance between sites. This pattern can be attributed to one mechanism only: recombination.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Awadalla, P -- Eyre-Walker, A -- Smith, J M -- New York, N.Y. -- Science. 1999 Dec 24;286(5449):2524-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 1JT, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10617471" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; DNA, Mitochondrial/*genetics ; Evolution, Molecular ; Fathers ; Female ; Hominidae/*genetics ; Humans ; *Linkage Disequilibrium ; Male ; NADH Dehydrogenase/genetics ; Pan troglodytes/*genetics ; Polymorphism, Restriction Fragment Length ; *Recombination, Genetic
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  • 4
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-01-05
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cyster, J G -- New York, N.Y. -- Science. 1999 Dec 10;286(5447):2098-102.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94143, USA. cyster@itsa.ucsf.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10617422" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; B-Lymphocytes/*immunology ; Cell Movement ; Chemokines/*immunology ; *Chemotaxis, Leukocyte ; Dendritic Cells/immunology ; Humans ; Immunologic Memory ; Ligands ; Lymphocyte Activation ; Lymphoid Tissue/*immunology ; T-Lymphocytes/*immunology
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  • 5
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-01-15
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hathcock, J N -- New York, N.Y. -- Science. 1999 Dec 17;286(5448):2269; author reply 2270-1.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10636785" target="_blank"〉PubMed〈/a〉
    Keywords: Consumer Advocacy ; Consumer Product Safety/legislation & jurisprudence ; *Dietary Supplements ; Humans ; *Legislation, Drug ; *Legislation, Food ; Lobbying ; United States ; United States Food and Drug Administration
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  • 6
    Publication Date: 2000-01-05
    Description: Mice lacking mCry1 and mCry2 are behaviorally arrhythmic. As shown here, cyclic expression of the clock genes mPer1 and mPer2 (mammalian Period genes 1 and 2) in the suprachiasmatic nucleus and peripheral tissues is abolished and mPer1 and mPer2 mRNA levels are constitutively high. These findings indicate that the biological clock is eliminated in the absence of both mCRY1 and mCRY2 (mammalian cryptochromes 1 and 2) and support the idea that mammalian CRY proteins act in the negative limb of the circadian feedback loop. The mCry double-mutant mice retain the ability to have mPer1 and mPer2 expression induced by a brief light stimulus known to phase-shift the biological clock in wild-type animals. Thus, mCRY1 and mCRY2 are dispensable for light-induced phase shifting of the biological clock.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Okamura, H -- Miyake, S -- Sumi, Y -- Yamaguchi, S -- Yasui, A -- Muijtjens, M -- Hoeijmakers, J H -- van der Horst, G T -- New York, N.Y. -- Science. 1999 Dec 24;286(5449):2531-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Anatomy and Brain Science, Kobe University School of Medicine, Kobe 650-0017, Japan. okamurah@kobe-u.ac.jp〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10617474" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Biological Clocks/*physiology ; Cell Cycle Proteins ; Circadian Rhythm/*physiology ; Cryptochromes ; *Drosophila Proteins ; *Eye Proteins ; Feedback ; Flavoproteins/genetics/*physiology ; Gene Expression Regulation ; In Situ Hybridization ; *Light ; Liver/metabolism ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mutation ; Nuclear Proteins/*genetics ; Period Circadian Proteins ; *Photoreceptor Cells, Invertebrate ; Polymerase Chain Reaction ; RNA, Messenger/genetics/metabolism ; Receptors, G-Protein-Coupled ; Retina/metabolism ; Suprachiasmatic Nucleus/metabolism ; Transcription Factors
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  • 7
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-01-05
    Description: Nitrogen and argon isotopes were measured in carbonatites and associated rocks from the Kola Peninsula in Russia. The Kola mantle source, which is thought to be located in the deep mantle, is enriched in heavy nitrogen (+3 per mil relative to air) as compared to Earth's surface (atmosphere and crust, +2 per mil) and the shallow mantle (-4 per mil). Recycling of oceanic crust (+6 per mil) or metal-silicate partitioning may account for the nitrogen isotopic composition of the deep mantle.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dauphas -- Marty -- New York, N.Y. -- Science. 1999 Dec 24;286(5449):2488-90.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Centre de Recherches Petrographiques et Geochimiques, CNRS UPR 9046, 15 rue Notre-Dame des Pauvres, Boite Postale 20, 54501 Vandoeuvre-les-Nancy Cedex, France. Ecole Nationale Superieure de Geologie, rue du doyen Marcel Roubault, Boit.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10617459" target="_blank"〉PubMed〈/a〉
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  • 8
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-01-05
    Description: Ions typically pass with difficulty from water into organic phases because of water's superior solvation power. This inhibits such processes as ion transport in batteries or in lipid bilayers of cells. Ion penetration across such an interface was studied with unusual structural control. Hydronium ions were soft-landed at 1 electron volt on cold films of 3-methylpentane ("oil") on a metal substrate. The field produced by these ions drove them through the films when warmed. Coadsorption of water (0.14 to 35 bilayers) inhibited the ion penetration by creating a solvation energy trap. A Born solvation model successfully predicted the trapping energies (0 to 38 kilojoules per mole).〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wu -- Iedema -- Cowin -- New York, N.Y. -- Science. 1999 Dec 24;286(5449):2482-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Pacific Northwest National Laboratory, Box 999, Mail Stop K8-88, Richland, WA 99352, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10617457" target="_blank"〉PubMed〈/a〉
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  • 9
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-01-15
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sikorski, R -- Peters, R -- New York, N.Y. -- Science. 1999 Dec 17;286(5448):2293-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10636792" target="_blank"〉PubMed〈/a〉
    Keywords: Cell Separation/*instrumentation/methods ; Flow Cytometry/*instrumentation/methods ; *Silicone Elastomers
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  • 10
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-01-05
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wuethrich, B -- New York, N.Y. -- Science. 1999 Dec 10;286(5447):2054-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10617414" target="_blank"〉PubMed〈/a〉
    Keywords: Anthropology ; DNA, Mitochondrial/*genetics ; Genetic Variation ; *Genetics, Population ; Humans ; Language ; Melanesia ; Polynesia ; *Sequence Deletion
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  • 11
    Publication Date: 2000-01-05
    Description: The nematode pharynx has a potassium channel with unusual properties, which allows the muscles to repolarize quickly and with the proper delay. Here, the Caenorhabditis elegans exp-2 gene is shown to encode this channel. EXP-2 is a Kv-type (voltage-activated) potassium channel that has inward-rectifying properties resembling those of the structurally dissimilar human ether-a-go-go-related gene (HERG) channel. Null and gain-of-function mutations affect pharyngeal muscle excitability in ways that are consistent with the electrophysiological behavior of the channel, and thereby demonstrate a direct link between the kinetics of this unusual channel and behavior.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3791429/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3791429/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Davis, M W -- Fleischhauer, R -- Dent, J A -- Joho, R H -- Avery, L -- HL46154/HL/NHLBI NIH HHS/ -- NS28407/NS/NINDS NIH HHS/ -- R01 HL046154/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1999 Dec 24;286(5449):2501-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9148, USA. wdavis@biology.utah.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10617464" target="_blank"〉PubMed〈/a〉
    Keywords: Action Potentials ; Animals ; Caenorhabditis elegans/genetics/*physiology ; Feeding Behavior ; Genes, Helminth ; Genes, Reporter ; Ion Channel Gating ; Kinetics ; Membrane Potentials ; Models, Molecular ; Muscles/metabolism ; Mutation ; Neurons/metabolism ; Oocytes/metabolism ; Pharyngeal Muscles/physiology ; Potassium Channels/chemistry/genetics/*physiology ; Protein Conformation ; RNA, Complementary/genetics ; Recombinant Fusion Proteins/biosynthesis ; Xenopus laevis
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  • 12
    Publication Date: 2000-01-05
    Description: Phytochromes are a family of photoreceptors used by green plants to entrain their development to the light environment. The distribution of these chromoproteins has been expanded beyond photoautotrophs with the discovery of phytochrome-like proteins in the nonphotosynthetic eubacteria Deinococcus radiodurans and Pseudomonas aeruginosa. Like plant phytochromes, the D. radiodurans receptor covalently binds linear tetrapyrroles autocatalytically to generate a photochromic holoprotein. However, the attachment site is distinct, using a histidine to potentially form a Schiff base linkage. Sequence homology and mutational analysis suggest that D. radiodurans bacteriophytochrome functions as a light-regulated histidine kinase, which helps protect the bacterium from visible light.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Davis, S J -- Vener, A V -- Vierstra, R D -- New York, N.Y. -- Science. 1999 Dec 24;286(5449):2517-20.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Genetics, Cellular and Molecular Biology Program and Department of Horticulture, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI 53706, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10617469" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Amino Acid Substitution ; Bacterial Proteins/chemistry/genetics/*metabolism ; Biliverdine/analogs & derivatives/metabolism ; Binding Sites ; Gram-Positive Cocci/genetics/*metabolism ; Histidine/metabolism ; Light ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Photoreceptors, Microbial/chemistry/genetics/*metabolism ; Phytochrome/metabolism ; Protein Kinases/chemistry/genetics/*metabolism ; Pseudomonas aeruginosa/*metabolism ; Signal Transduction
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  • 13
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-01-15
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉De Greene, K B -- New York, N.Y. -- Science. 1999 Dec 24;286(5449):2449.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10636804" target="_blank"〉PubMed〈/a〉
    Keywords: *Bayes Theorem ; Risk Assessment ; Statistics as Topic
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  • 14
    Publication Date: 2000-01-15
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Henry, S H -- Bosch, F X -- Troxell, T C -- Bolger, P M -- New York, N.Y. -- Science. 1999 Dec 24;286(5449):2453-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Washington, DC 20204, USA. SHenry@bangate.fda.gov〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10636808" target="_blank"〉PubMed〈/a〉
    Keywords: Aflatoxins/*analysis/toxicity ; Animals ; Developing Countries ; Food/*standards ; Food Analysis ; Food Contamination/*prevention & control ; *Global Health ; Hepatitis B Vaccines ; Humans ; Immunization Programs ; *Legislation, Food ; Liver Neoplasms/epidemiology/etiology/*prevention & control ; Prevalence ; Public Health ; Public Policy ; Risk Assessment ; United Nations
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  • 15
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-01-15
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Barinaga, M -- New York, N.Y. -- Science. 1999 Dec 24;286(5449):2434-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10636797" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Biological Clocks/*physiology ; Brain/metabolism ; CLOCK Proteins ; Circadian Rhythm/*physiology ; Darkness ; Drosophila/genetics/physiology ; *Drosophila Proteins ; Gene Expression Regulation ; Genes, Insect ; Light ; Mutation ; Neurons/metabolism ; Neuropeptides/genetics/*physiology ; Transcription Factors/genetics/physiology
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  • 16
    Publication Date: 2000-01-05
    Description: Measurement of (231)Pa-(235)U disequilibrium in Tonga-Kermadec island arc lavas (north of New Zealand) permits discrimination of the time scales and mechanisms of fluid addition and partial melting. For Tonga lavas, the ((231)Pa/(235)U) ratios are less than 1 and can be explained by fluid addition from the subducting plate around 60,000 years ago. In contrast, the effects of partial melting overprinted those of fluid addition in the Kermadec lavas resulting in ((231)Pa/(235)U) ratios greater than 1.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bourdon -- Turner -- Allegre -- New York, N.Y. -- Science. 1999 Dec 24;286(5449):2491-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratoire de Geochimie et Cosmochimie, Institut de Physique du Globe de Paris-CNRS, UMR7579, 4 Place Jussieu, 75252 Paris Cedex 05, France. Department of Earth Sciences, The Open University, Milton Keynes MK7 6AA, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10617460" target="_blank"〉PubMed〈/a〉
