Publication Date:
2000-03-24
Description:
We constructed a bacterial artificial chromosome (BAC)-based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hoskins, R A -- Nelson, C R -- Berman, B P -- Laverty, T R -- George, R A -- Ciesiolka, L -- Naeemuddin, M -- Arenson, A D -- Durbin, J -- David, R G -- Tabor, P E -- Bailey, M R -- DeShazo, D R -- Catanese, J -- Mammoser, A -- Osoegawa, K -- de Jong, P J -- Celniker, S E -- Gibbs, R A -- Rubin, G M -- Scherer, S E -- HG00750/HG/NHGRI NIH HHS/ -- New York, N.Y. -- Science. 2000 Mar 24;287(5461):2271-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Berkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. hoskins@bdgp.lbl.gov〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10731150" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Centromere/genetics
;
Chromatin/genetics
;
Chromosomes, Bacterial/genetics
;
Cloning, Molecular
;
*Contig Mapping
;
DNA Fingerprinting
;
Drosophila melanogaster/*genetics
;
Euchromatin
;
Gene Library
;
Genes, Insect
;
Genetic Markers
;
Genetic Vectors
;
*Genome
;
In Situ Hybridization
;
Repetitive Sequences, Nucleic Acid
;
Restriction Mapping
;
Sequence Analysis, DNA
;
Sequence Tagged Sites
;
Telomere/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