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  • 17
    Publication Date: 2000-01-05
    Description: Angiopoietin-1 (Ang1) and vascular endothelial growth factor (VEGF) are endothelial cell-specific growth factors. Direct comparison of transgenic mice overexpressing these factors in the skin revealed that the VEGF-induced blood vessels were leaky, whereas those induced by Ang1 were nonleaky. Moreover, vessels in Ang1-overexpressing mice were resistant to leaks caused by inflammatory agents. Coexpression of Ang1 and VEGF had an additive effect on angiogenesis but resulted in leakage-resistant vessels typical of Ang1. Ang1 therefore may be useful for reducing microvascular leakage in diseases in which the leakage results from chronic inflammation or elevated VEGF and, in combination with VEGF, for promoting growth of nonleaky vessels.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Thurston, G -- Suri, C -- Smith, K -- McClain, J -- Sato, T N -- Yancopoulos, G D -- McDonald, D M -- HL-24136/HL/NHLBI NIH HHS/ -- HL-59157/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1999 Dec 24;286(5449):2511-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Anatomy and Cardiovascular Research Institute, University of California, San Francisco, CA 94143-0452, USA. gavint@itsa.ucsf.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10617467" target="_blank"〉PubMed〈/a〉
    Keywords: Angiopoietin-1 ; Animals ; Arterioles/anatomy & histology/physiology ; Binding Sites ; Capillaries/anatomy & histology/physiology ; *Capillary Permeability ; Ear ; Endothelial Growth Factors/genetics/*physiology ; Endothelium, Vascular/metabolism ; Inflammation/chemically induced ; Inflammation Mediators/pharmacology ; Lymphokines/genetics/*physiology ; Membrane Glycoproteins/genetics/*physiology ; Mice ; Mice, Transgenic ; Microcirculation/anatomy & histology/*physiology ; Mustard Plant ; *Neovascularization, Physiologic ; Plant Extracts/pharmacology ; Plant Lectins ; Plant Oils ; Plants, Medicinal ; Platelet Activating Factor/pharmacology ; Ricin/metabolism ; Serotonin/pharmacology ; Skin/blood supply/metabolism ; Vascular Endothelial Growth Factor A ; Vascular Endothelial Growth Factors ; Venules/anatomy & histology/physiology
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  • 18
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-01-15
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rasenick, M M -- New York, N.Y. -- Science. 1999 Dec 24;286(5449):2449-50.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10636805" target="_blank"〉PubMed〈/a〉
    Keywords: Commerce ; Cuba ; *International Cooperation ; *Neurosciences ; Politics ; Technology Transfer ; United States
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  • 19
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-01-15
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dwyer, D S -- New York, N.Y. -- Science. 1999 Dec 24;286(5449):2452.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10636807" target="_blank"〉PubMed〈/a〉
    Keywords: Financing, Government ; *National Institutes of Health (U.S.)/economics ; *Peer Review, Research ; *Research Support as Topic ; United States
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-09-30
    Description: Researchers have deciphered how a promising cancer drug acts like a smart bomb, homing in on only a very narrow range of its potential targets in the cell. The compound, known as STI-571, has shown remarkable success in early clinical trials on patients with chronic myelogenous leukemia. Now, in work reported on page 1938, scientists reveal just how the compound works--information that could aid in the design of similar cancer therapies.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Marx, J -- New York, N.Y. -- Science. 2000 Sep 15;289(5486):1857-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11012350" target="_blank"〉PubMed〈/a〉
    Keywords: Antineoplastic Agents/chemistry/*therapeutic use ; Benzamides ; Catalytic Domain/drug effects ; Clinical Trials as Topic ; Enzyme Activation/drug effects ; Enzyme Inhibitors/therapeutic use ; Humans ; Imatinib Mesylate ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*drug therapy/enzymology ; *Piperazines ; Protein Conformation ; Protein-Tyrosine Kinases/antagonists & inhibitors/chemistry ; Proto-Oncogene Proteins c-abl/*antagonists & inhibitors ; Pyrimidines/chemistry/*therapeutic use
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  • 21
    Publication Date: 2000-03-24
    Description: Selective microtubule orientation toward spatially defined cortical sites is critical to polarized cellular processes as diverse as axon outgrowth and T cell cytotoxicity. In yeast, oriented cytoplasmic microtubules align the mitotic spindle between mother and bud. The cortical marker protein Kar9 localizes to the bud tip and is required for the orientation of microtubules toward this region. Here, we show that Kar9 directs microtubule orientation by acting through Bim1, a conserved microtubule-binding protein. Bim1 homolog EB1 was originally identified through its interaction with adenomatous polyposis coli (APC) tumor suppressor, raising the possibility that an APC-EB1 linkage orients microtubules in higher cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Korinek, W S -- Copeland, M J -- Chaudhuri, A -- Chant, J -- GM07620-19/GM/NIGMS NIH HHS/ -- GM07620-20/GM/NIGMS NIH HHS/ -- GM49782/GM/NIGMS NIH HHS/ -- etc. -- New York, N.Y. -- Science. 2000 Mar 24;287(5461):2257-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10731146" target="_blank"〉PubMed〈/a〉
    Keywords: Adenomatous Polyposis Coli Protein ; Cell Cycle Proteins/genetics/*metabolism ; Cell Nucleus/physiology ; Cytoskeletal Proteins/metabolism ; Microtubule Proteins/genetics/*metabolism ; Microtubule-Associated Proteins/metabolism ; Microtubules/metabolism/*physiology ; Mutation ; Nuclear Proteins/genetics/*metabolism ; Phenotype ; Protein Binding ; Recombinant Fusion Proteins/metabolism ; Saccharomyces cerevisiae/cytology/genetics/*physiology ; *Saccharomyces cerevisiae Proteins ; Spindle Apparatus/*physiology ; Two-Hybrid System Techniques
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  • 22
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-09-23
    Description: The use of microarrays--slides or chips systematically dotted with DNA from thousands of genes--to determine gene expression patterns is providing a wealth of new information that should aid in cancer diagnosis and ultimately in therapy. In the past several months, researchers in several labs have used microarray technology to identify specific subtypes of a variety of cancers, including leukemias and lymphomas, the dangerous skin cancer melanoma, and breast cancer. In some cases, they can determine which cancers are likely to respond to current therapies and which aren't. In addition, the studies are giving researchers a fix on which genes are important for the development, maintenance, and spread of the various cancers, and are thus possible drug targets.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Marx, J -- New York, N.Y. -- Science. 2000 Sep 8;289(5485):1670-2.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11001727" target="_blank"〉PubMed〈/a〉
    Keywords: Disease Progression ; *Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Genes, Tumor Suppressor ; Humans ; Neoplasm Invasiveness ; Neoplasm Metastasis/genetics ; Neoplasms/*classification/diagnosis/*genetics/therapy ; *Oligonucleotide Array Sequence Analysis ; Oncogenes ; Prognosis
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-03-25
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pennisi, E -- New York, N.Y. -- Science. 2000 Mar 3;287(5458):1580.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10733420" target="_blank"〉PubMed〈/a〉
    Keywords: Archaea/growth & development/*isolation & purification/metabolism ; Geologic Sediments/*microbiology ; Hot Temperature ; Pacific Ocean
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-07-15
    Description: Mutation at the mouse progressive ankylosis (ank) locus causes a generalized, progressive form of arthritis accompanied by mineral deposition, formation of bony outgrowths, and joint destruction. Here, we show that the ank locus encodes a multipass transmembrane protein (ANK) that is expressed in joints and other tissues and controls pyrophosphate levels in cultured cells. A highly conserved gene is present in humans and other vertebrates. These results identify ANK-mediated control of pyrophosphate levels as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ho, A M -- Johnson, M D -- Kingsley, D M -- 5T32GM07365/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2000 Jul 14;289(5477):265-70.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Developmental Biology and Howard Hughes Medical Institute, Beckman Center B300, Stanford University School of Medicine, Stanford, CA 94305-5327, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10894769" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Arthritis/*genetics/metabolism/pathology ; Base Sequence ; Biological Transport ; COS Cells ; Calcinosis/*genetics ; Chromosome Mapping ; Cloning, Molecular ; Dna ; Diphosphates/*metabolism ; Durapatite/metabolism ; Gene Expression ; Genetic Complementation Test ; Humans ; Membrane Proteins/*genetics/metabolism/*physiology ; Mice ; Mice, Transgenic ; Molecular Sequence Data ; Mutation ; Phenotype ; Phosphate Transport Proteins ; Physical Chromosome Mapping ; Sequence Homology, Nucleic Acid ; Tissue Distribution
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-09-02
    Description: Although for the last 50 years, ethicists dealing with human experimentation have focused primarily on the need to protect individual research subjects and vulnerable groups, biomedical research, especially in genetics, now requires the establishment of standards for the protection of communities. We have developed such a strategy, based on five steps. (i) Identification of community characteristics relevant to the biomedical research setting, (ii) delineation of a typology of different types of communities using these characteristics, (iii) determination of the range of possible community protections, (iv) creation of connections between particular protections and one or more community characteristics necessary for its implementation, and (v) synthesis of community characteristics and possible protections to define protections appropriate for each type of community. Depending on the particular community, consent and consultation, consultation alone, or no added protections may be required for research.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Weijer, C -- Emanuel, E J -- New York, N.Y. -- Science. 2000 Aug 18;289(5482):1142-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Bioethics, Dalhousie University, Halifax, Nova Scotia, B3H 4H7, Canada. charles.weijer@dal.ca〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10970227" target="_blank"〉PubMed〈/a〉
    Keywords: *Bioethics ; *Culture ; Guidelines as Topic ; *Human Experimentation ; Humans ; Informed Consent ; Research/*standards ; *Residence Characteristics ; Truth Disclosure ; Vulnerable Populations
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-06-17
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Enserink, M -- New York, N.Y. -- Science. 2000 Jun 2;288(5471):1562-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10858127" target="_blank"〉PubMed〈/a〉
    Keywords: Budgets ; Developing Countries ; Drug Industry/economics ; Financing, Government ; Humans ; Immunization Programs ; National Institutes of Health (U.S.)/economics ; *Research Support as Topic ; United States ; *Vaccines
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-09-30
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sinclair, A R -- Ludwig, D -- Clark, C W -- New York, N.Y. -- Science. 2000 Sep 15;289(5486):1875.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11012354" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Conservation of Natural Resources/methods ; *Ecosystem ; Humans ; Residence Characteristics
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  • 28
    Publication Date: 2000-08-01
    Description: The path of the nucleic acids through a transcription elongation complex was tracked by mapping cross-links between bacterial RNA polymerase (RNAP) and transcript RNA or template DNA onto the x-ray crystal structure. In the resulting model, the downstream duplex DNA is nestled in a trough formed by the beta' subunit and enclosed on top by the beta subunit. In the RNAP channel, the RNA/DNA hybrid extends from the enzyme active site, along a region of the beta subunit harboring rifampicin resistance mutations, to the beta' subunit "rudder." The single-stranded RNA is then extruded through another channel formed by the beta-subunit flap domain. The model provides insight into the functional properties of the transcription complex.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Korzheva, N -- Mustaev, A -- Kozlov, M -- Malhotra, A -- Nikiforov, V -- Goldfarb, A -- Darst, S A -- GM30717/GM/NIGMS NIH HHS/ -- GM49242/GM/NIGMS NIH HHS/ -- GM53759/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2000 Jul 28;289(5479):619-25.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Public Health Research Institute, 455 First Avenue, New York, NY 10016, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10915625" target="_blank"〉PubMed〈/a〉
    Keywords: Binding Sites ; Cross-Linking Reagents ; Crystallography, X-Ray ; DNA/chemistry/genetics/*metabolism ; DNA Primers ; DNA-Directed RNA Polymerases/*chemistry/genetics/metabolism ; Models, Molecular ; Mutation ; Nucleic Acid Conformation ; Nucleic Acid Hybridization ; Oligodeoxyribonucleotides/chemistry/metabolism ; Oligoribonucleotides/chemistry/metabolism ; Protein Conformation ; Protein Structure, Tertiary ; RNA, Messenger/chemistry/genetics/*metabolism ; Templates, Genetic ; Thermus/enzymology ; *Transcription, Genetic
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-02-26
    Description: Stem cells are the natural units of embryonic generation, and also adult regeneration, of a variety of tissues. Recently, the list of tissues that use the model of differentiation from stem to progenitor to mature cell has increased from blood to include a variety of tissues, including both central and peripheral nervous systems and skeletal muscle; it is also possible that all organs and tissues are derived from, and still contain, stem cells. Because the number and activities of stem cells and their progeny are homeostatically regulated, clinical stem cell transplantation could greatly add to the physician's armamentarium against degenerative diseases.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Weissman, I L -- New York, N.Y. -- Science. 2000 Feb 25;287(5457):1442-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Departments of Pathology and Developmental Biology, Stanford University School of Medicine, Stanford, CA 94302-5323, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10688785" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Autoimmune Diseases/therapy ; Bone Marrow Transplantation ; Cell Differentiation ; Cell Division ; *Hematopoietic Stem Cell Transplantation ; Hematopoietic Stem Cells/cytology/*physiology ; Humans ; Immune Tolerance ; Neoplasms/therapy ; Nervous System Diseases/therapy ; Regeneration ; *Stem Cell Transplantation ; Stem Cells/cytology/*physiology ; Transplantation, Homologous
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-03-24
    Description: The 120-megabase euchromatic portion of the Drosophila melanogaster genome has been sequenced. Because the genome is compact and many genetic tools are available, and because fly cell biology and development have much in common with mammals, this sequence may be the Rosetta stone for deciphering the human genome.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kornberg, T B -- Krasnow, M A -- New York, N.Y. -- Science. 2000 Mar 24;287(5461):2218-20.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94143, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10731136" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Biology ; Cloning, Molecular ; DNA Transposable Elements ; Drosophila melanogaster/*genetics/physiology ; Genes, Insect ; *Genetics, Medical ; *Genome ; *Genome, Human ; Humans ; Mutation ; Physical Chromosome Mapping ; *Sequence Analysis, DNA
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-10-29
    Description: The central nervous system functions primarily to convert patterns of activity in sensory receptors into patterns of muscle activity that constitute appropriate behavior. At the anatomical level this requires two complementary processes: a set of genetically encoded rules for building the basic network of connections, and a mechanism for subsequently fine tuning these connections on the basis of experience. Identifying the locus and mechanism of these structural changes has long been among neurobiology's major objectives. Evidence has accumulated implicating a particular class of contacts, excitatory synapses made onto dendritic spines, as the sites where connective plasticity occurs. New developments in light microscopy allow changes in spine morphology to be directly visualized in living neurons and suggest that a common mechanism, based on dynamic actin filaments, is involved in both the formation of dendritic spines during development and their structural plasticity at mature synapses.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Matus, A -- New York, N.Y. -- Science. 2000 Oct 27;290(5492):754-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Friedrich Miescher Institute, Maulbeerstrasse 66, 4058 Basel, Switzerland. matus@fmi.ch〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11052932" target="_blank"〉PubMed〈/a〉
    Keywords: Actin Cytoskeleton/*physiology ; Actins/*physiology ; Animals ; Brain/embryology/growth & development/physiology ; Calcium/metabolism ; Dendrites/*physiology/ultrastructure ; Humans ; Learning ; Long-Term Potentiation ; Neural Pathways ; *Neuronal Plasticity ; Receptors, Glutamate/metabolism ; Synapses/*physiology
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-03-17
    Description: Driven by chemistry but increasingly guided by pharmacology and the clinical sciences, drug research has contributed more to the progress of medicine during the past century than any other scientific factor. The advent of molecular biology and, in particular, of genomic sciences is having a deep impact on drug discovery. Recombinant proteins and monoclonal antibodies have greatly enriched our therapeutic armamentarium. Genome sciences, combined with bioinformatic tools, allow us to dissect the genetic basis of multifactorial diseases and to determine the most suitable points of attack for future medicines, thereby increasing the number of treatment options. The dramatic increase in the complexity of drug research is enforcing changes in the institutional basis of this interdisciplinary endeavor. The biotech industry is establishing itself as the discovery arm of the pharmaceutical industry. In bridging the gap between academia and large pharmaceutical companies, the biotech firms have been effective instruments of technology transfer.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Drews, J -- New York, N.Y. -- Science. 2000 Mar 17;287(5460):1960-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉International Biomedicine Management Partners, Basel, Switzerland and Orbimed Advisors LLC, New York, NY 10017-2023, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10720314" target="_blank"〉PubMed〈/a〉
    Keywords: Chemistry, Pharmaceutical/*history ; Combinatorial Chemistry Techniques ; *Drug Design ; Drug Evaluation, Preclinical/history ; Drug Industry/*history ; History, 19th Century ; History, 20th Century ; Molecular Biology ; Pharmacology, Clinical ; Technology, Pharmaceutical/*history
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-04-15
    Description: The contribution of extrinsic and genetic mechanisms in determining areas of the mammalian neocortex has been a contested issue. This study analyzes the roles of the regulatory genes Emx2 and Pax6, which are expressed in opposing gradients in the neocortical ventricular zone, in specifying areas. Changes in the patterning of molecular markers and area-specific connections between the cortex and thalamus suggest that arealization of the neocortex is disproportionately altered in Emx2 and Pax6 mutant mice in opposing manners predicted from their countergradients of expression: rostral areas expand and caudal areas contract in Emx2 mutants, whereas the opposite effect is seen in Pax6 mutants. These findings suggest that Emx2 and Pax6 cooperate to regulate arealization of the neocortex and to confer area identity to cortical cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bishop, K M -- Goudreau, G -- O'Leary, D D -- NS31558/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 2000 Apr 14;288(5464):344-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10764649" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Brain Mapping ; Cadherins/biosynthesis/genetics ; DNA-Binding Proteins/*genetics/physiology ; Eye Proteins ; *Gene Expression ; Gene Expression Regulation, Developmental ; *Genes, Homeobox ; *Genes, Regulator ; Homeodomain Proteins/*genetics/physiology ; Mice ; Mice, Inbred C57BL ; Mice, Inbred DBA ; Mice, Mutant Strains ; Morphogenesis ; Neocortex/*embryology/metabolism ; Neural Pathways ; Occipital Lobe/embryology/metabolism ; Paired Box Transcription Factors ; Repressor Proteins ; Somatosensory Cortex/embryology/metabolism ; Thalamus/embryology ; Transcription Factors ; Visual Cortex/embryology/metabolism
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  • 34
    Publication Date: 2000-02-26
    Description: Most types of antibiotic resistance impose a biological cost on bacterial fitness. These costs can be compensated, usually without loss of resistance, by second-site mutations during the evolution of the resistant bacteria in an experimental host or in a laboratory medium. Different fitness-compensating mutations were selected depending on whether the bacteria evolved through serial passage in mice or in a laboratory medium. This difference in mutation spectra was caused by either a growth condition-specific formation or selection of the compensated mutants. These results suggest that bacterial evolution to reduce the costs of antibiotic resistance can take different trajectories within and outside a host.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bjorkman, J -- Nagaev, I -- Berg, O G -- Hughes, D -- Andersson, D I -- New York, N.Y. -- Science. 2000 Feb 25;287(5457):1479-82.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Bacteriology, Swedish Institute for Infectious Disease Control, S-171 82 Solna, Sweden.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10688795" target="_blank"〉PubMed〈/a〉
    Keywords: Adaptation, Physiological ; Animals ; Anti-Bacterial Agents/*pharmacology ; *Antiporters ; Carrier Proteins/genetics ; Culture Media ; Drug Resistance, Microbial/*genetics ; Escherichia coli Proteins ; Evolution, Molecular ; Female ; Fusidic Acid/pharmacology ; Membrane Proteins/genetics ; Mice ; Mice, Inbred BALB C ; *Mutation ; Peptide Elongation Factor G/genetics ; Ribosomal Proteins/genetics ; Salmonella typhimurium/*drug effects/*genetics/growth & development/metabolism ; Selection, Genetic ; Serial Passage ; Streptomycin/pharmacology ; Suppression, Genetic
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-02-26
    Description: Most, if not all, epithelial tissues contain stem cells. They are responsible for normal tissue renewal or for regeneration following damage. Our present knowledge of their properties is limited and is mainly derived from studies of cell kinetics and from clonal analysis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Slack, J M -- New York, N.Y. -- Science. 2000 Feb 25;287(5457):1431-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Developmental Biology Programme, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK. j.m.w.slack@bath.ac.uk.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10688782" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Cell Differentiation ; *Cell Division ; Clone Cells/cytology/physiology ; Embryo, Mammalian/cytology ; Epithelial Cells/*cytology/pathology/physiology ; Humans ; Metaplasia ; Regeneration ; Stem Cells/*cytology/physiology
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-02-12
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Smith, P -- Powlson, D S -- New York, N.Y. -- Science. 2000 Jan 21;287(5452):428-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10671170" target="_blank"〉PubMed〈/a〉
    Keywords: *Agriculture ; Animals ; *Animals, Domestic ; *Carbon ; *Manure ; Soil
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  • 37
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-05-20
    Description: Direct numerical simulations of wall-bounded flow reveal that turbulence production can be suppressed by a transverse traveling wave. Flow visualizations show that the near-wall streaks are eliminated, in contrast to other turbulence-control techniques, leading to a large shear stress reduction. The traveling wave can be induced by a spanwise force that is confined within the viscous sublayer; it has its maximum at the wall and decays exponentially away from it. We demonstrate the application of this approach in salt water, using arrays of electromagnetic tiles that produce the required traveling wave excitation at a high efficiency.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Du -- Karniadakis -- New York, N.Y. -- Science. 2000 May 19;288(5469):1230-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Division of Applied Mathematics, Brown University, Providence, RI 02912, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10817995" target="_blank"〉PubMed〈/a〉
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  • 38
    Publication Date: 2000-03-17
    Description: Allergic asthma is caused by the aberrant expansion in the lung of T helper cells that produce type 2 (TH2) cytokines and is characterized by infiltration of eosinophils and bronchial hyperreactivity. This disease is often triggered by mast cells activated by immunoglobulin E (IgE)-mediated allergic challenge. Activated mast cells release various chemical mediators, including prostaglandin D2 (PGD2), whose role in allergic asthma has now been investigated by the generation of mice deficient in the PGD receptor (DP). Sensitization and aerosol challenge of the homozygous mutant (DP-/-) mice with ovalbumin (OVA) induced increases in the serum concentration of IgE similar to those in wild-type mice subjected to this model of asthma. However, the concentrations of TH2 cytokines and the extent of lymphocyte accumulation in the lung of OVA-challenged DP-/- mice were greatly reduced compared with those in wild-type animals. Moreover, DP-/- mice showed only marginal infiltration of eosinophils and failed to develop airway hyperreactivity. Thus, PGD2 functions as a mast cell-derived mediator to trigger asthmatic responses.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Matsuoka, T -- Hirata, M -- Tanaka, H -- Takahashi, Y -- Murata, T -- Kabashima, K -- Sugimoto, Y -- Kobayashi, T -- Ushikubi, F -- Aze, Y -- Eguchi, N -- Urade, Y -- Yoshida, N -- Kimura, K -- Mizoguchi, A -- Honda, Y -- Nagai, H -- Narumiya, S -- New York, N.Y. -- Science. 2000 Mar 17;287(5460):2013-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto 606-8501, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10720327" target="_blank"〉PubMed〈/a〉
    Keywords: Allergens/immunology ; Animals ; Asthma/immunology/metabolism/pathology/*physiopathology ; Bronchial Hyperreactivity ; Bronchoalveolar Lavage Fluid/cytology/immunology ; Crosses, Genetic ; Female ; Gene Targeting ; Humans ; Immunoglobulin E/blood ; Interferon-gamma/metabolism ; Interleukins/metabolism ; Lung/immunology/metabolism/pathology ; Lymphocytes/immunology ; Male ; Mast Cells/metabolism ; Mice ; Mice, Inbred C57BL ; Mucus/secretion ; Ovalbumin/immunology ; Prostaglandin D2/metabolism/*physiology ; *Receptors, Immunologic ; Receptors, Prostaglandin/genetics/metabolism/*physiology ; Respiratory Mucosa/secretion
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  • 39
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-08-05
    Description: Reproductive cells that are destined to become sperm or egg undergo meiotic division during which the chromosome number is halved. As Sluder and McCollum explain in their Perspective, new findings (Shonn et al.) in yeast show that there is a spindle checkpoint that operates during meiosis to ensure that an equal number of replicated chromosomes arrives at each pole of the cell. One of the components of this meiotic spindle checkpoint turns out to be Mad2, which gives the signal to halt meiosis if it looks like unequal chromosome segregation is taking place.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sluder, G -- McCollum, D -- New York, N.Y. -- Science. 2000 Jul 14;289(5477):254-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA. greenfield.sluder@umassmed.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10917849" target="_blank"〉PubMed〈/a〉
    Keywords: Calcium-Binding Proteins/physiology ; *Carrier Proteins ; Cell Cycle Proteins ; Chromosome Aberrations ; Chromosome Disorders ; Chromosome Segregation ; Fungal Proteins/physiology ; Humans ; Meiosis/*physiology ; Nuclear Proteins ; Saccharomycetales/genetics/physiology ; Signal Transduction
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  • 40
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-03-25
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Enserink, M -- New York, N.Y. -- Science. 2000 Mar 3;287(5458):1571.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10733414" target="_blank"〉PubMed〈/a〉
    Keywords: *Anti-Infective Agents ; *Developing Countries ; *Drug Industry/economics ; European Union ; Financing, Government ; *Global Health ; Humans ; Immunization Programs ; Japan ; Technology, Pharmaceutical ; United States ; *Vaccines
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  • 41
    Publication Date: 2000-10-13
    Description: Signaling proteins are thought to be tightly regulated spatially and temporally in order to generate specific and localized effects. For Rac and other small guanosine triphosphatases, binding to guanosine triphosphate leads to interaction with downstream targets and regulates subcellular localization. A method called FLAIR (fluorescence activation indicator for Rho proteins) was developed to quantify the spatio-temporal dynamics of the Rac1 nucleotide state in living cells. FLAIR revealed precise spatial control of growth factor-induced Rac activation, in membrane ruffles and in a gradient of activation at the leading edge of motile cells. FLAIR exemplifies a generally applicable approach for examining spatio-temporal control of protein activity.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kraynov, V S -- Chamberlain, C -- Bokoch, G M -- Schwartz, M A -- Slabaugh, S -- Hahn, K M -- AG15430/AG/NIA NIH HHS/ -- GM39434/GM/NIGMS NIH HHS/ -- R01 GM-57464/GM/NIGMS NIH HHS/ -- etc. -- New York, N.Y. -- Science. 2000 Oct 13;290(5490):333-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11030651" target="_blank"〉PubMed〈/a〉
    Keywords: 3T3 Cells ; Actins/metabolism ; Animals ; Biosensing Techniques ; Blood ; Cell Membrane/*enzymology/physiology/ultrastructure ; *Cell Movement ; Cell Nucleus/*enzymology ; Cell Polarity ; Enzyme Activation ; Fluorescence ; Guanosine Triphosphate/*metabolism ; Mice ; Nuclear Envelope/enzymology ; Platelet-Derived Growth Factor/pharmacology ; Recombinant Fusion Proteins/metabolism ; Spectrometry, Fluorescence ; rac1 GTP-Binding Protein/*metabolism
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  • 42
    Publication Date: 2000-11-25
    Description: Biomolecular motors such as F1-adenosine triphosphate synthase (F1-ATPase) and myosin are similar in size, and they generate forces compatible with currently producible nanoengineered structures. We have engineered individual biomolecular motors and nanoscale inorganic systems, and we describe their integration in a hybrid nanomechanical device powered by a biomolecular motor. The device consisted of three components: an engineered substrate, an F1-ATPase biomolecular motor, and fabricated nanopropellers. Rotation of the nanopropeller was initiated with 2 mM adenosine triphosphate and inhibited by sodium azide.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Soong, R K -- Bachand, G D -- Neves, H P -- Olkhovets, A G -- Craighead, H G -- Montemagno, C D -- New York, N.Y. -- Science. 2000 Nov 24;290(5496):1555-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Nanobiotechnology Center, Department of Agricultural and Biological Engineering, School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11090349" target="_blank"〉PubMed〈/a〉
    Keywords: Adenosine Triphosphate/metabolism ; *Biotechnology/instrumentation/methods ; Biotinylation ; Enzyme Inhibitors ; *Miniaturization ; *Molecular Motor Proteins ; Nickel/metabolism ; *Proton-Translocating ATPases/antagonists & inhibitors/metabolism ; Recombinant Proteins ; Rotation ; Sodium Azide/pharmacology
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  • 43
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-04-01
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Brenner, S -- New York, N.Y. -- Science. 2000 Mar 24;287(5461):2173-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Molecular Sciences Institute, Berkeley, CA 94704, USA. brenner@molsci.org〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10744539" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Computational Biology ; Drosophila melanogaster/genetics ; *Gene Expression Regulation ; Genes ; *Genome ; *Molecular Biology ; *Proteins/chemistry/genetics/physiology ; Sequence Analysis, DNA
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  • 44
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-02-26
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Enserink, M -- New York, N.Y. -- Science. 2000 Feb 11;287(5455):951.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10691565" target="_blank"〉PubMed〈/a〉
    Keywords: *Biotechnology ; *Databases, Factual ; *Databases, Nucleic Acid ; *Genetic Research ; *Genetics, Medical ; Humans ; Iceland ; Medical Records Systems, Computerized
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  • 45
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-07-06
    Description: A human cytomegalovirus gene array was used to identify a previously unidentified class of viral transcripts. These transcripts, termed virion RNAs, were packaged within infectious virions and were delivered to the host cell on infection. This mechanism of herpesvirus gene expression allows for viral genes to be expressed within an infected cell immediately after virus entry and in the absence of transcription from the viral genome.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bresnahan, W A -- Shenk, T -- CA85786/CA/NCI NIH HHS/ -- F32 AI010448/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 2000 Jun 30;288(5475):2373-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10875924" target="_blank"〉PubMed〈/a〉
    Keywords: Cell Nucleus/metabolism ; Cells, Cultured ; Cytomegalovirus/*genetics/*physiology ; Dactinomycin/pharmacology ; Gene Expression ; Genes, Viral ; Genome, Viral ; Golgi Apparatus/metabolism ; Humans ; Nucleic Acid Hybridization ; Nucleic Acid Synthesis Inhibitors/pharmacology ; Oligonucleotide Array Sequence Analysis ; RNA, Messenger/*genetics/isolation & purification/metabolism ; RNA, Viral/*genetics/isolation & purification/metabolism ; Recombinant Fusion Proteins/metabolism ; Transcription, Genetic ; Viral Proteins/genetics/metabolism ; Virion/*genetics/physiology ; Virus Assembly
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  • 46
    Publication Date: 2000-06-10
    Description: Globally, tropical deforestation releases 20 to 30% of anthropogenic greenhouse gases. Conserving forests could reduce emissions, but the cost-effectiveness of this mechanism for mitigation depends on the associated opportunity costs. We estimated these costs from local, national, and global perspectives using a case study from Madagascar. Conservation generated significant benefits over logging and agriculture locally and globally. Nationally, however, financial benefits from industrial logging were larger than conservation benefits. Such differing economic signals across scales may exacerbate tropical deforestation. The Kyoto Protocol could potentially overcome this obstacle to conservation by creating markets for protection of tropical forests to mitigate climate change.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kremen, C -- Niles, J O -- Dalton, M G -- Daily, G C -- Ehrlich, P R -- Fay, J P -- Grewal, D -- Guillery, R P -- New York, N.Y. -- Science. 2000 Jun 9;288(5472):1828-32.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Conservation Biology, Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA. ckremen@stanford.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10846165" target="_blank"〉PubMed〈/a〉
    Keywords: Agriculture ; Carbon ; Conservation of Natural Resources/*economics ; Cost-Benefit Analysis ; Developed Countries ; Developing Countries ; *Ecosystem ; Greenhouse Effect ; Industry ; Madagascar ; *Trees
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  • 47
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-06-08
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Persaud, R -- New York, N.Y. -- Science. 2000 May 12;288(5468):975.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10841715" target="_blank"〉PubMed〈/a〉
    Keywords: Canada/epidemiology ; Depression/*epidemiology/etiology ; Depressive Disorder/*epidemiology/etiology ; Female ; Humans ; Male
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  • 48
    Publication Date: 2000-09-01
    Description: Activation of the transcription factor nuclear factor (NF)-kappaB by proinflammatory stimuli leads to increased expression of genes involved in inflammation. Activation of NF-kappaB requires the activity of an inhibitor of kappaB (IkappaB)-kinase (IKK) complex containing two kinases (IKKalpha and IKKbeta) and the regulatory protein NEMO (NF-kappaB essential modifier). An amino-terminal alpha-helical region of NEMO associated with a carboxyl-terminal segment of IKKalpha and IKKbeta that we term the NEMO-binding domain (NBD). A cell-permeable NBD peptide blocked association of NEMO with the IKK complex and inhibited cytokine-induced NF-kappaB activation and NF-kappaB-dependent gene expression. The peptide also ameliorated inflammatory responses in two experimental mouse models of acute inflammation. The NBD provides a target for the development of drugs that would block proinflammatory activation of the IKK complex without inhibiting basal NF-kappaB activity.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉May, M J -- D'Acquisto, F -- Madge, L A -- Glockner, J -- Pober, J S -- Ghosh, S -- AI 33443/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 2000 Sep 1;289(5484):1550-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Section of Immunobiology and Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10968790" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Animals ; Anti-Inflammatory Agents, Non-Steroidal/chemistry/pharmacology ; COS Cells ; Cells, Cultured ; E-Selectin/biosynthesis/genetics ; Endothelium, Vascular/metabolism ; Gene Expression Regulation ; HeLa Cells ; Humans ; I-kappa B Kinase ; Inflammation/drug therapy ; Mice ; Mice, Inbred C57BL ; Molecular Sequence Data ; Mutation ; NF-kappa B/*metabolism ; Peptides/chemistry/*pharmacology ; Point Mutation ; Protein Structure, Tertiary ; Protein-Serine-Threonine Kinases/chemistry/genetics/*metabolism ; Recombinant Fusion Proteins/metabolism
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  • 49
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-08-05
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Brownell, R L Jr -- Curry, B E -- Van Bonn, W -- Ridgway, S H -- New York, N.Y. -- Science. 2000 Jun 30;288(5475):2319-20.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10917829" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Animals, Wild ; California ; Communicable Diseases/transmission/*veterinary ; *Conservation of Natural Resources ; *Ecosystem ; *Seals, Earless
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  • 50
    Publication Date: 2000-08-29
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉White, S -- Meier, W -- Lovell, F -- McCoy, A -- Robinove, C J -- Creelan, T F -- Brun, R -- Gordon, I -- MacWest, R -- Collier, I E -- Gish, D T -- Hartmann, W K -- Behe, M J -- New York, N.Y. -- Science. 2000 Aug 11;289(5481):869-71.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10960317" target="_blank"〉PubMed〈/a〉
    Keywords: *Biological Evolution ; Biology/education ; Religion and Science ; Science/*education
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  • 51
    Publication Date: 2000-05-20
    Description: Galileo's photopolarimeter-radiometer instrument mapped Io's thermal emission during the I24, I25, and I27 flybys with a spatial resolution of 2.2 to 300 kilometers. Mapping of Loki in I24 shows uniform temperatures for most of Loki Patera and high temperatures in the southwest corner, probably resulting from an eruption that began 1 month before the observation. Most of Loki Patera was resurfaced before I27. Pele's caldera floor has a low temperature of 160 kelvin, whereas flows at Pillan and Zamama have temperatures of up to 200 kelvin. Global maps of nighttime temperatures provide a means for estimating global heat flow.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Spencer, J R -- Rathbun, J A -- Travis, L D -- Tamppari, L K -- Barnard, L -- Martin, T Z -- McEwen, A S -- New York, N.Y. -- Science. 2000 May 19;288(5469):1198-201.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Lowell Observatory, 1400 West Mars Hill Road, Flagstaff, AZ 86001, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10817987" target="_blank"〉PubMed〈/a〉
    Keywords: Darkness ; Extraterrestrial Environment ; Filtration ; *Hot Temperature ; *Jupiter ; Photometry ; *Space Flight ; Sunlight ; *Volcanic Eruptions
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  • 52
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-01-22
    Description: VDJ recombination is developmentally regulated in vivo by enhancer-dependent changes in the accessibility of chromosomal recombination signal sequences to the recombinase, but the molecular nature of these changes is unknown. Here histone H3 acetylation was measured along versions of a transgenic VDJ recombination reporter and the endogenous T cell receptor alpha/delta locus. Enhancer activity was shown to impart long-range, developmentally regulated changes in H3 acetylation, and H3 acetylation status was tightly linked to VDJ recombination. H3 hyperacetylation is proposed as a molecular mechanism coupling enhancer activity to accessibility for VDJ recombination.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉McMurry, M T -- Krangel, M S -- GM 41052/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2000 Jan 21;287(5452):495-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Immunology, Post Office Box 3010, Duke University Medical Center, Durham NC 27710, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10642553" target="_blank"〉PubMed〈/a〉
    Keywords: Acetylation ; Animals ; Chromatin/metabolism ; DNA Nucleotidyltransferases/metabolism ; DNA-Binding Proteins/metabolism ; Enhancer Elements, Genetic ; *Gene Rearrangement, T-Lymphocyte ; *Genes, T-Cell Receptor alpha ; Genes, T-Cell Receptor beta ; *Genes, T-Cell Receptor delta ; Histones/*metabolism ; Homeodomain Proteins/metabolism ; Humans ; Mice ; Mice, Transgenic ; Nuclear Proteins ; Protein Sorting Signals ; *Recombination, Genetic ; T-Lymphocytes/*metabolism ; Transgenes ; VDJ Recombinases
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  • 53
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-05-20
    Description: Spectroscopy of Io's Pele plume against Jupiter by the Hubble Space Telescope in October 1999 revealed absorption due to S2 gas, with a column density of 1.0 +/- 0.2 x 10(16) per square centimeter, and probably also SO(2) gas with a column density of 7 +/- 3 x 10(16) per square centimeter. This SO2/S2 ratio (3 to 12) is expected from equilibration with silicate magmas near the quartz-fayalite-magnetite or wustite-magnetite buffers. Condensed S3 and S4, probable coloring agents in Pele's red plume deposits, may form by polymerization of the S2, which is unstable to ultraviolet photolysis. Diffuse red deposits near other Io volcanoes suggest that venting and polymerization of S2 gas is a widespread feature of Io volcanism.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Spencer, J R -- Jessup, K L -- McGrath, M A -- Ballester, G E -- Yelle, R -- New York, N.Y. -- Science. 2000 May 19;288(5469):1208-10.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Lowell Observatory, 1400 West Mars Hill Road, Flagstaff, AZ 86001, USA. spencer@lowell.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10817990" target="_blank"〉PubMed〈/a〉
    Keywords: Extraterrestrial Environment ; *Gases ; *Jupiter ; Spacecraft ; Spectrum Analysis ; *Sulfur ; Sulfur Dioxide ; *Volcanic Eruptions
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  • 54
    Publication Date: 2000-03-04
    Description: The large chlorella virus PBCV-1, which contains double-stranded DNA (dsDNA), encodes a 94-codon open reading frame (ORF) that contains a motif resembling the signature sequence of the pore domain of potassium channel proteins. Phylogenetic analyses of the encoded protein, Kcv, indicate a previously unidentified type of potassium channel. The messenger RNA encoded by the ORF leads to functional expression of a potassium-selective conductance in Xenopus laevis oocytes. The channel blockers amantadine and barium, but not cesium, inhibit this conductance, in addition to virus plaque formation. Thus, PBCV-1 encodes the first known viral protein that functions as a potassium-selective channel and is essential in the virus life cycle.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Plugge, B -- Gazzarrini, S -- Nelson, M -- Cerana, R -- Van Etten, J L -- Derst, C -- DiFrancesco, D -- Moroni, A -- Thiel, G -- 971/Telethon/Italy -- GM32441/GM/NIGMS NIH HHS/ -- GM41333/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2000 Mar 3;287(5458):1641-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Albrecht-von-Haller-Institut fur Pflanzenwissenschaften, Universitat Gottingen, 37073 Gottingen, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10698737" target="_blank"〉PubMed〈/a〉
    Keywords: Amantadine/pharmacology ; Amino Acid Motifs ; Amino Acid Sequence ; Animals ; Barium/pharmacology ; Cesium/pharmacology ; Chlorella/virology ; Isoelectric Point ; Molecular Sequence Data ; Molecular Weight ; Oocytes ; Patch-Clamp Techniques ; Phycodnaviridae/chemistry/drug effects/*genetics/*physiology ; Potassium/metabolism ; Potassium Channels/*chemistry/genetics/*physiology ; RNA, Messenger/genetics/metabolism ; Recombinant Proteins/metabolism ; Sodium/metabolism ; Viral Plaque Assay ; *Viral Proteins ; Virus Replication/drug effects ; Xenopus laevis
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-06-17
    Description: Recent advances in observational and theoretical studies of El Nino have shed light on controversies concerning the possible effect of global warming on this phenomenon over the past few decades and in the future. El Nino is now understood to be one phase of a natural mode of oscillation-La Nina is the complementary phase-that results from unstable interactions between the tropical Pacific Ocean and the atmosphere. Random disturbances maintain this neutrally stable mode, whose properties depend on the background (time-averaged) climate state. Apparent changes in the properties of El Nino could reflect the importance of random disturbances, but they could also be a consequence of decadal variations of the background state. The possibility that global warming is affecting those variations cannot be excluded.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fedorov -- Philander -- New York, N.Y. -- Science. 2000 Jun 16;288(5473):1997-2002.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Geosciences, Princeton University, Princeton, NJ 08544, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10856205" target="_blank"〉PubMed〈/a〉
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  • 56
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-03-11
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Spengler, S J -- New York, N.Y. -- Science. 2000 Feb 18;287(5456):1221, 1223.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Bioinformatics and Computational Genomics, Berkeley, CA 94207, USA. sjspengler@lbl.gov〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10712158" target="_blank"〉PubMed〈/a〉
    Keywords: *Computational Biology/instrumentation/manpower/methods ; Computers ; Genome ; Intellectual Property ; Internet ; Oligonucleotide Array Sequence Analysis ; Proteins/chemistry/genetics/physiology ; Research Support as Topic ; Software
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  • 57
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-07-06
    Description: In this month's essay, Anne McLaren traces the winding and pitted pathways that connect the early days of the cell theory of biology in the 1830s to the new and unfolding era of cloning science and technology that came to worldwide attention in 1997 with the announcement of the birth of Dolly, the Scottish cloned sheep. The possibilities, including the potential for new medical treatments and perhaps even human cloning, are fantastic ... and ethically charged.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉McLaren, A -- New York, N.Y. -- Science. 2000 Jun 9;288(5472):1775-80.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Wellcome/CRC Institute, Cambridge, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10877698" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Animals, Domestic/embryology/genetics ; Bioethics ; Cell Differentiation ; Cell Line ; Cell Nucleus/physiology ; *Cloning, Organism/history/trends ; Cytoplasm/physiology ; Embryo, Mammalian/cytology ; Embryo, Nonmammalian/cytology ; History, 19th Century ; History, 20th Century ; Humans ; Nuclear Transfer Techniques ; Stem Cells/cytology/physiology ; Therapeutics
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  • 58
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-09-23
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Starck, W -- New York, N.Y. -- Science. 2000 Sep 8;289(5485):1691.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11001734" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Australia ; *Cnidaria ; Conservation of Natural Resources ; *Disasters ; *Ecosystem ; Humans
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  • 59
    Publication Date: 2000-03-24
    Description: We constructed a bacterial artificial chromosome (BAC)-based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hoskins, R A -- Nelson, C R -- Berman, B P -- Laverty, T R -- George, R A -- Ciesiolka, L -- Naeemuddin, M -- Arenson, A D -- Durbin, J -- David, R G -- Tabor, P E -- Bailey, M R -- DeShazo, D R -- Catanese, J -- Mammoser, A -- Osoegawa, K -- de Jong, P J -- Celniker, S E -- Gibbs, R A -- Rubin, G M -- Scherer, S E -- HG00750/HG/NHGRI NIH HHS/ -- New York, N.Y. -- Science. 2000 Mar 24;287(5461):2271-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Berkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. hoskins@bdgp.lbl.gov〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10731150" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Centromere/genetics ; Chromatin/genetics ; Chromosomes, Bacterial/genetics ; Cloning, Molecular ; *Contig Mapping ; DNA Fingerprinting ; Drosophila melanogaster/*genetics ; Euchromatin ; Gene Library ; Genes, Insect ; Genetic Markers ; Genetic Vectors ; *Genome ; In Situ Hybridization ; Repetitive Sequences, Nucleic Acid ; Restriction Mapping ; Sequence Analysis, DNA ; Sequence Tagged Sites ; Telomere/genetics
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  • 60
    Publication Date: 2000-03-31
    Description: The maternal determinant Bicoid (Bcd) represents the paradigm of a morphogen that provides positional information for pattern formation. However, as bicoid seems to be a recently acquired gene in flies, the question was raised as to how embryonic patterning is achieved in organisms with more ancestral modes of development. Because the phylogenetically conserved Hunchback (Hb) protein had previously been shown to act as a morphogen in abdominal patterning, we asked which functions of Bcd could be performed by Hb. By reestablishing a proposed ancient regulatory circuitry in which maternal Hb controls zygotic hunchback expression, we show that Hb is able to form thoracic segments in the absence of Bcd.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wimmer, E A -- Carleton, A -- Harjes, P -- Turner, T -- Desplan, C -- New York, N.Y. -- Science. 2000 Mar 31;287(5462):2476-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Lehrstuhl Genetik, Universitat Bayreuth, 95447 Bayreuth, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10741965" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Body Patterning ; DNA-Binding Proteins/genetics/*physiology ; Drosophila/*embryology/genetics ; *Drosophila Proteins ; Embryonic Development ; Female ; Gene Expression Regulation, Developmental ; Genes, Insect ; Homeodomain Proteins/genetics/*physiology ; Insect Proteins/genetics/*physiology ; Male ; Mutation ; Phenotype ; Promoter Regions, Genetic ; Thorax/embryology ; Trans-Activators/genetics/*physiology ; Transcription Factors/genetics/*physiology ; Transgenes ; Zinc Fingers ; Zygote/physiology
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  • 61
    Publication Date: 2000-10-20
    Description: A chemically induced dimer configuration was prepared on the silicon (Si) (100) surface and was characterized by scanning tunneling microscopy (STM) and spectroscopy (STS). These prepared dimers, which are essentially untilted and differ both electronically and structurally from the dynamically tilting dimers normally found on this surface, are more reactive than normal dimers. For molecular hydrogen (H2) adsorption, the enhancement is about 10(9) at room temperature. There is no appreciable barrier for the H2 reaction at prepared sites, indicating the prepared configuration closely approximates the actual dimer structure in the transition state. This previously unknown ability to prepare specific surface configurations has important implications for understanding and controlling reaction dynamics on semiconductor surfaces.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Buehler, E J -- Boland, J J -- New York, N.Y. -- Science. 2000 Oct 20;290(5491):506-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Venable and Kenan Laboratories, Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3290, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11039929" target="_blank"〉PubMed〈/a〉
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-07-06
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉McGrew, W C -- New York, N.Y. -- Science. 2000 Jun 9;288(5472):1747.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10877689" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Oral Hygiene ; *Pan troglodytes
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  • 63
    Publication Date: 2000-11-04
    Description: We report on the discovery of two emission features observed in the x-ray spectrum of the afterglow of the gamma-ray burst (GRB) of 16 December 1999 by the Chandra X-ray Observatory. These features are identified with the Ly(alpha) line and the narrow recombination continuum by hydrogenic ions of iron at a redshift z = 1.00 +/- 0.02, providing an unambiguous measurement of the distance of a GRB. Line width and intensity imply that the progenitor of the GRB was a massive star system that ejected, before the GRB event, a quantity of iron approximately 0.01 of the mass of the sun at a velocity approximately 0.1 of the speed of light, probably by a supernova explosion.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Piro, L -- Garmire, G -- Garcia, M -- Stratta, G -- Costa, E -- Feroci, M -- Meszaros, P -- Vietri, M -- Bradt, H -- Frail, D -- Frontera, F -- Halpern, J -- Heise, J -- Hurley, K -- Kawai, N -- Kippen, R M -- Marshall, F -- Murakami, T -- Sokolov, V V -- Takeshima, T -- Yoshida, A -- New York, N.Y. -- Science. 2000 Nov 3;290(5493):955-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Istituto Astrofisica Spaziale, Consiglio Nazionale delle Ricerche (CNR), Via Fosso del Cavaliere, 00133 Roma, Italy. piro@ias.rm.cnr.it〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11062121" target="_blank"〉PubMed〈/a〉
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-08-12
    Description: On page 441 of this issue, evolutionary biologists showcase the purple-throated carib hummingbird as a rare example of food supply--in this case, flower shape--spurring the evolution of a sexual dimorphism, or a feature that differs between males and females. On St. Lucia, an island in the West Indies, female caribs sport bills a third longer and twice as curved as those of their male counterparts--one of the most extreme bill differences between the sexes in any hummingbird species. In the paper, the researchers link these "whoppingly dimorphic bills" to the specific flowers the male and female caribs frequent.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Brown, K -- New York, N.Y. -- Science. 2000 Jul 21;289(5478):369-71.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10939937" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Beak/*anatomy & histology ; *Biological Evolution ; Birds/*anatomy & histology/physiology ; Ecosystem ; Feeding Behavior ; Female ; Male ; Plant Structures/anatomy & histology ; Saint Lucia ; *Sex Characteristics ; Zingiberales/*anatomy & histology
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-10-20
    Description: Using spontaneous parametric down-conversion, we produce polarization-entangled states of two photons and characterize them using two-photon tomography to measure the density matrix. A controllable decoherence is imposed on the states by passing the photons through thick, adjustable birefringent elements. When the system is subject to collective decoherence, one particular entangled state is seen to be decoherence-free, as predicted by theory. Such decoherence-free systems may have an important role for the future of quantum computation and information processing.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kwiat, P G -- Berglund, A J -- Altepeter, J B -- White, A G -- New York, N.Y. -- Science. 2000 Oct 20;290(5491):498-501.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Physics Division, P-23, Los Alamos National Laboratory, Los Alamos, NM 87545, USA. kwiat@lanl.gov〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11039926" target="_blank"〉PubMed〈/a〉
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-06-10
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Phillips, M -- New York, N.Y. -- Science. 2000 May 26;288(5470):1345; author reply 1346-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10847844" target="_blank"〉PubMed〈/a〉
    Keywords: *Bioethics ; Humans ; *Life Expectancy ; *Longevity
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  • 67
    Publication Date: 2000-02-26
    Description: The motion of individual cesium atoms trapped inside an optical resonator is revealed with the atom-cavity microscope (ACM). A single atom moving within the resonator generates large variations in the transmission of a weak probe laser, which are recorded in real time. An inversion algorithm then allows individual atom trajectories to be reconstructed from the record of cavity transmission and reveals single atoms bound in orbit by the mechanical forces associated with single photons. In these initial experiments, the ACM yields 2-micrometer spatial resolution in a 10-microsecond time interval. Over the duration of the observation, the sensitivity is near the standard quantum limit for sensing the motion of a cesium atom.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hood -- Lynn -- Doherty -- Parkins -- Kimble -- New York, N.Y. -- Science. 2000 Feb 25;287(5457):1447-53.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Norman Bridge Laboratory of Physics 12-33, California Institute of Technology, Pasadena, CA 91125, USA. Physics Department, The University of Auckland, Private Bag 92019, Auckland, New Zealand.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10688786" target="_blank"〉PubMed〈/a〉
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  • 68
    Publication Date: 2000-03-04
    Description: The peptidyl-prolyl isomerase Pin1 has been implicated in regulating cell cycle progression. Pin1 was found to be required for the DNA replication checkpoint in Xenopus laevis. Egg extracts depleted of Pin1 inappropriately transited from the G2 to the M phase of the cell cycle in the presence of the DNA replication inhibitor aphidicolin. This defect in replication checkpoint function was reversed after the addition of recombinant wild-type Pin1, but not an isomerase-inactive mutant, to the depleted extract. Premature mitotic entry in the absence of Pin1 was accompanied by hyperphosphorylation of Cdc25, activation of Cdc2/cyclin B, and generation of epitopes recognized by the mitotic phosphoprotein antibody, MPM-2. Therefore, Pin1 appears to be required for the checkpoint delaying the onset of mitosis in response to incomplete replication.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Winkler, K E -- Swenson, K I -- Kornbluth, S -- Means, A R -- CA 82845/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 2000 Mar 3;287(5458):1644-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology and Cancer Biology, Duke University Medical Center, Box 3813, Durham, NC 27710, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10698738" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Aphidicolin/pharmacology ; Cell Cycle ; *Cell Cycle Proteins ; Cyclin B/metabolism ; *DNA Replication ; Enzyme Inhibitors/pharmacology ; G2 Phase ; *Mitosis ; *Nuclear Proteins ; Nucleic Acid Synthesis Inhibitors ; Oocytes ; Peptidylprolyl Isomerase/genetics/*metabolism/pharmacology ; Point Mutation ; Protein Kinases/metabolism ; Protein-Serine-Threonine Kinases/metabolism ; Protein-Tyrosine Kinases/metabolism ; Recombinant Proteins/metabolism/pharmacology ; *Xenopus Proteins ; Xenopus laevis ; cdc25 Phosphatases/metabolism
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-03-11
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Winn, O H -- New York, N.Y. -- Science. 2000 Feb 18;287(5456):1204.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10712152" target="_blank"〉PubMed〈/a〉
    Keywords: Humans ; *Science
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  • 70
    Publication Date: 2000-12-16
    Description: Class I major histocompatibility complex (class I MHC) molecules, known to be important for immune responses to antigen, are expressed also by neurons that undergo activity-dependent, long-term structural and synaptic modifications. Here, we show that in mice genetically deficient for cell surface class I MHC or for a class I MHC receptor component, CD3zeta, refinement of connections between retina and central targets during development is incomplete. In the hippocampus of adult mutants, N-methyl-D-aspartate receptor-dependent long-term potentiation (LTP) is enhanced, and long-term depression (LTD) is absent. Specific class I MHC messenger RNAs are expressed by distinct mosaics of neurons, reflecting a potential for diverse neuronal functions. These results demonstrate an important role for these molecules in the activity-dependent remodeling and plasticity of connections in the developing and mature mammalian central nervous system (CNS).〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175035/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175035/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Huh, G S -- Boulanger, L M -- Du, H -- Riquelme, P A -- Brotz, T M -- Shatz, C J -- 1F32EY07016/EY/NEI NIH HHS/ -- EY06912/EY/NEI NIH HHS/ -- F32 EY007016/EY/NEI NIH HHS/ -- F32 EY007016-02/EY/NEI NIH HHS/ -- F32 EY007016-03/EY/NEI NIH HHS/ -- MH48108/MH/NIMH NIH HHS/ -- New York, N.Y. -- Science. 2000 Dec 15;290(5499):2155-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Neurobiology, Harvard Medical School, 220 Longwood Avenue, Boston, MA 02115, USA. gshuh@alum.mit.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11118151" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Antigens, CD3/genetics/*physiology ; Brain/growth & development/*physiology ; Excitatory Postsynaptic Potentials ; Gene Expression Profiling ; Genes, MHC Class I ; Geniculate Bodies/physiology ; Hippocampus/growth & development/physiology ; Histocompatibility Antigens Class I/genetics/*physiology ; In Situ Hybridization ; Long-Term Potentiation ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Mice, Mutant Strains ; Neural Pathways ; *Neuronal Plasticity ; Neurons/*physiology ; Receptors, GABA-A/metabolism ; Receptors, N-Methyl-D-Aspartate/metabolism ; Retina/growth & development/physiology ; Retinal Ganglion Cells/physiology ; Signal Transduction ; Synapses/*physiology ; Synaptic Transmission ; Visual Pathways
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-08-11
    Description: Regional-scale undulations with associated small-scale secondary structures are inferred to be folds on Jupiter's moon Europa. Formation is consistent with stresses from tidal deformation, potentially triggering compressional instability of a region of locally high thermal gradient. Folds may compensate for extension elsewhere on Europa and then relax away over time.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Prockter, L M -- Pappalardo, R T -- New York, N.Y. -- Science. 2000 Aug 11;289(5481):941-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Applied Physics Laboratory, Johns Hopkins University, MS 7-366, 11100 Johns Hopkins Road, Laurel, MD 20723, USA. louise.prockter@jhuapl.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10937995" target="_blank"〉PubMed〈/a〉
    Keywords: Extraterrestrial Environment ; Ice ; *Jupiter ; Temperature
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-05-08
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Marshall, E -- New York, N.Y. -- Science. 2000 Apr 28;288(5466):587.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10798985" target="_blank"〉PubMed〈/a〉
    Keywords: National Institutes of Health (U.S.)/*organization & administration ; Politics ; United States
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  • 73
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-09-29
    Description: Pore waters associated with gas hydrates at Blake Ridge in the Atlantic Ocean were dated by measuring their iodine-129/iodine ratios. Samples collected from sediments with ages between 1.8 and 6 million years ago consistently yield ages around 55 million years ago. These ages, together with the strong iodine enrichment observed in the pore waters, suggest that the origin of iodine is related to organic material of early Tertiary age, which probably is also the source of the methane in the gas hydrates at this location.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fehn -- Snyder -- Egeberg -- New York, N.Y. -- Science. 2000 Sep 29;289(5488):2332-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Earth and Environmental Sciences, University of Rochester, Rochester, NY 14627, USA. Department of Chemistry, Agder College, Tordenskjoldgate 65, 4604 Kristiansand, Norway.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11009415" target="_blank"〉PubMed〈/a〉
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  • 74
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-04-25
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Marshall, E -- New York, N.Y. -- Science. 2000 Apr 14;288(5464):255-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10777404" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Biotechnology ; Drug Industry ; Europe ; Genome ; Mice ; *Mice, Knockout/genetics ; *Mice, Transgenic/genetics ; National Institutes of Health (U.S.) ; *Patents as Topic ; *Research/legislation & jurisprudence ; Sequence Analysis, DNA ; Technology Transfer ; United States ; Universities
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  • 75
    Publication Date: 2000-06-24
    Description: We describe the design and synthesis of a ferroelectric liquid crystal composed of racemic molecules. The ferroelectric polarization results from spontaneous polar symmetry breaking in a fluid smectic. The ferroelectric phase is also chiral, resulting in the formation of a mixture of macroscopic domains of either handedness at the isotropic-to-liquid crystal phase transition. This smectic liquid crystal is thus a fluid conglomerate. Detailed investigation of the electrooptic and polarization current behavior within individual domains in liquid crystal cells shows the thermodynamically stable structure to be a uniformly tilted smectic bow-phase (banana phase), with all layer pairs homochiral and ferroelectric (SmC(S)P(F)).〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Walba -- Korblova -- Shao -- Maclennan -- Link -- Glaser -- Clark -- New York, N.Y. -- Science. 2000 Jun 23;288(5474):2181-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry and Biochemistry and Ferroelectric Liquid Crystal Materials Research Center, University of Colorado, Boulder, CO 80309-0215, USA. Department of Physics and Ferroelectric Liquid Crystal Materials Research Center, University.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10864861" target="_blank"〉PubMed〈/a〉
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  • 76
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-08-12
    Description: On page 628, neuroscientists report that painful stimuli delivered to rats shortly after birth permanently rewire the spinal cord circuits that respond to pain. Not only do the circuits contain more axons, but the axons extend to more areas of the spinal cord than they normally would. The results should help convince skeptics of the importance of managing pain in human infants.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Helmuth, L -- New York, N.Y. -- Science. 2000 Jul 28;289(5479):521-2.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10939955" target="_blank"〉PubMed〈/a〉
    Keywords: Afferent Pathways ; Aging ; Animals ; Animals, Newborn ; Axons/*physiology ; Hindlimb/innervation ; Humans ; Infant, Newborn ; Inflammation/physiopathology ; *Pain ; Pain Threshold ; Rats ; Sciatic Nerve/*anatomy & histology/physiology ; Spinal Cord/*cytology
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-06-08
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bloom, F -- New York, N.Y. -- Science. 2000 May 12;288(5468):973.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10841713" target="_blank"〉PubMed〈/a〉
    Keywords: *Genome, Plant ; *Intellectual Property ; International Cooperation ; Oryza/*genetics ; Patents as Topic ; Private Sector ; Public Sector ; Sequence Analysis, DNA ; *Technology Transfer
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  • 78
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-08-12
    Description: Experts in the young field of violence epidemiology blame guns and crack cocaine for America's deadly crime surge in the early 1990s. Explaining the subsequent decline in violent crime rates has been more difficult, however. Some of the factors that seem to have helped squelch crime could be temporary, such as low unemployment rates. But others, including a growing intolerance for violence as a means of settling interpersonal disputes, seem to have become cultural norms.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Helmuth, L -- New York, N.Y. -- Science. 2000 Jul 28;289(5479):582-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10939973" target="_blank"〉PubMed〈/a〉
    Keywords: Abortion, Legal ; Crack Cocaine ; Domestic Violence/statistics & numerical data ; Female ; Firearms ; Homicide/*statistics & numerical data ; Humans ; Male ; Police ; Prisons ; Street Drugs ; United States ; Violence/*statistics & numerical data
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  • 79
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-04-15
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Buc, H -- New York, N.Y. -- Science. 2000 Mar 31;287(5462):2437-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institut Pasteur, Unit de Physicochimie des Macromolcules Biologiques (CNRS: URA 1773), 75724 Paris Cedex 15, France. henribuc@pasteur.fr〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10766622" target="_blank"〉PubMed〈/a〉
    Keywords: DNA/metabolism ; DNA-Directed RNA Polymerases/*metabolism ; RNA, Messenger/biosynthesis/metabolism ; *Terminator Regions, Genetic ; Transcription Factors/metabolism ; *Transcription, Genetic
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  • 80
    Publication Date: 2000-03-04
    Description: To determine the ability of antibodies to provide protection from Ebola viruses, monoclonal antibodies (mAbs) to the Ebola glycoprotein were generated and evaluated for efficacy. We identified several protective mAbs directed toward five unique epitopes on Ebola glycoprotein. One of the epitopes is conserved among all Ebola viruses that are known to be pathogenic for humans. Some protective mAbs were also effective therapeutically when administered to mice 2 days after exposure to lethal Ebola virus. The identification of protective mAbs has important implications for developing vaccines and therapies for Ebola virus.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wilson, J A -- Hevey, M -- Bakken, R -- Guest, S -- Bray, M -- Schmaljohn, A L -- Hart, M K -- New York, N.Y. -- Science. 2000 Mar 3;287(5458):1664-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Virology Division, U.S. Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, Frederick, MD 21702-5011, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10698744" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Antibodies, Monoclonal/*immunology ; Antibodies, Viral/*immunology ; Antibody Affinity ; Antigens, Viral/immunology ; Binding, Competitive ; Complement System Proteins/immunology ; Ebolavirus/*immunology/physiology ; Epitopes/immunology ; Female ; Hemorrhagic Fever, Ebola/*prevention & control/therapy ; Humans ; Immunoglobulin G/immunology ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Neutralization Tests ; Specific Pathogen-Free Organisms ; Viral Envelope Proteins/*immunology ; Viral Plaque Assay
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  • 81
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-09-09
    Description: Researchers are uncovering disturbing evidence that scientists and tourists are infecting wild primates with human pathogens. In response, ape specialists, including the American Society of Primatologists, are now calling for stricter health standards for researchers and tourists. They are also urging researchers to learn how to diagnose disease in their study animals.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ferber, D -- New York, N.Y. -- Science. 2000 Aug 25;289(5483):1277-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10979848" target="_blank"〉PubMed〈/a〉
    Keywords: Africa/epidemiology ; Animals ; Ape Diseases/epidemiology/*transmission ; Communicable Diseases/epidemiology/transmission/*veterinary ; *Gorilla gorilla ; Humans ; Parasitic Diseases, Animal/epidemiology/*transmission ; Primate Diseases/epidemiology/*transmission ; Primates ; Research Personnel ; Travel
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  • 82
    Publication Date: 2000-05-20
    Description: During late 1999/early 2000, the solid state imaging experiment on the Galileo spacecraft returned more than 100 high-resolution (5 to 500 meters per pixel) images of volcanically active Io. We observed an active lava lake, an active curtain of lava, active lava flows, calderas, mountains, plateaus, and plains. Several of the sulfur dioxide-rich plumes are erupting from distal flows, rather than from the source of silicate lava (caldera or fissure, often with red pyroclastic deposits). Most of the active flows in equatorial regions are being emplaced slowly beneath insulated crust, but rapidly emplaced channelized flows are also found at all latitudes. There is no evidence for high-viscosity lava, but some bright flows may consist of sulfur rather than mafic silicates. The mountains, plateaus, and calderas are strongly influenced by tectonics and gravitational collapse. Sapping channels and scarps suggest that many portions of the upper approximately 1 kilometer are rich in volatiles.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉McEwen, A S -- Belton, M J -- Breneman, H H -- Fagents, S A -- Geissler, P -- Greeley, R -- Head, J W -- Hoppa, G -- Jaeger, W L -- Johnson, T V -- Keszthelyi, L -- Klaasen, K P -- Lopes-Gautier, R -- Magee, K P -- Milazzo, M P -- Moore, J M -- Pappalardo, R T -- Phillips, C B -- Radebaugh, J -- Schubert, G -- Schuster, P -- Simonelli, D P -- Sullivan, R -- Thomas, P C -- Turtle, E P -- Williams, D A -- New York, N.Y. -- Science. 2000 May 19;288(5469):1193-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Lunar and Planetary Laboratory, University of Arizona, Tucson, AZ 85721, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10817986" target="_blank"〉PubMed〈/a〉
    Keywords: *Extraterrestrial Environment ; Geological Phenomena ; Geology ; Image Enhancement ; *Jupiter ; *Space Flight ; Spectrophotometry, Infrared ; *Volcanic Eruptions
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  • 83
    Publication Date: 2000-01-15
    Description: Murine T10 and T22 are highly related nonclassical major histocompatibility complex (MHC) class Ib proteins that bind to certain gammadelta T cell receptors (TCRs) in the absence of other components. The crystal structure of T22b at 3.1 angstroms reveals similarities to MHC class I molecules, but one side of the normal peptide-binding groove is severely truncated, which allows direct access to the beta-sheet floor. Potential gammadelta TCR-binding sites can be inferred from functional mapping of T10 and T22 point mutants and allelic variants. Thus, T22 represents an unusual variant of the MHC-like fold and indicates that gammadelta and alphabeta TCRs interact differently with their respective MHC ligands.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wingren, C -- Crowley, M P -- Degano, M -- Chien, Y -- Wilson, I A -- AI33431/AI/NIAID NIH HHS/ -- CA58896/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 2000 Jan 14;287(5451):310-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10634787" target="_blank"〉PubMed〈/a〉
    Keywords: Alleles ; Amino Acid Substitution ; Animals ; Binding Sites ; Crystallography, X-Ray ; Glycosylation ; Histocompatibility Antigens Class I/*chemistry ; Hydrogen Bonding ; Ligands ; Mice ; Models, Molecular ; Point Mutation ; Protein Conformation ; Protein Folding ; Protein Structure, Quaternary ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Proteins/*chemistry/immunology/metabolism ; Receptors, Antigen, T-Cell, gamma-delta/immunology/*metabolism ; Surface Properties ; beta 2-Microglobulin/chemistry
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  • 84
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-08-12
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sodha, N -- Williams, R -- Mangion, J -- Bullock, S L -- Yuille, M R -- Eeles, R A -- New York, N.Y. -- Science. 2000 Jul 21;289(5478):359.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Royal Marsden NHS Trust, Sutton, Surrey SM2 5PT, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10939935" target="_blank"〉PubMed〈/a〉
    Keywords: Checkpoint Kinase 2 ; Chromosomes, Human, Pair 15/genetics ; DNA Mutational Analysis ; Exons ; Gene Duplication ; Genetic Variation ; Humans ; Li-Fraumeni Syndrome/*genetics ; Mutation ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Protein Kinases/*genetics ; *Protein-Serine-Threonine Kinases ; Sequence Homology, Nucleic Acid
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  • 85
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-01-15
    Description: The memory consolidation hypothesis proposed 100 years ago by Muller and Pilzecker continues to guide memory research. The hypothesis that new memories consolidate slowly over time has stimulated studies revealing the hormonal and neural influences regulating memory consolidation, as well as molecular and cellular mechanisms. This review examines the progress made over the century in understanding the time-dependent processes that create our lasting memories.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉McGaugh, J L -- MH12526/MH/NIMH NIH HHS/ -- New York, N.Y. -- Science. 2000 Jan 14;287(5451):248-51.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉the Center for the Neurobiology of Learning and Memory and the Department of Neurobiology and Behavior, University of California Irvine, Irvine, CA 92697, USA. jlmcgaug@uci.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10634773" target="_blank"〉PubMed〈/a〉
    Keywords: Amnesia, Retrograde/physiopathology ; Amygdala/*physiology ; Animals ; Brain/*physiology ; Emotions ; Hippocampus/physiology ; Humans ; Long-Term Potentiation ; Memory/*physiology ; Memory, Short-Term/physiology ; Protein Kinases/metabolism ; Receptors, Adrenergic, beta/physiology
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  • 86
    Publication Date: 2000-08-26
    Description: The cytokine interleukin-10 (IL-10) has shown promise in clinical trials for treatment of inflammatory bowel disease (IBD). Using two mouse models, we show that the therapeutic dose of IL-10 can be reduced by localized delivery of a bacterium genetically engineered to secrete the cytokine. Intragastric administration of IL-10-secreting Lactococcus lactis caused a 50% reduction in colitis in mice treated with dextran sulfate sodium and prevented the onset of colitis in IL-10(-/-) mice. This approach may lead to better methods for cost-effective and long-term management of IBD in humans.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Steidler, L -- Hans, W -- Schotte, L -- Neirynck, S -- Obermeier, F -- Falk, W -- Fiers, W -- Remaut, E -- New York, N.Y. -- Science. 2000 Aug 25;289(5483):1352-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology, Ghent University and Flanders Interuniversity Institute for Biotechnology, K. L. Ledeganckstraat 35, 9000 Gent, Belgium. lothar.steidler@dmb.rug.ac.be〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10958782" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Biological Transport ; Colitis/immunology/pathology/prevention & control/therapy ; Colon/immunology/metabolism/microbiology/pathology ; Dextran Sulfate ; Inflammatory Bowel Diseases/immunology/pathology/prevention & control/*therapy ; Interleukin-10/*administration & dosage/*biosynthesis/genetics/metabolism ; Intestinal Mucosa/metabolism/pathology ; Lactococcus lactis/*genetics/immunology/*metabolism ; Mice ; Probiotics/*therapeutic use ; Recombinant Proteins/administration & dosage/biosynthesis/metabolism
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  • 87
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-03-11
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Brown, K S -- New York, N.Y. -- Science. 2000 Feb 18;287(5456):1192-3, 1195.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10712147" target="_blank"〉PubMed〈/a〉
    Keywords: *Biology ; *Conservation of Natural Resources ; Ecology ; Ecosystem ; Politics ; *Public Policy ; United States
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  • 88
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-06-10
    Description: The development of an eight-pen nanoplotter capable of doing parallel dip-pen nanolithography (DPN) is reported. Because line width and patterning speed in DPN are independent of contact force, only one of the tips in the parallel writing mode (the "imaging" tip) has a feedback system to monitor tip position and to write the pattern; all other tips reproduce what occurs at the imaging tip in a passive fashion. Proof-of-concept experiments that demonstrate eight-pen parallel writing, ink and rinsing wells, and "molecular corralling" via a nanoplotter-generated structure are reported.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hong -- Mirkin -- New York, N.Y. -- Science. 2000 Jun 9;288(5472):1808-11.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry and Northwestern University Center for Nanofabrication and Molecular Self-Assembly, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10846159" target="_blank"〉PubMed〈/a〉
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  • 89
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-04-01
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Marshall, E -- New York, N.Y. -- Science. 2000 Mar 24;287(5461):2127.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10744525" target="_blank"〉PubMed〈/a〉
    Keywords: Biotechnology/*economics ; Computational Biology ; Databases, Factual ; Genetics, Medical/economics ; *Genome, Human ; Great Britain ; Humans ; International Cooperation ; *Investments ; Molecular Biology/*economics ; Patents as Topic ; Public Policy ; *Sequence Analysis, DNA ; United States
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  • 90
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-09-09
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pennisi, E -- New York, N.Y. -- Science. 2000 Aug 25;289(5483):1267-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10979842" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Financing, Government ; *Genome ; Human Genome Project ; Humans ; Mice/genetics ; National Institutes of Health (U.S.)/economics ; Rats/*genetics ; Research Support as Topic ; *Sequence Analysis, DNA ; United States
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 91
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-07-15
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Campbell, P N -- New York, N.Y. -- Science. 2000 Jun 23;288(5474):2131.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10896581" target="_blank"〉PubMed〈/a〉
    Keywords: Armenia ; *Delivery of Health Care ; Humans ; Moldova
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  • 92
    Publication Date: 2000-07-07
    Description: A scanning probe technique was used to obtain a high-resolution map of the random electrostatic potential inside the quantum Hall liquid. A sharp metal tip, scanned above a semiconductor surface, sensed charges in an embedded two-dimensional (2D) electron gas. Under quantum Hall effect conditions, applying a positive voltage to the tip locally enhanced the 2D electron density and created a "bubble" of electrons in an otherwise unoccupied Landau level. As the tip scanned along the sample surface, the bubble followed underneath. The tip sensed the motions of single electrons entering or leaving the bubble in response to changes in the local 2D electrostatic potential.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Finkelstein -- Glicofridis -- Ashoori -- Shayegan -- New York, N.Y. -- Science. 2000 Jul 7;289(5476):90-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physics and Center for Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Department of Electrical Engineering, Princeton University, Princeton, NJ 08544, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10884231" target="_blank"〉PubMed〈/a〉
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  • 93
    Publication Date: 2000-06-17
    Description: We describe a distinct type of spontaneous hierarchical self-assembly of cytoskeletal filamentous actin (F-actin), a highly charged polyelectrolyte, and cationic lipid membranes. On the mesoscopic length scale, confocal microscopy reveals ribbonlike tubule structures that connect to form a network of tubules on the macroscopic scale (more than 100 micrometers). Within the tubules, on the 0.5- to 50-nanometer length scale, x-ray diffraction reveals an unusual structure consisting of osmotically swollen stacks of composite membranes with no direct analog in simple amphiphilic systems. The composite membrane is composed of three layers, a lipid bilayer sandwiched between two layers of actin, and is reminiscent of multilayered bacterial cell walls that exist far from equilibrium. Electron microscopy reveals that the actin layer consists of laterally locked F-actin filaments forming an anisotropic two-dimensional tethered crystal that appears to be the origin of the tubule formation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wong, G C -- Tang, J X -- Lin, A -- Li, Y -- Janmey, P A -- Safinya, C R -- AR38910/AR/NIAMS NIH HHS/ -- GM59288/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2000 Jun 16;288(5473):2035-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Materials Department, University of California, Santa Barbara, CA 93106, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10856215" target="_blank"〉PubMed〈/a〉
    Keywords: Actins/*chemistry/ultrastructure ; Anisotropy ; Cations ; Crystallization ; Electrochemistry ; Electrolytes ; Fatty Acids, Monounsaturated/chemistry ; Freeze Fracturing ; Lipid Bilayers/*chemistry ; Microscopy, Confocal ; Microscopy, Electron ; Molecular Conformation ; Protein Conformation ; Quaternary Ammonium Compounds/chemistry ; X-Ray Diffraction
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  • 94
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-10-14
    Description: Model organisms such as yeast have proved exceptionally valuable for revealing new information about the molecular pathways involved in the aging of cells. In her Perspective, Campisi comments on new work showing that caloric restriction increases longevity in yeast by activating the SIR2 gene, which alters the compactness of chromatin and thus regulates gene expression (Lin et al.).〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Campisi, J -- New York, N.Y. -- Science. 2000 Sep 22;289(5487):2062-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. jcampisi@lbl.gov〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11032557" target="_blank"〉PubMed〈/a〉
    Keywords: Aging/*physiology ; Animals ; Cell Division ; Chromatin/*physiology ; DNA Repair ; DNA Replication ; DNA, Circular/metabolism ; DNA, Fungal/metabolism ; DNA, Ribosomal/metabolism ; *Energy Intake ; *Gene Silencing ; Glucose/metabolism ; Histone Deacetylases/genetics/*metabolism ; Histones/metabolism ; Longevity ; Mutation ; NAD/metabolism ; Reactive Oxygen Species/metabolism ; Recombination, Genetic ; Saccharomyces cerevisiae/genetics/*physiology ; *Silent Information Regulator Proteins, Saccharomyces cerevisiae ; Sirtuin 2 ; Sirtuins ; Trans-Activators/genetics/*metabolism
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 95
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-06-08
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Campbell, S -- New York, N.Y. -- Science. 2000 May 19;288(5469):1174-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10841737" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Biological Clocks/*physiology ; Body Temperature ; Circadian Rhythm/*physiology ; Darkness ; Humans
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 96
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-09-30
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mervis, J -- New York, N.Y. -- Science. 2000 Sep 15;289(5486):1854-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11012348" target="_blank"〉PubMed〈/a〉
    Keywords: Education, Graduate ; Job Satisfaction ; National Institutes of Health (U.S.) ; *Research ; *Research Personnel/economics/statistics & numerical data ; Salaries and Fringe Benefits ; United States ; Workload
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  • 97
    Publication Date: 2000-04-25
    Description: Computerized tomography scans of a ferruginous concretion within the chest region of an ornithischian dinosaur reveal structures that are suggestive of a four-chambered heart and a single systemic aorta. The apparently derived condition of the cardiovascular system in turn suggests the existence of intermediate-to-high metabolic rates among dinosaurs.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fisher, P E -- Russell, D A -- Stoskopf, M K -- Barrick, R E -- Hammer, M -- Kuzmitz, A A -- New York, N.Y. -- Science. 2000 Apr 21;288(5465):503-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Biomedical Imaging Facility, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA. PaulvFisher@ncsu.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10775107" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Aorta/anatomy & histology ; Basal Metabolism ; Birds/anatomy & histology/metabolism ; Body Weight ; *Fossils ; Heart/*anatomy & histology/radiography ; Image Processing, Computer-Assisted ; Iron Compounds/analysis ; Minerals ; Paleontology ; Reptiles/*anatomy & histology/*metabolism ; Tomography Scanners, X-Ray Computed ; Tomography, X-Ray Computed ; X-Ray Diffraction
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 98
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-09-02
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Stokstad, E -- New York, N.Y. -- Science. 2000 Aug 18;289(5482):1122.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10970219" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Conservation of Natural Resources ; *Horseshoe Crabs ; Virginia
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  • 99
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-07-15
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mervis, J -- New York, N.Y. -- Science. 2000 Jun 23;288(5474):2115.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10896575" target="_blank"〉PubMed〈/a〉
    Keywords: Budgets ; *Financing, Government ; Government ; Maryland ; *Research Support as Topic ; United States
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 100
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2000-08-05
    Description: Caveolae are subcellular structures implicated in the import and transcytosis of macromolecules and in transmembrane signaling. To date, evidence for the existence of caveolae in hematopoietic cells has been ambiguous. Caveolae were detected in the microvilli and intracellular vesicles of cultured mouse bone marrow-derived mast cells (BMMCs). CD48, a receptor for FimH-expressing (type 1 fimbriated) Escherichia coli, was specifically localized to plasmalemmal caveolae in BMMCs. The involvement of caveolae in bacterial entry into BMMCs was indicated because caveolae-disrupting and -usurping agents specifically blocked E. coli entry, and markers of caveolae were actively recruited to sites of bacterial entry. The formation of bacteria-encapsulating caveolar chambers in BMMCs represents a distinct mechanism of microbial entry into phagocytes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shin, J S -- Gao, Z -- Abraham, S N -- AI 35678/AI/NIAID NIH HHS/ -- CA 14236/CA/NCI NIH HHS/ -- DK 50814/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 2000 Aug 4;289(5480):785-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Departments of Pathology and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10926542" target="_blank"〉PubMed〈/a〉
    Keywords: Adhesins, Bacterial/*metabolism ; *Adhesins, Escherichia coli ; Animals ; Antigens, CD/analysis/*metabolism ; Bacterial Adhesion ; Caveolin 1 ; *Caveolins ; Cell Fractionation ; Cell Membrane/chemistry/*metabolism/microbiology/ultrastructure ; Cholera Toxin/pharmacology ; Cyclodextrins/pharmacology ; *Endocytosis ; Escherichia coli/*metabolism/pathogenicity ; *Fimbriae Proteins ; Glycosylphosphatidylinositols/analysis ; Mast Cells/*metabolism/*microbiology/ultrastructure ; Membrane Proteins/analysis ; Mice ; Microscopy, Confocal ; Microscopy, Fluorescence ; Microscopy, Immunoelectron
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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